FvH4_2g06640

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000508
Physical Location & Seq
Forward (+)
5512967 .. 5515122
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06640.t1

Sequence Viewer

Length: 633 bp
ATGGGCAAAGAAATAGTTCAACAAGAGTCACTATTGAAGCTTGAACGTCCTAGGAGAGTATGGTTCAATGATTTTAACCATATTCTACTGGAGAACACTGATAGAAATAGACTGAATTTACTGAAGAAAGACATTGTGATCAAGATGCCCAGACCAAATGATTTATACTTGACTGCTAAAAGTTTCTCAAAGGCTACTTCAGCAGATTGGACCTATTTGCTCGTTGGTCTCATCCTAGAGATTTCTTCCGTGTTCTTTGATCAGGAATCATCTCCAAGTAAGCCCTTGAATGCTTTCTTTGGTATGCTCTTAGCCATTGCATCTCTACTCACTTGCATATGTGAGCTCATTCACAAGGGTATAAAGGAAAGAGTTGTACTGAGGCGTTGTGGCACGTTATGGTGGTTTCGTTATCCCAATTCTCGTTATGTACTATTTGGTAACCTTCGTGATTGCTTCGGATTACTTGGTAGCATCTTTCAGTGCATTTGCTCGACGATTCAATATGTTTACTACATTCGCTGTGGTACAAATCCAATCAAGGTCTCCCTTTTGCCTGCCATCTTTCTTATATGTGTGGCGCTGTTGAATTCAAGTACTCGTAAGGATGAGATCATTGAACAAAATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

211

Amino Acids

24.26

Weight (kDa)

9.03

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 115, 589
AcuI CTGAAG 2 cut(s) 143, 183
AfaI GTAC 4 cut(s) 378, 432, 529, 598
AgsI TTSAA 9 cut(s) 20, 37, 44, 67, 289, 503, 589, 594, 620
AluBI AGCT 2 cut(s) 40, 346
AluI AGCT 2 cut(s) 40, 346
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 2 cut(s) 233, 550
ApoI RAATTY 2 cut(s) 115, 589
Asp700I GAANNNNTTC 2 cut(s) 15, 293
AspA2I CCTAGG 1 cut(s) 50
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 1 cut(s) 210
AvaII GGWCC 1 cut(s) 210
AvrII CCTAGG 1 cut(s) 50
BanII GRGCYC 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 348
BccI CCATC 1 cut(s) 569
BclI TGATCA 2 cut(s) 138, 259
BcoDI GTCTC 2 cut(s) 233, 550
BfaI CTAG 2 cut(s) 51, 236
BfoI RGCGCY 1 cut(s) 584
BlnI CCTAGG 1 cut(s) 50
BmcAI AGTACT 1 cut(s) 598
Bme18I GGWCC 1 cut(s) 210
BmgT120I GGNCC 1 cut(s) 210
BmsI GCATC 3 cut(s) 135, 329, 483
BpmI CTGGAG 1 cut(s) 110
BsaI GGTCTC 2 cut(s) 233, 550
BsaJI CCNNGG 1 cut(s) 50
Bse1I ACTGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 2 cut(s) 231, 613
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 348
BsmAI GTCTC 2 cut(s) 233, 550
BsmI GAATGC 1 cut(s) 295
Bso31I GGTCTC 2 cut(s) 233, 550
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 3 cut(s) 138, 259, 612
BspCNI CTCAG 1 cut(s) 372
BspTNI GGTCTC 2 cut(s) 233, 550
BsrDI GCAATG 1 cut(s) 315
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 3 cut(s) 138, 259, 612
BssT1I CCWWGG 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 2 cut(s) 310, 380
BstEII GGTNACC 1 cut(s) 440
BstF5I GGATG 2 cut(s) 231, 613
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 3 cut(s) 141, 262, 615
BstMAI GTCTC 2 cut(s) 233, 550
BstMBI GATC 3 cut(s) 138, 259, 612
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstPI GGTNACC 1 cut(s) 440
BtsCI GGATG 2 cut(s) 231, 613
BtsIMutI CAGTG 2 cut(s) 96, 488
Cac8I GCNNGC 1 cut(s) 558
CfoI GCGC 1 cut(s) 583
Cfr13I GGNCC 1 cut(s) 210
Csp6I GTAC 4 cut(s) 377, 431, 528, 597
CviJI RGCY 5 cut(s) 40, 194, 283, 314, 346
CviKI_1 RGCY 5 cut(s) 40, 194, 283, 314, 346
CviQI GTAC 4 cut(s) 377, 431, 528, 597
DdeI CTNAG 2 cut(s) 310, 380
DpnI GATC 3 cut(s) 140, 261, 614
DpnII GATC 3 cut(s) 138, 259, 612
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 348
Eco31I GGTCTC 2 cut(s) 233, 550
Eco47I GGWCC 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 346
Eco57I CTGAAG 2 cut(s) 143, 183
Eco91I GGTNACC 1 cut(s) 440
EcoICRI GAGCTC 1 cut(s) 346
EcoO65I GGTNACC 1 cut(s) 440
EcoRI GAATTC 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 50
FauNDI CATATG 1 cut(s) 338
FbaI TGATCA 2 cut(s) 138, 259
FokI GGATG 2 cut(s) 218, 620
FriOI GRGCYC 1 cut(s) 348
FspBI CTAG 2 cut(s) 51, 236
GlaI GCGC 1 cut(s) 582
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 584
HhaI GCGC 1 cut(s) 583
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 3 cut(s) 26, 266, 499
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 1 cut(s) 461
Hpy188III TCNNGA 3 cut(s) 142, 263, 449
Hpy8I GTNNAC 1 cut(s) 511
Hpy99I CGWCG 1 cut(s) 499
HpyAV CCTTC 1 cut(s) 455
HpyCH4IV ACGT 2 cut(s) 46, 395
HpyCH4V TGCA 3 cut(s) 320, 336, 486
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 2 cut(s) 310, 380
HpySE526I ACGT 2 cut(s) 46, 395
HspAI GCGC 1 cut(s) 581
Ksp22I TGATCA 2 cut(s) 138, 259
Kzo9I GATC 3 cut(s) 138, 259, 612
LpnPI CCDG 4 cut(s) 74, 163, 248, 570
LweI GCATC 3 cut(s) 135, 329, 483
MaeI CTAG 2 cut(s) 51, 236
MaeII ACGT 2 cut(s) 46, 395
MaeIII GTNAC 2 cut(s) 27, 440
MalI GATC 3 cut(s) 140, 261, 614
MboI GATC 3 cut(s) 138, 259, 612
MboII GAAGA 2 cut(s) 136, 237
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 3 cut(s) 115, 418, 589
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 1 cut(s) 375
MroXI GAANNNNTTC 2 cut(s) 15, 293
MseI TTAA 1 cut(s) 75
Mva1269I GAATGC 1 cut(s) 295
MwoI GCNNNNNNNGC 1 cut(s) 200
NdeI CATATG 1 cut(s) 338
NdeII GATC 3 cut(s) 138, 259, 612
NmuCI GTSAC 1 cut(s) 27
PctI GAATGC 1 cut(s) 295
PdmI GAANNNNTTC 2 cut(s) 15, 293
PfeI GAWTC 2 cut(s) 266, 499
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Psp124BI GAGCTC 1 cut(s) 348
PspEI GGTNACC 1 cut(s) 440
PspPI GGNCC 1 cut(s) 210
RsaI GTAC 4 cut(s) 378, 432, 529, 598
RsaNI GTAC 4 cut(s) 377, 431, 528, 597
SacI GAGCTC 1 cut(s) 348
SaqAI TTAA 1 cut(s) 75
Sau3AI GATC 3 cut(s) 138, 259, 612
Sau96I GGNCC 1 cut(s) 210
ScaI AGTACT 1 cut(s) 598
SchI GAGTC 1 cut(s) 35
SduI GDGCHC 1 cut(s) 348
SetI ASST 7 cut(s) 42, 49, 215, 348, 398, 447, 546
SfaNI GCATC 3 cut(s) 135, 329, 483
SinI GGWCC 1 cut(s) 210
Sse9I AATT 3 cut(s) 115, 418, 589
SspMI CTAG 2 cut(s) 51, 236
SstI GAGCTC 1 cut(s) 348
StyI CCWWGG 1 cut(s) 50
TaiI ACGT 2 cut(s) 49, 398
TaqI TCGA 1 cut(s) 494
TasI AATT 3 cut(s) 115, 418, 589
TatI WGTACW 3 cut(s) 376, 430, 596
TfiI GAWTC 2 cut(s) 266, 499
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 2 cut(s) 103, 488
TseFI GTSAC 1 cut(s) 27
Tsp45I GTSAC 1 cut(s) 27
TspGWI ACGGA 1 cut(s) 238
TspRI CASTG 2 cut(s) 103, 488
VpaK11BI GGWCC 1 cut(s) 210
XapI RAATTY 2 cut(s) 115, 589
XmaJI CCTAGG 1 cut(s) 50
XmnI GAANNNNTTC 2 cut(s) 15, 293
XspI CTAG 2 cut(s) 51, 236
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.