Rw6G008540

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000508
Physical Location & Seq
Forward (+)
14420272 .. 14421605
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G008540.1

Sequence Viewer

Length: 813 bp
ATGTATAGAAGGCAGACAAAAGAAAGTTCAATGAACAAGCCGACTATGGAGAGCCAAGAAGCTTGTGTGATTGAGATCCGACCTTCTTTTTCTTCAAAGAGGGCTTTGGAGAATTGGAGGAAGCTATCTGCTGGCCTCAAGAAGCCCAACAAGCGACAGTTTCTCTTCAACCCTGCGGCTCGCGCCCTTTGGATTAAGGAGCAGGAGGAACACATTCAGACCGTATTGCAATTTGTGAATGCTCGCCAGGAGTCTAACAGCAGGTTGTCTTTGCTTGGCAGTAGTTCCGGTGACGAGTCAAGGCACACAACAAAGGCTACTTCAACAGTATGGGTCATTTTACTCACCAACCTCGGCCTAGAGACATTATCGGCCGTCTTTGATCAAATTGCCTCTCCAAGCAAGCCTGACTATGCACTATTCGGAATGGTGTTGGCGATTTTAGCTGTGCTAATTTGCATCTTGGAGCTCATTCACAAGGGCAAAAAGGAAGGAGTTGAGCTGAAAAGATGGGGAAAGTTGTGCTGGTTTTATTATCCACCTCCTAATGAATCACTTTTTGGTACTTTCCCAGATATTTGTGGATTAGTCATTGCCATCTCACAGTATATTTGCTCCACTGTTCAGTATGTTTATCTGTGCAGGCATGCTAATAACCCCATCAAACTATCCGTTTTGCCGGCCATCTTTCTTATTTGTTTGGCTGGTTCAAAACTAATTAGGAACAGAAGCTACACCACAAATGAGACCTTTAAACATACTACTCATCATATTACAAAGATTAATTTTTGTAGATTTTGGCATTCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

30.81

Weight (kDa)

9.53

Isoelectric Point (pI)

54.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AccII CGCG 1 cut(s) 183
AciI CCGC 1 cut(s) 176
AclWI GGATC 1 cut(s) 70
AcoI YGGCCR 2 cut(s) 372, 681
AfaI GTAC 1 cut(s) 565
AgsI TTSAA 5 cut(s) 30, 96, 169, 324, 711
AjnI CCWGG 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AluBI AGCT 6 cut(s) 62, 124, 446, 469, 502, 732
AluI AGCT 6 cut(s) 62, 124, 446, 469, 502, 732
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 2 cut(s) 356, 740
AlwI GGATC 1 cut(s) 70
AoxI GGCC 4 cut(s) 133, 355, 372, 681
AseI ATTAAT 1 cut(s) 783
Asp700I GAANNNNTTC 1 cut(s) 213
AspLEI GCGC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 302, 337
BanII GRGCYC 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 471
BccI CCATC 4 cut(s) 504, 605, 668, 692
BceAI ACGGC 1 cut(s) 359
BciT130I CCWGG 1 cut(s) 248
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 2 cut(s) 356, 740
BfaI CTAG 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 1 cut(s) 352
BsaWI WCCGGW 1 cut(s) 287
Bse118I RCCGGY 1 cut(s) 679
Bse3DI GCAATG 1 cut(s) 591
Bse8I GATNNNNATC 1 cut(s) 74
BseBI CCWGG 1 cut(s) 248
BseDI CCNNGG 1 cut(s) 352
BseJI GATNNNNATC 1 cut(s) 74
BseMI GCAATG 1 cut(s) 591
BseX3I CGGCCG 1 cut(s) 372
BsgI GTGCAG 1 cut(s) 661
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 4 cut(s) 135, 357, 374, 683
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 471
BsiSI CCGG 2 cut(s) 288, 680
BsmAI GTCTC 2 cut(s) 356, 740
BsmI GAATGC 2 cut(s) 244, 802
BsnI GGCC 4 cut(s) 135, 357, 374, 683
Bso31I GGTCTC 1 cut(s) 740
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 2 cut(s) 75, 382
BspACI CCGC 1 cut(s) 176
BspANI GGCC 4 cut(s) 135, 357, 374, 683
BspFNI CGCG 1 cut(s) 183
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 740
BsrDI GCAATG 1 cut(s) 591
BsrFI RCCGGY 1 cut(s) 679
BssAI RCCGGY 1 cut(s) 679
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 2 cut(s) 75, 382
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 5 cut(s) 159, 223, 328, 606, 622
Bst6I CTCTTC 1 cut(s) 170
BstC8I GCNNGC 7 cut(s) 133, 181, 244, 404, 644, 648, 681
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 2 cut(s) 78, 385
BstMAI GTCTC 2 cut(s) 356, 740
BstMBI GATC 2 cut(s) 75, 382
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 3 cut(s) 151, 182, 443
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 1 cut(s) 246
BstUI CGCG 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BstZI CGGCCG 1 cut(s) 372
BsuRI GGCC 4 cut(s) 135, 357, 374, 683
BtsIMutI CAGTG 1 cut(s) 618
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 7 cut(s) 133, 181, 244, 404, 644, 648, 681
CfoI GCGC 1 cut(s) 185
Cfr10I RCCGGY 1 cut(s) 679
Csp6I GTAC 1 cut(s) 564
CviAII CATG 1 cut(s) 647
CviQI GTAC 1 cut(s) 564
DpnI GATC 2 cut(s) 77, 384
DpnII GATC 2 cut(s) 75, 382
DraI TTTAAA 1 cut(s) 754
EaeI YGGCCR 2 cut(s) 372, 681
EagI CGGCCG 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 469
EclXI CGGCCG 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 471
Eco31I GGTCTC 1 cut(s) 740
Eco52I CGGCCG 1 cut(s) 372
Eco53kI GAGCTC 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 471
FaeI CATG 1 cut(s) 650
FaiI YATR 9 cut(s) 6, 47, 331, 414, 609, 630, 648, 759, 771
FatI CATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 1 cut(s) 177
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 1 cut(s) 359
GlaI GCGC 1 cut(s) 184
GluI GCNGC 1 cut(s) 177
HaeIII GGCC 4 cut(s) 135, 357, 374, 683
HapII CCGG 2 cut(s) 288, 680
HhaI GCGC 1 cut(s) 185
Hin1II CATG 1 cut(s) 650
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HindIII AAGCTT 1 cut(s) 60
HinfI GANTC 3 cut(s) 251, 296, 551
HpaII CCGG 2 cut(s) 288, 680
HphI GGTGA 2 cut(s) 302, 337
Hpy188I TCNGA 3 cut(s) 80, 219, 425
Hpy188III TCNNGA 1 cut(s) 139
HpyAV CCTTC 3 cut(s) 3, 93, 485
HpyCH4III ACNGT 5 cut(s) 159, 223, 328, 606, 622
HpyCH4V TGCA 4 cut(s) 229, 416, 459, 642
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 182, 443
Hsp92II CATG 1 cut(s) 650
HspAI GCGC 1 cut(s) 183
KroI GCCGGC 1 cut(s) 679
KroNI GCCGGC 1 cut(s) 681
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 2 cut(s) 75, 382
LmnI GCTCC 3 cut(s) 199, 466, 620
LweI GCATC 1 cut(s) 468
MaeI CTAG 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 290
MalI GATC 2 cut(s) 77, 384
MboI GATC 2 cut(s) 75, 382
MboII GAAGA 2 cut(s) 84, 157
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 6 cut(s) 112, 230, 387, 453, 717, 784
MlyI GAGTC 2 cut(s) 260, 305
MmeI TCCRAC 1 cut(s) 103
MnlI CCTC 7 cut(s) 93, 111, 146, 199, 362, 403, 552
MroNI GCCGGC 1 cut(s) 679
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 3 cut(s) 195, 753, 783
MspI CCGG 2 cut(s) 288, 680
MspR9I CCNGG 1 cut(s) 248
Mva1269I GAATGC 2 cut(s) 244, 802
MvaI CCWGG 1 cut(s) 248
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 3 cut(s) 151, 182, 443
NaeI GCCGGC 1 cut(s) 681
NdeII GATC 2 cut(s) 75, 382
NgoMIV GCCGGC 1 cut(s) 679
NlaIII CATG 1 cut(s) 650
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 290
NspI RCATGY 1 cut(s) 650
PaeI GCATGC 1 cut(s) 650
PctI GAATGC 2 cut(s) 244, 802
PdiI GCCGGC 1 cut(s) 681
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 551
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 2 cut(s) 259, 304
PpsI GAGTC 2 cut(s) 259, 304
PshBI ATTAAT 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 471
Psp6I CCWGG 1 cut(s) 246
PspGI CCWGG 1 cut(s) 246
PsrI GAACNNNNNNTAC 2 cut(s) 716, 748
PsuI RGATCY 1 cut(s) 75
RsaI GTAC 1 cut(s) 565
RsaNI GTAC 1 cut(s) 564
SacI GAGCTC 1 cut(s) 471
SaqAI TTAA 3 cut(s) 195, 753, 783
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 2 cut(s) 75, 382
SchI GAGTC 2 cut(s) 260, 305
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SphI GCATGC 1 cut(s) 650
Sse9I AATT 6 cut(s) 112, 230, 387, 453, 717, 784
SsiI CCGC 1 cut(s) 176
SspMI CTAG 1 cut(s) 359
SstI GAGCTC 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 5 cut(s) 159, 223, 328, 606, 622
TasI AATT 6 cut(s) 112, 230, 387, 453, 717, 784
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 1 cut(s) 551
Tru1I TTAA 3 cut(s) 195, 753, 783
Tru9I TTAA 3 cut(s) 195, 753, 783
TscAI CASTG 1 cut(s) 625
TseFI GTSAC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 2 cut(s) 47, 564
TspGWI ACGGA 1 cut(s) 661
TspRI CASTG 1 cut(s) 625
VspI ATTAAT 1 cut(s) 783
XceI RCATGY 1 cut(s) 650
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.