Rroxscaffold_7G00192440

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000508
Physical Location & Seq
Forward (+)
33637968 .. 33649205
11238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00192440.1

Sequence Viewer

Length: 1011 bp
ATGGTGTTGAACAAGCAGAGAGTGTCTATTCCATTACGGATATCTATTCCTTTTACTTCTCTATGGGCGTGTGATGTCTTCCTAAGTTGCGAGCATGAAGACATCTTCTCCGACCACTTGTACTACAAATTGCAGTCTCGAGGAATTAGTATTTTCCAGGATGGCCAAGGATTGCAAAGAAACACAACTCCTTTTGAGCTCTTTGGTGCAATCGAAGAATCGAGGTTTGCCGTGATTGTTCTCTCACAAAATTATGCTTCTTCGCCCTGGCGCTTGAATGAACTTTCAAAGATTCTAGAGTGCATGAAAGATCGGAACAGGATTCTGCCGGTATTTCACGATGTTAATCTCTTGGATGTCCAAAAACAAAAAGGTACTTTTGAGAAAGCATTTGACAAGCATGAAGAAAGGTTTCAGGATGACTTGGAAAAGTTTCAAGCCTGGAGAGATGCTTTAACCAAAGTGTGCAATTTTGCTGGATGGACTACCAATGACAGGAATGAAGTACAGGTTATAGAAGAAATTACTGAAGCACTGTGGAACAAACTACATTCTAGAATCAGTCCAACGGAAAAACTGCAGCCGACATCCACACACAACTTGGTCGCATTCACTAGCATTAGTGTTAATGAGGACAACCATGACCATAACCATCCAATAAACATTACAGATGTGACGGCTACTTCAGTAGAGTGGAAGTTTTTGATTACTAACCTTGGCCTAGAGATTTTGTCAGCTGCTTTTGATCAGGCTTCCTCGTCTACTAAGCCACACTATGCACTATTTGGTATGCTGTTTTCTATTGCGGCCTTGTTCACTTGCATTTGGGAGCTCATTTACAAGGGCATAAAGAATAGAGTAGTATTGAAGAAGTTCGGAAAGCTCTGGTGGTTTTATTATCCACATCCCCACAATAGCATGCCTTTCGGTACTCTCCCTGAGATTTACGGATTAATTGGAAGCATCGCTCAGCACCAAAAGTTTCTTTCAAGAAGCCCATTGGAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

336

Amino Acids

38.84

Weight (kDa)

6.36

Isoelectric Point (pI)

54.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 187 9.4e-34 TIR domain
TIR_2 PF13676 26 - 117 2.2e-12 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 761
AciI CCGC 1 cut(s) 806
AcoI YGGCCR 1 cut(s) 163
AcuI CTGAAG 2 cut(s) 549, 669
AfaI GTAC 4 cut(s) 122, 376, 507, 931
AfiI CCNNNNNNNGG 1 cut(s) 495
AgsI TTSAA 6 cut(s) 10, 277, 288, 437, 868, 990
AjnI CCWGG 3 cut(s) 156, 266, 440
AluBI AGCT 4 cut(s) 199, 737, 832, 883
AluI AGCT 4 cut(s) 199, 737, 832, 883
Alw21I GWGCWC 2 cut(s) 201, 834
Alw26I GTCTC 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 3 cut(s) 163, 718, 807
ApeKI GCWGC 2 cut(s) 580, 737
AseI ATTAAT 1 cut(s) 953
Asp700I GAANNNNTTC 3 cut(s) 411, 432, 872
AspLEI GCGC 1 cut(s) 273
AvaI CYCGRG 1 cut(s) 138
BalI TGGCCA 1 cut(s) 165
BanII GRGCYC 2 cut(s) 201, 834
BbsI GAAGAC 2 cut(s) 70, 105
Bbv12I GWGCWC 2 cut(s) 201, 834
BbvI GCAGC 2 cut(s) 592, 724
BccI CCATC 3 cut(s) 155, 474, 660
BceAI ACGGC 2 cut(s) 215, 693
BcgI CGANNNNNNTGC 2 cut(s) 907, 941
BciT130I CCWGG 3 cut(s) 158, 268, 442
BclI TGATCA 1 cut(s) 745
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 4 cut(s) 296, 555, 615, 722
BfmI CTRYAG 1 cut(s) 578
BfoI RGCGCY 1 cut(s) 274
BisI GCNGC 3 cut(s) 581, 738, 807
BlpI GCTNAGC 1 cut(s) 969
BlsI GCNGC 3 cut(s) 582, 739, 808
Bme1390I CCNGG 3 cut(s) 158, 268, 442
BmeT110I CYCGRG 1 cut(s) 138
BmrFI CCNGG 3 cut(s) 158, 268, 442
BmsI GCATC 2 cut(s) 439, 972
BpiI GAAGAC 2 cut(s) 70, 105
BpmI CTGGAG 1 cut(s) 463
Bpu1102I GCTNAGC 1 cut(s) 969
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 3 cut(s) 166, 266, 715
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 1 cut(s) 495
Bse118I RCCGGY 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 3 cut(s) 158, 268, 442
BseDI CCNNGG 3 cut(s) 166, 266, 715
BseGI GGATG 7 cut(s) 166, 361, 424, 485, 587, 652, 904
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 495
BseMII CTCAG 2 cut(s) 930, 983
BseXI GCAGC 2 cut(s) 592, 724
BshFI GGCC 3 cut(s) 165, 720, 809
BsiHKAI GWGCWC 2 cut(s) 201, 834
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 1 cut(s) 329
BslI CCNNNNNNNGG 1 cut(s) 495
BsmAI GTCTC 1 cut(s) 141
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 3 cut(s) 165, 720, 809
BsoBI CYCGRG 1 cut(s) 138
Bsp1286I GDGCHC 2 cut(s) 201, 834
Bsp143I GATC 2 cut(s) 310, 745
Bsp1720I GCTNAGC 1 cut(s) 969
BspACI CCGC 1 cut(s) 806
BspANI GGCC 3 cut(s) 165, 720, 809
BspCNI CTCAG 2 cut(s) 931, 982
BspMAI CTGCAG 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 328
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 3 cut(s) 166, 266, 715
BssMI GATC 2 cut(s) 310, 745
BssT1I CCWWGG 2 cut(s) 166, 715
Bst2UI CCWGG 3 cut(s) 158, 268, 442
Bst4CI ACNGT 1 cut(s) 537
BstC8I GCNNGC 2 cut(s) 92, 920
BstDEI CTNAG 4 cut(s) 83, 765, 939, 969
BstF5I GGATG 7 cut(s) 166, 361, 424, 485, 587, 652, 904
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 2 cut(s) 313, 748
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 2 cut(s) 310, 745
BstNI CCWGG 3 cut(s) 158, 268, 442
BstNSI RCATGY 1 cut(s) 922
BstSCI CCNGG 3 cut(s) 156, 266, 440
BstSFI CTRYAG 1 cut(s) 578
BstV1I GCAGC 2 cut(s) 592, 724
BstV2I GAAGAC 2 cut(s) 70, 105
BsuRI GGCC 3 cut(s) 165, 720, 809
BtgZI GCGATG 1 cut(s) 949
BtsCI GGATG 7 cut(s) 166, 361, 424, 485, 587, 652, 904
BtsIMutI CAGTG 1 cut(s) 533
Cac8I GCNNGC 2 cut(s) 92, 920
CfoI GCGC 1 cut(s) 273
Cfr10I RCCGGY 1 cut(s) 328
Csp6I GTAC 4 cut(s) 121, 375, 506, 930
CviAII CATG 5 cut(s) 95, 304, 401, 641, 919
CviQI GTAC 4 cut(s) 121, 375, 506, 930
DdeI CTNAG 4 cut(s) 83, 765, 939, 969
DpnI GATC 2 cut(s) 312, 747
DpnII GATC 2 cut(s) 310, 745
EaeI YGGCCR 1 cut(s) 163
Ecl136II GAGCTC 2 cut(s) 199, 832
Eco130I CCWWGG 2 cut(s) 166, 715
Eco24I GRGCYC 2 cut(s) 201, 834
Eco32I GATATC 1 cut(s) 42
Eco53kI GAGCTC 2 cut(s) 199, 832
Eco57I CTGAAG 2 cut(s) 549, 669
Eco88I CYCGRG 1 cut(s) 138
EcoICRI GAGCTC 2 cut(s) 199, 832
EcoRII CCWGG 3 cut(s) 156, 266, 440
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 2 cut(s) 166, 715
EcoT38I GRGCYC 2 cut(s) 201, 834
ErhI CCWWGG 2 cut(s) 166, 715
FaeI CATG 5 cut(s) 98, 307, 404, 644, 922
FatI CATG 5 cut(s) 94, 303, 400, 640, 918
FbaI TGATCA 1 cut(s) 745
FblI GTMKAC 1 cut(s) 761
Fnu4HI GCNGC 3 cut(s) 581, 738, 807
FokI GGATG 7 cut(s) 173, 368, 431, 492, 574, 639, 891
FriOI GRGCYC 2 cut(s) 201, 834
Fsp4HI GCNGC 3 cut(s) 581, 738, 807
FspBI CTAG 4 cut(s) 296, 555, 615, 722
GlaI GCGC 1 cut(s) 272
GluI GCNGC 3 cut(s) 581, 738, 807
GsuI CTGGAG 1 cut(s) 463
HaeII RGCGCY 1 cut(s) 274
HaeIII GGCC 3 cut(s) 165, 720, 809
HapII CCGG 1 cut(s) 329
HhaI GCGC 1 cut(s) 273
Hin1II CATG 5 cut(s) 98, 307, 404, 644, 922
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HinfI GANTC 4 cut(s) 218, 292, 322, 558
HpaII CCGG 1 cut(s) 329
Hpy166II GTNNAC 2 cut(s) 762, 816
Hpy188I TCNGA 3 cut(s) 112, 315, 878
Hpy188III TCNNGA 6 cut(s) 138, 296, 338, 416, 555, 990
Hpy8I GTNNAC 2 cut(s) 762, 816
HpyCH4III ACNGT 1 cut(s) 537
HpyCH4V TGCA 8 cut(s) 133, 175, 209, 303, 468, 580, 779, 822
HpyF3I CTNAG 4 cut(s) 83, 765, 939, 969
Hsp92II CATG 5 cut(s) 98, 307, 404, 644, 922
HspAI GCGC 1 cut(s) 271
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 2 cut(s) 310, 745
LmnI GCTCC 1 cut(s) 829
Lsp1109I GCAGC 2 cut(s) 592, 724
LweI GCATC 2 cut(s) 439, 972
MaeI CTAG 4 cut(s) 296, 555, 615, 722
MaeIII GTNAC 1 cut(s) 673
MalI GATC 2 cut(s) 312, 747
MboI GATC 2 cut(s) 310, 745
MboII GAAGA 8 cut(s) 70, 97, 110, 227, 252, 416, 530, 880
MhlI GDGCHC 2 cut(s) 201, 834
MlsI TGGCCA 1 cut(s) 165
MluCI AATT 6 cut(s) 128, 144, 250, 469, 522, 954
MluNI TGGCCA 1 cut(s) 165
MmeI TCCRAC 2 cut(s) 135, 590
MnlI CCTC 4 cut(s) 134, 216, 625, 766
Mox20I TGGCCA 1 cut(s) 165
MroXI GAANNNNTTC 3 cut(s) 411, 432, 872
MscI TGGCCA 1 cut(s) 165
MseI TTAA 4 cut(s) 345, 455, 627, 953
Msp20I TGGCCA 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 3 cut(s) 158, 268, 442
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 3 cut(s) 158, 268, 442
NdeII GATC 2 cut(s) 310, 745
NlaIII CATG 5 cut(s) 98, 307, 404, 644, 922
NmuCI GTSAC 1 cut(s) 673
NspI RCATGY 1 cut(s) 922
PaeI GCATGC 1 cut(s) 922
PaeR7I CTCGAG 1 cut(s) 138
PctI GAATGC 1 cut(s) 608
PdmI GAANNNNTTC 3 cut(s) 411, 432, 872
PfeI GAWTC 4 cut(s) 218, 292, 322, 558
PfoI TCCNGGA 1 cut(s) 156
PkrI GCNGC 3 cut(s) 582, 739, 808
PshBI ATTAAT 1 cut(s) 953
Psp124BI GAGCTC 2 cut(s) 201, 834
Psp6I CCWGG 3 cut(s) 156, 266, 440
PspGI CCWGG 3 cut(s) 156, 266, 440
PstI CTGCAG 1 cut(s) 582
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 4 cut(s) 122, 376, 507, 931
RsaNI GTAC 4 cut(s) 121, 375, 506, 930
SacI GAGCTC 2 cut(s) 201, 834
SaqAI TTAA 4 cut(s) 345, 455, 627, 953
SatI GCNGC 3 cut(s) 581, 738, 807
Sau3AI GATC 2 cut(s) 310, 745
ScrFI CCNGG 3 cut(s) 158, 268, 442
SduI GDGCHC 2 cut(s) 201, 834
SetI ASST 9 cut(s) 201, 227, 376, 413, 513, 717, 739, 834, 885
SfaNI GCATC 2 cut(s) 439, 972
SfcI CTRYAG 1 cut(s) 578
Sfr274I CTCGAG 1 cut(s) 138
SlaI CTCGAG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
SphI GCATGC 1 cut(s) 922
Sse9I AATT 6 cut(s) 128, 144, 250, 469, 522, 954
SsiI CCGC 1 cut(s) 806
SspMI CTAG 4 cut(s) 296, 555, 615, 722
SstI GAGCTC 2 cut(s) 201, 834
StyD4I CCNGG 3 cut(s) 156, 266, 440
StyI CCWWGG 2 cut(s) 166, 715
TaaI ACNGT 1 cut(s) 537
TaqI TCGA 3 cut(s) 139, 213, 221
TasI AATT 6 cut(s) 128, 144, 250, 469, 522, 954
TatI WGTACW 2 cut(s) 120, 505
TauI GCSGC 1 cut(s) 809
TfiI GAWTC 4 cut(s) 218, 292, 322, 558
Tru1I TTAA 4 cut(s) 345, 455, 627, 953
Tru9I TTAA 4 cut(s) 345, 455, 627, 953
TscAI CASTG 1 cut(s) 540
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 2 cut(s) 580, 737
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 5 cut(s) 111, 294, 320, 417, 516
TspGWI ACGGA 3 cut(s) 52, 584, 963
TspRI CASTG 1 cut(s) 540
VspI ATTAAT 1 cut(s) 953
XbaI TCTAGA 2 cut(s) 295, 554
XceI RCATGY 1 cut(s) 922
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XhoI CTCGAG 1 cut(s) 138
XmiI GTMKAC 1 cut(s) 761
XmnI GAANNNNTTC 3 cut(s) 411, 432, 872
XspI CTAG 4 cut(s) 296, 555, 615, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.