pycom16g25160

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000431
Physical Location & Seq
Forward (+)
26969780 .. 26970757
978 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g25160.1

Sequence Viewer

Length: 420 bp
ATGCACGTGACAGTTAGTACCTTGAAGCTCAAGGTTCCACAGGACTCCAAGGCAGTGGTGGTCAAGAACCTTTACTTGTCGTGCGACCCTTATTTGAGGCTTCGAATGGATGGAACTCAACCTCCTAATAGTGTCTTCACTTCACTCACTCCTGGCTCTCCATTAAATGGGTTTGGTGTGGCTAAAATTTTGGAGTCGGGGCACCCGGAGTTCAAGGAAGGTGACTTGGTTTGGGGGACAACAGGATGGGAAGAGTACAGCATCTTCACAGAGCCAGAACAGCTCTTTAAAATCCACCCCACTGATGTTCCCCTTGCCTACTGTACTGGACTTCTTGGTCTGTGCCAGTTCTTCATCAAAACTATGAGTTATACATGTTCATACTTTGTACAATCACTATTTAATCCTTTATTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

140

Amino Acids

15.58

Weight (kDa)

5.85

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 13 - 97 1.8e-19 N-terminal domain of oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26320 AT1G26320 AT3G03080 AT3G59845 AT3G59845 AT5G16960 AT5G16960 AT5G16970 AT5G16980 AT5G16980 AT5G16980 AT5G16990 AT5G17000 AT5G17000 AT5G37940 AT5G37960 AT5G37980 AT5G38000 AT5G38000 AT5G38000
fragaria_vesca FvH4_1g00340 FvH4_1g18790 FvH4_1g23120 FvH4_1g23120
malus_domestica MD00G1045700.v1.1 MD01G1046700.v1.1 MD02G1163900.v1.1 MD15G1145600.v1.1 MD16G1282000.v1.1
prunus_persica Prupe.1G543700_v2.0.a1 Prupe.4G262000_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.6G211500_v2.0.a1 Prupe.7G269100_v2.0.a1
pyrus_communis pycom02g00040 pycom02g13040 pycom11g16640 pycom12561g00320 pycom15g13080 pycom16g25160
rosa_chinensis RchiOBHm_Chr2g0084641 RchiOBHm_Chr2g0084711 RchiOBHm_Chr2g0085361 RchiOBHm_Chr2g0085371 RchiOBHm_Chr2g0109651 RchiOBHm_Chr2g0117841 RchiOBHm_Chr6g0275751
rosa_laevigata RLG00000013436 RLG00000015629 RLG00000015633 RLG00000015680 RLG00000015681 RLG00000017753 RLG00000018376
rosa_multiflora Rmu_sc0000332.1_g000060 Rmu_sc0002352.1_g000008 Rmu_sc0002352.1_g000012 Rmu_sc0010860.1_g000005 Rmu_sc0012119.1_g000008
rosa_roxburghii Rroxscaffold_2G00126190 Rroxscaffold_2G00134060 Rroxscaffold_2G00155420 Rroxscaffold_2G00155430 Rroxscaffold_2G00155880 Rroxscaffold_2G00155890
rosa_rugosa Rorug01G0455500 Rorug01G0455600 Rorug01G0459700 Rorug01G0459800 Rorug01G0459800 Rorug01G0459900 Rorug02G0161600 Rorug02G0214400
rosa_samantha Rh2BG008800 Rh2BG282100 Rh2CG003900 Rh2CG004600 Rh2CG009500 Rh2CG009600 Rh2CG216100 Rh2CG216200 Rh2CG269100 Rh2CG269200 Rh2DG003700 Rh2DG004100 Rh2DG009900 Rh2DG219300 Rh2DG278400
rosa_wichuraiana Rw2G000300 Rw2G000330 Rw2G000770 Rw2G000780 Rw2G021390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 336
AccB1I GGYRCC 1 cut(s) 201
AccB7I CCANNNNNTGG 1 cut(s) 167
AcsI RAATTY 1 cut(s) 186
AcvI CACGTG 1 cut(s) 7
AfaI GTAC 4 cut(s) 19, 257, 325, 390
AfiI CCNNNNNNNGG 1 cut(s) 167
AflIII ACRYGT 1 cut(s) 374
AgsI TTSAA 2 cut(s) 25, 214
AjnI CCWGG 1 cut(s) 151
AloI GAACNNNNNNTCC 2 cut(s) 106, 138
AluBI AGCT 2 cut(s) 28, 283
AluI AGCT 2 cut(s) 28, 283
ApoI RAATTY 1 cut(s) 186
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 1 cut(s) 233
AsuII TTCGAA 1 cut(s) 103
BaeGI GKGCMC 1 cut(s) 204
BanI GGYRCC 1 cut(s) 201
BarI GAAGNNNNNNTAC 2 cut(s) 248, 280
BbrPI CACGTG 1 cut(s) 7
BbsI GAAGAC 1 cut(s) 127
BccI CCATC 2 cut(s) 104, 240
BciT130I CCWGG 1 cut(s) 153
BcnI CCSGG 1 cut(s) 206
Bme1390I CCNGG 2 cut(s) 153, 206
BmiI GGNNCC 2 cut(s) 36, 203
BmrFI CCNGG 2 cut(s) 153, 206
BmsI GCATC 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 127
Bpu14I TTCGAA 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 206
BsaAI YACGTR 1 cut(s) 7
BsaJI CCNNGG 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 331, 346
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 2 cut(s) 115, 251
BseLI CCNNNNNNNGG 1 cut(s) 167
BseNI ACTGG 2 cut(s) 331, 346
BseSI GKGCMC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 201
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 250
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 388
BspLI GGNNCC 2 cut(s) 36, 203
BspT104I TTCGAA 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 201
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 2 cut(s) 331, 346
BssECI CCNNGG 1 cut(s) 48
BssT1I CCWWGG 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 2 cut(s) 13, 323
Bst6I CTCTTC 1 cut(s) 246
BstAUI TGTACA 1 cut(s) 388
BstBAI YACGTR 1 cut(s) 7
BstBI TTCGAA 1 cut(s) 103
BstF5I GGATG 2 cut(s) 115, 251
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 1 cut(s) 378
BstSCI CCNGG 2 cut(s) 151, 204
BstSLI GKGCMC 1 cut(s) 204
BstV2I GAAGAC 1 cut(s) 127
BstXI CCANNNNNNTGG 1 cut(s) 55
BtsCI GGATG 2 cut(s) 115, 251
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 60, 300
Csp6I GTAC 4 cut(s) 18, 256, 324, 389
CviAII CATG 1 cut(s) 375
CviJI RGCY 6 cut(s) 28, 100, 156, 182, 274, 283
CviKI_1 RGCY 6 cut(s) 28, 100, 156, 182, 274, 283
CviQI GTAC 4 cut(s) 18, 256, 324, 389
DraI TTTAAA 1 cut(s) 289
DrdI GACNNNNNNGTC 1 cut(s) 336
DseDI GACNNNNNNGTC 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 48
Eco72I CACGTG 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 151
EcoT14I CCWWGG 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 1 cut(s) 378
FaiI YATR 4 cut(s) 365, 372, 376, 382
FaqI GGGAC 1 cut(s) 250
FatI CATG 1 cut(s) 374
FokI GGATG 2 cut(s) 122, 258
HapII CCGG 1 cut(s) 206
Hin1II CATG 1 cut(s) 378
HinfI GANTC 2 cut(s) 44, 194
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 233
Hpy188III TCNNGA 1 cut(s) 64
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 2 cut(s) 13, 323
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 1 cut(s) 378
LpnPI CCDG 8 cut(s) 26, 138, 165, 219, 228, 288, 312, 359
LweI GCATC 1 cut(s) 270
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 2 cut(s) 7, 221
MboII GAAGA 4 cut(s) 127, 256, 263, 343
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 1 cut(s) 186
MlyI GAGTC 2 cut(s) 38, 203
MnlI CCTC 2 cut(s) 90, 132
MseI TTAA 3 cut(s) 164, 288, 402
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 153, 206
MvaI CCWGG 1 cut(s) 153
MwoI GCNNNNNNNGC 1 cut(s) 280
NciI CCSGG 1 cut(s) 206
NlaIII CATG 1 cut(s) 378
NlaIV GGNNCC 2 cut(s) 36, 203
NmuCI GTSAC 2 cut(s) 7, 221
NspI RCATGY 1 cut(s) 378
NspV TTCGAA 1 cut(s) 103
PciI ACATGT 1 cut(s) 374
PflMI CCANNNNNTGG 1 cut(s) 167
PleI GAGTC 2 cut(s) 38, 202
PmaCI CACGTG 1 cut(s) 7
PmlI CACGTG 1 cut(s) 7
PpsI GAGTC 2 cut(s) 38, 202
Ppu21I YACGTR 1 cut(s) 7
PscI ACATGT 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 151
PspCI CACGTG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 2 cut(s) 36, 203
RsaI GTAC 4 cut(s) 19, 257, 325, 390
RsaNI GTAC 4 cut(s) 18, 256, 324, 389
SaqAI TTAA 3 cut(s) 164, 288, 402
SchI GAGTC 2 cut(s) 38, 203
ScrFI CCNGG 2 cut(s) 153, 206
SduI GDGCHC 1 cut(s) 204
SetI ASST 8 cut(s) 9, 23, 30, 36, 72, 124, 223, 285
SfaNI GCATC 1 cut(s) 270
SfuI TTCGAA 1 cut(s) 103
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 1 cut(s) 186
StyD4I CCNGG 2 cut(s) 151, 204
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 2 cut(s) 13, 323
TaiI ACGT 1 cut(s) 9
TaqI TCGA 1 cut(s) 103
TasI AATT 1 cut(s) 186
TatI WGTACW 3 cut(s) 255, 323, 388
Tru1I TTAA 3 cut(s) 164, 288, 402
Tru9I TTAA 3 cut(s) 164, 288, 402
TscAI CASTG 2 cut(s) 60, 307
TseFI GTSAC 2 cut(s) 7, 221
Tsp45I GTSAC 2 cut(s) 7, 221
TspDTI ATGAA 2 cut(s) 343, 369
TspRI CASTG 2 cut(s) 60, 307
Van91I CCANNNNNTGG 1 cut(s) 167
XapI RAATTY 1 cut(s) 186
XceI RCATGY 1 cut(s) 378
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.