pycom08g18270

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000489
Physical Location & Seq
Forward (+)
18531113 .. 18531829
717 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g18270.1

Sequence Viewer

Length: 717 bp
ATGGATACAGAGATCAAATCAGGTACCAGAACATTGACGAAGTACCCATACATGGATCCAAATACCTACAAGGCAGCAGCAAAAGGCAAAATTGAGATTTTTAAGGAAATCAGCCAGCAGCAGCATCTCGGCCGCCTATTAACCCCAAACAAGAACACAGTCCTTCATATCAATATCGCGGCCCAAAAAGGAGGTGGAACTGACTACTCAACCGACTTTGTGAAGCAGGTGCTTCATATATATCCATCACTGTTACTCCAAGTTAATATCAAAGGTGAGAGTGCACTGCACATTGCGGCAAGGCACGGCCATGTTGCTGCAGTCAAAGTCCTTCTTGAGTTCGCAAAAGTTGCTCAATCTCACGGTGATCACACTGATGAGCTTGAGAGTGGCATGGTCCGAGGAGATGATCATGAACAAATATCGGTATTGTTGCGCATGAAGAGTAACAAGAATGATACAGCCTTGCATGAGGCAGCACGCTACAATCGACTTGGTGTTGTGCAAGTACTGACCCAAGAAGACCCTGATTTTCCATACTCTCCTAATGAAGCTGGCGAAACTCCACTTTACCTCGCTGCTGAGAGAGGGTACCGTGAAGTAGTGTTTGAAATGTTACAAACTTGTACTTCACCAGATCATAGAGGACCCAATGGTAGAACGACTCTGCATGCTGCAATAATTTTCAAAGATGGAGGTACCATACAATACGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

239

Amino Acids

26.43

Weight (kDa)

6.63

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 24 - 113 4.8e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 217
Acc16I TGCGCA 1 cut(s) 437
Acc36I ACCTGC 1 cut(s) 217
Acc65I GGTACC 3 cut(s) 23, 591, 698
AccB1I GGYRCC 3 cut(s) 23, 591, 698
AccII CGCG 1 cut(s) 179
AciI CCGC 3 cut(s) 133, 179, 296
AclWI GGATC 2 cut(s) 50, 63
AcoI YGGCCR 2 cut(s) 130, 307
AfaI GTAC 6 cut(s) 25, 44, 510, 593, 628, 700
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 2 cut(s) 611, 688
AluBI AGCT 2 cut(s) 382, 554
AluI AGCT 2 cut(s) 382, 554
Alw21I GWGCWC 1 cut(s) 286
Alw44I GTGCAC 1 cut(s) 282
AlwI GGATC 2 cut(s) 50, 63
AoxI GGCC 3 cut(s) 130, 180, 307
ApaLI GTGCAC 1 cut(s) 282
ApeKI GCWGC 8 cut(s) 74, 77, 118, 121, 317, 476, 578, 674
Asp718I GGTACC 3 cut(s) 23, 591, 698
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 3 cut(s) 181, 397, 647
AsuHPI GGTGA 3 cut(s) 287, 377, 624
AvaII GGWCC 2 cut(s) 397, 647
BaeGI GKGCMC 1 cut(s) 286
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 3 cut(s) 23, 591, 698
BarI GAAGNNNNNNTAC 2 cut(s) 32, 64
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 286
BbvI GCAGC 8 cut(s) 86, 89, 130, 133, 304, 488, 565, 661
BccI CCATC 2 cut(s) 253, 686
BceAI ACGGC 1 cut(s) 322
BclI TGATCA 2 cut(s) 367, 409
BfmI CTRYAG 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 217
BmcAI AGTACT 1 cut(s) 510
Bme18I GGWCC 2 cut(s) 397, 647
BmgT120I GGNCC 3 cut(s) 181, 397, 647
BmiI GGNNCC 5 cut(s) 25, 57, 593, 649, 700
BmsI GCATC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 528
BpuEI CTTGAG 2 cut(s) 356, 404
BsaJI CCNNGG 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 400
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 573
BseRI GAGGAG 1 cut(s) 417
BseSI GKGCMC 1 cut(s) 286
BseX3I CGGCCG 1 cut(s) 130
BseXI GCAGC 8 cut(s) 86, 89, 130, 133, 304, 488, 565, 661
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 1 cut(s) 179
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 3 cut(s) 132, 182, 309
BshNI GGYRCC 3 cut(s) 23, 591, 698
BsiEI CGRYCG 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 286
BslI CCNNNNNNNGG 1 cut(s) 52
BsnI GGCC 3 cut(s) 132, 182, 309
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 5 cut(s) 12, 55, 367, 409, 637
BspACI CCGC 3 cut(s) 133, 179, 296
BspANI GGCC 3 cut(s) 132, 182, 309
BspCNI CTCAG 1 cut(s) 574
BspFNI CGCG 1 cut(s) 179
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 5 cut(s) 25, 57, 593, 649, 700
BspMAI CTGCAG 1 cut(s) 322
BspMI ACCTGC 1 cut(s) 217
BspPI GGATC 2 cut(s) 50, 63
BspT107I GGYRCC 3 cut(s) 23, 591, 698
BsrDI GCAATG 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 5 cut(s) 12, 55, 367, 409, 637
Bst4CI ACNGT 4 cut(s) 160, 252, 365, 596
Bst6I CTCTTC 1 cut(s) 437
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 4 cut(s) 116, 481, 556, 672
BstDEI CTNAG 1 cut(s) 582
BstFNI CGCG 1 cut(s) 179
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 5 cut(s) 15, 58, 370, 412, 640
BstMBI GATC 5 cut(s) 12, 55, 367, 409, 637
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 1 cut(s) 350
BstNSI RCATGY 1 cut(s) 674
BstSFI CTRYAG 1 cut(s) 318
BstSLI GKGCMC 1 cut(s) 286
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 8 cut(s) 86, 89, 130, 133, 304, 488, 565, 661
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BstZI CGGCCG 1 cut(s) 130
BsuRI GGCC 3 cut(s) 132, 182, 309
BtsI GCAGTG 1 cut(s) 284
BtsIMutI CAGTG 3 cut(s) 248, 284, 372
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 4 cut(s) 116, 481, 556, 672
CciI TCATGA 1 cut(s) 412
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 3 cut(s) 181, 397, 647
Csp6I GTAC 6 cut(s) 24, 43, 509, 592, 627, 699
CviAII CATG 7 cut(s) 52, 311, 394, 413, 439, 470, 671
CviJI RGCY 7 cut(s) 114, 132, 182, 309, 382, 464, 554
CviKI_1 RGCY 7 cut(s) 114, 132, 182, 309, 382, 464, 554
CviQI GTAC 6 cut(s) 24, 43, 509, 592, 627, 699
DdeI CTNAG 1 cut(s) 582
DpnI GATC 5 cut(s) 14, 57, 369, 411, 639
DpnII GATC 5 cut(s) 12, 55, 367, 409, 637
EaeI YGGCCR 2 cut(s) 130, 307
EagI CGGCCG 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
EclXI CGGCCG 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 397, 647
Eco52I CGGCCG 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 647
FaeI CATG 7 cut(s) 55, 314, 397, 416, 442, 473, 674
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FatI CATG 7 cut(s) 51, 310, 393, 412, 438, 469, 670
FbaI TGATCA 2 cut(s) 367, 409
FspI TGCGCA 1 cut(s) 437
GlaI GCGC 1 cut(s) 437
HaeIII GGCC 3 cut(s) 132, 182, 309
HhaI GCGC 1 cut(s) 438
Hin1II CATG 7 cut(s) 55, 314, 397, 416, 442, 473, 674
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HinfI GANTC 1 cut(s) 664
HphI GGTGA 3 cut(s) 287, 377, 624
Hpy166II GTNNAC 1 cut(s) 284
Hpy188I TCNGA 1 cut(s) 401
Hpy188III TCNNGA 2 cut(s) 335, 413
Hpy8I GTNNAC 1 cut(s) 284
HpyAV CCTTC 2 cut(s) 173, 341
HpyCH4III ACNGT 4 cut(s) 160, 252, 365, 596
HpyCH4IV ACGT 1 cut(s) 711
HpyCH4V TGCA 7 cut(s) 284, 289, 320, 469, 505, 670, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 350
HpyF3I CTNAG 1 cut(s) 582
HpySE526I ACGT 1 cut(s) 711
Hsp92II CATG 7 cut(s) 55, 314, 397, 416, 442, 473, 674
HspAI GCGC 1 cut(s) 436
KpnI GGTACC 3 cut(s) 27, 595, 702
Ksp22I TGATCA 2 cut(s) 367, 409
Kzo9I GATC 5 cut(s) 12, 55, 367, 409, 637
LpnPI CCDG 7 cut(s) 6, 40, 128, 212, 540, 540, 648
Lsp1109I GCAGC 8 cut(s) 86, 89, 130, 133, 304, 488, 565, 661
LweI GCATC 1 cut(s) 133
MaeII ACGT 1 cut(s) 711
MaeIII GTNAC 3 cut(s) 252, 446, 615
MalI GATC 5 cut(s) 14, 57, 369, 411, 639
MboI GATC 5 cut(s) 12, 55, 367, 409, 637
MboII GAAGA 2 cut(s) 454, 533
MflI RGATCY 1 cut(s) 55
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 2 cut(s) 90, 681
MlyI GAGTC 1 cut(s) 658
MnlI CCTC 7 cut(s) 185, 395, 466, 581, 584, 638, 689
MseI TTAA 3 cut(s) 102, 140, 264
MslI CAYNNNNRTG 2 cut(s) 309, 375
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 1 cut(s) 350
NdeII GATC 5 cut(s) 12, 55, 367, 409, 637
NlaIII CATG 7 cut(s) 55, 314, 397, 416, 442, 473, 674
NlaIV GGNNCC 5 cut(s) 25, 57, 593, 649, 700
NmeAIII GCCGAG 1 cut(s) 108
NsbI TGCGCA 1 cut(s) 437
NspI RCATGY 1 cut(s) 674
PaeI GCATGC 1 cut(s) 674
PagI TCATGA 1 cut(s) 412
PaqCI CACCTGC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 487
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
PpuMI RGGWCCY 1 cut(s) 647
Psp5II RGGWCCY 1 cut(s) 647
PspN4I GGNNCC 5 cut(s) 25, 57, 593, 649, 700
PspPI GGNCC 3 cut(s) 181, 397, 647
PspPPI RGGWCCY 1 cut(s) 647
PstI CTGCAG 1 cut(s) 322
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 6 cut(s) 25, 44, 510, 593, 628, 700
RsaNI GTAC 6 cut(s) 24, 43, 509, 592, 627, 699
RseI CAYNNNNRTG 2 cut(s) 309, 375
SaqAI TTAA 3 cut(s) 102, 140, 264
Sau3AI GATC 5 cut(s) 12, 55, 367, 409, 637
Sau96I GGNCC 3 cut(s) 181, 397, 647
ScaI AGTACT 1 cut(s) 510
SchI GAGTC 1 cut(s) 658
SduI GDGCHC 1 cut(s) 286
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 1 cut(s) 318
SinI GGWCC 2 cut(s) 397, 647
SmiMI CAYNNNNRTG 2 cut(s) 309, 375
SmlI CTYRAG 2 cut(s) 335, 383
SmoI CTYRAG 2 cut(s) 335, 383
SphI GCATGC 1 cut(s) 674
Sse9I AATT 2 cut(s) 90, 681
SsiI CCGC 3 cut(s) 133, 179, 296
TaaI ACNGT 4 cut(s) 160, 252, 365, 596
TaiI ACGT 1 cut(s) 714
TaqI TCGA 1 cut(s) 490
TasI AATT 2 cut(s) 90, 681
TatI WGTACW 2 cut(s) 508, 626
TauI GCSGC 3 cut(s) 135, 182, 299
Tru1I TTAA 3 cut(s) 102, 140, 264
Tru9I TTAA 3 cut(s) 102, 140, 264
TscAI CASTG 3 cut(s) 255, 291, 379
TseI GCWGC 8 cut(s) 74, 77, 118, 121, 317, 476, 578, 674
TspDTI ATGAA 5 cut(s) 155, 224, 429, 455, 564
TspRI CASTG 3 cut(s) 255, 291, 379
VneI GTGCAC 1 cut(s) 282
VpaK11BI GGWCC 2 cut(s) 397, 647
XceI RCATGY 1 cut(s) 674
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
ZrmI AGTACT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.