Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0001045

Physical Location & Seq

Genomic Coordinates Reverse (-)

7875695 .. 7888410

Gene Structure 12716 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG053500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1242 bp

ATGCAGAGCATTGAGGCAATTGCAACTGATTTGGCATACTTGTATGGCCACTACCCCAAGCTCAGTGATACCAATGTCTTTCGTGTTCTAGCTACTGCTTCTTTTCTTGATCTTCAGGTTTGCAAATCCATGAAATTTCTAGTTGGTGGTGCTTCCTTGCAGTTTGAGCAAAAGGCAAATGAGGTGGTGATCGAGCTTCCTTGGGTCAAGAATGTCATTGTGACTATGTCAGCACAACCTGCACAACCCCTTTATGCCGGGCAACTTCCACAAGAGTTACAGACAATTTCAAATATTGTGGCAGTTTCTAGTTGCAAGAGACCAGCTTCTAACTACAACAGAGTGGATTATGTGCCTACAGTGTTTGACAACATCAGTGCTAATTTGGTGGCGGATGCACAGTTAACCTTGGATTGTGGGACACTGCAGAACAATGAGAAACTGACTTGCTCGAGCTGCAGGGAGCTTGCACTTCAGATTGATCAGCAGTTCCAAGCTCTTGGGCCTGGCATTGGCGCCAAGTCTATAGCGCTTGTTGGAGGAGAGTGTGAGGTGCAACAATCTCCTGTTCTTGCAAAGCGGCCGCATATTATTGTTGCAATACCTGGACGCTTCCTTCATCATCTAACCAAGACAAGAGGATTCTCTCTAGGCACAATTAAATATTTGGTTTTAGATGAGGCAGACAGGTTGCTTGGTGAGGAGTTTCAGAAAACAACAAATGAAATTCTGAAGGTCATTCCTCATGGTGGTGAACGGAGAACATATTTATTTTCCACTACTATGACCAACAAGGTCAAGAAGGTGGCAAGGGCTGGTCTAAGGAACCCTGTGAAGATTGAAGTAGCATCGAAGTATTCTACAGTTGAAACCCTACAGCAGCAAATGATGAGTACACCAGTGCAGTACAAGGTGATGATTTATAGGCTAATAACACGAGGAACAATTGAAGAAAGAATGATGCAACTGACTAAGAAGAAAATGGTATTGGAACATTTAGTTGTTGGAAGGTTAAAGGCTCGAAACATCAACCAGGTTGCAAATTTTGAATACATTGACGAAGCAGAGGGCGTAGAAGAGGAGGAACCAAAAAAGGCTGCAGTTGACAGTAGTCCTACTGACAATGATAAATCAAATTTCTGGGAAGATTTGTTAAAGGACAGATATGAAGTGCATAAAGTTGAAGAATTAAACGCTTTAGGCAAAGGGAAGAGAAGTCGTAAGCAGGCCTTGAAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

413

Amino Acids

46.18

Weight (kDa)

8.8

Isoelectric Point (pI)

46.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	147 - 268	4.4e-20	DEAD/DEAH box helicase
DUF1087	PF06465	359 - 410	1.1e-19	CHD subfamily II, DUF1087

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001045)

Species	Orthologous Gene IDs
pyrus_communis	pycom02g04540
rosa_chinensis	RchiOBHm_Chr1g0338921 RchiOBHm_Chr1g0342131 RchiOBHm_Chr2g0159881 RchiOBHm_Chr5g0002061 RchiOBHm_Chr5g0051771 RchiOBHm_Chr5g0076941 RchiOBHm_Chr6g0282081 RchiOBHm_Chr7g0198131
rosa_laevigata	RLG00000003496 RLG00000019204 RLG00000019210 RLG00000031093 RLG00000034339 RLG00000035955
rosa_multiflora	Rmu_co8265413.1_g000001 Rmu_sc0000569.1_g000024 Rmu_sc0000585.1_g000006 Rmu_sc0000780.1_g000015 Rmu_sc0001192.1_g000043 Rmu_sc0001700.1_g000048 Rmu_sc0003092.1_g000032 Rmu_sc0004502.1_g000020 Rmu_sc0004991.1_g000005 Rmu_sc0041726.1_g000001
rosa_roxburghii	Rroxscaffold_1G00033570 Rroxscaffold_1G00035950 Rroxscaffold_1G00047770 Rroxscaffold_2G00124770 Rroxscaffold_2G00143760 Rroxscaffold_3G00227140 Rroxscaffold_5G00341620
rosa_rugosa	Rorug01G0102800 Rorug02G0472100 Rorug03G0011600 Rorug04G0024700 Rorug05G0042700
rosa_samantha	Rh1AG421900 Rh2AG356700 Rh2BG577700 Rh2CG210300 Rh2DG229300 Rh2DG624300 Rh3CG229200 Rh4AG386800 Rh4CG238600 Rh4DG226600 Rh4DG393700 Rh5AG380700 Rh5BG360500 Rh5DG260600 Rh6AG053500 Rh6AG258300 Rh6BG316700 Rh6CG120500 Rh6CG260600 Rh6DG252700 Rh6DG497500 Rh7AG364300 Rh7CG383100
rosa_wichuraiana	Rw6G022360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	247
AccB1I	GGYRCC	1 cut(s)	515
AciI	CCGC	3 cut(s)	392, 580, 584
AcoI	YGGCCR	2 cut(s)	46, 581
AcsI	RAATTY	4 cut(s)	134, 726, 1042, 1135
AcuI	CTGAAG	3 cut(s)	98, 458, 752
AcyI	GRCGYC	1 cut(s)	516
AfaI	GTAC	2 cut(s)	895, 908
AfeI	AGCGCT	1 cut(s)	531
AfiI	CCNNNNNNNGG	2 cut(s)	512, 749
AgsI	TTSAA	7 cut(s)	291, 842, 869, 950, 1049, 1184, 1234
AjnI	CCWGG	3 cut(s)	505, 604, 1032
AjuI	GAANNNNNNNTTGG	2 cut(s)	971, 1003
AluBI	AGCT	7 cut(s)	61, 92, 196, 326, 456, 466, 497
AluI	AGCT	7 cut(s)	61, 92, 196, 326, 456, 466, 497
Alw26I	GTCTC	1 cut(s)	313
Ama87I	CYCGRG	1 cut(s)	451
Aor51HI	AGCGCT	1 cut(s)	531
AoxI	GGCC	4 cut(s)	46, 503, 581, 1227
ApeKI	GCWGC	3 cut(s)	456, 880, 1097
ApoI	RAATTY	4 cut(s)	134, 726, 1042, 1135
AspLEI	GCGC	2 cut(s)	518, 532
AspS9I	GGNCC	1 cut(s)	503
AsuC2I	CCSGG	1 cut(s)	259
AsuHPI	GGTGA	4 cut(s)	199, 710, 764, 925
AvaI	CYCGRG	1 cut(s)	451
BaeI	ACNNNNGTAYC	2 cut(s)	60, 93
BalI	TGGCCA	1 cut(s)	48
BanI	GGYRCC	1 cut(s)	515
BauI	CACGAG	1 cut(s)	936
BbvI	GCAGC	3 cut(s)	443, 892, 1084
BciT130I	CCWGG	3 cut(s)	507, 606, 1034
BclI	TGATCA	1 cut(s)	481
BcnI	CCSGG	1 cut(s)	259
BcoDI	GTCTC	1 cut(s)	313
BfaI	CTAG	4 cut(s)	89, 140, 309, 650
BfmI	CTRYAG	7 cut(s)	357, 425, 457, 525, 861, 875, 1098
BfoI	RGCGCY	2 cut(s)	519, 533
BfuAI	ACCTGC	1 cut(s)	247
BglI	GCCNNNNNGGC	1 cut(s)	1235
BisI	GCNGC	5 cut(s)	457, 581, 584, 881, 1098
BlsI	GCNGC	5 cut(s)	458, 582, 585, 882, 1099
Bme1390I	CCNGG	4 cut(s)	259, 507, 606, 1034
BmeT110I	CYCGRG	1 cut(s)	451
BmgT120I	GGNCC	1 cut(s)	503
BmiI	GGNNCC	3 cut(s)	517, 827, 1086
BmrFI	CCNGG	4 cut(s)	259, 507, 606, 1034
BmsI	GCATC	3 cut(s)	385, 857, 951
BoxI	GACNNNNGTC	1 cut(s)	1110
BpuMI	CCSGG	1 cut(s)	259
BsaHI	GRCGYC	1 cut(s)	516
BsaI	GGTCTC	1 cut(s)	313
BsaJI	CCNNGG	2 cut(s)	200, 408
Bsc4I	CCNNNNNNNGG	2 cut(s)	512, 749
Bse1I	ACTGG	1 cut(s)	899
BseBI	CCWGG	3 cut(s)	507, 606, 1034
BseDI	CCNNGG	2 cut(s)	200, 408
BseGI	GGATG	1 cut(s)	400
BseLI	CCNNNNNNNGG	2 cut(s)	512, 749
BseMII	CTCAG	1 cut(s)	76
BseNI	ACTGG	1 cut(s)	899
BseRI	GAGGAG	3 cut(s)	555, 716, 1094
BseX3I	CGGCCG	1 cut(s)	581
BseXI	GCAGC	3 cut(s)	443, 892, 1084
BsgI	GTGCAG	2 cut(s)	225, 923
Bsh1285I	CGRYCG	1 cut(s)	584
BshFI	GGCC	4 cut(s)	48, 505, 583, 1229
BshNI	GGYRCC	1 cut(s)	515
BsiEI	CGRYCG	1 cut(s)	584
BsiHKCI	CYCGRG	1 cut(s)	451
BsiSI	CCGG	1 cut(s)	258
BslFI	GGGAC	1 cut(s)	433
BslI	CCNNNNNNNGG	2 cut(s)	512, 749
BsmAI	GTCTC	1 cut(s)	313
BsmFI	GGGAC	1 cut(s)	433
BsnI	GGCC	4 cut(s)	48, 505, 583, 1229
Bso31I	GGTCTC	1 cut(s)	313
BsoBI	CYCGRG	1 cut(s)	451
Bsp143I	GATC	3 cut(s)	109, 189, 481
BspACI	CCGC	3 cut(s)	392, 580, 584
BspANI	GGCC	4 cut(s)	48, 505, 583, 1229
BspCNI	CTCAG	1 cut(s)	75
BspLI	GGNNCC	3 cut(s)	517, 827, 1086
BspMAI	CTGCAG	3 cut(s)	429, 461, 1102
BspMI	ACCTGC	1 cut(s)	247
BspT107I	GGYRCC	1 cut(s)	515
BspTNI	GGTCTC	1 cut(s)	313
BsrI	ACTGG	1 cut(s)	899
BssECI	CCNNGG	2 cut(s)	200, 408
BssMI	GATC	3 cut(s)	109, 189, 481
BssNI	GRCGYC	1 cut(s)	516
BssSI	CACGAG	1 cut(s)	936
BssT1I	CCWWGG	2 cut(s)	200, 408
Bst2BI	CACGAG	1 cut(s)	936
Bst2UI	CCWGG	3 cut(s)	507, 606, 1034
Bst4CI	ACNGT	4 cut(s)	361, 402, 865, 1109
Bst6I	CTCTTC	2 cut(s)	1071, 1205
BstACI	GRCGYC	1 cut(s)	516
BstAPI	GCANNNNNTGC	1 cut(s)	239
BstC8I	GCNNGC	2 cut(s)	468, 1227
BstDEI	CTNAG	3 cut(s)	62, 821, 972
BstF5I	GGATG	1 cut(s)	400
BstH2I	RGCGCY	2 cut(s)	519, 533
BstHHI	GCGC	2 cut(s)	518, 532
BstKTI	GATC	3 cut(s)	112, 192, 484
BstMAI	GTCTC	1 cut(s)	313
BstMBI	GATC	3 cut(s)	109, 189, 481
BstMCI	CGRYCG	1 cut(s)	584
BstMWI	GCNNNNNNNGC	4 cut(s)	166, 239, 456, 1235
BstNI	CCWGG	3 cut(s)	507, 606, 1034
BstPAI	GACNNNNGTC	1 cut(s)	1110
BstSCI	CCNGG	4 cut(s)	257, 505, 604, 1032
BstSFI	CTRYAG	7 cut(s)	357, 425, 457, 525, 861, 875, 1098
BstV1I	GCAGC	3 cut(s)	443, 892, 1084
BstXI	CCANNNNNNTGG	1 cut(s)	500
BstZI	CGGCCG	1 cut(s)	581
BsuRI	GGCC	4 cut(s)	48, 505, 583, 1229
BtsCI	GGATG	1 cut(s)	400
BtsI	GCAGTG	1 cut(s)	422
BtsIMutI	CAGTG	5 cut(s)	70, 366, 382, 422, 906
BveI	ACCTGC	1 cut(s)	247
Cac8I	GCNNGC	2 cut(s)	468, 1227
CciNI	GCGGCCGC	1 cut(s)	581
CfoI	GCGC	2 cut(s)	518, 532
Cfr13I	GGNCC	1 cut(s)	503
CseI	GACGC	1 cut(s)	618
CsiI	ACCWGGT	1 cut(s)	1032
Csp6I	GTAC	2 cut(s)	894, 907
CspCI	CAANNNNNGTGG	4 cut(s)	165, 200, 279, 314
CviAII	CATG	2 cut(s)	130, 746
CviQI	GTAC	2 cut(s)	894, 907
DdeI	CTNAG	3 cut(s)	62, 821, 972
DinI	GGCGCC	1 cut(s)	517
DpnI	GATC	3 cut(s)	111, 191, 483
DpnII	GATC	3 cut(s)	109, 189, 481
EaeI	YGGCCR	2 cut(s)	46, 581
EagI	CGGCCG	1 cut(s)	581
Eam1104I	CTCTTC	2 cut(s)	1071, 1205
EarI	CTCTTC	2 cut(s)	1071, 1205
EciI	GGCGGA	1 cut(s)	407
EclXI	CGGCCG	1 cut(s)	581
Eco130I	CCWWGG	2 cut(s)	200, 408
Eco147I	AGGCCT	1 cut(s)	1229
Eco31I	GGTCTC	1 cut(s)	313
Eco47III	AGCGCT	1 cut(s)	531
Eco52I	CGGCCG	1 cut(s)	581
Eco57I	CTGAAG	3 cut(s)	98, 458, 752
Eco88I	CYCGRG	1 cut(s)	451
EcoRII	CCWGG	3 cut(s)	505, 604, 1032
EcoT14I	CCWWGG	2 cut(s)	200, 408
EgeI	GGCGCC	1 cut(s)	517
EheI	GGCGCC	1 cut(s)	517
ErhI	CCWWGG	2 cut(s)	200, 408
FaeI	CATG	2 cut(s)	133, 749
FalI	AAGNNNNNCTT	1 cut(s)	1214
FaqI	GGGAC	1 cut(s)	433
FatI	CATG	2 cut(s)	129, 745
FbaI	TGATCA	1 cut(s)	481
Fnu4HI	GCNGC	5 cut(s)	457, 581, 584, 881, 1098
FokI	GGATG	1 cut(s)	407
Fsp4HI	GCNGC	5 cut(s)	457, 581, 584, 881, 1098
FspBI	CTAG	4 cut(s)	89, 140, 309, 650
GlaI	GCGC	2 cut(s)	517, 531
GluI	GCNGC	5 cut(s)	457, 581, 584, 881, 1098
HaeII	RGCGCY	2 cut(s)	519, 533
HaeIII	GGCC	4 cut(s)	48, 505, 583, 1229
HapII	CCGG	1 cut(s)	258
HgaI	GACGC	1 cut(s)	618
HhaI	GCGC	2 cut(s)	518, 532
Hin1I	GRCGYC	1 cut(s)	516
Hin1II	CATG	2 cut(s)	133, 749
Hin6I	GCGC	2 cut(s)	516, 530
HinP1I	GCGC	2 cut(s)	516, 530
HincII	GTYRAC	2 cut(s)	405, 1105
HindII	GTYRAC	2 cut(s)	405, 1105
HinfI	GANTC	1 cut(s)	642
HpaI	GTTAAC	1 cut(s)	405
HpaII	CCGG	1 cut(s)	258
HphI	GGTGA	4 cut(s)	199, 710, 764, 925
Hpy166II	GTNNAC	4 cut(s)	405, 755, 896, 1105
Hpy188I	TCNGA	3 cut(s)	477, 711, 732
Hpy188III	TCNNGA	3 cut(s)	107, 208, 799
Hpy8I	GTNNAC	4 cut(s)	405, 755, 896, 1105
HpyAV	CCTTC	5 cut(s)	626, 727, 796, 1002, 1228
HpyCH4III	ACNGT	4 cut(s)	361, 402, 865, 1109
HpyF10VI	GCNNNNNNNGC	4 cut(s)	166, 239, 456, 1235
HpyF3I	CTNAG	3 cut(s)	62, 821, 972
Hsp92I	GRCGYC	1 cut(s)	516
Hsp92II	CATG	2 cut(s)	133, 749
HspAI	GCGC	2 cut(s)	516, 530
KasI	GGCGCC	1 cut(s)	515
Ksp22I	TGATCA	1 cut(s)	481
KspAI	GTTAAC	1 cut(s)	405
Kzo9I	GATC	3 cut(s)	109, 189, 481
LmnI	GCTCC	1 cut(s)	463
Lsp1109I	GCAGC	3 cut(s)	443, 892, 1084
LweI	GCATC	3 cut(s)	385, 857, 951
MabI	ACCWGGT	1 cut(s)	1032
MaeI	CTAG	4 cut(s)	89, 140, 309, 650
MaeIII	GTNAC	2 cut(s)	220, 276
MalI	GATC	3 cut(s)	111, 191, 483
MboI	GATC	3 cut(s)	109, 189, 481
MboII	GAAGA	8 cut(s)	104, 847, 962, 988, 1088, 1157, 1196, 1222
MfeI	CAATTG	2 cut(s)	18, 945
MlsI	TGGCCA	1 cut(s)	48
MluNI	TGGCCA	1 cut(s)	48
Mly113I	GGCGCC	1 cut(s)	516
MmeI	TCCRAC	2 cut(s)	517, 985
Mox20I	TGGCCA	1 cut(s)	48
MscI	TGGCCA	1 cut(s)	48
MseI	TTAA	5 cut(s)	404, 660, 1013, 1154, 1190
MslI	CAYNNNNRTG	2 cut(s)	750, 782
Msp20I	TGGCCA	1 cut(s)	48
MspI	CCGG	1 cut(s)	258
MspR9I	CCNGG	4 cut(s)	259, 507, 606, 1034
MunI	CAATTG	2 cut(s)	18, 945
MvaI	CCWGG	3 cut(s)	507, 606, 1034
MwoI	GCNNNNNNNGC	4 cut(s)	166, 239, 456, 1235
NarI	GGCGCC	1 cut(s)	516
NciI	CCSGG	1 cut(s)	259
NdeII	GATC	3 cut(s)	109, 189, 481
NlaIII	CATG	2 cut(s)	133, 749
NlaIV	GGNNCC	3 cut(s)	517, 827, 1086
NmuCI	GTSAC	1 cut(s)	220
NotI	GCGGCCGC	1 cut(s)	581
PaeR7I	CTCGAG	1 cut(s)	451
PceI	AGGCCT	1 cut(s)	1229
PfeI	GAWTC	1 cut(s)	642
PflFI	GACNNNGTC	1 cut(s)	226
PkrI	GCNGC	5 cut(s)	458, 582, 585, 882, 1099
PluTI	GGCGCC	1 cut(s)	519
PshAI	GACNNNNGTC	1 cut(s)	1110
Psp6I	CCWGG	3 cut(s)	505, 604, 1032
PspGI	CCWGG	3 cut(s)	505, 604, 1032
PspN4I	GGNNCC	3 cut(s)	517, 827, 1086
PspPI	GGNCC	1 cut(s)	503
PspXI	VCTCGAGB	1 cut(s)	451
PstI	CTGCAG	3 cut(s)	429, 461, 1102
PsyI	GACNNNGTC	1 cut(s)	226
RsaI	GTAC	2 cut(s)	895, 908
RsaNI	GTAC	2 cut(s)	894, 907
RseI	CAYNNNNRTG	2 cut(s)	750, 782
SaqAI	TTAA	5 cut(s)	404, 660, 1013, 1154, 1190
SatI	GCNGC	5 cut(s)	457, 581, 584, 881, 1098
Sau3AI	GATC	3 cut(s)	109, 189, 481
Sau96I	GGNCC	1 cut(s)	503
ScrFI	CCNGG	4 cut(s)	259, 507, 606, 1034
SexAI	ACCWGGT	1 cut(s)	1032
SfaNI	GCATC	3 cut(s)	385, 857, 951
SfcI	CTRYAG	7 cut(s)	357, 425, 457, 525, 861, 875, 1098
SfoI	GGCGCC	1 cut(s)	517
Sfr274I	CTCGAG	1 cut(s)	451
SlaI	CTCGAG	1 cut(s)	451
SmiMI	CAYNNNNRTG	2 cut(s)	750, 782
SmlI	CTYRAG	1 cut(s)	451
SmoI	CTYRAG	1 cut(s)	451
SseBI	AGGCCT	1 cut(s)	1229
SsiI	CCGC	3 cut(s)	392, 580, 584
SspDI	GGCGCC	1 cut(s)	515
SspI	AATATT	2 cut(s)	295, 665
SspMI	CTAG	4 cut(s)	89, 140, 309, 650
StuI	AGGCCT	1 cut(s)	1229
StyD4I	CCNGG	4 cut(s)	257, 505, 604, 1032
StyI	CCWWGG	2 cut(s)	200, 408
TaaI	ACNGT	4 cut(s)	361, 402, 865, 1109
TaqI	TCGA	4 cut(s)	192, 452, 851, 1021
TatI	WGTACW	2 cut(s)	893, 906
TauI	GCSGC	2 cut(s)	583, 586
TfiI	GAWTC	1 cut(s)	642
Tru1I	TTAA	5 cut(s)	404, 660, 1013, 1154, 1190
Tru9I	TTAA	5 cut(s)	404, 660, 1013, 1154, 1190
TscAI	CASTG	5 cut(s)	70, 366, 382, 429, 906
TseFI	GTSAC	1 cut(s)	220
TseI	GCWGC	3 cut(s)	456, 880, 1097
Tsp45I	GTSAC	1 cut(s)	220
TspDTI	ATGAA	4 cut(s)	146, 608, 738, 1182
TspGWI	ACGGA	1 cut(s)	772
TspRI	CASTG	5 cut(s)	70, 366, 382, 429, 906
Tth111I	GACNNNGTC	1 cut(s)	226
XapI	RAATTY	4 cut(s)	134, 726, 1042, 1135
XhoI	CTCGAG	1 cut(s)	451
XspI	CTAG	4 cut(s)	89, 140, 309, 650

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.