2-alkenal reductase (NADP( )-dependent)-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 102 |
| Acc36I | ACCTGC | 1 cut(s) | 1216 |
| AciI | CCGC | 3 cut(s) | 636, 1053, 1152 |
| AclWI | GGATC | 1 cut(s) | 859 |
| AcoI | YGGCCR | 4 cut(s) | 28, 35, 242, 950 |
| AcsI | RAATTY | 5 cut(s) | 122, 154, 307, 314, 401 |
| AcuI | CTGAAG | 2 cut(s) | 414, 1403 |
| AcyI | GRCGYC | 1 cut(s) | 99 |
| AdeI | CACNNNGTG | 1 cut(s) | 1085 |
| AfaI | GTAC | 1 cut(s) | 1365 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 369 |
| AflII | CTTAAG | 1 cut(s) | 167 |
| AhlI | ACTAGT | 1 cut(s) | 1217 |
| AjnI | CCWGG | 2 cut(s) | 69, 1081 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 31, 63 |
| AluBI | AGCT | 8 cut(s) | 172, 389, 932, 1061, 1283, 1370, 1475, 1549 |
| AluI | AGCT | 8 cut(s) | 172, 389, 932, 1061, 1283, 1370, 1475, 1549 |
| Alw26I | GTCTC | 3 cut(s) | 90, 829, 1303 |
| AlwI | GGATC | 1 cut(s) | 859 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1061 |
| AoxI | GGCC | 8 cut(s) | 28, 35, 242, 371, 950, 1238, 1439, 1494 |
| ApeKI | GCWGC | 3 cut(s) | 781, 1061, 1283 |
| ApoI | RAATTY | 5 cut(s) | 122, 154, 307, 314, 401 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 509, 541 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 739 |
| AspS9I | GGNCC | 4 cut(s) | 372, 894, 1239, 1494 |
| AsuC2I | CCSGG | 1 cut(s) | 17 |
| AsuHPI | GGTGA | 4 cut(s) | 290, 666, 998, 1071 |
| AvaII | GGWCC | 1 cut(s) | 894 |
| BalI | TGGCCA | 2 cut(s) | 244, 952 |
| BbsI | GAAGAC | 1 cut(s) | 500 |
| BbvI | GCAGC | 3 cut(s) | 793, 1048, 1270 |
| BccI | CCATC | 5 cut(s) | 373, 626, 826, 875, 1474 |
| BceAI | ACGGC | 3 cut(s) | 15, 43, 1272 |
| BciT130I | CCWGG | 2 cut(s) | 71, 1083 |
| BcnI | CCSGG | 1 cut(s) | 17 |
| BcoDI | GTCTC | 3 cut(s) | 90, 829, 1303 |
| BcuI | ACTAGT | 1 cut(s) | 1217 |
| BfaI | CTAG | 6 cut(s) | 93, 287, 648, 933, 1218, 1371 |
| BfrI | CTTAAG | 1 cut(s) | 167 |
| BfuAI | ACCTGC | 1 cut(s) | 1216 |
| BisI | GCNGC | 4 cut(s) | 782, 1053, 1062, 1284 |
| BlsI | GCNGC | 4 cut(s) | 783, 1054, 1063, 1285 |
| Bme1390I | CCNGG | 3 cut(s) | 17, 71, 1083 |
| Bme18I | GGWCC | 1 cut(s) | 894 |
| BmgT120I | GGNCC | 4 cut(s) | 372, 894, 1239, 1494 |
| BmiI | GGNNCC | 2 cut(s) | 1260, 1333 |
| BmrFI | CCNGG | 3 cut(s) | 17, 71, 1083 |
| BmsI | GCATC | 4 cut(s) | 282, 599, 979, 1404 |
| BoxI | GACNNNNGTC | 1 cut(s) | 30 |
| BpiI | GAAGAC | 1 cut(s) | 500 |
| BpuMI | CCSGG | 1 cut(s) | 17 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1272 |
| BsaHI | GRCGYC | 1 cut(s) | 99 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 369 |
| Bse1I | ACTGG | 2 cut(s) | 511, 1198 |
| Bse3DI | GCAATG | 3 cut(s) | 220, 580, 1017 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1272 |
| BseBI | CCWGG | 2 cut(s) | 71, 1083 |
| BseGI | GGATG | 2 cut(s) | 88, 886 |
| BseJI | GATNNNNATC | 1 cut(s) | 1272 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 369 |
| BseMI | GCAATG | 3 cut(s) | 220, 580, 1017 |
| BseMII | CTCAG | 5 cut(s) | 645, 669, 813, 939, 1314 |
| BseNI | ACTGG | 2 cut(s) | 511, 1198 |
| BseRI | GAGGAG | 1 cut(s) | 1107 |
| BseX3I | CGGCCG | 1 cut(s) | 28 |
| BseXI | GCAGC | 3 cut(s) | 793, 1048, 1270 |
| BseYI | CCCAGC | 2 cut(s) | 434, 1491 |
| BsgI | GTGCAG | 1 cut(s) | 1244 |
| Bsh1285I | CGRYCG | 1 cut(s) | 31 |
| BshFI | GGCC | 8 cut(s) | 30, 37, 244, 373, 952, 1240, 1441, 1496 |
| BsiEI | CGRYCG | 1 cut(s) | 31 |
| BsiSI | CCGG | 1 cut(s) | 16 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 369 |
| BsmAI | GTCTC | 3 cut(s) | 90, 829, 1303 |
| BsmBI | CGTCTC | 2 cut(s) | 90, 1303 |
| BsmI | GAATGC | 2 cut(s) | 111, 1154 |
| BsnI | GGCC | 8 cut(s) | 30, 37, 244, 373, 952, 1240, 1441, 1496 |
| Bsp143I | GATC | 1 cut(s) | 864 |
| BspACI | CCGC | 3 cut(s) | 636, 1053, 1152 |
| BspANI | GGCC | 8 cut(s) | 30, 37, 244, 373, 952, 1240, 1441, 1496 |
| BspCNI | CTCAG | 5 cut(s) | 646, 670, 814, 938, 1313 |
| BspHI | TCATGA | 2 cut(s) | 916, 1276 |
| BspLI | GGNNCC | 2 cut(s) | 1260, 1333 |
| BspMI | ACCTGC | 1 cut(s) | 1216 |
| BspPI | GGATC | 1 cut(s) | 859 |
| BspTI | CTTAAG | 1 cut(s) | 167 |
| BsrDI | GCAATG | 3 cut(s) | 220, 580, 1017 |
| BsrI | ACTGG | 2 cut(s) | 511, 1198 |
| BssMI | GATC | 1 cut(s) | 864 |
| BssNI | GRCGYC | 1 cut(s) | 99 |
| Bst2UI | CCWGG | 2 cut(s) | 71, 1083 |
| Bst4CI | ACNGT | 2 cut(s) | 468, 862 |
| Bst6I | CTCTTC | 2 cut(s) | 1084, 1249 |
| BstACI | GRCGYC | 1 cut(s) | 99 |
| BstAFI | CTTAAG | 1 cut(s) | 167 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1061 |
| BstC8I | GCNNGC | 1 cut(s) | 855 |
| BstDEI | CTNAG | 6 cut(s) | 269, 654, 678, 822, 925, 1300 |
| BstF5I | GGATG | 2 cut(s) | 88, 886 |
| BstKTI | GATC | 1 cut(s) | 867 |
| BstMAI | GTCTC | 3 cut(s) | 90, 829, 1303 |
| BstMBI | GATC | 1 cut(s) | 864 |
| BstMCI | CGRYCG | 1 cut(s) | 31 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 648, 1058, 1061, 1376 |
| BstNI | CCWGG | 2 cut(s) | 71, 1083 |
| BstNSI | RCATGY | 2 cut(s) | 996, 1364 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 30 |
| BstSCI | CCNGG | 3 cut(s) | 15, 69, 1081 |
| BstV1I | GCAGC | 3 cut(s) | 793, 1048, 1270 |
| BstV2I | GAAGAC | 1 cut(s) | 500 |
| BstZI | CGGCCG | 1 cut(s) | 28 |
| BsuRI | GGCC | 8 cut(s) | 30, 37, 244, 373, 952, 1240, 1441, 1496 |
| BtsCI | GGATG | 2 cut(s) | 88, 886 |
| BtsIMutI | CAGTG | 2 cut(s) | 149, 651 |
| BveI | ACCTGC | 1 cut(s) | 1216 |
| Cac8I | GCNNGC | 1 cut(s) | 855 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1061 |
| CciI | TCATGA | 2 cut(s) | 916, 1276 |
| Cfr13I | GGNCC | 4 cut(s) | 372, 894, 1239, 1494 |
| CsiI | ACCWGGT | 1 cut(s) | 1081 |
| Csp6I | GTAC | 1 cut(s) | 1364 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 334, 369, 1486, 1521 |
| CviAII | CATG | 5 cut(s) | 778, 917, 993, 1277, 1361 |
| CviQI | GTAC | 1 cut(s) | 1364 |
| DdeI | CTNAG | 6 cut(s) | 269, 654, 678, 822, 925, 1300 |
| DpnI | GATC | 1 cut(s) | 866 |
| DpnII | GATC | 1 cut(s) | 864 |
| DraI | TTTAAA | 1 cut(s) | 666 |
| DraIII | CACNNNGTG | 1 cut(s) | 1085 |
| EaeI | YGGCCR | 4 cut(s) | 28, 35, 242, 950 |
| EagI | CGGCCG | 1 cut(s) | 28 |
| Eam1104I | CTCTTC | 2 cut(s) | 1084, 1249 |
| EarI | CTCTTC | 2 cut(s) | 1084, 1249 |
| EciI | GGCGGA | 1 cut(s) | 651 |
| EclXI | CGGCCG | 1 cut(s) | 28 |
| Eco47I | GGWCC | 1 cut(s) | 894 |
| Eco52I | CGGCCG | 1 cut(s) | 28 |
| Eco57I | CTGAAG | 2 cut(s) | 414, 1403 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1239 |
| EcoRI | GAATTC | 3 cut(s) | 307, 314, 401 |
| EcoRII | CCWGG | 2 cut(s) | 69, 1081 |
| EcoT22I | ATGCAT | 1 cut(s) | 994 |
| Esp3I | CGTCTC | 2 cut(s) | 90, 1303 |
| FaeI | CATG | 5 cut(s) | 781, 920, 996, 1280, 1364 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1228, 1260, 1401, 1433 |
| FatI | CATG | 5 cut(s) | 777, 916, 992, 1276, 1360 |
| FauNDI | CATATG | 1 cut(s) | 1341 |
| Fnu4HI | GCNGC | 4 cut(s) | 782, 1053, 1062, 1284 |
| FokI | GGATG | 2 cut(s) | 95, 893 |
| Fsp4HI | GCNGC | 4 cut(s) | 782, 1053, 1062, 1284 |
| FspBI | CTAG | 6 cut(s) | 93, 287, 648, 933, 1218, 1371 |
| GluI | GCNGC | 4 cut(s) | 782, 1053, 1062, 1284 |
| GsaI | CCCAGC | 2 cut(s) | 438, 1495 |
| HaeIII | GGCC | 8 cut(s) | 30, 37, 244, 373, 952, 1240, 1441, 1496 |
| HapII | CCGG | 1 cut(s) | 16 |
| Hin1I | GRCGYC | 1 cut(s) | 99 |
| Hin1II | CATG | 5 cut(s) | 781, 920, 996, 1280, 1364 |
| HindIII | AAGCTT | 1 cut(s) | 170 |
| HinfI | GANTC | 8 cut(s) | 55, 556, 575, 936, 943, 1142, 1306, 1524 |
| HpaII | CCGG | 1 cut(s) | 16 |
| HphI | GGTGA | 4 cut(s) | 290, 666, 998, 1071 |
| Hpy188III | TCNNGA | 6 cut(s) | 93, 392, 917, 940, 1187, 1277 |
| Hpy99I | CGWCG | 2 cut(s) | 29, 36 |
| HpyAV | CCTTC | 3 cut(s) | 296, 878, 1389 |
| HpyCH4III | ACNGT | 2 cut(s) | 468, 862 |
| HpyCH4IV | ACGT | 2 cut(s) | 99, 321 |
| HpyCH4V | TGCA | 9 cut(s) | 590, 781, 992, 1017, 1022, 1064, 1225, 1360, 1539 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 648, 1058, 1061, 1376 |
| HpyF3I | CTNAG | 6 cut(s) | 269, 654, 678, 822, 925, 1300 |
| HpySE526I | ACGT | 2 cut(s) | 99, 321 |
| Hsp92I | GRCGYC | 1 cut(s) | 99 |
| Hsp92II | CATG | 5 cut(s) | 781, 920, 996, 1280, 1364 |
| Kzo9I | GATC | 1 cut(s) | 864 |
| LmnI | GCTCC | 3 cut(s) | 394, 1094, 1264 |
| Lsp1109I | GCAGC | 3 cut(s) | 793, 1048, 1270 |
| LweI | GCATC | 4 cut(s) | 282, 599, 979, 1404 |
| MabI | ACCWGGT | 1 cut(s) | 1081 |
| MaeI | CTAG | 6 cut(s) | 93, 287, 648, 933, 1218, 1371 |
| MaeII | ACGT | 2 cut(s) | 99, 321 |
| MaeIII | GTNAC | 5 cut(s) | 202, 1077, 1228, 1321, 1423 |
| MalI | GATC | 1 cut(s) | 866 |
| MboI | GATC | 1 cut(s) | 864 |
| MboII | GAAGA | 9 cut(s) | 395, 407, 505, 735, 897, 1101, 1236, 1411, 1476 |
| MfeI | CAATTG | 1 cut(s) | 902 |
| MlsI | TGGCCA | 2 cut(s) | 244, 952 |
| MluNI | TGGCCA | 2 cut(s) | 244, 952 |
| MlyI | GAGTC | 3 cut(s) | 49, 952, 1533 |
| MmeI | TCCRAC | 2 cut(s) | 77, 1507 |
| MnlI | CCTC | 7 cut(s) | 415, 632, 651, 1085, 1252, 1345, 1501 |
| Mox20I | TGGCCA | 2 cut(s) | 244, 952 |
| Mph1103I | ATGCAT | 1 cut(s) | 994 |
| MroXI | GAANNNNTTC | 1 cut(s) | 739 |
| MscI | TGGCCA | 2 cut(s) | 244, 952 |
| MseI | TTAA | 7 cut(s) | 168, 665, 725, 1007, 1011, 1461, 1520 |
| MslI | CAYNNNNRTG | 1 cut(s) | 466 |
| Msp20I | TGGCCA | 2 cut(s) | 244, 952 |
| MspCI | CTTAAG | 1 cut(s) | 167 |
| MspI | CCGG | 1 cut(s) | 16 |
| MspR9I | CCNGG | 3 cut(s) | 17, 71, 1083 |
| MunI | CAATTG | 1 cut(s) | 902 |
| Mva1269I | GAATGC | 2 cut(s) | 111, 1154 |
| MvaI | CCWGG | 2 cut(s) | 71, 1083 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 648, 1058, 1061, 1376 |
| NciI | CCSGG | 1 cut(s) | 17 |
| NdeI | CATATG | 1 cut(s) | 1341 |
| NdeII | GATC | 1 cut(s) | 864 |
| NlaIII | CATG | 5 cut(s) | 781, 920, 996, 1280, 1364 |
| NlaIV | GGNNCC | 2 cut(s) | 1260, 1333 |
| NmuCI | GTSAC | 4 cut(s) | 202, 1077, 1321, 1423 |
| NsiI | ATGCAT | 1 cut(s) | 994 |
| NspI | RCATGY | 2 cut(s) | 996, 1364 |
| PacI | TTAATTAA | 1 cut(s) | 1011 |
| PagI | TCATGA | 2 cut(s) | 916, 1276 |
| PctI | GAATGC | 2 cut(s) | 111, 1154 |
| PdmI | GAANNNNTTC | 1 cut(s) | 739 |
| PfeI | GAWTC | 5 cut(s) | 556, 575, 936, 1142, 1306 |
| PkrI | GCNGC | 4 cut(s) | 783, 1054, 1063, 1285 |
| PleI | GAGTC | 3 cut(s) | 49, 951, 1532 |
| PpsI | GAGTC | 3 cut(s) | 49, 951, 1532 |
| PshAI | GACNNNNGTC | 1 cut(s) | 30 |
| Psp6I | CCWGG | 2 cut(s) | 69, 1081 |
| PspFI | CCCAGC | 2 cut(s) | 434, 1491 |
| PspGI | CCWGG | 2 cut(s) | 69, 1081 |
| PspN4I | GGNNCC | 2 cut(s) | 1260, 1333 |
| PspPI | GGNCC | 4 cut(s) | 372, 894, 1239, 1494 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1061 |
| RsaI | GTAC | 1 cut(s) | 1365 |
| RsaNI | GTAC | 1 cut(s) | 1364 |
| RseI | CAYNNNNRTG | 1 cut(s) | 466 |
| SaqAI | TTAA | 7 cut(s) | 168, 665, 725, 1007, 1011, 1461, 1520 |
| SatI | GCNGC | 4 cut(s) | 782, 1053, 1062, 1284 |
| Sau3AI | GATC | 1 cut(s) | 864 |
| Sau96I | GGNCC | 4 cut(s) | 372, 894, 1239, 1494 |
| SchI | GAGTC | 3 cut(s) | 49, 952, 1533 |
| ScrFI | CCNGG | 3 cut(s) | 17, 71, 1083 |
| SexAI | ACCWGGT | 1 cut(s) | 1081 |
| SfaNI | GCATC | 4 cut(s) | 282, 599, 979, 1404 |
| SinI | GGWCC | 1 cut(s) | 894 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 466 |
| SmlI | CTYRAG | 1 cut(s) | 167 |
| SmoI | CTYRAG | 1 cut(s) | 167 |
| SpeI | ACTAGT | 1 cut(s) | 1217 |
| SsiI | CCGC | 3 cut(s) | 636, 1053, 1152 |
| SspI | AATATT | 2 cut(s) | 739, 1514 |
| SspMI | CTAG | 6 cut(s) | 93, 287, 648, 933, 1218, 1371 |
| StyD4I | CCNGG | 3 cut(s) | 15, 69, 1081 |
| TaaI | ACNGT | 2 cut(s) | 468, 862 |
| TaiI | ACGT | 2 cut(s) | 102, 324 |
| TauI | GCSGC | 1 cut(s) | 1055 |
| TfiI | GAWTC | 5 cut(s) | 556, 575, 936, 1142, 1306 |
| Tru1I | TTAA | 7 cut(s) | 168, 665, 725, 1007, 1011, 1461, 1520 |
| Tru9I | TTAA | 7 cut(s) | 168, 665, 725, 1007, 1011, 1461, 1520 |
| TscAI | CASTG | 2 cut(s) | 149, 658 |
| TseFI | GTSAC | 4 cut(s) | 202, 1077, 1321, 1423 |
| TseI | GCWGC | 3 cut(s) | 781, 1061, 1283 |
| Tsp45I | GTSAC | 4 cut(s) | 202, 1077, 1321, 1423 |
| TspGWI | ACGGA | 1 cut(s) | 886 |
| TspRI | CASTG | 2 cut(s) | 149, 658 |
| Vha464I | CTTAAG | 1 cut(s) | 167 |
| VpaK11BI | GGWCC | 1 cut(s) | 894 |
| XapI | RAATTY | 5 cut(s) | 122, 154, 307, 314, 401 |
| XbaI | TCTAGA | 1 cut(s) | 92 |
| XceI | RCATGY | 2 cut(s) | 996, 1364 |
| XmnI | GAANNNNTTC | 1 cut(s) | 739 |
| XspI | CTAG | 6 cut(s) | 93, 287, 648, 933, 1218, 1371 |
| ZraI | GACGTC | 1 cut(s) | 100 |
| Zsp2I | ATGCAT | 1 cut(s) | 994 |