Rw1G008050

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000489
Physical Location & Seq
Forward (+)
17276435 .. 17281356
4922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G008050.1

Sequence Viewer

Length: 1914 bp
ATGGATAATGATACATACCAGGCAGCAAAAGAAGGCAACATCGATGGTCTTCAGAGAATAGAGGATCTTTGCCTATCGAGTCCCATGGGTAACACGTTCCTCCATATTTATGTAGCCTGCCAAACATCAACTGATCCTGTAGAAGTACTGAAGTCAACCGACACGGTCAAGAGGATTCTTCAAGAATGTCCTGCGCTGCTAATGCAGCAGAACAAGAGTGGTGAGACCGCATTACACATTGCAGCGAAACACGGACGTGCTGCCATTGCTAAAGTTTTAATTGATCAGGTTGCGAAAGCCAAAAATGGTAATGACCTTGAGGAAGGGGTGTCAGCGACAGAAGCCTTCTTGTCTGCGCAGAAGTTCATCAGGAAAACTAACAAGGAGAAGGACACCGCCTTACACGAGGCAGCACGCTTTAATCACCTCAATGTCGTGGAGCTGTTGATTGGTGGCGATCGTGAATTTTCCTGCTCTCATAACAATGCTGGGAAGAATCCACCGTCCTCGGTTGTCAACAAAGTATTTGATGAATTATTTTCTCGAATATCTCTCATTCTCGGAAATCCAACTTACCAAGGCCCCGATCGTCGATTTTCCTACTCTCCTAACAATGCTGGAGAGACTCCATTATACTTGGCTGCCGAAAGAGGACATCATGAATTAGTTTCTAAAATATTGCACTTTTGCCAAAATCCAACTTACCAAGGCCCCGATGGTAGAACAGCTCTACATGCTGCAGTGATTTGCAATCATGAAGAAATAACGATAAAATTACTAGAGCACGAAGGTTGGACTGCACCAAGGTCGGAACGTTGGAATTATAACCTATCATCTGAGTTGACGGATGTGGTAGATACACGAGGATGGACTCCACTTCACTGGGCTGTATTTTTGGGCCACACTTCTATTGTAAAACGACTACTACTATCAAATAAACGTGCTGCTTATATTGGTGACAAGGAAGACAAGAAGACGGCTCTTCACATTGCAGCCTCCAAAGGCCATGTAGATATAATGAAAGAGCTCCTTTATCATTGTCCTGGTTGTTGTGAACTGGTTGACAAGAGACGACGAAATATTCTTCACTATGCGATTGAGAACCATAAATTTGAAATAGAGCGTTTGGTTCGAGGAGATCCATGGCTTAGTAACGTCCTCTTAAATGGCAAGGATGTTGATGGAAACACACCTCTCCATCAACTTACTGCTTCTCTGTATGGAGGCACTGAATTCATAAGTGATCCTCGGGTTGATAAGATGGCATTCAACAAAAATAACCAGACCGCTTTTGACAACATTCCCACGGATAAGACTTTCCCAGTACAGCAGGAAGATCCTTTACACGTTTTGAAGAATAATGGGGGCAGACCAGGTCATCGAATTGTAAGGAATGTGGAAAACAAAGATGATGAAAGCATCAATGAGTCCAAAGAAGCAAAAAATGCATATCTCATAGTGGCTACACTTATAGCTACGGTAACCTTCGCAGCAGGATTCACCATGCCTGGTGGTTACCAAAATGACAAAGGACCGGACCAAGGTTTTGCAGTTCTAACTAGAAACTTCGCTTTCAAAGTATTTATCATTGCAAATTCAATAGCCATGTCCGCGTCCACTGCCTCCGTCTTTATGCATCTTTTTTTATCGATTCAAACAAAGGCTAAATCGATTGAAACAAAGACAAGAAGGATTAATAAAGGCAGGTTGCAGAATGCTGTACTGTGCACGCTGGTGGCTTTAGTTACAATGGTGGTTGCATTCGCTGCGGGCACCTATGCAGTATTAGGTTATTCATCAGAACTTGCCATTACGTCTTGTATGCTTAGCTGCTCTTATTTCTTCTGTCTCTACTTCTCCGAGATACTACGAAAGCTCCTAGTTACGTGCCAAAAATATTCTATATTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

637

Amino Acids

70.85

Weight (kDa)

8.09

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 7 - 96 1.3e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 52 - 150 8.6e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 60 - 102 7.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 204 - 262 1.1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 283 - 346 1.2e-12 Ankyrin repeats (3 copies)
Ank PF00023 289 - 312 4.8e-06 Ankyrin repeat
Ank_2 PF12796 315 - 373 3.1e-09 Ankyrin repeats (3 copies)
PGG PF13962 477 - 595 1.3e-30 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 825
Acc16I TGCGCA 1 cut(s) 357
Acc36I ACCTGC 1 cut(s) 1695
AccB1I GGYRCC 1 cut(s) 1772
AccII CGCG 1 cut(s) 1613
AciI CCGC 5 cut(s) 228, 396, 1287, 1611, 1769
AclI AACGTT 1 cut(s) 814
AclWI GGATC 5 cut(s) 72, 128, 1133, 1238, 1331
AcsI RAATTY 4 cut(s) 464, 1109, 1232, 1594
AcuI CTGAAG 2 cut(s) 35, 170
AfaI GTAC 3 cut(s) 147, 1326, 1722
AfiI CCNNNNNNNGG 1 cut(s) 1541
AflIII ACRYGT 2 cut(s) 93, 1345
AgsI TTSAA 8 cut(s) 182, 1115, 1270, 1354, 1576, 1599, 1655, 1676
AjiI CACGTC 1 cut(s) 257
AjnI CCWGG 4 cut(s) 18, 1042, 1372, 1507
AleI CACNNNNGTG 2 cut(s) 255, 1733
AluBI AGCT 6 cut(s) 442, 728, 1027, 1475, 1830, 1876
AluI AGCT 6 cut(s) 442, 728, 1027, 1475, 1830, 1876
Alw21I GWGCWC 3 cut(s) 786, 1029, 1730
Alw26I GTCTC 4 cut(s) 218, 617, 1063, 1853
Alw44I GTGCAC 1 cut(s) 1726
AlwI GGATC 5 cut(s) 72, 128, 1133, 1238, 1331
Ama87I CYCGRG 1 cut(s) 1248
AoxI GGCC 4 cut(s) 580, 709, 898, 1003
ApaLI GTGCAC 1 cut(s) 1726
ApoI RAATTY 4 cut(s) 464, 1109, 1232, 1594
ArsI GACNNNNNNTTYG 2 cut(s) 1529, 1561
AseI ATTAAT 1 cut(s) 1695
AspLEI GCGC 2 cut(s) 196, 358
AspS9I GGNCC 5 cut(s) 581, 710, 898, 1532, 1537
AsuHPI GGTGA 4 cut(s) 233, 416, 968, 1492
AvaI CYCGRG 1 cut(s) 1248
AvaII GGWCC 2 cut(s) 1532, 1537
BaeGI GKGCMC 2 cut(s) 1730, 1775
BanI GGYRCC 1 cut(s) 1772
BanII GRGCYC 1 cut(s) 1029
BarI GAAGNNNNNNTAC 2 cut(s) 1326, 1358
BauI CACGAG 2 cut(s) 404, 861
BbsI GAAGAC 3 cut(s) 41, 972, 980
Bbv12I GWGCWC 3 cut(s) 786, 1029, 1730
BccI CCATC 6 cut(s) 38, 710, 861, 1175, 1206, 1255
BceAI ACGGC 1 cut(s) 993
BcgI CGANNNNNNTGC 2 cut(s) 789, 823
BciT130I CCWGG 4 cut(s) 20, 1044, 1374, 1509
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 4 cut(s) 218, 617, 1063, 1853
BfaI CTAG 3 cut(s) 779, 1560, 1880
BfmI CTRYAG 2 cut(s) 138, 738
BfuAI ACCTGC 1 cut(s) 1695
BlpI GCTNAGC 1 cut(s) 1826
BmcAI AGTACT 1 cut(s) 147
Bme1390I CCNGG 4 cut(s) 20, 1044, 1374, 1509
Bme18I GGWCC 2 cut(s) 1532, 1537
BmeT110I CYCGRG 1 cut(s) 1248
BmgBI CACGTC 1 cut(s) 257
BmgT120I GGNCC 5 cut(s) 581, 710, 898, 1532, 1537
BmiI GGNNCC 3 cut(s) 583, 712, 1774
BmrFI CCNGG 4 cut(s) 20, 1044, 1374, 1509
BmrI ACTGGG 2 cut(s) 892, 1316
BmsI GCATC 2 cut(s) 1428, 1645
BmuI ACTGGG 2 cut(s) 892, 1316
BpiI GAAGAC 3 cut(s) 41, 972, 980
BpmI CTGGAG 1 cut(s) 639
Bpu1102I GCTNAGC 1 cut(s) 1826
BpuEI CTTGAG 1 cut(s) 338
Bsa29I ATCGAT 3 cut(s) 42, 1649, 1670
BsaAI YACGTR 1 cut(s) 1887
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 9 cut(s) 84, 507, 577, 706, 803, 1142, 1247, 1305, 1540
BsaWI WCCGGW 1 cut(s) 1534
BsaXI ACNNNNNCTCC 2 cut(s) 1860, 1890
Bsc4I CCNNNNNNNGG 1 cut(s) 1541
Bse1I ACTGG 3 cut(s) 887, 1062, 1322
Bse3DI GCAATG 4 cut(s) 237, 264, 987, 1587
BseBI CCWGG 4 cut(s) 20, 1044, 1374, 1509
BseCI ATCGAT 3 cut(s) 42, 1649, 1670
BseDI CCNNGG 9 cut(s) 84, 507, 577, 706, 803, 1142, 1247, 1305, 1540
BseGI GGATG 3 cut(s) 853, 872, 1180
BseLI CCNNNNNNNGG 1 cut(s) 1541
BseMI GCAATG 4 cut(s) 237, 264, 987, 1587
BseMII CTCAG 1 cut(s) 828
BseNI ACTGG 3 cut(s) 887, 1062, 1322
BseRI GAGGAG 1 cut(s) 1149
BseSI GKGCMC 2 cut(s) 1730, 1775
BseYI CCCAGC 1 cut(s) 488
BsgI GTGCAG 1 cut(s) 783
Bsh1236I CGCG 1 cut(s) 1613
Bsh1285I CGRYCG 2 cut(s) 460, 589
BshFI GGCC 4 cut(s) 582, 711, 900, 1005
BshNI GGYRCC 1 cut(s) 1772
BshVI ATCGAT 3 cut(s) 42, 1649, 1670
BsiEI CGRYCG 2 cut(s) 460, 589
BsiHKAI GWGCWC 3 cut(s) 786, 1029, 1730
BsiHKCI CYCGRG 1 cut(s) 1248
BsiSI CCGG 1 cut(s) 1535
BslFI GGGAC 1 cut(s) 66
BslI CCNNNNNNNGG 1 cut(s) 1541
BsmAI GTCTC 4 cut(s) 218, 617, 1063, 1853
BsmBI CGTCTC 1 cut(s) 1063
BsmFI GGGAC 1 cut(s) 66
BsmI GAATGC 3 cut(s) 1265, 1720, 1760
BsnI GGCC 4 cut(s) 582, 711, 900, 1005
Bso31I GGTCTC 1 cut(s) 218
BsoBI CYCGRG 1 cut(s) 1248
Bsp1286I GDGCHC 4 cut(s) 786, 1029, 1730, 1775
Bsp143I GATC 8 cut(s) 64, 133, 283, 457, 586, 1138, 1243, 1336
Bsp1720I GCTNAGC 1 cut(s) 1826
Bsp19I CCATGG 2 cut(s) 84, 1142
BspACI CCGC 5 cut(s) 228, 396, 1287, 1611, 1769
BspANI GGCC 4 cut(s) 582, 711, 900, 1005
BspCNI CTCAG 1 cut(s) 829
BspDI ATCGAT 3 cut(s) 42, 1649, 1670
BspFNI CGCG 1 cut(s) 1613
BspHI TCATGA 2 cut(s) 658, 754
BspLI GGNNCC 3 cut(s) 583, 712, 1774
BspMAI CTGCAG 1 cut(s) 742
BspMI ACCTGC 1 cut(s) 1695
BspPI GGATC 5 cut(s) 72, 128, 1133, 1238, 1331
BspQI GCTCTTC 1 cut(s) 987
BspT107I GGYRCC 1 cut(s) 1772
BspTNI GGTCTC 1 cut(s) 218
BsrDI GCAATG 4 cut(s) 237, 264, 987, 1587
BsrI ACTGG 3 cut(s) 887, 1062, 1322
BssECI CCNNGG 9 cut(s) 84, 507, 577, 706, 803, 1142, 1247, 1305, 1540
BssMI GATC 8 cut(s) 64, 133, 283, 457, 586, 1138, 1243, 1336
BssSI CACGAG 2 cut(s) 404, 861
BssT1I CCWWGG 6 cut(s) 84, 577, 706, 803, 1142, 1540
Bst2BI CACGAG 2 cut(s) 404, 861
Bst2UI CCWGG 4 cut(s) 20, 1044, 1374, 1509
Bst4CI ACNGT 4 cut(s) 166, 504, 1480, 1725
Bst6I CTCTTC 1 cut(s) 987
BstAPI GCANNNNNTGC 2 cut(s) 1445, 1766
BstBAI YACGTR 1 cut(s) 1887
BstC8I GCNNGC 4 cut(s) 118, 415, 1730, 1771
BstDEI CTNAG 3 cut(s) 837, 1148, 1826
BstDSI CCRYGG 3 cut(s) 84, 1142, 1305
BstEII GGTNACC 2 cut(s) 1480, 1514
BstF5I GGATG 3 cut(s) 853, 872, 1180
BstFNI CGCG 1 cut(s) 1613
BstHHI GCGC 2 cut(s) 196, 358
BstKTI GATC 8 cut(s) 67, 136, 286, 460, 589, 1141, 1246, 1339
BstMAI GTCTC 4 cut(s) 218, 617, 1063, 1853
BstMBI GATC 8 cut(s) 64, 133, 283, 457, 586, 1138, 1243, 1336
BstMCI CGRYCG 2 cut(s) 460, 589
BstMWI GCNNNNNNNGC 9 cut(s) 202, 205, 266, 341, 734, 1445, 1610, 1619, 1766
BstNI CCWGG 4 cut(s) 20, 1044, 1374, 1509
BstNSI RCATGY 1 cut(s) 737
BstPI GGTNACC 2 cut(s) 1480, 1514
BstSCI CCNGG 4 cut(s) 18, 1042, 1372, 1507
BstSFI CTRYAG 2 cut(s) 138, 738
BstSLI GKGCMC 2 cut(s) 1730, 1775
BstUI CGCG 1 cut(s) 1613
BstV2I GAAGAC 3 cut(s) 41, 972, 980
BstX2I RGATCY 3 cut(s) 64, 1138, 1336
BstXI CCANNNNNNTGG 1 cut(s) 882
BstYI RGATCY 3 cut(s) 64, 1138, 1336
Bsu15I ATCGAT 3 cut(s) 42, 1649, 1670
BsuRI GGCC 4 cut(s) 582, 711, 900, 1005
BsuTUI ATCGAT 3 cut(s) 42, 1649, 1670
BtgI CCRYGG 3 cut(s) 84, 1142, 1305
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 3 cut(s) 853, 872, 1180
BtsI GCAGTG 2 cut(s) 747, 1617
BtsIMutI CAGTG 4 cut(s) 747, 880, 1227, 1617
BveI ACCTGC 1 cut(s) 1695
Cac8I GCNNGC 4 cut(s) 118, 415, 1730, 1771
CciI TCATGA 2 cut(s) 658, 754
CfoI GCGC 2 cut(s) 196, 358
Cfr13I GGNCC 5 cut(s) 581, 710, 898, 1532, 1537
ClaI ATCGAT 3 cut(s) 42, 1649, 1670
CseI GACGC 1 cut(s) 1602
CsiI ACCWGGT 1 cut(s) 1372
Csp6I GTAC 3 cut(s) 146, 1325, 1721
CviAII CATG 8 cut(s) 85, 659, 734, 755, 1007, 1143, 1504, 1606
CviQI GTAC 3 cut(s) 146, 1325, 1721
DdeI CTNAG 3 cut(s) 837, 1148, 1826
DpnI GATC 8 cut(s) 66, 135, 285, 459, 588, 1140, 1245, 1338
DpnII GATC 8 cut(s) 64, 133, 283, 457, 586, 1138, 1243, 1336
Eam1104I CTCTTC 1 cut(s) 987
EarI CTCTTC 1 cut(s) 987
Ecl136II GAGCTC 1 cut(s) 1027
Eco130I CCWWGG 6 cut(s) 84, 577, 706, 803, 1142, 1540
Eco24I GRGCYC 1 cut(s) 1029
Eco31I GGTCTC 1 cut(s) 218
Eco47I GGWCC 2 cut(s) 1532, 1537
Eco53kI GAGCTC 1 cut(s) 1027
Eco57I CTGAAG 2 cut(s) 35, 170
Eco88I CYCGRG 1 cut(s) 1248
Eco91I GGTNACC 2 cut(s) 1480, 1514
EcoICRI GAGCTC 1 cut(s) 1027
EcoO109I RGGNCCY 2 cut(s) 581, 710
EcoO65I GGTNACC 2 cut(s) 1480, 1514
EcoRI GAATTC 1 cut(s) 1232
EcoRII CCWGG 4 cut(s) 18, 1042, 1372, 1507
EcoT14I CCWWGG 6 cut(s) 84, 577, 706, 803, 1142, 1540
EcoT22I ATGCAT 2 cut(s) 1450, 1638
EcoT38I GRGCYC 1 cut(s) 1029
ErhI CCWWGG 6 cut(s) 84, 577, 706, 803, 1142, 1540
Esp3I CGTCTC 1 cut(s) 1063
FaeI CATG 8 cut(s) 88, 662, 737, 758, 1010, 1146, 1507, 1609
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FaqI GGGAC 1 cut(s) 66
FatI CATG 8 cut(s) 84, 658, 733, 754, 1006, 1142, 1503, 1605
FauI CCCGC 1 cut(s) 1762
FbaI TGATCA 1 cut(s) 283
FokI GGATG 3 cut(s) 860, 879, 1187
FriOI GRGCYC 1 cut(s) 1029
FspBI CTAG 3 cut(s) 779, 1560, 1880
FspI TGCGCA 1 cut(s) 357
GlaI GCGC 2 cut(s) 195, 357
GsaI CCCAGC 1 cut(s) 492
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 4 cut(s) 582, 711, 900, 1005
HapII CCGG 1 cut(s) 1535
HgaI GACGC 1 cut(s) 1602
HhaI GCGC 2 cut(s) 196, 358
Hin1II CATG 8 cut(s) 88, 662, 737, 758, 1010, 1146, 1507, 1609
Hin6I GCGC 2 cut(s) 194, 356
HinP1I GCGC 2 cut(s) 194, 356
HincII GTYRAC 4 cut(s) 156, 517, 843, 1063
HindII GTYRAC 4 cut(s) 156, 517, 843, 1063
HinfI GANTC 8 cut(s) 79, 175, 496, 625, 871, 1427, 1497, 1651
HpaII CCGG 1 cut(s) 1535
HphI GGTGA 4 cut(s) 233, 416, 968, 1492
Hpy166II GTNNAC 7 cut(s) 156, 517, 843, 1055, 1063, 1617, 1728
Hpy188I TCNGA 6 cut(s) 54, 563, 811, 838, 1801, 1861
Hpy188III TCNNGA 7 cut(s) 169, 182, 370, 461, 543, 659, 755
Hpy8I GTNNAC 7 cut(s) 156, 517, 843, 1055, 1063, 1617, 1728
Hpy99I CGWCG 2 cut(s) 594, 1077
HpyAV CCTTC 7 cut(s) 26, 317, 355, 382, 782, 1495, 1683
HpyCH4III ACNGT 4 cut(s) 166, 504, 1480, 1725
HpyCH4IV ACGT 8 cut(s) 95, 256, 814, 940, 1155, 1347, 1814, 1886
HpyF10VI GCNNNNNNNGC 9 cut(s) 202, 205, 266, 341, 734, 1445, 1610, 1619, 1766
HpyF3I CTNAG 3 cut(s) 837, 1148, 1826
HpySE526I ACGT 8 cut(s) 95, 256, 814, 940, 1155, 1347, 1814, 1886
Hsp92II CATG 8 cut(s) 88, 662, 737, 758, 1010, 1146, 1507, 1609
HspAI GCGC 2 cut(s) 194, 356
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 8 cut(s) 64, 133, 283, 457, 586, 1138, 1243, 1336
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 3 cut(s) 439, 1032, 1881
LweI GCATC 2 cut(s) 1428, 1645
MabI ACCWGGT 1 cut(s) 1372
MaeI CTAG 3 cut(s) 779, 1560, 1880
MaeII ACGT 8 cut(s) 95, 256, 814, 940, 1155, 1347, 1814, 1886
MaeIII GTNAC 7 cut(s) 89, 956, 1151, 1480, 1514, 1744, 1882
MalI GATC 8 cut(s) 66, 135, 285, 459, 588, 1140, 1245, 1338
MboI GATC 8 cut(s) 64, 133, 283, 457, 586, 1138, 1243, 1336
MflI RGATCY 3 cut(s) 64, 1138, 1336
MhlI GDGCHC 4 cut(s) 786, 1029, 1730, 1775
MlyI GAGTC 4 cut(s) 88, 619, 865, 1436
MmeI TCCRAC 5 cut(s) 593, 722, 773, 789, 797
Mph1103I ATGCAT 2 cut(s) 1450, 1638
MseI TTAA 5 cut(s) 278, 420, 1163, 1695, 1908
MslI CAYNNNNRTG 6 cut(s) 108, 255, 429, 483, 865, 1733
MspI CCGG 1 cut(s) 1535
MspR9I CCNGG 4 cut(s) 20, 1044, 1374, 1509
Mva1269I GAATGC 3 cut(s) 1265, 1720, 1760
MvaI CCWGG 4 cut(s) 20, 1044, 1374, 1509
MvnI CGCG 1 cut(s) 1613
MwoI GCNNNNNNNGC 9 cut(s) 202, 205, 266, 341, 734, 1445, 1610, 1619, 1766
NcoI CCATGG 2 cut(s) 84, 1142
NdeII GATC 8 cut(s) 64, 133, 283, 457, 586, 1138, 1243, 1336
NlaIII CATG 8 cut(s) 88, 662, 737, 758, 1010, 1146, 1507, 1609
NlaIV GGNNCC 3 cut(s) 583, 712, 1774
NmuCI GTSAC 1 cut(s) 956
NsbI TGCGCA 1 cut(s) 357
NsiI ATGCAT 2 cut(s) 1450, 1638
NspI RCATGY 1 cut(s) 737
OliI CACNNNNGTG 2 cut(s) 255, 1733
PagI TCATGA 2 cut(s) 658, 754
PciSI GCTCTTC 1 cut(s) 987
PctI GAATGC 3 cut(s) 1265, 1720, 1760
PfeI GAWTC 4 cut(s) 175, 496, 1497, 1651
PflFI GACNNNGTC 2 cut(s) 164, 1374
Ple19I CGATCG 2 cut(s) 460, 589
PleI GAGTC 4 cut(s) 87, 619, 865, 1435
PpsI GAGTC 4 cut(s) 87, 619, 865, 1435
Ppu21I YACGTR 1 cut(s) 1887
PshBI ATTAAT 1 cut(s) 1695
PsiI TTATAA 1 cut(s) 825
Psp124BI GAGCTC 1 cut(s) 1029
Psp1406I AACGTT 1 cut(s) 814
Psp6I CCWGG 4 cut(s) 18, 1042, 1372, 1507
PspEI GGTNACC 2 cut(s) 1480, 1514
PspFI CCCAGC 1 cut(s) 488
PspGI CCWGG 4 cut(s) 18, 1042, 1372, 1507
PspN4I GGNNCC 3 cut(s) 583, 712, 1774
PspPI GGNCC 5 cut(s) 581, 710, 898, 1532, 1537
PstI CTGCAG 1 cut(s) 742
PsuI RGATCY 3 cut(s) 64, 1138, 1336
PsyI GACNNNGTC 2 cut(s) 164, 1374
PvuI CGATCG 2 cut(s) 460, 589
RsaI GTAC 3 cut(s) 147, 1326, 1722
RsaNI GTAC 3 cut(s) 146, 1325, 1721
RseI CAYNNNNRTG 6 cut(s) 108, 255, 429, 483, 865, 1733
SacI GAGCTC 1 cut(s) 1029
SapI GCTCTTC 1 cut(s) 987
SaqAI TTAA 5 cut(s) 278, 420, 1163, 1695, 1908
Sau3AI GATC 8 cut(s) 64, 133, 283, 457, 586, 1138, 1243, 1336
Sau96I GGNCC 5 cut(s) 581, 710, 898, 1532, 1537
ScaI AGTACT 1 cut(s) 147
SchI GAGTC 4 cut(s) 88, 619, 865, 1436
ScrFI CCNGG 4 cut(s) 20, 1044, 1374, 1509
SduI GDGCHC 4 cut(s) 786, 1029, 1730, 1775
SexAI ACCWGGT 1 cut(s) 1372
SfaNI GCATC 2 cut(s) 1428, 1645
SfcI CTRYAG 2 cut(s) 138, 738
SinI GGWCC 2 cut(s) 1532, 1537
SmiMI CAYNNNNRTG 6 cut(s) 108, 255, 429, 483, 865, 1733
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SsiI CCGC 5 cut(s) 228, 396, 1287, 1611, 1769
SspI AATATT 3 cut(s) 678, 1081, 1898
SspMI CTAG 3 cut(s) 779, 1560, 1880
SstI GAGCTC 1 cut(s) 1029
StyD4I CCNGG 4 cut(s) 18, 1042, 1372, 1507
StyI CCWWGG 6 cut(s) 84, 577, 706, 803, 1142, 1540
TaaI ACNGT 4 cut(s) 166, 504, 1480, 1725
TaiI ACGT 8 cut(s) 98, 259, 817, 943, 1158, 1350, 1817, 1889
TaqI TCGA 8 cut(s) 42, 77, 544, 592, 1132, 1381, 1649, 1670
TatI WGTACW 3 cut(s) 145, 1324, 1720
TfiI GAWTC 4 cut(s) 175, 496, 1497, 1651
Tru1I TTAA 5 cut(s) 278, 420, 1163, 1695, 1908
Tru9I TTAA 5 cut(s) 278, 420, 1163, 1695, 1908
TscAI CASTG 4 cut(s) 747, 887, 1234, 1624
TseFI GTSAC 1 cut(s) 956
Tsp45I GTSAC 1 cut(s) 956
TspDTI ATGAA 8 cut(s) 355, 546, 675, 771, 1034, 1225, 1428, 1785
TspGWI ACGGA 4 cut(s) 267, 860, 1322, 1615
TspRI CASTG 4 cut(s) 747, 887, 1234, 1624
Tth111I GACNNNGTC 2 cut(s) 164, 1374
VneI GTGCAC 1 cut(s) 1726
VpaK11BI GGWCC 2 cut(s) 1532, 1537
VspI ATTAAT 1 cut(s) 1695
XapI RAATTY 4 cut(s) 464, 1109, 1232, 1594
XceI RCATGY 1 cut(s) 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 713
XspI CTAG 3 cut(s) 779, 1560, 1880
ZrmI AGTACT 1 cut(s) 147
Zsp2I ATGCAT 2 cut(s) 1450, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.