Rroxscaffold_3G00245990

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000489
Physical Location & Seq
Reverse (-)
39090370 .. 39096383
6014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00245990.1

Sequence Viewer

Length: 1923 bp
ATGAATCCCTGTGATTCAGCTGAGACGGAGAGTAGTCCAACATCGGCCTATCATGCCATGATACTGGAGGAGACCAACGCAATGTTCTGTTCTGAATGGAACCAGCCAAAATCTATACCAGTCATGAAAAGCTTAAGCGGCATGGATACTAAGGTGTTCAAGGAGGCACGAGAGGGCAATATTGATGCCCTTAGAGAACATAGTGATTATCTTCACCAGATATTGACTCCAACCAAAAACACAGTCCTCCATGTCTATATAGCTTGCGTAGGCAGTGCAAGGTTGACAGAGTCTGAAGAATTGCTGAAGTCAGCCGAGGCTGTAAGGGAGATGCTTAAAATGTGCCAGCGGCTACTATTGCAGCCAAACGAGAGCGGTGATACTGTCTTACACTTAGCGGCAAGACACGGACGTGCTGACATAGTTGGAGTTCTTATCCAGGCTGCAAAAGACAGGCATGGTGACCTCGAGGAAGGTATCACATCAAAAGAAGTATGCTACCAGTTTCTCATAAGAAGACCTAACGAGGAGAAAAACACAGCCTTGCACGAGGCAGTGCGGTTTAATCATCTAGATGTGGTAAAGATATTGACTGGGGAAGACCCTGAGTTTTTGTACTCTGCTAATGATGCTGGCGAAACTCCAGTATACATGGCTGCCGAAAGAGGATATGGCAAATTGGTTTTTGAAATCCTTGACACCTGCACAAGTTCATCCTACCAAGGACCCGATGGTTTTACAGCTCTACACGTTGCAGCTGCCTACGGTCATGATCAAATAACGAGAAGATTACTAGAGAAGGAAATGACGCTAGCAACAGCAGCAGACGGAGAAGGATATACTCCACTTCATATGGCTGCATATAGGGGTCACGTTTCAATTGTGAAACAAATACTTGAATGTGACAAGTCCACTGCCTACATTGGCGACATTATTGACAAGATGACACCCGTTCACTATGCAGCCTATGCAGGGCATGTAGATGTACTAAAACTGCTTATTTTCCACTGCCCTGACAGTTTCGAATTGGTGGACAGTGAAGGTCTCAATGCCCTACATTTAGCAATTATCGGTAATCAATATAAAGTAGAAGAGTTTGTTCGAAAAGATCCATGGCTTAGTAGCGTCCTTTTAAATAGCAAAGATTCTGTCGGGAACACACCTCTCCATCAAATTGCTACTTCTGAAAAATATGAAGGCTTGAAATTTCTCAGTGATTCGAGAGTTGATAAGATGGCATTCAATAAAAAAAATAAGAACGCGCTAGACATCATTCTAAAGAGCACGAATCCTCAGTGGAAGAGAAATCTCTATAAAGGTTTGAAAAAGTCTGGTGCAAGAGTAGGTCAACGAAAGTCAAGAGACAAAGATGGTGGCACGAAAGATCATGCAAGTAAAGGTGACAAAGAGTCAAAATACAAGGACATAAAAGAATCTCATTTGGTAGTGTCTGCACTCATAGCAACTGTAACCTTCGCAGCAGGCTTCACTGTGCCTGGTGGTTATCAAAGTGAAAAAGGGCCGGATCAAGGTTTTGCGGTTCTATCCAGAAATGCAGCTTTCAAAGCATTTGTGATAACAAATACATTAGCCATGACTCTGTCCAGTTGTGCCGTCATGATACGCTTTTTCTTGTCACTAAAAGGGGAACATGGGTCAAGTTCATTATCTATGGCAATAAATTGTACATTTCTCGCCCTGTTTGCAATGGTGGCTGCATTCCTTACCGGTGCATATGCAGTATTAGGTGGTCGTTCTTCATTAGGACTTGCTATTGCGGTTTGTGTGCTCGGCAGCGTCTTCTTCTCGGTTTTGAGTTTTGTTTCCATTTCTCCAGCTTTCAATACTTTTCGTAAGCGTGCCTCTCATCTCCGAGGCCGGATAGTTGAACGGCTCGGATTGGAGTATTATTTTTTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

640

Amino Acids

70.57

Weight (kDa)

7.08

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 110 - 198 2.8e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 168 - 230 3.8e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 218 - 302 7e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 249 - 296 6.5e-10 Ankyrin repeats (many copies)
Ank_2 PF12796 296 - 363 4.9e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 319 - 412 9.3e-10 Ankyrin repeats (3 copies)
PGG PF13962 471 - 581 1.4e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 710
AbsI CCTCGAGG 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 710
AccBSI CCGCTC 1 cut(s) 375
AccI GTMKAC 1 cut(s) 648
AccII CGCG 1 cut(s) 1262
AciI CCGC 7 cut(s) 138, 349, 375, 398, 559, 1538, 1778
AclWI GGATC 2 cut(s) 1103, 1533
AcsI RAATTY 1 cut(s) 1205
AcuI CTGAAG 2 cut(s) 315, 326
AfaI GTAC 3 cut(s) 617, 987, 1687
AfiI CCNNNNNNNGG 2 cut(s) 972, 1529
AflII CTTAAG 1 cut(s) 133
AflIII ACRYGT 1 cut(s) 748
AgeI ACCGGT 1 cut(s) 1727
AhdI GACNNNNNGTC 1 cut(s) 1408
AjiI CACGTC 1 cut(s) 413
AjnI CCWGG 2 cut(s) 438, 1495
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AleI CACNNNNGTG 1 cut(s) 411
AluBI AGCT 7 cut(s) 20, 132, 263, 743, 758, 1559, 1838
AluI AGCT 7 cut(s) 20, 132, 263, 743, 758, 1559, 1838
Alw21I GWGCWC 2 cut(s) 1286, 1791
Alw26I GTCTC 4 cut(s) 17, 65, 1049, 1356
AlwI GGATC 2 cut(s) 1103, 1533
AlwNI CAGNNNCTG 1 cut(s) 293
Ama87I CYCGRG 1 cut(s) 467
AoxI GGCC 3 cut(s) 45, 1520, 1876
ApoI RAATTY 1 cut(s) 1205
ArsI GACNNNNNNTTYG 4 cut(s) 228, 260, 1134, 1166
AsiGI ACCGGT 1 cut(s) 1727
AspLEI GCGC 1 cut(s) 1264
AspS9I GGNCC 2 cut(s) 725, 1520
AsuHPI GGTGA 4 cut(s) 206, 389, 473, 1412
AsuII TTCGAA 2 cut(s) 1023, 1102
AsuNHI GCTAGC 1 cut(s) 811
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 725
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BauI CACGAG 2 cut(s) 168, 548
BbsI GAAGAC 3 cut(s) 523, 606, 1792
Bbv12I GWGCWC 2 cut(s) 1286, 1791
BccI CCATC 4 cut(s) 725, 1176, 1228, 1364
BceAI ACGGC 2 cut(s) 1598, 1907
BciT130I CCWGG 2 cut(s) 440, 1497
BciVI GTATCC 1 cut(s) 139
BclI TGATCA 1 cut(s) 772
BcoDI GTCTC 4 cut(s) 17, 65, 1049, 1356
BfaI CTAG 4 cut(s) 572, 794, 812, 1265
BfrI CTTAAG 1 cut(s) 133
BfuAI ACCTGC 1 cut(s) 710
BfuI GTATCC 1 cut(s) 139
Bme1390I CCNGG 2 cut(s) 440, 1497
Bme18I GGWCC 1 cut(s) 725
BmeRI GACNNNNNGTC 1 cut(s) 1408
BmeT110I CYCGRG 1 cut(s) 467
BmgBI CACGTC 1 cut(s) 413
BmgT120I GGNCC 2 cut(s) 725, 1520
BmiI GGNNCC 2 cut(s) 101, 727
BmrFI CCNGG 2 cut(s) 440, 1497
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 3 cut(s) 175, 321, 619
BmtI GCTAGC 1 cut(s) 815
BmuI ACTGGG 1 cut(s) 603
BpiI GAAGAC 3 cut(s) 523, 606, 1792
BpmI CTGGAG 3 cut(s) 86, 627, 1818
Bpu14I TTCGAA 2 cut(s) 1023, 1102
BsaI GGTCTC 2 cut(s) 65, 1049
BsaJI CCNNGG 4 cut(s) 315, 721, 1112, 1873
BsaWI WCCGGW 1 cut(s) 1727
Bsc4I CCNNNNNNNGG 2 cut(s) 972, 1529
Bse118I RCCGGY 1 cut(s) 1727
Bse1I ACTGG 6 cut(s) 69, 119, 502, 598, 644, 1605
Bse3DI GCAATG 2 cut(s) 87, 1713
BseBI CCWGG 2 cut(s) 440, 1497
BseDI CCNNGG 4 cut(s) 315, 721, 1112, 1873
BseGI GGATG 1 cut(s) 713
BseLI CCNNNNNNNGG 2 cut(s) 972, 1529
BseMI GCAATG 2 cut(s) 87, 1713
BseMII CTCAG 4 cut(s) 12, 597, 1225, 1307
BseNI ACTGG 6 cut(s) 69, 119, 502, 598, 644, 1605
BseRI GAGGAG 2 cut(s) 83, 542
BsgI GTGCAG 2 cut(s) 688, 1437
Bsh1236I CGCG 1 cut(s) 1262
BshFI GGCC 3 cut(s) 47, 1522, 1878
BshTI ACCGGT 1 cut(s) 1727
BsiHKAI GWGCWC 2 cut(s) 1286, 1791
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 3 cut(s) 1523, 1728, 1879
BslI CCNNNNNNNGG 2 cut(s) 972, 1529
BsmAI GTCTC 4 cut(s) 17, 65, 1049, 1356
BsmBI CGTCTC 1 cut(s) 17
BsmI GAATGC 2 cut(s) 1238, 1718
BsnI GGCC 3 cut(s) 47, 1522, 1878
Bso31I GGTCTC 2 cut(s) 65, 1049
BsoBI CYCGRG 1 cut(s) 467
Bsp119I TTCGAA 2 cut(s) 1023, 1102
Bsp1286I GDGCHC 2 cut(s) 1286, 1791
Bsp1407I TGTACA 1 cut(s) 1685
Bsp143I GATC 4 cut(s) 772, 1108, 1384, 1525
Bsp19I CCATGG 1 cut(s) 1112
BspACI CCGC 7 cut(s) 138, 349, 375, 398, 559, 1538, 1778
BspANI GGCC 3 cut(s) 47, 1522, 1878
BspCNI CTCAG 4 cut(s) 13, 598, 1224, 1306
BspFNI CGCG 1 cut(s) 1262
BspHI TCATGA 3 cut(s) 123, 769, 1617
BspLI GGNNCC 2 cut(s) 101, 727
BspMI ACCTGC 1 cut(s) 710
BspOI GCTAGC 1 cut(s) 815
BspPI GGATC 2 cut(s) 1103, 1533
BspT104I TTCGAA 2 cut(s) 1023, 1102
BspTI CTTAAG 1 cut(s) 133
BspTNI GGTCTC 2 cut(s) 65, 1049
BsrBI CCGCTC 1 cut(s) 375
BsrDI GCAATG 2 cut(s) 87, 1713
BsrFI RCCGGY 1 cut(s) 1727
BsrGI TGTACA 1 cut(s) 1685
BsrI ACTGG 6 cut(s) 69, 119, 502, 598, 644, 1605
BssAI RCCGGY 1 cut(s) 1727
BssECI CCNNGG 4 cut(s) 315, 721, 1112, 1873
BssMI GATC 4 cut(s) 772, 1108, 1384, 1525
BssNAI GTATAC 1 cut(s) 649
BssSI CACGAG 2 cut(s) 168, 548
BssT1I CCWWGG 2 cut(s) 721, 1112
Bst1107I GTATAC 1 cut(s) 649
Bst2BI CACGAG 2 cut(s) 168, 548
Bst2UI CCWGG 2 cut(s) 440, 1497
Bst4CI ACNGT 7 cut(s) 244, 385, 767, 1019, 1037, 1468, 1492
Bst6I CTCTTC 2 cut(s) 1086, 1295
BstAFI CTTAAG 1 cut(s) 133
BstAPI GCANNNNNTGC 1 cut(s) 968
BstAUI TGTACA 1 cut(s) 1685
BstBI TTCGAA 2 cut(s) 1023, 1102
BstC8I GCNNGC 6 cut(s) 265, 347, 634, 813, 1483, 1860
BstDEI CTNAG 8 cut(s) 21, 150, 191, 394, 606, 1118, 1211, 1293
BstDSI CCRYGG 1 cut(s) 1112
BstEII GGTNACC 1 cut(s) 461
BstF5I GGATG 1 cut(s) 713
BstFNI CGCG 1 cut(s) 1262
BstHHI GCGC 1 cut(s) 1264
BstKTI GATC 4 cut(s) 775, 1111, 1387, 1528
BstMAI GTCTC 4 cut(s) 17, 65, 1049, 1356
BstMBI GATC 4 cut(s) 772, 1108, 1384, 1525
BstNI CCWGG 2 cut(s) 440, 1497
BstNSI RCATGY 1 cut(s) 980
BstPI GGTNACC 1 cut(s) 461
BstSCI CCNGG 2 cut(s) 438, 1495
BstUI CGCG 1 cut(s) 1262
BstV2I GAAGAC 3 cut(s) 523, 606, 1792
BstX2I RGATCY 1 cut(s) 1108
BstXI CCANNNNNNTGG 1 cut(s) 64
BstYI RGATCY 1 cut(s) 1108
BstZ17I GTATAC 1 cut(s) 649
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 3 cut(s) 47, 1522, 1878
BtgI CCRYGG 1 cut(s) 1112
BtrI CACGTC 1 cut(s) 413
BtsCI GGATG 1 cut(s) 713
BtsI GCAGTG 4 cut(s) 280, 561, 912, 1006
BtsIMutI CAGTG 8 cut(s) 280, 561, 912, 1006, 1042, 1219, 1301, 1488
BveI ACCTGC 1 cut(s) 710
Cac8I GCNNGC 6 cut(s) 265, 347, 634, 813, 1483, 1860
CaiI CAGNNNCTG 1 cut(s) 293
CciI TCATGA 3 cut(s) 123, 769, 1617
CfoI GCGC 1 cut(s) 1264
Cfr10I RCCGGY 1 cut(s) 1727
Cfr13I GGNCC 2 cut(s) 725, 1520
CseI GACGC 3 cut(s) 817, 1114, 1786
Csp6I GTAC 3 cut(s) 616, 986, 1686
CspAI ACCGGT 1 cut(s) 1727
CspCI CAANNNNNGTGG 2 cut(s) 1354, 1389
CviQI GTAC 3 cut(s) 616, 986, 1686
DdeI CTNAG 8 cut(s) 21, 150, 191, 394, 606, 1118, 1211, 1293
DpnI GATC 4 cut(s) 774, 1110, 1386, 1527
DpnII GATC 4 cut(s) 772, 1108, 1384, 1525
DraI TTTAAA 1 cut(s) 1134
DriI GACNNNNNGTC 1 cut(s) 1408
Eam1104I CTCTTC 2 cut(s) 1086, 1295
Eam1105I GACNNNNNGTC 1 cut(s) 1408
EarI CTCTTC 2 cut(s) 1086, 1295
Eco130I CCWWGG 2 cut(s) 721, 1112
Eco31I GGTCTC 2 cut(s) 65, 1049
Eco47I GGWCC 1 cut(s) 725
Eco57I CTGAAG 2 cut(s) 315, 326
Eco88I CYCGRG 1 cut(s) 467
Eco91I GGTNACC 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 725
EcoO65I GGTNACC 1 cut(s) 461
EcoRII CCWGG 2 cut(s) 438, 1495
EcoT14I CCWWGG 2 cut(s) 721, 1112
ErhI CCWWGG 2 cut(s) 721, 1112
Esp3I CGTCTC 1 cut(s) 17
FauNDI CATATG 2 cut(s) 852, 1735
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 1 cut(s) 648
FokI GGATG 1 cut(s) 700
FspBI CTAG 4 cut(s) 572, 794, 812, 1265
GlaI GCGC 1 cut(s) 1263
GsuI CTGGAG 3 cut(s) 86, 627, 1818
HaeIII GGCC 3 cut(s) 47, 1522, 1878
HapII CCGG 3 cut(s) 1523, 1728, 1879
HgaI GACGC 3 cut(s) 817, 1114, 1786
HhaI GCGC 1 cut(s) 1264
Hin6I GCGC 1 cut(s) 1262
HinP1I GCGC 1 cut(s) 1262
HincII GTYRAC 2 cut(s) 285, 1349
HindII GTYRAC 2 cut(s) 285, 1349
HindIII AAGCTT 1 cut(s) 130
HpaII CCGG 3 cut(s) 1523, 1728, 1879
HphI GGTGA 4 cut(s) 206, 389, 473, 1412
Hpy166II GTNNAC 6 cut(s) 285, 649, 912, 955, 1033, 1349
Hpy188I TCNGA 5 cut(s) 94, 295, 1186, 1874, 1898
Hpy188III TCNNGA 8 cut(s) 124, 572, 770, 1153, 1221, 1359, 1548, 1618
Hpy8I GTNNAC 6 cut(s) 285, 649, 912, 955, 1033, 1349
HpyAV CCTTC 6 cut(s) 467, 793, 827, 1034, 1190, 1483
HpyCH4III ACNGT 7 cut(s) 244, 385, 767, 1019, 1037, 1468, 1492
HpyCH4IV ACGT 3 cut(s) 412, 750, 873
HpyF3I CTNAG 8 cut(s) 21, 150, 191, 394, 606, 1118, 1211, 1293
HpySE526I ACGT 3 cut(s) 412, 750, 873
HspAI GCGC 1 cut(s) 1262
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 4 cut(s) 772, 1108, 1384, 1525
LweI GCATC 3 cut(s) 175, 321, 619
MaeI CTAG 4 cut(s) 572, 794, 812, 1265
MaeII ACGT 3 cut(s) 412, 750, 873
MaeIII GTNAC 6 cut(s) 461, 869, 902, 1400, 1468, 1635
MalI GATC 4 cut(s) 774, 1110, 1386, 1527
MbiI CCGCTC 1 cut(s) 375
MboI GATC 4 cut(s) 772, 1108, 1384, 1525
MfeI CAATTG 1 cut(s) 879
MflI RGATCY 1 cut(s) 1108
MhlI GDGCHC 2 cut(s) 1286, 1791
MluCI AATT 8 cut(s) 299, 677, 879, 1025, 1065, 1173, 1205, 1681
MlyI GAGTC 4 cut(s) 220, 299, 1418, 1591
MmeI TCCRAC 3 cut(s) 62, 254, 406
MseI TTAA 4 cut(s) 134, 336, 564, 1133
MslI CAYNNNNRTG 3 cut(s) 411, 573, 981
MspA1I CMGCKG 3 cut(s) 20, 349, 758
MspCI CTTAAG 1 cut(s) 133
MspI CCGG 3 cut(s) 1523, 1728, 1879
MspR9I CCNGG 2 cut(s) 440, 1497
MunI CAATTG 1 cut(s) 879
Mva1269I GAATGC 2 cut(s) 1238, 1718
MvaI CCWGG 2 cut(s) 440, 1497
MvnI CGCG 1 cut(s) 1262
NcoI CCATGG 1 cut(s) 1112
NdeI CATATG 2 cut(s) 852, 1735
NdeII GATC 4 cut(s) 772, 1108, 1384, 1525
NheI GCTAGC 1 cut(s) 811
NlaIV GGNNCC 2 cut(s) 101, 727
NmeAIII GCCGAG 2 cut(s) 340, 1770
NmuCI GTSAC 5 cut(s) 461, 869, 902, 1400, 1635
NspI RCATGY 1 cut(s) 980
NspV TTCGAA 2 cut(s) 1023, 1102
OliI CACNNNNGTG 1 cut(s) 411
PaeR7I CTCGAG 1 cut(s) 467
PagI TCATGA 3 cut(s) 123, 769, 1617
PaqCI CACCTGC 1 cut(s) 710
PctI GAATGC 2 cut(s) 1238, 1718
PfeI GAWTC 6 cut(s) 4, 14, 1145, 1217, 1288, 1433
PflFI GACNNNGTC 2 cut(s) 289, 1600
PinAI ACCGGT 1 cut(s) 1727
PleI GAGTC 4 cut(s) 220, 298, 1417, 1591
PpsI GAGTC 4 cut(s) 220, 298, 1417, 1591
PpuMI RGGWCCY 1 cut(s) 725
Psp5II RGGWCCY 1 cut(s) 725
Psp6I CCWGG 2 cut(s) 438, 1495
PspEI GGTNACC 1 cut(s) 461
PspGI CCWGG 2 cut(s) 438, 1495
PspN4I GGNNCC 2 cut(s) 101, 727
PspPI GGNCC 2 cut(s) 725, 1520
PspPPI RGGWCCY 1 cut(s) 725
PspXI VCTCGAGB 1 cut(s) 467
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 1 cut(s) 1108
PsyI GACNNNGTC 2 cut(s) 289, 1600
PvuII CAGCTG 2 cut(s) 20, 758
RsaI GTAC 3 cut(s) 617, 987, 1687
RsaNI GTAC 3 cut(s) 616, 986, 1686
RseI CAYNNNNRTG 3 cut(s) 411, 573, 981
SaqAI TTAA 4 cut(s) 134, 336, 564, 1133
Sau3AI GATC 4 cut(s) 772, 1108, 1384, 1525
Sau96I GGNCC 2 cut(s) 725, 1520
SchI GAGTC 4 cut(s) 220, 299, 1418, 1591
ScrFI CCNGG 2 cut(s) 440, 1497
SduI GDGCHC 2 cut(s) 1286, 1791
SfaNI GCATC 3 cut(s) 175, 321, 619
Sfr274I CTCGAG 1 cut(s) 467
SfuI TTCGAA 2 cut(s) 1023, 1102
SinI GGWCC 1 cut(s) 725
SlaI CTCGAG 1 cut(s) 467
SmiMI CAYNNNNRTG 3 cut(s) 411, 573, 981
SmlI CTYRAG 2 cut(s) 133, 467
SmoI CTYRAG 2 cut(s) 133, 467
Sse9I AATT 8 cut(s) 299, 677, 879, 1025, 1065, 1173, 1205, 1681
SsiI CCGC 7 cut(s) 138, 349, 375, 398, 559, 1538, 1778
SspI AATATT 1 cut(s) 181
SspMI CTAG 4 cut(s) 572, 794, 812, 1265
StyD4I CCNGG 2 cut(s) 438, 1495
StyI CCWWGG 2 cut(s) 721, 1112
TaaI ACNGT 7 cut(s) 244, 385, 767, 1019, 1037, 1468, 1492
TaiI ACGT 3 cut(s) 415, 753, 876
TaqI TCGA 4 cut(s) 468, 1023, 1102, 1220
TasI AATT 8 cut(s) 299, 677, 879, 1025, 1065, 1173, 1205, 1681
TatI WGTACW 3 cut(s) 615, 985, 1685
TauI GCSGC 3 cut(s) 141, 352, 401
TfiI GAWTC 6 cut(s) 4, 14, 1145, 1217, 1288, 1433
Tru1I TTAA 4 cut(s) 134, 336, 564, 1133
Tru9I TTAA 4 cut(s) 134, 336, 564, 1133
TscAI CASTG 8 cut(s) 280, 561, 919, 1013, 1042, 1219, 1301, 1495
TseFI GTSAC 5 cut(s) 461, 869, 902, 1400, 1635
Tsp45I GTSAC 5 cut(s) 461, 869, 902, 1400, 1635
TspDTI ATGAA 7 cut(s) 17, 140, 702, 839, 1209, 1653, 1749
TspGWI ACGGA 3 cut(s) 41, 423, 843
TspRI CASTG 8 cut(s) 280, 561, 919, 1013, 1042, 1219, 1301, 1495
Tth111I GACNNNGTC 2 cut(s) 289, 1600
Vha464I CTTAAG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 725
XapI RAATTY 1 cut(s) 1205
XbaI TCTAGA 1 cut(s) 571
XceI RCATGY 1 cut(s) 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XhoI CTCGAG 1 cut(s) 467
XmiI GTMKAC 1 cut(s) 648
XspI CTAG 4 cut(s) 572, 794, 812, 1265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.