NADP-dependent alkenal double bond reductase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 108, 520, 587 |
| AclWI | GGATC | 1 cut(s) | 40 |
| AcuI | CTGAAG | 2 cut(s) | 290, 672 |
| AfaI | GTAC | 4 cut(s) | 12, 461, 486, 614 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 146, 282 |
| AgsI | TTSAA | 2 cut(s) | 43, 527 |
| AjnI | CCWGG | 1 cut(s) | 97 |
| AluBI | AGCT | 4 cut(s) | 170, 193, 629, 656 |
| AluI | AGCT | 4 cut(s) | 170, 193, 629, 656 |
| Alw26I | GTCTC | 1 cut(s) | 146 |
| AlwI | GGATC | 1 cut(s) | 40 |
| Ama87I | CYCGRG | 1 cut(s) | 638 |
| ApeKI | GCWGC | 2 cut(s) | 170, 329 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 353, 385 |
| AspLEI | GCGC | 1 cut(s) | 334 |
| AspS9I | GGNCC | 1 cut(s) | 647 |
| AvaI | CYCGRG | 1 cut(s) | 638 |
| AvaII | GGWCC | 1 cut(s) | 647 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 415, 448 |
| BanII | GRGCYC | 1 cut(s) | 639 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 596, 628 |
| BauI | CACGAG | 3 cut(s) | 32, 659, 711 |
| BbsI | GAAGAC | 1 cut(s) | 278 |
| BbvI | GCAGC | 2 cut(s) | 157, 316 |
| BccI | CCATC | 2 cut(s) | 211, 626 |
| BciT130I | CCWGG | 1 cut(s) | 99 |
| BcoDI | GTCTC | 1 cut(s) | 146 |
| BfaI | CTAG | 1 cut(s) | 395 |
| BglII | AGATCT | 1 cut(s) | 129 |
| BisI | GCNGC | 2 cut(s) | 171, 330 |
| BlsI | GCNGC | 2 cut(s) | 172, 331 |
| Bme1390I | CCNGG | 1 cut(s) | 99 |
| Bme18I | GGWCC | 1 cut(s) | 647 |
| BmeT110I | CYCGRG | 1 cut(s) | 638 |
| BmgT120I | GGNCC | 1 cut(s) | 647 |
| BmrFI | CCNGG | 1 cut(s) | 99 |
| BmsI | GCATC | 3 cut(s) | 182, 388, 592 |
| BpiI | GAAGAC | 1 cut(s) | 278 |
| BpuEI | CTTGAG | 1 cut(s) | 488 |
| BsaAI | YACGTR | 1 cut(s) | 616 |
| BsaJI | CCNNGG | 1 cut(s) | 673 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 141, 171 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 146, 282 |
| Bse1I | ACTGG | 1 cut(s) | 85 |
| BseBI | CCWGG | 1 cut(s) | 99 |
| BseDI | CCNNGG | 1 cut(s) | 673 |
| BseGI | GGATG | 1 cut(s) | 583 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 146, 282 |
| BseNI | ACTGG | 1 cut(s) | 85 |
| BseRI | GAGGAG | 1 cut(s) | 21 |
| BseXI | GCAGC | 2 cut(s) | 157, 316 |
| BsiHKCI | CYCGRG | 1 cut(s) | 638 |
| BsiWI | CGTACG | 1 cut(s) | 612 |
| BslFI | GGGAC | 2 cut(s) | 213, 678 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 146, 282 |
| BsmAI | GTCTC | 1 cut(s) | 146 |
| BsmFI | GGGAC | 2 cut(s) | 213, 678 |
| BsmI | GAATGC | 1 cut(s) | 522 |
| BsoBI | CYCGRG | 1 cut(s) | 638 |
| Bsp1286I | GDGCHC | 1 cut(s) | 639 |
| Bsp1407I | TGTACA | 1 cut(s) | 484 |
| Bsp143I | GATC | 4 cut(s) | 45, 129, 262, 450 |
| Bsp19I | CCATGG | 1 cut(s) | 673 |
| BspACI | CCGC | 3 cut(s) | 108, 520, 587 |
| BspPI | GGATC | 1 cut(s) | 40 |
| BsrGI | TGTACA | 1 cut(s) | 484 |
| BsrI | ACTGG | 1 cut(s) | 85 |
| BssECI | CCNNGG | 1 cut(s) | 673 |
| BssMI | GATC | 4 cut(s) | 45, 129, 262, 450 |
| BssSI | CACGAG | 3 cut(s) | 32, 659, 711 |
| BssT1I | CCWWGG | 1 cut(s) | 673 |
| Bst2BI | CACGAG | 3 cut(s) | 32, 659, 711 |
| Bst2UI | CCWGG | 1 cut(s) | 99 |
| Bst4CI | ACNGT | 3 cut(s) | 15, 157, 459 |
| Bst6I | CTCTTC | 1 cut(s) | 674 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 179 |
| BstAUI | TGTACA | 1 cut(s) | 484 |
| BstBAI | YACGTR | 1 cut(s) | 616 |
| BstDEI | CTNAG | 2 cut(s) | 210, 323 |
| BstDSI | CCRYGG | 1 cut(s) | 673 |
| BstF5I | GGATG | 1 cut(s) | 583 |
| BstHHI | GCGC | 1 cut(s) | 334 |
| BstKTI | GATC | 4 cut(s) | 48, 132, 265, 453 |
| BstMAI | GTCTC | 1 cut(s) | 146 |
| BstMBI | GATC | 4 cut(s) | 45, 129, 262, 450 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 179, 528 |
| BstNI | CCWGG | 1 cut(s) | 99 |
| BstNSI | RCATGY | 1 cut(s) | 424 |
| BstSCI | CCNGG | 1 cut(s) | 97 |
| BstV1I | GCAGC | 2 cut(s) | 157, 316 |
| BstV2I | GAAGAC | 1 cut(s) | 278 |
| BstX2I | RGATCY | 2 cut(s) | 45, 129 |
| BstYI | RGATCY | 2 cut(s) | 45, 129 |
| BtgI | CCRYGG | 1 cut(s) | 673 |
| BtsCI | GGATG | 1 cut(s) | 583 |
| BtsI | GCAGTG | 1 cut(s) | 408 |
| BtsIMutI | CAGTG | 3 cut(s) | 20, 78, 408 |
| CfoI | GCGC | 1 cut(s) | 334 |
| Cfr13I | GGNCC | 1 cut(s) | 647 |
| Csp6I | GTAC | 4 cut(s) | 11, 460, 485, 613 |
| CviAII | CATG | 3 cut(s) | 415, 421, 674 |
| CviJI | RGCY | 7 cut(s) | 170, 193, 222, 531, 629, 637, 656 |
| CviKI_1 | RGCY | 7 cut(s) | 170, 193, 222, 531, 629, 637, 656 |
| CviQI | GTAC | 4 cut(s) | 11, 460, 485, 613 |
| DdeI | CTNAG | 2 cut(s) | 210, 323 |
| DpnI | GATC | 4 cut(s) | 47, 131, 264, 452 |
| DpnII | GATC | 4 cut(s) | 45, 129, 262, 450 |
| Eam1104I | CTCTTC | 1 cut(s) | 674 |
| EarI | CTCTTC | 1 cut(s) | 674 |
| Eco130I | CCWWGG | 1 cut(s) | 673 |
| Eco24I | GRGCYC | 1 cut(s) | 639 |
| Eco47I | GGWCC | 1 cut(s) | 647 |
| Eco57I | CTGAAG | 2 cut(s) | 290, 672 |
| Eco88I | CYCGRG | 1 cut(s) | 638 |
| EcoO109I | RGGNCCY | 1 cut(s) | 647 |
| EcoRII | CCWGG | 1 cut(s) | 97 |
| EcoT14I | CCWWGG | 1 cut(s) | 673 |
| EcoT22I | ATGCAT | 1 cut(s) | 298 |
| EcoT38I | GRGCYC | 1 cut(s) | 639 |
| ErhI | CCWWGG | 1 cut(s) | 673 |
| FaeI | CATG | 3 cut(s) | 418, 424, 677 |
| FaqI | GGGAC | 2 cut(s) | 213, 678 |
| FatI | CATG | 3 cut(s) | 414, 420, 673 |
| FauNDI | CATATG | 1 cut(s) | 112 |
| Fnu4HI | GCNGC | 2 cut(s) | 171, 330 |
| FokI | GGATG | 1 cut(s) | 570 |
| FriOI | GRGCYC | 1 cut(s) | 639 |
| Fsp4HI | GCNGC | 2 cut(s) | 171, 330 |
| FspBI | CTAG | 1 cut(s) | 395 |
| GlaI | GCGC | 1 cut(s) | 333 |
| GluI | GCNGC | 2 cut(s) | 171, 330 |
| HhaI | GCGC | 1 cut(s) | 334 |
| Hin1II | CATG | 3 cut(s) | 418, 424, 677 |
| Hin6I | GCGC | 1 cut(s) | 332 |
| HinP1I | GCGC | 1 cut(s) | 332 |
| HinfI | GANTC | 3 cut(s) | 28, 516, 666 |
| Hpy166II | GTNNAC | 2 cut(s) | 485, 487 |
| Hpy188I | TCNGA | 1 cut(s) | 544 |
| Hpy188III | TCNNGA | 7 cut(s) | 32, 43, 268, 467, 640, 650, 713 |
| Hpy8I | GTNNAC | 2 cut(s) | 485, 487 |
| Hpy99I | CGWCG | 1 cut(s) | 557 |
| HpyAV | CCTTC | 2 cut(s) | 473, 593 |
| HpyCH4III | ACNGT | 3 cut(s) | 15, 157, 459 |
| HpyCH4IV | ACGT | 2 cut(s) | 611, 615 |
| HpyCH4V | TGCA | 5 cut(s) | 173, 296, 401, 424, 583 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 179, 528 |
| HpyF3I | CTNAG | 2 cut(s) | 210, 323 |
| HpySE526I | ACGT | 2 cut(s) | 611, 615 |
| Hsp92II | CATG | 3 cut(s) | 418, 424, 677 |
| HspAI | GCGC | 1 cut(s) | 332 |
| Kzo9I | GATC | 4 cut(s) | 45, 129, 262, 450 |
| LpnPI | CCDG | 8 cut(s) | 66, 84, 111, 162, 228, 281, 330, 663 |
| Lsp1109I | GCAGC | 2 cut(s) | 157, 316 |
| LweI | GCATC | 3 cut(s) | 182, 388, 592 |
| MaeI | CTAG | 1 cut(s) | 395 |
| MaeII | ACGT | 2 cut(s) | 611, 615 |
| MalI | GATC | 4 cut(s) | 47, 131, 264, 452 |
| MboI | GATC | 4 cut(s) | 45, 129, 262, 450 |
| MboII | GAAGA | 5 cut(s) | 283, 323, 616, 632, 661 |
| MflI | RGATCY | 2 cut(s) | 45, 129 |
| MhlI | GDGCHC | 1 cut(s) | 639 |
| MluCI | AATT | 1 cut(s) | 203 |
| MlyI | GAGTC | 2 cut(s) | 22, 660 |
| MmeI | TCCRAC | 3 cut(s) | 73, 214, 522 |
| MnlI | CCTC | 1 cut(s) | 377 |
| Mph1103I | ATGCAT | 1 cut(s) | 298 |
| MseI | TTAA | 2 cut(s) | 300, 594 |
| MslI | CAYNNNNRTG | 2 cut(s) | 57, 419 |
| MspA1I | CMGCKG | 1 cut(s) | 170 |
| MspR9I | CCNGG | 1 cut(s) | 99 |
| Mva1269I | GAATGC | 1 cut(s) | 522 |
| MvaI | CCWGG | 1 cut(s) | 99 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 179, 528 |
| NcoI | CCATGG | 1 cut(s) | 673 |
| NdeI | CATATG | 1 cut(s) | 112 |
| NdeII | GATC | 4 cut(s) | 45, 129, 262, 450 |
| NlaIII | CATG | 3 cut(s) | 418, 424, 677 |
| NsiI | ATGCAT | 1 cut(s) | 298 |
| NspI | RCATGY | 1 cut(s) | 424 |
| PaeR7I | CTCGAG | 1 cut(s) | 638 |
| PctI | GAATGC | 1 cut(s) | 522 |
| PfeI | GAWTC | 1 cut(s) | 516 |
| Pfl23II | CGTACG | 1 cut(s) | 612 |
| PkrI | GCNGC | 2 cut(s) | 172, 331 |
| PleI | GAGTC | 2 cut(s) | 22, 660 |
| PpsI | GAGTC | 2 cut(s) | 22, 660 |
| Ppu21I | YACGTR | 1 cut(s) | 616 |
| PpuMI | RGGWCCY | 1 cut(s) | 647 |
| Psp5II | RGGWCCY | 1 cut(s) | 647 |
| Psp6I | CCWGG | 1 cut(s) | 97 |
| PspGI | CCWGG | 1 cut(s) | 97 |
| PspLI | CGTACG | 1 cut(s) | 612 |
| PspPI | GGNCC | 1 cut(s) | 647 |
| PspPPI | RGGWCCY | 1 cut(s) | 647 |
| PsuI | RGATCY | 2 cut(s) | 45, 129 |
| PvuII | CAGCTG | 1 cut(s) | 170 |
| RsaI | GTAC | 4 cut(s) | 12, 461, 486, 614 |
| RsaNI | GTAC | 4 cut(s) | 11, 460, 485, 613 |
| RseI | CAYNNNNRTG | 2 cut(s) | 57, 419 |
| SaqAI | TTAA | 2 cut(s) | 300, 594 |
| SatI | GCNGC | 2 cut(s) | 171, 330 |
| Sau3AI | GATC | 4 cut(s) | 45, 129, 262, 450 |
| Sau96I | GGNCC | 1 cut(s) | 647 |
| SchI | GAGTC | 2 cut(s) | 22, 660 |
| ScrFI | CCNGG | 1 cut(s) | 99 |
| SduI | GDGCHC | 1 cut(s) | 639 |
| SfaNI | GCATC | 3 cut(s) | 182, 388, 592 |
| Sfr274I | CTCGAG | 1 cut(s) | 638 |
| SinI | GGWCC | 1 cut(s) | 647 |
| SlaI | CTCGAG | 1 cut(s) | 638 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 57, 419 |
| SmlI | CTYRAG | 2 cut(s) | 467, 638 |
| SmoI | CTYRAG | 2 cut(s) | 467, 638 |
| Sse9I | AATT | 1 cut(s) | 203 |
| SsiI | CCGC | 3 cut(s) | 108, 520, 587 |
| SspMI | CTAG | 1 cut(s) | 395 |
| StyD4I | CCNGG | 1 cut(s) | 97 |
| StyI | CCWWGG | 1 cut(s) | 673 |
| TaaI | ACNGT | 3 cut(s) | 15, 157, 459 |
| TaiI | ACGT | 2 cut(s) | 614, 618 |
| TaqI | TCGA | 1 cut(s) | 639 |
| TasI | AATT | 1 cut(s) | 203 |
| TatI | WGTACW | 3 cut(s) | 10, 459, 484 |
| TfiI | GAWTC | 1 cut(s) | 516 |
| Tru1I | TTAA | 2 cut(s) | 300, 594 |
| Tru9I | TTAA | 2 cut(s) | 300, 594 |
| TscAI | CASTG | 3 cut(s) | 20, 85, 408 |
| TseI | GCWGC | 2 cut(s) | 170, 329 |
| TspDTI | ATGAA | 2 cut(s) | 425, 431 |
| TspRI | CASTG | 3 cut(s) | 20, 85, 408 |
| VpaK11BI | GGWCC | 1 cut(s) | 647 |
| XceI | RCATGY | 1 cut(s) | 424 |
| XhoI | CTCGAG | 1 cut(s) | 638 |
| XspI | CTAG | 1 cut(s) | 395 |
| Zsp2I | ATGCAT | 1 cut(s) | 298 |