2-alkenal reductase (NADP( )-dependent)-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 213, 609 |
| AarI | CACCTGC | 1 cut(s) | 28 |
| Acc36I | ACCTGC | 1 cut(s) | 28 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 224 |
| AccII | CGCG | 1 cut(s) | 140 |
| AccIII | TCCGGA | 1 cut(s) | 262 |
| AciI | CCGC | 1 cut(s) | 140 |
| AclWI | GGATC | 3 cut(s) | 181, 194, 343 |
| AcoI | YGGCCR | 1 cut(s) | 730 |
| AcsI | RAATTY | 1 cut(s) | 819 |
| AfaI | GTAC | 4 cut(s) | 89, 106, 366, 647 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 163, 224, 449, 559 |
| AflIII | ACRYGT | 1 cut(s) | 84 |
| AgsI | TTSAA | 5 cut(s) | 206, 346, 604, 640, 935 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 146, 178 |
| AluBI | AGCT | 5 cut(s) | 115, 479, 496, 583, 1019 |
| AluI | AGCT | 5 cut(s) | 115, 479, 496, 583, 1019 |
| Alw21I | GWGCWC | 1 cut(s) | 440 |
| Alw26I | GTCTC | 3 cut(s) | 323, 715, 805 |
| AlwI | GGATC | 3 cut(s) | 181, 194, 343 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 479 |
| Ama87I | CYCGRG | 2 cut(s) | 116, 941 |
| Aor13HI | TCCGGA | 1 cut(s) | 262 |
| AoxI | GGCC | 2 cut(s) | 730, 937 |
| ApeKI | GCWGC | 4 cut(s) | 473, 476, 632, 956 |
| ApoI | RAATTY | 1 cut(s) | 819 |
| AspS9I | GGNCC | 1 cut(s) | 950 |
| AsuC2I | CCSGG | 1 cut(s) | 402 |
| AsuHPI | GGTGA | 3 cut(s) | 23, 52, 290 |
| AvaI | CYCGRG | 2 cut(s) | 116, 941 |
| AvaII | GGWCC | 1 cut(s) | 950 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 88, 121, 348, 381 |
| BalI | TGGCCA | 1 cut(s) | 732 |
| BamHI | GGATCC | 1 cut(s) | 186 |
| BanII | GRGCYC | 2 cut(s) | 134, 972 |
| Bbv12I | GWGCWC | 1 cut(s) | 440 |
| BbvI | GCAGC | 4 cut(s) | 485, 488, 619, 943 |
| BccI | CCATC | 3 cut(s) | 297, 514, 864 |
| BceAI | ACGGC | 1 cut(s) | 725 |
| BcnI | CCSGG | 1 cut(s) | 402 |
| BcoDI | GTCTC | 3 cut(s) | 323, 715, 805 |
| BfaI | CTAG | 1 cut(s) | 584 |
| BfmI | CTRYAG | 1 cut(s) | 471 |
| BfuAI | ACCTGC | 1 cut(s) | 28 |
| BisI | GCNGC | 4 cut(s) | 474, 477, 633, 957 |
| BlsI | GCNGC | 4 cut(s) | 475, 478, 634, 958 |
| Bme1390I | CCNGG | 1 cut(s) | 402 |
| Bme18I | GGWCC | 1 cut(s) | 950 |
| BmeT110I | CYCGRG | 2 cut(s) | 116, 941 |
| BmgT120I | GGNCC | 1 cut(s) | 950 |
| BmiI | GGNNCC | 2 cut(s) | 123, 188 |
| BmrFI | CCNGG | 1 cut(s) | 402 |
| BmsI | GCATC | 3 cut(s) | 268, 619, 691 |
| BpmI | CTGGAG | 1 cut(s) | 999 |
| BpuMI | CCSGG | 1 cut(s) | 402 |
| BsaAI | YACGTR | 1 cut(s) | 91 |
| BsaI | GGTCTC | 1 cut(s) | 715 |
| BsaWI | WCCGGW | 2 cut(s) | 262, 299 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 444, 474 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 163, 224, 449, 559 |
| Bse1I | ACTGG | 2 cut(s) | 67, 388 |
| BseAI | TCCGGA | 1 cut(s) | 262 |
| BseGI | GGATG | 3 cut(s) | 259, 308, 706 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 163, 224, 449, 559 |
| BseMII | CTCAG | 1 cut(s) | 147 |
| BseNI | ACTGG | 2 cut(s) | 67, 388 |
| BseRI | GAGGAG | 1 cut(s) | 465 |
| BseXI | GCAGC | 4 cut(s) | 485, 488, 619, 943 |
| Bsh1236I | CGCG | 1 cut(s) | 140 |
| BshFI | GGCC | 2 cut(s) | 732, 939 |
| BsiHKAI | GWGCWC | 1 cut(s) | 440 |
| BsiHKCI | CYCGRG | 2 cut(s) | 116, 941 |
| BsiSI | CCGG | 4 cut(s) | 263, 300, 401, 953 |
| BslFI | GGGAC | 1 cut(s) | 65 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 163, 224, 449, 559 |
| BsmAI | GTCTC | 3 cut(s) | 323, 715, 805 |
| BsmBI | CGTCTC | 1 cut(s) | 805 |
| BsmFI | GGGAC | 1 cut(s) | 65 |
| BsnI | GGCC | 2 cut(s) | 732, 939 |
| Bso31I | GGTCTC | 1 cut(s) | 715 |
| BsoBI | CYCGRG | 2 cut(s) | 116, 941 |
| Bsp1286I | GDGCHC | 3 cut(s) | 134, 440, 972 |
| Bsp13I | TCCGGA | 1 cut(s) | 262 |
| Bsp143I | GATC | 4 cut(s) | 186, 348, 565, 753 |
| BspACI | CCGC | 1 cut(s) | 140 |
| BspANI | GGCC | 2 cut(s) | 732, 939 |
| BspCNI | CTCAG | 1 cut(s) | 146 |
| BspEI | TCCGGA | 1 cut(s) | 262 |
| BspFNI | CGCG | 1 cut(s) | 140 |
| BspLI | GGNNCC | 2 cut(s) | 123, 188 |
| BspMAI | CTGCAG | 1 cut(s) | 475 |
| BspMI | ACCTGC | 1 cut(s) | 28 |
| BspPI | GGATC | 3 cut(s) | 181, 194, 343 |
| BspQI | GCTCTTC | 1 cut(s) | 609 |
| BspTNI | GGTCTC | 1 cut(s) | 715 |
| BsrI | ACTGG | 2 cut(s) | 67, 388 |
| BssMI | GATC | 4 cut(s) | 186, 348, 565, 753 |
| Bst4CI | ACNGT | 1 cut(s) | 101 |
| Bst6I | CTCTTC | 3 cut(s) | 303, 347, 609 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 482 |
| BstBAI | YACGTR | 1 cut(s) | 91 |
| BstC8I | GCNNGC | 1 cut(s) | 440 |
| BstDEI | CTNAG | 1 cut(s) | 133 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 447 |
| BstF5I | GGATG | 3 cut(s) | 259, 308, 706 |
| BstFNI | CGCG | 1 cut(s) | 140 |
| BstKTI | GATC | 4 cut(s) | 189, 351, 568, 756 |
| BstMAI | GTCTC | 3 cut(s) | 323, 715, 805 |
| BstMBI | GATC | 4 cut(s) | 186, 348, 565, 753 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 482, 502 |
| BstNSI | RCATGY | 1 cut(s) | 88 |
| BstSCI | CCNGG | 1 cut(s) | 400 |
| BstSFI | CTRYAG | 1 cut(s) | 471 |
| BstUI | CGCG | 1 cut(s) | 140 |
| BstV1I | GCAGC | 4 cut(s) | 485, 488, 619, 943 |
| BstX2I | RGATCY | 2 cut(s) | 186, 348 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 874 |
| BstYI | RGATCY | 2 cut(s) | 186, 348 |
| BsuRI | GGCC | 2 cut(s) | 732, 939 |
| BtsCI | GGATG | 3 cut(s) | 259, 308, 706 |
| BtsIMutI | CAGTG | 1 cut(s) | 357 |
| BveI | ACCTGC | 1 cut(s) | 28 |
| Cac8I | GCNNGC | 1 cut(s) | 440 |
| CaiI | CAGNNNCTG | 1 cut(s) | 479 |
| Cfr13I | GGNCC | 1 cut(s) | 950 |
| Csp6I | GTAC | 4 cut(s) | 88, 105, 365, 646 |
| CviAII | CATG | 6 cut(s) | 85, 428, 620, 718, 728, 880 |
| CviQI | GTAC | 4 cut(s) | 88, 105, 365, 646 |
| DdeI | CTNAG | 1 cut(s) | 133 |
| DpnI | GATC | 4 cut(s) | 188, 350, 567, 755 |
| DpnII | GATC | 4 cut(s) | 186, 348, 565, 753 |
| EaeI | YGGCCR | 1 cut(s) | 730 |
| Eam1104I | CTCTTC | 3 cut(s) | 303, 347, 609 |
| EarI | CTCTTC | 3 cut(s) | 303, 347, 609 |
| Eco147I | AGGCCT | 1 cut(s) | 939 |
| Eco24I | GRGCYC | 2 cut(s) | 134, 972 |
| Eco31I | GGTCTC | 1 cut(s) | 715 |
| Eco47I | GGWCC | 1 cut(s) | 950 |
| Eco88I | CYCGRG | 2 cut(s) | 116, 941 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 447 |
| EcoRI | GAATTC | 1 cut(s) | 819 |
| EcoT38I | GRGCYC | 2 cut(s) | 134, 972 |
| Esp3I | CGTCTC | 1 cut(s) | 805 |
| FaeI | CATG | 6 cut(s) | 88, 431, 623, 721, 731, 883 |
| FaqI | GGGAC | 1 cut(s) | 65 |
| FatI | CATG | 6 cut(s) | 84, 427, 619, 717, 727, 879 |
| Fnu4HI | GCNGC | 4 cut(s) | 474, 477, 633, 957 |
| FokI | GGATG | 3 cut(s) | 246, 315, 713 |
| FriOI | GRGCYC | 2 cut(s) | 134, 972 |
| Fsp4HI | GCNGC | 4 cut(s) | 474, 477, 633, 957 |
| FspBI | CTAG | 1 cut(s) | 584 |
| GluI | GCNGC | 4 cut(s) | 474, 477, 633, 957 |
| GsuI | CTGGAG | 1 cut(s) | 999 |
| HaeIII | GGCC | 2 cut(s) | 732, 939 |
| HapII | CCGG | 4 cut(s) | 263, 300, 401, 953 |
| Hin1II | CATG | 6 cut(s) | 88, 431, 623, 721, 731, 883 |
| HinfI | GANTC | 3 cut(s) | 77, 251, 455 |
| HpaII | CCGG | 4 cut(s) | 263, 300, 401, 953 |
| HphI | GGTGA | 3 cut(s) | 23, 52, 290 |
| Hpy188I | TCNGA | 5 cut(s) | 82, 191, 548, 899, 992 |
| Hpy188III | TCNNGA | 6 cut(s) | 151, 263, 346, 676, 920, 943 |
| HpyAV | CCTTC | 6 cut(s) | 200, 269, 553, 719, 824, 929 |
| HpyCH4III | ACNGT | 1 cut(s) | 101 |
| HpyCH4IV | ACGT | 2 cut(s) | 90, 363 |
| HpyCH4V | TGCA | 3 cut(s) | 473, 512, 704 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 482, 502 |
| HpyF3I | CTNAG | 1 cut(s) | 133 |
| HpySE526I | ACGT | 2 cut(s) | 90, 363 |
| Hsp92II | CATG | 6 cut(s) | 88, 431, 623, 721, 731, 883 |
| Kpn2I | TCCGGA | 1 cut(s) | 262 |
| Kzo9I | GATC | 4 cut(s) | 186, 348, 565, 753 |
| LguI | GCTCTTC | 1 cut(s) | 609 |
| LmnI | GCTCC | 1 cut(s) | 1018 |
| Lsp1109I | GCAGC | 4 cut(s) | 485, 488, 619, 943 |
| LweI | GCATC | 3 cut(s) | 268, 619, 691 |
| MaeI | CTAG | 1 cut(s) | 584 |
| MaeII | ACGT | 2 cut(s) | 90, 363 |
| MaeIII | GTNAC | 3 cut(s) | 91, 170, 278 |
| MalI | GATC | 4 cut(s) | 188, 350, 567, 755 |
| MboI | GATC | 4 cut(s) | 186, 348, 565, 753 |
| MboII | GAAGA | 4 cut(s) | 320, 334, 626, 935 |
| MflI | RGATCY | 2 cut(s) | 186, 348 |
| MhlI | GDGCHC | 3 cut(s) | 134, 440, 972 |
| MlsI | TGGCCA | 1 cut(s) | 732 |
| MluCI | AATT | 4 cut(s) | 201, 506, 604, 819 |
| MluNI | TGGCCA | 1 cut(s) | 732 |
| MlyI | GAGTC | 1 cut(s) | 86 |
| MmeI | TCCRAC | 4 cut(s) | 60, 517, 526, 970 |
| MnlI | CCTC | 8 cut(s) | 7, 21, 112, 177, 443, 589, 735, 950 |
| Mox20I | TGGCCA | 1 cut(s) | 732 |
| MroI | TCCGGA | 1 cut(s) | 262 |
| MscI | TGGCCA | 1 cut(s) | 732 |
| MseI | TTAA | 2 cut(s) | 111, 270 |
| MslI | CAYNNNNRTG | 1 cut(s) | 89 |
| Msp20I | TGGCCA | 1 cut(s) | 732 |
| MspA1I | CMGCKG | 2 cut(s) | 479, 1019 |
| MspI | CCGG | 4 cut(s) | 263, 300, 401, 953 |
| MspR9I | CCNGG | 1 cut(s) | 402 |
| MvnI | CGCG | 1 cut(s) | 140 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 482, 502 |
| NciI | CCSGG | 1 cut(s) | 402 |
| NdeII | GATC | 4 cut(s) | 186, 348, 565, 753 |
| NlaIII | CATG | 6 cut(s) | 88, 431, 623, 721, 731, 883 |
| NlaIV | GGNNCC | 2 cut(s) | 123, 188 |
| NmuCI | GTSAC | 3 cut(s) | 91, 170, 278 |
| NspI | RCATGY | 1 cut(s) | 88 |
| PaeR7I | CTCGAG | 2 cut(s) | 116, 941 |
| PaqCI | CACCTGC | 1 cut(s) | 28 |
| PceI | AGGCCT | 1 cut(s) | 939 |
| PciI | ACATGT | 1 cut(s) | 84 |
| PciSI | GCTCTTC | 1 cut(s) | 609 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 843 |
| PfeI | GAWTC | 2 cut(s) | 251, 455 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 224 |
| PkrI | GCNGC | 4 cut(s) | 475, 478, 634, 958 |
| PleI | GAGTC | 1 cut(s) | 85 |
| PpsI | GAGTC | 1 cut(s) | 85 |
| Ppu21I | YACGTR | 1 cut(s) | 91 |
| PscI | ACATGT | 1 cut(s) | 84 |
| PsiI | TTATAA | 2 cut(s) | 213, 609 |
| PspN4I | GGNNCC | 2 cut(s) | 123, 188 |
| PspPI | GGNCC | 1 cut(s) | 950 |
| PspXI | VCTCGAGB | 1 cut(s) | 116 |
| PstI | CTGCAG | 1 cut(s) | 475 |
| PstNI | CAGNNNCTG | 1 cut(s) | 479 |
| PsuI | RGATCY | 2 cut(s) | 186, 348 |
| PvuII | CAGCTG | 2 cut(s) | 479, 1019 |
| RsaI | GTAC | 4 cut(s) | 89, 106, 366, 647 |
| RsaNI | GTAC | 4 cut(s) | 88, 105, 365, 646 |
| RseI | CAYNNNNRTG | 1 cut(s) | 89 |
| SapI | GCTCTTC | 1 cut(s) | 609 |
| SaqAI | TTAA | 2 cut(s) | 111, 270 |
| SatI | GCNGC | 4 cut(s) | 474, 477, 633, 957 |
| Sau3AI | GATC | 4 cut(s) | 186, 348, 565, 753 |
| Sau96I | GGNCC | 1 cut(s) | 950 |
| SchI | GAGTC | 1 cut(s) | 86 |
| ScrFI | CCNGG | 1 cut(s) | 402 |
| SduI | GDGCHC | 3 cut(s) | 134, 440, 972 |
| SfaNI | GCATC | 3 cut(s) | 268, 619, 691 |
| SfcI | CTRYAG | 1 cut(s) | 471 |
| Sfr274I | CTCGAG | 2 cut(s) | 116, 941 |
| SinI | GGWCC | 1 cut(s) | 950 |
| SlaI | CTCGAG | 2 cut(s) | 116, 941 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 89 |
| SmlI | CTYRAG | 2 cut(s) | 116, 941 |
| SmoI | CTYRAG | 2 cut(s) | 116, 941 |
| Sse9I | AATT | 4 cut(s) | 201, 506, 604, 819 |
| SseBI | AGGCCT | 1 cut(s) | 939 |
| SsiI | CCGC | 1 cut(s) | 140 |
| SspMI | CTAG | 1 cut(s) | 584 |
| StuI | AGGCCT | 1 cut(s) | 939 |
| StyD4I | CCNGG | 1 cut(s) | 400 |
| TaaI | ACNGT | 1 cut(s) | 101 |
| TaiI | ACGT | 2 cut(s) | 93, 366 |
| TaqI | TCGA | 6 cut(s) | 117, 564, 675, 817, 846, 942 |
| TasI | AATT | 4 cut(s) | 201, 506, 604, 819 |
| TfiI | GAWTC | 2 cut(s) | 251, 455 |
| Tru1I | TTAA | 2 cut(s) | 111, 270 |
| Tru9I | TTAA | 2 cut(s) | 111, 270 |
| TscAI | CASTG | 1 cut(s) | 364 |
| TseFI | GTSAC | 3 cut(s) | 91, 170, 278 |
| TseI | GCWGC | 4 cut(s) | 473, 476, 632, 956 |
| Tsp45I | GTSAC | 3 cut(s) | 91, 170, 278 |
| TspDTI | ATGAA | 1 cut(s) | 444 |
| TspRI | CASTG | 1 cut(s) | 364 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 224 |
| VpaK11BI | GGWCC | 1 cut(s) | 950 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 447 |
| XapI | RAATTY | 1 cut(s) | 819 |
| XceI | RCATGY | 1 cut(s) | 88 |
| XhoI | CTCGAG | 2 cut(s) | 116, 941 |
| XspI | CTAG | 1 cut(s) | 584 |