MD02G1116900.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000489
Physical Location & Seq
Reverse (-)
9541225 .. 9542595
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1116900.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGATCGTGATATTTTCAATGCTGCAAAACAAGGCAATCTCAATGTCCTTAAGGAACATGGCGAGAATCTGGACAAAATATTGACTGCAACCAAGAACACAGTCCTTCATATCTATACAGCATGCTATAGGCGCCCAACGTTCAAAGAATCTGAGGACGAGATACTCAAGTCAACGAACACTGTGGACGGTATCCTTAAAATGTGTCCAGCATTACTATGGCAGCAGAACGATAGTGGTGATACTGCCCTACACATTGCGGCAAGATATGGGCGTGCTGGTATAGTTAGAGCTCTCCTCCTAGCTACAAAAACTTATTATCAAGGTGACCTTGAGCAAGGCTGTGCGATCTCATCAGAAGATGCCGCCTTGCAGGAGCAAAAACTCATCAGGAAAACCAACAACAAGAAGGACACAGCGCTGCACGAAGCTGTACGCTTTAATTACTTTGGTGTGGTGGAGGTATTAACCAAAGAAGACCCTGAGTTTTCCTACTCTCCTAATGATGCCGGCGAGACTCCTCTTTACCTAGCTGTCGAGAGGGGATACACTCATTATTGTCTTCAAATACTTGAAGGTTGCAAAAATCCAACTTATGAAGGCCCAAATGGTAGAACGGCTTTGTACGCTGCAATTATCCGCAATGATGAAGTAATGACGAGAGCATTACTAAAATCGGATAGAACTCTGGCAAAAGCAGCAGACGAACAAGGAGGCATTCCGCTTCACATGGCTGCAGATTTTGGTTACTTGTCAATGGTTAAGCAACTATTGGAATGTGATAAATCGACCGCCTACGTTGCTGACAATGATGGGCGCACAGCTCTTCACTTTGCAGCGTCCAAAGGCCATCTAGATATAATGAAAGAGCTTCTTACTCATTGCCCTGATTGTTGTGAACTGGTCGACAACCAATGTCAGAATGTGCTTCACTATGCCATAAAGAGCTATCAAGTTGACGTAGTTGATTTTGTGTTAACGGATCTGTGGCTTAGCAACATCCTCTTAAATGACAAGGATGCTAATGGCAATGCACCTCTTCATTACCTTGCTCATTATCCCAAACACCGCTACGCGGCTTATGATTCTATACTTGATGCTAAAATTGATATAATGTCATTCAACAAGAAAAATTTAAACGTTTTCGACACCATTCTAGCTAATGAGGACAACATAAGCAACGCACTTGAGCTGGTACGTATCACCTCTAATTTTCTTTTATGTTATTATGCAATTTTTCTTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

416

Amino Acids

46.61

Weight (kDa)

5.48

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 131 - 193 3.4e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 193 - 261 9.4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 242 - 326 2.7e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 242 - 289 1e-07 Ankyrin repeats (many copies)
Ank PF00023 271 - 297 2.7e-06 Ankyrin repeat
Ank_4 PF13637 276 - 324 3.3e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 132
AccI GTMKAC 1 cut(s) 906
AccII CGCG 1 cut(s) 1076
AciI CCGC 7 cut(s) 260, 366, 640, 722, 792, 1069, 1076
AclI AACGTT 2 cut(s) 140, 1140
AclWI GGATC 2 cut(s) 12, 990
AcsI RAATTY 1 cut(s) 1132
AcyI GRCGYC 1 cut(s) 133
AfaI GTAC 3 cut(s) 435, 626, 1197
AfeI AGCGCT 1 cut(s) 420
AfiI CCNNNNNNNGG 1 cut(s) 1075
AflII CTTAAG 1 cut(s) 50
AgsI TTSAA 6 cut(s) 19, 145, 566, 575, 1123, 1244
AluBI AGCT 9 cut(s) 293, 305, 431, 533, 824, 871, 948, 1160, 1192
AluI AGCT 9 cut(s) 293, 305, 431, 533, 824, 871, 948, 1160, 1192
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 1 cut(s) 509
AlwI GGATC 2 cut(s) 12, 990
Aor51HI AGCGCT 1 cut(s) 420
AoxI GGCC 2 cut(s) 601, 847
ApeKI GCWGC 7 cut(s) 23, 223, 421, 629, 698, 734, 836
ApoI RAATTY 1 cut(s) 1132
AspLEI GCGC 3 cut(s) 135, 421, 819
AspS9I GGNCC 1 cut(s) 602
AsuHPI GGTGA 3 cut(s) 251, 338, 1195
BaeI ACNNNNGTAYC 2 cut(s) 155, 188
BanI GGYRCC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 295
BbsI GAAGAC 2 cut(s) 483, 554
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 7 cut(s) 10, 235, 408, 616, 710, 721, 848
BccI CCATC 2 cut(s) 806, 858
BceAI ACGGC 1 cut(s) 633
BciVI GTATCC 2 cut(s) 203, 539
BcoDI GTCTC 1 cut(s) 509
BfaI CTAG 4 cut(s) 302, 530, 854, 1157
BfmI CTRYAG 2 cut(s) 127, 735
BfoI RGCGCY 2 cut(s) 136, 422
BfrI CTTAAG 1 cut(s) 50
BfuI GTATCC 2 cut(s) 203, 539
BlpI GCTNAGC 1 cut(s) 992
BmgT120I GGNCC 1 cut(s) 602
BmiI GGNNCC 1 cut(s) 134
BmsI GCATC 4 cut(s) 352, 496, 1009, 1087
BpiI GAAGAC 2 cut(s) 483, 554
BplI GAGNNNNNCTC 2 cut(s) 282, 314
Bpu1102I GCTNAGC 1 cut(s) 992
BpuEI CTTGAG 3 cut(s) 152, 353, 1208
BsaAI YACGTR 1 cut(s) 1199
BsaHI GRCGYC 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 1075
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 1 cut(s) 906
Bse3DI GCAATG 4 cut(s) 255, 649, 880, 1036
BseGI GGATG 2 cut(s) 999, 1024
BseLI CCNNNNNNNGG 1 cut(s) 1075
BseMI GCAATG 4 cut(s) 255, 649, 880, 1036
BseMII CTCAG 2 cut(s) 144, 474
BseNI ACTGG 1 cut(s) 906
BseRI GAGGAG 2 cut(s) 287, 510
BseXI GCAGC 7 cut(s) 10, 235, 408, 616, 710, 721, 848
BsgI GTGCAG 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 1076
Bsh1285I CGRYCG 1 cut(s) 792
BshFI GGCC 2 cut(s) 603, 849
BshNI GGYRCC 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 792
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 1075
BsmAI GTCTC 1 cut(s) 509
BsmI GAATGC 1 cut(s) 717
BsnI GGCC 2 cut(s) 603, 849
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 3 cut(s) 4, 348, 982
Bsp1720I GCTNAGC 1 cut(s) 992
BspACI CCGC 7 cut(s) 260, 366, 640, 722, 792, 1069, 1076
BspANI GGCC 2 cut(s) 603, 849
BspCNI CTCAG 2 cut(s) 145, 475
BspFNI CGCG 1 cut(s) 1076
BspLI GGNNCC 1 cut(s) 134
BspMAI CTGCAG 1 cut(s) 739
BspPI GGATC 2 cut(s) 12, 990
BspQI GCTCTTC 1 cut(s) 831
BspT107I GGYRCC 1 cut(s) 132
BspTI CTTAAG 1 cut(s) 50
BsrDI GCAATG 4 cut(s) 255, 649, 880, 1036
BsrFI RCCGGY 1 cut(s) 509
BsrI ACTGG 1 cut(s) 906
BssAI RCCGGY 1 cut(s) 509
BssMI GATC 3 cut(s) 4, 348, 982
BssNI GRCGYC 1 cut(s) 133
Bst4CI ACNGT 3 cut(s) 103, 184, 191
Bst6I CTCTTC 2 cut(s) 831, 1044
BstACI GRCGYC 1 cut(s) 133
BstAFI CTTAAG 1 cut(s) 50
BstBAI YACGTR 1 cut(s) 1199
BstC8I GCNNGC 3 cut(s) 124, 276, 511
BstDEI CTNAG 3 cut(s) 153, 483, 992
BstEII GGTNACC 1 cut(s) 326
BstF5I GGATG 2 cut(s) 999, 1024
BstFNI CGCG 1 cut(s) 1076
BstH2I RGCGCY 2 cut(s) 136, 422
BstHHI GCGC 3 cut(s) 135, 421, 819
BstKTI GATC 3 cut(s) 7, 351, 985
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 3 cut(s) 4, 348, 982
BstMCI CGRYCG 1 cut(s) 792
BstMWI GCNNNNNNNGC 4 cut(s) 132, 626, 698, 800
BstNSI RCATGY 1 cut(s) 126
BstPI GGTNACC 1 cut(s) 326
BstSFI CTRYAG 2 cut(s) 127, 735
BstSNI TACGTA 1 cut(s) 1199
BstUI CGCG 1 cut(s) 1076
BstV1I GCAGC 7 cut(s) 10, 235, 408, 616, 710, 721, 848
BstV2I GAAGAC 2 cut(s) 483, 554
BstX2I RGATCY 1 cut(s) 982
BstYI RGATCY 1 cut(s) 982
BsuI GTATCC 2 cut(s) 203, 539
BsuRI GGCC 2 cut(s) 603, 849
BtsCI GGATG 2 cut(s) 999, 1024
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 3 cut(s) 124, 276, 511
CfoI GCGC 3 cut(s) 135, 421, 819
Cfr10I RCCGGY 1 cut(s) 509
Cfr13I GGNCC 1 cut(s) 602
CseI GACGC 1 cut(s) 828
Csp6I GTAC 3 cut(s) 434, 625, 1196
CviAII CATG 3 cut(s) 59, 123, 730
CviQI GTAC 3 cut(s) 434, 625, 1196
DdeI CTNAG 3 cut(s) 153, 483, 992
DinI GGCGCC 1 cut(s) 134
DpnI GATC 3 cut(s) 6, 350, 984
DpnII GATC 3 cut(s) 4, 348, 982
DraI TTTAAA 1 cut(s) 1137
Eam1104I CTCTTC 2 cut(s) 831, 1044
EarI CTCTTC 2 cut(s) 831, 1044
Ecl136II GAGCTC 1 cut(s) 293
Eco105I TACGTA 1 cut(s) 1199
Eco24I GRGCYC 1 cut(s) 295
Eco47III AGCGCT 1 cut(s) 420
Eco53kI GAGCTC 1 cut(s) 293
Eco91I GGTNACC 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 326
EcoT38I GRGCYC 1 cut(s) 295
EgeI GGCGCC 1 cut(s) 134
EheI GGCGCC 1 cut(s) 134
FaeI CATG 3 cut(s) 62, 126, 733
FalI AAGNNNNNCTT 4 cut(s) 315, 347, 858, 890
FatI CATG 3 cut(s) 58, 122, 729
FblI GTMKAC 1 cut(s) 906
FokI GGATG 2 cut(s) 986, 1031
FriOI GRGCYC 1 cut(s) 295
FspBI CTAG 4 cut(s) 302, 530, 854, 1157
GlaI GCGC 3 cut(s) 134, 420, 818
HaeII RGCGCY 2 cut(s) 136, 422
HaeIII GGCC 2 cut(s) 603, 849
HapII CCGG 1 cut(s) 510
HgaI GACGC 1 cut(s) 828
HhaI GCGC 3 cut(s) 135, 421, 819
Hin1I GRCGYC 1 cut(s) 133
Hin1II CATG 3 cut(s) 62, 126, 733
Hin6I GCGC 3 cut(s) 133, 419, 817
HinP1I GCGC 3 cut(s) 133, 419, 817
HincII GTYRAC 4 cut(s) 174, 907, 958, 978
HindII GTYRAC 4 cut(s) 174, 907, 958, 978
HinfI GANTC 4 cut(s) 67, 149, 517, 1085
HpaI GTTAAC 1 cut(s) 978
HpaII CCGG 1 cut(s) 510
HphI GGTGA 3 cut(s) 251, 338, 1195
Hpy166II GTNNAC 6 cut(s) 174, 187, 899, 907, 958, 978
Hpy188I TCNGA 4 cut(s) 154, 358, 679, 921
Hpy188III TCNNGA 5 cut(s) 8, 71, 391, 538, 854
Hpy8I GTNNAC 6 cut(s) 174, 187, 899, 907, 958, 978
HpyAV CCTTC 4 cut(s) 116, 403, 569, 593
HpyCH4III ACNGT 3 cut(s) 103, 184, 191
HpyCH4IV ACGT 5 cut(s) 140, 798, 960, 1140, 1198
HpyF10VI GCNNNNNNNGC 4 cut(s) 132, 626, 698, 800
HpyF3I CTNAG 3 cut(s) 153, 483, 992
HpySE526I ACGT 5 cut(s) 140, 798, 960, 1140, 1198
Hsp92I GRCGYC 1 cut(s) 133
Hsp92II CATG 3 cut(s) 62, 126, 733
HspAI GCGC 3 cut(s) 133, 419, 817
KasI GGCGCC 1 cut(s) 132
KroI GCCGGC 1 cut(s) 509
KroNI GCCGGC 1 cut(s) 511
KspAI GTTAAC 1 cut(s) 978
Kzo9I GATC 3 cut(s) 4, 348, 982
LguI GCTCTTC 1 cut(s) 831
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 7 cut(s) 10, 235, 408, 616, 710, 721, 848
LweI GCATC 4 cut(s) 352, 496, 1009, 1087
MaeI CTAG 4 cut(s) 302, 530, 854, 1157
MaeII ACGT 5 cut(s) 140, 798, 960, 1140, 1198
MaeIII GTNAC 2 cut(s) 326, 746
MalI GATC 3 cut(s) 6, 350, 984
MboI GATC 3 cut(s) 4, 348, 982
MboII GAAGA 6 cut(s) 371, 488, 554, 818, 1031, 1232
MflI RGATCY 1 cut(s) 982
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 6 cut(s) 442, 633, 1104, 1132, 1210, 1233
Mly113I GGCGCC 1 cut(s) 133
MlyI GAGTC 1 cut(s) 511
MmeI TCCRAC 1 cut(s) 614
MroNI GCCGGC 1 cut(s) 509
MseI TTAA 8 cut(s) 51, 198, 441, 467, 762, 977, 1007, 1136
MslI CAYNNNNRTG 1 cut(s) 217
MspCI CTTAAG 1 cut(s) 50
MspI CCGG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 717
MvnI CGCG 1 cut(s) 1076
MwoI GCNNNNNNNGC 4 cut(s) 132, 626, 698, 800
NaeI GCCGGC 1 cut(s) 511
NarI GGCGCC 1 cut(s) 133
NdeII GATC 3 cut(s) 4, 348, 982
NgoMIV GCCGGC 1 cut(s) 509
NlaIII CATG 3 cut(s) 62, 126, 733
NlaIV GGNNCC 1 cut(s) 134
NmuCI GTSAC 1 cut(s) 326
NspI RCATGY 1 cut(s) 126
PaeI GCATGC 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 831
PctI GAATGC 1 cut(s) 717
PdiI GCCGGC 1 cut(s) 511
PfeI GAWTC 3 cut(s) 67, 149, 1085
PleI GAGTC 1 cut(s) 511
PluTI GGCGCC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 511
Ppu21I YACGTR 1 cut(s) 1199
Psp124BI GAGCTC 1 cut(s) 295
Psp1406I AACGTT 2 cut(s) 140, 1140
PspEI GGTNACC 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 134
PspPI GGNCC 1 cut(s) 602
PstI CTGCAG 1 cut(s) 739
PsuI RGATCY 1 cut(s) 982
RsaI GTAC 3 cut(s) 435, 626, 1197
RsaNI GTAC 3 cut(s) 434, 625, 1196
RseI CAYNNNNRTG 1 cut(s) 217
SacI GAGCTC 1 cut(s) 295
SalI GTCGAC 1 cut(s) 905
SapI GCTCTTC 1 cut(s) 831
SaqAI TTAA 8 cut(s) 51, 198, 441, 467, 762, 977, 1007, 1136
Sau3AI GATC 3 cut(s) 4, 348, 982
Sau96I GGNCC 1 cut(s) 602
SchI GAGTC 1 cut(s) 511
SduI GDGCHC 1 cut(s) 295
SfaNI GCATC 4 cut(s) 352, 496, 1009, 1087
SfcI CTRYAG 2 cut(s) 127, 735
SfoI GGCGCC 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 217
SmlI CTYRAG 4 cut(s) 50, 167, 332, 1187
SmoI CTYRAG 4 cut(s) 50, 167, 332, 1187
SnaBI TACGTA 1 cut(s) 1199
SphI GCATGC 1 cut(s) 126
Sse9I AATT 6 cut(s) 442, 633, 1104, 1132, 1210, 1233
SsiI CCGC 7 cut(s) 260, 366, 640, 722, 792, 1069, 1076
SspDI GGCGCC 1 cut(s) 132
SspI AATATT 1 cut(s) 81
SspMI CTAG 4 cut(s) 302, 530, 854, 1157
SstI GAGCTC 1 cut(s) 295
TaaI ACNGT 3 cut(s) 103, 184, 191
TaiI ACGT 5 cut(s) 143, 801, 963, 1143, 1201
TaqI TCGA 4 cut(s) 537, 788, 906, 1146
TasI AATT 6 cut(s) 442, 633, 1104, 1132, 1210, 1233
TauI GCSGC 3 cut(s) 263, 368, 1079
TfiI GAWTC 3 cut(s) 67, 149, 1085
Tru1I TTAA 8 cut(s) 51, 198, 441, 467, 762, 977, 1007, 1136
Tru9I TTAA 8 cut(s) 51, 198, 441, 467, 762, 977, 1007, 1136
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 326
TseI GCWGC 7 cut(s) 23, 223, 421, 629, 698, 734, 836
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 5 cut(s) 98, 612, 663, 878, 1031
TspGWI ACGGA 1 cut(s) 995
TspRI CASTG 1 cut(s) 187
Vha464I CTTAAG 1 cut(s) 50
XapI RAATTY 1 cut(s) 1132
XbaI TCTAGA 1 cut(s) 853
XceI RCATGY 1 cut(s) 126
XcmI CCANNNNNNNNNTGG 1 cut(s) 216
XmiI GTMKAC 1 cut(s) 906
XspI CTAG 4 cut(s) 302, 530, 854, 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.