MD15G1232200.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000489
Physical Location & Seq
Reverse (-)
18982544 .. 18985383
2840 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1232200.v1.1.491

Sequence Viewer

Length: 1860 bp
ATGGATGTTTCAAATCAGATCACCATCCAGGAAGAAGCAGCTGGTTCAAGTTTTATGGATCTTGGCGTGTACAATGCGGCAAAAGCCGGCGAAGTCGACGTCCTTTGGAAGCACAGAGACCACCTTGACCGAATGTTGACTCCAACCAAGAACACAATCCTCCATGTTTACATAGCATGCTCAACGTTGACTGAAACTGACTCCGAAGAAACACTACTACTAGTAATGAAGTCAGCTAATGTCGTCGAGAAGATTGTTGAAATGTGTCCGCCGTTACTGTTCCAGCCCAACGACGGCGGTGACACCGCATTGCACGTCGCGGCGAGGCACGGCTGTACTCGTGTGGTTCAAGTTCTTCTTAAGCAGGCTGCGAAAAGAGATTGCGGCCCCGTGGGAAAGCTGATGGGGGCAACCAACAAAGTGAAGGACACGGCGTTGCACGAGGCAGTGCGGTTTAACCACCGTGGTGTGGTGGAGGTGTTGACTCGAGAAGATCCTGAGGTTTTGTACTCTGCTAACGTAGACGGCGATACGCCGCTCTACTTGGCTGCTGAGAGGGGATACCGTGATTTGGTTTTCGAAATGCTTGGGACTTGTAAATGTTTAGCTTATGGAGGCCGCAACGGTAGAACGGCTTTGCACGCTGCTGTGATCTGCAACGATGAAGAAATGACGAGAGAATTACTAAAGTACACTGCAAAAATGGCTCTCACCAAAGCGGTAGATGGCCAAGGATGGACACCTCTTCACTTCGCTGCACTCTTCGGTCACCCTTCCATTGCGAAACAACTGCTAGAATCCGATAGATCGGCTGCGTACATTGGCGACAAAGAGTACAAGCAGACACCCCTCCACATTGCAGCCTTTGAAGGACATGAAGGTGTAATGAGAGAGCTTGTTTTGCATTGCCCCGATTGTTGTGAACTGGTCGACTACAAAGGCCGGAATGTTCTTCACTACGCCGTTAAGTGGTGTCAAAGTCCAATTGTACAGGATTTTGTTCTGAAGGATCCATGGGTTAGCAACACCTTGTTAAATGGAAAGGATGCTAATGGGAACACACCGCTCCATCTACTCGCTCTTTCTCCGCTATGCGCATCTGATCGTTTTTTATGTGATGGTAGAGTTGATAAGATGGCATTCAACAAGGCAAACCTAAATGCTTTAGACATTGTTCTGGCTGATGAAACTCTGCCACCAACACTCAAGAGTGCCAATGGATGCATTTTGGGAAGGAATGGGGCTAGACCAGGTTGTCATCGAATTTTATGCGGTGAAGACACTGTATGCCAAAAATTCTATCCTTTTAGAAAAATTGAAATTGAGCAATTCGAAGAAAACAAAGGTGGTAGCAGAAGTACAACATTCAATCTATTCGAATGTGGCAAAGACACAGATGTAATATTGAAGCAAGTCAAAGAAGCCCATCTAGTAATGGCTACACTGATTGCAACCGCAACCTTCGCAGCAGGTTTCGTCATGCCCGGTGTTTGTTACCAAAGTGAAAAAGGACCACCAAGCCAAGATTGTGCAGTTCTAACGAGACAAACAGCTTACCAAGCTTTTGTTGTAATGGATGCACTGGCCTTGTTTATGTCCACTTGTTCTGTCTTGATGCACTTTTATTTGTCTAGGCCGGACGCGATTGTTTGGGCGTTATCTTCTACCATGACCGCCTTGATTTTCACGGTGACTGCACTTATTACCGGCACATATGCAGTATCAGGTCATTCTCCAGGATTCGCCATCGCTGCTTGTGTGCTTGGCTTCTGGTACTTTTCCGCAGCTCTTTTCATGCTTGGTCGTCCATCTACCAAAGCTTTTCGGCGGCATTTTTTTCCAGTTGCCACCGCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

620

Amino Acids

67.9

Weight (kDa)

6.38

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 136 - 195 3.3e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 180 - 267 3.5e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 213 - 264 1.8e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 277 - 331 5e-07 Ankyrin repeats (3 copies)
PGG PF13962 469 - 574 3.9e-19 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000489)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10380 FvH4_1g10390 FvH4_1g10400 FvH4_1g10401 FvH4_1g10402 FvH4_1g10430 FvH4_1g10430 FvH4_1g12830 FvH4_1g12830 FvH4_1g12850 FvH4_1g12870
malus_domestica MD02G1014000.v1.1 MD02G1116900.v1.1 MD02G1117100.v1.1 MD07G1083500.v1.1 MD15G1231900.v1.1 MD15G1232000.v1.1 MD15G1232100.v1.1 MD15G1232200.v1.1
prunus_persica Prupe.7G182000_v2.0.a1 Prupe.7G182300_v2.0.a1 Prupe.7G182400_v2.0.a1 Prupe.7G182500_v2.0.a1 Prupe.7G182600_v2.0.a1
pyrus_communis pycom02g09070 pycom02g09080 pycom08g18250 pycom08g18260 pycom08g18270 pycom08g18280 pycom08g18290 pycom15g20630 pycom15g20650 pycom15g20660
rosa_chinensis RchiOBHm_Chr2g0097241 RchiOBHm_Chr7g0213401 RchiOBHm_Chr7g0237491
rosa_laevigata RLG00000000998 RLG00000002808 RLG00000016714
rosa_multiflora Rmu_co8242583.1_g000001 Rmu_sc0002425.1_g000003 Rmu_sc0002425.1_g000009 Rmu_sc0002923.1_g000001 Rmu_sc0036942.1_g000001 Rmu_ssc0000262.1_g000026 Rmu_ssc0000262.1_g000027
rosa_roxburghii Rroxscaffold_2G00144810 Rroxscaffold_2G00144850 Rroxscaffold_3G00224130 Rroxscaffold_3G00245990 Rroxscaffold_6G00396730
rosa_rugosa Rorug02G0054300 Rorug02G0055200 Rorug02G0065700 Rorug05G0283500 Rorug07G0303700
rosa_samantha Rh2AG112200 Rh2AG299500 Rh2BG114800 Rh2CG116800 Rh2CG117000 Rh2DG116000 Rh2DG116200 Rh2DG323600 Rh6AG214000 Rh6CG172700 Rh6DG163900 Rh7AG273800 Rh7AG461200 Rh7BG306800 Rh7BG307200 Rh7BG431000 Rh7BG431300 Rh7CG292900 Rh7CG478400 Rh7DG281200 Rh7DG323600 Rh7DG323700 Rh7DG447700
rosa_wichuraiana Rw0G001860 Rw1G008050 Rw1G011650 Rw2G008760 Rw2G008770 Rw3G028490 Rw6G018710 Rw7G023740 Rw7G038160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1254
AatII GACGTC 1 cut(s) 102
Acc16I TGCGCA 1 cut(s) 1096
Acc36I ACCTGC 1 cut(s) 1460
AccBSI CCGCTC 2 cut(s) 538, 1066
AccI GTMKAC 3 cut(s) 96, 522, 930
AccII CGCG 2 cut(s) 320, 1643
AclI AACGTT 1 cut(s) 185
AclWI GGATC 4 cut(s) 66, 488, 1004, 1017
AcoI YGGCCR 1 cut(s) 727
AcsI RAATTY 2 cut(s) 1263, 1295
AcuI CTGAAG 1 cut(s) 1025
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 9 cut(s) 71, 337, 509, 692, 818, 836, 990, 1360, 1775
AfiI CCNNNNNNNGG 4 cut(s) 293, 469, 571, 969
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 9 cut(s) 12, 48, 260, 350, 869, 1144, 1319, 1369, 1408
AhlI ACTAGT 1 cut(s) 220
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 3 cut(s) 27, 1249, 1735
AleI CACNNNNGTG 1 cut(s) 465
AluBI AGCT 9 cut(s) 41, 236, 400, 608, 895, 1553, 1562, 1787, 1820
AluI AGCT 9 cut(s) 41, 236, 400, 608, 895, 1553, 1562, 1787, 1820
Alw26I GTCTC 2 cut(s) 111, 1537
AlwI GGATC 4 cut(s) 66, 488, 1004, 1017
Ama87I CYCGRG 1 cut(s) 486
AoxI GGCC 6 cut(s) 385, 616, 727, 940, 1584, 1634
ApoI RAATTY 2 cut(s) 1263, 1295
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AspLEI GCGC 1 cut(s) 1097
AspS9I GGNCC 2 cut(s) 386, 1511
AsuC2I CCSGG 1 cut(s) 1485
AsuHPI GGTGA 6 cut(s) 13, 311, 703, 761, 1286, 1702
AsuII TTCGAA 3 cut(s) 579, 1332, 1377
AvaI CYCGRG 1 cut(s) 486
AvaII GGWCC 1 cut(s) 1511
AxyI CCTNAGG 1 cut(s) 498
BalI TGGCCA 1 cut(s) 729
BamHI GGATCC 1 cut(s) 1009
BarI GAAGNNNNNNTAC 2 cut(s) 198, 230
BauI CACGAG 2 cut(s) 339, 440
BbsI GAAGAC 1 cut(s) 1284
BceAI ACGGC 7 cut(s) 256, 310, 346, 447, 541, 648, 947
BcgI CGANNNNNNTGC 4 cut(s) 772, 806, 1251, 1285
BciT130I CCWGG 3 cut(s) 29, 1251, 1737
BciVI GTATCC 1 cut(s) 554
BcnI CCSGG 1 cut(s) 1485
BcoDI GTCTC 2 cut(s) 111, 1537
BcuI ACTAGT 1 cut(s) 220
BfaI CTAG 5 cut(s) 221, 794, 1245, 1430, 1632
BfrI CTTAAG 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 1460
BfuI GTATCC 1 cut(s) 554
Bme1390I CCNGG 4 cut(s) 29, 1251, 1485, 1737
Bme18I GGWCC 1 cut(s) 1511
BmeT110I CYCGRG 1 cut(s) 486
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 2 cut(s) 386, 1511
BmiI GGNNCC 2 cut(s) 388, 1011
BmrFI CCNGG 4 cut(s) 29, 1251, 1485, 1737
BmsI GCATC 5 cut(s) 1036, 1106, 1211, 1567, 1605
BpiI GAAGAC 1 cut(s) 1284
BpmI CTGGAG 1 cut(s) 1719
Bpu14I TTCGAA 3 cut(s) 579, 1332, 1377
BpuEI CTTGAG 1 cut(s) 1190
BpuMI CCSGG 1 cut(s) 1485
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 111
BsaJI CCNNGG 4 cut(s) 390, 463, 730, 1013
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 469, 571, 969
Bse118I RCCGGY 2 cut(s) 86, 1706
Bse1I ACTGG 3 cut(s) 930, 1587, 1841
Bse21I CCTNAGG 1 cut(s) 498
Bse3DI GCAATG 4 cut(s) 308, 777, 855, 904
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 3 cut(s) 29, 1251, 1737
BseDI CCNNGG 4 cut(s) 390, 463, 730, 1013
BseGI GGATG 6 cut(s) 10, 24, 740, 1051, 1226, 1582
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 4 cut(s) 293, 469, 571, 969
BseMI GCAATG 4 cut(s) 308, 777, 855, 904
BseMII CTCAG 2 cut(s) 489, 543
BseNI ACTGG 3 cut(s) 930, 1587, 1841
BsgI GTGCAG 3 cut(s) 741, 1551, 1680
Bsh1236I CGCG 2 cut(s) 320, 1643
BshFI GGCC 6 cut(s) 387, 618, 729, 942, 1586, 1636
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 5 cut(s) 87, 943, 1485, 1637, 1707
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 4 cut(s) 293, 469, 571, 969
BsmAI GTCTC 2 cut(s) 111, 1537
BsmFI GGGAC 1 cut(s) 604
BsmI GAATGC 1 cut(s) 1139
BsnI GGCC 6 cut(s) 387, 618, 729, 942, 1586, 1636
Bso31I GGTCTC 1 cut(s) 111
BsoBI CYCGRG 1 cut(s) 486
Bsp119I TTCGAA 3 cut(s) 579, 1332, 1377
Bsp1407I TGTACA 2 cut(s) 69, 988
Bsp143I GATC 7 cut(s) 18, 58, 493, 651, 806, 1009, 1102
Bsp19I CCATGG 1 cut(s) 1013
BspANI GGCC 6 cut(s) 387, 618, 729, 942, 1586, 1636
BspCNI CTCAG 2 cut(s) 490, 544
BspFNI CGCG 2 cut(s) 320, 1643
BspLI GGNNCC 2 cut(s) 388, 1011
BspMI ACCTGC 1 cut(s) 1460
BspPI GGATC 4 cut(s) 66, 488, 1004, 1017
BspT104I TTCGAA 3 cut(s) 579, 1332, 1377
BspTI CTTAAG 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 111
BsrBI CCGCTC 2 cut(s) 538, 1066
BsrDI GCAATG 4 cut(s) 308, 777, 855, 904
BsrFI RCCGGY 2 cut(s) 86, 1706
BsrGI TGTACA 2 cut(s) 69, 988
BsrI ACTGG 3 cut(s) 930, 1587, 1841
BssAI RCCGGY 2 cut(s) 86, 1706
BssECI CCNNGG 4 cut(s) 390, 463, 730, 1013
BssMI GATC 7 cut(s) 18, 58, 493, 651, 806, 1009, 1102
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 2 cut(s) 339, 440
BssT1I CCWWGG 2 cut(s) 730, 1013
Bst2BI CACGAG 2 cut(s) 339, 440
Bst2UI CCWGG 3 cut(s) 29, 1251, 1737
Bst4CI ACNGT 6 cut(s) 279, 464, 566, 626, 1285, 1690
Bst6I CTCTTC 2 cut(s) 750, 767
BstACI GRCGYC 1 cut(s) 99
BstAFI CTTAAG 1 cut(s) 359
BstAUI TGTACA 2 cut(s) 69, 988
BstBI TTCGAA 3 cut(s) 579, 1332, 1377
BstC8I GCNNGC 4 cut(s) 88, 178, 366, 642
BstDEI CTNAG 2 cut(s) 498, 552
BstDSI CCRYGG 3 cut(s) 390, 463, 1013
BstEII GGTNACC 1 cut(s) 767
BstF5I GGATG 6 cut(s) 10, 24, 740, 1051, 1226, 1582
BstFNI CGCG 2 cut(s) 320, 1643
BstHHI GCGC 1 cut(s) 1097
BstKTI GATC 7 cut(s) 21, 61, 496, 654, 809, 1012, 1105
BstMAI GTCTC 2 cut(s) 111, 1537
BstMBI GATC 7 cut(s) 18, 58, 493, 651, 806, 1009, 1102
BstMWI GCNNNNNNNGC 8 cut(s) 83, 641, 704, 901, 1463, 1559, 1751, 1853
BstNI CCWGG 3 cut(s) 29, 1251, 1737
BstNSI RCATGY 1 cut(s) 180
BstPI GGTNACC 1 cut(s) 767
BstSCI CCNGG 4 cut(s) 27, 1249, 1483, 1735
BstUI CGCG 2 cut(s) 320, 1643
BstV2I GAAGAC 1 cut(s) 1284
BstX2I RGATCY 3 cut(s) 58, 493, 1009
BstYI RGATCY 3 cut(s) 58, 493, 1009
Bsu36I CCTNAGG 1 cut(s) 498
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 6 cut(s) 387, 618, 729, 942, 1586, 1636
BtgI CCRYGG 3 cut(s) 390, 463, 1013
BtgZI GCGATG 1 cut(s) 1732
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 6 cut(s) 10, 24, 740, 1051, 1226, 1582
BtsI GCAGTG 2 cut(s) 453, 693
BtsIMutI CAGTG 5 cut(s) 453, 693, 1281, 1442, 1580
BveI ACCTGC 1 cut(s) 1460
Cac8I GCNNGC 4 cut(s) 88, 178, 366, 642
CfoI GCGC 1 cut(s) 1097
Cfr10I RCCGGY 2 cut(s) 86, 1706
Cfr13I GGNCC 2 cut(s) 386, 1511
CseI GACGC 1 cut(s) 1649
CsiI ACCWGGT 1 cut(s) 1249
Csp6I GTAC 9 cut(s) 70, 336, 508, 691, 817, 835, 989, 1359, 1774
CviAII CATG 7 cut(s) 164, 177, 875, 1014, 1480, 1669, 1795
CviQI GTAC 9 cut(s) 70, 336, 508, 691, 817, 835, 989, 1359, 1774
DdeI CTNAG 2 cut(s) 498, 552
DpnI GATC 7 cut(s) 20, 60, 495, 653, 808, 1011, 1104
DpnII GATC 7 cut(s) 18, 58, 493, 651, 806, 1009, 1102
DrdI GACNNNNNNGTC 1 cut(s) 1254
DseDI GACNNNNNNGTC 1 cut(s) 1254
EaeI YGGCCR 1 cut(s) 727
Eam1104I CTCTTC 2 cut(s) 750, 767
EarI CTCTTC 2 cut(s) 750, 767
EciI GGCGGA 1 cut(s) 258
Eco130I CCWWGG 2 cut(s) 730, 1013
Eco31I GGTCTC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 1511
Eco57I CTGAAG 1 cut(s) 1025
Eco81I CCTNAGG 1 cut(s) 498
Eco88I CYCGRG 1 cut(s) 486
Eco91I GGTNACC 1 cut(s) 767
EcoO65I GGTNACC 1 cut(s) 767
EcoRII CCWGG 3 cut(s) 27, 1249, 1735
EcoT14I CCWWGG 2 cut(s) 730, 1013
EcoT22I ATGCAT 1 cut(s) 1226
ErhI CCWWGG 2 cut(s) 730, 1013
FaeI CATG 7 cut(s) 167, 180, 878, 1017, 1483, 1672, 1798
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FaqI GGGAC 1 cut(s) 604
FatI CATG 7 cut(s) 163, 176, 874, 1013, 1479, 1668, 1794
FauNDI CATATG 1 cut(s) 1714
FblI GTMKAC 3 cut(s) 96, 522, 930
FokI GGATG 6 cut(s) 11, 17, 747, 1058, 1233, 1589
FspAI RTGCGCAY 1 cut(s) 1096
FspBI CTAG 5 cut(s) 221, 794, 1245, 1430, 1632
FspI TGCGCA 1 cut(s) 1096
GlaI GCGC 1 cut(s) 1096
GsuI CTGGAG 1 cut(s) 1719
HaeIII GGCC 6 cut(s) 387, 618, 729, 942, 1586, 1636
HapII CCGG 5 cut(s) 87, 943, 1485, 1637, 1707
HgaI GACGC 1 cut(s) 1649
HhaI GCGC 1 cut(s) 1097
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 7 cut(s) 167, 180, 878, 1017, 1483, 1672, 1798
Hin6I GCGC 1 cut(s) 1095
HinP1I GCGC 1 cut(s) 1095
HincII GTYRAC 5 cut(s) 97, 138, 189, 483, 931
HindII GTYRAC 5 cut(s) 97, 138, 189, 483, 931
HindIII AAGCTT 2 cut(s) 1560, 1818
HinfI GANTC 5 cut(s) 139, 200, 484, 797, 1740
HpaII CCGG 5 cut(s) 87, 943, 1485, 1637, 1707
HphI GGTGA 6 cut(s) 13, 311, 703, 761, 1286, 1702
Hpy188I TCNGA 5 cut(s) 18, 205, 802, 1005, 1102
Hpy188III TCNNGA 5 cut(s) 247, 488, 497, 1207, 1612
Hpy99I CGWCG 4 cut(s) 101, 248, 296, 320
HpyAV CCTTC 7 cut(s) 418, 783, 863, 872, 1000, 1227, 1471
HpyCH4III ACNGT 6 cut(s) 279, 464, 566, 626, 1285, 1690
HpyCH4IV ACGT 4 cut(s) 99, 185, 315, 519
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 641, 704, 901, 1463, 1559, 1751, 1853
HpyF3I CTNAG 2 cut(s) 498, 552
HpySE526I ACGT 4 cut(s) 99, 185, 315, 519
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 7 cut(s) 167, 180, 878, 1017, 1483, 1672, 1798
HspAI GCGC 1 cut(s) 1095
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 7 cut(s) 18, 58, 493, 651, 806, 1009, 1102
LmnI GCTCC 1 cut(s) 1071
LweI GCATC 5 cut(s) 1036, 1106, 1211, 1567, 1605
MabI ACCWGGT 1 cut(s) 1249
MaeI CTAG 5 cut(s) 221, 794, 1245, 1430, 1632
MaeII ACGT 4 cut(s) 99, 185, 315, 519
MaeIII GTNAC 5 cut(s) 273, 299, 767, 1493, 1690
MalI GATC 7 cut(s) 20, 60, 495, 653, 808, 1011, 1104
MbiI CCGCTC 2 cut(s) 538, 1066
MboI GATC 7 cut(s) 18, 58, 493, 651, 806, 1009, 1102
MfeI CAATTG 1 cut(s) 984
MflI RGATCY 3 cut(s) 58, 493, 1009
MlsI TGGCCA 1 cut(s) 729
MluCI AATT 7 cut(s) 680, 984, 1263, 1295, 1314, 1320, 1328
MluNI TGGCCA 1 cut(s) 729
MlyI GAGTC 3 cut(s) 133, 194, 478
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 9 cut(s) 170, 318, 436, 469, 493, 549, 608, 753, 860
Mox20I TGGCCA 1 cut(s) 729
Mph1103I ATGCAT 1 cut(s) 1226
MroNI GCCGGC 1 cut(s) 86
MscI TGGCCA 1 cut(s) 729
MseI TTAA 4 cut(s) 360, 456, 966, 1034
MslI CAYNNNNRTG 2 cut(s) 465, 879
Msp20I TGGCCA 1 cut(s) 729
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 5 cut(s) 87, 943, 1485, 1637, 1707
MspR9I CCNGG 4 cut(s) 29, 1251, 1485, 1737
MunI CAATTG 1 cut(s) 984
Mva1269I GAATGC 1 cut(s) 1139
MvaI CCWGG 3 cut(s) 29, 1251, 1737
MvnI CGCG 2 cut(s) 320, 1643
MwoI GCNNNNNNNGC 8 cut(s) 83, 641, 704, 901, 1463, 1559, 1751, 1853
NaeI GCCGGC 1 cut(s) 88
NciI CCSGG 1 cut(s) 1485
NcoI CCATGG 1 cut(s) 1013
NdeI CATATG 1 cut(s) 1714
NdeII GATC 7 cut(s) 18, 58, 493, 651, 806, 1009, 1102
NgoMIV GCCGGC 1 cut(s) 86
NlaIII CATG 7 cut(s) 167, 180, 878, 1017, 1483, 1672, 1798
NlaIV GGNNCC 2 cut(s) 388, 1011
NmuCI GTSAC 3 cut(s) 299, 767, 1690
NsbI TGCGCA 1 cut(s) 1096
NsiI ATGCAT 1 cut(s) 1226
NspI RCATGY 1 cut(s) 180
NspV TTCGAA 3 cut(s) 579, 1332, 1377
OliI CACNNNNGTG 1 cut(s) 465
PaeI GCATGC 1 cut(s) 180
PaeR7I CTCGAG 1 cut(s) 486
PcsI WCGNNNNNNNCGW 1 cut(s) 525
PctI GAATGC 1 cut(s) 1139
PdiI GCCGGC 1 cut(s) 88
PfeI GAWTC 2 cut(s) 797, 1740
PfoI TCCNGGA 2 cut(s) 27, 1735
PleI GAGTC 3 cut(s) 133, 194, 478
PpsI GAGTC 3 cut(s) 133, 194, 478
Psp1406I AACGTT 1 cut(s) 185
Psp6I CCWGG 3 cut(s) 27, 1249, 1735
PspEI GGTNACC 1 cut(s) 767
PspGI CCWGG 3 cut(s) 27, 1249, 1735
PspN4I GGNNCC 2 cut(s) 388, 1011
PspPI GGNCC 2 cut(s) 386, 1511
PsuI RGATCY 3 cut(s) 58, 493, 1009
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 9 cut(s) 71, 337, 509, 692, 818, 836, 990, 1360, 1775
RsaNI GTAC 9 cut(s) 70, 336, 508, 691, 817, 835, 989, 1359, 1774
RseI CAYNNNNRTG 2 cut(s) 465, 879
SalI GTCGAC 2 cut(s) 95, 929
SaqAI TTAA 4 cut(s) 360, 456, 966, 1034
Sau3AI GATC 7 cut(s) 18, 58, 493, 651, 806, 1009, 1102
Sau96I GGNCC 2 cut(s) 386, 1511
SchI GAGTC 3 cut(s) 133, 194, 478
ScrFI CCNGG 4 cut(s) 29, 1251, 1485, 1737
SexAI ACCWGGT 1 cut(s) 1249
SfaNI GCATC 5 cut(s) 1036, 1106, 1211, 1567, 1605
Sfr274I CTCGAG 1 cut(s) 486
SfuI TTCGAA 3 cut(s) 579, 1332, 1377
SinI GGWCC 1 cut(s) 1511
SlaI CTCGAG 1 cut(s) 486
SmiMI CAYNNNNRTG 2 cut(s) 465, 879
SmlI CTYRAG 3 cut(s) 359, 486, 1205
SmoI CTYRAG 3 cut(s) 359, 486, 1205
SpeI ACTAGT 1 cut(s) 220
SphI GCATGC 1 cut(s) 180
Sse9I AATT 7 cut(s) 680, 984, 1263, 1295, 1314, 1320, 1328
SspI AATATT 1 cut(s) 1404
SspMI CTAG 5 cut(s) 221, 794, 1245, 1430, 1632
StyD4I CCNGG 4 cut(s) 27, 1249, 1483, 1735
StyI CCWWGG 2 cut(s) 730, 1013
TaaI ACNGT 6 cut(s) 279, 464, 566, 626, 1285, 1690
TaiI ACGT 4 cut(s) 102, 188, 318, 522
TaqI TCGA 8 cut(s) 96, 246, 487, 579, 930, 1261, 1332, 1377
TaqII GACCGA 2 cut(s) 144, 755
TasI AATT 7 cut(s) 680, 984, 1263, 1295, 1314, 1320, 1328
TatI WGTACW 7 cut(s) 69, 335, 507, 690, 834, 988, 1358
TauI GCSGC 6 cut(s) 80, 323, 387, 538, 621, 1831
TfiI GAWTC 2 cut(s) 797, 1740
Tru1I TTAA 4 cut(s) 360, 456, 966, 1034
Tru9I TTAA 4 cut(s) 360, 456, 966, 1034
TscAI CASTG 5 cut(s) 453, 700, 1288, 1449, 1587
TseFI GTSAC 3 cut(s) 299, 767, 1690
Tsp45I GTSAC 3 cut(s) 299, 767, 1690
TspDTI ATGAA 5 cut(s) 242, 678, 891, 1200, 1783
TspRI CASTG 5 cut(s) 453, 700, 1288, 1449, 1587
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 1511
XapI RAATTY 2 cut(s) 1263, 1295
XceI RCATGY 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 486
XmiI GTMKAC 3 cut(s) 96, 522, 930
XspI CTAG 5 cut(s) 221, 794, 1245, 1430, 1632
ZraI GACGTC 1 cut(s) 100
Zsp2I ATGCAT 1 cut(s) 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.