MD15G1232100.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000489
Physical Location & Seq
Reverse (-)
18952362 .. 18955044
2683 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1232100.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGGATCATTCCCATTCAACTGATCAGGCCGAGAGTGCTAGTAGTACTGACCACTTAGCAGTTCAGACTAAGGAGGGAGCTACTAAAGAAGCCAATAATACAGTTTCGATAGGTGGCATGGAACTGGATGTGTTTCGTGCTGCGAAACAAGGCGACCTTGATGCCCTCAGGGAACATGCAGAACATCTTGACCTAAAACTGACTGCAACAAATAACACGGTCCTTCTTGTTTATATAGCGTGCTTAAGCAGGCCGACATCTGGACAAAACACATTGGAGTCAGCCGGGATTGTGAAGGATATCCTTGAAATGTGTCCCGCACTACTATGGCAGCACAATAAGAGCGGTGAGACTGCCTTACACCTAGCTGCAAGACAGGGGCTAATTGAAATAGTTGGAGTTCTTATCGATCAGGCAACAAAAGTTCGTCACCATGGCGACCTTGAGCAAGGCCGACTCTTAACAGAAGAAGAAGCCTGCTTGCAGAAGCAAATGCTCATTAGGAAAACCAACAACAACAAGAATACAGCGTTGCACGAAGCAGTGCGCTTTAATCAATGTTTTGTGGTGGAGAAATTGACCATAGAAGACCCCGAGTTTTCCTACGCTGCTAATGATTCAGGTGAAACTCCAATTTATTTAGCTGCCGAGAGGGGATATCTTCCTTTGGTTTCTAAAATGCTCAAGAATTGCAGTGTTCCAACTTACACTGGCCCAAATGGTAGAAGGGCGTTGCACGCTGCAGTGATTCAAATGGATAAAGAAATGACGGAGGAACTGCTAAAAGCCGATAGGACTTTGAGCAAAGCAGCAGACGAACTAGGATGTACTCCACTACACCTTGCTGCACGTATTGGTTCCGTTTCAATTGTTAAACAACTGCTTGCAAATGATAGATCTACTGCTTACATTGCTGACAAAGATGGCGCGACAGCTCTTCACTTTGCAGCGTCCGAAGGCAATCTAGATGTAATGAAAGAGCTTATTTCTCAATGTCCTGATTGTTGCGAACTGGTCGACAACAAATTTCAGAATGTTCTTTACTACGCCATACAGAACAAACAAAACCAAATAGTAAAATATGTTTTAGGAGATGCATGGCTTAGAAACATCCTGTTAAATGGCATGGATGTTGATGGGAACACACCCCTCCATCACCTAGCTACTCATTCTCCCAAAAGCATAGACTTACTAGATGGTCATAACGTTGATTGGATGGCATTCAACAAGCAAAACTTAAACGCTTTTGAAATAGTTCTAGCTAACGATGATGATTGTAGACATCGAAATTTCGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

434

Amino Acids

47.77

Weight (kDa)

5.56

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 97 - 194 1.3e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 203 - 262 9.3e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 270 - 331 4.6e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 305 - 363 2.6e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 345
AccI GTMKAC 2 cut(s) 1017, 1279
AccII CGCG 1 cut(s) 929
AciI CCGC 2 cut(s) 318, 345
AclI AACGTT 1 cut(s) 1206
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 1025, 1288
AfaI GTAC 2 cut(s) 46, 829
AfiI CCNNNNNNNGG 1 cut(s) 260
AflII CTTAAG 1 cut(s) 244
AgsI TTSAA 7 cut(s) 18, 308, 389, 752, 867, 1225, 1250
AluBI AGCT 7 cut(s) 80, 368, 644, 935, 982, 1163, 1262
AluI AGCT 7 cut(s) 80, 368, 644, 935, 982, 1163, 1262
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 593
AoxI GGCC 4 cut(s) 27, 251, 451, 712
ApeKI GCWGC 9 cut(s) 140, 331, 368, 608, 644, 740, 809, 845, 947
ApoI RAATTY 2 cut(s) 1025, 1288
Asp700I GAANNNNTTC 1 cut(s) 1254
AspLEI GCGC 2 cut(s) 549, 929
AspS9I GGNCC 2 cut(s) 220, 713
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 4 cut(s) 359, 422, 635, 1148
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 1 cut(s) 220
AxyI CCTNAGG 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 594
BbvI GCAGC 9 cut(s) 127, 343, 355, 595, 631, 727, 821, 832, 959
BccI CCATC 5 cut(s) 917, 1130, 1161, 1190, 1210
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BclI TGATCA 1 cut(s) 22
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 7 cut(s) 39, 365, 821, 965, 1160, 1193, 1259
BfmI CTRYAG 1 cut(s) 741
BfrI CTTAAG 1 cut(s) 244
BglII AGATCT 1 cut(s) 896
BisI GCNGC 9 cut(s) 141, 332, 369, 609, 645, 741, 810, 846, 948
BlsI GCNGC 9 cut(s) 142, 333, 370, 610, 646, 742, 811, 847, 949
BmcAI AGTACT 1 cut(s) 46
Bme1390I CCNGG 1 cut(s) 286
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 2 cut(s) 220, 713
BmiI GGNNCC 1 cut(s) 859
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 2 cut(s) 151, 1084
BpiI GAAGAC 1 cut(s) 594
BpuEI CTTGAG 2 cut(s) 464, 668
BpuMI CCSGG 1 cut(s) 286
Bsa29I ATCGAT 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 851
BsaJI CCNNGG 1 cut(s) 433
BsaXI ACNNNNNCTCC 2 cut(s) 1156, 1186
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 3 cut(s) 129, 715, 1017
Bse21I CCTNAGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 909
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 5 cut(s) 133, 830, 1110, 1135, 1221
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 1 cut(s) 909
BseMII CTCAG 1 cut(s) 181
BseNI ACTGG 3 cut(s) 129, 715, 1017
BseXI GCAGC 9 cut(s) 127, 343, 355, 595, 631, 727, 821, 832, 959
BsgI GTGCAG 1 cut(s) 831
Bsh1236I CGCG 1 cut(s) 929
BshFI GGCC 4 cut(s) 29, 253, 453, 714
BshVI ATCGAT 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 1 cut(s) 285
BslFI GGGAC 1 cut(s) 300
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 300
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 4 cut(s) 29, 253, 453, 714
BsoBI CYCGRG 1 cut(s) 593
Bsp143I GATC 4 cut(s) 4, 22, 409, 896
Bsp19I CCATGG 1 cut(s) 433
BspACI CCGC 2 cut(s) 318, 345
BspANI GGCC 4 cut(s) 29, 253, 453, 714
BspCNI CTCAG 1 cut(s) 180
BspDI ATCGAT 1 cut(s) 408
BspFNI CGCG 1 cut(s) 929
BspLI GGNNCC 1 cut(s) 859
BspMAI CTGCAG 1 cut(s) 745
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 942
BspTI CTTAAG 1 cut(s) 244
BsrBI CCGCTC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 909
BsrI ACTGG 3 cut(s) 129, 715, 1017
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 4 cut(s) 4, 22, 409, 896
BssT1I CCWWGG 1 cut(s) 433
Bst4CI ACNGT 2 cut(s) 103, 220
Bst6I CTCTTC 1 cut(s) 942
BstAFI CTTAAG 1 cut(s) 244
BstBAI YACGTR 1 cut(s) 851
BstC8I GCNNGC 6 cut(s) 241, 251, 478, 482, 738, 885
BstDEI CTNAG 4 cut(s) 55, 69, 167, 1103
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 5 cut(s) 133, 830, 1110, 1135, 1221
BstFNI CGCG 1 cut(s) 929
BstHHI GCGC 2 cut(s) 549, 929
BstKTI GATC 4 cut(s) 7, 25, 412, 899
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 4 cut(s) 4, 22, 409, 896
BstMWI GCNNNNNNNGC 3 cut(s) 35, 737, 911
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 284
BstSFI CTRYAG 1 cut(s) 741
BstUI CGCG 1 cut(s) 929
BstV1I GCAGC 9 cut(s) 127, 343, 355, 595, 631, 727, 821, 832, 959
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 1 cut(s) 896
BstYI RGATCY 1 cut(s) 896
Bsu15I ATCGAT 1 cut(s) 408
Bsu36I CCTNAGG 1 cut(s) 167
BsuRI GGCC 4 cut(s) 29, 253, 453, 714
BsuTUI ATCGAT 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 433
BtsCI GGATG 5 cut(s) 133, 830, 1110, 1135, 1221
BtsI GCAGTG 3 cut(s) 549, 700, 750
BtsIMutI CAGTG 4 cut(s) 549, 700, 708, 750
Cac8I GCNNGC 6 cut(s) 241, 251, 478, 482, 738, 885
CfoI GCGC 2 cut(s) 549, 929
Cfr13I GGNCC 2 cut(s) 220, 713
ClaI ATCGAT 1 cut(s) 408
CseI GACGC 1 cut(s) 939
Csp6I GTAC 2 cut(s) 45, 828
CviAII CATG 5 cut(s) 118, 176, 434, 1098, 1126
CviQI GTAC 2 cut(s) 45, 828
DdeI CTNAG 4 cut(s) 55, 69, 167, 1103
DpnI GATC 4 cut(s) 6, 24, 411, 898
DpnII GATC 4 cut(s) 4, 22, 409, 896
Eam1104I CTCTTC 1 cut(s) 942
EarI CTCTTC 1 cut(s) 942
Eco130I CCWWGG 1 cut(s) 433
Eco32I GATATC 2 cut(s) 301, 659
Eco47I GGWCC 1 cut(s) 220
Eco81I CCTNAGG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 593
EcoRV GATATC 2 cut(s) 301, 659
EcoT14I CCWWGG 1 cut(s) 433
EcoT22I ATGCAT 1 cut(s) 1099
ErhI CCWWGG 1 cut(s) 433
FaeI CATG 5 cut(s) 121, 179, 437, 1101, 1129
FalI AAGNNNNNCTT 4 cut(s) 141, 173, 1220, 1252
FaqI GGGAC 1 cut(s) 300
FatI CATG 5 cut(s) 117, 175, 433, 1097, 1125
FauI CCCGC 1 cut(s) 325
FbaI TGATCA 1 cut(s) 22
FblI GTMKAC 2 cut(s) 1017, 1279
Fnu4HI GCNGC 9 cut(s) 141, 332, 369, 609, 645, 741, 810, 846, 948
FokI GGATG 5 cut(s) 140, 837, 1097, 1142, 1228
Fsp4HI GCNGC 9 cut(s) 141, 332, 369, 609, 645, 741, 810, 846, 948
FspBI CTAG 7 cut(s) 39, 365, 821, 965, 1160, 1193, 1259
GlaI GCGC 2 cut(s) 548, 928
GluI GCNGC 9 cut(s) 141, 332, 369, 609, 645, 741, 810, 846, 948
HaeIII GGCC 4 cut(s) 29, 253, 453, 714
HapII CCGG 1 cut(s) 285
HgaI GACGC 1 cut(s) 939
HhaI GCGC 2 cut(s) 549, 929
Hin1II CATG 5 cut(s) 121, 179, 437, 1101, 1129
Hin6I GCGC 2 cut(s) 547, 927
HinP1I GCGC 2 cut(s) 547, 927
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HinfI GANTC 4 cut(s) 278, 456, 617, 748
HpaII CCGG 1 cut(s) 285
HphI GGTGA 4 cut(s) 359, 422, 635, 1148
Hpy166II GTNNAC 2 cut(s) 1018, 1280
Hpy188I TCNGA 3 cut(s) 66, 955, 1032
Hpy188III TCNNGA 5 cut(s) 188, 261, 685, 965, 998
Hpy8I GTNNAC 2 cut(s) 1018, 1280
HpyAV CCTTC 4 cut(s) 233, 289, 720, 950
HpyCH4III ACNGT 2 cut(s) 103, 220
HpyCH4IV ACGT 2 cut(s) 850, 1206
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 737, 911
HpyF3I CTNAG 4 cut(s) 55, 69, 167, 1103
HpySE526I ACGT 2 cut(s) 850, 1206
Hsp92II CATG 5 cut(s) 121, 179, 437, 1101, 1129
HspAI GCGC 2 cut(s) 547, 927
Ksp22I TGATCA 1 cut(s) 22
Kzo9I GATC 4 cut(s) 4, 22, 409, 896
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 1 cut(s) 77
Lsp1109I GCAGC 9 cut(s) 127, 343, 355, 595, 631, 727, 821, 832, 959
LweI GCATC 2 cut(s) 151, 1084
MaeI CTAG 7 cut(s) 39, 365, 821, 965, 1160, 1193, 1259
MaeII ACGT 2 cut(s) 850, 1206
MaeIII GTNAC 1 cut(s) 428
MalI GATC 4 cut(s) 6, 24, 411, 898
MbiI CCGCTC 1 cut(s) 345
MboI GATC 4 cut(s) 4, 22, 409, 896
MboII GAAGA 5 cut(s) 479, 482, 599, 653, 929
MfeI CAATTG 1 cut(s) 867
MflI RGATCY 1 cut(s) 896
MluCI AATT 7 cut(s) 384, 575, 633, 688, 867, 1025, 1288
MlyI GAGTC 2 cut(s) 287, 450
MmeI TCCRAC 2 cut(s) 376, 725
MnlI CCTC 5 cut(s) 67, 176, 645, 766, 1160
Mph1103I ATGCAT 1 cut(s) 1099
MroXI GAANNNNTTC 1 cut(s) 1254
MseI TTAA 6 cut(s) 245, 461, 552, 873, 1118, 1238
MslI CAYNNNNRTG 1 cut(s) 325
MspCI CTTAAG 1 cut(s) 244
MspI CCGG 1 cut(s) 285
MspR9I CCNGG 1 cut(s) 286
MunI CAATTG 1 cut(s) 867
Mva1269I GAATGC 1 cut(s) 1220
MvnI CGCG 1 cut(s) 929
MwoI GCNNNNNNNGC 3 cut(s) 35, 737, 911
NciI CCSGG 1 cut(s) 286
NcoI CCATGG 1 cut(s) 433
NdeII GATC 4 cut(s) 4, 22, 409, 896
NlaIII CATG 5 cut(s) 121, 179, 437, 1101, 1129
NlaIV GGNNCC 1 cut(s) 859
NmeAIII GCCGAG 2 cut(s) 55, 673
NmuCI GTSAC 1 cut(s) 428
NsiI ATGCAT 1 cut(s) 1099
NspI RCATGY 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 942
PctI GAATGC 1 cut(s) 1220
PdmI GAANNNNTTC 1 cut(s) 1254
PfeI GAWTC 2 cut(s) 617, 748
PkrI GCNGC 9 cut(s) 142, 333, 370, 610, 646, 742, 811, 847, 949
PleI GAGTC 2 cut(s) 286, 450
PpsI GAGTC 2 cut(s) 286, 450
Ppu21I YACGTR 1 cut(s) 851
Psp1406I AACGTT 1 cut(s) 1206
PspN4I GGNNCC 1 cut(s) 859
PspPI GGNCC 2 cut(s) 220, 713
PstI CTGCAG 1 cut(s) 745
PsuI RGATCY 1 cut(s) 896
RsaI GTAC 2 cut(s) 46, 829
RsaNI GTAC 2 cut(s) 45, 828
RseI CAYNNNNRTG 1 cut(s) 325
SalI GTCGAC 1 cut(s) 1016
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 6 cut(s) 245, 461, 552, 873, 1118, 1238
SatI GCNGC 9 cut(s) 141, 332, 369, 609, 645, 741, 810, 846, 948
Sau3AI GATC 4 cut(s) 4, 22, 409, 896
Sau96I GGNCC 2 cut(s) 220, 713
ScaI AGTACT 1 cut(s) 46
SchI GAGTC 2 cut(s) 287, 450
ScrFI CCNGG 1 cut(s) 286
SfaNI GCATC 2 cut(s) 151, 1084
SfcI CTRYAG 1 cut(s) 741
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 325
SmlI CTYRAG 3 cut(s) 244, 443, 683
SmoI CTYRAG 3 cut(s) 244, 443, 683
Sse9I AATT 7 cut(s) 384, 575, 633, 688, 867, 1025, 1288
SsiI CCGC 2 cut(s) 318, 345
SspMI CTAG 7 cut(s) 39, 365, 821, 965, 1160, 1193, 1259
StyD4I CCNGG 1 cut(s) 284
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 2 cut(s) 103, 220
TaiI ACGT 2 cut(s) 853, 1209
TaqI TCGA 4 cut(s) 107, 408, 1017, 1285
TasI AATT 7 cut(s) 384, 575, 633, 688, 867, 1025, 1288
TatI WGTACW 2 cut(s) 44, 827
TfiI GAWTC 2 cut(s) 617, 748
Tru1I TTAA 6 cut(s) 245, 461, 552, 873, 1118, 1238
Tru9I TTAA 6 cut(s) 245, 461, 552, 873, 1118, 1238
TscAI CASTG 4 cut(s) 549, 700, 715, 750
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 9 cut(s) 140, 331, 368, 608, 644, 740, 809, 845, 947
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 1 cut(s) 989
TspGWI ACGGA 2 cut(s) 785, 850
TspRI CASTG 4 cut(s) 549, 700, 715, 750
Vha464I CTTAAG 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 2 cut(s) 1025, 1288
XbaI TCTAGA 1 cut(s) 964
XceI RCATGY 1 cut(s) 179
XmiI GTMKAC 2 cut(s) 1017, 1279
XmnI GAANNNNTTC 1 cut(s) 1254
XspI CTAG 7 cut(s) 39, 365, 821, 965, 1160, 1193, 1259
ZrmI AGTACT 1 cut(s) 46
Zsp2I ATGCAT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.