Rorug02G0214400

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000431
Physical Location & Seq
Forward (+)
20487733 .. 20488596
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0214400.1

Sequence Viewer

Length: 612 bp
ATGAGTGAAACAATTTTATCATCTGCAGAGGGAGTGCAGGCATGTGCATGGGCTGCTAGAGATTCCTCAGGAATTCTCTCTCCCTTCCACTTCACCAGGAGGACTACTGGAAGTAATGATATCAGCATGAAGATACTCTACTGTGGTATTTGCCACTCAGACCTTGACCTTGCAAAGGACGAAGTTGGAATGACCATCTATCGGTATCCCATCGTCCCAGGACATGAGATCATCGGAGAAGTGACCGAAGTCGGAAGCAACGTCGCGAAATTCAAAGTAGGAGACATAGCCGGAGTGGGGTGCATGGTGGTTTCATGTGGTTCGTGCAATAACCGCACACGAGACTTGGAAAGTTACTGCCCCAATATGATATGGACCTATAACAAACACTACCACGACGGATCCAGAACTTTTGGGGGATACTCCGACAAACTAGTAGTCGAGGAGCATTTCGCAGTTCGAATTCCAGATAACTTGTCATTGGAAGATGTTGCACCTTTGCTATGTGCTGGGATTACGGTGTACAGCCCTATGAAACACTTTGGACTTTTTGAGCATGGTAAGCACTTGGGTGTGGTAGGGCTTGGTGGCAGTAGCAGATTTAGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

203

Amino Acids

22.16

Weight (kDa)

6.12

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 38 - 157 8.6e-22 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26320 AT1G26320 AT3G03080 AT3G59845 AT3G59845 AT5G16960 AT5G16960 AT5G16970 AT5G16980 AT5G16980 AT5G16980 AT5G16990 AT5G17000 AT5G17000 AT5G37940 AT5G37960 AT5G37980 AT5G38000 AT5G38000 AT5G38000
fragaria_vesca FvH4_1g00340 FvH4_1g18790 FvH4_1g23120 FvH4_1g23120
malus_domestica MD00G1045700.v1.1 MD01G1046700.v1.1 MD02G1163900.v1.1 MD15G1145600.v1.1 MD16G1282000.v1.1
prunus_persica Prupe.1G543700_v2.0.a1 Prupe.4G262000_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.6G211500_v2.0.a1 Prupe.7G269100_v2.0.a1
pyrus_communis pycom02g00040 pycom02g13040 pycom11g16640 pycom12561g00320 pycom15g13080 pycom16g25160
rosa_chinensis RchiOBHm_Chr2g0084641 RchiOBHm_Chr2g0084711 RchiOBHm_Chr2g0085361 RchiOBHm_Chr2g0085371 RchiOBHm_Chr2g0109651 RchiOBHm_Chr2g0117841 RchiOBHm_Chr6g0275751
rosa_laevigata RLG00000013436 RLG00000015629 RLG00000015633 RLG00000015680 RLG00000015681 RLG00000017753 RLG00000018376
rosa_multiflora Rmu_sc0000332.1_g000060 Rmu_sc0002352.1_g000008 Rmu_sc0002352.1_g000012 Rmu_sc0010860.1_g000005 Rmu_sc0012119.1_g000008
rosa_roxburghii Rroxscaffold_2G00126190 Rroxscaffold_2G00134060 Rroxscaffold_2G00155420 Rroxscaffold_2G00155430 Rroxscaffold_2G00155880 Rroxscaffold_2G00155890
rosa_rugosa Rorug01G0455500 Rorug01G0455600 Rorug01G0459700 Rorug01G0459800 Rorug01G0459800 Rorug01G0459900 Rorug02G0161600 Rorug02G0214400
rosa_samantha Rh2BG008800 Rh2BG282100 Rh2CG003900 Rh2CG004600 Rh2CG009500 Rh2CG009600 Rh2CG216100 Rh2CG216200 Rh2CG269100 Rh2CG269200 Rh2DG003700 Rh2DG004100 Rh2DG009900 Rh2DG219300 Rh2DG278400
rosa_wichuraiana Rw2G000300 Rw2G000330 Rw2G000770 Rw2G000780 Rw2G021390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 266
AciI CCGC 1 cut(s) 334
AclWI GGATC 2 cut(s) 396, 409
AcsI RAATTY 4 cut(s) 72, 269, 462, 606
AfaI GTAC 1 cut(s) 524
AfiI CCNNNNNNNGG 3 cut(s) 175, 201, 297
AgsI TTSAA 1 cut(s) 274
AhlI ACTAGT 1 cut(s) 433
AjnI CCWGG 2 cut(s) 95, 217
AleI CACNNNNGTG 1 cut(s) 570
Alw26I GTCTC 2 cut(s) 276, 336
AlwI GGATC 2 cut(s) 396, 409
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 4 cut(s) 72, 269, 462, 606
ArsI GACNNNNNNTTYG 2 cut(s) 423, 455
AspS9I GGNCC 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 85
AsuII TTCGAA 1 cut(s) 460
AvaII GGWCC 1 cut(s) 375
AxyI CCTNAGG 1 cut(s) 67
BamHI GGATCC 1 cut(s) 401
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
BauI CACGAG 1 cut(s) 339
BbvI GCAGC 1 cut(s) 40
BccI CCATC 2 cut(s) 203, 218
BciT130I CCWGG 2 cut(s) 97, 219
BciVI GTATCC 2 cut(s) 216, 413
BcoDI GTCTC 2 cut(s) 276, 336
BcuI ACTAGT 1 cut(s) 433
BfaI CTAG 2 cut(s) 57, 434
BfmI CTRYAG 1 cut(s) 24
BfuI GTATCC 2 cut(s) 216, 413
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 2 cut(s) 97, 219
Bme18I GGWCC 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 2 cut(s) 97, 219
BoxI GACNNNNGTC 1 cut(s) 248
Bpu14I TTCGAA 1 cut(s) 460
BsaJI CCNNGG 1 cut(s) 217
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 201, 297
Bse1I ACTGG 1 cut(s) 112
Bse21I CCTNAGG 1 cut(s) 67
BseBI CCWGG 2 cut(s) 97, 219
BseDI CCNNGG 1 cut(s) 217
BseLI CCNNNNNNNGG 3 cut(s) 175, 201, 297
BseMII CTCAG 2 cut(s) 81, 171
BseNI ACTGG 1 cut(s) 112
BseRI GAGGAG 1 cut(s) 458
BseXI GCAGC 1 cut(s) 40
BseYI CCCAGC 1 cut(s) 509
BsgI GTGCAG 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 3 cut(s) 175, 201, 297
BsmAI GTCTC 2 cut(s) 276, 336
BsmFI GGGAC 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 460
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 2 cut(s) 228, 401
Bsp68I TCGCGA 1 cut(s) 266
BspACI CCGC 1 cut(s) 334
BspCNI CTCAG 2 cut(s) 80, 170
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 403
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 2 cut(s) 396, 409
BspT104I TTCGAA 1 cut(s) 460
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 2 cut(s) 228, 401
BssSI CACGAG 1 cut(s) 339
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 2 cut(s) 97, 219
Bst4CI ACNGT 2 cut(s) 143, 520
BstAPI GCANNNNNTGC 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 522
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 2 cut(s) 67, 157
BstENI CCTNNNNNAGG 1 cut(s) 173
BstFNI CGCG 1 cut(s) 266
BstKTI GATC 2 cut(s) 231, 404
BstMAI GTCTC 2 cut(s) 276, 336
BstMBI GATC 2 cut(s) 228, 401
BstMWI GCNNNNNNNGC 3 cut(s) 53, 333, 562
BstNI CCWGG 2 cut(s) 97, 219
BstNSI RCATGY 1 cut(s) 45
BstPAI GACNNNNGTC 1 cut(s) 248
BstSCI CCNGG 2 cut(s) 95, 217
BstSFI CTRYAG 1 cut(s) 24
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
Bsu36I CCTNAGG 1 cut(s) 67
BsuI GTATCC 2 cut(s) 216, 413
BtuMI TCGCGA 1 cut(s) 266
Cac8I GCNNGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 375
Csp6I GTAC 1 cut(s) 523
CviAII CATG 7 cut(s) 42, 48, 127, 224, 304, 315, 557
CviJI RGCY 4 cut(s) 53, 290, 528, 583
CviKI_1 RGCY 4 cut(s) 53, 290, 528, 583
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 2 cut(s) 67, 157
DpnI GATC 2 cut(s) 230, 403
DpnII GATC 2 cut(s) 228, 401
Eco32I GATATC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 375
Eco81I CCTNAGG 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoRI GAATTC 2 cut(s) 72, 462
EcoRII CCWGG 2 cut(s) 95, 217
EcoRV GATATC 1 cut(s) 121
FaeI CATG 7 cut(s) 45, 51, 130, 227, 307, 318, 560
FaqI GGGAC 1 cut(s) 200
FatI CATG 7 cut(s) 41, 47, 126, 223, 303, 314, 556
Fnu4HI GCNGC 1 cut(s) 54
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 2 cut(s) 57, 434
GluI GCNGC 1 cut(s) 54
GsaI CCCAGC 1 cut(s) 513
HapII CCGG 1 cut(s) 291
Hin1II CATG 7 cut(s) 45, 51, 130, 227, 307, 318, 560
HinfI GANTC 1 cut(s) 62
HpaII CCGG 1 cut(s) 291
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 4 cut(s) 160, 236, 254, 427
Hpy188III TCNNGA 4 cut(s) 69, 265, 405, 467
Hpy8I GTNNAC 1 cut(s) 523
Hpy99I CGWCG 2 cut(s) 266, 401
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 143, 520
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 7 cut(s) 26, 37, 47, 173, 303, 327, 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 333, 562
HpyF3I CTNAG 2 cut(s) 67, 157
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 7 cut(s) 45, 51, 130, 227, 307, 318, 560
Kzo9I GATC 2 cut(s) 228, 401
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 1 cut(s) 40
MaeI CTAG 2 cut(s) 57, 434
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 241, 353
MalI GATC 2 cut(s) 230, 403
MboI GATC 2 cut(s) 228, 401
MboII GAAGA 2 cut(s) 142, 497
MflI RGATCY 1 cut(s) 401
MluCI AATT 5 cut(s) 12, 72, 269, 462, 606
MmeI TCCRAC 3 cut(s) 166, 232, 450
MnlI CCTC 4 cut(s) 22, 76, 93, 436
MslI CAYNNNNRTG 2 cut(s) 46, 570
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 2 cut(s) 97, 219
MvaI CCWGG 2 cut(s) 97, 219
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 3 cut(s) 53, 333, 562
NdeII GATC 2 cut(s) 228, 401
NlaIII CATG 7 cut(s) 45, 51, 130, 227, 307, 318, 560
NlaIV GGNNCC 1 cut(s) 403
NmuCI GTSAC 1 cut(s) 241
NruI TCGCGA 1 cut(s) 266
NspI RCATGY 1 cut(s) 45
NspV TTCGAA 1 cut(s) 460
OliI CACNNNNGTG 1 cut(s) 570
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 1 cut(s) 55
PshAI GACNNNNGTC 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 95, 217
PspFI CCCAGC 1 cut(s) 509
PspGI CCWGG 2 cut(s) 95, 217
PspN4I GGNNCC 1 cut(s) 403
PspPI GGNCC 1 cut(s) 375
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 401
RruI TCGCGA 1 cut(s) 266
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
RseI CAYNNNNRTG 2 cut(s) 46, 570
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 2 cut(s) 228, 401
Sau96I GGNCC 1 cut(s) 375
ScrFI CCNGG 2 cut(s) 97, 219
SetI ASST 5 cut(s) 165, 171, 264, 380, 499
SfcI CTRYAG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 460
SinI GGWCC 1 cut(s) 375
SmiMI CAYNNNNRTG 2 cut(s) 46, 570
SpeI ACTAGT 1 cut(s) 433
Sse9I AATT 5 cut(s) 12, 72, 269, 462, 606
SsiI CCGC 1 cut(s) 334
SspMI CTAG 2 cut(s) 57, 434
StyD4I CCNGG 2 cut(s) 95, 217
TaaI ACNGT 2 cut(s) 143, 520
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 441, 460
TaqII GACCGA 1 cut(s) 260
TasI AATT 5 cut(s) 12, 72, 269, 462, 606
TatI WGTACW 1 cut(s) 522
TfiI GAWTC 1 cut(s) 62
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 3 cut(s) 143, 303, 548
TspGWI ACGGA 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 375
XagI CCTNNNNNAGG 1 cut(s) 173
XapI RAATTY 4 cut(s) 72, 269, 462, 606
XceI RCATGY 1 cut(s) 45
XspI CTAG 2 cut(s) 57, 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.