Rorug05G0582500

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000144
Physical Location & Seq
Forward (+)
77950808 .. 77955670
4863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0582500.1

Sequence Viewer

Length: 3033 bp
ATGGCCGAGATAGCATCCCTTGTGCTTTCCACTGGCATTACGTCGGCAATTCTGCAGGTTGTCGTCGAGAGAGTCGCAAAGTTTGTTGAACAAAAGAAACACTTGTTTTCTAGGGTGGACGACAATCTCAGAAAGTTGAATAGAACGCTGATGAACATTCAAGCTCGTGTTGATGATCTAGAAAAGAAACATTGCATCACGCAGGCAGCAGAAGATTTGCTCGAAGATCTCAAGACTAGTTTTTTGGATGCTGAAGATTTGCTGGACAAGATTGAAGTGGAGGTCAACAGTTGGAATCAGGTACATCACTTGATCCCCTTCTATTCTAGCATACCGTCTGAGATTAAAAAGATGCAAGTGAAATTGGATGAGTTAGTAACAGAACTGGAAAGTTACTCTACCACAGAAATCATCCAGTCAAGAGGGCCTAAAGAAAAGACTATTCCTAATTCGACTTCATTGGTTGATAAATCATGTGTGTTTGGGAGGGATGAAGACATGTACAAGATTAAGAAATTGACATTTTCTGTACAAGGAGAAGAGAGAAATTTCTCTGTAATTTCAATAGTGGGCATGGGTGGGATTGGGAAAACAACACTTGCTCAACTCTTCTACAATGATGCAGATGTGCATAAAAAGTTTGATTTGAAATTATGGGTGTCTGTTTCTTTAGACTATGGTCTAGACATGATTACAAAATCTATTCTTGAGTCTGCCACTGGAGGAAAGGGTTCTCAGTTGTCAAGTTCGGATAGTGTTCAGGTTGAATTACAGAAGACATTGAAGGGGAAGAGGTTTTTACTTGTACTAGATGGCATTTGTAATGATAATCTCAGTGATTGGGATCGACTCAGGTTGTCTTTTATGGATGTAAATAAGGGGAGCAAGATTGTGATGACTACTCGAAGTGAAATTTCTGCCTCAATTGTTGCCTCTACATCATATCGTTTGGATCCTCTGTGTGACAATGCTTGTTGGGAAATAATCAAACAAAGAGGGAATCTTAATGGGCAGACTAATTTGGAGGCGAATGGCCTAGCAATTTCGAAGAAGTGCAAAGGATTTCCATGGGTAGCAAAAGTGATTGGAAGCTGTTTGAATTCGGGAGAGATGGAGTGGGATGCTTTGTTAAAGTACGGGCTTTGGGAACTGCAGGAACATAAAACACGAATCTATCCAGTCCTCAAATTGTTGAGTTATGATAATATGCCTTCTTATTTGAAAAGATGTTTCAGTTATTGTTCTATCTTTCCTCGTGATCATGTAGTTGAGAAGGATGATTTGGTCCAACTATGGGCAGCAGAAGGCTTTATTCAGTCCCAAGGAACAAGAGAAATTGAAGATGTTGGAAGACAGTATTTTGAGGACTTGCGTTCGAGGGCTTTCTTTTCACAAGTTCCACATATTGGTGGTGATCAGCAGAAATATAAAATGCATGAGCTCATTCATGATTTGGCAAGATTTGTTTCAACCAACTTATGCTTTTGTATGGAGGACAATATGCCATGCTTGGTGCACATATCTCCTAATGCTCGGTATGCGTCCTTGCTATATGAAAATACTCAACTATCTACATTGGAGGCGTTTTACAAGTATGAGAAGTTGAGGACATTTACATTGCTTCCTAAATTCCCATCTATTCCAAGGCCAGTCATGTTGCTTCCCAATTTTTCGTCCAATCTTGGCGAAGTCCCTCATGAATTTTTTGAAAGGTTGGGATGCTTGAGGGTGTTGAATTTGAGTCATAGTGCCATCTCTAAATTGCCTGAATCTATTGGAAATTTGATTCATCTTCGTTACCTCAATCTTAGTCATACCTGTATTATACTATTGCCAAAGCCTATAACAACGATATATGGGTTAGAAACATTGAAGCTCAAGAGTTGTCCTAAACTTCTTCATTTGCCTGAGAACTTAAAGGACTTGATTAAGCTAAGACATCTTGACTTTGACAGACACCGTCAACTAAGTTCCATGCCAATAGATGTTGGGAAGTTAACTAGTCTTGAAACCCTTCATGCATTTAAAGTGGGAAAAGAGAAAGGATATCAGATTGAGGAGCTTAAGAACATGAGGTGTCTTCGTGGATCCATTTCTATTACAAATCTTGAGAATGTGGCAGATTCTATGCAGGCTGAGGCTGCTATGTTACACAACAAGCAATACTTAGACAGGTTGGAGCTAGAATGGAATGGAATCAGAGGTCAAATGCAACCAGTTCAACAAGAAGTTCTTACTGGGCTTGAACCACATGCTGGATTAAAGGAGTTACGAGTAACAGGTTACTGTGGTTTGTTATTTCCCAGATGGATAGCCAACTCAGAATTTAGCAAGCTTGAGAGTATTGAATTAGAAAATTGCCCATTTTGTGTACTTCTTCCTTCTCTTGGCCAACTTCCGGCTCTCAAGAAACTTCTTATTCAAAATATGTCTACTTTGGAAGGTGTGGATCGTCTGTTTTGTGGTTCCTCAACTGCTTTTCTATCCTTGGAGACGCTAACACTTCGTGACATGCCGGAGTTGAGTAACTGGTCAGGGCTACGTGACAATGACATGCCTTACCTCAGAGACCTCTTCATTGATTCTTGTCCAAAGTTGGCCACCCTACCTTCATTTCATTACCTCACTTCACTTCAATGTTTAGACATCAATCGTTGCCCTCAGCTTGAATCTTTGCCAGAGGGAGAACTGCCACCCTCACTCAAAACTTTGATCATTTTGGATAGTGACATCTTGAAGGATCGGTGCAAGGTGGGAGAAGGTGCAGATTGGAATAAGATCAGATGGATCCCTACCATATTGATTGAAAATATGGTGATTCCTGCACAAAGACCAGATAACCAGATTGATGAGGTTCAGAGTGCTCCAGAAAACATGTTTGGTGGCAGAAACCCCTTGTCGATTTGTTCTGTTATTCCAGAAAACATGTTCGGTAGCAGAAACCCCTTGTCGATTTGTTCTGTTATTCTTTTTCCTCTGCTCATCCTTTTTATGAGATCCTTGTACCGGTACTTGTCCCAAGCCAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

1010

Amino Acids

114.73

Weight (kDa)

6.18

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 18 - 100 2.4e-11 Rx N-terminal domain
NB-ARC PF00931 180 - 333 1.4e-29 NB-ARC domain
WHD_DRP PF23559 416 - 486 9.1e-25 Disease resistance protein Winged helix domain
LRR_8 PF13855 558 - 606 2.6e-07 Leucine rich repeat
LRR_14 PF23598 571 - 859 5.3e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 685 - 810 4.4e-37 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 46
AccB7I CCANNNNNTGG 2 cut(s) 1406, 2255
AccI GTMKAC 1 cut(s) 2432
AcoI YGGCCR 3 cut(s) 3, 2389, 2598
AcsI RAATTY 8 cut(s) 547, 912, 1099, 1628, 1700, 1735, 1780, 2324
AcuI CTGAAG 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 2507
AfaI GTAC 8 cut(s) 303, 503, 531, 807, 1136, 2373, 3005, 3011
AfiI CCNNNNNNNGG 6 cut(s) 1294, 1406, 2255, 2387, 2398, 3006
AflII CTTAAG 1 cut(s) 2063
AflIII ACRYGT 3 cut(s) 498, 2874, 2925
AgeI ACCGGT 1 cut(s) 3006
AhlI ACTAGT 2 cut(s) 236, 2000
AjuI GAANNNNNNNTTGG 4 cut(s) 1265, 1297, 2862, 2894
AluBI AGCT 9 cut(s) 164, 1092, 1441, 1876, 1933, 2062, 2182, 2335, 2665
AluI AGCT 9 cut(s) 164, 1092, 1441, 1876, 1933, 2062, 2182, 2335, 2665
Alw21I GWGCWC 3 cut(s) 1443, 1518, 2866
Alw26I GTCTC 2 cut(s) 2486, 2562
Alw44I GTGCAC 1 cut(s) 1514
AoxI GGCC 6 cut(s) 3, 425, 1033, 1646, 2389, 2598
ApaLI GTGCAC 1 cut(s) 1514
ApeKI GCWGC 3 cut(s) 206, 1298, 2141
ApoI RAATTY 8 cut(s) 547, 912, 1099, 1628, 1700, 1735, 1780, 2324
ArsI GACNNNNNNTTYG 2 cut(s) 226, 258
AsiGI ACCGGT 1 cut(s) 3006
Asp700I GAANNNNTTC 2 cut(s) 730, 2013
AspS9I GGNCC 2 cut(s) 425, 1285
AsuHPI GGTGA 2 cut(s) 1424, 2827
AsuII TTCGAA 1 cut(s) 1046
AvaII GGWCC 1 cut(s) 1285
BaeGI GKGCMC 1 cut(s) 1518
BalI TGGCCA 2 cut(s) 2391, 2600
BamHI GGATCC 3 cut(s) 952, 2087, 2787
BanII GRGCYC 1 cut(s) 1443
BauI CACGAG 2 cut(s) 165, 1254
BbsI GAAGAC 4 cut(s) 501, 782, 1357, 2072
Bbv12I GWGCWC 3 cut(s) 1443, 1518, 2866
BbvCI CCTCAGC 2 cut(s) 2136, 2661
BbvI GCAGC 3 cut(s) 218, 1310, 2128
BccI CCATC 6 cut(s) 806, 1105, 1642, 1760, 2301, 2778
BclI TGATCA 3 cut(s) 1258, 1414, 2712
BcoDI GTCTC 2 cut(s) 2486, 2562
BcuI ACTAGT 2 cut(s) 236, 2000
BfaI CTAG 9 cut(s) 111, 179, 237, 327, 683, 809, 1037, 2001, 2183
BfmI CTRYAG 2 cut(s) 53, 1151
BfrI CTTAAG 1 cut(s) 2063
BfuAI ACCTGC 1 cut(s) 46
BglII AGATCT 1 cut(s) 226
BisI GCNGC 3 cut(s) 207, 1299, 2142
BlsI GCNGC 3 cut(s) 208, 1300, 2143
Bme18I GGWCC 1 cut(s) 1285
BmgT120I GGNCC 2 cut(s) 425, 1285
BmiI GGNNCC 4 cut(s) 954, 2089, 2467, 2789
BmrI ACTGGG 1 cut(s) 2247
BmsI GCATC 7 cut(s) 23, 204, 238, 342, 610, 1111, 1709
BmuI ACTGGG 1 cut(s) 2247
BoxI GACNNNNGTC 1 cut(s) 678
BpiI GAAGAC 4 cut(s) 501, 782, 1357, 2072
BpmI CTGGAG 2 cut(s) 741, 2850
Bpu10I CCTNAGC 2 cut(s) 2136, 2661
Bpu14I TTCGAA 1 cut(s) 1046
BpuEI CTTGAG 7 cut(s) 215, 728, 1744, 1862, 2129, 2357, 2390
BsaAI YACGTR 1 cut(s) 2543
BsaBI GATNNNNATC 1 cut(s) 843
BsaI GGTCTC 1 cut(s) 2562
BsaJI CCNNGG 4 cut(s) 1067, 1321, 1643, 2487
BsaWI WCCGGW 1 cut(s) 3006
Bsc4I CCNNNNNNNGG 6 cut(s) 1294, 1406, 2255, 2387, 2398, 3006
Bse118I RCCGGY 1 cut(s) 3006
Bse1I ACTGG 9 cut(s) 37, 390, 415, 724, 1178, 1649, 2216, 2242, 2534
Bse3DI GCAATG 2 cut(s) 190, 1616
Bse8I GATNNNNATC 1 cut(s) 843
BseDI CCNNGG 4 cut(s) 1067, 1321, 1643, 2487
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 6 cut(s) 1294, 1406, 2255, 2387, 2398, 3006
BseMI GCAATG 2 cut(s) 190, 1616
BseNI ACTGG 9 cut(s) 37, 390, 415, 724, 1178, 1649, 2216, 2242, 2534
BseRI GAGGAG 1 cut(s) 2072
BseSI GKGCMC 1 cut(s) 1518
BseXI GCAGC 3 cut(s) 218, 1310, 2128
BsgI GTGCAG 2 cut(s) 2784, 2808
BshFI GGCC 6 cut(s) 5, 427, 1035, 1648, 2391, 2600
BshTI ACCGGT 1 cut(s) 3006
BsiHKAI GWGCWC 3 cut(s) 1443, 1518, 2866
BsiSI CCGG 3 cut(s) 2399, 2516, 3007
BslFI GGGAC 3 cut(s) 1303, 1676, 3001
BslI CCNNNNNNNGG 6 cut(s) 1294, 1406, 2255, 2387, 2398, 3006
BsmAI GTCTC 2 cut(s) 2486, 2562
BsmBI CGTCTC 1 cut(s) 2486
BsmFI GGGAC 3 cut(s) 1303, 1676, 3001
BsnI GGCC 6 cut(s) 5, 427, 1035, 1648, 2391, 2600
Bso31I GGTCTC 1 cut(s) 2562
Bsp119I TTCGAA 1 cut(s) 1046
Bsp1286I GDGCHC 3 cut(s) 1443, 1518, 2866
Bsp1407I TGTACA 2 cut(s) 501, 529
Bsp19I CCATGG 1 cut(s) 1067
BspANI GGCC 6 cut(s) 5, 427, 1035, 1648, 2391, 2600
BspHI TCATGA 2 cut(s) 1447, 1696
BspLI GGNNCC 4 cut(s) 954, 2089, 2467, 2789
BspMAI CTGCAG 2 cut(s) 57, 1155
BspMI ACCTGC 1 cut(s) 46
BspT104I TTCGAA 1 cut(s) 1046
BspTI CTTAAG 1 cut(s) 2063
BspTNI GGTCTC 1 cut(s) 2562
BsrDI GCAATG 2 cut(s) 190, 1616
BsrFI RCCGGY 1 cut(s) 3006
BsrGI TGTACA 2 cut(s) 501, 529
BsrI ACTGG 9 cut(s) 37, 390, 415, 724, 1178, 1649, 2216, 2242, 2534
BssAI RCCGGY 1 cut(s) 3006
BssECI CCNNGG 4 cut(s) 1067, 1321, 1643, 2487
BssSI CACGAG 2 cut(s) 165, 1254
BssT1I CCWWGG 4 cut(s) 1067, 1321, 1643, 2487
Bst2BI CACGAG 2 cut(s) 165, 1254
Bst4CI ACNGT 5 cut(s) 290, 336, 1356, 1961, 2288
Bst6I CTCTTC 4 cut(s) 534, 614, 785, 2579
BstAFI CTTAAG 1 cut(s) 2063
BstAUI TGTACA 2 cut(s) 501, 529
BstBAI YACGTR 1 cut(s) 2543
BstBI TTCGAA 1 cut(s) 1046
BstC8I GCNNGC 3 cut(s) 204, 2133, 2333
BstDSI CCRYGG 1 cut(s) 1067
BstMAI GTCTC 2 cut(s) 2486, 2562
BstMWI GCNNNNNNNGC 3 cut(s) 11, 1538, 2141
BstNSI RCATGY 6 cut(s) 502, 2255, 2515, 2557, 2878, 2929
BstPAI GACNNNNGTC 1 cut(s) 678
BstSFI CTRYAG 2 cut(s) 53, 1151
BstSLI GKGCMC 1 cut(s) 1518
BstV1I GCAGC 3 cut(s) 218, 1310, 2128
BstV2I GAAGAC 4 cut(s) 501, 782, 1357, 2072
BstX2I RGATCY 5 cut(s) 226, 952, 2087, 2787, 2996
BstYI RGATCY 5 cut(s) 226, 952, 2087, 2787, 2996
BsuRI GGCC 6 cut(s) 5, 427, 1035, 1648, 2391, 2600
BtgI CCRYGG 1 cut(s) 1067
BtsIMutI CAGTG 3 cut(s) 30, 717, 841
BveI ACCTGC 1 cut(s) 46
Cac8I GCNNGC 3 cut(s) 204, 2133, 2333
CciI TCATGA 2 cut(s) 1447, 1696
Cfr10I RCCGGY 1 cut(s) 3006
Cfr13I GGNCC 2 cut(s) 425, 1285
CseI GACGC 2 cut(s) 1530, 2503
Csp6I GTAC 8 cut(s) 302, 502, 530, 806, 1135, 2372, 3004, 3010
CspAI ACCGGT 1 cut(s) 3006
CviQI GTAC 8 cut(s) 302, 502, 530, 806, 1135, 2372, 3004, 3010
DraI TTTAAA 1 cut(s) 2026
DraIII CACNNNGTG 1 cut(s) 2507
EaeI YGGCCR 3 cut(s) 3, 2389, 2598
Eam1104I CTCTTC 4 cut(s) 534, 614, 785, 2579
EarI CTCTTC 4 cut(s) 534, 614, 785, 2579
Ecl136II GAGCTC 1 cut(s) 1441
Eco130I CCWWGG 4 cut(s) 1067, 1321, 1643, 2487
Eco24I GRGCYC 1 cut(s) 1443
Eco31I GGTCTC 1 cut(s) 2562
Eco32I GATATC 1 cut(s) 2048
Eco47I GGWCC 1 cut(s) 1285
Eco53kI GAGCTC 1 cut(s) 1441
Eco57I CTGAAG 1 cut(s) 273
EcoICRI GAGCTC 1 cut(s) 1441
EcoO109I RGGNCCY 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 1099
EcoRV GATATC 1 cut(s) 2048
EcoT14I CCWWGG 4 cut(s) 1067, 1321, 1643, 2487
EcoT22I ATGCAT 2 cut(s) 1437, 2023
EcoT38I GRGCYC 1 cut(s) 1443
ErhI CCWWGG 4 cut(s) 1067, 1321, 1643, 2487
Esp3I CGTCTC 1 cut(s) 2486
FalI AAGNNNNNCTT 6 cut(s) 86, 118, 2150, 2182, 2217, 2249
FaqI GGGAC 3 cut(s) 1303, 1676, 3001
FbaI TGATCA 3 cut(s) 1258, 1414, 2712
FblI GTMKAC 1 cut(s) 2432
Fnu4HI GCNGC 3 cut(s) 207, 1299, 2142
FokI GGATG 9 cut(s) 260, 380, 398, 503, 881, 1133, 1289, 1731, 2969
FriOI GRGCYC 1 cut(s) 1443
Fsp4HI GCNGC 3 cut(s) 207, 1299, 2142
FspBI CTAG 9 cut(s) 111, 179, 237, 327, 683, 809, 1037, 2001, 2183
GluI GCNGC 3 cut(s) 207, 1299, 2142
GsuI CTGGAG 2 cut(s) 741, 2850
HaeIII GGCC 6 cut(s) 5, 427, 1035, 1648, 2391, 2600
HapII CCGG 3 cut(s) 2399, 2516, 3007
HgaI GACGC 2 cut(s) 1530, 2503
HincII GTYRAC 3 cut(s) 286, 1964, 1998
HindII GTYRAC 3 cut(s) 286, 1964, 1998
HindIII AAGCTT 1 cut(s) 2333
HpaI GTTAAC 1 cut(s) 1998
HpaII CCGG 3 cut(s) 2399, 2516, 3007
HphI GGTGA 2 cut(s) 1424, 2827
Hpy166II GTNNAC 7 cut(s) 118, 286, 1516, 1964, 1998, 2372, 2433
Hpy188I TCNGA 9 cut(s) 131, 340, 751, 2052, 2201, 2323, 2567, 2783, 2859
Hpy8I GTNNAC 7 cut(s) 118, 286, 1516, 1964, 1998, 2372, 2433
Hpy99I CGWCG 2 cut(s) 46, 68
HpyCH4III ACNGT 5 cut(s) 290, 336, 1356, 1961, 2288
HpyCH4IV ACGT 2 cut(s) 41, 2542
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 1538, 2141
HpySE526I ACGT 2 cut(s) 41, 2542
Ksp22I TGATCA 3 cut(s) 1258, 1414, 2712
KspAI GTTAAC 1 cut(s) 1998
LmnI GCTCC 4 cut(s) 882, 2059, 2179, 2869
Lsp1109I GCAGC 3 cut(s) 218, 1310, 2128
LweI GCATC 7 cut(s) 23, 204, 238, 342, 610, 1111, 1709
MaeI CTAG 9 cut(s) 111, 179, 237, 327, 683, 809, 1037, 2001, 2183
MaeII ACGT 2 cut(s) 41, 2542
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 5 cut(s) 226, 952, 2087, 2787, 2996
MhlI GDGCHC 3 cut(s) 1443, 1518, 2866
MlsI TGGCCA 2 cut(s) 2391, 2600
MluNI TGGCCA 2 cut(s) 2391, 2600
MlyI GAGTC 4 cut(s) 81, 719, 843, 1750
MmeI TCCRAC 4 cut(s) 272, 1312, 1327, 2157
Mox20I TGGCCA 2 cut(s) 2391, 2600
Mph1103I ATGCAT 2 cut(s) 1437, 2023
MroXI GAANNNNTTC 2 cut(s) 730, 2013
MscI TGGCCA 2 cut(s) 2391, 2600
MslI CAYNNNNRTG 2 cut(s) 1407, 2635
Msp20I TGGCCA 2 cut(s) 2391, 2600
MspCI CTTAAG 1 cut(s) 2063
MspI CCGG 3 cut(s) 2399, 2516, 3007
MunI CAATTG 1 cut(s) 924
MwoI GCNNNNNNNGC 3 cut(s) 11, 1538, 2141
NcoI CCATGG 1 cut(s) 1067
NlaIV GGNNCC 4 cut(s) 954, 2089, 2467, 2789
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 4 cut(s) 962, 2507, 2543, 2726
NsiI ATGCAT 2 cut(s) 1437, 2023
NspI RCATGY 6 cut(s) 502, 2255, 2515, 2557, 2878, 2929
NspV TTCGAA 1 cut(s) 1046
PagI TCATGA 2 cut(s) 1447, 1696
PciI ACATGT 3 cut(s) 498, 2874, 2925
PdmI GAANNNNTTC 2 cut(s) 730, 2013
PflFI GACNNNGTC 1 cut(s) 1959
PflMI CCANNNNNTGG 2 cut(s) 1406, 2255
PinAI ACCGGT 1 cut(s) 3006
PkrI GCNGC 3 cut(s) 208, 1300, 2143
PleI GAGTC 4 cut(s) 80, 718, 843, 1749
PpsI GAGTC 4 cut(s) 80, 718, 843, 1749
Ppu21I YACGTR 1 cut(s) 2543
PscI ACATGT 3 cut(s) 498, 2874, 2925
PshAI GACNNNNGTC 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 1443
PspN4I GGNNCC 4 cut(s) 954, 2089, 2467, 2789
PspPI GGNCC 2 cut(s) 425, 1285
PstI CTGCAG 2 cut(s) 57, 1155
PsuI RGATCY 5 cut(s) 226, 952, 2087, 2787, 2996
PsyI GACNNNGTC 1 cut(s) 1959
RsaI GTAC 8 cut(s) 303, 503, 531, 807, 1136, 2373, 3005, 3011
RsaNI GTAC 8 cut(s) 302, 502, 530, 806, 1135, 2372, 3004, 3010
RseI CAYNNNNRTG 2 cut(s) 1407, 2635
SacI GAGCTC 1 cut(s) 1443
SatI GCNGC 3 cut(s) 207, 1299, 2142
Sau96I GGNCC 2 cut(s) 425, 1285
SchI GAGTC 4 cut(s) 81, 719, 843, 1750
SduI GDGCHC 3 cut(s) 1443, 1518, 2866
SfaNI GCATC 7 cut(s) 23, 204, 238, 342, 610, 1111, 1709
SfcI CTRYAG 2 cut(s) 53, 1151
SfuI TTCGAA 1 cut(s) 1046
SinI GGWCC 1 cut(s) 1285
SmiMI CAYNNNNRTG 2 cut(s) 1407, 2635
SmlI CTYRAG 8 cut(s) 230, 707, 1723, 1877, 2063, 2108, 2336, 2405
SmoI CTYRAG 8 cut(s) 230, 707, 1723, 1877, 2063, 2108, 2336, 2405
SpeI ACTAGT 2 cut(s) 236, 2000
SspMI CTAG 9 cut(s) 111, 179, 237, 327, 683, 809, 1037, 2001, 2183
SstI GAGCTC 1 cut(s) 1443
StyI CCWWGG 4 cut(s) 1067, 1321, 1643, 2487
TaaI ACNGT 5 cut(s) 290, 336, 1356, 1961, 2288
TaiI ACGT 2 cut(s) 44, 2545
TaqI TCGA 9 cut(s) 66, 222, 452, 847, 904, 1046, 1376, 2900, 2951
TatI WGTACW 4 cut(s) 501, 529, 805, 2371
TscAI CASTG 3 cut(s) 37, 724, 841
TseFI GTSAC 4 cut(s) 962, 2507, 2543, 2726
TseI GCWGC 3 cut(s) 206, 1298, 2141
Tsp45I GTSAC 4 cut(s) 962, 2507, 2543, 2726
TspRI CASTG 3 cut(s) 37, 724, 841
Tth111I GACNNNGTC 1 cut(s) 1959
Van91I CCANNNNNTGG 2 cut(s) 1406, 2255
Vha464I CTTAAG 1 cut(s) 2063
VneI GTGCAC 1 cut(s) 1514
VpaK11BI GGWCC 1 cut(s) 1285
XapI RAATTY 8 cut(s) 547, 912, 1099, 1628, 1700, 1735, 1780, 2324
XbaI TCTAGA 2 cut(s) 178, 682
XceI RCATGY 6 cut(s) 502, 2255, 2515, 2557, 2878, 2929
XmiI GTMKAC 1 cut(s) 2432
XmnI GAANNNNTTC 2 cut(s) 730, 2013
XspI CTAG 9 cut(s) 111, 179, 237, 327, 683, 809, 1037, 2001, 2183
Zsp2I ATGCAT 2 cut(s) 1437, 2023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.