Prupe.4G127700_v2.0.a1

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000508
Physical Location & Seq
Forward (+)
7017754 .. 7021166
3413 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G127700.1

Sequence Viewer

Length: 531 bp
ATGAGGTTGCACAACAGCAATGTCTATGATGAGCCCATAAAGGGTACTTCAGAATCAGTAGAGTGGATATTTTTAGTCACTAGCCTTGGCCTAGAGATGTTGTCAGCTGCTTTTGATCAGGCTTCCTCCCCGACAAAACCGCACTATGCGCTATTTGGTGTGCTGTTGGCTACTGCAGCTTTACTTATTTCCATCTGGGAGCTAATTTATAATGGTAGAAAGGAAAGAGTTGTGCTAAGGAGATGGGGAAGGCTCTGGTGGTTTTATTATCCACCTCCTCGGAACGCATTTTTCAGTACTCTTCCTGACATTTATGGACTAGTTGGCGGCATCTCGCAGTGCATTTGCTCTGTAGTTCAGTACATGTACTACCTTCGGCATGCCGATAATCCTATCAAAGTATCCATTTTGCCTGCCATATTTCTTAGGTGCTTAGGTGGTTCAAAACTAAATAGGAATCAGCGGAACATGAACACCATCGAACATAAGATGTTTAGGAAAAGGCAACTCTTGCACAGTGAGTCTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

20.35

Weight (kDa)

9.6

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 210
AciI CCGC 3 cut(s) 140, 327, 463
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 4 cut(s) 46, 298, 362, 368
AfiI CCNNNNNNNGG 1 cut(s) 41
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 1 cut(s) 444
AhlI ACTAGT 1 cut(s) 319
AluBI AGCT 3 cut(s) 107, 179, 202
AluI AGCT 3 cut(s) 107, 179, 202
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 2 cut(s) 107, 176
AspLEI GCGC 1 cut(s) 151
BanII GRGCYC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 94, 188
BccI CCATC 3 cut(s) 200, 237, 485
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 1 cut(s) 115
BcuI ACTAGT 1 cut(s) 319
BfaI CTAG 3 cut(s) 81, 92, 320
BfmI CTRYAG 2 cut(s) 174, 351
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 3 cut(s) 108, 177, 328
BlsI GCNGC 3 cut(s) 109, 178, 329
BmcAI AGTACT 1 cut(s) 298
BmsI GCATC 1 cut(s) 339
Bpu10I CCTNAGC 2 cut(s) 236, 433
BsaJI CCNNGG 2 cut(s) 85, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse3DI GCAATG 1 cut(s) 25
BseDI CCNNGG 2 cut(s) 85, 278
BseLI CCNNNNNNNGG 1 cut(s) 41
BseMI GCAATG 1 cut(s) 25
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 2 cut(s) 94, 188
BshFI GGCC 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 41
BsnI GGCC 1 cut(s) 90
Bsp1286I GDGCHC 1 cut(s) 36
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 3 cut(s) 140, 327, 463
BspANI GGCC 1 cut(s) 90
BspMAI CTGCAG 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 25
BssECI CCNNGG 2 cut(s) 85, 278
BssMI GATC 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 85
Bst4CI ACNGT 1 cut(s) 518
Bst6I CTCTTC 1 cut(s) 306
BstAPI GCANNNNNTGC 1 cut(s) 511
BstC8I GCNNGC 2 cut(s) 381, 414
BstDEI CTNAG 3 cut(s) 236, 425, 433
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 1 cut(s) 118
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 3 cut(s) 148, 176, 511
BstNSI RCATGY 2 cut(s) 367, 383
BstSFI CTRYAG 2 cut(s) 174, 351
BstV1I GCAGC 2 cut(s) 94, 188
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 1 cut(s) 90
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 2 cut(s) 344, 523
Cac8I GCNNGC 2 cut(s) 381, 414
CfoI GCGC 1 cut(s) 151
Csp6I GTAC 4 cut(s) 45, 297, 361, 367
CviAII CATG 3 cut(s) 364, 380, 469
CviJI RGCY 9 cut(s) 34, 84, 90, 107, 122, 170, 179, 202, 253
CviKI_1 RGCY 9 cut(s) 34, 84, 90, 107, 122, 170, 179, 202, 253
CviQI GTAC 4 cut(s) 45, 297, 361, 367
DdeI CTNAG 3 cut(s) 236, 425, 433
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 3 cut(s) 367, 383, 472
FatI CATG 3 cut(s) 363, 379, 468
FbaI TGATCA 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 108, 177, 328
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 3 cut(s) 108, 177, 328
FspBI CTAG 3 cut(s) 81, 92, 320
GlaI GCGC 1 cut(s) 150
GluI GCNGC 3 cut(s) 108, 177, 328
HaeIII GGCC 1 cut(s) 90
HhaI GCGC 1 cut(s) 151
Hin1II CATG 3 cut(s) 367, 383, 472
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 3 cut(s) 53, 457, 521
Hpy188I TCNGA 2 cut(s) 52, 282
Hpy188III TCNNGA 1 cut(s) 305
HpyAV CCTTC 2 cut(s) 243, 383
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4V TGCA 4 cut(s) 10, 176, 342, 514
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 176, 511
HpyF3I CTNAG 3 cut(s) 236, 425, 433
Hsp92II CATG 3 cut(s) 367, 383, 472
HspAI GCGC 1 cut(s) 149
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 5 cut(s) 104, 181, 241, 318, 426
Lsp1109I GCAGC 2 cut(s) 94, 188
LweI GCATC 1 cut(s) 339
MaeI CTAG 3 cut(s) 81, 92, 320
MaeIII GTNAC 1 cut(s) 76
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 1 cut(s) 293
MhlI GDGCHC 1 cut(s) 36
MluCI AATT 1 cut(s) 204
MlyI GAGTC 1 cut(s) 530
MnlI CCTC 3 cut(s) 136, 285, 288
MspA1I CMGCKG 2 cut(s) 107, 463
MwoI GCNNNNNNNGC 3 cut(s) 148, 176, 511
NdeII GATC 1 cut(s) 115
NlaIII CATG 3 cut(s) 367, 383, 472
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 2 cut(s) 367, 383
PaeI GCATGC 1 cut(s) 383
PciI ACATGT 1 cut(s) 363
PfeI GAWTC 2 cut(s) 53, 457
PkrI GCNGC 3 cut(s) 109, 178, 329
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
PscI ACATGT 1 cut(s) 363
PsiI TTATAA 1 cut(s) 210
PstI CTGCAG 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 4 cut(s) 46, 298, 362, 368
RsaNI GTAC 4 cut(s) 45, 297, 361, 367
SatI GCNGC 3 cut(s) 108, 177, 328
Sau3AI GATC 1 cut(s) 115
ScaI AGTACT 1 cut(s) 298
SchI GAGTC 1 cut(s) 530
SduI GDGCHC 1 cut(s) 36
SetI ASST 8 cut(s) 8, 109, 181, 204, 277, 375, 431, 439
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 2 cut(s) 174, 351
SpeI ACTAGT 1 cut(s) 319
SphI GCATGC 1 cut(s) 383
Sse9I AATT 1 cut(s) 204
SsiI CCGC 3 cut(s) 140, 327, 463
SspMI CTAG 3 cut(s) 81, 92, 320
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 1 cut(s) 518
TaqI TCGA 1 cut(s) 480
TasI AATT 1 cut(s) 204
TatI WGTACW 3 cut(s) 296, 360, 366
TauI GCSGC 1 cut(s) 330
TfiI GAWTC 2 cut(s) 53, 457
TscAI CASTG 2 cut(s) 344, 523
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 2 cut(s) 107, 176
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 1 cut(s) 485
TspRI CASTG 2 cut(s) 344, 523
XceI RCATGY 2 cut(s) 367, 383
XspI CTAG 3 cut(s) 81, 92, 320
ZrmI AGTACT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.