FvH4_1g10430

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000489
Physical Location & Seq
Reverse (-)
5696572 .. 5706555
9984 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10430.t1

Sequence Viewer

Length: 2016 bp
ATGGATCCTTCCAGTAAGTCGACTGCAACTCGAGCATTGGCGAATGTACTTGGTGATGAGGATCAGGCCGGTTTAGAAGCGTGGTCGAACAACCAGCCTCCGCCGCTTGAAGCTGCAACAACAACCAAAGAGACCAAGATATTGAAGGGCATGGATCTAAACATCTTCATGGCGGCAACACAAGGCAACATTGACGCCCTTAAGGAACACAAGGACGTTCTCCACCAAATTTTGACTCCCACCAACAACACTGTTCTTCACGTCTATATAGCATACGAAAGCAGCAGCGCAGCAGCGGTGACCAAATCAGTTTATCAGCTTCTCGAGTCGACCAACAACGTCAAGAAAGTGCTTGATTTGTGTCCACCACTGCTACTTCAGCTCAACAAGAGTCGTGAGACTGCGTTACACATTGCCGCGAGGTACGGGCGTGCTGATATTGTTGAAGTGCTCATACAAGAAGCCAAAACTCGCGATTTAAATGGTGACAACCTTGAGGAAGGGTTTGGAAGACAATGGCAGGTCCTCATTAGAGGAAAAAACTGTGACGAAGACACTGCCTTGCACGAGGCGGTGCGGTTTAACCACCTAGAAGTGGTGAAGTTACTGACCAAAGAAGACCCCGAGTTCGTGTACCCTGCTAACAAAGCGGGTGAGACACCACTTTACTTAGCTGCTGAGAGAGGACACTATGATTTGGTTTGTGAAATACTTGATAATTGCAGATATGCAGCTTATTACCAAGGTCCTAATTGTTCAACTGCTTTACACGGCGCTGTCATATCCAACGATGAAAAAATGACAAGGAAACTACTGGAGAAGCATAAGAGTATGACACAACAAGTAGATGAACAAGGATGGACTCCGCTTCACTTGGCTGCATTCACGGGTCACAGTACGATCGCGAGATTGTTACTAGAATGTGATACATCTGCTGCCTACATTCGTGACAAAAATAAGAAGCGTACGCCTCTTCATCTTGCAGCCTCCCAAGGCCGTGTGGACGTGATGAAAGAGATTGTTTTTCGATGCCCTGATTGTTGCGAGCTGGTGGACTACAAACGTCAAAATGTTCTTCACTATGCTATTCAGAAACACCAACTTCAAGTAGAAAGATTTGTTCGAAAAGATCCGTGGCTTAGTCACGTACTTTTGAATGGGAAGGATGTTGGCAGAAACACGCCCCTCCATGACATTGCTTCTTCTCCTTACAAGGAAGCCACTGATTTCATAAGTGATGACAGAGTTGATAAGATGGCATTCAACAAGGAAAACTTAAACGCTATCAACATCATCGAGAAGAATAATAGGTCCTCACCATGGAAGAGAATTCTCAGAACGGAAATGGAAGAGATAGGTGCTTATCGTGGTTATCGTATTCCAAATCCTGACGAAGATGGGGGGAGGAAACTAGTCCATGTAAACAAAAATGACAATGACCTTAGAGACAATGTATTAAACAAGAAAGACCTCAAAGACAAGGAAATAAAAGAGTCTCATTTGGTAGTGTCTGCACTGATATCAACAGTAACCTTTGCTGCCGGCTTCACCGTGCCGGGTGGTTATGTGAGTGAAAAAGGACCAAACCAGGGTCTTGCGGTTCTATCAAGAAATGCAGCTTTCAAGGCATTTGTGATAACAAACACATTAGCCATGAGTCTGTCCAGTTGTGCAATCATGGTACGTTTTTTCTTATCGCTGAAAGGGCAACATGGATCAAAGTCGTTGTCTATGGCGATAAGCTGCACCTTTTACGCGCTGATTGCGATGGTGGCTGCGTTCCTCACCGGTGTATATGCAGTATTAGGTGGTCATGTTTCATTAGGACTTGCCGTCGCCGCTTGTGTGCTAGGCACTGTCTTCTTCTCGGTTTTAGGTTTTGTCTCTATTTCTCCCGCTTTCATTACTTTCCGCAAACGTGCACATCGCTTCTTTGTCTGGATTGTTTATTTGTCTGGATTGTTAAACAGCTCGGATTGCGGTATTATTTTTTGTACTGATCGAACATATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

672

Amino Acids

74.69

Weight (kDa)

8.08

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 56 - 154 3.5e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 120 - 162 1e-05 Ankyrin repeats (many copies)
Ank_2 PF12796 177 - 240 2.3e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 188 - 234 6.9e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 252 - 309 5.4e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 256 - 306 6.6e-08 Ankyrin repeats (many copies)
PGG PF13962 494 - 601 9.4e-28 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 511
AccI GTMKAC 2 cut(s) 20, 329
AccII CGCG 4 cut(s) 419, 474, 905, 1757
AclWI GGATC 5 cut(s) 12, 69, 162, 1124, 1723
AcsI RAATTY 2 cut(s) 228, 1329
AcuI CTGAAG 1 cut(s) 362
AcyI GRCGYC 1 cut(s) 195
AfaI GTAC 8 cut(s) 48, 425, 635, 898, 967, 1149, 1683, 1996
AfiI CCNNNNNNNGG 3 cut(s) 595, 1320, 1589
AflII CTTAAG 1 cut(s) 200
AgeI ACCGGT 1 cut(s) 1787
AgsI TTSAA 8 cut(s) 110, 145, 446, 759, 1106, 1156, 1264, 1624
AhdI GACNNNNNGTC 1 cut(s) 1832
AhlI ACTAGT 1 cut(s) 1411
AjiI CACGTC 2 cut(s) 262, 1006
AjnI CCWGG 1 cut(s) 1587
AluBI AGCT 9 cut(s) 113, 319, 382, 674, 734, 1048, 1619, 1743, 1971
AluI AGCT 9 cut(s) 113, 319, 382, 674, 734, 1048, 1619, 1743, 1971
Alw21I GWGCWC 2 cut(s) 453, 1924
Alw26I GTCTC 6 cut(s) 125, 392, 650, 1440, 1500, 1888
Alw44I GTGCAC 1 cut(s) 1920
AlwI GGATC 5 cut(s) 12, 69, 162, 1124, 1723
Ama87I CYCGRG 3 cut(s) 30, 323, 623
AoxI GGCC 2 cut(s) 66, 994
ApaLI GTGCAC 1 cut(s) 1920
ApoI RAATTY 2 cut(s) 228, 1329
ArsI GACNNNNNNTTYG 4 cut(s) 611, 643, 1712, 1744
AsiGI ACCGGT 1 cut(s) 1787
AspLEI GCGC 3 cut(s) 290, 776, 1759
AspS9I GGNCC 4 cut(s) 523, 746, 1311, 1580
AsuC2I CCSGG 1 cut(s) 1557
AsuHPI GGTGA 8 cut(s) 65, 310, 497, 610, 665, 1308, 1540, 1777
AsuII TTCGAA 1 cut(s) 1123
AvaI CYCGRG 3 cut(s) 30, 323, 623
AvaII GGWCC 4 cut(s) 523, 746, 1311, 1580
BaeGI GKGCMC 1 cut(s) 1924
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BamHI GGATCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 438, 470
BauI CACGAG 1 cut(s) 566
BbsI GAAGAC 4 cut(s) 517, 558, 624, 1852
Bbv12I GWGCWC 2 cut(s) 453, 1924
BccI CCATC 4 cut(s) 852, 1249, 1391, 1762
BceAI ACGGC 3 cut(s) 787, 981, 1817
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 1 cut(s) 1589
BcnI CCSGG 1 cut(s) 1557
BcoDI GTCTC 6 cut(s) 125, 392, 650, 1440, 1500, 1888
BcuI ACTAGT 1 cut(s) 1411
BfaI CTAG 4 cut(s) 590, 917, 1412, 1850
BfoI RGCGCY 1 cut(s) 777
BfrI CTTAAG 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 511
Bme1390I CCNGG 2 cut(s) 1557, 1589
Bme18I GGWCC 4 cut(s) 523, 746, 1311, 1580
BmeRI GACNNNNNGTC 1 cut(s) 1832
BmeT110I CYCGRG 3 cut(s) 30, 323, 623
BmgBI CACGTC 2 cut(s) 262, 1006
BmgT120I GGNCC 4 cut(s) 523, 746, 1311, 1580
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 1557, 1589
BmsI GCATC 1 cut(s) 1019
BpiI GAAGAC 4 cut(s) 517, 558, 624, 1852
BpmI CTGGAG 1 cut(s) 836
Bpu14I TTCGAA 1 cut(s) 1123
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 1557
BsaAI YACGTR 1 cut(s) 1147
BsaBI GATNNNNATC 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 195
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 5 cut(s) 742, 991, 1133, 1319, 1588
BsaWI WCCGGW 1 cut(s) 1787
Bsc4I CCNNNNNNNGG 3 cut(s) 595, 1320, 1589
Bse118I RCCGGY 3 cut(s) 68, 1541, 1787
Bse1I ACTGG 3 cut(s) 12, 819, 1665
Bse3DI GCAATG 2 cut(s) 411, 1194
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 1 cut(s) 1589
BseDI CCNNGG 5 cut(s) 742, 991, 1133, 1319, 1588
BseGI GGATG 2 cut(s) 863, 1171
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 3 cut(s) 595, 1320, 1589
BseMI GCAATG 2 cut(s) 411, 1194
BseMII CTCAG 2 cut(s) 669, 1348
BseNI ACTGG 3 cut(s) 12, 819, 1665
BseSI GKGCMC 1 cut(s) 1924
BsgI GTGCAG 2 cut(s) 1497, 1729
Bsh1236I CGCG 4 cut(s) 419, 474, 905, 1757
Bsh1285I CGRYCG 1 cut(s) 903
BshFI GGCC 2 cut(s) 68, 996
BshTI ACCGGT 1 cut(s) 1787
BsiEI CGRYCG 1 cut(s) 903
BsiHKAI GWGCWC 2 cut(s) 453, 1924
BsiHKCI CYCGRG 3 cut(s) 30, 323, 623
BsiSI CCGG 4 cut(s) 69, 1542, 1556, 1788
BsiWI CGTACG 1 cut(s) 965
BslI CCNNNNNNNGG 3 cut(s) 595, 1320, 1589
BsmAI GTCTC 6 cut(s) 125, 392, 650, 1440, 1500, 1888
BsmI GAATGC 2 cut(s) 881, 1259
BsnI GGCC 2 cut(s) 68, 996
Bso31I GGTCTC 1 cut(s) 125
BsoBI CYCGRG 3 cut(s) 30, 323, 623
Bsp119I TTCGAA 1 cut(s) 1123
Bsp1286I GDGCHC 2 cut(s) 453, 1924
Bsp143I GATC 7 cut(s) 4, 61, 154, 900, 1129, 1715, 1999
Bsp19I CCATGG 1 cut(s) 1319
Bsp68I TCGCGA 2 cut(s) 474, 905
BspANI GGCC 2 cut(s) 68, 996
BspCNI CTCAG 2 cut(s) 670, 1347
BspFNI CGCG 4 cut(s) 419, 474, 905, 1757
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 5 cut(s) 12, 69, 162, 1124, 1723
BspT104I TTCGAA 1 cut(s) 1123
BspTI CTTAAG 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 411, 1194
BsrFI RCCGGY 3 cut(s) 68, 1541, 1787
BsrI ACTGG 3 cut(s) 12, 819, 1665
BssAI RCCGGY 3 cut(s) 68, 1541, 1787
BssECI CCNNGG 5 cut(s) 742, 991, 1133, 1319, 1588
BssMI GATC 7 cut(s) 4, 61, 154, 900, 1129, 1715, 1999
BssNI GRCGYC 1 cut(s) 195
BssSI CACGAG 1 cut(s) 566
BssT1I CCWWGG 3 cut(s) 742, 991, 1319
Bst2BI CACGAG 1 cut(s) 566
Bst2UI CCWGG 1 cut(s) 1589
Bst4CI ACNGT 6 cut(s) 253, 545, 896, 1528, 1552, 1858
Bst6I CTCTTC 3 cut(s) 978, 1319, 1344
BstACI GRCGYC 1 cut(s) 195
BstAFI CTTAAG 1 cut(s) 200
BstBAI YACGTR 1 cut(s) 1147
BstBI TTCGAA 1 cut(s) 1123
BstC8I GCNNGC 3 cut(s) 432, 1046, 1543
BstDEI CTNAG 5 cut(s) 670, 678, 1139, 1334, 1442
BstDSI CCRYGG 2 cut(s) 1133, 1319
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 2 cut(s) 863, 1171
BstFNI CGCG 4 cut(s) 419, 474, 905, 1757
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 3 cut(s) 290, 776, 1759
BstKTI GATC 7 cut(s) 7, 64, 157, 903, 1132, 1718, 2002
BstMAI GTCTC 6 cut(s) 125, 392, 650, 1440, 1500, 1888
BstMBI GATC 7 cut(s) 4, 61, 154, 900, 1129, 1715, 1999
BstMCI CGRYCG 1 cut(s) 903
BstNI CCWGG 1 cut(s) 1589
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 2 cut(s) 1555, 1587
BstSLI GKGCMC 1 cut(s) 1924
BstUI CGCG 4 cut(s) 419, 474, 905, 1757
BstV2I GAAGAC 4 cut(s) 517, 558, 624, 1852
BstX2I RGATCY 3 cut(s) 4, 154, 1129
BstYI RGATCY 3 cut(s) 4, 154, 1129
BsuRI GGCC 2 cut(s) 68, 996
BtgI CCRYGG 2 cut(s) 1133, 1319
BtgZI GCGATG 2 cut(s) 1781, 1910
BtrI CACGTC 2 cut(s) 262, 1006
BtsCI GGATG 2 cut(s) 863, 1171
BtsI GCAGTG 2 cut(s) 368, 555
BtsIMutI CAGTG 6 cut(s) 249, 368, 555, 1221, 1514, 1854
BtuMI TCGCGA 2 cut(s) 474, 905
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 3 cut(s) 432, 1046, 1543
CfoI GCGC 3 cut(s) 290, 776, 1759
Cfr10I RCCGGY 3 cut(s) 68, 1541, 1787
Cfr13I GGNCC 4 cut(s) 523, 746, 1311, 1580
CseI GACGC 1 cut(s) 203
Csp6I GTAC 8 cut(s) 47, 424, 634, 897, 966, 1148, 1682, 1995
CspAI ACCGGT 1 cut(s) 1787
CviAII CATG 9 cut(s) 151, 169, 1190, 1320, 1418, 1654, 1678, 1712, 1814
CviQI GTAC 8 cut(s) 47, 424, 634, 897, 966, 1148, 1682, 1995
DdeI CTNAG 5 cut(s) 670, 678, 1139, 1334, 1442
DpnI GATC 7 cut(s) 6, 63, 156, 902, 1131, 1717, 2001
DpnII GATC 7 cut(s) 4, 61, 154, 900, 1129, 1715, 1999
DraI TTTAAA 1 cut(s) 480
DriI GACNNNNNGTC 1 cut(s) 1832
Eam1104I CTCTTC 3 cut(s) 978, 1319, 1344
Eam1105I GACNNNNNGTC 1 cut(s) 1832
EarI CTCTTC 3 cut(s) 978, 1319, 1344
EciI GGCGGA 1 cut(s) 90
Eco130I CCWWGG 3 cut(s) 742, 991, 1319
Eco31I GGTCTC 1 cut(s) 125
Eco32I GATATC 1 cut(s) 1521
Eco47I GGWCC 4 cut(s) 523, 746, 1311, 1580
Eco57I CTGAAG 1 cut(s) 362
Eco88I CYCGRG 3 cut(s) 30, 323, 623
Eco91I GGTNACC 1 cut(s) 298
EcoO109I RGGNCCY 3 cut(s) 523, 746, 1311
EcoO65I GGTNACC 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 1329
EcoRII CCWGG 1 cut(s) 1587
EcoRV GATATC 1 cut(s) 1521
EcoT14I CCWWGG 3 cut(s) 742, 991, 1319
ErhI CCWWGG 3 cut(s) 742, 991, 1319
FaeI CATG 9 cut(s) 154, 172, 1193, 1323, 1421, 1657, 1681, 1715, 1817
FalI AAGNNNNNCTT 2 cut(s) 1259, 1291
FatI CATG 9 cut(s) 150, 168, 1189, 1319, 1417, 1653, 1677, 1711, 1813
FauI CCCGC 2 cut(s) 643, 1903
FblI GTMKAC 2 cut(s) 20, 329
FokI GGATG 2 cut(s) 870, 1178
FspBI CTAG 4 cut(s) 590, 917, 1412, 1850
GlaI GCGC 3 cut(s) 289, 775, 1758
GsuI CTGGAG 1 cut(s) 836
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 2 cut(s) 68, 996
HapII CCGG 4 cut(s) 69, 1542, 1556, 1788
HgaI GACGC 1 cut(s) 203
HhaI GCGC 3 cut(s) 290, 776, 1759
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 9 cut(s) 154, 172, 1193, 1323, 1421, 1657, 1681, 1715, 1817
Hin6I GCGC 3 cut(s) 288, 774, 1757
HinP1I GCGC 3 cut(s) 288, 774, 1757
HincII GTYRAC 2 cut(s) 21, 330
HindII GTYRAC 2 cut(s) 21, 330
HinfI GANTC 6 cut(s) 235, 326, 391, 862, 1493, 1657
HpaII CCGG 4 cut(s) 69, 1542, 1556, 1788
HphI GGTGA 8 cut(s) 65, 310, 497, 610, 665, 1308, 1540, 1777
Hpy166II GTNNAC 8 cut(s) 21, 330, 365, 634, 1003, 1054, 1423, 1922
Hpy188I TCNGA 3 cut(s) 1092, 1337, 1975
Hpy8I GTNNAC 8 cut(s) 21, 330, 365, 634, 1003, 1054, 1423, 1922
Hpy99I CGWCG 1 cut(s) 1838
HpyAV CCTTC 4 cut(s) 18, 139, 494, 1156
HpyCH4III ACNGT 6 cut(s) 253, 545, 896, 1528, 1552, 1858
HpyCH4IV ACGT 8 cut(s) 216, 261, 339, 1005, 1063, 1146, 1684, 1918
HpyF3I CTNAG 5 cut(s) 670, 678, 1139, 1334, 1442
HpySE526I ACGT 8 cut(s) 216, 261, 339, 1005, 1063, 1146, 1684, 1918
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 9 cut(s) 154, 172, 1193, 1323, 1421, 1657, 1681, 1715, 1817
HspAI GCGC 3 cut(s) 288, 774, 1757
KroI GCCGGC 1 cut(s) 1541
KroNI GCCGGC 1 cut(s) 1543
Kzo9I GATC 7 cut(s) 4, 61, 154, 900, 1129, 1715, 1999
LweI GCATC 1 cut(s) 1019
MaeI CTAG 4 cut(s) 590, 917, 1412, 1850
MaeII ACGT 8 cut(s) 216, 261, 339, 1005, 1063, 1146, 1684, 1918
MalI GATC 7 cut(s) 6, 63, 156, 902, 1131, 1717, 2001
MboI GATC 7 cut(s) 4, 61, 154, 900, 1129, 1715, 1999
MflI RGATCY 3 cut(s) 4, 154, 1129
MhlI GDGCHC 2 cut(s) 453, 1924
MluCI AATT 4 cut(s) 228, 718, 751, 1329
MlyI GAGTC 6 cut(s) 229, 335, 400, 856, 1502, 1666
MmeI TCCRAC 1 cut(s) 810
MroNI GCCGGC 1 cut(s) 1541
MseI TTAA 6 cut(s) 201, 479, 582, 1277, 1457, 1964
MslI CAYNNNNRTG 1 cut(s) 167
MspA1I CMGCKG 1 cut(s) 296
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 4 cut(s) 69, 1542, 1556, 1788
MspR9I CCNGG 2 cut(s) 1557, 1589
Mva1269I GAATGC 2 cut(s) 881, 1259
MvaI CCWGG 1 cut(s) 1589
MvnI CGCG 4 cut(s) 419, 474, 905, 1757
NaeI GCCGGC 1 cut(s) 1543
NciI CCSGG 1 cut(s) 1557
NcoI CCATGG 1 cut(s) 1319
NdeII GATC 7 cut(s) 4, 61, 154, 900, 1129, 1715, 1999
NgoMIV GCCGGC 1 cut(s) 1541
NlaIII CATG 9 cut(s) 154, 172, 1193, 1323, 1421, 1657, 1681, 1715, 1817
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 6 cut(s) 298, 485, 545, 890, 947, 1142
NruI TCGCGA 2 cut(s) 474, 905
NspV TTCGAA 1 cut(s) 1123
PaeR7I CTCGAG 2 cut(s) 30, 323
PcsI WCGNNNNNNNCGW 1 cut(s) 1372
PctI GAATGC 2 cut(s) 881, 1259
PdiI GCCGGC 1 cut(s) 1543
Pfl23II CGTACG 1 cut(s) 965
PinAI ACCGGT 1 cut(s) 1787
Ple19I CGATCG 1 cut(s) 903
PleI GAGTC 6 cut(s) 229, 334, 399, 856, 1501, 1665
PpsI GAGTC 6 cut(s) 229, 334, 399, 856, 1501, 1665
Ppu21I YACGTR 1 cut(s) 1147
PpuMI RGGWCCY 3 cut(s) 523, 746, 1311
Psp5II RGGWCCY 3 cut(s) 523, 746, 1311
Psp6I CCWGG 1 cut(s) 1587
PspEI GGTNACC 1 cut(s) 298
PspGI CCWGG 1 cut(s) 1587
PspLI CGTACG 1 cut(s) 965
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 4 cut(s) 523, 746, 1311, 1580
PspPPI RGGWCCY 3 cut(s) 523, 746, 1311
PspXI VCTCGAGB 1 cut(s) 30
PsuI RGATCY 3 cut(s) 4, 154, 1129
PvuI CGATCG 1 cut(s) 903
RruI TCGCGA 2 cut(s) 474, 905
RsaI GTAC 8 cut(s) 48, 425, 635, 898, 967, 1149, 1683, 1996
RsaNI GTAC 8 cut(s) 47, 424, 634, 897, 966, 1148, 1682, 1995
RseI CAYNNNNRTG 1 cut(s) 167
SalI GTCGAC 2 cut(s) 19, 328
SaqAI TTAA 6 cut(s) 201, 479, 582, 1277, 1457, 1964
Sau3AI GATC 7 cut(s) 4, 61, 154, 900, 1129, 1715, 1999
Sau96I GGNCC 4 cut(s) 523, 746, 1311, 1580
SchI GAGTC 6 cut(s) 229, 335, 400, 856, 1502, 1666
ScrFI CCNGG 2 cut(s) 1557, 1589
SduI GDGCHC 2 cut(s) 453, 1924
SfaNI GCATC 1 cut(s) 1019
Sfr274I CTCGAG 2 cut(s) 30, 323
SfuI TTCGAA 1 cut(s) 1123
SgrAI CRCCGGYG 1 cut(s) 1787
SinI GGWCC 4 cut(s) 523, 746, 1311, 1580
SlaI CTCGAG 2 cut(s) 30, 323
SmiI ATTTAAAT 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 4 cut(s) 30, 200, 323, 494
SmoI CTYRAG 4 cut(s) 30, 200, 323, 494
SpeI ACTAGT 1 cut(s) 1411
Sse9I AATT 4 cut(s) 228, 718, 751, 1329
SspMI CTAG 4 cut(s) 590, 917, 1412, 1850
StyD4I CCNGG 2 cut(s) 1555, 1587
StyI CCWWGG 3 cut(s) 742, 991, 1319
SwaI ATTTAAAT 1 cut(s) 480
TaaI ACNGT 6 cut(s) 253, 545, 896, 1528, 1552, 1858
TaiI ACGT 8 cut(s) 219, 264, 342, 1008, 1066, 1149, 1687, 1921
TaqI TCGA 9 cut(s) 20, 31, 86, 324, 329, 1027, 1123, 1296, 2002
TasI AATT 4 cut(s) 228, 718, 751, 1329
TatI WGTACW 2 cut(s) 46, 1994
TauI GCSGC 4 cut(s) 106, 176, 419, 1841
Tru1I TTAA 6 cut(s) 201, 479, 582, 1277, 1457, 1964
Tru9I TTAA 6 cut(s) 201, 479, 582, 1277, 1457, 1964
TscAI CASTG 6 cut(s) 256, 375, 562, 1228, 1521, 1861
TseFI GTSAC 6 cut(s) 298, 485, 545, 890, 947, 1142
Tsp45I GTSAC 6 cut(s) 298, 485, 545, 890, 947, 1142
TspDTI ATGAA 8 cut(s) 157, 807, 864, 965, 1025, 1219, 1809, 1891
TspGWI ACGGA 2 cut(s) 1122, 1355
TspRI CASTG 6 cut(s) 256, 375, 562, 1228, 1521, 1861
Vha464I CTTAAG 1 cut(s) 200
VneI GTGCAC 1 cut(s) 1920
VpaK11BI GGWCC 4 cut(s) 523, 746, 1311, 1580
XapI RAATTY 2 cut(s) 228, 1329
XhoI CTCGAG 2 cut(s) 30, 323
XmiI GTMKAC 2 cut(s) 20, 329
XspI CTAG 4 cut(s) 590, 917, 1412, 1850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.