FvH4_1g23120

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000431
Physical Location & Seq
Reverse (-)
14966374 .. 14969202
2829 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23120.t2

Sequence Viewer

Length: 1062 bp
ATGACAGAAGTGAATAACAAACAGGTGATATTGAGGAACTATATCACCGCCTTCCCGAAAGATACAGACTTATACGTGACCAGCTCTTCTGTCAGTACCAAGTTCAAGCTTCCTACTGACACTGCTACTGCTACTTCAAATTCAAAGGTACTTGCAGTTATAGTGAAGAACCTCTACCTCTCTTGCGACCCCTACCAGCGGCTTTACATGAAAAAGATCGCTGGACTCAGCACCTATACACCCGGCTCACCAATATTTGGGTATGGAGTGGCTGAAGTTTTGGACTCGCAGCACCCAGATTTTAAGGCAGGTGACTTAGTTTGGGGGACTACCAATTGGGAACAATTCAGCCTTCTAACAACACCCAAAAACCTCATCAAAATCCAGCATACTGAGACTGATGTCCCTCTTTCATATTATGCTGGAATTCTCGGCATTACTGGGTTGACCTCGTACGTAGGTTTTTACGAGGTTTGTTCACCCAAGGAAGGAGAACATATCTTCATTTCAGCAGCAGCTGGTGCTGTTGGTCAGATTGTTGGGCAACTCGCAAAATTGTTGGGATGTTATGTTGTTGGAAGTGCCGGTACTAAGGAGAAGGTTGATCTGTTGAAGAATAAATTGGGGTTCGACGAGGCTTTCAACTACAAAGAAGAGAATGACTTGGATCTGGCTTTGAAAAGGTACTTTCCAGAAGGCATTGATATATATTTTGATAATGTTGGGGGCAAAATGTTGGATGCAGTCCTCCTAAACATGAGAGTCCATGGACGAATTGCTGGTTGTGGAATGATCTCGCAATACAATCTTGCTCAGCCGGAAGGGGTAACAAATTTGATGCACCTCGTAGTCAAGCGTGTAAGCTACCATGGATTTACAGTCCGCGATTACTATCATGTCTACCCCAAGTTCTTAGATTTCATTCTGCCTTACTTACGACAAGGAAAGATCGTTTACGTGGAAGACATGGTTAAAGGCCTCGAGGTTGCACCAGCAGCACTGTTCGGACTTTTTAAGGGTCACAACATTGGAAAACAAGTAGTTGCAGTTGATCATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

354

Amino Acids

39.12

Weight (kDa)

6.76

Isoelectric Point (pI)

27.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 5 - 48 4.6e-07 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 96 - 229 9.9e-24 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 129 - 269 8.6e-09 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26320 AT1G26320 AT3G03080 AT3G59845 AT3G59845 AT5G16960 AT5G16960 AT5G16970 AT5G16980 AT5G16980 AT5G16980 AT5G16990 AT5G17000 AT5G17000 AT5G37940 AT5G37960 AT5G37980 AT5G38000 AT5G38000 AT5G38000
fragaria_vesca FvH4_1g00340 FvH4_1g18790 FvH4_1g23120 FvH4_1g23120
malus_domestica MD00G1045700.v1.1 MD01G1046700.v1.1 MD02G1163900.v1.1 MD15G1145600.v1.1 MD16G1282000.v1.1
prunus_persica Prupe.1G543700_v2.0.a1 Prupe.4G262000_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.6G211500_v2.0.a1 Prupe.7G269100_v2.0.a1
pyrus_communis pycom02g00040 pycom02g13040 pycom11g16640 pycom12561g00320 pycom15g13080 pycom16g25160
rosa_chinensis RchiOBHm_Chr2g0084641 RchiOBHm_Chr2g0084711 RchiOBHm_Chr2g0085361 RchiOBHm_Chr2g0085371 RchiOBHm_Chr2g0109651 RchiOBHm_Chr2g0117841 RchiOBHm_Chr6g0275751
rosa_laevigata RLG00000013436 RLG00000015629 RLG00000015633 RLG00000015680 RLG00000015681 RLG00000017753 RLG00000018376
rosa_multiflora Rmu_sc0000332.1_g000060 Rmu_sc0002352.1_g000008 Rmu_sc0002352.1_g000012 Rmu_sc0010860.1_g000005 Rmu_sc0012119.1_g000008
rosa_roxburghii Rroxscaffold_2G00126190 Rroxscaffold_2G00134060 Rroxscaffold_2G00155420 Rroxscaffold_2G00155430 Rroxscaffold_2G00155880 Rroxscaffold_2G00155890
rosa_rugosa Rorug01G0455500 Rorug01G0455600 Rorug01G0459700 Rorug01G0459800 Rorug01G0459800 Rorug01G0459900 Rorug02G0161600 Rorug02G0214400
rosa_samantha Rh2BG008800 Rh2BG282100 Rh2CG003900 Rh2CG004600 Rh2CG009500 Rh2CG009600 Rh2CG216100 Rh2CG216200 Rh2CG269100 Rh2CG269200 Rh2DG003700 Rh2DG004100 Rh2DG009900 Rh2DG219300 Rh2DG278400
rosa_wichuraiana Rw2G000300 Rw2G000330 Rw2G000770 Rw2G000780 Rw2G021390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 299
AbsI CCTCGAGG 1 cut(s) 980
Acc36I ACCTGC 1 cut(s) 299
AccB7I CCANNNNNTGG 1 cut(s) 257
AccI GTMKAC 1 cut(s) 900
AccII CGCG 1 cut(s) 885
AciI CCGC 3 cut(s) 48, 199, 883
AclWI GGATC 1 cut(s) 675
AcsI RAATTY 3 cut(s) 139, 426, 832
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 5 cut(s) 97, 150, 455, 589, 686
AfiI CCNNNNNNNGG 3 cut(s) 198, 257, 488
AgsI TTSAA 6 cut(s) 106, 138, 144, 613, 643, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 605, 637
AluBI AGCT 4 cut(s) 84, 109, 518, 864
AluI AGCT 4 cut(s) 84, 109, 518, 864
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 1 cut(s) 675
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 980
AoxI GGCC 1 cut(s) 976
ApeKI GCWGC 4 cut(s) 289, 512, 515, 995
ApoI RAATTY 3 cut(s) 139, 426, 832
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 5 cut(s) 37, 37, 240, 323, 471
AvaI CYCGRG 1 cut(s) 980
BarI GAAGNNNNNNTAC 4 cut(s) 158, 190, 571, 603
BbsI GAAGAC 1 cut(s) 969
BbvI GCAGC 4 cut(s) 301, 524, 527, 1007
BclI TGATCA 1 cut(s) 1051
BcnI CCSGG 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 389
BfuAI ACCTGC 1 cut(s) 299
BisI GCNGC 5 cut(s) 200, 290, 513, 516, 996
BlpI GCTNAGC 1 cut(s) 813
BlsI GCNGC 5 cut(s) 201, 291, 514, 517, 997
Bme1390I CCNGG 1 cut(s) 243
BmeT110I CYCGRG 1 cut(s) 980
BmrFI CCNGG 1 cut(s) 243
BmrI ACTGGG 1 cut(s) 450
BmsI GCATC 2 cut(s) 730, 828
BmuI ACTGGG 1 cut(s) 450
BoxI GACNNNNGTC 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 969
Bpu1102I GCTNAGC 1 cut(s) 813
BpuMI CCSGG 1 cut(s) 243
BsaAI YACGTR 3 cut(s) 76, 457, 958
BsaBI GATNNNNATC 1 cut(s) 891
BsaJI CCNNGG 3 cut(s) 483, 766, 868
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 257, 488
Bse118I RCCGGY 1 cut(s) 584
Bse1I ACTGG 1 cut(s) 445
Bse8I GATNNNNATC 1 cut(s) 891
BseDI CCNNGG 3 cut(s) 483, 766, 868
BseGI GGATG 2 cut(s) 569, 745
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 3 cut(s) 198, 257, 488
BseMII CTCAG 3 cut(s) 241, 384, 827
BseNI ACTGG 1 cut(s) 445
BseXI GCAGC 4 cut(s) 301, 524, 527, 1007
Bsh1236I CGCG 1 cut(s) 885
BshFI GGCC 1 cut(s) 978
BsiHKCI CYCGRG 1 cut(s) 980
BsiSI CCGG 3 cut(s) 243, 585, 818
BsiWI CGTACG 1 cut(s) 453
BslFI GGGAC 2 cut(s) 340, 389
BslI CCNNNNNNNGG 3 cut(s) 198, 257, 488
BsmAI GTCTC 1 cut(s) 389
BsmFI GGGAC 2 cut(s) 340, 389
BsnI GGCC 1 cut(s) 978
BsoBI CYCGRG 1 cut(s) 980
Bsp143I GATC 6 cut(s) 216, 604, 667, 792, 948, 1051
Bsp1720I GCTNAGC 1 cut(s) 813
Bsp19I CCATGG 2 cut(s) 766, 868
BspACI CCGC 3 cut(s) 48, 199, 883
BspANI GGCC 1 cut(s) 978
BspCNI CTCAG 3 cut(s) 240, 385, 826
BspFNI CGCG 1 cut(s) 885
BspHI TCATGA 1 cut(s) 1054
BspMI ACCTGC 1 cut(s) 299
BspPI GGATC 1 cut(s) 675
BspQI GCTCTTC 1 cut(s) 91
BsrFI RCCGGY 1 cut(s) 584
BsrI ACTGG 1 cut(s) 445
BssAI RCCGGY 1 cut(s) 584
BssECI CCNNGG 3 cut(s) 483, 766, 868
BssMI GATC 6 cut(s) 216, 604, 667, 792, 948, 1051
BssT1I CCWWGG 3 cut(s) 483, 766, 868
Bst4CI ACNGT 2 cut(s) 880, 1002
Bst6I CTCTTC 2 cut(s) 91, 648
BstAPI GCANNNNNTGC 1 cut(s) 521
BstBAI YACGTR 3 cut(s) 76, 457, 958
BstDEI CTNAG 6 cut(s) 227, 316, 393, 591, 813, 913
BstDSI CCRYGG 2 cut(s) 766, 868
BstF5I GGATG 2 cut(s) 569, 745
BstFNI CGCG 1 cut(s) 885
BstKTI GATC 6 cut(s) 219, 607, 670, 795, 951, 1054
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 6 cut(s) 216, 604, 667, 792, 948, 1051
BstMWI GCNNNNNNNGC 2 cut(s) 521, 995
BstPAI GACNNNNGTC 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 241
BstSNI TACGTA 1 cut(s) 457
BstUI CGCG 1 cut(s) 885
BstV1I GCAGC 4 cut(s) 301, 524, 527, 1007
BstV2I GAAGAC 1 cut(s) 969
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BsuRI GGCC 1 cut(s) 978
BtgI CCRYGG 2 cut(s) 766, 868
BtsCI GGATG 2 cut(s) 569, 745
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 2 cut(s) 120, 998
BveI ACCTGC 1 cut(s) 299
CaiI CAGNNNCTG 1 cut(s) 518
CciI TCATGA 1 cut(s) 1054
Cfr10I RCCGGY 1 cut(s) 584
Csp6I GTAC 5 cut(s) 96, 149, 454, 588, 685
CviAII CATG 7 cut(s) 208, 757, 767, 869, 896, 967, 1055
CviQI GTAC 5 cut(s) 96, 149, 454, 588, 685
DdeI CTNAG 6 cut(s) 227, 316, 393, 591, 813, 913
DpnI GATC 6 cut(s) 218, 606, 669, 794, 950, 1053
DpnII GATC 6 cut(s) 216, 604, 667, 792, 948, 1051
Eam1104I CTCTTC 2 cut(s) 91, 648
EarI CTCTTC 2 cut(s) 91, 648
Eco105I TACGTA 1 cut(s) 457
Eco130I CCWWGG 3 cut(s) 483, 766, 868
Eco147I AGGCCT 1 cut(s) 978
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 980
EcoRI GAATTC 1 cut(s) 426
EcoT14I CCWWGG 3 cut(s) 483, 766, 868
ErhI CCWWGG 3 cut(s) 483, 766, 868
FaeI CATG 7 cut(s) 211, 760, 770, 872, 899, 970, 1058
FaqI GGGAC 2 cut(s) 340, 389
FatI CATG 7 cut(s) 207, 756, 766, 868, 895, 966, 1054
FbaI TGATCA 1 cut(s) 1051
FblI GTMKAC 1 cut(s) 900
Fnu4HI GCNGC 5 cut(s) 200, 290, 513, 516, 996
FokI GGATG 2 cut(s) 576, 752
Fsp4HI GCNGC 5 cut(s) 200, 290, 513, 516, 996
GluI GCNGC 5 cut(s) 200, 290, 513, 516, 996
HaeIII GGCC 1 cut(s) 978
HapII CCGG 3 cut(s) 243, 585, 818
Hin1II CATG 7 cut(s) 211, 760, 770, 872, 899, 970, 1058
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 3 cut(s) 225, 284, 762
HpaII CCGG 3 cut(s) 243, 585, 818
HphI GGTGA 5 cut(s) 37, 37, 240, 323, 471
Hpy166II GTNNAC 4 cut(s) 447, 479, 901, 955
Hpy188I TCNGA 2 cut(s) 534, 1007
Hpy188III TCNNGA 3 cut(s) 55, 692, 1055
Hpy8I GTNNAC 4 cut(s) 447, 479, 901, 955
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 6 cut(s) 61, 362, 482, 592, 689, 815
HpyCH4III ACNGT 2 cut(s) 880, 1002
HpyCH4IV ACGT 3 cut(s) 75, 456, 957
HpyCH4V TGCA 5 cut(s) 155, 743, 841, 989, 1046
HpyF10VI GCNNNNNNNGC 2 cut(s) 521, 995
HpyF3I CTNAG 6 cut(s) 227, 316, 393, 591, 813, 913
HpySE526I ACGT 3 cut(s) 75, 456, 957
Hsp92II CATG 7 cut(s) 211, 760, 770, 872, 899, 970, 1058
Ksp22I TGATCA 1 cut(s) 1051
Kzo9I GATC 6 cut(s) 216, 604, 667, 792, 948, 1051
LguI GCTCTTC 1 cut(s) 91
Lsp1109I GCAGC 4 cut(s) 301, 524, 527, 1007
LweI GCATC 2 cut(s) 730, 828
MaeII ACGT 3 cut(s) 75, 456, 957
MaeIII GTNAC 4 cut(s) 76, 311, 826, 1019
MalI GATC 6 cut(s) 218, 606, 669, 794, 950, 1053
MboI GATC 6 cut(s) 216, 604, 667, 792, 948, 1051
MboII GAAGA 6 cut(s) 78, 178, 493, 625, 665, 974
MfeI CAATTG 1 cut(s) 334
MflI RGATCY 1 cut(s) 667
MluCI AATT 8 cut(s) 139, 334, 344, 426, 554, 620, 774, 832
MlyI GAGTC 3 cut(s) 219, 278, 771
MmeI TCCRAC 2 cut(s) 556, 717
MseI TTAA 3 cut(s) 303, 972, 1014
MspA1I CMGCKG 2 cut(s) 199, 518
MspI CCGG 3 cut(s) 243, 585, 818
MspR9I CCNGG 1 cut(s) 243
MunI CAATTG 1 cut(s) 334
MvnI CGCG 1 cut(s) 885
MwoI GCNNNNNNNGC 2 cut(s) 521, 995
NciI CCSGG 1 cut(s) 243
NcoI CCATGG 2 cut(s) 766, 868
NdeII GATC 6 cut(s) 216, 604, 667, 792, 948, 1051
NlaIII CATG 7 cut(s) 211, 760, 770, 872, 899, 970, 1058
NmeAIII GCCGAG 1 cut(s) 411
NmuCI GTSAC 3 cut(s) 76, 311, 1019
PaeR7I CTCGAG 1 cut(s) 980
PagI TCATGA 1 cut(s) 1054
PaqCI CACCTGC 1 cut(s) 299
PceI AGGCCT 1 cut(s) 978
PciSI GCTCTTC 1 cut(s) 91
Pfl23II CGTACG 1 cut(s) 453
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 5 cut(s) 201, 291, 514, 517, 997
PleI GAGTC 3 cut(s) 219, 278, 770
PpsI GAGTC 3 cut(s) 219, 278, 770
Ppu21I YACGTR 3 cut(s) 76, 457, 958
PshAI GACNNNNGTC 1 cut(s) 401
PspLI CGTACG 1 cut(s) 453
PspXI VCTCGAGB 1 cut(s) 980
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 5 cut(s) 97, 150, 455, 589, 686
RsaNI GTAC 5 cut(s) 96, 149, 454, 588, 685
SapI GCTCTTC 1 cut(s) 91
SaqAI TTAA 3 cut(s) 303, 972, 1014
SatI GCNGC 5 cut(s) 200, 290, 513, 516, 996
Sau3AI GATC 6 cut(s) 216, 604, 667, 792, 948, 1051
SchI GAGTC 3 cut(s) 219, 278, 771
ScrFI CCNGG 1 cut(s) 243
SfaNI GCATC 2 cut(s) 730, 828
Sfr274I CTCGAG 1 cut(s) 980
SlaI CTCGAG 1 cut(s) 980
SmlI CTYRAG 1 cut(s) 980
SmoI CTYRAG 1 cut(s) 980
SnaBI TACGTA 1 cut(s) 457
Sse9I AATT 8 cut(s) 139, 334, 344, 426, 554, 620, 774, 832
SseBI AGGCCT 1 cut(s) 978
SsiI CCGC 3 cut(s) 48, 199, 883
SspI AATATT 1 cut(s) 255
StuI AGGCCT 1 cut(s) 978
StyD4I CCNGG 1 cut(s) 241
StyI CCWWGG 3 cut(s) 483, 766, 868
TaaI ACNGT 2 cut(s) 880, 1002
TaiI ACGT 3 cut(s) 78, 459, 960
TaqI TCGA 2 cut(s) 630, 981
TasI AATT 8 cut(s) 139, 334, 344, 426, 554, 620, 774, 832
TauI GCSGC 1 cut(s) 202
Tru1I TTAA 3 cut(s) 303, 972, 1014
Tru9I TTAA 3 cut(s) 303, 972, 1014
TscAI CASTG 2 cut(s) 127, 1005
TseFI GTSAC 3 cut(s) 76, 311, 1019
TseI GCWGC 4 cut(s) 289, 512, 515, 995
Tsp45I GTSAC 3 cut(s) 76, 311, 1019
TspDTI ATGAA 4 cut(s) 224, 402, 493, 910
TspRI CASTG 2 cut(s) 127, 1005
Van91I CCANNNNNTGG 1 cut(s) 257
XapI RAATTY 3 cut(s) 139, 426, 832
XhoI CTCGAG 1 cut(s) 980
XmiI GTMKAC 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.