MD16G1282000.v1.1

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000431
Physical Location & Seq
Forward (+)
38435239 .. 38436459
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1282000.v1.1.491

Sequence Viewer

Length: 489 bp
ATGTGCATGCCGGGTATAACTGCCTTCGTTGGTTTTCATGAAAACTGTTCGCCGAAGAAAGGAGATTATGTCTTCGTTTCTGCCGCAGCTGGTGCAGTTGGTCAGCTTGTTGGGCAGTTTGCAAAGCTAATGGGATGCTATGTTGTTGGAAGTGTTGGAAGTAAAGAGAAGGTCGATCTACTAAAGAACAAGCTTGGATTTGATGAGGCTTTCAATTATAAGGAAGAGCCTGACCTGGAGGCAGCTTTGAAAAGGTACTTCCCTGAAGGCATTGACATTTACTTTGAGAACGTTGGAGGAAAAATGCTGGATGCTGTGCTCATTAACATGGGACGTCATGGCCGAATTGCGGTATACGGATTGATCTCATATTACAATCTCAAACAGCCGCAAGCAACCTCGTCTTTATCATCTACAATCGGATTCGCATCGAAGGCTTCGTCGTGTTTGATTATTTCCACCTCTACCCCAAGTTTCTGGACATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

163

Amino Acids

17.58

Weight (kDa)

7.62

Isoelectric Point (pI)

28.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 32 - 124 3.3e-21 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26320 AT1G26320 AT3G03080 AT3G59845 AT3G59845 AT5G16960 AT5G16960 AT5G16970 AT5G16980 AT5G16980 AT5G16980 AT5G16990 AT5G17000 AT5G17000 AT5G37940 AT5G37960 AT5G37980 AT5G38000 AT5G38000 AT5G38000
fragaria_vesca FvH4_1g00340 FvH4_1g18790 FvH4_1g23120 FvH4_1g23120
malus_domestica MD00G1045700.v1.1 MD01G1046700.v1.1 MD02G1163900.v1.1 MD15G1145600.v1.1 MD16G1282000.v1.1
prunus_persica Prupe.1G543700_v2.0.a1 Prupe.4G262000_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.6G211500_v2.0.a1 Prupe.7G269100_v2.0.a1
pyrus_communis pycom02g00040 pycom02g13040 pycom11g16640 pycom12561g00320 pycom15g13080 pycom16g25160
rosa_chinensis RchiOBHm_Chr2g0084641 RchiOBHm_Chr2g0084711 RchiOBHm_Chr2g0085361 RchiOBHm_Chr2g0085371 RchiOBHm_Chr2g0109651 RchiOBHm_Chr2g0117841 RchiOBHm_Chr6g0275751
rosa_laevigata RLG00000013436 RLG00000015629 RLG00000015633 RLG00000015680 RLG00000015681 RLG00000017753 RLG00000018376
rosa_multiflora Rmu_sc0000332.1_g000060 Rmu_sc0002352.1_g000008 Rmu_sc0002352.1_g000012 Rmu_sc0010860.1_g000005 Rmu_sc0012119.1_g000008
rosa_roxburghii Rroxscaffold_2G00126190 Rroxscaffold_2G00134060 Rroxscaffold_2G00155420 Rroxscaffold_2G00155430 Rroxscaffold_2G00155880 Rroxscaffold_2G00155890
rosa_rugosa Rorug01G0455500 Rorug01G0455600 Rorug01G0459700 Rorug01G0459800 Rorug01G0459800 Rorug01G0459900 Rorug02G0161600 Rorug02G0214400
rosa_samantha Rh2BG008800 Rh2BG282100 Rh2CG003900 Rh2CG004600 Rh2CG009500 Rh2CG009600 Rh2CG216100 Rh2CG216200 Rh2CG269100 Rh2CG269200 Rh2DG003700 Rh2DG004100 Rh2DG009900 Rh2DG219300 Rh2DG278400
rosa_wichuraiana Rw2G000300 Rw2G000330 Rw2G000770 Rw2G000780 Rw2G021390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 219
AatII GACGTC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 354
AciI CCGC 3 cut(s) 84, 350, 389
AclI AACGTT 1 cut(s) 291
AcoI YGGCCR 1 cut(s) 340
AcuI CTGAAG 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 334
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 2 cut(s) 59, 349
AgsI TTSAA 2 cut(s) 214, 250
AjnI CCWGG 1 cut(s) 234
AluBI AGCT 5 cut(s) 89, 106, 127, 193, 245
AluI AGCT 5 cut(s) 89, 106, 127, 193, 245
Alw21I GWGCWC 1 cut(s) 321
AoxI GGCC 1 cut(s) 340
ApeKI GCWGC 2 cut(s) 86, 242
ArsI GACNNNNNNTTYG 4 cut(s) 266, 298, 425, 457
AsuC2I CCSGG 1 cut(s) 12
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 98, 254
BciT130I CCWGG 1 cut(s) 236
BcnI CCSGG 1 cut(s) 12
BisI GCNGC 4 cut(s) 84, 87, 243, 389
BlsI GCNGC 4 cut(s) 85, 88, 244, 390
Bme1390I CCNGG 2 cut(s) 12, 236
BmrFI CCNGG 2 cut(s) 12, 236
BmsI GCATC 3 cut(s) 125, 301, 437
BpiI GAAGAC 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 12
BsaBI GATNNNNATC 1 cut(s) 427
BsaHI GRCGYC 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 349
Bse8I GATNNNNATC 1 cut(s) 427
BseBI CCWGG 1 cut(s) 236
BseGI GGATG 2 cut(s) 140, 316
BseJI GATNNNNATC 1 cut(s) 427
BseLI CCNNNNNNNGG 2 cut(s) 59, 349
BseXI GCAGC 2 cut(s) 98, 254
BsgI GTGCAG 1 cut(s) 114
BshFI GGCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 345
BslI CCNNNNNNNGG 2 cut(s) 59, 349
BsmFI GGGAC 1 cut(s) 345
BsnI GGCC 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 2 cut(s) 175, 363
BspACI CCGC 3 cut(s) 84, 350, 389
BspANI GGCC 1 cut(s) 342
BspHI TCATGA 1 cut(s) 37
BspQI GCTCTTC 1 cut(s) 219
BssMI GATC 2 cut(s) 175, 363
BssNAI GTATAC 1 cut(s) 355
BssNI GRCGYC 1 cut(s) 334
Bst1107I GTATAC 1 cut(s) 355
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 1 cut(s) 47
Bst6I CTCTTC 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 334
BstAPI GCANNNNNTGC 1 cut(s) 92
BstC8I GCNNGC 2 cut(s) 8, 393
BstF5I GGATG 2 cut(s) 140, 316
BstKTI GATC 2 cut(s) 178, 366
BstMBI GATC 2 cut(s) 175, 363
BstMWI GCNNNNNNNGC 3 cut(s) 92, 112, 434
BstNI CCWGG 1 cut(s) 236
BstNSI RCATGY 1 cut(s) 10
BstSCI CCNGG 2 cut(s) 10, 234
BstV1I GCAGC 2 cut(s) 98, 254
BstV2I GAAGAC 1 cut(s) 64
BstXI CCANNNNNNTGG 1 cut(s) 477
BstZ17I GTATAC 1 cut(s) 355
BsuRI GGCC 1 cut(s) 342
BtsCI GGATG 2 cut(s) 140, 316
Cac8I GCNNGC 2 cut(s) 8, 393
CciI TCATGA 1 cut(s) 37
Csp6I GTAC 1 cut(s) 256
CviAII CATG 5 cut(s) 7, 38, 328, 338, 483
CviQI GTAC 1 cut(s) 256
DpnI GATC 2 cut(s) 177, 365
DpnII GATC 2 cut(s) 175, 363
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 285
EcoRII CCWGG 1 cut(s) 234
FaeI CATG 5 cut(s) 10, 41, 331, 341, 486
FaqI GGGAC 1 cut(s) 345
FatI CATG 5 cut(s) 6, 37, 327, 337, 482
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 4 cut(s) 84, 87, 243, 389
FokI GGATG 2 cut(s) 147, 323
Fsp4HI GCNGC 4 cut(s) 84, 87, 243, 389
GluI GCNGC 4 cut(s) 84, 87, 243, 389
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 1 cut(s) 342
HapII CCGG 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 334
Hin1II CATG 5 cut(s) 10, 41, 331, 341, 486
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 1 cut(s) 423
HpaII CCGG 1 cut(s) 11
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 1 cut(s) 422
Hpy188III TCNNGA 2 cut(s) 38, 478
Hpy8I GTNNAC 1 cut(s) 355
Hpy99I CGWCG 1 cut(s) 445
HpyAV CCTTC 4 cut(s) 34, 163, 260, 427
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4IV ACGT 2 cut(s) 291, 334
HpyCH4V TGCA 3 cut(s) 6, 95, 122
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 112, 434
HpySE526I ACGT 2 cut(s) 291, 334
Hsp92I GRCGYC 1 cut(s) 334
Hsp92II CATG 5 cut(s) 10, 41, 331, 341, 486
Kzo9I GATC 2 cut(s) 175, 363
LguI GCTCTTC 1 cut(s) 219
LpnPI CCDG 8 cut(s) 24, 75, 221, 243, 248, 276, 293, 463
Lsp1109I GCAGC 2 cut(s) 98, 254
LweI GCATC 3 cut(s) 125, 301, 437
MaeII ACGT 2 cut(s) 291, 334
MalI GATC 2 cut(s) 177, 365
MboI GATC 2 cut(s) 175, 363
MboII GAAGA 3 cut(s) 64, 67, 236
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 2 cut(s) 214, 345
MmeI TCCRAC 3 cut(s) 127, 136, 274
MnlI CCTC 5 cut(s) 199, 232, 290, 409, 472
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 2 cut(s) 12, 236
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 3 cut(s) 92, 112, 434
NciI CCSGG 1 cut(s) 12
NdeII GATC 2 cut(s) 175, 363
NlaIII CATG 5 cut(s) 10, 41, 331, 341, 486
NspI RCATGY 1 cut(s) 10
PaeI GCATGC 1 cut(s) 10
PagI TCATGA 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 219
PcsI WCGNNNNNNNCGW 2 cut(s) 340, 437
PfeI GAWTC 1 cut(s) 423
PkrI GCNGC 4 cut(s) 85, 88, 244, 390
PsiI TTATAA 1 cut(s) 219
Psp1406I AACGTT 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 4 cut(s) 84, 87, 243, 389
Sau3AI GATC 2 cut(s) 175, 363
ScrFI CCNGG 2 cut(s) 12, 236
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 3 cut(s) 125, 301, 437
SmiMI CAYNNNNRTG 1 cut(s) 326
SphI GCATGC 1 cut(s) 10
Sse9I AATT 2 cut(s) 214, 345
SsiI CCGC 3 cut(s) 84, 350, 389
StyD4I CCNGG 2 cut(s) 10, 234
TaaI ACNGT 1 cut(s) 47
TaiI ACGT 2 cut(s) 294, 337
TaqI TCGA 2 cut(s) 174, 431
TasI AATT 2 cut(s) 214, 345
TauI GCSGC 2 cut(s) 86, 391
TfiI GAWTC 1 cut(s) 423
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TseI GCWGC 2 cut(s) 86, 242
TspDTI ATGAA 2 cut(s) 26, 54
TspGWI ACGGA 1 cut(s) 372
XceI RCATGY 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 354
ZraI GACGTC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.