Rw6G018380

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000508
Physical Location & Seq
Reverse (-)
36545317 .. 36547128
1812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G018380.1

Sequence Viewer

Length: 1101 bp
ATGGTGTTGAAGAAGCGAAGAGCATCTATTCCATTACGGATATCTATTCCTTTTACTTCTCGGGCGTGTGATGTCTTCCTAAGTTGCGAGTATGAAGACACCTTCTCCTCCCACTTGTACCACAAATTGCAGTCTCGAGGAATTAGTACTTTCCAGGATGGCCAACAATTGCAAAGAAACGCAACTCCTCTTGAGCTCTTTGGTGCGATCGAAGAATCGAAATTTGCCATTGTTGTTCTCTCGCAAAACTATGCTTCTTCGCCCCAGCGCTTGAATGAACTTTCCAAAATTCTGGAGTGCATGAAAGATAGGAACAGGATTCTGCCGGTGTTTCGTGATGTTAATCTGTTCCATGTCCAAAAACAAAAAGGGGCTTTTGAGAAAGCATTTGAAAAGCATGAAGAAAGGTTTCAGGATGACTTGGAAAAGGTTCAAGCCTGGAGAGATGCTTTAACCCAAGTGTGCAATTTTGCTGGATGGACTACCAATGATAGGAATGAAGTACAGGTTATAGAAGAAATTACTGAAGCACTGTGGAACAAACTACATTCTAGACTCAGTCCAACCGAAAAGCTGCACCCAACATCCACACACAACTTGGTCGCATTCACTAGCATTAGTGTTAATGAGGACCACCGTGACCATAACCATCCAATAAACATTACAGATGTGACGGCTACTTCAGTAGAGTGGAAGTTTTTGATTACTAACCTTGGCCTAGAGATTTTGTCAGCTGCTTTTGATCAGGCTTCCTCGTCAAGTAAGCCACACTATGCACTATTTGGTATGCTGTTTTCTATTGCAGCCTTGTTCACTTGCATTTGGGAGCTCATTTACAAGGGCATAAAGAATAGAGTAGTATTGAAGAAGTTCGGAATGCTCTGGTGGTTTTATTATCCACATCCCCACAATATCATGCCTTTCGGTACTCTCCCTGAGATTTACGGATTAATTGGAAGCATCGCTCAGTGCATATGCTCTATAACTCAATACATTTACTTCTCTTGGCATGCTGATAGTCCCATAAAACTCTCACTTTTACCAGCAATCTTTCTTTTCTGTTTGGCTGGTTCAAGACTAAATAGGAATCAAAACCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

366

Amino Acids

42.17

Weight (kDa)

8.07

Isoelectric Point (pI)

54.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 187 1.7e-35 TIR domain
TIR_2 PF13676 25 - 114 3e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 2 cut(s) 221, 288
AcuI CTGAAG 2 cut(s) 546, 666
AfaI GTAC 4 cut(s) 119, 148, 504, 928
AfeI AGCGCT 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 492
AgsI TTSAA 6 cut(s) 10, 274, 392, 434, 865, 1074
AjnI CCWGG 2 cut(s) 153, 437
AluBI AGCT 4 cut(s) 196, 574, 734, 829
AluI AGCT 4 cut(s) 196, 574, 734, 829
Alw21I GWGCWC 2 cut(s) 198, 831
Alw26I GTCTC 1 cut(s) 138
Ama87I CYCGRG 2 cut(s) 60, 135
Aor51HI AGCGCT 1 cut(s) 269
AoxI GGCC 2 cut(s) 160, 715
ApeKI GCWGC 3 cut(s) 574, 734, 803
ApoI RAATTY 2 cut(s) 221, 288
AseI ATTAAT 1 cut(s) 950
Asp700I GAANNNNTTC 3 cut(s) 408, 429, 869
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 631
AvaI CYCGRG 2 cut(s) 60, 135
AvaII GGWCC 1 cut(s) 631
BalI TGGCCA 1 cut(s) 162
BanII GRGCYC 2 cut(s) 198, 831
BbsI GAAGAC 2 cut(s) 67, 102
Bbv12I GWGCWC 2 cut(s) 198, 831
BbvI GCAGC 3 cut(s) 561, 721, 815
BccI CCATC 3 cut(s) 152, 471, 657
BceAI ACGGC 1 cut(s) 690
BciT130I CCWGG 2 cut(s) 155, 439
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 3 cut(s) 552, 612, 719
BfoI RGCGCY 1 cut(s) 271
BisI GCNGC 3 cut(s) 575, 735, 804
BlsI GCNGC 3 cut(s) 576, 736, 805
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 2 cut(s) 155, 439
Bme18I GGWCC 1 cut(s) 631
BmeT110I CYCGRG 2 cut(s) 60, 135
BmgT120I GGNCC 1 cut(s) 631
BmrFI CCNGG 2 cut(s) 155, 439
BmsI GCATC 3 cut(s) 32, 436, 969
BpiI GAAGAC 2 cut(s) 67, 102
BpmI CTGGAG 2 cut(s) 314, 460
BpuEI CTTGAG 1 cut(s) 212
BsaBI GATNNNNATC 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 712
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse118I RCCGGY 1 cut(s) 325
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 2 cut(s) 155, 439
BseDI CCNNGG 1 cut(s) 712
BseGI GGATG 6 cut(s) 163, 421, 482, 584, 649, 901
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 492
BseMII CTCAG 3 cut(s) 571, 927, 980
BseRI GAGGAG 2 cut(s) 97, 177
BseXI GCAGC 3 cut(s) 561, 721, 815
BseYI CCCAGC 1 cut(s) 264
BsgI GTGCAG 1 cut(s) 560
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 162, 717
BsiEI CGRYCG 1 cut(s) 210
BsiHKAI GWGCWC 2 cut(s) 198, 831
BsiHKCI CYCGRG 2 cut(s) 60, 135
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 1 cut(s) 1005
BslI CCNNNNNNNGG 1 cut(s) 492
BsmAI GTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 1005
BsmI GAATGC 2 cut(s) 605, 882
BsnI GGCC 2 cut(s) 162, 717
BsoBI CYCGRG 2 cut(s) 60, 135
Bsp1286I GDGCHC 2 cut(s) 198, 831
Bsp143I GATC 2 cut(s) 207, 742
BspANI GGCC 2 cut(s) 162, 717
BspCNI CTCAG 3 cut(s) 570, 928, 979
BspQI GCTCTTC 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 712
BssMI GATC 2 cut(s) 207, 742
BssT1I CCWWGG 1 cut(s) 712
Bst2UI CCWGG 2 cut(s) 155, 439
Bst4CI ACNGT 2 cut(s) 534, 638
Bst6I CTCTTC 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 1011
BstDEI CTNAG 4 cut(s) 80, 557, 936, 966
BstF5I GGATG 6 cut(s) 163, 421, 482, 584, 649, 901
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 2 cut(s) 210, 745
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 2 cut(s) 207, 742
BstMCI CGRYCG 1 cut(s) 210
BstNI CCWGG 2 cut(s) 155, 439
BstNSI RCATGY 1 cut(s) 1013
BstSCI CCNGG 2 cut(s) 153, 437
BstV1I GCAGC 3 cut(s) 561, 721, 815
BstV2I GAAGAC 2 cut(s) 67, 102
BstXI CCANNNNNNTGG 1 cut(s) 292
BsuRI GGCC 2 cut(s) 162, 717
BtgZI GCGATG 1 cut(s) 946
BtsCI GGATG 6 cut(s) 163, 421, 482, 584, 649, 901
BtsIMutI CAGTG 2 cut(s) 530, 974
Cac8I GCNNGC 1 cut(s) 1011
CfoI GCGC 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 1 cut(s) 631
Csp6I GTAC 4 cut(s) 118, 147, 503, 927
CviAII CATG 5 cut(s) 301, 353, 398, 916, 1010
CviQI GTAC 4 cut(s) 118, 147, 503, 927
DdeI CTNAG 4 cut(s) 80, 557, 936, 966
DpnI GATC 2 cut(s) 209, 744
DpnII GATC 2 cut(s) 207, 742
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Ecl136II GAGCTC 2 cut(s) 196, 829
Eco130I CCWWGG 1 cut(s) 712
Eco24I GRGCYC 2 cut(s) 198, 831
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 631
Eco47III AGCGCT 1 cut(s) 269
Eco53kI GAGCTC 2 cut(s) 196, 829
Eco57I CTGAAG 2 cut(s) 546, 666
Eco88I CYCGRG 2 cut(s) 60, 135
EcoICRI GAGCTC 2 cut(s) 196, 829
EcoRII CCWGG 2 cut(s) 153, 437
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 712
EcoT38I GRGCYC 2 cut(s) 198, 831
ErhI CCWWGG 1 cut(s) 712
FaeI CATG 5 cut(s) 304, 356, 401, 919, 1013
FaqI GGGAC 1 cut(s) 1005
FatI CATG 5 cut(s) 300, 352, 397, 915, 1009
FauNDI CATATG 1 cut(s) 974
FbaI TGATCA 1 cut(s) 742
Fnu4HI GCNGC 3 cut(s) 575, 735, 804
FokI GGATG 6 cut(s) 170, 428, 489, 571, 636, 888
FriOI GRGCYC 2 cut(s) 198, 831
Fsp4HI GCNGC 3 cut(s) 575, 735, 804
FspBI CTAG 3 cut(s) 552, 612, 719
GlaI GCGC 1 cut(s) 269
GluI GCNGC 3 cut(s) 575, 735, 804
GsaI CCCAGC 1 cut(s) 268
GsuI CTGGAG 2 cut(s) 314, 460
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 2 cut(s) 162, 717
HapII CCGG 1 cut(s) 326
HhaI GCGC 1 cut(s) 270
Hin1II CATG 5 cut(s) 304, 356, 401, 919, 1013
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HinfI GANTC 4 cut(s) 215, 319, 555, 1087
HpaII CCGG 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 813
Hpy188I TCNGA 1 cut(s) 875
Hpy188III TCNNGA 7 cut(s) 135, 191, 293, 335, 413, 552, 1074
Hpy8I GTNNAC 1 cut(s) 813
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 2 cut(s) 534, 638
HpyCH4V TGCA 9 cut(s) 130, 172, 300, 465, 577, 776, 803, 819, 972
HpyF3I CTNAG 4 cut(s) 80, 557, 936, 966
Hsp92II CATG 5 cut(s) 304, 356, 401, 919, 1013
HspAI GCGC 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 2 cut(s) 207, 742
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 1 cut(s) 826
Lsp1109I GCAGC 3 cut(s) 561, 721, 815
LweI GCATC 3 cut(s) 32, 436, 969
MaeI CTAG 3 cut(s) 552, 612, 719
MaeIII GTNAC 2 cut(s) 638, 670
MalI GATC 2 cut(s) 209, 744
MboI GATC 2 cut(s) 207, 742
MboII GAAGA 9 cut(s) 22, 30, 67, 107, 224, 249, 413, 527, 877
MfeI CAATTG 1 cut(s) 167
MhlI GDGCHC 2 cut(s) 198, 831
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 8 cut(s) 125, 141, 167, 221, 288, 466, 519, 951
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 1 cut(s) 549
MmeI TCCRAC 1 cut(s) 587
MnlI CCTC 5 cut(s) 118, 131, 198, 622, 763
Mox20I TGGCCA 1 cut(s) 162
MroXI GAANNNNTTC 3 cut(s) 408, 429, 869
MscI TGGCCA 1 cut(s) 162
MseI TTAA 4 cut(s) 342, 452, 624, 950
Msp20I TGGCCA 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 2 cut(s) 155, 439
MunI CAATTG 1 cut(s) 167
Mva1269I GAATGC 2 cut(s) 605, 882
MvaI CCWGG 2 cut(s) 155, 439
NdeI CATATG 1 cut(s) 974
NdeII GATC 2 cut(s) 207, 742
NlaIII CATG 5 cut(s) 304, 356, 401, 919, 1013
NmuCI GTSAC 2 cut(s) 638, 670
NspI RCATGY 1 cut(s) 1013
PaeI GCATGC 1 cut(s) 1013
PaeR7I CTCGAG 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 13
PctI GAATGC 2 cut(s) 605, 882
PdmI GAANNNNTTC 3 cut(s) 408, 429, 869
PfeI GAWTC 3 cut(s) 215, 319, 1087
PflFI GACNNNGTC 1 cut(s) 558
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 3 cut(s) 576, 736, 805
Ple19I CGATCG 1 cut(s) 210
PleI GAGTC 1 cut(s) 549
PpsI GAGTC 1 cut(s) 549
PshBI ATTAAT 1 cut(s) 950
Psp124BI GAGCTC 2 cut(s) 198, 831
Psp6I CCWGG 2 cut(s) 153, 437
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 2 cut(s) 153, 437
PspPI GGNCC 1 cut(s) 631
PsyI GACNNNGTC 1 cut(s) 558
PvuI CGATCG 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 4 cut(s) 119, 148, 504, 928
RsaNI GTAC 4 cut(s) 118, 147, 503, 927
SacI GAGCTC 2 cut(s) 198, 831
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 4 cut(s) 342, 452, 624, 950
SatI GCNGC 3 cut(s) 575, 735, 804
Sau3AI GATC 2 cut(s) 207, 742
Sau96I GGNCC 1 cut(s) 631
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 1 cut(s) 549
ScrFI CCNGG 2 cut(s) 155, 439
SduI GDGCHC 2 cut(s) 198, 831
SetI ASST 9 cut(s) 104, 198, 410, 432, 510, 576, 714, 736, 831
SfaNI GCATC 3 cut(s) 32, 436, 969
Sfr274I CTCGAG 1 cut(s) 135
SinI GGWCC 1 cut(s) 631
SlaI CTCGAG 1 cut(s) 135
SmlI CTYRAG 2 cut(s) 135, 191
SmoI CTYRAG 2 cut(s) 135, 191
SphI GCATGC 1 cut(s) 1013
Sse9I AATT 8 cut(s) 125, 141, 167, 221, 288, 466, 519, 951
SspMI CTAG 3 cut(s) 552, 612, 719
SstI GAGCTC 2 cut(s) 198, 831
StyD4I CCNGG 2 cut(s) 153, 437
StyI CCWWGG 1 cut(s) 712
TaaI ACNGT 2 cut(s) 534, 638
TaqI TCGA 3 cut(s) 136, 210, 218
TasI AATT 8 cut(s) 125, 141, 167, 221, 288, 466, 519, 951
TatI WGTACW 2 cut(s) 146, 502
TfiI GAWTC 3 cut(s) 215, 319, 1087
Tru1I TTAA 4 cut(s) 342, 452, 624, 950
Tru9I TTAA 4 cut(s) 342, 452, 624, 950
TscAI CASTG 2 cut(s) 537, 974
TseFI GTSAC 2 cut(s) 638, 670
TseI GCWGC 3 cut(s) 574, 734, 803
Tsp45I GTSAC 2 cut(s) 638, 670
TspDTI ATGAA 5 cut(s) 108, 291, 317, 414, 513
TspGWI ACGGA 2 cut(s) 52, 960
TspRI CASTG 2 cut(s) 537, 974
Tth111I GACNNNGTC 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 631
VspI ATTAAT 1 cut(s) 950
XapI RAATTY 2 cut(s) 221, 288
XbaI TCTAGA 1 cut(s) 551
XceI RCATGY 1 cut(s) 1013
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XhoI CTCGAG 1 cut(s) 135
XmnI GAANNNNTTC 3 cut(s) 408, 429, 869
XspI CTAG 3 cut(s) 552, 612, 719
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.