Rw1G011650

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000489
Physical Location & Seq
Forward (+)
26096173 .. 26113948
17776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011650.1

Sequence Viewer

Length: 1872 bp
ATGAATCTCTCCAATTCTTTTGAGATCGAAGCTACTCAAACACCAGCTTATGTCAGTGGTCAGGCTCAGCTAGCAGAGACCGACGGGGCATTTAGTTCCCGTTCAAATGAGGAAGAAAGGCCAACAATTGGTTCAAGTTTAAGTGGTATCGATACAAGTGTACTGAGTGCTGCAGAAAATGGTGATATTGGTGCCCTTCAGAAACACGGCAAGAATCTTCACCAATTATTGACTGCAACCAAAAACACAGTCCTTCACATTTATATAGCAAGCAGCATAAGCAGCCGAACGTTTTCTAAATCTGATGTAAAGAAGTCAACCGGCATAGTGAAGCAAATCCTTGTTATGTGTCCTTCACTGTTATTGCAGCCCAACGACAACGGTGATATTGCTTTACACGTCGCGGCAAGAGAAGGGCATGCTGCAATAGTTGAAGTTTTTATTGAAGCTGCAAGAGATCGTCGTGAAGACCCTGAGAATGTTGGAGTCGAACAAGTCTGGCAAATGCTTAGGACAACCAACAAGGAGAAGGTCACAGCCATGCATGAAGCCGCGCGCTTTAACCACCTCGGTGTATTGAGAATATTGACGAGAGAAGACCCTGATTTTACATACTCAGCTAACGAAGATGGAGAGACTCCACTTTACCTGGCCGCCGAGAGGGGATATTATCATTTGGTAGTTGAAATATTACAAACTTGCACACATCCAGCTTATGGAGGCCGCTGTGGTAGAACAGCTTTGCACGCTGCAGTGATCTGCAATAGTGAAGGAATGACAAGCAAATTACTAGAGTTCACTAAATGTCCTATGACCAAAGCAGTAGATGAACAAGGATGGACTCCACTTCACTTGGCAGCATTCCTTGGTCACCTTTCAATTGTAGAACAACTACTGGCACATGATAAGTCTGCTGCCTTCATTGGAGACAAGTATGACAATATGACAGCTCTTCACATTGCAGCCTCCAAAGGCCATGTACACGTAATTAAAATGCTCATTTCTCACTGCCCTGATTGTTGTGAACTTGTCGACCGGAGACGCCAGAATGTTCTTCACTATGCGTTCAAAAACAGGTTGTATCAAGTGGAGGACTTAGTTTTGAAGAAGAAATGGCTAAGAAACATCCTCATAAATGGCAAGGATGTTGACGGAAACACTCCTCTCCATCAGCTTGCAGATTCTTTGTATTGGGGTAAAAATTTCGTTGGTGATGCTCAAGTTGATAAACTGGCATTAAACAAGAAAAATCTAAACGCTATCGACATCGTAGTAGCTAAAAATAATACTTTTCCAGTAGACAAGGAAGATTTTAAACTTATGTTGAAAAGAAATGGTGCTAGACCAGGCTATCATCAAATTACAAGGGACGACGAAAATAAAATAGAAGCAGTACAGGAAAAGGCTCACACATTCTCTACAGGCATGGCAAAACAGACCAACCTAGTAGTTGCTGCACTCATAGCAACTGTAACATTTGCAGCAGGTTTCACTGTGCCTGGTGGTTACCAGGGCGAAAAAGGTTTCCCAGTTCTAATAAGAAATTCAGCTTTCAAAGTATTTGTCGCAACAAATACAGCGGCAATGGGACTGTCCACTTCTTCCGTCATGATGTGCCTTTTTTTGTCGGCCGACAAGGAGGAACAAATGACCGAATCTTTTTCATGGTCACTCGTGACGACCTTGCTTGCCTTGTTTGCAATGGTGATTGCATTTCTTAGCGGCACAATTGCAATATTAGGTCATTCTGACCCAGGATTTGCAGGTTTTGTGTGTTACCTTGGCATCGGATACTTCCTTGTGTATGCTATGATCTTCGTCAAGAGGCTCTTTGGTGTTCGTCTAAGACGTCTAACATGCAAGGACGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

623

Amino Acids

68.58

Weight (kDa)

7.08

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 109 - 199 1.7e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 172 - 231 1.3e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 242 - 302 2.1e-10 Ankyrin repeats (3 copies)
Ank PF00023 278 - 301 3.7e-06 Ankyrin repeat
Ank_2 PF12796 310 - 366 8.7e-08 Ankyrin repeats (3 copies)
PGG PF13962 477 - 578 8.6e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1852
Acc36I ACCTGC 2 cut(s) 1475, 1754
AccB1I GGYRCC 1 cut(s) 191
AccB7I CCANNNNNTGG 2 cut(s) 128, 716
AccI GTMKAC 2 cut(s) 1032, 1299
AccII CGCG 3 cut(s) 404, 554, 556
AciI CCGC 6 cut(s) 404, 552, 654, 724, 1580, 1722
AclI AACGTT 1 cut(s) 290
AcoI YGGCCR 2 cut(s) 651, 1629
AcsI RAATTY 2 cut(s) 1201, 1543
AcuI CTGAAG 1 cut(s) 182
AcyI GRCGYC 2 cut(s) 1042, 1849
AfaI GTAC 3 cut(s) 162, 981, 1395
AfiI CCNNNNNNNGG 3 cut(s) 128, 660, 716
AflIII ACRYGT 2 cut(s) 397, 982
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 5 cut(s) 648, 1345, 1498, 1509, 1753
AjuI GAANNNNNNNTTGG 2 cut(s) 115, 147
AleI CACNNNNGTG 1 cut(s) 570
Alw26I GTCTC 4 cut(s) 71, 629, 921, 1033
AoxI GGCC 5 cut(s) 119, 651, 721, 973, 1629
ApoI RAATTY 2 cut(s) 1201, 1543
ArsI GACNNNNNNTTYG 6 cut(s) 234, 266, 1548, 1580, 1742, 1774
Asp700I GAANNNNTTC 1 cut(s) 292
AspLEI GCGC 2 cut(s) 556, 558
AsuHPI GGTGA 6 cut(s) 194, 212, 395, 863, 1223, 1717
AsuNHI GCTAGC 1 cut(s) 70
BaeGI GKGCMC 1 cut(s) 196
BaeI ACNNNNGTAYC 2 cut(s) 144, 177
BanI GGYRCC 1 cut(s) 191
BauI CACGAG 1 cut(s) 1673
BbsI GAAGAC 2 cut(s) 474, 603
BccI CCATC 3 cut(s) 623, 831, 1176
BceAI ACGGC 1 cut(s) 223
BciT130I CCWGG 5 cut(s) 650, 1347, 1500, 1511, 1755
BciVI GTATCC 1 cut(s) 1784
BcoDI GTCTC 4 cut(s) 71, 629, 921, 1033
BfaI CTAG 5 cut(s) 71, 791, 1341, 1445, 1870
BfmI CTRYAG 3 cut(s) 171, 750, 1419
BfuAI ACCTGC 2 cut(s) 1475, 1754
BfuI GTATCC 1 cut(s) 1784
BlpI GCTNAGC 1 cut(s) 66
Bme1390I CCNGG 5 cut(s) 650, 1347, 1500, 1511, 1755
BmgBI CACGTC 1 cut(s) 400
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 5 cut(s) 650, 1347, 1500, 1511, 1755
BmrI ACTGGG 1 cut(s) 1523
BmsI GCATC 2 cut(s) 1204, 1794
BmtI GCTAGC 1 cut(s) 74
BmuI ACTGGG 1 cut(s) 1523
BpiI GAAGAC 2 cut(s) 474, 603
Bpu10I CCTNAGC 1 cut(s) 509
Bpu1102I GCTNAGC 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 1203
Bsa29I ATCGAT 1 cut(s) 150
BsaAI YACGTR 1 cut(s) 985
BsaHI GRCGYC 2 cut(s) 1042, 1849
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 5 cut(s) 568, 865, 1510, 1753, 1780
BsaWI WCCGGW 1 cut(s) 1035
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 660, 716
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 4 cut(s) 900, 1236, 1295, 1529
Bse3DI GCAATG 3 cut(s) 957, 1590, 1707
BseBI CCWGG 5 cut(s) 650, 1347, 1500, 1511, 1755
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 5 cut(s) 568, 865, 1510, 1753, 1780
BseGI GGATG 4 cut(s) 706, 842, 1125, 1150
BseLI CCNNNNNNNGG 3 cut(s) 128, 660, 716
BseMI GCAATG 3 cut(s) 957, 1590, 1707
BseMII CTCAG 4 cut(s) 80, 155, 465, 630
BseNI ACTGG 4 cut(s) 900, 1236, 1295, 1529
BsePI GCGCGC 1 cut(s) 554
BseRI GAGGAG 1 cut(s) 1152
BseSI GKGCMC 1 cut(s) 196
BseX3I CGGCCG 1 cut(s) 1629
BsgI GTGCAG 1 cut(s) 1440
Bsh1236I CGCG 3 cut(s) 404, 554, 556
Bsh1285I CGRYCG 2 cut(s) 1036, 1632
BshFI GGCC 5 cut(s) 121, 653, 723, 975, 1631
BshNI GGYRCC 1 cut(s) 191
BshVI ATCGAT 1 cut(s) 150
BsiEI CGRYCG 2 cut(s) 1036, 1632
BsiSI CCGG 2 cut(s) 321, 1036
BslFI GGGAC 2 cut(s) 1382, 1602
BslI CCNNNNNNNGG 3 cut(s) 128, 660, 716
BsmAI GTCTC 4 cut(s) 71, 629, 921, 1033
BsmBI CGTCTC 1 cut(s) 1033
BsmFI GGGAC 2 cut(s) 1382, 1602
BsmI GAATGC 1 cut(s) 860
BsnI GGCC 5 cut(s) 121, 653, 723, 975, 1631
Bso31I GGTCTC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 196
Bsp1407I TGTACA 1 cut(s) 979
Bsp143I GATC 4 cut(s) 24, 457, 756, 1812
Bsp1720I GCTNAGC 1 cut(s) 66
BspACI CCGC 6 cut(s) 404, 552, 654, 724, 1580, 1722
BspANI GGCC 5 cut(s) 121, 653, 723, 975, 1631
BspCNI CTCAG 4 cut(s) 79, 156, 466, 629
BspDI ATCGAT 1 cut(s) 150
BspFNI CGCG 3 cut(s) 404, 554, 556
BspHI TCATGA 1 cut(s) 1608
BspLI GGNNCC 1 cut(s) 193
BspMAI CTGCAG 2 cut(s) 175, 754
BspMI ACCTGC 2 cut(s) 1475, 1754
BspOI GCTAGC 1 cut(s) 74
BspQI GCTCTTC 1 cut(s) 957
BspT107I GGYRCC 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 71
BsrDI GCAATG 3 cut(s) 957, 1590, 1707
BsrFI RCCGGY 1 cut(s) 320
BsrGI TGTACA 1 cut(s) 979
BsrI ACTGG 4 cut(s) 900, 1236, 1295, 1529
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 5 cut(s) 568, 865, 1510, 1753, 1780
BssHII GCGCGC 1 cut(s) 554
BssMI GATC 4 cut(s) 24, 457, 756, 1812
BssNI GRCGYC 2 cut(s) 1042, 1849
BssSI CACGAG 1 cut(s) 1673
BssT1I CCWWGG 2 cut(s) 865, 1780
Bst2BI CACGAG 1 cut(s) 1673
Bst2UI CCWGG 5 cut(s) 650, 1347, 1500, 1511, 1755
Bst4CI ACNGT 6 cut(s) 250, 360, 383, 1471, 1495, 1593
Bst6I CTCTTC 1 cut(s) 957
BstACI GRCGYC 2 cut(s) 1042, 1849
BstAUI TGTACA 1 cut(s) 979
BstBAI YACGTR 1 cut(s) 985
BstC8I GCNNGC 7 cut(s) 72, 271, 420, 556, 747, 1176, 1689
BstDEI CTNAG 9 cut(s) 66, 164, 474, 509, 616, 1096, 1118, 1718, 1844
BstEII GGTNACC 2 cut(s) 869, 1505
BstF5I GGATG 4 cut(s) 706, 842, 1125, 1150
BstFNI CGCG 3 cut(s) 404, 554, 556
BstHHI GCGC 2 cut(s) 556, 558
BstKTI GATC 4 cut(s) 27, 460, 759, 1815
BstMAI GTCTC 4 cut(s) 71, 629, 921, 1033
BstMBI GATC 4 cut(s) 24, 457, 756, 1812
BstMCI CGRYCG 2 cut(s) 1036, 1632
BstMWI GCNNNNNNNGC 7 cut(s) 71, 279, 282, 746, 1463, 1697, 1866
BstNI CCWGG 5 cut(s) 650, 1347, 1500, 1511, 1755
BstNSI RCATGY 2 cut(s) 422, 1860
BstPI GGTNACC 2 cut(s) 869, 1505
BstSCI CCNGG 5 cut(s) 648, 1345, 1498, 1509, 1753
BstSFI CTRYAG 3 cut(s) 171, 750, 1419
BstSLI GKGCMC 1 cut(s) 196
BstUI CGCG 3 cut(s) 404, 554, 556
BstV2I GAAGAC 2 cut(s) 474, 603
BstZI CGGCCG 1 cut(s) 1629
Bsu15I ATCGAT 1 cut(s) 150
BsuI GTATCC 1 cut(s) 1784
BsuRI GGCC 5 cut(s) 121, 653, 723, 975, 1631
BsuTUI ATCGAT 1 cut(s) 150
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 4 cut(s) 706, 842, 1125, 1150
BtsI GCAGTG 2 cut(s) 759, 1006
BtsIMutI CAGTG 5 cut(s) 61, 356, 759, 1006, 1491
BveI ACCTGC 2 cut(s) 1475, 1754
Cac8I GCNNGC 7 cut(s) 72, 271, 420, 556, 747, 1176, 1689
CciI TCATGA 1 cut(s) 1608
CfoI GCGC 2 cut(s) 556, 558
Cfr10I RCCGGY 1 cut(s) 320
ClaI ATCGAT 1 cut(s) 150
CseI GACGC 1 cut(s) 1050
Csp6I GTAC 3 cut(s) 161, 980, 1394
CspCI CAANNNNNGTGG 2 cut(s) 834, 869
CviAII CATG 9 cut(s) 419, 541, 545, 902, 977, 1426, 1609, 1665, 1857
CviQI GTAC 3 cut(s) 161, 980, 1394
DdeI CTNAG 9 cut(s) 66, 164, 474, 509, 616, 1096, 1118, 1718, 1844
DpnI GATC 4 cut(s) 26, 459, 758, 1814
DpnII GATC 4 cut(s) 24, 457, 756, 1812
DraI TTTAAA 1 cut(s) 1315
EaeI YGGCCR 2 cut(s) 651, 1629
EagI CGGCCG 1 cut(s) 1629
Eam1104I CTCTTC 1 cut(s) 957
EarI CTCTTC 1 cut(s) 957
EclXI CGGCCG 1 cut(s) 1629
Eco130I CCWWGG 2 cut(s) 865, 1780
Eco31I GGTCTC 1 cut(s) 71
Eco52I CGGCCG 1 cut(s) 1629
Eco57I CTGAAG 1 cut(s) 182
Eco91I GGTNACC 2 cut(s) 869, 1505
EcoO65I GGTNACC 2 cut(s) 869, 1505
EcoRII CCWGG 5 cut(s) 648, 1345, 1498, 1509, 1753
EcoT14I CCWWGG 2 cut(s) 865, 1780
EcoT22I ATGCAT 1 cut(s) 546
ErhI CCWWGG 2 cut(s) 865, 1780
Esp3I CGTCTC 1 cut(s) 1033
FaeI CATG 9 cut(s) 422, 544, 548, 905, 980, 1429, 1612, 1668, 1860
FalI AAGNNNNNCTT 2 cut(s) 1814, 1846
FaqI GGGAC 2 cut(s) 1382, 1602
FatI CATG 9 cut(s) 418, 540, 544, 901, 976, 1425, 1608, 1664, 1856
FblI GTMKAC 2 cut(s) 1032, 1299
FokI GGATG 4 cut(s) 693, 849, 1112, 1157
FspBI CTAG 5 cut(s) 71, 791, 1341, 1445, 1870
GlaI GCGC 2 cut(s) 555, 557
HaeIII GGCC 5 cut(s) 121, 653, 723, 975, 1631
HapII CCGG 2 cut(s) 321, 1036
HgaI GACGC 1 cut(s) 1050
HhaI GCGC 2 cut(s) 556, 558
Hin1I GRCGYC 2 cut(s) 1042, 1849
Hin1II CATG 9 cut(s) 422, 544, 548, 905, 980, 1429, 1612, 1668, 1860
Hin6I GCGC 2 cut(s) 554, 556
HinP1I GCGC 2 cut(s) 554, 556
HincII GTYRAC 3 cut(s) 318, 1033, 1150
HindII GTYRAC 3 cut(s) 318, 1033, 1150
HinfI GANTC 7 cut(s) 4, 214, 486, 637, 841, 1181, 1655
HpaII CCGG 2 cut(s) 321, 1036
HphI GGTGA 6 cut(s) 194, 212, 395, 863, 1223, 1717
Hpy166II GTNNAC 9 cut(s) 161, 318, 798, 982, 1025, 1033, 1150, 1300, 1596
Hpy188I TCNGA 4 cut(s) 201, 304, 1750, 1790
Hpy188III TCNNGA 4 cut(s) 464, 1609, 1675, 1822
Hpy8I GTNNAC 9 cut(s) 161, 318, 798, 982, 1025, 1033, 1150, 1300, 1596
Hpy99I CGWCG 4 cut(s) 86, 404, 465, 1376
HpyAV CCTTC 7 cut(s) 206, 263, 363, 407, 523, 764, 928
HpyCH4III ACNGT 6 cut(s) 250, 360, 383, 1471, 1495, 1593
HpyCH4IV ACGT 4 cut(s) 290, 399, 984, 1849
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 279, 282, 746, 1463, 1697, 1866
HpyF3I CTNAG 9 cut(s) 66, 164, 474, 509, 616, 1096, 1118, 1718, 1844
HpySE526I ACGT 4 cut(s) 290, 399, 984, 1849
Hsp92I GRCGYC 2 cut(s) 1042, 1849
Hsp92II CATG 9 cut(s) 422, 544, 548, 905, 980, 1429, 1612, 1668, 1860
HspAI GCGC 2 cut(s) 554, 556
Kzo9I GATC 4 cut(s) 24, 457, 756, 1812
LguI GCTCTTC 1 cut(s) 957
LweI GCATC 2 cut(s) 1204, 1794
MaeI CTAG 5 cut(s) 71, 791, 1341, 1445, 1870
MaeII ACGT 4 cut(s) 290, 399, 984, 1849
MaeIII GTNAC 7 cut(s) 532, 869, 1471, 1505, 1668, 1675, 1775
MalI GATC 4 cut(s) 26, 459, 758, 1814
MboI GATC 4 cut(s) 24, 457, 756, 1812
MfeI CAATTG 3 cut(s) 126, 879, 1728
MhlI GDGCHC 1 cut(s) 196
MlyI GAGTC 3 cut(s) 495, 631, 835
MmeI TCCRAC 1 cut(s) 463
Mph1103I ATGCAT 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 292
MseI TTAA 5 cut(s) 140, 561, 990, 1238, 1314
MslI CAYNNNNRTG 2 cut(s) 539, 570
MspA1I CMGCKG 2 cut(s) 726, 1580
MspI CCGG 2 cut(s) 321, 1036
MspR9I CCNGG 5 cut(s) 650, 1347, 1500, 1511, 1755
MunI CAATTG 3 cut(s) 126, 879, 1728
Mva1269I GAATGC 1 cut(s) 860
MvaI CCWGG 5 cut(s) 650, 1347, 1500, 1511, 1755
MvnI CGCG 3 cut(s) 404, 554, 556
MwoI GCNNNNNNNGC 7 cut(s) 71, 279, 282, 746, 1463, 1697, 1866
NdeII GATC 4 cut(s) 24, 457, 756, 1812
NheI GCTAGC 1 cut(s) 70
NlaIII CATG 9 cut(s) 422, 544, 548, 905, 980, 1429, 1612, 1668, 1860
NlaIV GGNNCC 1 cut(s) 193
NmeAIII GCCGAG 1 cut(s) 682
NmuCI GTSAC 4 cut(s) 532, 869, 1668, 1675
NsiI ATGCAT 1 cut(s) 546
NspI RCATGY 2 cut(s) 422, 1860
OliI CACNNNNGTG 1 cut(s) 570
PaeI GCATGC 1 cut(s) 422
PagI TCATGA 1 cut(s) 1608
PauI GCGCGC 1 cut(s) 554
PciSI GCTCTTC 1 cut(s) 957
PcsI WCGNNNNNNNCGW 1 cut(s) 1846
PctI GAATGC 1 cut(s) 860
PdmI GAANNNNTTC 1 cut(s) 292
PfeI GAWTC 4 cut(s) 4, 214, 1181, 1655
PflMI CCANNNNNTGG 2 cut(s) 128, 716
PleI GAGTC 3 cut(s) 494, 631, 835
PpsI GAGTC 3 cut(s) 494, 631, 835
Ppu21I YACGTR 1 cut(s) 985
Psp1406I AACGTT 1 cut(s) 290
Psp6I CCWGG 5 cut(s) 648, 1345, 1498, 1509, 1753
PspEI GGTNACC 2 cut(s) 869, 1505
PspGI CCWGG 5 cut(s) 648, 1345, 1498, 1509, 1753
PspN4I GGNNCC 1 cut(s) 193
PstI CTGCAG 2 cut(s) 175, 754
PteI GCGCGC 1 cut(s) 554
RsaI GTAC 3 cut(s) 162, 981, 1395
RsaNI GTAC 3 cut(s) 161, 980, 1394
RseI CAYNNNNRTG 2 cut(s) 539, 570
SalI GTCGAC 1 cut(s) 1031
SapI GCTCTTC 1 cut(s) 957
SaqAI TTAA 5 cut(s) 140, 561, 990, 1238, 1314
Sau3AI GATC 4 cut(s) 24, 457, 756, 1812
SchI GAGTC 3 cut(s) 495, 631, 835
ScrFI CCNGG 5 cut(s) 650, 1347, 1500, 1511, 1755
SduI GDGCHC 1 cut(s) 196
SfaNI GCATC 2 cut(s) 1204, 1794
SfcI CTRYAG 3 cut(s) 171, 750, 1419
SmiMI CAYNNNNRTG 2 cut(s) 539, 570
SmlI CTYRAG 1 cut(s) 1218
SmoI CTYRAG 1 cut(s) 1218
SphI GCATGC 1 cut(s) 422
SsiI CCGC 6 cut(s) 404, 552, 654, 724, 1580, 1722
SspI AATATT 3 cut(s) 585, 690, 1737
SspMI CTAG 5 cut(s) 71, 791, 1341, 1445, 1870
StyD4I CCNGG 5 cut(s) 648, 1345, 1498, 1509, 1753
StyI CCWWGG 2 cut(s) 865, 1780
TaaI ACNGT 6 cut(s) 250, 360, 383, 1471, 1495, 1593
TaiI ACGT 4 cut(s) 293, 402, 987, 1852
TaqI TCGA 5 cut(s) 27, 150, 489, 1032, 1263
TaqII GACCGA 2 cut(s) 95, 1667
TatI WGTACW 3 cut(s) 160, 979, 1393
TauI GCSGC 6 cut(s) 407, 554, 656, 726, 1583, 1725
TfiI GAWTC 4 cut(s) 4, 214, 1181, 1655
Tru1I TTAA 5 cut(s) 140, 561, 990, 1238, 1314
Tru9I TTAA 5 cut(s) 140, 561, 990, 1238, 1314
TscAI CASTG 5 cut(s) 61, 363, 759, 1013, 1498
TseFI GTSAC 4 cut(s) 532, 869, 1668, 1675
Tsp45I GTSAC 4 cut(s) 532, 869, 1668, 1675
TspDTI ATGAA 5 cut(s) 17, 561, 843, 910, 1653
TspGWI ACGGA 2 cut(s) 1167, 1594
TspRI CASTG 5 cut(s) 61, 363, 759, 1013, 1498
Van91I CCANNNNNTGG 2 cut(s) 128, 716
XapI RAATTY 2 cut(s) 1201, 1543
XceI RCATGY 2 cut(s) 422, 1860
XmiI GTMKAC 2 cut(s) 1032, 1299
XmnI GAANNNNTTC 1 cut(s) 292
XspI CTAG 5 cut(s) 71, 791, 1341, 1445, 1870
ZraI GACGTC 1 cut(s) 1850
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.