Rmu_sc0009161.1_g000007

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009161.1
OG0000589
Physical Location & Seq
Reverse (-)
55747 .. 58221
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009161.1_g000007.1.cds

Sequence Viewer

Length: 657 bp
atgcttgagagaaacgctgtgtcgtggactagtttcatttgtggttatggttggaggaacatgccaaaggaggctgctttctttgtttttcgagatggtgtggggactatagattttccattcagaaaggttacacaatctggccaagttggctacatgctgattgctgatctaactgaatttgagacgaccgccaatgagctagaagcaaagcttaaactcagatgttccagaaacaatgaacgaatctttgtgaatcctaatgaatgcagtgatactaagattaatcacagtcatgccagggtagtgcagtggggaggcttgaagggcgccttctctaggcatcttcctgcaatccaaagcgaggcagaaaattgtagagcaggcttgcttgtggctattcaccagagttggagacatgttgaggtagaaagtgattgtgctcagttgcttgctgtgctgaatcttcaaggggaggataactctgaggaaaattgtagaattgcaatcgaggcatcagaaatttactatgacaaatgcaccaaaggttcttgggaaattgtggacacacgaaagccttcaagacatccagttctagttgcggcatctgtgggaagctgtggagcctatttgactgatggttctgagtataggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

218

Amino Acids

24.47

Weight (kDa)

5.9

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000589)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22690 AT3G22690 AT3G22690
fragaria_vesca FvH4_4g12100 FvH4_4g12100 FvH4_4g12100
malus_domestica MD07G1198500.v1.1 MD12G1181700.v1.1
prunus_persica Prupe.1G018800_v2.0.a1
pyrus_communis pycom07g16490
rosa_chinensis RchiOBHm_Chr1g0328851 RchiOBHm_Chr1g0329061 RchiOBHm_Chr1g0329101 RchiOBHm_Chr1g0329141 RchiOBHm_Chr2g0124781 RchiOBHm_Chr3g0482411 RchiOBHm_Chr3g0482461 RchiOBHm_Chr4g0386511 RchiOBHm_Chr4g0386531 RchiOBHm_Chr4g0386651 RchiOBHm_Chr4g0386671 RchiOBHm_Chr4g0386751 RchiOBHm_Chr4g0386781 RchiOBHm_Chr4g0386861 RchiOBHm_Chr4g0386891 RchiOBHm_Chr4g0386911 RchiOBHm_Chr4g0386991 RchiOBHm_Chr4g0387041 RchiOBHm_Chr4g0387171 RchiOBHm_Chr4g0387241 RchiOBHm_Chr4g0387301 RchiOBHm_Chr4g0387341 RchiOBHm_Chr4g0387441 RchiOBHm_Chr4g0388061 RchiOBHm_Chr4g0389241 RchiOBHm_Chr4g0389361
rosa_laevigata RLG00000010137 RLG00000010138 RLG00000010145 RLG00000010146 RLG00000018820 RLG00000023352 RLG00000029915
rosa_multiflora Rmu_co8009444.1_g000001 Rmu_co8455349.1_g000001 Rmu_sc0001375.1_g000001 Rmu_sc0001942.1_g000049 Rmu_sc0001942.1_g000077 Rmu_sc0002939.1_g000001 Rmu_sc0003413.1_g000068 Rmu_sc0003413.1_g000075 Rmu_sc0006571.1_g000008 Rmu_sc0006571.1_g000013 Rmu_sc0006571.1_g000016 Rmu_sc0006648.1_g000004 Rmu_sc0008380.1_g000005 Rmu_sc0009161.1_g000006 Rmu_sc0009161.1_g000007 Rmu_sc0010272.1_g000019 Rmu_sc0011657.1_g000016 Rmu_sc0028698.1_g000001
rosa_roxburghii Rroxscaffold_2G00119680 Rroxscaffold_2G00119690 Rroxscaffold_5G00334120
rosa_rugosa Rorug02G0252500 Rorug02G0252500 Rorug03G0196200 Rorug03G0307500 Rorug03G0307800 Rorug03G0308200 Rorug03G0308400 Rorug03G0365200.1 Rorug07G0142900
rosa_samantha Rh2AG312000 Rh2BG320300 Rh2CG300300 Rh4DG011500 Rh4DG020300
rosa_wichuraiana Rw2G025200 Rw3G022270 Rw4G001010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 329
AciI CCGC 2 cut(s) 192, 602
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 2 cut(s) 179, 522
AcyI GRCGYC 1 cut(s) 330
AfiI CCNNNNNNNGG 2 cut(s) 339, 364
AflIII ACRYGT 1 cut(s) 418
AgsI TTSAA 3 cut(s) 325, 470, 582
AhlI ACTAGT 1 cut(s) 29
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 3 cut(s) 202, 214, 618
AluI AGCT 3 cut(s) 202, 214, 618
Alw21I GWGCWC 1 cut(s) 445
Alw26I GTCTC 2 cut(s) 179, 409
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 2 cut(s) 179, 522
AseI ATTAAT 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 577
AspLEI GCGC 1 cut(s) 332
AsuHPI GGTGA 1 cut(s) 395
BalI TGGCCA 1 cut(s) 144
BanI GGYRCC 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 445
BbvI GCAGC 1 cut(s) 61
BccI CCATC 2 cut(s) 89, 632
BciT130I CCWGG 1 cut(s) 301
BcoDI GTCTC 2 cut(s) 179, 409
BcuI ACTAGT 1 cut(s) 29
BfaI CTAG 4 cut(s) 30, 203, 339, 596
BfmI CTRYAG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 333
BglI GCCNNNNNGGC 1 cut(s) 150
BisI GCNGC 2 cut(s) 75, 603
BlsI GCNGC 2 cut(s) 76, 604
Bme1390I CCNGG 1 cut(s) 301
BmiI GGNNCC 2 cut(s) 331, 625
BmrFI CCNGG 1 cut(s) 301
BmsI GCATC 3 cut(s) 352, 524, 614
BplI GAGNNNNNCTC 2 cut(s) 467, 499
BpuEI CTTGAG 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 300
Bsc4I CCNNNNNNNGG 2 cut(s) 339, 364
Bse1I ACTGG 1 cut(s) 590
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 2 cut(s) 339, 364
BseMII CTCAG 4 cut(s) 235, 458, 477, 636
BseNI ACTGG 1 cut(s) 590
BseXI GCAGC 1 cut(s) 61
BsgI GTGCAG 1 cut(s) 329
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 445
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 2 cut(s) 339, 364
BsmAI GTCTC 2 cut(s) 179, 409
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 118
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 445
Bsp143I GATC 1 cut(s) 169
BspACI CCGC 2 cut(s) 192, 602
BspANI GGCC 1 cut(s) 144
BspCNI CTCAG 4 cut(s) 234, 457, 478, 637
BspLI GGNNCC 2 cut(s) 331, 625
BspT107I GGYRCC 1 cut(s) 329
BsrI ACTGG 1 cut(s) 590
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 1 cut(s) 169
BssNI GRCGYC 1 cut(s) 330
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 330
BstC8I GCNNGC 3 cut(s) 385, 389, 453
BstDEI CTNAG 5 cut(s) 221, 279, 444, 486, 645
BstENI CCTNNNNNAGG 1 cut(s) 337
BstF5I GGATG 1 cut(s) 586
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 1 cut(s) 172
BstMAI GTCTC 2 cut(s) 179, 409
BstMBI GATC 1 cut(s) 169
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 4 cut(s) 150, 327, 457, 512
BstNI CCWGG 1 cut(s) 301
BstNSI RCATGY 3 cut(s) 64, 160, 422
BstSCI CCNGG 1 cut(s) 299
BstSFI CTRYAG 1 cut(s) 108
BstV1I GCAGC 1 cut(s) 61
BsuRI GGCC 1 cut(s) 144
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 2 cut(s) 277, 317
BtsIMutI CAGTG 2 cut(s) 277, 317
Cac8I GCNNGC 3 cut(s) 385, 389, 453
CfoI GCGC 1 cut(s) 332
CviAII CATG 4 cut(s) 61, 157, 296, 419
DdeI CTNAG 5 cut(s) 221, 279, 444, 486, 645
DinI GGCGCC 1 cut(s) 331
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 142
EcoNI CCTNNNNNAGG 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 299
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 4 cut(s) 64, 160, 299, 422
FaiI YATR 8 cut(s) 48, 62, 110, 158, 297, 420, 531, 651
FalI AAGNNNNNCTT 4 cut(s) 198, 230, 317, 349
FaqI GGGAC 1 cut(s) 118
FatI CATG 4 cut(s) 60, 156, 295, 418
Fnu4HI GCNGC 2 cut(s) 75, 603
FokI GGATG 1 cut(s) 573
Fsp4HI GCNGC 2 cut(s) 75, 603
FspBI CTAG 4 cut(s) 30, 203, 339, 596
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 75, 603
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 1 cut(s) 144
HhaI GCGC 1 cut(s) 332
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 4 cut(s) 64, 160, 299, 422
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 3 cut(s) 246, 256, 463
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 2 cut(s) 27, 565
Hpy188I TCNGA 5 cut(s) 125, 224, 487, 520, 646
Hpy188III TCNNGA 3 cut(s) 92, 231, 582
Hpy8I GTNNAC 2 cut(s) 27, 565
HpyAV CCTTC 3 cut(s) 319, 343, 588
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4V TGCA 5 cut(s) 270, 310, 353, 506, 540
HpyF10VI GCNNNNNNNGC 4 cut(s) 150, 327, 457, 512
HpyF3I CTNAG 5 cut(s) 221, 279, 444, 486, 645
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 4 cut(s) 64, 160, 299, 422
HspAI GCGC 1 cut(s) 330
KasI GGCGCC 1 cut(s) 329
Kzo9I GATC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 623
LpnPI CCDG 8 cut(s) 126, 244, 286, 313, 363, 369, 419, 603
Lsp1109I GCAGC 1 cut(s) 61
LweI GCATC 3 cut(s) 352, 524, 614
MaeI CTAG 4 cut(s) 30, 203, 339, 596
MaeIII GTNAC 1 cut(s) 130
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 2 cut(s) 338, 458
MhlI GDGCHC 1 cut(s) 445
MlsI TGGCCA 1 cut(s) 144
MluCI AATT 6 cut(s) 179, 373, 493, 501, 522, 558
MluNI TGGCCA 1 cut(s) 144
Mly113I GGCGCC 1 cut(s) 330
MmeI TCCRAC 2 cut(s) 32, 392
MnlI CCTC 8 cut(s) 48, 64, 311, 358, 418, 469, 481, 505
Mox20I TGGCCA 1 cut(s) 144
MroXI GAANNNNTTC 1 cut(s) 577
MscI TGGCCA 1 cut(s) 144
MseI TTAA 2 cut(s) 216, 285
MslI CAYNNNNRTG 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 144
MspR9I CCNGG 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 1 cut(s) 301
MwoI GCNNNNNNNGC 4 cut(s) 150, 327, 457, 512
NarI GGCGCC 1 cut(s) 330
NdeII GATC 1 cut(s) 169
NlaIII CATG 4 cut(s) 64, 160, 299, 422
NlaIV GGNNCC 2 cut(s) 331, 625
NspI RCATGY 3 cut(s) 64, 160, 422
PciI ACATGT 1 cut(s) 418
PctI GAATGC 1 cut(s) 272
PdmI GAANNNNTTC 1 cut(s) 577
PfeI GAWTC 3 cut(s) 246, 256, 463
PkrI GCNGC 2 cut(s) 76, 604
PluTI GGCGCC 1 cut(s) 333
PscI ACATGT 1 cut(s) 418
PshBI ATTAAT 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 331, 625
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 2 cut(s) 216, 285
SatI GCNGC 2 cut(s) 75, 603
Sau3AI GATC 1 cut(s) 169
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 1 cut(s) 445
SetI ASST 7 cut(s) 132, 204, 216, 429, 550, 620, 656
SfaNI GCATC 3 cut(s) 352, 524, 614
SfcI CTRYAG 1 cut(s) 108
SfoI GGCGCC 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
SpeI ACTAGT 1 cut(s) 29
Sse9I AATT 6 cut(s) 179, 373, 493, 501, 522, 558
SsiI CCGC 2 cut(s) 192, 602
SspDI GGCGCC 1 cut(s) 329
SspMI CTAG 4 cut(s) 30, 203, 339, 596
StyD4I CCNGG 1 cut(s) 299
TaaI ACNGT 1 cut(s) 293
TaqI TCGA 2 cut(s) 91, 510
TasI AATT 6 cut(s) 179, 373, 493, 501, 522, 558
TauI GCSGC 1 cut(s) 605
TfiI GAWTC 3 cut(s) 246, 256, 463
Tru1I TTAA 2 cut(s) 216, 285
Tru9I TTAA 2 cut(s) 216, 285
TscAI CASTG 2 cut(s) 277, 317
TseI GCWGC 1 cut(s) 74
TspDTI ATGAA 3 cut(s) 25, 255, 279
TspRI CASTG 2 cut(s) 277, 317
VspI ATTAAT 1 cut(s) 285
XagI CCTNNNNNAGG 1 cut(s) 337
XapI RAATTY 2 cut(s) 179, 522
XceI RCATGY 3 cut(s) 64, 160, 422
XmnI GAANNNNTTC 1 cut(s) 577
XspI CTAG 4 cut(s) 30, 203, 339, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.