Rw2G000330

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000431
Physical Location & Seq
Forward (+)
321139 .. 328074
6936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G000330.1

Sequence Viewer

Length: 1062 bp
ATGGCAGGTGAAGTAGCAGTGAGGAATAAGCAGGTAATACCGAGGGACTATGTGTCTGGTTTTCCCAAAGAGTCAGACATGTATGTCACAACTGATAGCACGGTAAGTCTGAAGGTTCCAGCAGAGACGAGAAATGCTGTTCTACTCAAGAACCTGTACTTGTCCTGCGATCCCATGATGCAATTCCTCATTAGGAATAACACTCAGCAGAAAATGAGAAAATACATCTATTGCACTCCTGGCTCTCCAATTCGTGGGTATGGTGTGTCTCAAGTTGTGGACTCGAGTCATCCAGAGTATAAACAAGGTGATTTGGTGTGGGGGTTAACCACATGGGAAGAGTACAGCCTCATCACAGATATCGTCCCTGAAACCTCACTCGTTAAAATACACCACACTGATGTCCCTCTTTCTTACTATACTGGACTTCTTGGCATGGCTGGTAAGACTGCTTATGCTGGATTCTTTGAAGTATGTCCTCCTAAGAAAGGAGACAAGGTGTTGGTCTCTGCAGCATCTGGTGCCGTTGGTCAGCTTCTTGGACACTTCGCTAAGTTGTCTGGCTGTTATGTTGTTGGATCTGCCGGATCTAAAGAAAAGGTTGATCTACTGAAGAACAAATTGGGGTTTGATGAAGCTTTCAATTACAAGGAGGAGAACGATTTAGAAGCAGCTTTGAAAAGGTACTTTCCTGAAGGAATTGACGTCTACTTTGAGCATGTGGGTGGGAAATTGTTTGACGCAGTGCTGCTCAACATGAAAGAACACGGTCGCATTGCTGTCTGGCCCATCAAATACAACCTTCCCGAGCCTGAGCCACTCAAAAACCTTTTCCAAATTGCGTTGAAGCGGCTCAATGTACATGGATTTACGCATCGCGATTACGATCACATAGTACCCAAGTACTATGAGTTTGTGCTGCCTTACATCCGGGAAGGTAAAATAGTGTATGTGGAAGACATAGTTGAAGGGCTTGAGAATGGTCCAACAGCACTGGTTGGACTCTTTACTGGTCGCAACTTTGGGAAGCAAGAAGTTGCAGTTGCACCACCTCAACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

353

Amino Acids

39.5

Weight (kDa)

6.52

Isoelectric Point (pI)

28.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 11 - 118 2.8e-16 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 175 - 296 1.2e-20 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 208 - 343 6.9e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26320 AT1G26320 AT3G03080 AT3G59845 AT3G59845 AT5G16960 AT5G16960 AT5G16970 AT5G16980 AT5G16980 AT5G16980 AT5G16990 AT5G17000 AT5G17000 AT5G37940 AT5G37960 AT5G37980 AT5G38000 AT5G38000 AT5G38000
fragaria_vesca FvH4_1g00340 FvH4_1g18790 FvH4_1g23120 FvH4_1g23120
malus_domestica MD00G1045700.v1.1 MD01G1046700.v1.1 MD02G1163900.v1.1 MD15G1145600.v1.1 MD16G1282000.v1.1
prunus_persica Prupe.1G543700_v2.0.a1 Prupe.4G262000_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.6G211500_v2.0.a1 Prupe.7G269100_v2.0.a1
pyrus_communis pycom02g00040 pycom02g13040 pycom11g16640 pycom12561g00320 pycom15g13080 pycom16g25160
rosa_chinensis RchiOBHm_Chr2g0084641 RchiOBHm_Chr2g0084711 RchiOBHm_Chr2g0085361 RchiOBHm_Chr2g0085371 RchiOBHm_Chr2g0109651 RchiOBHm_Chr2g0117841 RchiOBHm_Chr6g0275751
rosa_laevigata RLG00000013436 RLG00000015629 RLG00000015633 RLG00000015680 RLG00000015681 RLG00000017753 RLG00000018376
rosa_multiflora Rmu_sc0000332.1_g000060 Rmu_sc0002352.1_g000008 Rmu_sc0002352.1_g000012 Rmu_sc0010860.1_g000005 Rmu_sc0012119.1_g000008
rosa_roxburghii Rroxscaffold_2G00126190 Rroxscaffold_2G00134060 Rroxscaffold_2G00155420 Rroxscaffold_2G00155430 Rroxscaffold_2G00155880 Rroxscaffold_2G00155890
rosa_rugosa Rorug01G0455500 Rorug01G0455600 Rorug01G0459700 Rorug01G0459800 Rorug01G0459800 Rorug01G0459900 Rorug02G0161600 Rorug02G0214400
rosa_samantha Rh2BG008800 Rh2BG282100 Rh2CG003900 Rh2CG004600 Rh2CG009500 Rh2CG009600 Rh2CG216100 Rh2CG216200 Rh2CG269100 Rh2CG269200 Rh2DG003700 Rh2DG004100 Rh2DG009900 Rh2DG219300 Rh2DG278400
rosa_wichuraiana Rw2G000300 Rw2G000330 Rw2G000770 Rw2G000780 Rw2G021390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AatII GACGTC 1 cut(s) 708
Acc36I ACCTGC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 254
AccI GTMKAC 1 cut(s) 708
AccII CGCG 1 cut(s) 879
AciI CCGC 1 cut(s) 850
AclWI GGATC 3 cut(s) 164, 586, 595
AcuI CTGAAG 3 cut(s) 131, 632, 714
AcyI GRCGYC 1 cut(s) 705
AfaI GTAC 6 cut(s) 158, 344, 686, 861, 897, 905
AfiI CCNNNNNNNGG 2 cut(s) 254, 488
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 5 cut(s) 470, 643, 679, 847, 968
AhdI GACNNNNNGTC 1 cut(s) 52
AjnI CCWGG 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 605, 637
AluBI AGCT 3 cut(s) 535, 638, 674
AluI AGCT 3 cut(s) 535, 638, 674
Alw26I GTCTC 4 cut(s) 119, 273, 486, 511
AlwI GGATC 3 cut(s) 164, 586, 595
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 2 cut(s) 283, 806
AoxI GGCC 1 cut(s) 785
ApeKI GCWGC 4 cut(s) 512, 671, 748, 919
ArsI GACNNNNNNTTYG 2 cut(s) 695, 727
AspS9I GGNCC 2 cut(s) 786, 983
AsuC2I CCSGG 1 cut(s) 932
AsuHPI GGTGA 2 cut(s) 20, 320
AvaI CYCGRG 2 cut(s) 283, 806
AvaII GGWCC 1 cut(s) 983
BanI GGYRCC 1 cut(s) 521
BbsI GAAGAC 1 cut(s) 963
BbvI GCAGC 4 cut(s) 524, 683, 735, 906
BccI CCATC 1 cut(s) 797
BceAI ACGGC 1 cut(s) 509
BciT130I CCWGG 1 cut(s) 240
BcnI CCSGG 1 cut(s) 932
BcoDI GTCTC 4 cut(s) 119, 273, 486, 511
BfmI CTRYAG 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 22
BisI GCNGC 5 cut(s) 513, 672, 749, 851, 920
BlsI GCNGC 5 cut(s) 514, 673, 750, 852, 921
BmcAI AGTACT 1 cut(s) 905
Bme1390I CCNGG 2 cut(s) 240, 932
Bme18I GGWCC 1 cut(s) 983
BmeRI GACNNNNNGTC 1 cut(s) 52
BmeT110I CYCGRG 2 cut(s) 283, 806
BmgT120I GGNCC 2 cut(s) 786, 983
BmiI GGNNCC 2 cut(s) 117, 523
BmrFI CCNGG 2 cut(s) 240, 932
BmsI GCATC 3 cut(s) 168, 524, 883
BoxI GACNNNNGTC 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 963
Bpu10I CCTNAGC 1 cut(s) 813
BpuEI CTTGAG 3 cut(s) 131, 255, 995
BpuMI CCSGG 1 cut(s) 932
BsaBI GATNNNNATC 1 cut(s) 885
BsaHI GRCGYC 1 cut(s) 705
BsaI GGTCTC 1 cut(s) 511
BsaJI CCNNGG 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 488
Bse1I ACTGG 3 cut(s) 427, 999, 1015
Bse3DI GCAATG 1 cut(s) 774
Bse8I GATNNNNATC 1 cut(s) 885
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 41
BseGI GGATG 2 cut(s) 289, 927
BseJI GATNNNNATC 1 cut(s) 885
BseLI CCNNNNNNNGG 2 cut(s) 254, 488
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 2 cut(s) 218, 804
BseNI ACTGG 3 cut(s) 427, 999, 1015
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 4 cut(s) 524, 683, 735, 906
Bsh1236I CGCG 1 cut(s) 879
Bsh1285I CGRYCG 1 cut(s) 772
BshFI GGCC 1 cut(s) 787
BshNI GGYRCC 1 cut(s) 521
BsiEI CGRYCG 1 cut(s) 772
BsiHKCI CYCGRG 2 cut(s) 283, 806
BsiSI CCGG 2 cut(s) 585, 931
BslFI GGGAC 3 cut(s) 59, 350, 389
BslI CCNNNNNNNGG 2 cut(s) 254, 488
BsmAI GTCTC 4 cut(s) 119, 273, 486, 511
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 3 cut(s) 59, 350, 389
BsnI GGCC 1 cut(s) 787
Bso31I GGTCTC 1 cut(s) 511
BsoBI CYCGRG 2 cut(s) 283, 806
Bsp1407I TGTACA 1 cut(s) 859
Bsp143I GATC 5 cut(s) 169, 578, 587, 604, 886
Bsp68I TCGCGA 1 cut(s) 879
BspACI CCGC 1 cut(s) 850
BspANI GGCC 1 cut(s) 787
BspCNI CTCAG 2 cut(s) 217, 805
BspFNI CGCG 1 cut(s) 879
BspLI GGNNCC 2 cut(s) 117, 523
BspMAI CTGCAG 1 cut(s) 514
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 3 cut(s) 164, 586, 595
BspT107I GGYRCC 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 511
BsrDI GCAATG 1 cut(s) 774
BsrGI TGTACA 1 cut(s) 859
BsrI ACTGG 3 cut(s) 427, 999, 1015
BssECI CCNNGG 1 cut(s) 41
BssMI GATC 5 cut(s) 169, 578, 587, 604, 886
BssNI GRCGYC 1 cut(s) 705
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 2 cut(s) 103, 770
Bst6I CTCTTC 1 cut(s) 333
BstACI GRCGYC 1 cut(s) 705
BstAPI GCANNNNNTGC 1 cut(s) 521
BstAUI TGTACA 1 cut(s) 859
BstDEI CTNAG 5 cut(s) 204, 483, 552, 813, 1059
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 2 cut(s) 289, 927
BstFNI CGCG 1 cut(s) 879
BstKTI GATC 5 cut(s) 172, 581, 590, 607, 889
BstMAI GTCTC 4 cut(s) 119, 273, 486, 511
BstMBI GATC 5 cut(s) 169, 578, 587, 604, 886
BstMCI CGRYCG 1 cut(s) 772
BstMWI GCNNNNNNNGC 2 cut(s) 240, 521
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 2 cut(s) 82, 722
BstPAI GACNNNNGTC 1 cut(s) 285
BstSCI CCNGG 2 cut(s) 238, 930
BstSFI CTRYAG 1 cut(s) 510
BstUI CGCG 1 cut(s) 879
BstV1I GCAGC 4 cut(s) 524, 683, 735, 906
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 2 cut(s) 578, 587
BstYI RGATCY 2 cut(s) 578, 587
BsuRI GGCC 1 cut(s) 787
BtgZI GCGATG 1 cut(s) 860
BtsCI GGATG 2 cut(s) 289, 927
BtsI GCAGTG 2 cut(s) 24, 750
BtsIMutI CAGTG 4 cut(s) 24, 396, 750, 992
BtuMI TCGCGA 1 cut(s) 879
BveI ACCTGC 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 518
Cfr13I GGNCC 2 cut(s) 786, 983
CseI GACGC 1 cut(s) 749
Csp6I GTAC 6 cut(s) 157, 343, 685, 860, 896, 904
CviAII CATG 7 cut(s) 79, 175, 333, 436, 719, 757, 863
CviQI GTAC 6 cut(s) 157, 343, 685, 860, 896, 904
DdeI CTNAG 5 cut(s) 204, 483, 552, 813, 1059
DpnI GATC 5 cut(s) 171, 580, 589, 606, 888
DpnII GATC 5 cut(s) 169, 578, 587, 604, 886
DrdI GACNNNNNNGTC 1 cut(s) 83
DriI GACNNNNNGTC 1 cut(s) 52
DseDI GACNNNNNNGTC 1 cut(s) 83
Eam1104I CTCTTC 1 cut(s) 333
Eam1105I GACNNNNNGTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 511
Eco32I GATATC 1 cut(s) 361
Eco47I GGWCC 1 cut(s) 983
Eco57I CTGAAG 3 cut(s) 131, 632, 714
Eco88I CYCGRG 2 cut(s) 283, 806
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoRII CCWGG 1 cut(s) 238
EcoRV GATATC 1 cut(s) 361
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 7 cut(s) 82, 178, 336, 439, 722, 760, 866
FaqI GGGAC 3 cut(s) 59, 350, 389
FatI CATG 7 cut(s) 78, 174, 332, 435, 718, 756, 862
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 5 cut(s) 513, 672, 749, 851, 920
FokI GGATG 2 cut(s) 276, 914
Fsp4HI GCNGC 5 cut(s) 513, 672, 749, 851, 920
GluI GCNGC 5 cut(s) 513, 672, 749, 851, 920
HaeIII GGCC 1 cut(s) 787
HapII CCGG 2 cut(s) 585, 931
HgaI GACGC 1 cut(s) 749
Hin1I GRCGYC 1 cut(s) 705
Hin1II CATG 7 cut(s) 82, 178, 336, 439, 722, 760, 866
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 5 cut(s) 71, 281, 286, 462, 1002
HpaI GTTAAC 1 cut(s) 327
HpaII CCGG 2 cut(s) 585, 931
HphI GGTGA 2 cut(s) 20, 320
Hpy166II GTNNAC 3 cut(s) 280, 327, 709
Hpy188I TCNGA 2 cut(s) 76, 111
Hpy188III TCNNGA 5 cut(s) 148, 293, 692, 806, 878
Hpy8I GTNNAC 3 cut(s) 280, 327, 709
HpyAV CCTTC 5 cut(s) 106, 689, 812, 929, 962
HpyCH4III ACNGT 2 cut(s) 103, 770
HpyCH4IV ACGT 1 cut(s) 705
HpyCH4V TGCA 5 cut(s) 181, 234, 512, 1040, 1046
HpyF10VI GCNNNNNNNGC 2 cut(s) 240, 521
HpyF3I CTNAG 5 cut(s) 204, 483, 552, 813, 1059
HpySE526I ACGT 1 cut(s) 705
Hsp92I GRCGYC 1 cut(s) 705
Hsp92II CATG 7 cut(s) 82, 178, 336, 439, 722, 760, 866
KspAI GTTAAC 1 cut(s) 327
Kzo9I GATC 5 cut(s) 169, 578, 587, 604, 886
Lsp1109I GCAGC 4 cut(s) 524, 683, 735, 906
LweI GCATC 3 cut(s) 168, 524, 883
MaeII ACGT 1 cut(s) 705
MaeIII GTNAC 1 cut(s) 85
MalI GATC 5 cut(s) 171, 580, 589, 606, 888
MboI GATC 5 cut(s) 169, 578, 587, 604, 886
MboII GAAGA 3 cut(s) 350, 625, 968
MflI RGATCY 2 cut(s) 578, 587
MluCI AATT 7 cut(s) 182, 249, 620, 643, 699, 731, 837
MlyI GAGTC 4 cut(s) 80, 275, 295, 996
MmeI TCCRAC 3 cut(s) 556, 979, 1010
MnlI CCTC 9 cut(s) 15, 36, 197, 359, 385, 417, 489, 646, 1062
MseI TTAA 2 cut(s) 326, 384
MslI CAYNNNNRTG 2 cut(s) 399, 723
MspI CCGG 2 cut(s) 585, 931
MspR9I CCNGG 2 cut(s) 240, 932
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 1 cut(s) 879
MwoI GCNNNNNNNGC 2 cut(s) 240, 521
NciI CCSGG 1 cut(s) 932
NdeII GATC 5 cut(s) 169, 578, 587, 604, 886
NlaIII CATG 7 cut(s) 82, 178, 336, 439, 722, 760, 866
NlaIV GGNNCC 2 cut(s) 117, 523
NmuCI GTSAC 1 cut(s) 85
NruI TCGCGA 1 cut(s) 879
NspI RCATGY 2 cut(s) 82, 722
PaeR7I CTCGAG 1 cut(s) 283
PciI ACATGT 1 cut(s) 78
PfeI GAWTC 1 cut(s) 462
PflMI CCANNNNNTGG 1 cut(s) 254
PfoI TCCNGGA 1 cut(s) 930
PkrI GCNGC 5 cut(s) 514, 673, 750, 852, 921
PleI GAGTC 4 cut(s) 79, 275, 294, 996
PpsI GAGTC 4 cut(s) 79, 275, 294, 996
PscI ACATGT 1 cut(s) 78
PshAI GACNNNNGTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 117, 523
PspPI GGNCC 2 cut(s) 786, 983
PspXI VCTCGAGB 1 cut(s) 283
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 2 cut(s) 578, 587
RruI TCGCGA 1 cut(s) 879
RsaI GTAC 6 cut(s) 158, 344, 686, 861, 897, 905
RsaNI GTAC 6 cut(s) 157, 343, 685, 860, 896, 904
RseI CAYNNNNRTG 2 cut(s) 399, 723
SaqAI TTAA 2 cut(s) 326, 384
SatI GCNGC 5 cut(s) 513, 672, 749, 851, 920
Sau3AI GATC 5 cut(s) 169, 578, 587, 604, 886
Sau96I GGNCC 2 cut(s) 786, 983
ScaI AGTACT 1 cut(s) 905
SchI GAGTC 4 cut(s) 80, 275, 295, 996
ScrFI CCNGG 2 cut(s) 240, 932
SfaNI GCATC 3 cut(s) 168, 524, 883
SfcI CTRYAG 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 283
SinI GGWCC 1 cut(s) 983
SlaI CTCGAG 1 cut(s) 283
SmiMI CAYNNNNRTG 2 cut(s) 399, 723
SmlI CTYRAG 4 cut(s) 146, 270, 283, 974
SmoI CTYRAG 4 cut(s) 146, 270, 283, 974
Sse9I AATT 7 cut(s) 182, 249, 620, 643, 699, 731, 837
SsiI CCGC 1 cut(s) 850
StyD4I CCNGG 2 cut(s) 238, 930
TaaI ACNGT 2 cut(s) 103, 770
TaiI ACGT 1 cut(s) 708
TaqI TCGA 1 cut(s) 284
TasI AATT 7 cut(s) 182, 249, 620, 643, 699, 731, 837
TatI WGTACW 4 cut(s) 156, 342, 859, 903
TauI GCSGC 1 cut(s) 853
TfiI GAWTC 1 cut(s) 462
Tru1I TTAA 2 cut(s) 326, 384
Tru9I TTAA 2 cut(s) 326, 384
TscAI CASTG 4 cut(s) 24, 403, 750, 999
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 4 cut(s) 512, 671, 748, 919
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 648, 773
TspRI CASTG 4 cut(s) 24, 403, 750, 999
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 983
XagI CCTNNNNNAGG 1 cut(s) 486
XceI RCATGY 2 cut(s) 82, 722
XhoI CTCGAG 1 cut(s) 283
XmiI GTMKAC 1 cut(s) 708
ZraI GACGTC 1 cut(s) 706
ZrmI AGTACT 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.