2-alkenal reductase (NADP( )-dependent)-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 13 |
| Acc36I | ACCTGC | 1 cut(s) | 13 |
| AciI | CCGC | 3 cut(s) | 90, 140, 468 |
| AclWI | GGATC | 1 cut(s) | 191 |
| AcsI | RAATTY | 2 cut(s) | 444, 926 |
| AcuI | CTGAAG | 1 cut(s) | 858 |
| AfaI | GTAC | 3 cut(s) | 159, 371, 821 |
| AfeI | AGCGCT | 1 cut(s) | 1017 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 506 |
| AflII | CTTAAG | 1 cut(s) | 173 |
| AgsI | TTSAA | 8 cut(s) | 106, 403, 631, 685, 697, 734, 992, 1033 |
| AhlI | ACTAGT | 1 cut(s) | 163 |
| AjnI | CCWGG | 2 cut(s) | 265, 457 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 882, 914 |
| AluBI | AGCT | 5 cut(s) | 109, 134, 536, 553, 862 |
| AluI | AGCT | 5 cut(s) | 109, 134, 536, 553, 862 |
| Alw21I | GWGCWC | 1 cut(s) | 768 |
| Alw26I | GTCTC | 4 cut(s) | 113, 330, 772, 1072 |
| AlwI | GGATC | 1 cut(s) | 191 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 122, 536, 902 |
| Aor51HI | AGCGCT | 1 cut(s) | 1017 |
| AoxI | GGCC | 2 cut(s) | 51, 994 |
| ApeKI | GCWGC | 4 cut(s) | 530, 533, 859, 1013 |
| ApoI | RAATTY | 2 cut(s) | 444, 926 |
| AseI | ATTAAT | 1 cut(s) | 849 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 638, 811 |
| AspLEI | GCGC | 3 cut(s) | 792, 876, 1018 |
| AspS9I | GGNCC | 2 cut(s) | 52, 1007 |
| AsuC2I | CCSGG | 1 cut(s) | 710 |
| AsuHPI | GGTGA | 2 cut(s) | 37, 905 |
| AvaII | GGWCC | 1 cut(s) | 1007 |
| BanII | GRGCYC | 1 cut(s) | 237 |
| BbsI | GAAGAC | 3 cut(s) | 511, 987, 1057 |
| Bbv12I | GWGCWC | 1 cut(s) | 768 |
| BbvI | GCAGC | 4 cut(s) | 542, 545, 871, 1025 |
| BccI | CCATC | 2 cut(s) | 253, 571 |
| BciT130I | CCWGG | 2 cut(s) | 267, 459 |
| BclI | TGATCA | 3 cut(s) | 81, 124, 904 |
| BcnI | CCSGG | 1 cut(s) | 710 |
| BcoDI | GTCTC | 4 cut(s) | 113, 330, 772, 1072 |
| BcuI | ACTAGT | 1 cut(s) | 163 |
| BfaI | CTAG | 2 cut(s) | 164, 312 |
| BfmI | CTRYAG | 1 cut(s) | 114 |
| BfoI | RGCGCY | 1 cut(s) | 1019 |
| BfrI | CTTAAG | 1 cut(s) | 173 |
| BfuAI | ACCTGC | 1 cut(s) | 13 |
| BisI | GCNGC | 4 cut(s) | 531, 534, 860, 1014 |
| BlpI | GCTNAGC | 1 cut(s) | 236 |
| BlsI | GCNGC | 4 cut(s) | 532, 535, 861, 1015 |
| Bme1390I | CCNGG | 3 cut(s) | 267, 459, 710 |
| Bme18I | GGWCC | 1 cut(s) | 1007 |
| BmgT120I | GGNCC | 2 cut(s) | 52, 1007 |
| BmrFI | CCNGG | 3 cut(s) | 267, 459, 710 |
| BmsI | GCATC | 3 cut(s) | 127, 748, 846 |
| BpiI | GAAGAC | 3 cut(s) | 511, 987, 1057 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 236 |
| BpuEI | CTTGAG | 1 cut(s) | 1019 |
| BpuMI | CCSGG | 1 cut(s) | 710 |
| BsaBI | GATNNNNATC | 1 cut(s) | 909 |
| BsaJI | CCNNGG | 3 cut(s) | 229, 784, 886 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 327, 357, 501, 531 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 506 |
| Bse1I | ACTGG | 1 cut(s) | 445 |
| Bse8I | GATNNNNATC | 1 cut(s) | 909 |
| BseBI | CCWGG | 2 cut(s) | 267, 459 |
| BseDI | CCNNGG | 3 cut(s) | 229, 784, 886 |
| BseGI | GGATG | 2 cut(s) | 146, 763 |
| BseJI | GATNNNNATC | 1 cut(s) | 909 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 506 |
| BseMII | CTCAG | 2 cut(s) | 23, 250 |
| BseNI | ACTGG | 1 cut(s) | 445 |
| BseXI | GCAGC | 4 cut(s) | 542, 545, 871, 1025 |
| BshFI | GGCC | 2 cut(s) | 53, 996 |
| BsiHKAI | GWGCWC | 1 cut(s) | 768 |
| BsiSI | CCGG | 1 cut(s) | 710 |
| BslFI | GGGAC | 2 cut(s) | 227, 364 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 506 |
| BsmAI | GTCTC | 4 cut(s) | 113, 330, 772, 1072 |
| BsmFI | GGGAC | 2 cut(s) | 227, 364 |
| BsmI | GAATGC | 1 cut(s) | 876 |
| BsnI | GGCC | 2 cut(s) | 53, 996 |
| Bsp1286I | GDGCHC | 2 cut(s) | 237, 768 |
| Bsp143I | GATC | 5 cut(s) | 81, 124, 196, 622, 904 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 236 |
| Bsp19I | CCATGG | 2 cut(s) | 784, 886 |
| BspACI | CCGC | 3 cut(s) | 90, 140, 468 |
| BspANI | GGCC | 2 cut(s) | 53, 996 |
| BspCNI | CTCAG | 2 cut(s) | 24, 249 |
| BspHI | TCATGA | 1 cut(s) | 1072 |
| BspMAI | CTGCAG | 1 cut(s) | 118 |
| BspMI | ACCTGC | 1 cut(s) | 13 |
| BspPI | GGATC | 1 cut(s) | 191 |
| BspTI | CTTAAG | 1 cut(s) | 173 |
| BsrI | ACTGG | 1 cut(s) | 445 |
| BssECI | CCNNGG | 3 cut(s) | 229, 784, 886 |
| BssMI | GATC | 5 cut(s) | 81, 124, 196, 622, 904 |
| BssT1I | CCWWGG | 3 cut(s) | 229, 784, 886 |
| Bst2UI | CCWGG | 2 cut(s) | 267, 459 |
| Bst4CI | ACNGT | 2 cut(s) | 374, 819 |
| Bst6I | CTCTTC | 2 cut(s) | 360, 404 |
| BstAFI | CTTAAG | 1 cut(s) | 173 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 539 |
| BstC8I | GCNNGC | 1 cut(s) | 132 |
| BstDEI | CTNAG | 4 cut(s) | 32, 236, 340, 501 |
| BstDSI | CCRYGG | 2 cut(s) | 784, 886 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 504 |
| BstF5I | GGATG | 2 cut(s) | 146, 763 |
| BstH2I | RGCGCY | 1 cut(s) | 1019 |
| BstHHI | GCGC | 3 cut(s) | 792, 876, 1018 |
| BstKTI | GATC | 5 cut(s) | 84, 127, 199, 625, 907 |
| BstMAI | GTCTC | 4 cut(s) | 113, 330, 772, 1072 |
| BstMBI | GATC | 5 cut(s) | 81, 124, 196, 622, 904 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 539 |
| BstNI | CCWGG | 2 cut(s) | 267, 459 |
| BstSCI | CCNGG | 3 cut(s) | 265, 457, 708 |
| BstSFI | CTRYAG | 1 cut(s) | 114 |
| BstV1I | GCAGC | 4 cut(s) | 542, 545, 871, 1025 |
| BstV2I | GAAGAC | 3 cut(s) | 511, 987, 1057 |
| BsuRI | GGCC | 2 cut(s) | 53, 996 |
| BtgI | CCRYGG | 2 cut(s) | 784, 886 |
| BtsCI | GGATG | 2 cut(s) | 146, 763 |
| BveI | ACCTGC | 1 cut(s) | 13 |
| Cac8I | GCNNGC | 1 cut(s) | 132 |
| CaiI | CAGNNNCTG | 3 cut(s) | 122, 536, 902 |
| CciI | TCATGA | 1 cut(s) | 1072 |
| CfoI | GCGC | 3 cut(s) | 792, 876, 1018 |
| Cfr13I | GGNCC | 2 cut(s) | 52, 1007 |
| CseI | GACGC | 2 cut(s) | 214, 229 |
| Csp6I | GTAC | 3 cut(s) | 158, 370, 820 |
| CviAII | CATG | 4 cut(s) | 775, 785, 887, 1073 |
| CviQI | GTAC | 3 cut(s) | 158, 370, 820 |
| DdeI | CTNAG | 4 cut(s) | 32, 236, 340, 501 |
| DpnI | GATC | 5 cut(s) | 83, 126, 198, 624, 906 |
| DpnII | GATC | 5 cut(s) | 81, 124, 196, 622, 904 |
| DraI | TTTAAA | 1 cut(s) | 327 |
| Eam1104I | CTCTTC | 2 cut(s) | 360, 404 |
| EarI | CTCTTC | 2 cut(s) | 360, 404 |
| Eco130I | CCWWGG | 3 cut(s) | 229, 784, 886 |
| Eco147I | AGGCCT | 1 cut(s) | 996 |
| Eco24I | GRGCYC | 1 cut(s) | 237 |
| Eco47I | GGWCC | 1 cut(s) | 1007 |
| Eco47III | AGCGCT | 1 cut(s) | 1017 |
| Eco57I | CTGAAG | 1 cut(s) | 858 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 504 |
| EcoO109I | RGGNCCY | 1 cut(s) | 52 |
| EcoRII | CCWGG | 2 cut(s) | 265, 457 |
| EcoT14I | CCWWGG | 3 cut(s) | 229, 784, 886 |
| EcoT38I | GRGCYC | 1 cut(s) | 237 |
| ErhI | CCWWGG | 3 cut(s) | 229, 784, 886 |
| FaeI | CATG | 4 cut(s) | 778, 788, 890, 1076 |
| FaqI | GGGAC | 2 cut(s) | 227, 364 |
| FatI | CATG | 4 cut(s) | 774, 784, 886, 1072 |
| FbaI | TGATCA | 3 cut(s) | 81, 124, 904 |
| Fnu4HI | GCNGC | 4 cut(s) | 531, 534, 860, 1014 |
| FokI | GGATG | 2 cut(s) | 133, 770 |
| FriOI | GRGCYC | 1 cut(s) | 237 |
| Fsp4HI | GCNGC | 4 cut(s) | 531, 534, 860, 1014 |
| FspBI | CTAG | 2 cut(s) | 164, 312 |
| GlaI | GCGC | 3 cut(s) | 791, 875, 1017 |
| GluI | GCNGC | 4 cut(s) | 531, 534, 860, 1014 |
| HaeII | RGCGCY | 1 cut(s) | 1019 |
| HaeIII | GGCC | 2 cut(s) | 53, 996 |
| HapII | CCGG | 1 cut(s) | 710 |
| HgaI | GACGC | 2 cut(s) | 214, 229 |
| HhaI | GCGC | 3 cut(s) | 792, 876, 1018 |
| Hin1II | CATG | 4 cut(s) | 778, 788, 890, 1076 |
| Hin6I | GCGC | 3 cut(s) | 790, 874, 1016 |
| HinP1I | GCGC | 3 cut(s) | 790, 874, 1016 |
| HindIII | AAGCTT | 1 cut(s) | 107 |
| HinfI | GANTC | 5 cut(s) | 65, 210, 308, 316, 780 |
| HpaII | CCGG | 1 cut(s) | 710 |
| HphI | GGTGA | 2 cut(s) | 37, 905 |
| Hpy188I | TCNGA | 2 cut(s) | 70, 644 |
| Hpy188III | TCNNGA | 4 cut(s) | 320, 827, 902, 1073 |
| HpyAV | CCTTC | 4 cut(s) | 65, 707, 833, 986 |
| HpyCH4III | ACNGT | 2 cut(s) | 374, 819 |
| HpyCH4IV | ACGT | 1 cut(s) | 516 |
| HpyCH4V | TGCA | 3 cut(s) | 116, 569, 859 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 539 |
| HpyF3I | CTNAG | 4 cut(s) | 32, 236, 340, 501 |
| HpySE526I | ACGT | 1 cut(s) | 516 |
| Hsp92II | CATG | 4 cut(s) | 778, 788, 890, 1076 |
| HspAI | GCGC | 3 cut(s) | 790, 874, 1016 |
| Ksp22I | TGATCA | 3 cut(s) | 81, 124, 904 |
| Kzo9I | GATC | 5 cut(s) | 81, 124, 196, 622, 904 |
| LmnI | GCTCC | 1 cut(s) | 771 |
| Lsp1109I | GCAGC | 4 cut(s) | 542, 545, 871, 1025 |
| LweI | GCATC | 3 cut(s) | 127, 748, 846 |
| MaeI | CTAG | 2 cut(s) | 164, 312 |
| MaeII | ACGT | 1 cut(s) | 516 |
| MaeIII | GTNAC | 4 cut(s) | 14, 142, 608, 1040 |
| MalI | GATC | 5 cut(s) | 83, 126, 198, 624, 906 |
| MboI | GATC | 5 cut(s) | 81, 124, 196, 622, 904 |
| MboII | GAAGA | 8 cut(s) | 377, 391, 511, 643, 683, 976, 992, 1062 |
| MfeI | CAATTG | 1 cut(s) | 358 |
| MhlI | GDGCHC | 2 cut(s) | 237, 768 |
| MluCI | AATT | 7 cut(s) | 358, 444, 563, 572, 846, 850, 926 |
| MlyI | GAGTC | 4 cut(s) | 74, 204, 302, 774 |
| MmeI | TCCRAC | 3 cut(s) | 538, 574, 1003 |
| MnlI | CCTC | 8 cut(s) | 27, 160, 191, 265, 407, 646, 926, 957 |
| MroXI | GAANNNNTTC | 2 cut(s) | 638, 811 |
| MseI | TTAA | 5 cut(s) | 174, 326, 660, 771, 849 |
| MslI | CAYNNNNRTG | 1 cut(s) | 285 |
| MspA1I | CMGCKG | 2 cut(s) | 134, 536 |
| MspCI | CTTAAG | 1 cut(s) | 173 |
| MspI | CCGG | 1 cut(s) | 710 |
| MspR9I | CCNGG | 3 cut(s) | 267, 459, 710 |
| MunI | CAATTG | 1 cut(s) | 358 |
| Mva1269I | GAATGC | 1 cut(s) | 876 |
| MvaI | CCWGG | 2 cut(s) | 267, 459 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 539 |
| NciI | CCSGG | 1 cut(s) | 710 |
| NcoI | CCATGG | 2 cut(s) | 784, 886 |
| NdeII | GATC | 5 cut(s) | 81, 124, 196, 622, 904 |
| NlaIII | CATG | 4 cut(s) | 778, 788, 890, 1076 |
| PagI | TCATGA | 1 cut(s) | 1072 |
| PaqCI | CACCTGC | 1 cut(s) | 13 |
| PceI | AGGCCT | 1 cut(s) | 996 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 618, 648 |
| PctI | GAATGC | 1 cut(s) | 876 |
| PdmI | GAANNNNTTC | 2 cut(s) | 638, 811 |
| PfeI | GAWTC | 1 cut(s) | 316 |
| PfoI | TCCNGGA | 1 cut(s) | 708 |
| PkrI | GCNGC | 4 cut(s) | 532, 535, 861, 1015 |
| PleI | GAGTC | 4 cut(s) | 73, 204, 302, 774 |
| PpsI | GAGTC | 4 cut(s) | 73, 204, 302, 774 |
| PshBI | ATTAAT | 1 cut(s) | 849 |
| Psp6I | CCWGG | 2 cut(s) | 265, 457 |
| PspGI | CCWGG | 2 cut(s) | 265, 457 |
| PspPI | GGNCC | 2 cut(s) | 52, 1007 |
| PstI | CTGCAG | 1 cut(s) | 118 |
| PstNI | CAGNNNCTG | 3 cut(s) | 122, 536, 902 |
| PvuII | CAGCTG | 2 cut(s) | 134, 536 |
| RsaI | GTAC | 3 cut(s) | 159, 371, 821 |
| RsaNI | GTAC | 3 cut(s) | 158, 370, 820 |
| RseI | CAYNNNNRTG | 1 cut(s) | 285 |
| SaqAI | TTAA | 5 cut(s) | 174, 326, 660, 771, 849 |
| SatI | GCNGC | 4 cut(s) | 531, 534, 860, 1014 |
| Sau3AI | GATC | 5 cut(s) | 81, 124, 196, 622, 904 |
| Sau96I | GGNCC | 2 cut(s) | 52, 1007 |
| SchI | GAGTC | 4 cut(s) | 74, 204, 302, 774 |
| ScrFI | CCNGG | 3 cut(s) | 267, 459, 710 |
| SduI | GDGCHC | 2 cut(s) | 237, 768 |
| SfaNI | GCATC | 3 cut(s) | 127, 748, 846 |
| SfcI | CTRYAG | 1 cut(s) | 114 |
| SinI | GGWCC | 1 cut(s) | 1007 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 285 |
| SmlI | CTYRAG | 2 cut(s) | 173, 998 |
| SmoI | CTYRAG | 2 cut(s) | 173, 998 |
| SpeI | ACTAGT | 1 cut(s) | 163 |
| Sse9I | AATT | 7 cut(s) | 358, 444, 563, 572, 846, 850, 926 |
| SseBI | AGGCCT | 1 cut(s) | 996 |
| SsiI | CCGC | 3 cut(s) | 90, 140, 468 |
| SspMI | CTAG | 2 cut(s) | 164, 312 |
| StuI | AGGCCT | 1 cut(s) | 996 |
| StyD4I | CCNGG | 3 cut(s) | 265, 457, 708 |
| StyI | CCWWGG | 3 cut(s) | 229, 784, 886 |
| TaaI | ACNGT | 2 cut(s) | 374, 819 |
| TaiI | ACGT | 1 cut(s) | 519 |
| TaqI | TCGA | 1 cut(s) | 621 |
| TasI | AATT | 7 cut(s) | 358, 444, 563, 572, 846, 850, 926 |
| TatI | WGTACW | 2 cut(s) | 369, 819 |
| TfiI | GAWTC | 1 cut(s) | 316 |
| Tru1I | TTAA | 5 cut(s) | 174, 326, 660, 771, 849 |
| Tru9I | TTAA | 5 cut(s) | 174, 326, 660, 771, 849 |
| TseI | GCWGC | 4 cut(s) | 530, 533, 859, 1013 |
| TspDTI | ATGAA | 1 cut(s) | 511 |
| TspGWI | ACGGA | 1 cut(s) | 297 |
| Vha464I | CTTAAG | 1 cut(s) | 173 |
| VpaK11BI | GGWCC | 1 cut(s) | 1007 |
| VspI | ATTAAT | 1 cut(s) | 849 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 504 |
| XapI | RAATTY | 2 cut(s) | 444, 926 |
| XmnI | GAANNNNTTC | 2 cut(s) | 638, 811 |
| XspI | CTAG | 2 cut(s) | 164, 312 |