Rroxscaffold_7G00192480

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000508
Physical Location & Seq
Forward (+)
33666104 .. 33668055
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00192480.1

Sequence Viewer

Length: 570 bp
ATGTACTACTTGAGTACAATTCAATATATGCAGGTCGCATTAACTAGCATTAGTTCGCCTCAGCAGAACCGTGACCATGGCAAGCCAAAGAAAATTACGGATGCAAGGGCTACTCCAGTGGAGTGGATCTTTCTACTCACTAACCTTGGCCTAGAGATTTTGTCAGCTGGTTTTGATCAAGCTTCCTCCCCAAGTAAGCCACACTATGCACTATTTGGGGTGCTGTTTGCTAGCGCAGCTGTTTTTGTTTGCATTTGGGAGCTCATTCACAAGGGCATCAATGAAAAAGTAGTATTGAAGAAGTTTGGAAAGCTATGGTGGTATTATTATCCAGAACCCCACGAAAGCACGCCTTTTGGTACTCTCCCTGACATTTATGGACTAATTGGAGGAATTTCTCAGTGCATATGCTCCATAACTCAATATGTTTACTTCTCTTGGCATGCTGATAGTCCCATAAAACTATCCCTTTTACCTGCCATCTTTCTTTTGTGTTTGGCCGGTTCAAGATTAAACAGAAATCGAAACCATGGTCAGACTGTTGGACCTAAATCCCTAGACAAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

21.24

Weight (kDa)

8.83

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 22, 484
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 498
AcsI RAATTY 1 cut(s) 393
AfaI GTAC 3 cut(s) 5, 16, 361
AgsI TTSAA 3 cut(s) 23, 298, 507
AluBI AGCT 5 cut(s) 167, 182, 239, 262, 313
AluI AGCT 5 cut(s) 167, 182, 239, 262, 313
Alw21I GWGCWC 1 cut(s) 264
AlwI GGATC 1 cut(s) 134
AoxI GGCC 2 cut(s) 148, 498
ApeKI GCWGC 1 cut(s) 236
ApoI RAATTY 1 cut(s) 393
ArsI GACNNNNNNTTYG 2 cut(s) 517, 549
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 545
AsuNHI GCTAGC 1 cut(s) 230
AvaII GGWCC 1 cut(s) 545
BanII GRGCYC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 248
BccI CCATC 1 cut(s) 488
BclI TGATCA 1 cut(s) 175
BfaI CTAG 4 cut(s) 45, 152, 231, 557
BfuAI ACCTGC 2 cut(s) 22, 484
BisI GCNGC 1 cut(s) 237
BlsI GCNGC 1 cut(s) 238
Bme18I GGWCC 1 cut(s) 545
BmgT120I GGNCC 1 cut(s) 545
BmsI GCATC 2 cut(s) 91, 285
BmtI GCTAGC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 99
Bpu10I CCTNAGC 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 31
BsaJI CCNNGG 3 cut(s) 76, 145, 529
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 1 cut(s) 116
BseDI CCNNGG 3 cut(s) 76, 145, 529
BseGI GGATG 1 cut(s) 106
BseMII CTCAG 2 cut(s) 74, 413
BseNI ACTGG 1 cut(s) 116
BseXI GCAGC 1 cut(s) 248
BshFI GGCC 2 cut(s) 150, 500
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 2 cut(s) 150, 500
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 2 cut(s) 126, 175
Bsp19I CCATGG 2 cut(s) 76, 529
BspANI GGCC 2 cut(s) 150, 500
BspCNI CTCAG 2 cut(s) 73, 412
BspMI ACCTGC 2 cut(s) 22, 484
BspOI GCTAGC 1 cut(s) 234
BspPI GGATC 1 cut(s) 134
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 3 cut(s) 76, 145, 529
BssMI GATC 2 cut(s) 126, 175
BssT1I CCWWGG 3 cut(s) 76, 145, 529
Bst4CI ACNGT 2 cut(s) 71, 541
BstC8I GCNNGC 4 cut(s) 83, 232, 350, 444
BstDEI CTNAG 2 cut(s) 60, 399
BstDSI CCRYGG 2 cut(s) 76, 529
BstF5I GGATG 1 cut(s) 106
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 2 cut(s) 129, 178
BstMBI GATC 2 cut(s) 126, 175
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNSI RCATGY 1 cut(s) 446
BstV1I GCAGC 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 123
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 2 cut(s) 150, 500
BtgI CCRYGG 2 cut(s) 76, 529
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 2 cut(s) 123, 407
BveI ACCTGC 2 cut(s) 22, 484
Cac8I GCNNGC 4 cut(s) 83, 232, 350, 444
CfoI GCGC 1 cut(s) 236
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 1 cut(s) 545
Csp6I GTAC 3 cut(s) 4, 15, 360
CviAII CATG 3 cut(s) 77, 443, 530
CviQI GTAC 3 cut(s) 4, 15, 360
DdeI CTNAG 2 cut(s) 60, 399
DpnI GATC 2 cut(s) 128, 177
DpnII GATC 2 cut(s) 126, 175
EaeI YGGCCR 1 cut(s) 498
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 3 cut(s) 76, 145, 529
Eco24I GRGCYC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 545
Eco53kI GAGCTC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 262
EcoT14I CCWWGG 3 cut(s) 76, 145, 529
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 3 cut(s) 76, 145, 529
FaeI CATG 3 cut(s) 80, 446, 533
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FaqI GGGAC 1 cut(s) 438
FatI CATG 3 cut(s) 76, 442, 529
FauNDI CATATG 1 cut(s) 407
FbaI TGATCA 1 cut(s) 175
Fnu4HI GCNGC 1 cut(s) 237
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 1 cut(s) 237
FspBI CTAG 4 cut(s) 45, 152, 231, 557
GlaI GCGC 1 cut(s) 235
GluI GCNGC 1 cut(s) 237
GsuI CTGGAG 1 cut(s) 99
HaeIII GGCC 2 cut(s) 150, 500
HapII CCGG 1 cut(s) 501
HhaI GCGC 1 cut(s) 236
Hin1II CATG 3 cut(s) 80, 446, 533
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 180
HpaII CCGG 1 cut(s) 501
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 1 cut(s) 537
Hpy188III TCNNGA 2 cut(s) 332, 507
Hpy8I GTNNAC 1 cut(s) 430
HpyCH4III ACNGT 2 cut(s) 71, 541
HpyCH4V TGCA 5 cut(s) 31, 104, 209, 252, 405
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 2 cut(s) 60, 399
Hsp92II CATG 3 cut(s) 80, 446, 533
HspAI GCGC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 2 cut(s) 126, 175
LmnI GCTCC 2 cut(s) 259, 416
LpnPI CCDG 7 cut(s) 17, 129, 153, 345, 381, 489, 514
Lsp1109I GCAGC 1 cut(s) 248
LweI GCATC 2 cut(s) 91, 285
MaeI CTAG 4 cut(s) 45, 152, 231, 557
MaeIII GTNAC 1 cut(s) 71
MalI GATC 2 cut(s) 128, 177
MboI GATC 2 cut(s) 126, 175
MboII GAAGA 1 cut(s) 310
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 4 cut(s) 18, 93, 384, 393
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 3 cut(s) 69, 196, 383
MseI TTAA 2 cut(s) 41, 512
MspA1I CMGCKG 2 cut(s) 167, 239
MspI CCGG 1 cut(s) 501
MwoI GCNNNNNNNGC 1 cut(s) 236
NcoI CCATGG 2 cut(s) 76, 529
NdeI CATATG 1 cut(s) 407
NdeII GATC 2 cut(s) 126, 175
NheI GCTAGC 1 cut(s) 230
NlaIII CATG 3 cut(s) 80, 446, 533
NmuCI GTSAC 1 cut(s) 71
NspI RCATGY 1 cut(s) 446
PaeI GCATGC 1 cut(s) 446
PkrI GCNGC 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 545
PsuI RGATCY 1 cut(s) 126
PvuII CAGCTG 2 cut(s) 167, 239
RsaI GTAC 3 cut(s) 5, 16, 361
RsaNI GTAC 3 cut(s) 4, 15, 360
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 2 cut(s) 41, 512
SatI GCNGC 1 cut(s) 237
Sau3AI GATC 2 cut(s) 126, 175
Sau96I GGNCC 1 cut(s) 545
SduI GDGCHC 1 cut(s) 264
SetI ASST 9 cut(s) 36, 147, 169, 184, 241, 264, 315, 478, 550
SfaNI GCATC 2 cut(s) 91, 285
SinI GGWCC 1 cut(s) 545
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
SphI GCATGC 1 cut(s) 446
Sse9I AATT 4 cut(s) 18, 93, 384, 393
SspMI CTAG 4 cut(s) 45, 152, 231, 557
SstI GAGCTC 1 cut(s) 264
StyI CCWWGG 3 cut(s) 76, 145, 529
TaaI ACNGT 2 cut(s) 71, 541
TaqI TCGA 1 cut(s) 523
TasI AATT 4 cut(s) 18, 93, 384, 393
TatI WGTACW 2 cut(s) 3, 14
Tru1I TTAA 2 cut(s) 41, 512
Tru9I TTAA 2 cut(s) 41, 512
TscAI CASTG 2 cut(s) 123, 407
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 1 cut(s) 297
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 123, 407
VpaK11BI GGWCC 1 cut(s) 545
XapI RAATTY 1 cut(s) 393
XceI RCATGY 1 cut(s) 446
XspI CTAG 4 cut(s) 45, 152, 231, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.