RchiOBHm_Chr6g0276271

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000508
Physical Location & Seq
Reverse (-)
38261285 .. 38264138
2854 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24784

Sequence Viewer

Length: 1143 bp
ATGGTGTTGAACAAGCAAAGATCCTTACCTAGTCCATTAGCCGTATCTATTCCTGCAACTAATCTATGGACTTTCGATGTCTTCTTGAGTTGCGACCATGAAAACATCTTCTCATCCCACTTATACCACAAGTTGCAGTCTCGACGTATTTTTACTTTCTTTGGAAGAAACACAACTACCCCTCTGGAGCTCTTCGGTGCGATCGAAGAATCGAGGTTTGCAATTGTTGTTCTCTCGCAAAACTATGCTTCTTCACCCCAGCGCCTGAATGAACTTACAAAAATTCTCCAGTGCATGAAAGATAGGAACCGGATTCTGCCCATCTTTCATGATGTGAATCTCTCCCATGTGCAGAAGCAAAAGGATAGTTTTGGGAAAGCCTTTGACGAGCACTTAAAAAGGTTTCACGATGACTTGGCAAAGGTTGAAGCTTGGAGAGATGCTTTAACCAAAGTGTGCAATTTTGCTGGATGGACTTCCAATGACAGGAATGAAGTAGAGGTTATCGAAGAAATTACGGAAGCACTGTGGAACAAACTACATTTTAGACAAAGTCCAAAGGAAAAACTACATCCTACATACACATTCTTAGAGTCCACACACTTGGTCGCATTAGCTAGCATTAGTTCCCCTCAGCAGAACCGTGACCATGACAAGCCAAAGAAAATTACGGATGCAATGGCTACTCCAGTGGAGTGGATCTTTCTACTCACTAACCTTGGCCTAGAGATTTTGTCAGCTGCTTTTGATCAGGCTTCCTCCCCAAGTAAGCCACACTATGCACTATTTGGTGTGCTGTTTGCTATTGCAGCTATGTTTGTTTGCATTTGGGAGCTCATTCACAAGGGCATCAATGAAAAAGTAGTATTGAAGAAGTTTGGAAAGCTATGGTGGTATTATTATCCAGACCCCCACGAAAGCACGCCTTTTGGTACCCTCCCTGACATTTATGGACTAATTGCAGGAATTTCTCAGTGTATATGCTCCATAACTCAATATGTTTACTTCTCTTGGCATGCTGATAGTCCCATAAAACTATCCCTTTTACCTGCCATCTTTCTTTTGTGTTTGGCCGGTTCAAGACTAAATAGAAATCGAAACCATGGTCAGACTGTTGGACCTAAATCCCTAGACAAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

380

Amino Acids

43.49

Weight (kDa)

8.36

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 185 6.1e-33 TIR domain
TIR_2 PF13676 27 - 114 1e-07 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1057
Acc65I GGTACC 1 cut(s) 932
AccB1I GGYRCC 1 cut(s) 932
AclWI GGATC 2 cut(s) 15, 707
AcoI YGGCCR 1 cut(s) 1071
AcsI RAATTY 2 cut(s) 282, 966
AfaI GTAC 1 cut(s) 934
AfiI CCNNNNNNNGG 1 cut(s) 486
AgsI TTSAA 4 cut(s) 10, 428, 871, 1080
AluBI AGCT 7 cut(s) 190, 431, 617, 740, 812, 835, 886
AluI AGCT 7 cut(s) 190, 431, 617, 740, 812, 835, 886
Alw21I GWGCWC 3 cut(s) 192, 393, 837
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 2 cut(s) 15, 707
AlwNI CAGNNNCTG 1 cut(s) 265
AoxI GGCC 2 cut(s) 721, 1071
ApeKI GCWGC 2 cut(s) 740, 809
ApoI RAATTY 2 cut(s) 282, 966
ArsI GACNNNNNNTTYG 2 cut(s) 1090, 1122
Asp718I GGTACC 1 cut(s) 932
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 1118
AsuHPI GGTGA 1 cut(s) 246
AsuNHI GCTAGC 1 cut(s) 617
AvaII GGWCC 1 cut(s) 1118
BanI GGYRCC 1 cut(s) 932
BanII GRGCYC 2 cut(s) 192, 837
BbsI GAAGAC 1 cut(s) 73
Bbv12I GWGCWC 3 cut(s) 192, 393, 837
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 2 cut(s) 727, 821
BccI CCATC 3 cut(s) 329, 465, 1061
BceAI ACGGC 1 cut(s) 26
BclI TGATCA 1 cut(s) 748
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 4 cut(s) 30, 618, 725, 1130
BfoI RGCGCY 1 cut(s) 265
BfuAI ACCTGC 1 cut(s) 1057
BisI GCNGC 2 cut(s) 741, 810
BlsI GCNGC 2 cut(s) 742, 811
Bme18I GGWCC 1 cut(s) 1118
BmgT120I GGNCC 1 cut(s) 1118
BmiI GGNNCC 2 cut(s) 308, 934
BmsI GCATC 3 cut(s) 430, 664, 858
BmtI GCTAGC 1 cut(s) 621
BpiI GAAGAC 1 cut(s) 73
BpmI CTGGAG 3 cut(s) 206, 272, 672
Bpu10I CCTNAGC 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 106
BsaBI GATNNNNATC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 718, 1102
BsaWI WCCGGW 1 cut(s) 309
Bsc4I CCNNNNNNNGG 1 cut(s) 486
Bse118I RCCGGY 1 cut(s) 1073
Bse1I ACTGG 2 cut(s) 289, 689
Bse3DI GCAATG 1 cut(s) 684
Bse8I GATNNNNATC 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 718, 1102
BseGI GGATG 4 cut(s) 113, 476, 571, 679
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 1 cut(s) 486
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 2 cut(s) 647, 986
BseNI ACTGG 2 cut(s) 289, 689
BseXI GCAGC 2 cut(s) 727, 821
BseYI CCCAGC 1 cut(s) 258
BsgI GTGCAG 1 cut(s) 371
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 723, 1073
BshNI GGYRCC 1 cut(s) 932
BsiEI CGRYCG 1 cut(s) 204
BsiHKAI GWGCWC 3 cut(s) 192, 393, 837
BsiSI CCGG 2 cut(s) 310, 1074
BslFI GGGAC 1 cut(s) 1011
BslI CCNNNNNNNGG 1 cut(s) 486
BsmAI GTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 1011
BsnI GGCC 2 cut(s) 723, 1073
Bsp1286I GDGCHC 3 cut(s) 192, 393, 837
Bsp143I GATC 4 cut(s) 20, 201, 699, 748
Bsp19I CCATGG 1 cut(s) 1102
BspANI GGCC 2 cut(s) 723, 1073
BspCNI CTCAG 2 cut(s) 646, 985
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 308, 934
BspMI ACCTGC 1 cut(s) 1057
BspOI GCTAGC 1 cut(s) 621
BspPI GGATC 2 cut(s) 15, 707
BspQI GCTCTTC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 932
BsrDI GCAATG 1 cut(s) 684
BsrFI RCCGGY 1 cut(s) 1073
BsrI ACTGG 2 cut(s) 289, 689
BssAI RCCGGY 1 cut(s) 1073
BssECI CCNNGG 2 cut(s) 718, 1102
BssMI GATC 4 cut(s) 20, 201, 699, 748
BssT1I CCWWGG 2 cut(s) 718, 1102
Bst4CI ACNGT 3 cut(s) 528, 644, 1114
Bst6I CTCTTC 1 cut(s) 197
BstC8I GCNNGC 3 cut(s) 619, 923, 1017
BstDEI CTNAG 3 cut(s) 589, 633, 972
BstDSI CCRYGG 1 cut(s) 1102
BstF5I GGATG 4 cut(s) 113, 476, 571, 679
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 4 cut(s) 23, 204, 702, 751
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 4 cut(s) 20, 201, 699, 748
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 1 cut(s) 809
BstNSI RCATGY 1 cut(s) 1019
BstV1I GCAGC 2 cut(s) 727, 821
BstV2I GAAGAC 1 cut(s) 73
BstX2I RGATCY 2 cut(s) 20, 699
BstXI CCANNNNNNTGG 2 cut(s) 604, 696
BstYI RGATCY 2 cut(s) 20, 699
BsuRI GGCC 2 cut(s) 723, 1073
BtgI CCRYGG 1 cut(s) 1102
BtsCI GGATG 4 cut(s) 113, 476, 571, 679
BtsIMutI CAGTG 4 cut(s) 296, 524, 696, 980
BveI ACCTGC 1 cut(s) 1057
Cac8I GCNNGC 3 cut(s) 619, 923, 1017
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 328
CfoI GCGC 1 cut(s) 264
Cfr10I RCCGGY 1 cut(s) 1073
Cfr13I GGNCC 1 cut(s) 1118
Csp6I GTAC 1 cut(s) 933
CviAII CATG 7 cut(s) 98, 295, 329, 347, 650, 1016, 1103
CviQI GTAC 1 cut(s) 933
DdeI CTNAG 3 cut(s) 589, 633, 972
DpnI GATC 4 cut(s) 22, 203, 701, 750
DpnII GATC 4 cut(s) 20, 201, 699, 748
EaeI YGGCCR 1 cut(s) 1071
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Ecl136II GAGCTC 2 cut(s) 190, 835
Eco130I CCWWGG 2 cut(s) 718, 1102
Eco24I GRGCYC 2 cut(s) 192, 837
Eco47I GGWCC 1 cut(s) 1118
Eco53kI GAGCTC 2 cut(s) 190, 835
EcoICRI GAGCTC 2 cut(s) 190, 835
EcoT14I CCWWGG 2 cut(s) 718, 1102
EcoT38I GRGCYC 2 cut(s) 192, 837
ErhI CCWWGG 2 cut(s) 718, 1102
FaeI CATG 7 cut(s) 101, 298, 332, 350, 653, 1019, 1106
FalI AAGNNNNNCTT 2 cut(s) 910, 942
FaqI GGGAC 1 cut(s) 1011
FatI CATG 7 cut(s) 97, 294, 328, 346, 649, 1015, 1102
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 2 cut(s) 741, 810
FokI GGATG 4 cut(s) 100, 483, 558, 686
FriOI GRGCYC 2 cut(s) 192, 837
Fsp4HI GCNGC 2 cut(s) 741, 810
FspBI CTAG 4 cut(s) 30, 618, 725, 1130
GlaI GCGC 1 cut(s) 263
GluI GCNGC 2 cut(s) 741, 810
GsaI CCCAGC 1 cut(s) 262
GsuI CTGGAG 3 cut(s) 206, 272, 672
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 2 cut(s) 723, 1073
HapII CCGG 2 cut(s) 310, 1074
HhaI GCGC 1 cut(s) 264
Hin1II CATG 7 cut(s) 101, 298, 332, 350, 653, 1019, 1106
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 4 cut(s) 209, 313, 337, 593
HpaII CCGG 2 cut(s) 310, 1074
HphI GGTGA 1 cut(s) 246
Hpy166II GTNNAC 2 cut(s) 597, 1003
Hpy188I TCNGA 1 cut(s) 1110
Hpy188III TCNNGA 7 cut(s) 85, 141, 185, 329, 407, 905, 1080
Hpy8I GTNNAC 2 cut(s) 597, 1003
Hpy99I CGWCG 1 cut(s) 147
HpyCH4III ACNGT 3 cut(s) 528, 644, 1114
HpyCH4IV ACGT 1 cut(s) 145
HpyF10VI GCNNNNNNNGC 1 cut(s) 809
HpyF3I CTNAG 3 cut(s) 589, 633, 972
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 7 cut(s) 101, 298, 332, 350, 653, 1019, 1106
HspAI GCGC 1 cut(s) 262
KpnI GGTACC 1 cut(s) 936
Ksp22I TGATCA 1 cut(s) 748
Kzo9I GATC 4 cut(s) 20, 201, 699, 748
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 3 cut(s) 187, 832, 989
Lsp1109I GCAGC 2 cut(s) 727, 821
LweI GCATC 3 cut(s) 430, 664, 858
MaeI CTAG 4 cut(s) 30, 618, 725, 1130
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 1 cut(s) 644
MalI GATC 4 cut(s) 22, 203, 701, 750
MboI GATC 4 cut(s) 20, 201, 699, 748
MboII GAAGA 8 cut(s) 73, 100, 177, 184, 218, 243, 521, 883
MfeI CAATTG 1 cut(s) 222
MflI RGATCY 2 cut(s) 20, 699
MhlI GDGCHC 3 cut(s) 192, 393, 837
MluCI AATT 7 cut(s) 222, 282, 460, 513, 666, 957, 966
MlyI GAGTC 1 cut(s) 602
MmeI TCCRAC 1 cut(s) 1096
MnlI CCTC 6 cut(s) 192, 207, 493, 642, 769, 947
MseI TTAA 2 cut(s) 395, 446
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 2 cut(s) 310, 1074
MunI CAATTG 1 cut(s) 222
MwoI GCNNNNNNNGC 1 cut(s) 809
NcoI CCATGG 1 cut(s) 1102
NdeII GATC 4 cut(s) 20, 201, 699, 748
NheI GCTAGC 1 cut(s) 617
NlaIII CATG 7 cut(s) 101, 298, 332, 350, 653, 1019, 1106
NlaIV GGNNCC 2 cut(s) 308, 934
NmuCI GTSAC 1 cut(s) 644
NspI RCATGY 1 cut(s) 1019
PaeI GCATGC 1 cut(s) 1019
PagI TCATGA 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 3 cut(s) 209, 313, 337
PflFI GACNNNGTC 1 cut(s) 552
PkrI GCNGC 2 cut(s) 742, 811
Ple19I CGATCG 1 cut(s) 204
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
Psp124BI GAGCTC 2 cut(s) 192, 837
PspFI CCCAGC 1 cut(s) 258
PspN4I GGNNCC 2 cut(s) 308, 934
PspPI GGNCC 1 cut(s) 1118
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 2 cut(s) 20, 699
PsyI GACNNNGTC 1 cut(s) 552
PvuI CGATCG 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 1 cut(s) 934
RsaNI GTAC 1 cut(s) 933
SacI GAGCTC 2 cut(s) 192, 837
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 395, 446
SatI GCNGC 2 cut(s) 741, 810
Sau3AI GATC 4 cut(s) 20, 201, 699, 748
Sau96I GGNCC 1 cut(s) 1118
SchI GAGTC 1 cut(s) 602
SduI GDGCHC 3 cut(s) 192, 393, 837
SfaNI GCATC 3 cut(s) 430, 664, 858
SinI GGWCC 1 cut(s) 1118
SmlI CTYRAG 1 cut(s) 85
SmoI CTYRAG 1 cut(s) 85
SphI GCATGC 1 cut(s) 1019
Sse9I AATT 7 cut(s) 222, 282, 460, 513, 666, 957, 966
SspMI CTAG 4 cut(s) 30, 618, 725, 1130
SstI GAGCTC 2 cut(s) 192, 837
StyI CCWWGG 2 cut(s) 718, 1102
TaaI ACNGT 3 cut(s) 528, 644, 1114
TaiI ACGT 1 cut(s) 148
TaqI TCGA 6 cut(s) 75, 142, 204, 212, 507, 1096
TasI AATT 7 cut(s) 222, 282, 460, 513, 666, 957, 966
TfiI GAWTC 3 cut(s) 209, 313, 337
Tru1I TTAA 2 cut(s) 395, 446
Tru9I TTAA 2 cut(s) 395, 446
TscAI CASTG 4 cut(s) 296, 531, 696, 980
TseFI GTSAC 1 cut(s) 644
TseI GCWGC 2 cut(s) 740, 809
Tsp45I GTSAC 1 cut(s) 644
TspDTI ATGAA 6 cut(s) 114, 285, 311, 317, 507, 870
TspGWI ACGGA 2 cut(s) 533, 686
TspRI CASTG 4 cut(s) 296, 531, 696, 980
Tth111I GACNNNGTC 1 cut(s) 552
VpaK11BI GGWCC 1 cut(s) 1118
XapI RAATTY 2 cut(s) 282, 966
XceI RCATGY 1 cut(s) 1019
XspI CTAG 4 cut(s) 30, 618, 725, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.