FvH4_2g36090

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000508
Physical Location & Seq
Forward (+)
26494000 .. 26496599
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36090.t2

Sequence Viewer

Length: 1068 bp
ATGGTTTTGAAGAAGCGTAGAGTATCAATTCCATTTCCATTACGGATATCTATTCCTTCAAATTCTATATGGGCGGTTGATGTTTTCTTAAGTTGTGACCATGAAGACATTTTCTGCTCCCACTTGTACCTCAAGTTGCAGTCTCGAGGAATTTGTACTTTCCAGTTCTTTGATGCAATCGAAGAATCGAGGTTTGCCATTGTTGTTCTCTCGCAAAACTATGCTTCTTCTCCACGGCGCTTGAATGAGCTTTCCAAAATTCTCGAGCGCATGAAAGATAGGAACAGGATTCTACCGATATTTCATGATGTGAATCTCTTCCATGTCCAAAAACAAAAAGGGACTTTTGAGAAAGCATTTGCCAAGCATGAAGAAATGTTTCAGGATGACTTGGCAAAGGTTGAAGCTTGGAGAGATGCTTTAACCAAAGTATGCAACTTTGCTGGTTGGACTTCCAATGATAGAAATGAAGTACAAGTTCTAGAAGAAATTACTGAAGCAGTCTGGAACAAAATACATCCTAGACCAAGACCAACCGAAAGAGTGCATCCGACATCCACACACAACTTGGTTGCACACACTAGCATTAAGGTTAATGAGGGCAACCGTAACCATCCAATGCACACTACAGATTTGGTGGATACTTCAGCAGAGTGGAACTTTTTGATCGCTAATCTTGGTCTAGAGATTTTGTCAGCTGGTTTTGATCAGGCCTCCTCCTCAAGTAAGCCACAGTATGCACTATTTGGTATGCTGTTTTCTATTGCAGCCTTGTTCACTTGCATTTTGGAGCTCATTTACAAGGGCATAAATAATAGAGTAGTACTGAAGAAGTTCGGAAAACTCTGGTGGTATTATTATCCACATCCCCACGATAGCGTGCCTTTTGGTACTCTCCCTGACATTTACGGATTATTTGGAGGTGTTGCTCAGTGCATATGCTCTATTACTCAATACATTTACTTCTCTTGGCATGCTGATAGTCCTATAAAACTATCACTTTTACCAGCAATCTTTCTTTTTTGTTTGGCTGGTTCAAGACTACACAGCAATCGTAATCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

356

Amino Acids

40.88

Weight (kDa)

8.54

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR_2 PF13676 29 - 104 4.9e-06 TIR domain
TIR PF01582 32 - 175 2e-27 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 74
AcsI RAATTY 3 cut(s) 61, 150, 258
AcuI CTGAAG 3 cut(s) 516, 630, 848
AfaI GTAC 5 cut(s) 128, 157, 474, 825, 892
AflII CTTAAG 1 cut(s) 88
AgsI TTSAA 5 cut(s) 10, 60, 244, 404, 1038
AluBI AGCT 4 cut(s) 250, 407, 698, 793
AluI AGCT 4 cut(s) 250, 407, 698, 793
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 1 cut(s) 147
Ama87I CYCGRG 2 cut(s) 144, 263
AoxI GGCC 1 cut(s) 711
ApeKI GCWGC 1 cut(s) 767
ApoI RAATTY 3 cut(s) 61, 150, 258
Asp700I GAANNNNTTC 3 cut(s) 317, 378, 833
AspLEI GCGC 2 cut(s) 240, 270
AvaI CYCGRG 2 cut(s) 144, 263
BanII GRGCYC 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 795
BbvI GCAGC 1 cut(s) 779
BccI CCATC 1 cut(s) 621
BceAI ACGGC 1 cut(s) 251
BciVI GTATCC 1 cut(s) 634
BclI TGATCA 1 cut(s) 706
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 4 cut(s) 482, 522, 582, 683
BfmI CTRYAG 1 cut(s) 627
BfoI RGCGCY 1 cut(s) 241
BfrI CTTAAG 1 cut(s) 88
BfuI GTATCC 1 cut(s) 634
BisI GCNGC 1 cut(s) 768
BlsI GCNGC 1 cut(s) 769
BmcAI AGTACT 1 cut(s) 825
BmeT110I CYCGRG 2 cut(s) 144, 263
BmsI GCATC 3 cut(s) 163, 406, 556
BpiI GAAGAC 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 116, 706
BsaBI GATNNNNATC 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 233
Bse1I ACTGG 1 cut(s) 163
Bse8I GATNNNNATC 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 233
BseGI GGATG 6 cut(s) 391, 517, 547, 554, 613, 865
BseJI GATNNNNATC 1 cut(s) 312
BseMII CTCAG 1 cut(s) 944
BseNI ACTGG 1 cut(s) 163
BseRI GAGGAG 2 cut(s) 706, 709
BseXI GCAGC 1 cut(s) 779
BshFI GGCC 1 cut(s) 713
BsiHKAI GWGCWC 1 cut(s) 795
BsiHKCI CYCGRG 2 cut(s) 144, 263
BslFI GGGAC 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 355
BsnI GGCC 1 cut(s) 713
BsoBI CYCGRG 2 cut(s) 144, 263
Bsp1286I GDGCHC 1 cut(s) 795
Bsp143I GATC 2 cut(s) 666, 706
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 713
BspCNI CTCAG 1 cut(s) 943
BspHI TCATGA 1 cut(s) 304
BspTI CTTAAG 1 cut(s) 88
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 233
BssMI GATC 2 cut(s) 666, 706
Bst4CI ACNGT 2 cut(s) 608, 735
Bst6I CTCTTC 1 cut(s) 323
BstAFI CTTAAG 1 cut(s) 88
BstC8I GCNNGC 2 cut(s) 881, 975
BstDEI CTNAG 1 cut(s) 930
BstDSI CCRYGG 1 cut(s) 233
BstF5I GGATG 6 cut(s) 391, 517, 547, 554, 613, 865
BstH2I RGCGCY 1 cut(s) 241
BstHHI GCGC 2 cut(s) 240, 270
BstKTI GATC 2 cut(s) 669, 709
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 2 cut(s) 666, 706
BstNSI RCATGY 1 cut(s) 977
BstSFI CTRYAG 1 cut(s) 627
BstV1I GCAGC 1 cut(s) 779
BstV2I GAAGAC 1 cut(s) 111
BsuI GTATCC 1 cut(s) 634
BsuRI GGCC 1 cut(s) 713
BtgI CCRYGG 1 cut(s) 233
BtsCI GGATG 6 cut(s) 391, 517, 547, 554, 613, 865
BtsIMutI CAGTG 1 cut(s) 938
Cac8I GCNNGC 2 cut(s) 881, 975
CciI TCATGA 1 cut(s) 304
CfoI GCGC 2 cut(s) 240, 270
Csp6I GTAC 5 cut(s) 127, 156, 473, 824, 891
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviAII CATG 6 cut(s) 101, 271, 305, 323, 368, 974
CviJI RGCY 8 cut(s) 250, 407, 698, 713, 730, 770, 793, 1031
CviKI_1 RGCY 8 cut(s) 250, 407, 698, 713, 730, 770, 793, 1031
CviQI GTAC 5 cut(s) 127, 156, 473, 824, 891
DdeI CTNAG 1 cut(s) 930
DpnI GATC 2 cut(s) 668, 708
DpnII GATC 2 cut(s) 666, 706
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
Ecl136II GAGCTC 1 cut(s) 793
Eco147I AGGCCT 1 cut(s) 713
Eco24I GRGCYC 1 cut(s) 795
Eco32I GATATC 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 793
Eco57I CTGAAG 3 cut(s) 516, 630, 848
Eco88I CYCGRG 2 cut(s) 144, 263
EcoICRI GAGCTC 1 cut(s) 793
EcoRV GATATC 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 795
FaeI CATG 6 cut(s) 104, 274, 308, 326, 371, 977
FaqI GGGAC 1 cut(s) 355
FatI CATG 6 cut(s) 100, 270, 304, 322, 367, 973
FauNDI CATATG 1 cut(s) 938
FbaI TGATCA 1 cut(s) 706
Fnu4HI GCNGC 1 cut(s) 768
FokI GGATG 6 cut(s) 398, 504, 534, 541, 600, 852
FriOI GRGCYC 1 cut(s) 795
Fsp4HI GCNGC 1 cut(s) 768
FspBI CTAG 4 cut(s) 482, 522, 582, 683
GlaI GCGC 2 cut(s) 239, 269
GluI GCNGC 1 cut(s) 768
HaeII RGCGCY 1 cut(s) 241
HaeIII GGCC 1 cut(s) 713
HhaI GCGC 2 cut(s) 240, 270
Hin1II CATG 6 cut(s) 104, 274, 308, 326, 371, 977
Hin6I GCGC 2 cut(s) 238, 268
HinP1I GCGC 2 cut(s) 238, 268
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 3 cut(s) 185, 289, 313
Hpy166II GTNNAC 1 cut(s) 777
Hpy188I TCNGA 2 cut(s) 552, 839
Hpy188III TCNNGA 8 cut(s) 144, 263, 305, 383, 482, 505, 683, 1038
Hpy8I GTNNAC 1 cut(s) 777
HpyAV CCTTC 1 cut(s) 66
HpyCH4III ACNGT 2 cut(s) 608, 735
HpyF3I CTNAG 1 cut(s) 930
Hsp92II CATG 6 cut(s) 104, 274, 308, 326, 371, 977
HspAI GCGC 2 cut(s) 238, 268
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 2 cut(s) 666, 706
LmnI GCTCC 2 cut(s) 122, 790
Lsp1109I GCAGC 1 cut(s) 779
LweI GCATC 3 cut(s) 163, 406, 556
MaeI CTAG 4 cut(s) 482, 522, 582, 683
MaeIII GTNAC 2 cut(s) 95, 608
MalI GATC 2 cut(s) 668, 708
MboI GATC 2 cut(s) 666, 706
MboII GAAGA 8 cut(s) 22, 116, 194, 219, 310, 383, 497, 841
MhlI GDGCHC 1 cut(s) 795
MluCI AATT 5 cut(s) 27, 61, 150, 258, 489
MmeI TCCRAC 2 cut(s) 428, 575
MnlI CCTC 8 cut(s) 140, 140, 183, 592, 724, 727, 730, 914
MroXI GAANNNNTTC 3 cut(s) 317, 378, 833
MseI TTAA 4 cut(s) 89, 422, 588, 594
MspA1I CMGCKG 1 cut(s) 698
MspCI CTTAAG 1 cut(s) 88
NdeI CATATG 1 cut(s) 938
NdeII GATC 2 cut(s) 666, 706
NlaIII CATG 6 cut(s) 104, 274, 308, 326, 371, 977
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 1 cut(s) 977
PaeI GCATGC 1 cut(s) 977
PaeR7I CTCGAG 2 cut(s) 144, 263
PagI TCATGA 1 cut(s) 304
PceI AGGCCT 1 cut(s) 713
PdmI GAANNNNTTC 3 cut(s) 317, 378, 833
PfeI GAWTC 3 cut(s) 185, 289, 313
PkrI GCNGC 1 cut(s) 769
Psp124BI GAGCTC 1 cut(s) 795
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 5 cut(s) 128, 157, 474, 825, 892
RsaNI GTAC 5 cut(s) 127, 156, 473, 824, 891
SacI GAGCTC 1 cut(s) 795
SaqAI TTAA 4 cut(s) 89, 422, 588, 594
SatI GCNGC 1 cut(s) 768
Sau3AI GATC 2 cut(s) 666, 706
ScaI AGTACT 1 cut(s) 825
SduI GDGCHC 1 cut(s) 795
SetI ASST 9 cut(s) 132, 194, 252, 402, 409, 594, 700, 795, 925
SfaNI GCATC 3 cut(s) 163, 406, 556
SfcI CTRYAG 1 cut(s) 627
Sfr274I CTCGAG 2 cut(s) 144, 263
SlaI CTCGAG 2 cut(s) 144, 263
SmlI CTYRAG 5 cut(s) 88, 131, 144, 263, 721
SmoI CTYRAG 5 cut(s) 88, 131, 144, 263, 721
SphI GCATGC 1 cut(s) 977
Sse9I AATT 5 cut(s) 27, 61, 150, 258, 489
SseBI AGGCCT 1 cut(s) 713
SsiI CCGC 1 cut(s) 74
SspMI CTAG 4 cut(s) 482, 522, 582, 683
SstI GAGCTC 1 cut(s) 795
StuI AGGCCT 1 cut(s) 713
TaaI ACNGT 2 cut(s) 608, 735
TaqI TCGA 4 cut(s) 145, 180, 188, 264
TasI AATT 5 cut(s) 27, 61, 150, 258, 489
TatI WGTACW 3 cut(s) 155, 472, 823
TfiI GAWTC 3 cut(s) 185, 289, 313
Tru1I TTAA 4 cut(s) 89, 422, 588, 594
Tru9I TTAA 4 cut(s) 89, 422, 588, 594
TscAI CASTG 1 cut(s) 938
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 5 cut(s) 117, 287, 293, 384, 483
TspGWI ACGGA 2 cut(s) 58, 924
TspRI CASTG 1 cut(s) 938
Vha464I CTTAAG 1 cut(s) 88
XapI RAATTY 3 cut(s) 61, 150, 258
XbaI TCTAGA 2 cut(s) 481, 682
XceI RCATGY 1 cut(s) 977
XcmI CCANNNNNNNNNTGG 1 cut(s) 565
XhoI CTCGAG 2 cut(s) 144, 263
XmnI GAANNNNTTC 3 cut(s) 317, 378, 833
XspI CTAG 4 cut(s) 482, 522, 582, 683
ZrmI AGTACT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.