Rmu_sc0008380.1_g000005

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008380.1
OG0000589
Physical Location & Seq
Forward (+)
29077 .. 31853
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008380.1_g000005.1.cds

Sequence Viewer

Length: 753 bp
atgcctccattatcattgagccatacttcaggggtgaattccaaagctttctttggtgggacgacgacgactagtgtattacgcaaacgcaggcctggctgcgcattgttttctttgaagcctcaaacaatggtggggttaactctggcaaatctaaaactcagacgttacagaaacaatgaacgaatctttgtgaatcctgatgaatgcagtgatactaagactaatcacagtcatgccagggtagtgcagtgggggggcttgaagggcgccttctctaggcatcttcctgcaatccaaagcgaggcagaagcttgtacagaaggcttgcttgtggctattcaccagagttggagacatgttgaggttgaaagtgattgtgctcagttgcttattgcgctgaatcttcaaggggaggataactctgaggaaaattgtagaattgcaatcgaggcacgagaaatttactatgacaaatgcaccaaacgttcttgggaaattgtgaacacaggaaagccttcaagacatccagttctatttgcagcatctgtgggaagctatggagcctatttggctgatggttccgagtatagtggcaactgtggcgatgcagtgactctagaaacattgaaagatttccacagggaaggggttcagattctagccaactctgttgctcatctaattgcatttgagacgacactgaataagttagaggcaaaggcaatatgctttattcgccttaatgtataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

250

Amino Acids

27.61

Weight (kDa)

8.11

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000589)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22690 AT3G22690 AT3G22690
fragaria_vesca FvH4_4g12100 FvH4_4g12100 FvH4_4g12100
malus_domestica MD07G1198500.v1.1 MD12G1181700.v1.1
prunus_persica Prupe.1G018800_v2.0.a1
pyrus_communis pycom07g16490
rosa_chinensis RchiOBHm_Chr1g0328851 RchiOBHm_Chr1g0329061 RchiOBHm_Chr1g0329101 RchiOBHm_Chr1g0329141 RchiOBHm_Chr2g0124781 RchiOBHm_Chr3g0482411 RchiOBHm_Chr3g0482461 RchiOBHm_Chr4g0386511 RchiOBHm_Chr4g0386531 RchiOBHm_Chr4g0386651 RchiOBHm_Chr4g0386671 RchiOBHm_Chr4g0386751 RchiOBHm_Chr4g0386781 RchiOBHm_Chr4g0386861 RchiOBHm_Chr4g0386891 RchiOBHm_Chr4g0386911 RchiOBHm_Chr4g0386991 RchiOBHm_Chr4g0387041 RchiOBHm_Chr4g0387171 RchiOBHm_Chr4g0387241 RchiOBHm_Chr4g0387301 RchiOBHm_Chr4g0387341 RchiOBHm_Chr4g0387441 RchiOBHm_Chr4g0388061 RchiOBHm_Chr4g0389241 RchiOBHm_Chr4g0389361
rosa_laevigata RLG00000010137 RLG00000010138 RLG00000010145 RLG00000010146 RLG00000018820 RLG00000023352 RLG00000029915
rosa_multiflora Rmu_co8009444.1_g000001 Rmu_co8455349.1_g000001 Rmu_sc0001375.1_g000001 Rmu_sc0001942.1_g000049 Rmu_sc0001942.1_g000077 Rmu_sc0002939.1_g000001 Rmu_sc0003413.1_g000068 Rmu_sc0003413.1_g000075 Rmu_sc0006571.1_g000008 Rmu_sc0006571.1_g000013 Rmu_sc0006571.1_g000016 Rmu_sc0006648.1_g000004 Rmu_sc0008380.1_g000005 Rmu_sc0009161.1_g000006 Rmu_sc0009161.1_g000007 Rmu_sc0010272.1_g000019 Rmu_sc0011657.1_g000016 Rmu_sc0028698.1_g000001
rosa_roxburghii Rroxscaffold_2G00119680 Rroxscaffold_2G00119690 Rroxscaffold_5G00334120
rosa_rugosa Rorug02G0252500 Rorug02G0252500 Rorug03G0196200 Rorug03G0307500 Rorug03G0307800 Rorug03G0308200 Rorug03G0308400 Rorug03G0365200.1 Rorug07G0142900
rosa_samantha Rh2AG312000 Rh2BG320300 Rh2CG300300 Rh4DG011500 Rh4DG020300
rosa_wichuraiana Rw2G025200 Rw3G022270 Rw4G001010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 103
AccB1I GGYRCC 1 cut(s) 269
AclI AACGTT 1 cut(s) 487
AcsI RAATTY 2 cut(s) 37, 462
AcuI CTGAAG 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 2 cut(s) 279, 304
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 6 cut(s) 118, 265, 371, 410, 522, 631
AhlI ACTAGT 1 cut(s) 71
AjnI CCWGG 2 cut(s) 94, 239
AluBI AGCT 3 cut(s) 47, 314, 558
AluI AGCT 3 cut(s) 47, 314, 558
Alw21I GWGCWC 1 cut(s) 385
Alw26I GTCTC 2 cut(s) 349, 689
AlwNI CAGNNNCTG 1 cut(s) 548
AoxI GGCC 1 cut(s) 92
ApeKI GCWGC 2 cut(s) 99, 542
ApoI RAATTY 2 cut(s) 37, 462
Asp700I GAANNNNTTC 3 cut(s) 517, 635, 651
AspLEI GCGC 3 cut(s) 104, 272, 400
AsuHPI GGTGA 2 cut(s) 46, 335
BanI GGYRCC 1 cut(s) 269
BauI CACGAG 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 385
BbvI GCAGC 2 cut(s) 86, 554
BccI CCATC 1 cut(s) 572
BciT130I CCWGG 2 cut(s) 96, 241
BcoDI GTCTC 2 cut(s) 349, 689
BcuI ACTAGT 1 cut(s) 71
BfaI CTAG 4 cut(s) 72, 279, 620, 662
BfoI RGCGCY 1 cut(s) 273
BglI GCCNNNNNGGC 1 cut(s) 572
BisI GCNGC 2 cut(s) 100, 543
BlsI GCNGC 2 cut(s) 101, 544
Bme1390I CCNGG 2 cut(s) 96, 241
BmiI GGNNCC 3 cut(s) 271, 565, 583
BmrFI CCNGG 2 cut(s) 96, 241
BmsI GCATC 3 cut(s) 292, 554, 598
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 304
Bse1I ACTGG 1 cut(s) 530
BseBI CCWGG 2 cut(s) 96, 241
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 2 cut(s) 279, 304
BseMII CTCAG 3 cut(s) 175, 398, 417
BseNI ACTGG 1 cut(s) 530
BseXI GCAGC 2 cut(s) 86, 554
BsgI GTGCAG 1 cut(s) 269
BshFI GGCC 1 cut(s) 94
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 385
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 279, 304
BsmAI GTCTC 2 cut(s) 349, 689
BsmBI CGTCTC 1 cut(s) 689
BsmFI GGGAC 1 cut(s) 73
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 385
Bsp1407I TGTACA 1 cut(s) 317
BspANI GGCC 1 cut(s) 94
BspCNI CTCAG 3 cut(s) 174, 397, 418
BspLI GGNNCC 3 cut(s) 271, 565, 583
BspT107I GGYRCC 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 317
BsrI ACTGG 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 240
BssNI GRCGYC 1 cut(s) 270
BssSI CACGAG 1 cut(s) 456
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 96, 241
Bst4CI ACNGT 2 cut(s) 233, 602
BstACI GRCGYC 1 cut(s) 270
BstAUI TGTACA 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 92, 329
BstDEI CTNAG 4 cut(s) 161, 219, 384, 426
BstENI CCTNNNNNAGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 526
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 3 cut(s) 104, 272, 400
BstMAI GTCTC 2 cut(s) 349, 689
BstMWI GCNNNNNNNGC 7 cut(s) 96, 267, 397, 452, 572, 603, 738
BstNI CCWGG 2 cut(s) 96, 241
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 2 cut(s) 94, 239
BstV1I GCAGC 2 cut(s) 86, 554
BsuRI GGCC 1 cut(s) 94
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 1 cut(s) 526
BtsI GCAGTG 3 cut(s) 217, 257, 618
BtsIMutI CAGTG 4 cut(s) 217, 257, 618, 701
Cac8I GCNNGC 2 cut(s) 92, 329
CaiI CAGNNNCTG 1 cut(s) 548
CfoI GCGC 3 cut(s) 104, 272, 400
Csp6I GTAC 1 cut(s) 318
CviAII CATG 2 cut(s) 236, 359
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 4 cut(s) 161, 219, 384, 426
DinI GGCGCC 1 cut(s) 271
Eco147I AGGCCT 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 12
EcoNI CCTNNNNNAGG 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 37
EcoRII CCWGG 2 cut(s) 94, 239
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 689
FaeI CATG 2 cut(s) 239, 362
FaiI YATR 8 cut(s) 24, 237, 360, 471, 561, 591, 730, 751
FalI AAGNNNNNCTT 4 cut(s) 257, 289, 315, 347
FaqI GGGAC 1 cut(s) 73
FatI CATG 2 cut(s) 235, 358
Fnu4HI GCNGC 2 cut(s) 100, 543
FokI GGATG 1 cut(s) 513
Fsp4HI GCNGC 2 cut(s) 100, 543
FspBI CTAG 4 cut(s) 72, 279, 620, 662
FspI TGCGCA 1 cut(s) 103
GlaI GCGC 3 cut(s) 103, 271, 399
GluI GCNGC 2 cut(s) 100, 543
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 1 cut(s) 94
HhaI GCGC 3 cut(s) 104, 272, 400
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 2 cut(s) 239, 362
Hin6I GCGC 3 cut(s) 102, 270, 398
HinP1I GCGC 3 cut(s) 102, 270, 398
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HindIII AAGCTT 2 cut(s) 45, 312
HinfI GANTC 5 cut(s) 186, 196, 403, 616, 658
HpaI GTTAAC 1 cut(s) 141
HphI GGTGA 2 cut(s) 46, 335
Hpy166II GTNNAC 2 cut(s) 141, 505
Hpy188I TCNGA 4 cut(s) 164, 427, 586, 657
Hpy188III TCNNGA 3 cut(s) 200, 522, 620
Hpy8I GTNNAC 2 cut(s) 141, 505
Hpy99I CGWCG 2 cut(s) 67, 70
HpyAV CCTTC 5 cut(s) 259, 283, 317, 528, 641
HpyCH4III ACNGT 2 cut(s) 233, 602
HpyCH4IV ACGT 2 cut(s) 166, 487
HpyCH4V TGCA 8 cut(s) 210, 250, 293, 446, 480, 542, 611, 689
HpyF10VI GCNNNNNNNGC 7 cut(s) 96, 267, 397, 452, 572, 603, 738
HpyF3I CTNAG 4 cut(s) 161, 219, 384, 426
HpySE526I ACGT 2 cut(s) 166, 487
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 2 cut(s) 239, 362
HspAI GCGC 3 cut(s) 102, 270, 398
KasI GGCGCC 1 cut(s) 269
KspAI GTTAAC 1 cut(s) 141
LmnI GCTCC 1 cut(s) 563
Lsp1109I GCAGC 2 cut(s) 86, 554
LweI GCATC 3 cut(s) 292, 554, 598
MaeI CTAG 4 cut(s) 72, 279, 620, 662
MaeII ACGT 2 cut(s) 166, 487
MaeIII GTNAC 2 cut(s) 167, 613
MboII GAAGA 2 cut(s) 278, 398
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 6 cut(s) 37, 433, 441, 462, 498, 684
Mly113I GGCGCC 1 cut(s) 270
MlyI GAGTC 1 cut(s) 610
MmeI TCCRAC 1 cut(s) 332
MnlI CCTC 8 cut(s) 15, 132, 298, 358, 409, 421, 445, 709
MroXI GAANNNNTTC 3 cut(s) 517, 635, 651
MseI TTAA 2 cut(s) 140, 744
MslI CAYNNNNRTG 1 cut(s) 234
MspR9I CCNGG 2 cut(s) 96, 241
Mva1269I GAATGC 1 cut(s) 212
MvaI CCWGG 2 cut(s) 96, 241
MwoI GCNNNNNNNGC 7 cut(s) 96, 267, 397, 452, 572, 603, 738
NarI GGCGCC 1 cut(s) 270
NlaIII CATG 2 cut(s) 239, 362
NlaIV GGNNCC 3 cut(s) 271, 565, 583
NmuCI GTSAC 1 cut(s) 613
NsbI TGCGCA 1 cut(s) 103
NspI RCATGY 1 cut(s) 362
PceI AGGCCT 1 cut(s) 94
PciI ACATGT 1 cut(s) 358
PctI GAATGC 1 cut(s) 212
PdmI GAANNNNTTC 3 cut(s) 517, 635, 651
PfeI GAWTC 4 cut(s) 186, 196, 403, 658
PkrI GCNGC 2 cut(s) 101, 544
PleI GAGTC 1 cut(s) 610
PluTI GGCGCC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 610
PscI ACATGT 1 cut(s) 358
Psp1406I AACGTT 1 cut(s) 487
Psp6I CCWGG 2 cut(s) 94, 239
PspGI CCWGG 2 cut(s) 94, 239
PspN4I GGNNCC 3 cut(s) 271, 565, 583
PstNI CAGNNNCTG 1 cut(s) 548
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 2 cut(s) 140, 744
SatI GCNGC 2 cut(s) 100, 543
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 2 cut(s) 96, 241
SduI GDGCHC 1 cut(s) 385
SetI ASST 6 cut(s) 49, 169, 316, 369, 490, 560
SfaNI GCATC 3 cut(s) 292, 554, 598
SfoI GGCGCC 1 cut(s) 271
SmiMI CAYNNNNRTG 1 cut(s) 234
SpeI ACTAGT 1 cut(s) 71
Sse9I AATT 6 cut(s) 37, 433, 441, 462, 498, 684
SseBI AGGCCT 1 cut(s) 94
SspDI GGCGCC 1 cut(s) 269
SspMI CTAG 4 cut(s) 72, 279, 620, 662
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 2 cut(s) 94, 239
TaaI ACNGT 2 cut(s) 233, 602
TaiI ACGT 2 cut(s) 169, 490
TaqI TCGA 1 cut(s) 450
TasI AATT 6 cut(s) 37, 433, 441, 462, 498, 684
TatI WGTACW 1 cut(s) 317
TfiI GAWTC 4 cut(s) 186, 196, 403, 658
Tru1I TTAA 2 cut(s) 140, 744
Tru9I TTAA 2 cut(s) 140, 744
TscAI CASTG 4 cut(s) 217, 257, 618, 708
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 2 cut(s) 99, 542
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 2 cut(s) 195, 219
TspRI CASTG 4 cut(s) 217, 257, 618, 708
XagI CCTNNNNNAGG 1 cut(s) 277
XapI RAATTY 2 cut(s) 37, 462
XbaI TCTAGA 1 cut(s) 619
XceI RCATGY 1 cut(s) 362
XmnI GAANNNNTTC 3 cut(s) 517, 635, 651
XspI CTAG 4 cut(s) 72, 279, 620, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.