2-alkenal reductase (NADP( )-dependent)-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 317 |
| Acc36I | ACCTGC | 1 cut(s) | 317 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 275 |
| AccI | GTMKAC | 3 cut(s) | 222, 249, 877 |
| AciI | CCGC | 4 cut(s) | 193, 468, 744, 761 |
| AclWI | GGATC | 1 cut(s) | 176 |
| AcoI | YGGCCR | 2 cut(s) | 559, 787 |
| AcsI | RAATTY | 3 cut(s) | 444, 488, 926 |
| AcuI | CTGAAG | 1 cut(s) | 312 |
| AdeI | CACNNNGTG | 1 cut(s) | 865 |
| AfaI | GTAC | 4 cut(s) | 454, 607, 821, 869 |
| AfeI | AGCGCT | 1 cut(s) | 85 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 192, 275, 506 |
| AgsI | TTSAA | 6 cut(s) | 106, 138, 631, 697, 956, 992 |
| AjnI | CCWGG | 2 cut(s) | 214, 709 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 882, 914 |
| AluBI | AGCT | 3 cut(s) | 109, 536, 553 |
| AluI | AGCT | 3 cut(s) | 109, 536, 553 |
| AlwI | GGATC | 1 cut(s) | 176 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 536 |
| Aor51HI | AGCGCT | 1 cut(s) | 85 |
| AoxI | GGCC | 3 cut(s) | 559, 787, 994 |
| ApeKI | GCWGC | 3 cut(s) | 530, 533, 1013 |
| ApoI | RAATTY | 3 cut(s) | 444, 488, 926 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 811 |
| AspLEI | GCGC | 1 cut(s) | 86 |
| AsuC2I | CCSGG | 1 cut(s) | 261 |
| AsuHPI | GGTGA | 4 cut(s) | 37, 37, 341, 856 |
| BaeGI | GKGCMC | 1 cut(s) | 426 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 405, 438 |
| BalI | TGGCCA | 1 cut(s) | 561 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 152, 184, 589, 621 |
| BbsI | GAAGAC | 2 cut(s) | 757, 987 |
| BbvI | GCAGC | 3 cut(s) | 542, 545, 1025 |
| BceAI | ACGGC | 1 cut(s) | 134 |
| BciT130I | CCWGG | 2 cut(s) | 216, 711 |
| BclI | TGATCA | 3 cut(s) | 829, 904, 1069 |
| BcnI | CCSGG | 1 cut(s) | 261 |
| BfaI | CTAG | 1 cut(s) | 93 |
| BfoI | RGCGCY | 1 cut(s) | 87 |
| BfuAI | ACCTGC | 1 cut(s) | 317 |
| BisI | GCNGC | 5 cut(s) | 194, 531, 534, 745, 1014 |
| BlsI | GCNGC | 5 cut(s) | 195, 532, 535, 746, 1015 |
| Bme1390I | CCNGG | 3 cut(s) | 216, 261, 711 |
| BmiI | GGNNCC | 1 cut(s) | 144 |
| BmrFI | CCNGG | 3 cut(s) | 216, 261, 711 |
| BmsI | GCATC | 3 cut(s) | 748, 846, 928 |
| BpiI | GAAGAC | 2 cut(s) | 757, 987 |
| BpmI | CTGGAG | 2 cut(s) | 732, 993 |
| BpuEI | CTTGAG | 1 cut(s) | 1019 |
| BpuMI | CCSGG | 1 cut(s) | 261 |
| BsaAI | YACGTR | 1 cut(s) | 234 |
| BsaBI | GATNNNNATC | 1 cut(s) | 909 |
| BsaJI | CCNNGG | 3 cut(s) | 214, 784, 886 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 501, 531 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 192, 275, 506 |
| Bse1I | ACTGG | 1 cut(s) | 463 |
| Bse8I | GATNNNNATC | 1 cut(s) | 909 |
| BseBI | CCWGG | 2 cut(s) | 216, 711 |
| BseDI | CCNNGG | 3 cut(s) | 214, 784, 886 |
| BseGI | GGATG | 3 cut(s) | 691, 763, 919 |
| BseJI | GATNNNNATC | 1 cut(s) | 909 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 192, 275, 506 |
| BseNI | ACTGG | 1 cut(s) | 463 |
| BseSI | GKGCMC | 1 cut(s) | 426 |
| BseXI | GCAGC | 3 cut(s) | 542, 545, 1025 |
| BshFI | GGCC | 3 cut(s) | 561, 789, 996 |
| BsiSI | CCGG | 1 cut(s) | 261 |
| BslFI | GGGAC | 1 cut(s) | 358 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 192, 275, 506 |
| BsmFI | GGGAC | 1 cut(s) | 358 |
| BsmI | GAATGC | 1 cut(s) | 1009 |
| BsnI | GGCC | 3 cut(s) | 561, 789, 996 |
| Bsp1286I | GDGCHC | 1 cut(s) | 426 |
| Bsp1407I | TGTACA | 1 cut(s) | 867 |
| Bsp143I | GATC | 6 cut(s) | 181, 622, 829, 904, 966, 1069 |
| Bsp19I | CCATGG | 2 cut(s) | 784, 886 |
| BspACI | CCGC | 4 cut(s) | 193, 468, 744, 761 |
| BspANI | GGCC | 3 cut(s) | 561, 789, 996 |
| BspHI | TCATGA | 1 cut(s) | 1072 |
| BspLI | GGNNCC | 1 cut(s) | 144 |
| BspMI | ACCTGC | 1 cut(s) | 317 |
| BspPI | GGATC | 1 cut(s) | 176 |
| BspQI | GCTCTTC | 1 cut(s) | 626 |
| BsrGI | TGTACA | 1 cut(s) | 867 |
| BsrI | ACTGG | 1 cut(s) | 463 |
| BssECI | CCNNGG | 3 cut(s) | 214, 784, 886 |
| BssMI | GATC | 6 cut(s) | 181, 622, 829, 904, 966, 1069 |
| BssNAI | GTATAC | 1 cut(s) | 878 |
| BssT1I | CCWWGG | 2 cut(s) | 784, 886 |
| Bst1107I | GTATAC | 1 cut(s) | 878 |
| Bst2UI | CCWGG | 2 cut(s) | 216, 711 |
| Bst4CI | ACNGT | 2 cut(s) | 819, 898 |
| Bst6I | CTCTTC | 2 cut(s) | 626, 666 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 539 |
| BstAUI | TGTACA | 1 cut(s) | 867 |
| BstBAI | YACGTR | 1 cut(s) | 234 |
| BstC8I | GCNNGC | 1 cut(s) | 266 |
| BstDEI | CTNAG | 2 cut(s) | 609, 931 |
| BstDSI | CCRYGG | 2 cut(s) | 784, 886 |
| BstEII | GGTNACC | 1 cut(s) | 844 |
| BstF5I | GGATG | 3 cut(s) | 691, 763, 919 |
| BstH2I | RGCGCY | 1 cut(s) | 87 |
| BstHHI | GCGC | 1 cut(s) | 86 |
| BstKTI | GATC | 6 cut(s) | 184, 625, 832, 907, 969, 1072 |
| BstMBI | GATC | 6 cut(s) | 181, 622, 829, 904, 966, 1069 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 539, 1013 |
| BstNI | CCWGG | 2 cut(s) | 216, 711 |
| BstPI | GGTNACC | 1 cut(s) | 844 |
| BstSCI | CCNGG | 3 cut(s) | 214, 259, 709 |
| BstSLI | GKGCMC | 1 cut(s) | 426 |
| BstSNI | TACGTA | 1 cut(s) | 234 |
| BstV1I | GCAGC | 3 cut(s) | 542, 545, 1025 |
| BstV2I | GAAGAC | 2 cut(s) | 757, 987 |
| BstZ17I | GTATAC | 1 cut(s) | 878 |
| BsuRI | GGCC | 3 cut(s) | 561, 789, 996 |
| BtgI | CCRYGG | 2 cut(s) | 784, 886 |
| BtsCI | GGATG | 3 cut(s) | 691, 763, 919 |
| BtsIMutI | CAGTG | 1 cut(s) | 408 |
| BveI | ACCTGC | 1 cut(s) | 317 |
| Cac8I | GCNNGC | 1 cut(s) | 266 |
| CaiI | CAGNNNCTG | 1 cut(s) | 536 |
| CciI | TCATGA | 1 cut(s) | 1072 |
| CfoI | GCGC | 1 cut(s) | 86 |
| CseI | GACGC | 1 cut(s) | 662 |
| Csp6I | GTAC | 4 cut(s) | 453, 606, 820, 868 |
| CviAII | CATG | 4 cut(s) | 775, 785, 887, 1073 |
| CviQI | GTAC | 4 cut(s) | 453, 606, 820, 868 |
| DdeI | CTNAG | 2 cut(s) | 609, 931 |
| DpnI | GATC | 6 cut(s) | 183, 624, 831, 906, 968, 1071 |
| DpnII | GATC | 6 cut(s) | 181, 622, 829, 904, 966, 1069 |
| DraIII | CACNNNGTG | 1 cut(s) | 865 |
| EaeI | YGGCCR | 2 cut(s) | 559, 787 |
| Eam1104I | CTCTTC | 2 cut(s) | 626, 666 |
| EarI | CTCTTC | 2 cut(s) | 626, 666 |
| Eco105I | TACGTA | 1 cut(s) | 234 |
| Eco130I | CCWWGG | 2 cut(s) | 784, 886 |
| Eco147I | AGGCCT | 1 cut(s) | 996 |
| Eco47III | AGCGCT | 1 cut(s) | 85 |
| Eco57I | CTGAAG | 1 cut(s) | 312 |
| Eco91I | GGTNACC | 1 cut(s) | 844 |
| EcoO65I | GGTNACC | 1 cut(s) | 844 |
| EcoRI | GAATTC | 2 cut(s) | 444, 488 |
| EcoRII | CCWGG | 2 cut(s) | 214, 709 |
| EcoT14I | CCWWGG | 2 cut(s) | 784, 886 |
| ErhI | CCWWGG | 2 cut(s) | 784, 886 |
| FaeI | CATG | 4 cut(s) | 778, 788, 890, 1076 |
| FaqI | GGGAC | 1 cut(s) | 358 |
| FatI | CATG | 4 cut(s) | 774, 784, 886, 1072 |
| FbaI | TGATCA | 3 cut(s) | 829, 904, 1069 |
| FblI | GTMKAC | 3 cut(s) | 222, 249, 877 |
| Fnu4HI | GCNGC | 5 cut(s) | 194, 531, 534, 745, 1014 |
| FokI | GGATG | 3 cut(s) | 698, 770, 906 |
| Fsp4HI | GCNGC | 5 cut(s) | 194, 531, 534, 745, 1014 |
| FspBI | CTAG | 1 cut(s) | 93 |
| GlaI | GCGC | 1 cut(s) | 85 |
| GluI | GCNGC | 5 cut(s) | 194, 531, 534, 745, 1014 |
| GsuI | CTGGAG | 2 cut(s) | 732, 993 |
| HaeII | RGCGCY | 1 cut(s) | 87 |
| HaeIII | GGCC | 3 cut(s) | 561, 789, 996 |
| HapII | CCGG | 1 cut(s) | 261 |
| HgaI | GACGC | 1 cut(s) | 662 |
| HhaI | GCGC | 1 cut(s) | 86 |
| Hin1II | CATG | 4 cut(s) | 778, 788, 890, 1076 |
| Hin6I | GCGC | 1 cut(s) | 84 |
| HinP1I | GCGC | 1 cut(s) | 84 |
| HincII | GTYRAC | 1 cut(s) | 223 |
| HindII | GTYRAC | 1 cut(s) | 223 |
| HindIII | AAGCTT | 1 cut(s) | 107 |
| HinfI | GANTC | 5 cut(s) | 62, 219, 227, 246, 302 |
| HpaII | CCGG | 1 cut(s) | 261 |
| HphI | GGTGA | 4 cut(s) | 37, 37, 341, 856 |
| Hpy166II | GTNNAC | 4 cut(s) | 223, 250, 868, 878 |
| Hpy188I | TCNGA | 3 cut(s) | 57, 67, 501 |
| Hpy188III | TCNNGA | 3 cut(s) | 827, 902, 1073 |
| Hpy8I | GTNNAC | 4 cut(s) | 223, 250, 868, 878 |
| Hpy99I | CGWCG | 1 cut(s) | 227 |
| HpyAV | CCTTC | 2 cut(s) | 833, 986 |
| HpyCH4III | ACNGT | 2 cut(s) | 819, 898 |
| HpyCH4IV | ACGT | 3 cut(s) | 5, 233, 474 |
| HpyCH4V | TGCA | 3 cut(s) | 859, 919, 1064 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 539, 1013 |
| HpyF3I | CTNAG | 2 cut(s) | 609, 931 |
| HpySE526I | ACGT | 3 cut(s) | 5, 233, 474 |
| Hsp92II | CATG | 4 cut(s) | 778, 788, 890, 1076 |
| HspAI | GCGC | 1 cut(s) | 84 |
| Ksp22I | TGATCA | 3 cut(s) | 829, 904, 1069 |
| Kzo9I | GATC | 6 cut(s) | 181, 622, 829, 904, 966, 1069 |
| LguI | GCTCTTC | 1 cut(s) | 626 |
| LmnI | GCTCC | 2 cut(s) | 358, 1012 |
| Lsp1109I | GCAGC | 3 cut(s) | 542, 545, 1025 |
| LweI | GCATC | 3 cut(s) | 748, 846, 928 |
| MaeI | CTAG | 1 cut(s) | 93 |
| MaeII | ACGT | 3 cut(s) | 5, 233, 474 |
| MaeIII | GTNAC | 6 cut(s) | 43, 76, 329, 677, 844, 1037 |
| MalI | GATC | 6 cut(s) | 183, 624, 831, 906, 968, 1071 |
| MboI | GATC | 6 cut(s) | 181, 622, 829, 904, 966, 1069 |
| MboII | GAAGA | 8 cut(s) | 172, 218, 514, 643, 683, 757, 976, 992 |
| MhlI | GDGCHC | 1 cut(s) | 426 |
| MlsI | TGGCCA | 1 cut(s) | 561 |
| MluCI | AATT | 7 cut(s) | 352, 444, 488, 564, 792, 850, 926 |
| MluNI | TGGCCA | 1 cut(s) | 561 |
| MlyI | GAGTC | 3 cut(s) | 228, 255, 296 |
| MmeI | TCCRAC | 2 cut(s) | 279, 574 |
| MnlI | CCTC | 5 cut(s) | 27, 176, 401, 707, 779 |
| Mox20I | TGGCCA | 1 cut(s) | 561 |
| MroXI | GAANNNNTTC | 1 cut(s) | 811 |
| MscI | TGGCCA | 1 cut(s) | 561 |
| MseI | TTAA | 3 cut(s) | 321, 660, 1032 |
| Msp20I | TGGCCA | 1 cut(s) | 561 |
| MspA1I | CMGCKG | 2 cut(s) | 193, 536 |
| MspI | CCGG | 1 cut(s) | 261 |
| MspR9I | CCNGG | 3 cut(s) | 216, 261, 711 |
| Mva1269I | GAATGC | 1 cut(s) | 1009 |
| MvaI | CCWGG | 2 cut(s) | 216, 711 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 539, 1013 |
| NciI | CCSGG | 1 cut(s) | 261 |
| NcoI | CCATGG | 2 cut(s) | 784, 886 |
| NdeII | GATC | 6 cut(s) | 181, 622, 829, 904, 966, 1069 |
| NlaIII | CATG | 4 cut(s) | 778, 788, 890, 1076 |
| NlaIV | GGNNCC | 1 cut(s) | 144 |
| NmuCI | GTSAC | 6 cut(s) | 43, 76, 329, 677, 844, 1037 |
| PagI | TCATGA | 1 cut(s) | 1072 |
| PaqCI | CACCTGC | 1 cut(s) | 317 |
| PceI | AGGCCT | 1 cut(s) | 996 |
| PciSI | GCTCTTC | 1 cut(s) | 626 |
| PctI | GAATGC | 1 cut(s) | 1009 |
| PdmI | GAANNNNTTC | 1 cut(s) | 811 |
| PfeI | GAWTC | 2 cut(s) | 62, 227 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 275 |
| PfoI | TCCNGGA | 1 cut(s) | 709 |
| PkrI | GCNGC | 5 cut(s) | 195, 532, 535, 746, 1015 |
| PleI | GAGTC | 3 cut(s) | 227, 254, 296 |
| PpsI | GAGTC | 3 cut(s) | 227, 254, 296 |
| Ppu21I | YACGTR | 1 cut(s) | 234 |
| Psp6I | CCWGG | 2 cut(s) | 214, 709 |
| PspEI | GGTNACC | 1 cut(s) | 844 |
| PspGI | CCWGG | 2 cut(s) | 214, 709 |
| PspN4I | GGNNCC | 1 cut(s) | 144 |
| PstNI | CAGNNNCTG | 1 cut(s) | 536 |
| PvuII | CAGCTG | 1 cut(s) | 536 |
| RsaI | GTAC | 4 cut(s) | 454, 607, 821, 869 |
| RsaNI | GTAC | 4 cut(s) | 453, 606, 820, 868 |
| SalI | GTCGAC | 1 cut(s) | 221 |
| SapI | GCTCTTC | 1 cut(s) | 626 |
| SaqAI | TTAA | 3 cut(s) | 321, 660, 1032 |
| SatI | GCNGC | 5 cut(s) | 194, 531, 534, 745, 1014 |
| Sau3AI | GATC | 6 cut(s) | 181, 622, 829, 904, 966, 1069 |
| SchI | GAGTC | 3 cut(s) | 228, 255, 296 |
| ScrFI | CCNGG | 3 cut(s) | 216, 261, 711 |
| SduI | GDGCHC | 1 cut(s) | 426 |
| SfaNI | GCATC | 3 cut(s) | 748, 846, 928 |
| SmlI | CTYRAG | 1 cut(s) | 998 |
| SmoI | CTYRAG | 1 cut(s) | 998 |
| SnaBI | TACGTA | 1 cut(s) | 234 |
| Sse9I | AATT | 7 cut(s) | 352, 444, 488, 564, 792, 850, 926 |
| SseBI | AGGCCT | 1 cut(s) | 996 |
| SsiI | CCGC | 4 cut(s) | 193, 468, 744, 761 |
| SspMI | CTAG | 1 cut(s) | 93 |
| StuI | AGGCCT | 1 cut(s) | 996 |
| StyD4I | CCNGG | 3 cut(s) | 214, 259, 709 |
| StyI | CCWWGG | 2 cut(s) | 784, 886 |
| TaaI | ACNGT | 2 cut(s) | 819, 898 |
| TaiI | ACGT | 3 cut(s) | 8, 236, 477 |
| TaqI | TCGA | 1 cut(s) | 222 |
| TasI | AATT | 7 cut(s) | 352, 444, 488, 564, 792, 850, 926 |
| TatI | WGTACW | 2 cut(s) | 819, 867 |
| TauI | GCSGC | 2 cut(s) | 196, 747 |
| TfiI | GAWTC | 2 cut(s) | 62, 227 |
| Tru1I | TTAA | 3 cut(s) | 321, 660, 1032 |
| Tru9I | TTAA | 3 cut(s) | 321, 660, 1032 |
| TscAI | CASTG | 1 cut(s) | 415 |
| TseFI | GTSAC | 6 cut(s) | 43, 76, 329, 677, 844, 1037 |
| TseI | GCWGC | 3 cut(s) | 530, 533, 1013 |
| Tsp45I | GTSAC | 6 cut(s) | 43, 76, 329, 677, 844, 1037 |
| TspDTI | ATGAA | 2 cut(s) | 501, 928 |
| TspRI | CASTG | 1 cut(s) | 415 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 275 |
| XapI | RAATTY | 3 cut(s) | 444, 488, 926 |
| XmiI | GTMKAC | 3 cut(s) | 222, 249, 877 |
| XmnI | GAANNNNTTC | 1 cut(s) | 811 |
| XspI | CTAG | 1 cut(s) | 93 |