Rroxscaffold_2G00144850

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000489
Physical Location & Seq
Forward (+)
82753886 .. 82758049
4164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144850.1

Sequence Viewer

Length: 2028 bp
ATGGACCAACCTTCCACTTCTTCTGACAATGAGAGTGGTGAAGCAACACAGCTCGAGATTGAGAAGGATCGGGCAATAGCAGCTAATGCCGAATCCAGCCAAGAAGTTGAAGCACAAGATGAATGCTTAACTGGCATGGATCTTCCAGTATACAGGGCCGCAACAACAGGCAATATTGATTTCCTTGTGTTACACGGAGAGCATCTATCCCAAATATTGACTCCAACAAAGAACACAGTTCTCCATATTTATATTGCATGCGCAAAGAGAAGCCGACCGTCATCGGAAAACACAGCCAGCATCAATACGGTTACAAGAATCCTCGACATGTGTCCAGCACTGCTATTGCAAGCCAACGAGAGAGGTGAAACAACCTTACATGTGGCCGCGAAACACGGGCATGATCACATAGTTGAACATCTTATCAAGATGGCTAGAGCTCGTCAGGAGGAAGCTTGTTGCCAGCTGCTCATCAGGACAGTCAATCATGACGGAGACACGGCCTTGCACGAAGCAGTGCGCTTCAATCACCTCACTGTGGTGGAGATATTGACCAGAGCAGACCCTGAGGCTTTGTACTCTGCTAATGAAGCAGGAGAGACCCCACTCTACATGGCTGCTCAAAGAGGTTATAATTCTTTGGTTTCTGAAATACTTGAGACTTGCACAAATCCAAGTTATGAAGGTCCTGATGGTAGAACTGCATTGCACGCTTCGGTGATCTACAACGATCGGGATCTCATGAGCAAATTACTAGACTACTTCACTGGTGATAAAGCATTGACAAAAGCAGTTGATAAACAAGGATGGACTCCACTTCACTTTGCTGCATTTTTCGGCCACTCTTTAATTGTAAAGCACTTGCTAAGAGCTGATGAGTCTGCTGCTTATGTTCGTGACAAAGATGATAAGAAGACGGCCCTTCACATTGCTGTCTCCAAAGGTCATCTAGATGTAATGAAAGAGCTGATTTCTCATTGCCCTGATTGTTGCGAGCTGGTCGACAAGAGGCATCAAAATATTCTTCACCATGCAATTGAAAATAAAAGATTTGAAGTACAAGATTTTGTTCAGAGAGACCCCTGGCTTAGCAATGTTCTCTTAAATGGCAAAGATGATAACCAAAATACACCTTTTCATCAAATTGCTGCTTCTTTCTACAAATTCCGTCACGTGAGTCGGGCTGGTTCTGAAATTATTGACTCGAGCATGGATTTCATAAGTGACGCTAGAGTTGATAAGATGGCATTCAATAAGGAACACCTGACTGCTTTGGATATGATTCAAGCTAACCATAGTTTTTCGCGACTAGAGAAGAAATATGTGCAAAGACTCTCTTCGAAAGTGAACAGTGGCAGACCAGGTTATCACACTGTAAAAGACGAAATCAAAGGAAATAAAGACAAAGAAATCAAAATTGAGAGGAAGGAAGCAACTGATCAAATAGTGAAGGAATTAAAACAATCCCATCTCATAGCAGCTACACTCATAGCAACTGTAACCTTCACAGCAGGTATGACCATGCCAGGCGGTTATCAAAGTCAAAAAGGACGGGACCAGGGTTTGGCAGTTCTAACCAAAGATGCCAGTTTCAATGTATTCATTGTGACAAATTTAATTGCCATGACTTTATCCAGTTTGGCTGTCTTCATCCACATTTGGGTCCTTCTCACTGCTAGGCAGTATACACCATTCGTACAGATTCGCTGGGTATTACACTTGACCTTATGGGCATTGGTATGCATGATGGTTGCTTTCTATTACGGCACACTTGCAGTATTGAGGGATTCTTTAGTAGCTAACATTGCCATCAATATCATGTACAACATGCTCGTATTAGTTATCATGCCTTTTGCACTTTTCCTCAATATTTCCACCCTTAATAGTACCGATGCCTTTTATATGTTTCTGGTCAATATACGGGTGCCTACTGCATTCCGTTTTTGCATGTTTCATCTTAGGATGGAAGGCCGCGATGATGGTGGGGCGGGCTTGCGGTGGCAGCGGCAATGTGGAGGTCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

675

Amino Acids

75.82

Weight (kDa)

6.44

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 113 - 188 1.2e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 151 - 220 2.7e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 196 - 254 3.4e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 203 - 290 1.9e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 284 - 353 7.8e-10 Ankyrin repeats (3 copies)
PGG PF13962 483 - 592 1.6e-27 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
Acc16I TGCGCA 1 cut(s) 262
Acc36I ACCTGC 1 cut(s) 1502
AccB1I GGYRCC 1 cut(s) 1924
AccB7I CCANNNNNTGG 1 cut(s) 1564
AccI GTMKAC 3 cut(s) 150, 1002, 1685
AccII CGCG 3 cut(s) 389, 1306, 1974
AciI CCGC 7 cut(s) 159, 387, 1530, 1972, 1988, 1996, 2005
AclWI GGATC 3 cut(s) 75, 147, 744
AcoI YGGCCR 2 cut(s) 384, 838
AcsI RAATTY 2 cut(s) 1163, 1612
AcvI CACGTG 1 cut(s) 1174
AfaI GTAC 5 cut(s) 578, 1059, 1698, 1823, 1888
AfiI CCNNNNNNNGG 3 cut(s) 538, 1564, 1660
AflIII ACRYGT 2 cut(s) 327, 379
AgsI TTSAA 8 cut(s) 110, 416, 526, 1040, 1055, 1252, 1286, 1594
AjnI CCWGG 4 cut(s) 1082, 1360, 1525, 1557
AleI CACNNNNGTG 1 cut(s) 539
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 5 cut(s) 489, 593, 653, 940, 1071
AlwI GGATC 3 cut(s) 75, 147, 744
AlwNI CAGNNNCTG 1 cut(s) 566
Ama87I CYCGRG 2 cut(s) 53, 1204
AoxI GGCC 6 cut(s) 156, 384, 501, 838, 918, 1969
ApeKI GCWGC 8 cut(s) 80, 466, 617, 827, 884, 1148, 1478, 2002
ApoI RAATTY 2 cut(s) 1163, 1612
AspLEI GCGC 2 cut(s) 263, 522
AspS9I GGNCC 6 cut(s) 4, 156, 686, 919, 1555, 1663
AsuHPI GGTGA 6 cut(s) 50, 377, 521, 730, 782, 1019
AsuII TTCGAA 1 cut(s) 1340
AvaI CYCGRG 2 cut(s) 53, 1204
AvaII GGWCC 4 cut(s) 4, 686, 1555, 1663
AxyI CCTNAGG 1 cut(s) 567
BanI GGYRCC 1 cut(s) 1924
BanII GRGCYC 1 cut(s) 442
BbrPI CACGTG 1 cut(s) 1174
BbsI GAAGAC 2 cut(s) 920, 1639
Bbv12I GWGCWC 1 cut(s) 442
BbvI GCAGC 8 cut(s) 92, 453, 604, 814, 871, 1135, 1490, 2014
BccI CCATC 9 cut(s) 424, 686, 801, 1237, 1476, 1741, 1817, 1957, 1973
BceAI ACGGC 3 cut(s) 516, 933, 1780
BciT130I CCWGG 4 cut(s) 1084, 1362, 1527, 1559
BclI TGATCA 2 cut(s) 403, 1438
BcoDI GTCTC 5 cut(s) 489, 593, 653, 940, 1071
BfaI CTAG 6 cut(s) 435, 755, 950, 1230, 1310, 1677
BfuAI ACCTGC 1 cut(s) 1502
BlpI GCTNAGC 1 cut(s) 1088
Bme1390I CCNGG 4 cut(s) 1084, 1362, 1527, 1559
Bme18I GGWCC 4 cut(s) 4, 686, 1555, 1663
BmeT110I CYCGRG 2 cut(s) 53, 1204
BmgT120I GGNCC 6 cut(s) 4, 156, 686, 919, 1555, 1663
BmiI GGNNCC 3 cut(s) 1556, 1664, 1926
BmrFI CCNGG 4 cut(s) 1084, 1362, 1527, 1559
BmsI GCATC 5 cut(s) 211, 309, 1021, 1573, 1882
BoxI GACNNNNGTC 1 cut(s) 330
BpiI GAAGAC 2 cut(s) 920, 1639
Bpu1102I GCTNAGC 1 cut(s) 1088
Bpu14I TTCGAA 1 cut(s) 1340
BpuEI CTTGAG 1 cut(s) 677
BsaAI YACGTR 1 cut(s) 1174
BsaBI GATNNNNATC 1 cut(s) 735
BsaI GGTCTC 2 cut(s) 593, 1071
BsaJI CCNNGG 2 cut(s) 1082, 1558
Bsc4I CCNNNNNNNGG 3 cut(s) 538, 1564, 1660
Bse1I ACTGG 5 cut(s) 136, 146, 772, 1587, 1635
Bse21I CCTNAGG 1 cut(s) 567
Bse3DI GCAATG 6 cut(s) 704, 927, 976, 1099, 1803, 2015
Bse8I GATNNNNATC 1 cut(s) 735
BseBI CCWGG 4 cut(s) 1084, 1362, 1527, 1559
BseDI CCNNGG 2 cut(s) 1082, 1558
BseGI GGATG 3 cut(s) 812, 1650, 1968
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 3 cut(s) 538, 1564, 1660
BseMI GCAATG 6 cut(s) 704, 927, 976, 1099, 1803, 2015
BseMII CTCAG 1 cut(s) 558
BseNI ACTGG 5 cut(s) 136, 146, 772, 1587, 1635
BseXI GCAGC 8 cut(s) 92, 453, 604, 814, 871, 1135, 1490, 2014
BseYI CCCAGC 1 cut(s) 1707
Bsh1236I CGCG 3 cut(s) 389, 1306, 1974
Bsh1285I CGRYCG 2 cut(s) 278, 733
BshFI GGCC 6 cut(s) 158, 386, 503, 840, 920, 1971
BshNI GGYRCC 1 cut(s) 1924
BsiEI CGRYCG 2 cut(s) 278, 733
BsiHKAI GWGCWC 1 cut(s) 442
BsiHKCI CYCGRG 2 cut(s) 53, 1204
BslFI GGGAC 1 cut(s) 1568
BslI CCNNNNNNNGG 3 cut(s) 538, 1564, 1660
BsmAI GTCTC 5 cut(s) 489, 593, 653, 940, 1071
BsmFI GGGAC 1 cut(s) 1568
BsmI GAATGC 3 cut(s) 128, 1247, 1934
BsnI GGCC 6 cut(s) 158, 386, 503, 840, 920, 1971
Bso31I GGTCTC 2 cut(s) 593, 1071
BsoBI CYCGRG 2 cut(s) 53, 1204
Bsp119I TTCGAA 1 cut(s) 1340
Bsp1286I GDGCHC 1 cut(s) 442
Bsp1407I TGTACA 1 cut(s) 1821
Bsp143I GATC 7 cut(s) 67, 139, 403, 720, 730, 736, 1438
Bsp1720I GCTNAGC 1 cut(s) 1088
Bsp68I TCGCGA 1 cut(s) 1306
BspACI CCGC 7 cut(s) 159, 387, 1530, 1972, 1988, 1996, 2005
BspANI GGCC 6 cut(s) 158, 386, 503, 840, 920, 1971
BspCNI CTCAG 1 cut(s) 559
BspFNI CGCG 3 cut(s) 389, 1306, 1974
BspHI TCATGA 2 cut(s) 487, 741
BspLI GGNNCC 3 cut(s) 1556, 1664, 1926
BspMI ACCTGC 1 cut(s) 1502
BspPI GGATC 3 cut(s) 75, 147, 744
BspT104I TTCGAA 1 cut(s) 1340
BspT107I GGYRCC 1 cut(s) 1924
BspTNI GGTCTC 2 cut(s) 593, 1071
BsrDI GCAATG 6 cut(s) 704, 927, 976, 1099, 1803, 2015
BsrGI TGTACA 1 cut(s) 1821
BsrI ACTGG 5 cut(s) 136, 146, 772, 1587, 1635
BssECI CCNNGG 2 cut(s) 1082, 1558
BssMI GATC 7 cut(s) 67, 139, 403, 720, 730, 736, 1438
BssNAI GTATAC 2 cut(s) 151, 1686
Bst1107I GTATAC 2 cut(s) 151, 1686
Bst2UI CCWGG 4 cut(s) 1084, 1362, 1527, 1559
Bst4CI ACNGT 8 cut(s) 238, 279, 310, 481, 538, 1352, 1375, 1498
Bst6I CTCTTC 1 cut(s) 1342
BstAPI GCANNNNNTGC 1 cut(s) 86
BstAUI TGTACA 1 cut(s) 1821
BstBAI YACGTR 1 cut(s) 1174
BstBI TTCGAA 1 cut(s) 1340
BstC8I GCNNGC 8 cut(s) 259, 298, 351, 464, 711, 995, 1990, 1994
BstDEI CTNAG 4 cut(s) 567, 866, 1088, 1958
BstF5I GGATG 3 cut(s) 812, 1650, 1968
BstFNI CGCG 3 cut(s) 389, 1306, 1974
BstHHI GCGC 2 cut(s) 263, 522
BstKTI GATC 7 cut(s) 70, 142, 406, 723, 733, 739, 1441
BstMAI GTCTC 5 cut(s) 489, 593, 653, 940, 1071
BstMBI GATC 7 cut(s) 67, 139, 403, 720, 730, 736, 1438
BstMCI CGRYCG 2 cut(s) 278, 733
BstMWI GCNNNNNNNGC 7 cut(s) 80, 86, 132, 590, 710, 1805, 2002
BstNI CCWGG 4 cut(s) 1084, 1362, 1527, 1559
BstNSI RCATGY 5 cut(s) 261, 331, 383, 1831, 1951
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 4 cut(s) 1082, 1360, 1525, 1557
BstUI CGCG 3 cut(s) 389, 1306, 1974
BstV1I GCAGC 8 cut(s) 92, 453, 604, 814, 871, 1135, 1490, 2014
BstV2I GAAGAC 2 cut(s) 920, 1639
BstX2I RGATCY 2 cut(s) 139, 736
BstYI RGATCY 2 cut(s) 139, 736
BstZ17I GTATAC 2 cut(s) 151, 1686
Bsu36I CCTNAGG 1 cut(s) 567
BsuRI GGCC 6 cut(s) 158, 386, 503, 840, 920, 1971
BtgZI GCGATG 1 cut(s) 1989
BtsCI GGATG 3 cut(s) 812, 1650, 1968
BtsI GCAGTG 3 cut(s) 338, 522, 1671
BtsIMutI CAGTG 7 cut(s) 338, 522, 534, 765, 1357, 1371, 1671
BtuMI TCGCGA 1 cut(s) 1306
BveI ACCTGC 1 cut(s) 1502
Cac8I GCNNGC 8 cut(s) 259, 298, 351, 464, 711, 995, 1990, 1994
CaiI CAGNNNCTG 1 cut(s) 566
CciI TCATGA 2 cut(s) 487, 741
CfoI GCGC 2 cut(s) 263, 522
Cfr13I GGNCC 6 cut(s) 4, 156, 686, 919, 1555, 1663
CseI GACGC 1 cut(s) 1235
CsiI ACCWGGT 1 cut(s) 1360
Csp6I GTAC 5 cut(s) 577, 1058, 1697, 1822, 1887
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviQI GTAC 5 cut(s) 577, 1058, 1697, 1822, 1887
DdeI CTNAG 4 cut(s) 567, 866, 1088, 1958
DpnI GATC 7 cut(s) 69, 141, 405, 722, 732, 738, 1440
DpnII GATC 7 cut(s) 67, 139, 403, 720, 730, 736, 1438
EaeI YGGCCR 2 cut(s) 384, 838
Eam1104I CTCTTC 1 cut(s) 1342
EarI CTCTTC 1 cut(s) 1342
Ecl136II GAGCTC 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 442
Eco31I GGTCTC 2 cut(s) 593, 1071
Eco47I GGWCC 4 cut(s) 4, 686, 1555, 1663
Eco53kI GAGCTC 1 cut(s) 440
Eco72I CACGTG 1 cut(s) 1174
Eco81I CCTNAGG 1 cut(s) 567
Eco88I CYCGRG 2 cut(s) 53, 1204
EcoICRI GAGCTC 1 cut(s) 440
EcoO109I RGGNCCY 2 cut(s) 686, 1663
EcoRII CCWGG 4 cut(s) 1082, 1360, 1525, 1557
EcoT22I ATGCAT 1 cut(s) 1745
EcoT38I GRGCYC 1 cut(s) 442
FalI AAGNNNNNCTT 2 cut(s) 1321, 1353
FaqI GGGAC 1 cut(s) 1568
FauI CCCGC 1 cut(s) 1981
FbaI TGATCA 2 cut(s) 403, 1438
FblI GTMKAC 3 cut(s) 150, 1002, 1685
FokI GGATG 3 cut(s) 819, 1637, 1975
FriOI GRGCYC 1 cut(s) 442
FspBI CTAG 6 cut(s) 435, 755, 950, 1230, 1310, 1677
FspI TGCGCA 1 cut(s) 262
GlaI GCGC 2 cut(s) 262, 521
GsaI CCCAGC 1 cut(s) 1711
HaeIII GGCC 6 cut(s) 158, 386, 503, 840, 920, 1971
HgaI GACGC 1 cut(s) 1235
HhaI GCGC 2 cut(s) 263, 522
Hin6I GCGC 2 cut(s) 261, 520
HinP1I GCGC 2 cut(s) 261, 520
HincII GTYRAC 1 cut(s) 1003
HindII GTYRAC 1 cut(s) 1003
HindIII AAGCTT 1 cut(s) 453
HphI GGTGA 6 cut(s) 50, 377, 521, 730, 782, 1019
Hpy166II GTNNAC 4 cut(s) 151, 1003, 1348, 1686
Hpy188I TCNGA 5 cut(s) 25, 286, 649, 1074, 1192
Hpy8I GTNNAC 4 cut(s) 151, 1003, 1348, 1686
HpyAV CCTTC 9 cut(s) 21, 58, 677, 932, 1420, 1444, 1513, 1676, 1961
HpyCH4III ACNGT 8 cut(s) 238, 279, 310, 481, 538, 1352, 1375, 1498
HpyCH4IV ACGT 1 cut(s) 1173
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 86, 132, 590, 710, 1805, 2002
HpyF3I CTNAG 4 cut(s) 567, 866, 1088, 1958
HpySE526I ACGT 1 cut(s) 1173
HspAI GCGC 2 cut(s) 261, 520
Ksp22I TGATCA 2 cut(s) 403, 1438
Kzo9I GATC 7 cut(s) 67, 139, 403, 720, 730, 736, 1438
Lsp1109I GCAGC 8 cut(s) 92, 453, 604, 814, 871, 1135, 1490, 2014
LweI GCATC 5 cut(s) 211, 309, 1021, 1573, 1882
MabI ACCWGGT 1 cut(s) 1360
MaeI CTAG 6 cut(s) 435, 755, 950, 1230, 1310, 1677
MaeII ACGT 1 cut(s) 1173
MaeIII GTNAC 7 cut(s) 189, 310, 896, 1169, 1223, 1498, 1606
MalI GATC 7 cut(s) 69, 141, 405, 722, 732, 738, 1440
MboI GATC 7 cut(s) 67, 139, 403, 720, 730, 736, 1438
MboII GAAGA 7 cut(s) 12, 134, 925, 1016, 1327, 1329, 1639
MfeI CAATTG 1 cut(s) 1035
MflI RGATCY 2 cut(s) 139, 736
MhlI GDGCHC 1 cut(s) 442
MlyI GAGTC 6 cut(s) 214, 805, 887, 1186, 1196, 1326
MmeI TCCRAC 1 cut(s) 248
Mph1103I ATGCAT 1 cut(s) 1745
MseI TTAA 6 cut(s) 128, 848, 1103, 1457, 1616, 1881
MslI CAYNNNNRTG 4 cut(s) 399, 539, 951, 1738
MspA1I CMGCKG 2 cut(s) 466, 2005
MspR9I CCNGG 4 cut(s) 1084, 1362, 1527, 1559
MunI CAATTG 1 cut(s) 1035
Mva1269I GAATGC 3 cut(s) 128, 1247, 1934
MvaI CCWGG 4 cut(s) 1084, 1362, 1527, 1559
MvnI CGCG 3 cut(s) 389, 1306, 1974
MwoI GCNNNNNNNGC 7 cut(s) 80, 86, 132, 590, 710, 1805, 2002
NdeII GATC 7 cut(s) 67, 139, 403, 720, 730, 736, 1438
NlaIV GGNNCC 3 cut(s) 1556, 1664, 1926
NmuCI GTSAC 4 cut(s) 896, 1169, 1223, 1606
NruI TCGCGA 1 cut(s) 1306
NsbI TGCGCA 1 cut(s) 262
NsiI ATGCAT 1 cut(s) 1745
NspI RCATGY 5 cut(s) 261, 331, 383, 1831, 1951
NspV TTCGAA 1 cut(s) 1340
OliI CACNNNNGTG 1 cut(s) 539
PaeI GCATGC 1 cut(s) 261
PaeR7I CTCGAG 2 cut(s) 53, 1204
PagI TCATGA 2 cut(s) 487, 741
PciI ACATGT 2 cut(s) 327, 379
PctI GAATGC 3 cut(s) 128, 1247, 1934
PfeI GAWTC 5 cut(s) 92, 318, 1282, 1702, 1787
PflMI CCANNNNNTGG 1 cut(s) 1564
Ple19I CGATCG 1 cut(s) 733
PleI GAGTC 6 cut(s) 214, 805, 886, 1185, 1196, 1326
PmaCI CACGTG 1 cut(s) 1174
PmlI CACGTG 1 cut(s) 1174
PpsI GAGTC 6 cut(s) 214, 805, 886, 1185, 1196, 1326
Ppu21I YACGTR 1 cut(s) 1174
PpuMI RGGWCCY 2 cut(s) 686, 1663
PscI ACATGT 2 cut(s) 327, 379
PshAI GACNNNNGTC 1 cut(s) 330
PsiI TTATAA 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 442
Psp5II RGGWCCY 2 cut(s) 686, 1663
Psp6I CCWGG 4 cut(s) 1082, 1360, 1525, 1557
PspCI CACGTG 1 cut(s) 1174
PspFI CCCAGC 1 cut(s) 1707
PspGI CCWGG 4 cut(s) 1082, 1360, 1525, 1557
PspN4I GGNNCC 3 cut(s) 1556, 1664, 1926
PspPI GGNCC 6 cut(s) 4, 156, 686, 919, 1555, 1663
PspPPI RGGWCCY 2 cut(s) 686, 1663
PspXI VCTCGAGB 1 cut(s) 1204
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 2 cut(s) 139, 736
PvuI CGATCG 1 cut(s) 733
PvuII CAGCTG 1 cut(s) 466
RruI TCGCGA 1 cut(s) 1306
RsaI GTAC 5 cut(s) 578, 1059, 1698, 1823, 1888
RsaNI GTAC 5 cut(s) 577, 1058, 1697, 1822, 1887
RseI CAYNNNNRTG 4 cut(s) 399, 539, 951, 1738
SacI GAGCTC 1 cut(s) 442
SalI GTCGAC 1 cut(s) 1001
SaqAI TTAA 6 cut(s) 128, 848, 1103, 1457, 1616, 1881
Sau3AI GATC 7 cut(s) 67, 139, 403, 720, 730, 736, 1438
Sau96I GGNCC 6 cut(s) 4, 156, 686, 919, 1555, 1663
SchI GAGTC 6 cut(s) 214, 805, 887, 1186, 1196, 1326
ScrFI CCNGG 4 cut(s) 1084, 1362, 1527, 1559
SduI GDGCHC 1 cut(s) 442
SexAI ACCWGGT 1 cut(s) 1360
SfaNI GCATC 5 cut(s) 211, 309, 1021, 1573, 1882
Sfr274I CTCGAG 2 cut(s) 53, 1204
SfuI TTCGAA 1 cut(s) 1340
SinI GGWCC 4 cut(s) 4, 686, 1555, 1663
SlaI CTCGAG 2 cut(s) 53, 1204
SmiMI CAYNNNNRTG 4 cut(s) 399, 539, 951, 1738
SmlI CTYRAG 3 cut(s) 53, 656, 1204
SmoI CTYRAG 3 cut(s) 53, 656, 1204
SphI GCATGC 1 cut(s) 261
SsiI CCGC 7 cut(s) 159, 387, 1530, 1972, 1988, 1996, 2005
SspI AATATT 4 cut(s) 175, 216, 1021, 1870
SspMI CTAG 6 cut(s) 435, 755, 950, 1230, 1310, 1677
SstI GAGCTC 1 cut(s) 442
StyD4I CCNGG 4 cut(s) 1082, 1360, 1525, 1557
TaaI ACNGT 8 cut(s) 238, 279, 310, 481, 538, 1352, 1375, 1498
TaiI ACGT 1 cut(s) 1176
TaqI TCGA 5 cut(s) 54, 324, 1002, 1205, 1340
TatI WGTACW 3 cut(s) 576, 1057, 1821
TauI GCSGC 4 cut(s) 161, 389, 1974, 2008
TfiI GAWTC 5 cut(s) 92, 318, 1282, 1702, 1787
Tru1I TTAA 6 cut(s) 128, 848, 1103, 1457, 1616, 1881
Tru9I TTAA 6 cut(s) 128, 848, 1103, 1457, 1616, 1881
TscAI CASTG 7 cut(s) 345, 522, 541, 772, 1357, 1378, 1678
TseFI GTSAC 4 cut(s) 896, 1169, 1223, 1606
TseI GCWGC 8 cut(s) 80, 466, 617, 827, 884, 1148, 1478, 2002
Tsp45I GTSAC 4 cut(s) 896, 1169, 1223, 1606
TspDTI ATGAA 9 cut(s) 135, 603, 696, 974, 1127, 1207, 1591, 1639, 1943
TspGWI ACGGA 4 cut(s) 210, 507, 1157, 1928
TspRI CASTG 7 cut(s) 345, 522, 541, 772, 1357, 1378, 1678
Van91I CCANNNNNTGG 1 cut(s) 1564
VpaK11BI GGWCC 4 cut(s) 4, 686, 1555, 1663
XapI RAATTY 2 cut(s) 1163, 1612
XbaI TCTAGA 1 cut(s) 949
XceI RCATGY 5 cut(s) 261, 331, 383, 1831, 1951
XhoI CTCGAG 2 cut(s) 53, 1204
XmiI GTMKAC 3 cut(s) 150, 1002, 1685
XspI CTAG 6 cut(s) 435, 755, 950, 1230, 1310, 1677
Zsp2I ATGCAT 1 cut(s) 1745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.