MD01G1046700.v1.1

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000431
Physical Location & Seq
Forward (+)
14778939 .. 14781459
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1046700.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGGCAATGGCAATGGCGAGTGGCGTAGAGGAAGTGAGGAACAAGCAGGTGGTACTCAAGAACTATGTCTCTGAAAAAGCCGAAGAGTCCGACATGCACGTGACAGTTAGTACCTTGAAGCTCAAGGTTCCACAGGACTCCAAGGCAGTTGTGGTCAAGAACCTTTACTTGTCGTGCGACGCTTATTTGAGGATTCGAATGGATAGAACTCAACCTCCTAATAGTGTCTTCACTTCACTCACTCCTGGCTCTCCATTAAATGGGTTTGGAGTGGCTAAAATTTTGGAGTCGGGGCACCCGGAGTTCAAGGAAGGTGACTTGGTTTGGGGGACGACTGGATGGGAAGATTACAGCATCATCACAGAACCAGAACAGCTCTTTAAAATCCACCACACTGATGTTCCCCTTTCCAACTATACTGGACTTCTTGGTATGCCGGGTATAACTGCCTTCGTCGGTTTTCATGAAATCTGTTCGCTGAAGAAAGGAGATTATGTCTTCATTTCAGCCGCAGCTGGTGCAGTTGGTCAGCTTGTTGGGCAGTTTGCAAAGCTAATGGGATGCTATGTTGTTGGAAGTGTTGGAAGCAAAGAGAAGGTCGATCTACTAAAGAACAAGCTTGGATTTGATGAGGCTTTCAATTATAGGGAAGAGCCTGACCTGGAGGCAGCTTTGAAAAGGTACTTCCCTGAAGGCATTGACATTTACTTTGAGAACGTTGGAGGAAAAATGCTGGATGCTGTGCTCCTTAACATGAGACGTCATGGCCGAATTGCGGTATGCGGATTGATCTCATATTACAGTCTCAAACAGCCGCAAGACTTTAGCAACCTCGTCTCTATCATCTACAATCGGATTCGCATCGAAGGCTTCGTCGTGTTTGATTATTTCCACCTCTACCCCAAGTTTCTGGACATGGTGATCCCCTACATCAGAGAAGGGAAAATAGTGTATCTGGAAGACATAGCTGAAGGCCTTGAGAATGCTCCTGCAGCTCTTGTGGGACTTTTTAGTGGCAGAAATGTTGGAAATCAAGTAGTTTTGGTTGCCTATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

353

Amino Acids

39.15

Weight (kDa)

5.62

Isoelectric Point (pI)

31.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 15 - 129 6.4e-20 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 174 - 306 7.9e-25 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 207 - 347 8e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26320 AT1G26320 AT3G03080 AT3G59845 AT3G59845 AT5G16960 AT5G16960 AT5G16970 AT5G16980 AT5G16980 AT5G16980 AT5G16990 AT5G17000 AT5G17000 AT5G37940 AT5G37960 AT5G37980 AT5G38000 AT5G38000 AT5G38000
fragaria_vesca FvH4_1g00340 FvH4_1g18790 FvH4_1g23120 FvH4_1g23120
malus_domestica MD00G1045700.v1.1 MD01G1046700.v1.1 MD02G1163900.v1.1 MD15G1145600.v1.1 MD16G1282000.v1.1
prunus_persica Prupe.1G543700_v2.0.a1 Prupe.4G262000_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.4G262300_v2.0.a1 Prupe.6G211500_v2.0.a1 Prupe.7G269100_v2.0.a1
pyrus_communis pycom02g00040 pycom02g13040 pycom11g16640 pycom12561g00320 pycom15g13080 pycom16g25160
rosa_chinensis RchiOBHm_Chr2g0084641 RchiOBHm_Chr2g0084711 RchiOBHm_Chr2g0085361 RchiOBHm_Chr2g0085371 RchiOBHm_Chr2g0109651 RchiOBHm_Chr2g0117841 RchiOBHm_Chr6g0275751
rosa_laevigata RLG00000013436 RLG00000015629 RLG00000015633 RLG00000015680 RLG00000015681 RLG00000017753 RLG00000018376
rosa_multiflora Rmu_sc0000332.1_g000060 Rmu_sc0002352.1_g000008 Rmu_sc0002352.1_g000012 Rmu_sc0010860.1_g000005 Rmu_sc0012119.1_g000008
rosa_roxburghii Rroxscaffold_2G00126190 Rroxscaffold_2G00134060 Rroxscaffold_2G00155420 Rroxscaffold_2G00155430 Rroxscaffold_2G00155880 Rroxscaffold_2G00155890
rosa_rugosa Rorug01G0455500 Rorug01G0455600 Rorug01G0459700 Rorug01G0459800 Rorug01G0459800 Rorug01G0459900 Rorug02G0161600 Rorug02G0214400
rosa_samantha Rh2BG008800 Rh2BG282100 Rh2CG003900 Rh2CG004600 Rh2CG009500 Rh2CG009600 Rh2CG216100 Rh2CG216200 Rh2CG269100 Rh2CG269200 Rh2DG003700 Rh2DG004100 Rh2DG009900 Rh2DG219300 Rh2DG278400
rosa_wichuraiana Rw2G000300 Rw2G000330 Rw2G000770 Rw2G000780 Rw2G021390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 37
AatII GACGTC 1 cut(s) 763
Acc36I ACCTGC 1 cut(s) 37
AccB1I GGYRCC 1 cut(s) 294
AccB7I CCANNNNNTGG 1 cut(s) 260
AciI CCGC 4 cut(s) 510, 776, 783, 815
AclI AACGTT 1 cut(s) 717
AclWI GGATC 1 cut(s) 916
AcoI YGGCCR 1 cut(s) 766
AcsI RAATTY 1 cut(s) 279
AcuI CTGAAG 3 cut(s) 500, 711, 990
AcvI CACGTG 1 cut(s) 100
AcyI GRCGYC 1 cut(s) 760
AfaI GTAC 3 cut(s) 54, 112, 683
AfiI CCNNNNNNNGG 2 cut(s) 260, 775
AgsI TTSAA 4 cut(s) 118, 307, 640, 676
AjnI CCWGG 2 cut(s) 244, 660
AloI GAACNNNNNNTCC 2 cut(s) 199, 231
AluBI AGCT 9 cut(s) 121, 376, 515, 532, 553, 619, 671, 968, 995
AluI AGCT 9 cut(s) 121, 376, 515, 532, 553, 619, 671, 968, 995
Alw21I GWGCWC 1 cut(s) 747
Alw26I GTCTC 4 cut(s) 73, 751, 809, 841
AlwI GGATC 1 cut(s) 916
AoxI GGCC 2 cut(s) 766, 973
ApeKI GCWGC 3 cut(s) 512, 668, 992
ApoI RAATTY 1 cut(s) 279
ArsI GACNNNNNNTTYG 6 cut(s) 582, 614, 692, 724, 858, 890
AsuC2I CCSGG 2 cut(s) 299, 438
AsuHPI GGTGA 2 cut(s) 326, 931
AsuII TTCGAA 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 297
BanI GGYRCC 1 cut(s) 294
BbrPI CACGTG 1 cut(s) 100
BbsI GAAGAC 3 cut(s) 220, 490, 966
Bbv12I GWGCWC 1 cut(s) 747
BbvI GCAGC 3 cut(s) 524, 680, 1004
BccI CCATC 1 cut(s) 333
BciT130I CCWGG 2 cut(s) 246, 662
BcnI CCSGG 2 cut(s) 299, 438
BcoDI GTCTC 4 cut(s) 73, 751, 809, 841
BfmI CTRYAG 1 cut(s) 990
BfuAI ACCTGC 1 cut(s) 37
BisI GCNGC 5 cut(s) 510, 513, 669, 815, 993
BlsI GCNGC 5 cut(s) 511, 514, 670, 816, 994
Bme1390I CCNGG 4 cut(s) 246, 299, 438, 662
BmiI GGNNCC 2 cut(s) 129, 296
BmrFI CCNGG 4 cut(s) 246, 299, 438, 662
BmsI GCATC 4 cut(s) 363, 551, 727, 870
BpiI GAAGAC 3 cut(s) 220, 490, 966
BpmI CTGGAG 1 cut(s) 683
Bpu14I TTCGAA 1 cut(s) 196
BpuEI CTTGAG 3 cut(s) 41, 107, 998
BpuMI CCSGG 2 cut(s) 299, 438
BsaAI YACGTR 1 cut(s) 100
BsaBI GATNNNNATC 1 cut(s) 860
BsaHI GRCGYC 1 cut(s) 760
BsaJI CCNNGG 1 cut(s) 141
BsaXI ACNNNNNCTCC 4 cut(s) 199, 229, 480, 510
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 775
Bse1I ACTGG 2 cut(s) 340, 424
Bse3DI GCAATG 2 cut(s) 12, 18
Bse8I GATNNNNATC 1 cut(s) 860
BseBI CCWGG 2 cut(s) 246, 662
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 3 cut(s) 344, 566, 742
BseJI GATNNNNATC 1 cut(s) 860
BseLI CCNNNNNNNGG 2 cut(s) 260, 775
BseMI GCAATG 2 cut(s) 12, 18
BseNI ACTGG 2 cut(s) 340, 424
BseSI GKGCMC 1 cut(s) 297
BseXI GCAGC 3 cut(s) 524, 680, 1004
BsgI GTGCAG 1 cut(s) 540
BshFI GGCC 2 cut(s) 768, 975
BshNI GGYRCC 1 cut(s) 294
BsiHKAI GWGCWC 1 cut(s) 747
BsiSI CCGG 2 cut(s) 299, 437
BslFI GGGAC 2 cut(s) 343, 1017
BslI CCNNNNNNNGG 2 cut(s) 260, 775
BsmAI GTCTC 4 cut(s) 73, 751, 809, 841
BsmBI CGTCTC 2 cut(s) 751, 841
BsmFI GGGAC 2 cut(s) 343, 1017
BsmI GAATGC 1 cut(s) 988
BsnI GGCC 2 cut(s) 768, 975
Bsp119I TTCGAA 1 cut(s) 196
Bsp1286I GDGCHC 2 cut(s) 297, 747
Bsp143I GATC 3 cut(s) 601, 789, 921
BspACI CCGC 4 cut(s) 510, 776, 783, 815
BspANI GGCC 2 cut(s) 768, 975
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 2 cut(s) 129, 296
BspMAI CTGCAG 1 cut(s) 994
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 1 cut(s) 916
BspQI GCTCTTC 1 cut(s) 645
BspT104I TTCGAA 1 cut(s) 196
BspT107I GGYRCC 1 cut(s) 294
BsrDI GCAATG 2 cut(s) 12, 18
BsrI ACTGG 2 cut(s) 340, 424
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 3 cut(s) 601, 789, 921
BssNI GRCGYC 1 cut(s) 760
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 246, 662
Bst4CI ACNGT 2 cut(s) 106, 803
Bst6I CTCTTC 2 cut(s) 78, 645
BstACI GRCGYC 1 cut(s) 760
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 100
BstBI TTCGAA 1 cut(s) 196
BstF5I GGATG 3 cut(s) 344, 566, 742
BstKTI GATC 3 cut(s) 604, 792, 924
BstMAI GTCTC 4 cut(s) 73, 751, 809, 841
BstMBI GATC 3 cut(s) 601, 789, 921
BstMWI GCNNNNNNNGC 4 cut(s) 518, 538, 867, 992
BstNI CCWGG 2 cut(s) 246, 662
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 4 cut(s) 244, 297, 436, 660
BstSFI CTRYAG 1 cut(s) 990
BstSLI GKGCMC 1 cut(s) 297
BstV1I GCAGC 3 cut(s) 524, 680, 1004
BstV2I GAAGAC 3 cut(s) 220, 490, 966
BstXI CCANNNNNNTGG 1 cut(s) 910
BsuRI GGCC 2 cut(s) 768, 975
BtsCI GGATG 3 cut(s) 344, 566, 742
BtsIMutI CAGTG 1 cut(s) 393
BveI ACCTGC 1 cut(s) 37
CciI TCATGA 1 cut(s) 463
CseI GACGC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 53, 111, 682
CviAII CATG 5 cut(s) 94, 464, 754, 764, 916
CviQI GTAC 3 cut(s) 53, 111, 682
DpnI GATC 3 cut(s) 603, 791, 923
DpnII GATC 3 cut(s) 601, 789, 921
DraI TTTAAA 1 cut(s) 382
EaeI YGGCCR 1 cut(s) 766
Eam1104I CTCTTC 2 cut(s) 78, 645
EarI CTCTTC 2 cut(s) 78, 645
Eco130I CCWWGG 1 cut(s) 141
Eco147I AGGCCT 1 cut(s) 975
Eco57I CTGAAG 3 cut(s) 500, 711, 990
Eco72I CACGTG 1 cut(s) 100
EcoRII CCWGG 2 cut(s) 244, 660
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
Esp3I CGTCTC 2 cut(s) 751, 841
FaeI CATG 5 cut(s) 97, 467, 757, 767, 919
FaqI GGGAC 2 cut(s) 343, 1017
FatI CATG 5 cut(s) 93, 463, 753, 763, 915
Fnu4HI GCNGC 5 cut(s) 510, 513, 669, 815, 993
FokI GGATG 3 cut(s) 351, 573, 749
Fsp4HI GCNGC 5 cut(s) 510, 513, 669, 815, 993
GluI GCNGC 5 cut(s) 510, 513, 669, 815, 993
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 2 cut(s) 768, 975
HapII CCGG 2 cut(s) 299, 437
HgaI GACGC 1 cut(s) 188
Hin1I GRCGYC 1 cut(s) 760
Hin1II CATG 5 cut(s) 97, 467, 757, 767, 919
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 5 cut(s) 86, 137, 193, 287, 856
HpaII CCGG 2 cut(s) 299, 437
HphI GGTGA 2 cut(s) 326, 931
Hpy188I TCNGA 4 cut(s) 73, 91, 855, 935
Hpy188III TCNNGA 5 cut(s) 58, 157, 464, 911, 956
Hpy99I CGWCG 3 cut(s) 182, 458, 878
HpyAV CCTTC 7 cut(s) 305, 460, 589, 686, 860, 932, 965
HpyCH4III ACNGT 2 cut(s) 106, 803
HpyCH4IV ACGT 3 cut(s) 99, 717, 760
HpyCH4V TGCA 4 cut(s) 97, 521, 548, 992
HpyF10VI GCNNNNNNNGC 4 cut(s) 518, 538, 867, 992
HpySE526I ACGT 3 cut(s) 99, 717, 760
Hsp92I GRCGYC 1 cut(s) 760
Hsp92II CATG 5 cut(s) 97, 467, 757, 767, 919
Kzo9I GATC 3 cut(s) 601, 789, 921
LguI GCTCTTC 1 cut(s) 645
LmnI GCTCC 2 cut(s) 750, 991
Lsp1109I GCAGC 3 cut(s) 524, 680, 1004
LweI GCATC 4 cut(s) 363, 551, 727, 870
MaeII ACGT 3 cut(s) 99, 717, 760
MaeIII GTNAC 2 cut(s) 100, 314
MalI GATC 3 cut(s) 603, 791, 923
MboI GATC 3 cut(s) 601, 789, 921
MboII GAAGA 7 cut(s) 95, 220, 356, 490, 493, 662, 971
MhlI GDGCHC 2 cut(s) 297, 747
MluCI AATT 3 cut(s) 279, 640, 771
MlyI GAGTC 3 cut(s) 95, 131, 296
MmeI TCCRAC 6 cut(s) 114, 435, 553, 562, 700, 1006
MnlI CCTC 9 cut(s) 22, 30, 183, 225, 625, 658, 716, 842, 905
MseI TTAA 3 cut(s) 257, 381, 750
MslI CAYNNNNRTG 2 cut(s) 98, 396
MspA1I CMGCKG 1 cut(s) 515
MspI CCGG 2 cut(s) 299, 437
MspR9I CCNGG 4 cut(s) 246, 299, 438, 662
Mva1269I GAATGC 1 cut(s) 988
MvaI CCWGG 2 cut(s) 246, 662
MwoI GCNNNNNNNGC 4 cut(s) 518, 538, 867, 992
NciI CCSGG 2 cut(s) 299, 438
NdeII GATC 3 cut(s) 601, 789, 921
NlaIII CATG 5 cut(s) 97, 467, 757, 767, 919
NlaIV GGNNCC 2 cut(s) 129, 296
NmuCI GTSAC 2 cut(s) 100, 314
NspI RCATGY 1 cut(s) 97
NspV TTCGAA 1 cut(s) 196
PagI TCATGA 1 cut(s) 463
PaqCI CACCTGC 1 cut(s) 37
PceI AGGCCT 1 cut(s) 975
PciSI GCTCTTC 1 cut(s) 645
PcsI WCGNNNNNNNCGW 2 cut(s) 766, 870
PctI GAATGC 1 cut(s) 988
PfeI GAWTC 2 cut(s) 193, 856
PflMI CCANNNNNTGG 1 cut(s) 260
PkrI GCNGC 5 cut(s) 511, 514, 670, 816, 994
PleI GAGTC 3 cut(s) 94, 131, 295
PmaCI CACGTG 1 cut(s) 100
PmlI CACGTG 1 cut(s) 100
PpsI GAGTC 3 cut(s) 94, 131, 295
Ppu21I YACGTR 1 cut(s) 100
Psp1406I AACGTT 1 cut(s) 717
Psp6I CCWGG 2 cut(s) 244, 660
PspCI CACGTG 1 cut(s) 100
PspGI CCWGG 2 cut(s) 244, 660
PspN4I GGNNCC 2 cut(s) 129, 296
PstI CTGCAG 1 cut(s) 994
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 3 cut(s) 54, 112, 683
RsaNI GTAC 3 cut(s) 53, 111, 682
RseI CAYNNNNRTG 2 cut(s) 98, 396
SapI GCTCTTC 1 cut(s) 645
SaqAI TTAA 3 cut(s) 257, 381, 750
SatI GCNGC 5 cut(s) 510, 513, 669, 815, 993
Sau3AI GATC 3 cut(s) 601, 789, 921
SchI GAGTC 3 cut(s) 95, 131, 296
ScrFI CCNGG 4 cut(s) 246, 299, 438, 662
SduI GDGCHC 2 cut(s) 297, 747
SfaNI GCATC 4 cut(s) 363, 551, 727, 870
SfcI CTRYAG 1 cut(s) 990
SfuI TTCGAA 1 cut(s) 196
SmiMI CAYNNNNRTG 2 cut(s) 98, 396
SmlI CTYRAG 3 cut(s) 56, 122, 977
SmoI CTYRAG 3 cut(s) 56, 122, 977
Sse9I AATT 3 cut(s) 279, 640, 771
SseBI AGGCCT 1 cut(s) 975
SsiI CCGC 4 cut(s) 510, 776, 783, 815
StuI AGGCCT 1 cut(s) 975
StyD4I CCNGG 4 cut(s) 244, 297, 436, 660
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 106, 803
TaiI ACGT 3 cut(s) 102, 720, 763
TaqI TCGA 3 cut(s) 196, 600, 864
TasI AATT 3 cut(s) 279, 640, 771
TauI GCSGC 2 cut(s) 512, 817
TfiI GAWTC 2 cut(s) 193, 856
Tru1I TTAA 3 cut(s) 257, 381, 750
Tru9I TTAA 3 cut(s) 257, 381, 750
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 2 cut(s) 100, 314
TseI GCWGC 3 cut(s) 512, 668, 992
Tsp45I GTSAC 2 cut(s) 100, 314
TspDTI ATGAA 3 cut(s) 452, 480, 490
TspRI CASTG 1 cut(s) 400
Van91I CCANNNNNTGG 1 cut(s) 260
XapI RAATTY 1 cut(s) 279
XceI RCATGY 1 cut(s) 97
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
ZraI GACGTC 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.