pycom15g20660

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000489
Physical Location & Seq
Reverse (-)
14814734 .. 14815480
747 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g20660.1

Sequence Viewer

Length: 747 bp
ATGGATGTTTCAAATCAGATCACCATCCAGGAAGAAGCAGCTGGTTCAAGTTTTATGGATCTTGACGTGTACAATGCGGCAAAAGCCGGCGATGTCGACGTCCTTCGGAAGCACAGAGACCACCTTGACCGAATGTTGACTCCAACCAAGAACACAATCCTCCATGTTTACATAGCATGCTCAACGTTGACTGAAACTGACTCCGAAGAAGCACTACTACTGGTAACGAAGTCAGCTAATGTCGTCGAGAAGATTGTTGAAATGTGTCCGCCGTTACTGTTCCAGCCCAACGACGGCGGTGACACCGCATTGCACGTTGCGGCGAGGCACGGCTGTACTCGTGTGGTTCAAATTCTTCTTAAGCAGGCTGCGAAAAGAGATTGCGGCCCCGTGGGGAAGCTGATGGGGGCAACCAACAAAGTGAAGGACACGGCGTTGCACGAGGCAGTGCGGTTTAACCACCGTGGTGTGGTGGAGGTGTTGACTAGAGAAGATCCTGATGTTTTGTACTCTGCTAACGTAGATGGCGATACGCCGCTCTACTTGGCTGCTGAGAGGGGATATCGTGATTTGGTTTTCGAAATGCTTGGGACTTGTAAATGTTTAGCTTATGGAGGCCGCAACGGTAGAACGGCTTTGCACGCTGCTGTGATCTGCAACGATGAAGGTACGTATATAATTAATTCACATATTATTTTCATTTACCTTTGGCTTAATTTTTCATTTCTCAAGCTTGAGATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

249

Amino Acids

27.41

Weight (kDa)

5.58

Isoelectric Point (pI)

22.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 26 - 124 3.6e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 89 - 167 5.3e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 136 - 196 4.7e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 102
AccBSI CCGCTC 1 cut(s) 538
AccI GTMKAC 1 cut(s) 96
AciI CCGC 9 cut(s) 77, 269, 297, 306, 320, 384, 451, 536, 619
AclI AACGTT 1 cut(s) 185
AclWI GGATC 2 cut(s) 66, 488
AcsI RAATTY 1 cut(s) 351
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 4 cut(s) 71, 337, 509, 670
AfiI CCNNNNNNNGG 2 cut(s) 293, 469
AflII CTTAAG 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 4 cut(s) 12, 48, 260, 350
AjiI CACGTC 1 cut(s) 67
AjnI CCWGG 1 cut(s) 27
AleI CACNNNNGTG 1 cut(s) 465
AluBI AGCT 5 cut(s) 41, 236, 400, 608, 733
AluI AGCT 5 cut(s) 41, 236, 400, 608, 733
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 2 cut(s) 66, 488
AoxI GGCC 2 cut(s) 385, 616
ApeKI GCWGC 4 cut(s) 38, 368, 548, 644
ApoI RAATTY 1 cut(s) 351
AseI ATTAAT 1 cut(s) 681
AspS9I GGNCC 1 cut(s) 386
AsuHPI GGTGA 2 cut(s) 13, 311
AsuII TTCGAA 1 cut(s) 579
BarI GAAGNNNNNNTAC 4 cut(s) 198, 201, 230, 233
BauI CACGAG 2 cut(s) 339, 440
BbvI GCAGC 4 cut(s) 50, 355, 535, 631
BccI CCATC 3 cut(s) 32, 397, 518
BceAI ACGGC 5 cut(s) 256, 310, 346, 447, 648
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 1 cut(s) 486
BfrI CTTAAG 1 cut(s) 359
BisI GCNGC 9 cut(s) 39, 78, 321, 369, 385, 536, 549, 619, 645
BlsI GCNGC 9 cut(s) 40, 79, 322, 370, 386, 537, 550, 620, 646
Bme1390I CCNGG 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 1 cut(s) 388
BmrFI CCNGG 1 cut(s) 29
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 713
Bsa29I ATCGAT 1 cut(s) 741
BsaAI YACGTR 1 cut(s) 672
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 390, 463
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 469
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 308
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 1 cut(s) 29
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 390, 463
BseGI GGATG 2 cut(s) 10, 24
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 2 cut(s) 293, 469
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 1 cut(s) 543
BseNI ACTGG 1 cut(s) 225
BseXI GCAGC 4 cut(s) 50, 355, 535, 631
BshFI GGCC 2 cut(s) 387, 618
BshVI ATCGAT 1 cut(s) 741
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 2 cut(s) 293, 469
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 604
BsnI GGCC 2 cut(s) 387, 618
Bso31I GGTCTC 1 cut(s) 111
Bsp119I TTCGAA 1 cut(s) 579
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 5 cut(s) 18, 58, 493, 651, 738
BspACI CCGC 9 cut(s) 77, 269, 297, 306, 320, 384, 451, 536, 619
BspANI GGCC 2 cut(s) 387, 618
BspCNI CTCAG 1 cut(s) 544
BspDI ATCGAT 1 cut(s) 741
BspLI GGNNCC 1 cut(s) 388
BspPI GGATC 2 cut(s) 66, 488
BspT104I TTCGAA 1 cut(s) 579
BspTI CTTAAG 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 111
BsrBI CCGCTC 1 cut(s) 538
BsrDI GCAATG 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 86
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 1 cut(s) 225
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 2 cut(s) 390, 463
BssMI GATC 5 cut(s) 18, 58, 493, 651, 738
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 2 cut(s) 339, 440
Bst2BI CACGAG 2 cut(s) 339, 440
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 3 cut(s) 279, 464, 626
BstACI GRCGYC 1 cut(s) 99
BstAFI CTTAAG 1 cut(s) 359
BstAUI TGTACA 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 672
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 4 cut(s) 88, 178, 366, 642
BstDEI CTNAG 1 cut(s) 552
BstDSI CCRYGG 2 cut(s) 390, 463
BstF5I GGATG 2 cut(s) 10, 24
BstKTI GATC 5 cut(s) 21, 61, 496, 654, 741
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 5 cut(s) 18, 58, 493, 651, 738
BstMWI GCNNNNNNNGC 2 cut(s) 83, 641
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 180
BstSCI CCNGG 1 cut(s) 27
BstSNI TACGTA 1 cut(s) 672
BstV1I GCAGC 4 cut(s) 50, 355, 535, 631
BstX2I RGATCY 2 cut(s) 58, 493
BstYI RGATCY 2 cut(s) 58, 493
Bsu15I ATCGAT 1 cut(s) 741
BsuRI GGCC 2 cut(s) 387, 618
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 2 cut(s) 390, 463
BtgZI GCGATG 1 cut(s) 105
BtrI CACGTC 1 cut(s) 67
BtsCI GGATG 2 cut(s) 10, 24
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 453
Cac8I GCNNGC 4 cut(s) 88, 178, 366, 642
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 386
ClaI ATCGAT 1 cut(s) 741
Csp6I GTAC 4 cut(s) 70, 336, 508, 669
CviAII CATG 2 cut(s) 164, 177
CviQI GTAC 4 cut(s) 70, 336, 508, 669
DdeI CTNAG 1 cut(s) 552
DpnI GATC 5 cut(s) 20, 60, 495, 653, 740
DpnII GATC 5 cut(s) 18, 58, 493, 651, 738
EciI GGCGGA 1 cut(s) 258
Eco105I TACGTA 1 cut(s) 672
Eco31I GGTCTC 1 cut(s) 111
Eco32I GATATC 1 cut(s) 563
EcoRII CCWGG 1 cut(s) 27
EcoRV GATATC 1 cut(s) 563
FaeI CATG 2 cut(s) 167, 180
FaiI YATR 8 cut(s) 56, 165, 173, 178, 612, 675, 677, 690
FaqI GGGAC 1 cut(s) 604
FatI CATG 2 cut(s) 163, 176
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 9 cut(s) 39, 78, 321, 369, 385, 536, 549, 619, 645
FokI GGATG 2 cut(s) 11, 17
Fsp4HI GCNGC 9 cut(s) 39, 78, 321, 369, 385, 536, 549, 619, 645
FspBI CTAG 1 cut(s) 486
GluI GCNGC 9 cut(s) 39, 78, 321, 369, 385, 536, 549, 619, 645
HaeIII GGCC 2 cut(s) 387, 618
HapII CCGG 1 cut(s) 87
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 2 cut(s) 167, 180
HincII GTYRAC 4 cut(s) 97, 138, 189, 483
HindII GTYRAC 4 cut(s) 97, 138, 189, 483
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 2 cut(s) 139, 200
HpaII CCGG 1 cut(s) 87
HphI GGTGA 2 cut(s) 13, 311
Hpy166II GTNNAC 6 cut(s) 70, 97, 138, 169, 189, 483
Hpy188I TCNGA 3 cut(s) 18, 108, 205
Hpy188III TCNNGA 4 cut(s) 62, 247, 497, 566
Hpy8I GTNNAC 6 cut(s) 70, 97, 138, 169, 189, 483
Hpy99I CGWCG 3 cut(s) 101, 248, 296
HpyAV CCTTC 3 cut(s) 113, 418, 659
HpyCH4III ACNGT 3 cut(s) 279, 464, 626
HpyCH4IV ACGT 6 cut(s) 66, 99, 185, 315, 519, 671
HpyCH4V TGCA 4 cut(s) 313, 439, 640, 657
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 641
HpyF3I CTNAG 1 cut(s) 552
HpySE526I ACGT 6 cut(s) 66, 99, 185, 315, 519, 671
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 2 cut(s) 167, 180
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 5 cut(s) 18, 58, 493, 651, 738
LpnPI CCDG 8 cut(s) 14, 27, 41, 100, 206, 296, 350, 510
Lsp1109I GCAGC 4 cut(s) 50, 355, 535, 631
MaeI CTAG 1 cut(s) 486
MaeII ACGT 6 cut(s) 66, 99, 185, 315, 519, 671
MaeIII GTNAC 3 cut(s) 223, 273, 299
MalI GATC 5 cut(s) 20, 60, 495, 653, 740
MbiI CCGCTC 1 cut(s) 538
MboI GATC 5 cut(s) 18, 58, 493, 651, 738
MboII GAAGA 5 cut(s) 44, 218, 262, 347, 503
MflI RGATCY 2 cut(s) 58, 493
MluCI AATT 4 cut(s) 351, 678, 682, 715
MlyI GAGTC 2 cut(s) 133, 194
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 6 cut(s) 170, 318, 436, 469, 549, 608
MroNI GCCGGC 1 cut(s) 86
MseI TTAA 4 cut(s) 360, 456, 681, 714
MslI CAYNNNNRTG 1 cut(s) 465
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MwoI GCNNNNNNNGC 2 cut(s) 83, 641
NaeI GCCGGC 1 cut(s) 88
NdeII GATC 5 cut(s) 18, 58, 493, 651, 738
NgoMIV GCCGGC 1 cut(s) 86
NlaIII CATG 2 cut(s) 167, 180
NlaIV GGNNCC 1 cut(s) 388
NmuCI GTSAC 1 cut(s) 299
NspI RCATGY 1 cut(s) 180
NspV TTCGAA 1 cut(s) 579
OliI CACNNNNGTG 1 cut(s) 465
PaeI GCATGC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 525
PdiI GCCGGC 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 9 cut(s) 40, 79, 322, 370, 386, 537, 550, 620, 646
PleI GAGTC 2 cut(s) 133, 194
PpsI GAGTC 2 cut(s) 133, 194
Ppu21I YACGTR 1 cut(s) 672
PshBI ATTAAT 1 cut(s) 681
Psp1406I AACGTT 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 388
PspPI GGNCC 1 cut(s) 386
PsuI RGATCY 2 cut(s) 58, 493
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 4 cut(s) 71, 337, 509, 670
RsaNI GTAC 4 cut(s) 70, 336, 508, 669
RseI CAYNNNNRTG 1 cut(s) 465
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 4 cut(s) 360, 456, 681, 714
SatI GCNGC 9 cut(s) 39, 78, 321, 369, 385, 536, 549, 619, 645
Sau3AI GATC 5 cut(s) 18, 58, 493, 651, 738
Sau96I GGNCC 1 cut(s) 386
SchI GAGTC 2 cut(s) 133, 194
ScrFI CCNGG 1 cut(s) 29
SfuI TTCGAA 1 cut(s) 579
SmiMI CAYNNNNRTG 1 cut(s) 465
SmlI CTYRAG 3 cut(s) 359, 728, 734
SmoI CTYRAG 3 cut(s) 359, 728, 734
SnaBI TACGTA 1 cut(s) 672
SphI GCATGC 1 cut(s) 180
Sse9I AATT 4 cut(s) 351, 678, 682, 715
SsiI CCGC 9 cut(s) 77, 269, 297, 306, 320, 384, 451, 536, 619
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 27
TaaI ACNGT 3 cut(s) 279, 464, 626
TaiI ACGT 6 cut(s) 69, 102, 188, 318, 522, 674
TaqI TCGA 4 cut(s) 96, 246, 579, 741
TaqII GACCGA 1 cut(s) 144
TasI AATT 4 cut(s) 351, 678, 682, 715
TatI WGTACW 3 cut(s) 69, 335, 507
TauI GCSGC 5 cut(s) 80, 323, 387, 538, 621
Tru1I TTAA 4 cut(s) 360, 456, 681, 714
Tru9I TTAA 4 cut(s) 360, 456, 681, 714
TscAI CASTG 1 cut(s) 453
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 4 cut(s) 38, 368, 548, 644
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 3 cut(s) 678, 688, 711
TspRI CASTG 1 cut(s) 453
Vha464I CTTAAG 1 cut(s) 359
VspI ATTAAT 1 cut(s) 681
XapI RAATTY 1 cut(s) 351
XceI RCATGY 1 cut(s) 180
XmiI GTMKAC 1 cut(s) 96
XspI CTAG 1 cut(s) 486
ZraI GACGTC 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.