Rw5G027210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000497
Physical Location & Seq
Reverse (-)
39932819 .. 39933787
969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027210.1

Sequence Viewer

Length: 654 bp
ATGTTGAAGGTTGATTCATCTGCTGAAGGCTCACCTGTTGAAGAGAAACTAGTGGAGTCTGTTTTAGCAACATCGACCAAAGATGAGACTGGGGCGGCATGTTCATGTCAAAGGTTGCTAAATATGTGGAATGGCTTGAGGCCGTCATCTCAAAAAGTTTTGTCGCTAGTTGCTGAGTTGAAAACACTTCAGAAGAATAATGAAACACTTGAGAAGGATAAAGAACATCTAAGGATCAATCTGCTTTCTGCTGAAGAGGAGGTCAAATTGCTTTTCAAAGAAAACAAAGTGTTAGATGAAGCTAACAAAAGATTACTAAAGCAGTACCGCAAGGAAAGAAACAATTCTGGTTCTGATGGAAAGCATACTGATGTATCAACGAAGGTGAGAGAAAGGGAGAAGAATAGCTTTGATTGCATGTTTTACTATGCTCTTATACTCTCCTTGGCATTTCATGTCCATGAAATTGTAGTAGGATCTAATTTTATGCATTCTCCCCTTTTGCAGTCAAACAAGCGAAAATCTAGCTCTAAGGTGATAAGCAGCGGCCCGATTCAGGGGAAGATCGATTTCAGTGACCAAGAATCAGCAAGACAACCTGTCTCACCCTTGCGTTGTAACTCCCCTAACTCAAGACTGCATAAAAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

24.5

Weight (kDa)

9.2

Isoelectric Point (pI)

56.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14680 AT4G09060 AT4G09060
fragaria_vesca FvH4_5g36300 FvH4_5g36300
malus_domestica MD08G1189700.v1.1 MD08G1190000.v1.1 MD08G1190200.v1.1 MD15G1377500.v1.1
prunus_persica Prupe.1G524200_v2.0.a1
pyrus_communis pycom08g16320 pycom15g33820
rosa_chinensis RchiOBHm_Chr3g0481851 RchiOBHm_Chr3g0481861 RchiOBHm_Chr5g0040961 RchiOBHm_Chr5g0044491 RchiOBHm_Chr5g0044811 RchiOBHm_Chr5g0045611 RchiOBHm_Chr5g0045621 RchiOBHm_Chr5g0049341 RchiOBHm_Chr5g0049351 RchiOBHm_Chr7g0238301 RchiOBHm_Chr7g0238321
rosa_laevigata RLG00000000918 RLG00000000921 RLG00000034299 RLG00000034300
rosa_multiflora Rmu_sc0001047.1_g000051 Rmu_sc0001371.1_g000020 Rmu_sc0001847.1_g000002 Rmu_sc0002988.1_g000004 Rmu_sc0003789.1_g000001 Rmu_sc0004567.1_g000016 Rmu_sc0004800.1_g000003 Rmu_sc0005102.1_g000001 Rmu_sc0006651.1_g000010 Rmu_sc0006651.1_g000011 Rmu_ssc0000398.1_g000017
rosa_roxburghii Rroxscaffold_153G00436700 Rroxscaffold_153G00436710 Rroxscaffold_1G00034810 Rroxscaffold_1G00036220 Rroxscaffold_1G00036230 Rroxscaffold_1G00074790 Rroxscaffold_2G00104790 Rroxscaffold_2G00104800 Rroxscaffold_3G00223120 Rroxscaffold_5G00337240 Rroxscaffold_6G00399830 Rroxscaffold_6G00400170
rosa_rugosa Rorug02G0148000 Rorug04G0003000 Rorug05G0218300 Rorug05G0218400 Rorug05G0219900 Rorug07G0311100 Rorug07G0311900
rosa_samantha Rh2BG555200 Rh3DG293700 Rh4BG116500 Rh5BG279700 Rh5BG308000 Rh5BG308100 Rh5BG313100 Rh5BG313200 Rh5BG335300 Rh5BG335400 Rh5CG335000 Rh5CG335100 Rh5DG287700 Rh5DG318100 Rh5DG318200 Rh5DG323600 Rh5DG323700 Rh5DG347600 Rh5DG347700 Rh6DG317800 Rh7BG437500 Rh7CG484600 Rh7DG453000
rosa_wichuraiana Rw0G004340 Rw5G025810 Rw5G027210 Rw5G027900 Rw5G028300 Rw5G030640 Rw7G038670 Rw7G038780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 95, 328, 546
AclWI GGATC 2 cut(s) 242, 484
AcuI CTGAAG 3 cut(s) 45, 173, 273
AfaI GTAC 1 cut(s) 326
AfiI CCNNNNNNNGG 2 cut(s) 556, 557
AgsI TTSAA 4 cut(s) 7, 41, 181, 277
AhdI GACNNNNNGTC 1 cut(s) 599
AhlI ACTAGT 1 cut(s) 49
AluBI AGCT 3 cut(s) 302, 408, 528
AluI AGCT 3 cut(s) 302, 408, 528
Alw26I GTCTC 2 cut(s) 80, 607
AlwI GGATC 2 cut(s) 242, 484
AoxI GGCC 2 cut(s) 140, 547
ApeKI GCWGC 1 cut(s) 543
Asp700I GAANNNNTTC 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 548
AsuHPI GGTGA 4 cut(s) 24, 397, 547, 597
BaeI ACNNNNGTAYC 2 cut(s) 357, 390
BbvI GCAGC 1 cut(s) 555
BccI CCATC 1 cut(s) 350
BceAI ACGGC 1 cut(s) 127
BcoDI GTCTC 2 cut(s) 80, 607
BcuI ACTAGT 1 cut(s) 49
BfaI CTAG 3 cut(s) 50, 167, 525
BisI GCNGC 3 cut(s) 96, 544, 547
BlsI GCNGC 3 cut(s) 97, 545, 548
BmeRI GACNNNNNGTC 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 548
BmrI ACTGGG 1 cut(s) 99
BmuI ACTGGG 1 cut(s) 99
BpuEI CTTGAG 3 cut(s) 157, 230, 616
Bsa29I ATCGAT 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 444
Bsc4I CCNNNNNNNGG 2 cut(s) 556, 557
Bse1I ACTGG 1 cut(s) 94
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 1 cut(s) 444
BseLI CCNNNNNNNGG 2 cut(s) 556, 557
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 1 cut(s) 555
BshFI GGCC 2 cut(s) 142, 549
BshVI ATCGAT 1 cut(s) 567
BslI CCNNNNNNNGG 2 cut(s) 556, 557
BsmAI GTCTC 2 cut(s) 80, 607
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 2 cut(s) 142, 549
Bsp143I GATC 3 cut(s) 234, 476, 564
BspACI CCGC 3 cut(s) 95, 328, 546
BspANI GGCC 2 cut(s) 142, 549
BspCNI CTCAG 1 cut(s) 166
BspDI ATCGAT 1 cut(s) 567
BspPI GGATC 2 cut(s) 242, 484
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 3 cut(s) 234, 476, 564
BssT1I CCWWGG 1 cut(s) 444
Bst6I CTCTTC 2 cut(s) 36, 249
BstDEI CTNAG 3 cut(s) 174, 230, 531
BstKTI GATC 3 cut(s) 237, 479, 567
BstMAI GTCTC 2 cut(s) 80, 607
BstMBI GATC 3 cut(s) 234, 476, 564
BstMWI GCNNNNNNNGC 2 cut(s) 414, 646
BstNSI RCATGY 2 cut(s) 102, 421
BstV1I GCAGC 1 cut(s) 555
BstX2I RGATCY 1 cut(s) 476
BstYI RGATCY 1 cut(s) 476
Bsu15I ATCGAT 1 cut(s) 567
BsuRI GGCC 2 cut(s) 142, 549
BsuTUI ATCGAT 1 cut(s) 567
BtsIMutI CAGTG 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 548
ClaI ATCGAT 1 cut(s) 567
Csp6I GTAC 1 cut(s) 325
CviAII CATG 5 cut(s) 99, 105, 418, 455, 461
CviJI RGCY 7 cut(s) 30, 135, 142, 302, 408, 528, 549
CviKI_1 RGCY 7 cut(s) 30, 135, 142, 302, 408, 528, 549
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 3 cut(s) 174, 230, 531
DpnI GATC 3 cut(s) 236, 478, 566
DpnII GATC 3 cut(s) 234, 476, 564
DriI GACNNNNNGTC 1 cut(s) 599
Eam1104I CTCTTC 2 cut(s) 36, 249
Eam1105I GACNNNNNGTC 1 cut(s) 599
EarI CTCTTC 2 cut(s) 36, 249
Eco130I CCWWGG 1 cut(s) 444
Eco57I CTGAAG 3 cut(s) 45, 173, 273
EcoT14I CCWWGG 1 cut(s) 444
EcoT22I ATGCAT 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 5 cut(s) 102, 108, 421, 458, 464
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FatI CATG 5 cut(s) 98, 104, 417, 454, 460
Fnu4HI GCNGC 3 cut(s) 96, 544, 547
Fsp4HI GCNGC 3 cut(s) 96, 544, 547
FspBI CTAG 3 cut(s) 50, 167, 525
GluI GCNGC 3 cut(s) 96, 544, 547
HaeIII GGCC 2 cut(s) 142, 549
Hin1II CATG 5 cut(s) 102, 108, 421, 458, 464
HinfI GANTC 4 cut(s) 14, 56, 553, 584
HphI GGTGA 4 cut(s) 24, 397, 547, 597
Hpy188I TCNGA 2 cut(s) 192, 355
Hpy188III TCNNGA 1 cut(s) 633
HpyAV CCTTC 3 cut(s) 20, 208, 376
HpyCH4V TGCA 4 cut(s) 417, 490, 505, 640
HpyF10VI GCNNNNNNNGC 2 cut(s) 414, 646
HpyF3I CTNAG 3 cut(s) 174, 230, 531
Hsp92II CATG 5 cut(s) 102, 108, 421, 458, 464
Kzo9I GATC 3 cut(s) 234, 476, 564
LpnPI CCDG 5 cut(s) 48, 75, 333, 542, 612
Lsp1109I GCAGC 1 cut(s) 555
MaeI CTAG 3 cut(s) 50, 167, 525
MaeIII GTNAC 2 cut(s) 575, 617
MalI GATC 3 cut(s) 236, 478, 566
MboI GATC 3 cut(s) 234, 476, 564
MboII GAAGA 5 cut(s) 53, 205, 266, 412, 574
MflI RGATCY 1 cut(s) 476
MluCI AATT 4 cut(s) 266, 343, 465, 481
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 3 cut(s) 132, 250, 253
Mph1103I ATGCAT 1 cut(s) 492
MroXI GAANNNNTTC 1 cut(s) 343
MslI CAYNNNNRTG 3 cut(s) 103, 369, 459
MspA1I CMGCKG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 490
MwoI GCNNNNNNNGC 2 cut(s) 414, 646
NdeII GATC 3 cut(s) 234, 476, 564
NlaIII CATG 5 cut(s) 102, 108, 421, 458, 464
NmuCI GTSAC 1 cut(s) 575
NsiI ATGCAT 1 cut(s) 492
NspI RCATGY 2 cut(s) 102, 421
PctI GAATGC 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 343
PfeI GAWTC 3 cut(s) 14, 553, 584
PkrI GCNGC 3 cut(s) 97, 545, 548
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
PspPI GGNCC 1 cut(s) 548
PsuI RGATCY 1 cut(s) 476
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 3 cut(s) 103, 369, 459
SatI GCNGC 3 cut(s) 96, 544, 547
Sau3AI GATC 3 cut(s) 234, 476, 564
Sau96I GGNCC 1 cut(s) 548
SchI GAGTC 1 cut(s) 65
SmiMI CAYNNNNRTG 3 cut(s) 103, 369, 459
SmlI CTYRAG 3 cut(s) 136, 209, 631
SmoI CTYRAG 3 cut(s) 136, 209, 631
SpeI ACTAGT 1 cut(s) 49
Sse9I AATT 4 cut(s) 266, 343, 465, 481
SsiI CCGC 3 cut(s) 95, 328, 546
SspMI CTAG 3 cut(s) 50, 167, 525
StyI CCWWGG 1 cut(s) 444
TaqI TCGA 2 cut(s) 74, 567
TasI AATT 4 cut(s) 266, 343, 465, 481
TauI GCSGC 2 cut(s) 98, 549
TfiI GAWTC 3 cut(s) 14, 553, 584
TscAI CASTG 1 cut(s) 580
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 1 cut(s) 543
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 6 cut(s) 6, 93, 216, 312, 443, 477
TspRI CASTG 1 cut(s) 580
XceI RCATGY 2 cut(s) 102, 421
XmnI GAANNNNTTC 1 cut(s) 343
XspI CTAG 3 cut(s) 50, 167, 525
Zsp2I ATGCAT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.