RchiOBHm_Chr6g0276251

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000508
Physical Location & Seq
Reverse (-)
38247744 .. 38249310
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24782

Sequence Viewer

Length: 1113 bp
ATGGTGTTGAACAAGCGAAGAGTATCTATTCCATTACGGATATCTATTCCTCTTACTTCTCTATGGGCGTGTGATGTCTTCCTAAGTTGCGACCATGAAGACATTTTCTCCGACCACTTGTACCACAAATTGAAGTCTCGAGGAATTTGTACTTTCCAGGATGGCCAACTGTCTCGAGGAATTTGTACTTTCCAGGATGGCCAACAATTGCAAAGAAACACAACTCCTCAGTTTGCCATTGTTGTTCTCTCGCAAAACTATGCTTCTTCGCCCCAGCGCTTGAATGAACTTTCAAAAATTCTAGAGTGCATAAAAGATAGGAACAGGATTCTGCCGGTATTTCGTGATGTTAATCTGTTCCATGTCCAAAAACAAAAAGGGACTTTTGAGAAAGCATTTGAAAAGCATGAAGAAAGGTTTCAGGATGACTTGGAAAAGGTTCAAGCCTGGAGAGATGCTTTAACCCAAGTGTGCAATTTTGCTGGATGGACTACCAATGATAGGAATGAAGTACAGGTTATAGAAGAAATTACTGAAGCACTGTGGAACAAACTACATTCTAGACTCAGTCCAACGGAAAAACTGCACCCGACGTCCACACACAACTTGGTCGCATTCACTAGCATTAGTGTTAATGAGGACAACCATGATCATAACCATCCAATAAACATTACAGATGTGACGGCTACTTCAGTAGAGTGGAACTTTTTGATCACTAGTCTTGGCCTAGAGATCTTGTCAGCGGCTTTTGATCAGGCTTCCTCCTCAAGTGAGCCACACTATGCACTATTTGGTATGCTTTTTTCTATTGCAGCCTTGTTCACTTGCATTTGGGAGCTCATTTACAAGGGCATAAAGAATAGAGTAGTATTGAAGAAGTTCGGAAAGCTCTGGTGGTTTTATTATCCACATCCCCACACTAGCATGCCTTTCGGTACTCTCCCTGAGATTTACGGATTAATTGGAAGCATCGCTCAGTGCATATGCTCTATAACTCAATACATTTACTTCTCTTGGCATGCTGATAGTCCCATAAAACTCTCACTTTTACCAGCAATCTTTCTTTTCTGTTTGGCTGGTTCAAGACTAAATAGGAATCAAAACCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

370

Amino Acids

42.55

Weight (kDa)

6.96

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 190 3.2e-28 TIR domain
TIR_2 PF13676 26 - 117 5.5e-07 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 596
AciI CCGC 1 cut(s) 743
AcoI YGGCCR 2 cut(s) 163, 199
AcsI RAATTY 3 cut(s) 144, 180, 297
AcuI CTGAAG 2 cut(s) 555, 675
AcyI GRCGYC 1 cut(s) 593
AfaI GTAC 5 cut(s) 122, 151, 187, 513, 937
AfeI AGCGCT 1 cut(s) 278
AfiI CCNNNNNNNGG 1 cut(s) 501
AgsI TTSAA 8 cut(s) 10, 133, 283, 294, 401, 443, 874, 1083
AhlI ACTAGT 1 cut(s) 716
AjnI CCWGG 3 cut(s) 156, 192, 446
AluBI AGCT 2 cut(s) 838, 889
AluI AGCT 2 cut(s) 838, 889
Alw21I GWGCWC 1 cut(s) 840
Alw26I GTCTC 2 cut(s) 141, 177
Ama87I CYCGRG 2 cut(s) 138, 174
Aor51HI AGCGCT 1 cut(s) 278
AoxI GGCC 3 cut(s) 163, 199, 724
ApeKI GCWGC 1 cut(s) 812
ApoI RAATTY 3 cut(s) 144, 180, 297
AseI ATTAAT 1 cut(s) 959
Asp700I GAANNNNTTC 3 cut(s) 417, 438, 878
AspLEI GCGC 1 cut(s) 279
AvaI CYCGRG 2 cut(s) 138, 174
BalI TGGCCA 2 cut(s) 165, 201
BanII GRGCYC 1 cut(s) 840
BbsI GAAGAC 2 cut(s) 70, 105
Bbv12I GWGCWC 1 cut(s) 840
BbvI GCAGC 1 cut(s) 824
BccI CCATC 4 cut(s) 155, 191, 480, 666
BceAI ACGGC 1 cut(s) 699
BcgI CGANNNNNNTGC 2 cut(s) 913, 947
BciT130I CCWGG 3 cut(s) 158, 194, 448
BclI TGATCA 3 cut(s) 649, 711, 751
BcoDI GTCTC 2 cut(s) 141, 177
BcuI ACTAGT 1 cut(s) 716
BfaI CTAG 6 cut(s) 302, 561, 621, 717, 728, 921
BfoI RGCGCY 1 cut(s) 280
BglII AGATCT 1 cut(s) 732
BisI GCNGC 2 cut(s) 744, 813
BlsI GCNGC 2 cut(s) 745, 814
Bme1390I CCNGG 3 cut(s) 158, 194, 448
BmeT110I CYCGRG 2 cut(s) 138, 174
BmrFI CCNGG 3 cut(s) 158, 194, 448
BmsI GCATC 2 cut(s) 445, 978
BpiI GAAGAC 2 cut(s) 70, 105
BpmI CTGGAG 1 cut(s) 469
BpuEI CTTGAG 1 cut(s) 751
BsaBI GATNNNNATC 1 cut(s) 351
BsaHI GRCGYC 1 cut(s) 593
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 1 cut(s) 501
Bse118I RCCGGY 1 cut(s) 334
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 3 cut(s) 158, 194, 448
BseGI GGATG 6 cut(s) 166, 202, 430, 491, 658, 910
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 501
BseMII CTCAG 4 cut(s) 242, 580, 936, 989
BseRI GAGGAG 2 cut(s) 216, 754
BseXI GCAGC 1 cut(s) 824
BseYI CCCAGC 1 cut(s) 273
BsgI GTGCAG 1 cut(s) 569
BshFI GGCC 3 cut(s) 165, 201, 726
BsiHKAI GWGCWC 1 cut(s) 840
BsiHKCI CYCGRG 2 cut(s) 138, 174
BsiSI CCGG 1 cut(s) 335
BslFI GGGAC 2 cut(s) 394, 1014
BslI CCNNNNNNNGG 1 cut(s) 501
BsmAI GTCTC 2 cut(s) 141, 177
BsmFI GGGAC 2 cut(s) 394, 1014
BsmI GAATGC 1 cut(s) 614
BsnI GGCC 3 cut(s) 165, 201, 726
BsoBI CYCGRG 2 cut(s) 138, 174
Bsp1286I GDGCHC 1 cut(s) 840
Bsp143I GATC 4 cut(s) 649, 711, 732, 751
BspACI CCGC 1 cut(s) 743
BspANI GGCC 3 cut(s) 165, 201, 726
BspCNI CTCAG 4 cut(s) 241, 579, 937, 988
BsrFI RCCGGY 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 334
BssMI GATC 4 cut(s) 649, 711, 732, 751
BssNI GRCGYC 1 cut(s) 593
Bst2UI CCWGG 3 cut(s) 158, 194, 448
Bst4CI ACNGT 2 cut(s) 171, 543
Bst6I CTCTTC 1 cut(s) 13
BstACI GRCGYC 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 926, 1020
BstDEI CTNAG 5 cut(s) 83, 228, 566, 945, 975
BstF5I GGATG 6 cut(s) 166, 202, 430, 491, 658, 910
BstH2I RGCGCY 1 cut(s) 280
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 4 cut(s) 652, 714, 735, 754
BstMAI GTCTC 2 cut(s) 141, 177
BstMBI GATC 4 cut(s) 649, 711, 732, 751
BstNI CCWGG 3 cut(s) 158, 194, 448
BstNSI RCATGY 2 cut(s) 928, 1022
BstSCI CCNGG 3 cut(s) 156, 192, 446
BstV1I GCAGC 1 cut(s) 824
BstV2I GAAGAC 2 cut(s) 70, 105
BstX2I RGATCY 1 cut(s) 732
BstYI RGATCY 1 cut(s) 732
BsuRI GGCC 3 cut(s) 165, 201, 726
BtgZI GCGATG 1 cut(s) 955
BtsCI GGATG 6 cut(s) 166, 202, 430, 491, 658, 910
BtsIMutI CAGTG 2 cut(s) 539, 983
Cac8I GCNNGC 2 cut(s) 926, 1020
CfoI GCGC 1 cut(s) 279
Cfr10I RCCGGY 1 cut(s) 334
Csp6I GTAC 5 cut(s) 121, 150, 186, 512, 936
CviAII CATG 6 cut(s) 95, 362, 407, 647, 925, 1019
CviQI GTAC 5 cut(s) 121, 150, 186, 512, 936
DdeI CTNAG 5 cut(s) 83, 228, 566, 945, 975
DpnI GATC 4 cut(s) 651, 713, 734, 753
DpnII GATC 4 cut(s) 649, 711, 732, 751
EaeI YGGCCR 2 cut(s) 163, 199
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 838
Eco24I GRGCYC 1 cut(s) 840
Eco32I GATATC 1 cut(s) 42
Eco47III AGCGCT 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 838
Eco57I CTGAAG 2 cut(s) 555, 675
Eco88I CYCGRG 2 cut(s) 138, 174
EcoICRI GAGCTC 1 cut(s) 838
EcoRII CCWGG 3 cut(s) 156, 192, 446
EcoRV GATATC 1 cut(s) 42
EcoT38I GRGCYC 1 cut(s) 840
FaeI CATG 6 cut(s) 98, 365, 410, 650, 928, 1022
FaqI GGGAC 2 cut(s) 394, 1014
FatI CATG 6 cut(s) 94, 361, 406, 646, 924, 1018
FauNDI CATATG 1 cut(s) 983
FbaI TGATCA 3 cut(s) 649, 711, 751
Fnu4HI GCNGC 2 cut(s) 744, 813
FokI GGATG 6 cut(s) 173, 209, 437, 498, 645, 897
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 2 cut(s) 744, 813
FspBI CTAG 6 cut(s) 302, 561, 621, 717, 728, 921
GlaI GCGC 1 cut(s) 278
GluI GCNGC 2 cut(s) 744, 813
GsaI CCCAGC 1 cut(s) 277
GsuI CTGGAG 1 cut(s) 469
HaeII RGCGCY 1 cut(s) 280
HaeIII GGCC 3 cut(s) 165, 201, 726
HapII CCGG 1 cut(s) 335
HhaI GCGC 1 cut(s) 279
Hin1I GRCGYC 1 cut(s) 593
Hin1II CATG 6 cut(s) 98, 365, 410, 650, 928, 1022
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HinfI GANTC 3 cut(s) 328, 564, 1096
HpaII CCGG 1 cut(s) 335
Hpy166II GTNNAC 2 cut(s) 597, 822
Hpy188I TCNGA 2 cut(s) 112, 884
Hpy188III TCNNGA 7 cut(s) 138, 174, 302, 344, 422, 561, 1083
Hpy8I GTNNAC 2 cut(s) 597, 822
Hpy99I CGWCG 1 cut(s) 595
HpyCH4III ACNGT 2 cut(s) 171, 543
HpyCH4IV ACGT 1 cut(s) 593
HpyCH4V TGCA 8 cut(s) 211, 309, 474, 586, 785, 812, 828, 981
HpyF3I CTNAG 5 cut(s) 83, 228, 566, 945, 975
HpySE526I ACGT 1 cut(s) 593
Hsp92I GRCGYC 1 cut(s) 593
Hsp92II CATG 6 cut(s) 98, 365, 410, 650, 928, 1022
HspAI GCGC 1 cut(s) 277
Ksp22I TGATCA 3 cut(s) 649, 711, 751
Kzo9I GATC 4 cut(s) 649, 711, 732, 751
LmnI GCTCC 1 cut(s) 835
Lsp1109I GCAGC 1 cut(s) 824
LweI GCATC 2 cut(s) 445, 978
MaeI CTAG 6 cut(s) 302, 561, 621, 717, 728, 921
MaeII ACGT 1 cut(s) 593
MaeIII GTNAC 1 cut(s) 679
MalI GATC 4 cut(s) 651, 713, 734, 753
MboI GATC 4 cut(s) 649, 711, 732, 751
MboII GAAGA 7 cut(s) 30, 70, 110, 258, 422, 536, 886
MfeI CAATTG 1 cut(s) 206
MflI RGATCY 1 cut(s) 732
MhlI GDGCHC 1 cut(s) 840
MlsI TGGCCA 2 cut(s) 165, 201
MluCI AATT 8 cut(s) 128, 144, 180, 206, 297, 475, 528, 960
MluNI TGGCCA 2 cut(s) 165, 201
MlyI GAGTC 1 cut(s) 558
MmeI TCCRAC 2 cut(s) 135, 596
MnlI CCTC 7 cut(s) 60, 134, 170, 237, 631, 772, 775
Mox20I TGGCCA 2 cut(s) 165, 201
MroXI GAANNNNTTC 3 cut(s) 417, 438, 878
MscI TGGCCA 2 cut(s) 165, 201
MseI TTAA 4 cut(s) 351, 461, 633, 959
MslI CAYNNNNRTG 1 cut(s) 923
Msp20I TGGCCA 2 cut(s) 165, 201
MspA1I CMGCKG 1 cut(s) 743
MspI CCGG 1 cut(s) 335
MspR9I CCNGG 3 cut(s) 158, 194, 448
MunI CAATTG 1 cut(s) 206
Mva1269I GAATGC 1 cut(s) 614
MvaI CCWGG 3 cut(s) 158, 194, 448
NdeI CATATG 1 cut(s) 983
NdeII GATC 4 cut(s) 649, 711, 732, 751
NlaIII CATG 6 cut(s) 98, 365, 410, 650, 928, 1022
NmuCI GTSAC 1 cut(s) 679
NspI RCATGY 2 cut(s) 928, 1022
PaeI GCATGC 2 cut(s) 928, 1022
PaeR7I CTCGAG 2 cut(s) 138, 174
PctI GAATGC 1 cut(s) 614
PdmI GAANNNNTTC 3 cut(s) 417, 438, 878
PfeI GAWTC 2 cut(s) 328, 1096
PflFI GACNNNGTC 1 cut(s) 567
PfoI TCCNGGA 2 cut(s) 156, 192
PkrI GCNGC 2 cut(s) 745, 814
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
PshBI ATTAAT 1 cut(s) 959
Psp124BI GAGCTC 1 cut(s) 840
Psp6I CCWGG 3 cut(s) 156, 192, 446
PspFI CCCAGC 1 cut(s) 273
PspGI CCWGG 3 cut(s) 156, 192, 446
PsuI RGATCY 1 cut(s) 732
PsyI GACNNNGTC 1 cut(s) 567
RsaI GTAC 5 cut(s) 122, 151, 187, 513, 937
RsaNI GTAC 5 cut(s) 121, 150, 186, 512, 936
RseI CAYNNNNRTG 1 cut(s) 923
SacI GAGCTC 1 cut(s) 840
SaqAI TTAA 4 cut(s) 351, 461, 633, 959
SatI GCNGC 2 cut(s) 744, 813
Sau3AI GATC 4 cut(s) 649, 711, 732, 751
SchI GAGTC 1 cut(s) 558
ScrFI CCNGG 3 cut(s) 158, 194, 448
SduI GDGCHC 1 cut(s) 840
SetI ASST 6 cut(s) 419, 441, 519, 596, 840, 891
SfaNI GCATC 2 cut(s) 445, 978
Sfr274I CTCGAG 2 cut(s) 138, 174
SlaI CTCGAG 2 cut(s) 138, 174
SmiMI CAYNNNNRTG 1 cut(s) 923
SmlI CTYRAG 3 cut(s) 138, 174, 766
SmoI CTYRAG 3 cut(s) 138, 174, 766
SpeI ACTAGT 1 cut(s) 716
SphI GCATGC 2 cut(s) 928, 1022
Sse9I AATT 8 cut(s) 128, 144, 180, 206, 297, 475, 528, 960
SsiI CCGC 1 cut(s) 743
SspMI CTAG 6 cut(s) 302, 561, 621, 717, 728, 921
SstI GAGCTC 1 cut(s) 840
StyD4I CCNGG 3 cut(s) 156, 192, 446
TaaI ACNGT 2 cut(s) 171, 543
TaiI ACGT 1 cut(s) 596
TaqI TCGA 2 cut(s) 139, 175
TasI AATT 8 cut(s) 128, 144, 180, 206, 297, 475, 528, 960
TatI WGTACW 3 cut(s) 149, 185, 511
TauI GCSGC 1 cut(s) 746
TfiI GAWTC 2 cut(s) 328, 1096
Tru1I TTAA 4 cut(s) 351, 461, 633, 959
Tru9I TTAA 4 cut(s) 351, 461, 633, 959
TscAI CASTG 2 cut(s) 546, 983
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 1 cut(s) 812
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 4 cut(s) 111, 300, 423, 522
TspGWI ACGGA 3 cut(s) 52, 590, 969
TspRI CASTG 2 cut(s) 546, 983
Tth111I GACNNNGTC 1 cut(s) 567
VspI ATTAAT 1 cut(s) 959
XapI RAATTY 3 cut(s) 144, 180, 297
XbaI TCTAGA 2 cut(s) 301, 560
XceI RCATGY 2 cut(s) 928, 1022
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XhoI CTCGAG 2 cut(s) 138, 174
XmnI GAANNNNTTC 3 cut(s) 417, 438, 878
XspI CTAG 6 cut(s) 302, 561, 621, 717, 728, 921
ZraI GACGTC 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.