NADP-dependent alkenal double bond reductase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 648 |
| AccB1I | GGYRCC | 1 cut(s) | 294 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 260 |
| AccII | CGCG | 1 cut(s) | 1056 |
| AciI | CCGC | 3 cut(s) | 140, 184, 560 |
| AclWI | GGATC | 5 cut(s) | 126, 139, 167, 200, 669 |
| AcoI | YGGCCR | 1 cut(s) | 769 |
| AcsI | RAATTY | 1 cut(s) | 887 |
| AfaI | GTAC | 1 cut(s) | 123 |
| AfeI | AGCGCT | 1 cut(s) | 583 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 260, 488, 681 |
| AflIII | ACRYGT | 2 cut(s) | 118, 931 |
| AgsI | TTSAA | 3 cut(s) | 385, 643, 869 |
| AjnI | CCWGG | 1 cut(s) | 216 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 322, 354, 358, 390 |
| AluBI | AGCT | 2 cut(s) | 512, 881 |
| AluI | AGCT | 2 cut(s) | 512, 881 |
| Alw21I | GWGCWC | 1 cut(s) | 750 |
| Alw26I | GTCTC | 2 cut(s) | 449, 488 |
| AlwI | GGATC | 5 cut(s) | 126, 139, 167, 200, 669 |
| Aor51HI | AGCGCT | 1 cut(s) | 583 |
| AoxI | GGCC | 1 cut(s) | 769 |
| ApeKI | GCWGC | 4 cut(s) | 512, 671, 779, 878 |
| ApoI | RAATTY | 1 cut(s) | 887 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 695, 727 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 793 |
| AspLEI | GCGC | 2 cut(s) | 524, 584 |
| AspS9I | GGNCC | 1 cut(s) | 989 |
| AsuC2I | CCSGG | 2 cut(s) | 129, 441 |
| AsuHPI | GGTGA | 1 cut(s) | 55 |
| AsuNHI | GCTAGC | 1 cut(s) | 584 |
| AvaII | GGWCC | 1 cut(s) | 989 |
| BaeGI | GKGCMC | 1 cut(s) | 297 |
| BamHI | GGATCC | 1 cut(s) | 131 |
| BanI | GGYRCC | 1 cut(s) | 294 |
| Bbv12I | GWGCWC | 1 cut(s) | 750 |
| BbvI | GCAGC | 4 cut(s) | 499, 658, 791, 890 |
| BccI | CCATC | 1 cut(s) | 550 |
| BceAI | ACGGC | 1 cut(s) | 42 |
| BciT130I | CCWGG | 1 cut(s) | 218 |
| BcnI | CCSGG | 2 cut(s) | 129, 441 |
| BcoDI | GTCTC | 2 cut(s) | 449, 488 |
| BfaI | CTAG | 2 cut(s) | 146, 585 |
| BfoI | RGCGCY | 1 cut(s) | 585 |
| BisI | GCNGC | 5 cut(s) | 184, 513, 672, 780, 879 |
| BlsI | GCNGC | 5 cut(s) | 185, 514, 673, 781, 880 |
| Bme1390I | CCNGG | 3 cut(s) | 129, 218, 441 |
| Bme18I | GGWCC | 1 cut(s) | 989 |
| BmgT120I | GGNCC | 1 cut(s) | 989 |
| BmiI | GGNNCC | 3 cut(s) | 133, 296, 816 |
| BmrFI | CCNGG | 3 cut(s) | 129, 218, 441 |
| BmsI | GCATC | 3 cut(s) | 524, 554, 867 |
| BmtI | GCTAGC | 1 cut(s) | 588 |
| BpuEI | CTTGAG | 2 cut(s) | 596, 1001 |
| BpuMI | CCSGG | 2 cut(s) | 129, 441 |
| BsaAI | YACGTR | 3 cut(s) | 61, 119, 934 |
| BsaI | GGTCTC | 1 cut(s) | 449 |
| BsaJI | CCNNGG | 5 cut(s) | 135, 216, 225, 973, 1015 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 352, 382, 483, 513 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 260, 488, 681 |
| Bse1I | ACTGG | 1 cut(s) | 427 |
| Bse3DI | GCAATG | 1 cut(s) | 93 |
| BseBI | CCWGG | 1 cut(s) | 218 |
| BseDI | CCNNGG | 5 cut(s) | 135, 216, 225, 973, 1015 |
| BseGI | GGATG | 3 cut(s) | 212, 569, 858 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 260, 488, 681 |
| BseMI | GCAATG | 1 cut(s) | 93 |
| BseNI | ACTGG | 1 cut(s) | 427 |
| BseSI | GKGCMC | 1 cut(s) | 297 |
| BseXI | GCAGC | 4 cut(s) | 499, 658, 791, 890 |
| Bsh1236I | CGCG | 1 cut(s) | 1056 |
| BshFI | GGCC | 1 cut(s) | 771 |
| BshNI | GGYRCC | 1 cut(s) | 294 |
| BsiHKAI | GWGCWC | 1 cut(s) | 750 |
| BsiSI | CCGG | 2 cut(s) | 129, 440 |
| BslFI | GGGAC | 1 cut(s) | 555 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 260, 488, 681 |
| BsmAI | GTCTC | 2 cut(s) | 449, 488 |
| BsmFI | GGGAC | 1 cut(s) | 555 |
| BsnI | GGCC | 1 cut(s) | 771 |
| Bso31I | GGTCTC | 1 cut(s) | 449 |
| Bsp1286I | GDGCHC | 2 cut(s) | 297, 750 |
| Bsp143I | GATC | 6 cut(s) | 131, 172, 205, 604, 661, 1002 |
| Bsp19I | CCATGG | 2 cut(s) | 135, 225 |
| Bsp68I | TCGCGA | 1 cut(s) | 1056 |
| BspACI | CCGC | 3 cut(s) | 140, 184, 560 |
| BspANI | GGCC | 1 cut(s) | 771 |
| BspFNI | CGCG | 1 cut(s) | 1056 |
| BspLI | GGNNCC | 3 cut(s) | 133, 296, 816 |
| BspOI | GCTAGC | 1 cut(s) | 588 |
| BspPI | GGATC | 5 cut(s) | 126, 139, 167, 200, 669 |
| BspT107I | GGYRCC | 1 cut(s) | 294 |
| BspTNI | GGTCTC | 1 cut(s) | 449 |
| BsrDI | GCAATG | 1 cut(s) | 93 |
| BsrI | ACTGG | 1 cut(s) | 427 |
| BssECI | CCNNGG | 5 cut(s) | 135, 216, 225, 973, 1015 |
| BssMI | GATC | 6 cut(s) | 131, 172, 205, 604, 661, 1002 |
| BssT1I | CCWWGG | 3 cut(s) | 135, 225, 973 |
| Bst2UI | CCWGG | 1 cut(s) | 218 |
| Bst4CI | ACNGT | 3 cut(s) | 499, 546, 1019 |
| Bst6I | CTCTTC | 3 cut(s) | 325, 339, 1016 |
| BstBAI | YACGTR | 3 cut(s) | 61, 119, 934 |
| BstC8I | GCNNGC | 3 cut(s) | 251, 586, 1054 |
| BstDEI | CTNAG | 2 cut(s) | 618, 665 |
| BstDSI | CCRYGG | 3 cut(s) | 135, 225, 1015 |
| BstF5I | GGATG | 3 cut(s) | 212, 569, 858 |
| BstFNI | CGCG | 1 cut(s) | 1056 |
| BstH2I | RGCGCY | 1 cut(s) | 585 |
| BstHHI | GCGC | 2 cut(s) | 524, 584 |
| BstKTI | GATC | 6 cut(s) | 134, 175, 208, 607, 664, 1005 |
| BstMAI | GTCTC | 2 cut(s) | 449, 488 |
| BstMBI | GATC | 6 cut(s) | 131, 172, 205, 604, 661, 1002 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 26, 189, 227, 521, 557 |
| BstNI | CCWGG | 1 cut(s) | 218 |
| BstSCI | CCNGG | 3 cut(s) | 127, 216, 439 |
| BstSLI | GKGCMC | 1 cut(s) | 297 |
| BstUI | CGCG | 1 cut(s) | 1056 |
| BstV1I | GCAGC | 4 cut(s) | 499, 658, 791, 890 |
| BstX2I | RGATCY | 2 cut(s) | 131, 661 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 913 |
| BstYI | RGATCY | 2 cut(s) | 131, 661 |
| BsuRI | GGCC | 1 cut(s) | 771 |
| BtgI | CCRYGG | 3 cut(s) | 135, 225, 1015 |
| BtsCI | GGATG | 3 cut(s) | 212, 569, 858 |
| BtsI | GCAGTG | 2 cut(s) | 447, 750 |
| BtsIMutI | CAGTG | 3 cut(s) | 420, 447, 750 |
| BtuMI | TCGCGA | 1 cut(s) | 1056 |
| Cac8I | GCNNGC | 3 cut(s) | 251, 586, 1054 |
| CfoI | GCGC | 2 cut(s) | 524, 584 |
| Cfr13I | GGNCC | 1 cut(s) | 989 |
| CseI | GACGC | 2 cut(s) | 644, 749 |
| Csp6I | GTAC | 1 cut(s) | 122 |
| CviAII | CATG | 6 cut(s) | 136, 193, 226, 233, 757, 767 |
| CviQI | GTAC | 1 cut(s) | 122 |
| DdeI | CTNAG | 2 cut(s) | 618, 665 |
| DpnI | GATC | 6 cut(s) | 133, 174, 207, 606, 663, 1004 |
| DpnII | GATC | 6 cut(s) | 131, 172, 205, 604, 661, 1002 |
| EaeI | YGGCCR | 1 cut(s) | 769 |
| Eam1104I | CTCTTC | 3 cut(s) | 325, 339, 1016 |
| EarI | CTCTTC | 3 cut(s) | 325, 339, 1016 |
| Eco130I | CCWWGG | 3 cut(s) | 135, 225, 973 |
| Eco31I | GGTCTC | 1 cut(s) | 449 |
| Eco47I | GGWCC | 1 cut(s) | 989 |
| Eco47III | AGCGCT | 1 cut(s) | 583 |
| EcoRII | CCWGG | 1 cut(s) | 216 |
| EcoT14I | CCWWGG | 3 cut(s) | 135, 225, 973 |
| ErhI | CCWWGG | 3 cut(s) | 135, 225, 973 |
| FaeI | CATG | 6 cut(s) | 139, 196, 229, 236, 760, 770 |
| FaqI | GGGAC | 1 cut(s) | 555 |
| FatI | CATG | 6 cut(s) | 135, 192, 225, 232, 756, 766 |
| FauI | CCCGC | 1 cut(s) | 553 |
| FauNDI | CATATG | 1 cut(s) | 955 |
| Fnu4HI | GCNGC | 5 cut(s) | 184, 513, 672, 780, 879 |
| FokI | GGATG | 3 cut(s) | 199, 576, 845 |
| Fsp4HI | GCNGC | 5 cut(s) | 184, 513, 672, 780, 879 |
| FspBI | CTAG | 2 cut(s) | 146, 585 |
| GlaI | GCGC | 2 cut(s) | 523, 583 |
| GluI | GCNGC | 5 cut(s) | 184, 513, 672, 780, 879 |
| HaeII | RGCGCY | 1 cut(s) | 585 |
| HaeIII | GGCC | 1 cut(s) | 771 |
| HapII | CCGG | 2 cut(s) | 129, 440 |
| HgaI | GACGC | 2 cut(s) | 644, 749 |
| HhaI | GCGC | 2 cut(s) | 524, 584 |
| Hin1II | CATG | 6 cut(s) | 139, 196, 229, 236, 760, 770 |
| Hin6I | GCGC | 2 cut(s) | 522, 582 |
| HinP1I | GCGC | 2 cut(s) | 522, 582 |
| HincII | GTYRAC | 1 cut(s) | 533 |
| HindII | GTYRAC | 1 cut(s) | 533 |
| HinfI | GANTC | 7 cut(s) | 46, 69, 154, 287, 388, 774, 1008 |
| HpaII | CCGG | 2 cut(s) | 129, 440 |
| HphI | GGTGA | 1 cut(s) | 55 |
| Hpy166II | GTNNAC | 4 cut(s) | 122, 533, 827, 850 |
| Hpy188I | TCNGA | 3 cut(s) | 85, 483, 1007 |
| Hpy188III | TCNNGA | 6 cut(s) | 151, 385, 613, 869, 1030, 1055 |
| Hpy8I | GTNNAC | 4 cut(s) | 122, 533, 827, 850 |
| Hpy99I | CGWCG | 3 cut(s) | 25, 28, 1031 |
| HpyAV | CCTTC | 6 cut(s) | 381, 592, 689, 815, 935, 1002 |
| HpyCH4III | ACNGT | 3 cut(s) | 499, 546, 1019 |
| HpyCH4IV | ACGT | 5 cut(s) | 60, 118, 720, 933, 1026 |
| HpyCH4V | TGCA | 3 cut(s) | 15, 515, 878 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 26, 189, 227, 521, 557 |
| HpyF3I | CTNAG | 2 cut(s) | 618, 665 |
| HpySE526I | ACGT | 5 cut(s) | 60, 118, 720, 933, 1026 |
| Hsp92II | CATG | 6 cut(s) | 139, 196, 229, 236, 760, 770 |
| HspAI | GCGC | 2 cut(s) | 522, 582 |
| Kzo9I | GATC | 6 cut(s) | 131, 172, 205, 604, 661, 1002 |
| LmnI | GCTCC | 1 cut(s) | 753 |
| Lsp1109I | GCAGC | 4 cut(s) | 499, 658, 791, 890 |
| LweI | GCATC | 3 cut(s) | 524, 554, 867 |
| MaeI | CTAG | 2 cut(s) | 146, 585 |
| MaeII | ACGT | 5 cut(s) | 60, 118, 720, 933, 1026 |
| MaeIII | GTNAC | 1 cut(s) | 53 |
| MalI | GATC | 6 cut(s) | 133, 174, 207, 606, 663, 1004 |
| MboI | GATC | 6 cut(s) | 131, 172, 205, 604, 661, 1002 |
| MboII | GAAGA | 9 cut(s) | 342, 356, 366, 496, 565, 599, 665, 958, 1003 |
| MflI | RGATCY | 2 cut(s) | 131, 661 |
| MhlI | GDGCHC | 2 cut(s) | 297, 750 |
| MluCI | AATT | 3 cut(s) | 301, 643, 887 |
| MlyI | GAGTC | 1 cut(s) | 1002 |
| MmeI | TCCRAC | 3 cut(s) | 108, 389, 556 |
| MnlI | CCTC | 8 cut(s) | 98, 135, 186, 220, 675, 719, 753, 955 |
| MroXI | GAANNNNTTC | 1 cut(s) | 793 |
| MslI | CAYNNNNRTG | 2 cut(s) | 191, 399 |
| MspA1I | CMGCKG | 2 cut(s) | 512, 881 |
| MspI | CCGG | 2 cut(s) | 129, 440 |
| MspR9I | CCNGG | 3 cut(s) | 129, 218, 441 |
| MvaI | CCWGG | 1 cut(s) | 218 |
| MvnI | CGCG | 1 cut(s) | 1056 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 26, 189, 227, 521, 557 |
| NciI | CCSGG | 2 cut(s) | 129, 441 |
| NcoI | CCATGG | 2 cut(s) | 135, 225 |
| NdeI | CATATG | 1 cut(s) | 955 |
| NdeII | GATC | 6 cut(s) | 131, 172, 205, 604, 661, 1002 |
| NheI | GCTAGC | 1 cut(s) | 584 |
| NlaIII | CATG | 6 cut(s) | 139, 196, 229, 236, 760, 770 |
| NlaIV | GGNNCC | 3 cut(s) | 133, 296, 816 |
| NmuCI | GTSAC | 1 cut(s) | 53 |
| NruI | TCGCGA | 1 cut(s) | 1056 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1023 |
| PdmI | GAANNNNTTC | 1 cut(s) | 793 |
| PfeI | GAWTC | 6 cut(s) | 46, 69, 154, 287, 388, 774 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 260 |
| PfoI | TCCNGGA | 1 cut(s) | 127 |
| PkrI | GCNGC | 5 cut(s) | 185, 514, 673, 781, 880 |
| PleI | GAGTC | 1 cut(s) | 1002 |
| PpsI | GAGTC | 1 cut(s) | 1002 |
| Ppu21I | YACGTR | 3 cut(s) | 61, 119, 934 |
| PsiI | TTATAA | 1 cut(s) | 648 |
| Psp6I | CCWGG | 1 cut(s) | 216 |
| PspGI | CCWGG | 1 cut(s) | 216 |
| PspN4I | GGNNCC | 3 cut(s) | 133, 296, 816 |
| PspPI | GGNCC | 1 cut(s) | 989 |
| PsuI | RGATCY | 2 cut(s) | 131, 661 |
| PvuII | CAGCTG | 2 cut(s) | 512, 881 |
| RruI | TCGCGA | 1 cut(s) | 1056 |
| RsaI | GTAC | 1 cut(s) | 123 |
| RsaNI | GTAC | 1 cut(s) | 122 |
| RseI | CAYNNNNRTG | 2 cut(s) | 191, 399 |
| SatI | GCNGC | 5 cut(s) | 184, 513, 672, 780, 879 |
| Sau3AI | GATC | 6 cut(s) | 131, 172, 205, 604, 661, 1002 |
| Sau96I | GGNCC | 1 cut(s) | 989 |
| SchI | GAGTC | 1 cut(s) | 1002 |
| ScrFI | CCNGG | 3 cut(s) | 129, 218, 441 |
| SduI | GDGCHC | 2 cut(s) | 297, 750 |
| SfaNI | GCATC | 3 cut(s) | 524, 554, 867 |
| SinI | GGWCC | 1 cut(s) | 989 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 191, 399 |
| SmlI | CTYRAG | 2 cut(s) | 611, 980 |
| SmoI | CTYRAG | 2 cut(s) | 611, 980 |
| Sse9I | AATT | 3 cut(s) | 301, 643, 887 |
| SsiI | CCGC | 3 cut(s) | 140, 184, 560 |
| SspMI | CTAG | 2 cut(s) | 146, 585 |
| StyD4I | CCNGG | 3 cut(s) | 127, 216, 439 |
| StyI | CCWWGG | 3 cut(s) | 135, 225, 973 |
| TaaI | ACNGT | 3 cut(s) | 499, 546, 1019 |
| TaiI | ACGT | 5 cut(s) | 63, 121, 723, 936, 1029 |
| TaqI | TCGA | 2 cut(s) | 885, 1044 |
| TasI | AATT | 3 cut(s) | 301, 643, 887 |
| TauI | GCSGC | 1 cut(s) | 186 |
| TfiI | GAWTC | 6 cut(s) | 46, 69, 154, 287, 388, 774 |
| TscAI | CASTG | 3 cut(s) | 427, 454, 750 |
| TseFI | GTSAC | 1 cut(s) | 53 |
| TseI | GCWGC | 4 cut(s) | 512, 671, 779, 878 |
| Tsp45I | GTSAC | 1 cut(s) | 53 |
| TspDTI | ATGAA | 2 cut(s) | 380, 910 |
| TspGWI | ACGGA | 2 cut(s) | 41, 851 |
| TspRI | CASTG | 3 cut(s) | 427, 454, 750 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 260 |
| VpaK11BI | GGWCC | 1 cut(s) | 989 |
| XapI | RAATTY | 1 cut(s) | 887 |
| XmnI | GAANNNNTTC | 1 cut(s) | 793 |
| XspI | CTAG | 2 cut(s) | 146, 585 |