Rroxscaffold_2G00155880

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000431
Physical Location & Seq
Reverse (-)
92363331 .. 92364064
734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155880.1

Sequence Viewer

Length: 516 bp
ATGGCTGGTAAGACTGCTTATGCTGGATTCTTTGAAGTATGTCCTCCTAAGAAAGGAGACAAGGTGTTGGTCTCTGCAGCATCTGGTGCAGTTGGTCAGCTTCTCGGCCAATTCGCTAAGTTGGCTGGCTGTTATGTTGTTGGATCTGCCGGATCTAAACAAAAGGTTGATCTACTGAAGAACAAGTTGCGGTCTGATGAGGCTTTCAATTACAAGGAGGAGAACGATTTAGAAGCAGCTTTGAAAAGGTACTTTCCTGATGGAATTGACTTCTACTTTGAGCATGTGGGTGGGAAATTGTTTGACGCAGTGCTGCTCAACATGAAAGAACACGGTCGCATTGCTGTGTGCGGAATGATTTCACAATACAACCTTCCCGAGCCTGAGCCACTCAAAAACTTGTTCCAGATTGCATTGAAGCGGCTCAATATACATGGATTTACGCATTCCAATTACGATCACATAGTACCCAAGTACTATGAGTTTGTGCTGCCTTACATCCGGAAGGTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

171

Amino Acids

19.14

Weight (kDa)

8.99

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 30 - 158 5.5e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26320 AT1G26320 AT3G03080 AT3G59845 AT3G59845 AT5G16960 AT5G16960 AT5G16970 AT5G16980 AT5G16980 AT5G16980 AT5G16990 AT5G17000 AT5G17000 AT5G37940 AT5G37960 AT5G37980 AT5G38000 AT5G38000 AT5G38000
fragaria_vesca FvH4_1g00340 FvH4_1g18790 FvH4_1g23120 FvH4_1g23120
malus_domestica MD00G1045700.v1.1 MD01G1046700.v1.1 MD02G1163900.v1.1 MD15G1145600.v1.1 MD16G1282000.v1.1
prunus_persica Prupe.1G543700_v2.0.a1 Prupe.4G262000_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.6G211500_v2.0.a1 Prupe.7G269100_v2.0.a1
pyrus_communis pycom02g00040 pycom02g13040 pycom11g16640 pycom12561g00320 pycom15g13080 pycom16g25160
rosa_chinensis RchiOBHm_Chr2g0084641 RchiOBHm_Chr2g0084711 RchiOBHm_Chr2g0085361 RchiOBHm_Chr2g0085371 RchiOBHm_Chr2g0109651 RchiOBHm_Chr2g0117841 RchiOBHm_Chr6g0275751
rosa_laevigata RLG00000013436 RLG00000015629 RLG00000015633 RLG00000015680 RLG00000015681 RLG00000017753 RLG00000018376
rosa_multiflora Rmu_sc0000332.1_g000060 Rmu_sc0002352.1_g000008 Rmu_sc0002352.1_g000012 Rmu_sc0010860.1_g000005 Rmu_sc0012119.1_g000008
rosa_roxburghii Rroxscaffold_2G00126190 Rroxscaffold_2G00134060 Rroxscaffold_2G00155420 Rroxscaffold_2G00155430 Rroxscaffold_2G00155880 Rroxscaffold_2G00155890
rosa_rugosa Rorug01G0455500 Rorug01G0455600 Rorug01G0459700 Rorug01G0459800 Rorug01G0459800 Rorug01G0459900 Rorug02G0161600 Rorug02G0214400
rosa_samantha Rh2BG008800 Rh2BG282100 Rh2CG003900 Rh2CG004600 Rh2CG009500 Rh2CG009600 Rh2CG216100 Rh2CG216200 Rh2CG269100 Rh2CG269200 Rh2DG003700 Rh2DG004100 Rh2DG009900 Rh2DG219300 Rh2DG278400
rosa_wichuraiana Rw2G000300 Rw2G000330 Rw2G000770 Rw2G000780 Rw2G021390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 501
AciI CCGC 3 cut(s) 190, 351, 421
AclWI GGATC 2 cut(s) 151, 160
AcoI YGGCCR 1 cut(s) 106
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 3 cut(s) 251, 468, 476
AfiI CCNNNNNNNGG 1 cut(s) 53
AgsI TTSAA 4 cut(s) 35, 208, 244, 418
AluBI AGCT 2 cut(s) 100, 239
AluI AGCT 2 cut(s) 100, 239
Alw26I GTCTC 2 cut(s) 51, 76
AlwI GGATC 2 cut(s) 151, 160
AlwNI CAGNNNCTG 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 501
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 4 cut(s) 77, 236, 313, 490
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp700I GAANNNNTTC 1 cut(s) 358
AvaI CYCGRG 1 cut(s) 377
BbvI GCAGC 4 cut(s) 89, 248, 300, 477
BccI CCATC 1 cut(s) 254
BcoDI GTCTC 2 cut(s) 51, 76
BfmI CTRYAG 1 cut(s) 75
BisI GCNGC 5 cut(s) 78, 237, 314, 422, 491
BlsI GCNGC 5 cut(s) 79, 238, 315, 423, 492
BmcAI AGTACT 1 cut(s) 476
BmeT110I CYCGRG 1 cut(s) 377
BmsI GCATC 1 cut(s) 89
Bpu10I CCTNAGC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 76
BsaWI WCCGGW 1 cut(s) 501
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 339
BseAI TCCGGA 1 cut(s) 501
BseGI GGATG 1 cut(s) 498
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 1 cut(s) 375
BseRI GAGGAG 1 cut(s) 233
BseXI GCAGC 4 cut(s) 89, 248, 300, 477
BsgI GTGCAG 1 cut(s) 108
Bsh1285I CGRYCG 1 cut(s) 337
BshFI GGCC 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 337
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 2 cut(s) 150, 502
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 2 cut(s) 51, 76
BsmI GAATGC 1 cut(s) 445
BsnI GGCC 1 cut(s) 108
Bso31I GGTCTC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 377
Bsp13I TCCGGA 1 cut(s) 501
Bsp143I GATC 4 cut(s) 143, 152, 169, 457
BspACI CCGC 3 cut(s) 190, 351, 421
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 1 cut(s) 376
BspEI TCCGGA 1 cut(s) 501
BspMAI CTGCAG 1 cut(s) 79
BspPI GGATC 2 cut(s) 151, 160
BspTNI GGTCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 339
BssMI GATC 4 cut(s) 143, 152, 169, 457
Bst4CI ACNGT 1 cut(s) 335
BstAPI GCANNNNNTGC 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 3 cut(s) 48, 117, 384
BstENI CCTNNNNNAGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 498
BstKTI GATC 4 cut(s) 146, 155, 172, 460
BstMAI GTCTC 2 cut(s) 51, 76
BstMBI GATC 4 cut(s) 143, 152, 169, 457
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 2 cut(s) 86, 122
BstNSI RCATGY 1 cut(s) 287
BstSFI CTRYAG 1 cut(s) 75
BstV1I GCAGC 4 cut(s) 89, 248, 300, 477
BstX2I RGATCY 2 cut(s) 143, 152
BstYI RGATCY 2 cut(s) 143, 152
BsuRI GGCC 1 cut(s) 108
BtsCI GGATG 1 cut(s) 498
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 127
CaiI CAGNNNCTG 1 cut(s) 83
CseI GACGC 1 cut(s) 314
Csp6I GTAC 3 cut(s) 250, 467, 475
CviAII CATG 3 cut(s) 284, 322, 434
CviQI GTAC 3 cut(s) 250, 467, 475
DdeI CTNAG 3 cut(s) 48, 117, 384
DpnI GATC 4 cut(s) 145, 154, 171, 459
DpnII GATC 4 cut(s) 143, 152, 169, 457
EaeI YGGCCR 1 cut(s) 106
Eco31I GGTCTC 1 cut(s) 76
Eco57I CTGAAG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 51
FaeI CATG 3 cut(s) 287, 325, 437
FaiI YATR 9 cut(s) 21, 40, 135, 285, 323, 431, 435, 464, 480
FatI CATG 3 cut(s) 283, 321, 433
Fnu4HI GCNGC 5 cut(s) 78, 237, 314, 422, 491
FokI GGATG 1 cut(s) 485
Fsp4HI GCNGC 5 cut(s) 78, 237, 314, 422, 491
GluI GCNGC 5 cut(s) 78, 237, 314, 422, 491
HaeIII GGCC 1 cut(s) 108
HapII CCGG 2 cut(s) 150, 502
HgaI GACGC 1 cut(s) 314
Hin1II CATG 3 cut(s) 287, 325, 437
HinfI GANTC 1 cut(s) 27
HpaII CCGG 2 cut(s) 150, 502
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 4 cut(s) 257, 377, 406, 502
HpyAV CCTTC 2 cut(s) 383, 499
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4V TGCA 3 cut(s) 77, 89, 413
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 122
HpyF3I CTNAG 3 cut(s) 48, 117, 384
Hsp92II CATG 3 cut(s) 287, 325, 437
Kpn2I TCCGGA 1 cut(s) 501
Kzo9I GATC 4 cut(s) 143, 152, 169, 457
LpnPI CCDG 7 cut(s) 9, 69, 111, 163, 270, 396, 419
Lsp1109I GCAGC 4 cut(s) 89, 248, 300, 477
LweI GCATC 1 cut(s) 89
MalI GATC 4 cut(s) 145, 154, 171, 459
MboI GATC 4 cut(s) 143, 152, 169, 457
MboII GAAGA 1 cut(s) 190
MflI RGATCY 2 cut(s) 143, 152
MluCI AATT 5 cut(s) 110, 208, 264, 296, 451
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 3 cut(s) 54, 193, 211
MroI TCCGGA 1 cut(s) 501
MroXI GAANNNNTTC 1 cut(s) 358
MslI CAYNNNNRTG 2 cut(s) 288, 344
MspI CCGG 2 cut(s) 150, 502
Mva1269I GAATGC 1 cut(s) 445
MwoI GCNNNNNNNGC 2 cut(s) 86, 122
NdeII GATC 4 cut(s) 143, 152, 169, 457
NlaIII CATG 3 cut(s) 287, 325, 437
NmeAIII GCCGAG 1 cut(s) 84
NspI RCATGY 1 cut(s) 287
PctI GAATGC 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 358
PfeI GAWTC 1 cut(s) 27
PkrI GCNGC 5 cut(s) 79, 238, 315, 423, 492
PstI CTGCAG 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 2 cut(s) 143, 152
RsaI GTAC 3 cut(s) 251, 468, 476
RsaNI GTAC 3 cut(s) 250, 467, 475
RseI CAYNNNNRTG 2 cut(s) 288, 344
SatI GCNGC 5 cut(s) 78, 237, 314, 422, 491
Sau3AI GATC 4 cut(s) 143, 152, 169, 457
ScaI AGTACT 1 cut(s) 476
SetI ASST 7 cut(s) 66, 102, 168, 241, 251, 375, 510
SfaNI GCATC 1 cut(s) 89
SfcI CTRYAG 1 cut(s) 75
SmiMI CAYNNNNRTG 2 cut(s) 288, 344
Sse9I AATT 5 cut(s) 110, 208, 264, 296, 451
SsiI CCGC 3 cut(s) 190, 351, 421
TaaI ACNGT 1 cut(s) 335
TasI AATT 5 cut(s) 110, 208, 264, 296, 451
TatI WGTACW 1 cut(s) 474
TauI GCSGC 1 cut(s) 424
TfiI GAWTC 1 cut(s) 27
TscAI CASTG 1 cut(s) 315
TseI GCWGC 4 cut(s) 77, 236, 313, 490
TspDTI ATGAA 1 cut(s) 338
TspRI CASTG 1 cut(s) 315
XagI CCTNNNNNAGG 1 cut(s) 51
XceI RCATGY 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 358
ZrmI AGTACT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.