Rh2AG299500

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000489
Physical Location & Seq
Reverse (-)
37390075 .. 37392854
2780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG299500.1

Sequence Viewer

Length: 1824 bp
ATGGATCTTAATGTATACAAAGCCGCCAAAGAAGGCAATGTTGATTTCCTTGGACAACACAAAGAGCAACTTCACGAAATATTGACGCCAACTAAGAGCACAGTCCTCCATATTTACGTAGCATGCGCAATGCTGTCAACCAATCCCGAACAGGCCGCCAACATAGTCGAGAAGATCGCTGAAATGTGTCCACCACTACTGTGGCAGTCCAGCCAGAATGGTGACACTGCGCTGCACGTTGCGGCCAGGAACGGATTTACTACTATAGTCAAACGTCTTATAGAGCTGGCTCCCAAATTTAGGGCTCCTCATGGAGACCTTGAAAATGGGAGGTCCATCCAAGAAGAAGAAGAAGCCTGTGCGCCGAAGTTCATCATGATCAGGGCAATTAACAACGAGGGAGACACACCCTTGCACGAGGCAGTGAGGTTTAATCACCTTGGTGTGGTGAAAATGCTGACTGCAGAAGACCCTGAGTTTTACTACTCTGGCAATGCTACCGAAGAGACTTGCATAAATCCATCTTGTCAAGGTCCCAATGGAAGAACGGCTTTGCATGCTGCCGTCATATGGAATGATCAAGAAATAACGAGAAAAATACTTGAAAAGAAATTGGAGCTGACGAAAGCAGTAGATGAAGAGGGATGTACTCCACTTCACCTTGCAGCATCATTGGGTTATGCTTCAATTGTGAAACAGCTTCTCAAGTGTGATAGATCTAGTGCGTATGTAAGGGAGAAATATCAGCAGAGGACAGCTCTCCACTTTGCAGCATCCGGAGGCCATGAAGATGTGATGAAACAGCTTATTACTGAATGCCCCGATTGTTGTGAGTTGGTCGATCACAAACTTCAGAATGTCCTACATTGTGCTATTGACGGCTTCTGCTTTCACTCAGCACATAGTCTTGTTTTAAAAGATCCTTGGCTCAGTGAAGTCCTCTTAAACAGAAAGGATGAACATGGAAACACACCGCTCCACCAGCTTGCTGCTACTTCCCCTTTCCGCTTCATGAGGTTTTGTAATTCAGTACTAACAGATGCTAGAATTGACAAGATGTCATTCAACAAAGAAAATCAAAACGCATTGGACATCTTTCGGTGTGAAGAAATAAAAAATAACTCTCAAGTTCCATTAAAACAAAAGAAAAGGAAATTTACTGCCAATTTGAAACGGAATGGTGTCATTCCAGGTTGTCGAATTGGAAGCAAAAAGATTGTTGATGAGATAGCTAAGAACAATGAAGCTCAAGAAGGAGACTGTAAATTGGCAGATTCTGTTCTTATAGTGGCTGCACTGATAGCAACTGTAAGTTTTGCAGGTTTTTTATCCATACCTTACCCCAGTGAAAAAGAACCTGATCAGGGTCTAGCAATTTCTACCAGAAGGACAGCTTTCCAGACATTCGTTATTACGAATACCATCGCTATGACTTTGTCCAGTTGTAGCGTCTTAGTGCACCTCTACTTGGGTCTTTTTGGAAAGCGTAAAATTATATCTGAAATATCCCAATCGATGGCTGGGTTTTCTATGTTGGCTTTATTTGCCCTGGTGATTGCCTTCATTGCTGGCACATATGCCGTATTGGGAAATTCTTCAGCAATGGCGGTTTCTGGTATTAGTTGCTTTCTGTTCTTCGGGCTATTTGAGACCATGGTTTATCAAGGAAGAATCGAAGGACCAAACTTCGTGGCCTTGGTCTTTTTACATGAGATCATTTTATGGCTGATAGAGAGCATGGAAAGCGGAATAATTAGACTCTACAAATATTTCCGAGACCTTGCTCAGTGCTCACCCGCTGGCCTCAAGTCTAGTTTTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

607

Amino Acids

67.39

Weight (kDa)

6.56

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 69 - 155 1.1e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 173 - 237 9.9e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 185 - 233 7e-06 Ankyrin repeats (many copies)
PGG PF13962 424 - 528 2.5e-18 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 127
Acc36I ACCTGC 1 cut(s) 1310
AccB7I CCANNNNNTGG 1 cut(s) 1516
AccBSI CCGCTC 1 cut(s) 976
AccI GTMKAC 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 776
AciI CCGC 8 cut(s) 24, 156, 242, 974, 1006, 1607, 1746, 1797
AclWI GGATC 2 cut(s) 12, 914
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 3 cut(s) 296, 1154, 1591
AcuI CTGAAG 2 cut(s) 836, 1581
AcyI GRCGYC 1 cut(s) 86
AfaI GTAC 2 cut(s) 649, 1032
AfiI CCNNNNNNNGG 6 cut(s) 300, 445, 570, 1364, 1468, 1516
AgsI TTSAA 5 cut(s) 323, 605, 687, 1066, 1171
AhdI GACNNNNNGTC 1 cut(s) 1057
AjnI CCWGG 3 cut(s) 245, 1189, 1548
AleI CACNNNNGTG 2 cut(s) 199, 441
AluBI AGCT 9 cut(s) 286, 619, 700, 758, 805, 985, 1232, 1247, 1394
AluI AGCT 9 cut(s) 286, 619, 700, 758, 805, 985, 1232, 1247, 1394
Alw21I GWGCWC 3 cut(s) 101, 1461, 1793
Alw26I GTCTC 6 cut(s) 309, 396, 500, 1251, 1643, 1770
Alw44I GTGCAC 1 cut(s) 1457
AlwI GGATC 2 cut(s) 12, 914
AlwNI CAGNNNCTG 1 cut(s) 1277
Aor13HI TCCGGA 1 cut(s) 776
AoxI GGCC 5 cut(s) 153, 243, 781, 1692, 1801
ApaLI GTGCAC 1 cut(s) 1457
ApeKI GCWGC 6 cut(s) 232, 560, 665, 770, 989, 1292
ApoI RAATTY 3 cut(s) 296, 1154, 1591
Asp700I GAANNNNTTC 1 cut(s) 1594
AspLEI GCGC 3 cut(s) 128, 232, 364
AspS9I GGNCC 3 cut(s) 333, 533, 1679
AsuHPI GGTGA 6 cut(s) 233, 428, 460, 650, 1564, 1785
AvaII GGWCC 3 cut(s) 333, 533, 1679
BaeGI GKGCMC 1 cut(s) 1461
BanII GRGCYC 1 cut(s) 307
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 474
Bbv12I GWGCWC 3 cut(s) 101, 1461, 1793
BbvI GCAGC 6 cut(s) 219, 547, 677, 782, 976, 1279
BccI CCATC 4 cut(s) 344, 529, 1430, 1510
BceAI ACGGC 4 cut(s) 548, 564, 895, 1565
BciT130I CCWGG 3 cut(s) 247, 1191, 1550
BclI TGATCA 3 cut(s) 378, 577, 1360
BcoDI GTCTC 6 cut(s) 309, 396, 500, 1251, 1643, 1770
BfaI CTAG 4 cut(s) 720, 1044, 1370, 1812
BfmI CTRYAG 2 cut(s) 264, 462
BfuAI ACCTGC 1 cut(s) 1310
BglII AGATCT 1 cut(s) 716
BisI GCNGC 9 cut(s) 24, 156, 233, 243, 561, 666, 771, 990, 1293
BlsI GCNGC 9 cut(s) 25, 157, 234, 244, 562, 667, 772, 991, 1294
BmcAI AGTACT 1 cut(s) 1032
Bme1390I CCNGG 3 cut(s) 247, 1191, 1550
Bme18I GGWCC 3 cut(s) 333, 533, 1679
BmeRI GACNNNNNGTC 1 cut(s) 1057
BmgT120I GGNCC 3 cut(s) 333, 533, 1679
BmiI GGNNCC 3 cut(s) 291, 306, 535
BmrFI CCNGG 3 cut(s) 247, 1191, 1550
BmrI ACTGGG 1 cut(s) 1338
BmsI GCATC 3 cut(s) 677, 782, 1030
BmuI ACTGGG 1 cut(s) 1338
BpiI GAAGAC 1 cut(s) 474
BplI GAGNNNNNCTC 2 cut(s) 742, 774
BpuEI CTTGAG 4 cut(s) 689, 1110, 1233, 1790
Bsa29I ATCGAT 1 cut(s) 1514
BsaAI YACGTR 1 cut(s) 118
BsaHI GRCGYC 1 cut(s) 86
BsaI GGTCTC 3 cut(s) 309, 1643, 1770
BsaJI CCNNGG 6 cut(s) 49, 439, 923, 1548, 1653, 1695
BsaWI WCCGGW 1 cut(s) 776
Bsc4I CCNNNNNNNGG 6 cut(s) 300, 445, 570, 1364, 1468, 1516
Bse1I ACTGG 2 cut(s) 1344, 1440
Bse3DI GCAATG 5 cut(s) 43, 135, 499, 1563, 1608
BseAI TCCGGA 1 cut(s) 776
BseBI CCWGG 3 cut(s) 247, 1191, 1550
BseCI ATCGAT 1 cut(s) 1514
BseDI CCNNGG 6 cut(s) 49, 439, 923, 1548, 1653, 1695
BseGI GGATG 4 cut(s) 336, 650, 773, 961
BseLI CCNNNNNNNGG 6 cut(s) 300, 445, 570, 1364, 1468, 1516
BseMI GCAATG 5 cut(s) 43, 135, 499, 1563, 1608
BseMII CTCAG 4 cut(s) 465, 909, 943, 1799
BseNI ACTGG 2 cut(s) 1344, 1440
BseRI GAGGAG 1 cut(s) 297
BseSI GKGCMC 1 cut(s) 1461
BseXI GCAGC 6 cut(s) 219, 547, 677, 782, 976, 1279
BseYI CCCAGC 1 cut(s) 1520
BsgI GTGCAG 2 cut(s) 218, 1278
BshFI GGCC 5 cut(s) 155, 245, 783, 1694, 1803
BshVI ATCGAT 1 cut(s) 1514
BsiHKAI GWGCWC 3 cut(s) 101, 1461, 1793
BsiSI CCGG 1 cut(s) 777
BslFI GGGAC 1 cut(s) 519
BslI CCNNNNNNNGG 6 cut(s) 300, 445, 570, 1364, 1468, 1516
BsmAI GTCTC 6 cut(s) 309, 396, 500, 1251, 1643, 1770
BsmFI GGGAC 1 cut(s) 519
BsmI GAATGC 1 cut(s) 821
BsnI GGCC 5 cut(s) 155, 245, 783, 1694, 1803
Bso31I GGTCTC 3 cut(s) 309, 1643, 1770
Bsp1286I GDGCHC 4 cut(s) 101, 307, 1461, 1793
Bsp13I TCCGGA 1 cut(s) 776
Bsp143I GATC 9 cut(s) 4, 174, 378, 577, 716, 841, 919, 1360, 1713
Bsp19I CCATGG 1 cut(s) 1653
BspACI CCGC 8 cut(s) 24, 156, 242, 974, 1006, 1607, 1746, 1797
BspANI GGCC 5 cut(s) 155, 245, 783, 1694, 1803
BspCNI CTCAG 4 cut(s) 466, 908, 942, 1798
BspDI ATCGAT 1 cut(s) 1514
BspEI TCCGGA 1 cut(s) 776
BspHI TCATGA 2 cut(s) 375, 1011
BspLI GGNNCC 3 cut(s) 291, 306, 535
BspMAI CTGCAG 1 cut(s) 466
BspMI ACCTGC 1 cut(s) 1310
BspPI GGATC 2 cut(s) 12, 914
BspTNI GGTCTC 3 cut(s) 309, 1643, 1770
BsrBI CCGCTC 1 cut(s) 976
BsrDI GCAATG 5 cut(s) 43, 135, 499, 1563, 1608
BsrI ACTGG 2 cut(s) 1344, 1440
BssECI CCNNGG 6 cut(s) 49, 439, 923, 1548, 1653, 1695
BssMI GATC 9 cut(s) 4, 174, 378, 577, 716, 841, 919, 1360, 1713
BssNAI GTATAC 1 cut(s) 16
BssNI GRCGYC 1 cut(s) 86
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 5 cut(s) 49, 439, 923, 1653, 1695
Bst1107I GTATAC 1 cut(s) 16
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 247, 1191, 1550
Bst4CI ACNGT 4 cut(s) 103, 201, 1262, 1309
Bst6I CTCTTC 2 cut(s) 498, 633
BstACI GRCGYC 1 cut(s) 86
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 6 cut(s) 124, 288, 558, 987, 1570, 1801
BstDEI CTNAG 7 cut(s) 93, 474, 895, 929, 1233, 1453, 1785
BstDSI CCRYGG 1 cut(s) 1653
BstF5I GGATG 4 cut(s) 336, 650, 773, 961
BstHHI GCGC 3 cut(s) 128, 232, 364
BstKTI GATC 9 cut(s) 7, 177, 381, 580, 719, 844, 922, 1363, 1716
BstMAI GTCTC 6 cut(s) 309, 396, 500, 1251, 1643, 1770
BstMBI GATC 9 cut(s) 4, 174, 378, 577, 716, 841, 919, 1360, 1713
BstMWI GCNNNNNNNGC 6 cut(s) 557, 982, 1301, 1544, 1565, 1743
BstNI CCWGG 3 cut(s) 247, 1191, 1550
BstNSI RCATGY 2 cut(s) 126, 560
BstSCI CCNGG 3 cut(s) 245, 1189, 1548
BstSFI CTRYAG 2 cut(s) 264, 462
BstSLI GKGCMC 1 cut(s) 1461
BstSNI TACGTA 1 cut(s) 118
BstV1I GCAGC 6 cut(s) 219, 547, 677, 782, 976, 1279
BstV2I GAAGAC 1 cut(s) 474
BstX2I RGATCY 3 cut(s) 4, 716, 919
BstXI CCANNNNNNTGG 1 cut(s) 201
BstYI RGATCY 3 cut(s) 4, 716, 919
BstZ17I GTATAC 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 1514
BsuRI GGCC 5 cut(s) 155, 245, 783, 1694, 1803
BsuTUI ATCGAT 1 cut(s) 1514
BtgI CCRYGG 1 cut(s) 1653
BtgZI GCGATG 1 cut(s) 1408
BtsCI GGATG 4 cut(s) 336, 650, 773, 961
BtsI GCAGTG 2 cut(s) 225, 429
BtsIMutI CAGTG 6 cut(s) 225, 429, 937, 1295, 1351, 1793
BveI ACCTGC 1 cut(s) 1310
Cac8I GCNNGC 6 cut(s) 124, 288, 558, 987, 1570, 1801
CaiI CAGNNNCTG 1 cut(s) 1277
CciI TCATGA 2 cut(s) 375, 1011
CfoI GCGC 3 cut(s) 128, 232, 364
Cfr13I GGNCC 3 cut(s) 333, 533, 1679
ClaI ATCGAT 1 cut(s) 1514
CseI GACGC 2 cut(s) 94, 1438
Csp6I GTAC 2 cut(s) 648, 1031
CspCI CAANNNNNGTGG 2 cut(s) 1671, 1706
CviQI GTAC 2 cut(s) 648, 1031
DdeI CTNAG 7 cut(s) 93, 474, 895, 929, 1233, 1453, 1785
DpnI GATC 9 cut(s) 6, 176, 380, 579, 718, 843, 921, 1362, 1715
DpnII GATC 9 cut(s) 4, 174, 378, 577, 716, 841, 919, 1360, 1713
DraI TTTAAA 1 cut(s) 915
DriI GACNNNNNGTC 1 cut(s) 1057
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 498, 633
Eam1105I GACNNNNNGTC 1 cut(s) 1057
EarI CTCTTC 2 cut(s) 498, 633
Eco105I TACGTA 1 cut(s) 118
Eco130I CCWWGG 5 cut(s) 49, 439, 923, 1653, 1695
Eco24I GRGCYC 1 cut(s) 307
Eco31I GGTCTC 3 cut(s) 309, 1643, 1770
Eco47I GGWCC 3 cut(s) 333, 533, 1679
Eco57I CTGAAG 2 cut(s) 836, 1581
EcoO109I RGGNCCY 1 cut(s) 533
EcoRII CCWGG 3 cut(s) 245, 1189, 1548
EcoT14I CCWWGG 5 cut(s) 49, 439, 923, 1653, 1695
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 5 cut(s) 49, 439, 923, 1653, 1695
FalI AAGNNNNNCTT 6 cut(s) 54, 86, 535, 567, 1378, 1410
FaqI GGGAC 1 cut(s) 519
FauI CCCGC 1 cut(s) 1804
FauNDI CATATG 2 cut(s) 569, 1576
FbaI TGATCA 3 cut(s) 378, 577, 1360
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 9 cut(s) 24, 156, 233, 243, 561, 666, 771, 990, 1293
FokI GGATG 4 cut(s) 323, 657, 760, 968
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 9 cut(s) 24, 156, 233, 243, 561, 666, 771, 990, 1293
FspBI CTAG 4 cut(s) 720, 1044, 1370, 1812
FspI TGCGCA 1 cut(s) 127
GlaI GCGC 3 cut(s) 127, 231, 363
GluI GCNGC 9 cut(s) 24, 156, 233, 243, 561, 666, 771, 990, 1293
GsaI CCCAGC 1 cut(s) 1524
HaeIII GGCC 5 cut(s) 155, 245, 783, 1694, 1803
HapII CCGG 1 cut(s) 777
HgaI GACGC 2 cut(s) 94, 1438
HhaI GCGC 3 cut(s) 128, 232, 364
Hin1I GRCGYC 1 cut(s) 86
Hin6I GCGC 3 cut(s) 126, 230, 362
HinP1I GCGC 3 cut(s) 126, 230, 362
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HinfI GANTC 3 cut(s) 1274, 1671, 1758
HpaII CCGG 1 cut(s) 777
HphI GGTGA 6 cut(s) 233, 428, 460, 650, 1564, 1785
Hpy166II GTNNAC 4 cut(s) 16, 138, 191, 1459
Hpy188I TCNGA 3 cut(s) 855, 1501, 1775
Hpy188III TCNNGA 9 cut(s) 74, 146, 169, 376, 581, 777, 1012, 1250, 1399
Hpy8I GTNNAC 4 cut(s) 16, 138, 191, 1459
HpyAV CCTTC 5 cut(s) 26, 1247, 1380, 1570, 1670
HpyCH4III ACNGT 4 cut(s) 103, 201, 1262, 1309
HpyCH4IV ACGT 3 cut(s) 117, 237, 274
HpyF10VI GCNNNNNNNGC 6 cut(s) 557, 982, 1301, 1544, 1565, 1743
HpyF3I CTNAG 7 cut(s) 93, 474, 895, 929, 1233, 1453, 1785
HpySE526I ACGT 3 cut(s) 117, 237, 274
Hsp92I GRCGYC 1 cut(s) 86
HspAI GCGC 3 cut(s) 126, 230, 362
Kpn2I TCCGGA 1 cut(s) 776
Ksp22I TGATCA 3 cut(s) 378, 577, 1360
Kzo9I GATC 9 cut(s) 4, 174, 378, 577, 716, 841, 919, 1360, 1713
LmnI GCTCC 4 cut(s) 295, 310, 616, 981
Lsp1109I GCAGC 6 cut(s) 219, 547, 677, 782, 976, 1279
LweI GCATC 3 cut(s) 677, 782, 1030
MaeI CTAG 4 cut(s) 720, 1044, 1370, 1812
MaeII ACGT 3 cut(s) 117, 237, 274
MaeIII GTNAC 1 cut(s) 221
MalI GATC 9 cut(s) 6, 176, 380, 579, 718, 843, 921, 1362, 1715
MbiI CCGCTC 1 cut(s) 976
MboI GATC 9 cut(s) 4, 174, 378, 577, 716, 841, 919, 1360, 1713
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 3 cut(s) 4, 716, 919
MhlI GDGCHC 4 cut(s) 101, 307, 1461, 1793
MlyI GAGTC 1 cut(s) 1752
MroI TCCGGA 1 cut(s) 776
MroXI GAANNNNTTC 1 cut(s) 1594
MseI TTAA 6 cut(s) 9, 390, 432, 914, 944, 1136
MslI CAYNNNNRTG 4 cut(s) 199, 441, 789, 1427
MspA1I CMGCKG 1 cut(s) 1799
MspI CCGG 1 cut(s) 777
MspR9I CCNGG 3 cut(s) 247, 1191, 1550
MunI CAATTG 1 cut(s) 687
Mva1269I GAATGC 1 cut(s) 821
MvaI CCWGG 3 cut(s) 247, 1191, 1550
MwoI GCNNNNNNNGC 6 cut(s) 557, 982, 1301, 1544, 1565, 1743
NcoI CCATGG 1 cut(s) 1653
NdeI CATATG 2 cut(s) 569, 1576
NdeII GATC 9 cut(s) 4, 174, 378, 577, 716, 841, 919, 1360, 1713
NlaIV GGNNCC 3 cut(s) 291, 306, 535
NmuCI GTSAC 1 cut(s) 221
NsbI TGCGCA 1 cut(s) 127
NspI RCATGY 2 cut(s) 126, 560
OliI CACNNNNGTG 2 cut(s) 199, 441
PaeI GCATGC 2 cut(s) 126, 560
PagI TCATGA 2 cut(s) 375, 1011
PctI GAATGC 1 cut(s) 821
PdmI GAANNNNTTC 1 cut(s) 1594
PfeI GAWTC 2 cut(s) 1274, 1671
PflFI GACNNNGTC 1 cut(s) 1435
PflMI CCANNNNNTGG 1 cut(s) 1516
PkrI GCNGC 9 cut(s) 25, 157, 234, 244, 562, 667, 772, 991, 1294
PleI GAGTC 1 cut(s) 1752
PpsI GAGTC 1 cut(s) 1752
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 533
Psp5II RGGWCCY 1 cut(s) 533
Psp6I CCWGG 3 cut(s) 245, 1189, 1548
PspFI CCCAGC 1 cut(s) 1520
PspGI CCWGG 3 cut(s) 245, 1189, 1548
PspN4I GGNNCC 3 cut(s) 291, 306, 535
PspPI GGNCC 3 cut(s) 333, 533, 1679
PspPPI RGGWCCY 1 cut(s) 533
PstI CTGCAG 1 cut(s) 466
PstNI CAGNNNCTG 1 cut(s) 1277
PsuI RGATCY 3 cut(s) 4, 716, 919
PsyI GACNNNGTC 1 cut(s) 1435
RsaI GTAC 2 cut(s) 649, 1032
RsaNI GTAC 2 cut(s) 648, 1031
RseI CAYNNNNRTG 4 cut(s) 199, 441, 789, 1427
SaqAI TTAA 6 cut(s) 9, 390, 432, 914, 944, 1136
SatI GCNGC 9 cut(s) 24, 156, 233, 243, 561, 666, 771, 990, 1293
Sau3AI GATC 9 cut(s) 4, 174, 378, 577, 716, 841, 919, 1360, 1713
Sau96I GGNCC 3 cut(s) 333, 533, 1679
ScaI AGTACT 1 cut(s) 1032
SchI GAGTC 1 cut(s) 1752
ScrFI CCNGG 3 cut(s) 247, 1191, 1550
SduI GDGCHC 4 cut(s) 101, 307, 1461, 1793
SfaNI GCATC 3 cut(s) 677, 782, 1030
SfcI CTRYAG 2 cut(s) 264, 462
SinI GGWCC 3 cut(s) 333, 533, 1679
SmiMI CAYNNNNRTG 4 cut(s) 199, 441, 789, 1427
SmlI CTYRAG 4 cut(s) 704, 1125, 1248, 1805
SmoI CTYRAG 4 cut(s) 704, 1125, 1248, 1805
SnaBI TACGTA 1 cut(s) 118
SphI GCATGC 2 cut(s) 126, 560
SsiI CCGC 8 cut(s) 24, 156, 242, 974, 1006, 1607, 1746, 1797
SspI AATATT 2 cut(s) 81, 1769
SspMI CTAG 4 cut(s) 720, 1044, 1370, 1812
StyD4I CCNGG 3 cut(s) 245, 1189, 1548
StyI CCWWGG 5 cut(s) 49, 439, 923, 1653, 1695
TaaI ACNGT 4 cut(s) 103, 201, 1262, 1309
TaiI ACGT 3 cut(s) 120, 240, 277
TaqI TCGA 5 cut(s) 168, 840, 1198, 1514, 1674
TatI WGTACW 2 cut(s) 647, 1030
TauI GCSGC 3 cut(s) 26, 158, 245
TfiI GAWTC 2 cut(s) 1274, 1671
Tru1I TTAA 6 cut(s) 9, 390, 432, 914, 944, 1136
Tru9I TTAA 6 cut(s) 9, 390, 432, 914, 944, 1136
TscAI CASTG 6 cut(s) 232, 429, 937, 1302, 1351, 1793
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 6 cut(s) 232, 560, 665, 770, 989, 1292
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 8 cut(s) 361, 651, 801, 812, 972, 1000, 1257, 1552
TspGWI ACGGA 2 cut(s) 267, 1189
TspRI CASTG 6 cut(s) 232, 429, 937, 1302, 1351, 1793
Tth111I GACNNNGTC 1 cut(s) 1435
Van91I CCANNNNNTGG 1 cut(s) 1516
VneI GTGCAC 1 cut(s) 1457
VpaK11BI GGWCC 3 cut(s) 333, 533, 1679
XapI RAATTY 3 cut(s) 296, 1154, 1591
XceI RCATGY 2 cut(s) 126, 560
XcmI CCANNNNNNNNNTGG 2 cut(s) 198, 1517
XmiI GTMKAC 1 cut(s) 15
XmnI GAANNNNTTC 1 cut(s) 1594
XspI CTAG 4 cut(s) 720, 1044, 1370, 1812
ZrmI AGTACT 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.