Prupe.5G012300_v2.0.a1

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000508
Physical Location & Seq
Reverse (-)
1332385 .. 1334949
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G012300.1

Sequence Viewer

Length: 840 bp
ATGAGGCCCAATTCAGAATCAGCAGAGTGGATATTTTTAGCCACTAACCTTGGCCTAGAGATGTTGTCAGCTGCTTGTGATCAGGCTTCCTCCCCAAGAAGACCCCACTTTGCACTATATGGGATGCTGTTGGCTATTGCAGCTGTGCTCATTTCCATTGGGGAGCTAATTTATAGAGGTCAACAGGAAAACGTTGAGTTCAGGAGCCGGGGAATGCGCTCGTGGTTTTATCATCCACCAATTCCTCCATATACGCCTTTTGGTACTCTTCCTGACATTTTTGGACTAGTTGCTGGCATCTCACAGTGCATTTGCTCTATAGTTCAGTATGTTTACTGCCTTCGGCATGCTGATAGTCCTTTGAAAGCATCCCTTTTGCCTGCCATATTTCTTATCTGTTTAGTTGGTTCAAGACTAAGTAATAAACGAATGAACGCCAATACCACTGATAACATGGACTTGTCGGAAAATTCTTCAAGCACGGAAGAAACTTCGTTGCATCCAATCCCGGAGTATTGTGCACCTGCAAATATACCATTTCTCGATAATCAGGAGGTAGAGGTTGTGAGCAACATAGTGCAGCAATGGCGGTGGATGCGGGAGCGGGAGCGGGAGCGGGAGCAGGAGCGGGAGTGGGAGCTGGAGTGGGAGCGGGAGCAGGAGCGCGAGAGGGTGCAGGAGCGGGAGCTGCGGCGGATGTGGGAGCATCTGGAGCAGCTACCAGAGCCGCTGCTATGGGAGAAGCTATCGGGGCTACCAAAGCGGGAGCCGAAGCAGCTGCAGCTATCGAAGGCCCCCCTAAGGCTCCGGCGGGTTTCGCGGGTAGTGATCGCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

32.46

Weight (kDa)

5.71

Isoelectric Point (pI)

61.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 532
Acc36I ACCTGC 1 cut(s) 532
AccBSI CCGCTC 6 cut(s) 604, 610, 616, 628, 652, 682
AccII CGCG 3 cut(s) 666, 820, 833
AclI AACGTT 1 cut(s) 192
AcsI RAATTY 1 cut(s) 469
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 1 cut(s) 801
AgsI TTSAA 3 cut(s) 364, 411, 477
AhlI ACTAGT 1 cut(s) 286
AluBI AGCT 9 cut(s) 71, 143, 166, 640, 688, 718, 745, 778, 784
AluI AGCT 9 cut(s) 71, 143, 166, 640, 688, 718, 745, 778, 784
Alw21I GWGCWC 2 cut(s) 150, 523
Alw44I GTGCAC 1 cut(s) 519
AoxI GGCC 3 cut(s) 5, 52, 792
ApaLI GTGCAC 1 cut(s) 519
ApeKI GCWGC 9 cut(s) 71, 140, 580, 688, 715, 730, 775, 778, 781
ApoI RAATTY 1 cut(s) 469
AspLEI GCGC 2 cut(s) 219, 666
AspS9I GGNCC 2 cut(s) 6, 793
AsuC2I CCSGG 2 cut(s) 209, 509
AxyI CCTNAGG 1 cut(s) 800
BaeGI GKGCMC 1 cut(s) 523
BauI CACGAG 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 106
Bbv12I GWGCWC 2 cut(s) 150, 523
BbvI GCAGC 9 cut(s) 58, 152, 592, 675, 717, 727, 765, 787, 793
BcgI CGANNNNNNTGC 2 cut(s) 760, 794
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 2 cut(s) 209, 509
BcuI ACTAGT 1 cut(s) 286
BfaI CTAG 2 cut(s) 56, 287
BfmI CTRYAG 2 cut(s) 318, 779
BfuAI ACCTGC 1 cut(s) 532
Bme1390I CCNGG 2 cut(s) 209, 509
BmgT120I GGNCC 2 cut(s) 6, 793
BmiI GGNNCC 4 cut(s) 206, 768, 795, 806
BmrFI CCNGG 2 cut(s) 209, 509
BmsI GCATC 6 cut(s) 114, 306, 377, 508, 585, 715
BpiI GAAGAC 1 cut(s) 106
BpmI CTGGAG 2 cut(s) 662, 731
BpuMI CCSGG 2 cut(s) 209, 509
BsaJI CCNNGG 2 cut(s) 49, 208
BsaXI ACNNNNNCTCC 4 cut(s) 617, 629, 647, 659
Bsc4I CCNNNNNNNGG 1 cut(s) 801
Bse21I CCTNAGG 1 cut(s) 800
Bse3DI GCAATG 1 cut(s) 590
BseDI CCNNGG 2 cut(s) 49, 208
BseGI GGATG 6 cut(s) 129, 232, 368, 499, 600, 702
BseLI CCNNNNNNNGG 1 cut(s) 801
BseMI GCAATG 1 cut(s) 590
BseSI GKGCMC 1 cut(s) 523
BseXI GCAGC 9 cut(s) 58, 152, 592, 675, 717, 727, 765, 787, 793
BsgI GTGCAG 2 cut(s) 599, 695
Bsh1236I CGCG 3 cut(s) 666, 820, 833
BshFI GGCC 3 cut(s) 7, 54, 794
BsiHKAI GWGCWC 2 cut(s) 150, 523
BsiSI CCGG 3 cut(s) 208, 509, 808
BslI CCNNNNNNNGG 1 cut(s) 801
BsmI GAATGC 1 cut(s) 219
BsnI GGCC 3 cut(s) 7, 54, 794
Bsp1286I GDGCHC 2 cut(s) 150, 523
Bsp143I GATC 2 cut(s) 79, 828
BspANI GGCC 3 cut(s) 7, 54, 794
BspFNI CGCG 3 cut(s) 666, 820, 833
BspLI GGNNCC 4 cut(s) 206, 768, 795, 806
BspMAI CTGCAG 1 cut(s) 783
BspMI ACCTGC 1 cut(s) 532
BsrBI CCGCTC 6 cut(s) 604, 610, 616, 628, 652, 682
BsrDI GCAATG 1 cut(s) 590
BssECI CCNNGG 2 cut(s) 49, 208
BssMI GATC 2 cut(s) 79, 828
BssSI CACGAG 1 cut(s) 220
BssT1I CCWWGG 1 cut(s) 49
Bst2BI CACGAG 1 cut(s) 220
Bst4CI ACNGT 1 cut(s) 306
Bst6I CTCTTC 1 cut(s) 273
BstC8I GCNNGC 3 cut(s) 295, 348, 381
BstDEI CTNAG 2 cut(s) 416, 800
BstF5I GGATG 6 cut(s) 129, 232, 368, 499, 600, 702
BstFNI CGCG 3 cut(s) 666, 820, 833
BstHHI GCGC 2 cut(s) 219, 666
BstKTI GATC 2 cut(s) 82, 831
BstMBI GATC 2 cut(s) 79, 828
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 2 cut(s) 207, 507
BstSFI CTRYAG 2 cut(s) 318, 779
BstSLI GKGCMC 1 cut(s) 523
BstUI CGCG 3 cut(s) 666, 820, 833
BstV1I GCAGC 9 cut(s) 58, 152, 592, 675, 717, 727, 765, 787, 793
BstV2I GAAGAC 1 cut(s) 106
Bsu36I CCTNAGG 1 cut(s) 800
BsuRI GGCC 3 cut(s) 7, 54, 794
BtsCI GGATG 6 cut(s) 129, 232, 368, 499, 600, 702
BtsIMutI CAGTG 2 cut(s) 311, 444
BveI ACCTGC 1 cut(s) 532
Cac8I GCNNGC 3 cut(s) 295, 348, 381
CfoI GCGC 2 cut(s) 219, 666
Cfr13I GGNCC 2 cut(s) 6, 793
Csp6I GTAC 1 cut(s) 264
CspCI CAANNNNNGTGG 4 cut(s) 31, 66, 572, 607
CviAII CATG 2 cut(s) 347, 454
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 2 cut(s) 416, 800
DpnI GATC 2 cut(s) 81, 830
DpnII GATC 2 cut(s) 79, 828
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
EciI GGCGGA 1 cut(s) 709
Eco130I CCWWGG 1 cut(s) 49
Eco81I CCTNAGG 1 cut(s) 800
EcoO109I RGGNCCY 1 cut(s) 793
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 350, 457
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FatI CATG 2 cut(s) 346, 453
FbaI TGATCA 1 cut(s) 79
FokI GGATG 6 cut(s) 136, 219, 355, 486, 607, 709
FspBI CTAG 2 cut(s) 56, 287
GlaI GCGC 2 cut(s) 218, 665
GsuI CTGGAG 2 cut(s) 662, 731
HaeIII GGCC 3 cut(s) 7, 54, 794
HapII CCGG 3 cut(s) 208, 509, 808
HhaI GCGC 2 cut(s) 219, 666
Hin1II CATG 2 cut(s) 350, 457
Hin6I GCGC 2 cut(s) 217, 664
HinP1I GCGC 2 cut(s) 217, 664
HincII GTYRAC 1 cut(s) 182
HindII GTYRAC 1 cut(s) 182
HinfI GANTC 1 cut(s) 17
HpaII CCGG 3 cut(s) 208, 509, 808
Hpy166II GTNNAC 3 cut(s) 182, 334, 521
Hpy188I TCNGA 2 cut(s) 16, 466
Hpy188III TCNNGA 6 cut(s) 202, 272, 411, 542, 551, 710
Hpy8I GTNNAC 3 cut(s) 182, 334, 521
HpyAV CCTTC 2 cut(s) 350, 784
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 9 cut(s) 113, 140, 309, 499, 521, 527, 580, 676, 781
HpyF3I CTNAG 2 cut(s) 416, 800
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 2 cut(s) 350, 457
HspAI GCGC 2 cut(s) 217, 664
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 2 cut(s) 79, 828
Lsp1109I GCAGC 9 cut(s) 58, 152, 592, 675, 717, 727, 765, 787, 793
LweI GCATC 6 cut(s) 114, 306, 377, 508, 585, 715
MaeI CTAG 2 cut(s) 56, 287
MaeII ACGT 1 cut(s) 192
MalI GATC 2 cut(s) 81, 830
MbiI CCGCTC 6 cut(s) 604, 610, 616, 628, 652, 682
MboI GATC 2 cut(s) 79, 828
MboII GAAGA 4 cut(s) 111, 260, 465, 497
MhlI GDGCHC 2 cut(s) 150, 523
MluCI AATT 4 cut(s) 10, 168, 240, 469
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 6 cut(s) 100, 170, 255, 547, 553, 663
MspA1I CMGCKG 4 cut(s) 71, 143, 730, 778
MspI CCGG 3 cut(s) 208, 509, 808
MspR9I CCNGG 2 cut(s) 209, 509
Mva1269I GAATGC 1 cut(s) 219
MvnI CGCG 3 cut(s) 666, 820, 833
NciI CCSGG 2 cut(s) 209, 509
NdeII GATC 2 cut(s) 79, 828
NlaIII CATG 2 cut(s) 350, 457
NlaIV GGNNCC 4 cut(s) 206, 768, 795, 806
NspI RCATGY 1 cut(s) 350
PaeI GCATGC 1 cut(s) 350
PaqCI CACCTGC 1 cut(s) 532
PctI GAATGC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 17
PfoI TCCNGGA 1 cut(s) 507
Psp1406I AACGTT 1 cut(s) 192
PspN4I GGNNCC 4 cut(s) 206, 768, 795, 806
PspPI GGNCC 2 cut(s) 6, 793
PstI CTGCAG 1 cut(s) 783
PvuII CAGCTG 3 cut(s) 71, 143, 778
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
Sau3AI GATC 2 cut(s) 79, 828
Sau96I GGNCC 2 cut(s) 6, 793
ScrFI CCNGG 2 cut(s) 209, 509
SduI GDGCHC 2 cut(s) 150, 523
SfaNI GCATC 6 cut(s) 114, 306, 377, 508, 585, 715
SfcI CTRYAG 2 cut(s) 318, 779
SpeI ACTAGT 1 cut(s) 286
SphI GCATGC 1 cut(s) 350
Sse9I AATT 4 cut(s) 10, 168, 240, 469
SspMI CTAG 2 cut(s) 56, 287
StyD4I CCNGG 2 cut(s) 207, 507
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 306
TaiI ACGT 1 cut(s) 195
TaqI TCGA 2 cut(s) 543, 788
TasI AATT 4 cut(s) 10, 168, 240, 469
TauI GCSGC 2 cut(s) 694, 730
TfiI GAWTC 1 cut(s) 17
TscAI CASTG 2 cut(s) 311, 451
TseI GCWGC 9 cut(s) 71, 140, 580, 688, 715, 730, 775, 778, 781
TspDTI ATGAA 1 cut(s) 446
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 2 cut(s) 311, 451
VneI GTGCAC 1 cut(s) 519
XapI RAATTY 1 cut(s) 469
XceI RCATGY 1 cut(s) 350
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XspI CTAG 2 cut(s) 56, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.