Rroxscaffold_7G00211600

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000144
Physical Location & Seq
Forward (+)
62069348 .. 62071859
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00211600.1

Sequence Viewer

Length: 1614 bp
ATGTTGCGGCAAAGGAAGAATCTGCTGAAGAAAAGGAAGGATATGGCGATGATTGAGAGAAAGACAAAGAAAAAAGAAAAGGAAAAGCAAAATCAAAATATCAGCAATGAGTGTAGCCCTTGGCAGACACCACCAGAAGATATCATGGAGAAGATTCTACAGTTTATCGACTTTGCTGATCAAAGAAGGTTAGGTCGGGTCAGCAAGTCTTGGAGGTCAATTGTTTTTCAAACATTCATGCGCAGTTCTCGTCGTCAACTCCCATGGTTAATACTCCCTGAAGCCCCAAATCGCAGGATCAACAAGCACTTGAGCTTTCTCACCTTCCCTGAGAATACACTCTCTAAGGGCAAGGCTGTCAATTTAAAGCTGCCTAAGCAACTACAAGGAGGGTGGATATACGGGTCTTCTAAAGGTTGGTTGATCATCATAAAGGAAAAGGGACTAAACTCTGAGATGTGTCTACTAAACCCAATTTCAGGAGTCCTACACAAACTTCCGCCCTTGAGAACAATTCCATCATTCAAAAACTTCGTAAAAACCAGGGAATGGGAACTTCTGGGTGCCGATGGATTCTGTAATCGCGTTAAAATGTCTACACGGGATGGTCATAATTCTACAAGCAATTTTACTGTAGCTGCAATTTTCGATGATCGGAATGCTTTATGTTTGTGCAGACCCGGAGACGAGAAATGGAGTGTCTTCCCGGTATTAGACTACCTCATTGATATATTGTTTTCTTCTGGCACGTTATATGCTTTAGTTTTGAGTGAACAAAATGATGGCGGCGTCGCACCTACTTGCCCCTTAAACTTTGCATATCATGATGCTGTACACTTGAAGTTGAAGTTGGTCTATGACAAACACGAAAGCCAGAATAACGTCATTGATGAATATCATAGTAACTATAAGATAGTTTCCAATGCATCATACGCACCAATGTTGTTAGAATCAACCAGCAAGGAAGTTCTGTTAATCCATCAAATGTTTGATTATGTTTTGAAGACAACAAATGATGGTGATGAACAAATCAATGAGAATGATGGCGGTCAAGGCCTAAATAATGATGGTGATGAGGAAGACATTGATGGTGTTGAAGGGAATAATGACGATGAAGGTGGTGGTGATGACAGTACTGATGATGAAGAAGTCGTCGATGAGAATACTAGAAGCATACGTCATCGCACAAGAAGTATTAGAACATACAAGATTGATCCATACAACAAGAATTTTCATATGATGCAATCCTTGGGTGACCAATTGTTATTTTGTGGAAACGATGATGCTTTCTCCCTTCCTGCTAGTAACATCAAACAAATAGACAATAATTGCATCTACTTTGCAGTGAATATGTACACAAAAAACGTGGACTTGGAATGGATTGCAAAGACGTATATGTCTAATGAGTTTGGCATATTCTACTTAGATGGTCAAAGAACTAAGAGGCCATTTCAAGGTATGGAAATGTCAGTGAAACTAGTTTTCTTGGAGATTCATCTGGAAGCTCTGTTTGCCTCCCACGCTAAACACCTTTATCCGGGTCATGAGTCATGTCAAACTACTTGCCGAGTTCGAGAGCTCGGAGTCATTTTACTAATGATGACTCTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

537

Amino Acids

61.62

Weight (kDa)

6.42

Isoelectric Point (pI)

47.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 45 - 75 7.1e-07 F-box domain
Beta-prop_KIB1-4 PF03478 114 - 466 1.5e-31 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1396
Acc16I TGCGCA 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 563
AccB7I CCANNNNNTGG 1 cut(s) 549
AccI GTMKAC 2 cut(s) 463, 596
AccII CGCG 1 cut(s) 585
AciI CCGC 4 cut(s) 7, 500, 786, 1047
AclWI GGATC 2 cut(s) 305, 1208
AcsI RAATTY 1 cut(s) 1228
AcuI CTGAAG 2 cut(s) 47, 300
AcyI GRCGYC 1 cut(s) 789
AfaI GTAC 3 cut(s) 834, 1135, 1355
AfiI CCNNNNNNNGG 4 cut(s) 479, 549, 1454, 1537
AgsI TTSAA 7 cut(s) 230, 526, 841, 847, 1003, 1097, 1454
AhlI ACTAGT 1 cut(s) 1477
AjnI CCWGG 1 cut(s) 542
AjuI GAANNNNNNNTTGG 2 cut(s) 833, 865
AluBI AGCT 5 cut(s) 315, 370, 638, 1505, 1579
AluI AGCT 5 cut(s) 315, 370, 638, 1505, 1579
Alw21I GWGCWC 1 cut(s) 1581
Alw26I GTCTC 1 cut(s) 678
AlwI GGATC 2 cut(s) 305, 1208
AoxI GGCC 2 cut(s) 1054, 1445
ApeKI GCWGC 2 cut(s) 370, 638
ApoI RAATTY 1 cut(s) 1228
AspLEI GCGC 1 cut(s) 243
AsuC2I CCSGG 3 cut(s) 681, 707, 1539
AsuHPI GGTGA 5 cut(s) 313, 1031, 1082, 1136, 1265
BanI GGYRCC 1 cut(s) 563
BanII GRGCYC 1 cut(s) 1581
BbsI GAAGAC 4 cut(s) 399, 694, 1010, 1086
Bbv12I GWGCWC 1 cut(s) 1581
BbvI GCAGC 2 cut(s) 357, 625
BciT130I CCWGG 1 cut(s) 544
BclI TGATCA 2 cut(s) 178, 423
BcnI CCSGG 3 cut(s) 681, 707, 1539
BcoDI GTCTC 1 cut(s) 678
BcuI ACTAGT 1 cut(s) 1477
BfaI CTAG 4 cut(s) 1167, 1302, 1478, 1612
BfmI CTRYAG 2 cut(s) 158, 633
BisI GCNGC 4 cut(s) 8, 371, 639, 787
BlsI GCNGC 4 cut(s) 9, 372, 640, 788
BmcAI AGTACT 1 cut(s) 1135
Bme1390I CCNGG 4 cut(s) 544, 681, 707, 1539
BmiI GGNNCC 1 cut(s) 565
BmrFI CCNGG 4 cut(s) 544, 681, 707, 1539
BmsI GCATC 5 cut(s) 817, 935, 1230, 1273, 1341
BoxI GACNNNNGTC 1 cut(s) 1607
BpiI GAAGAC 4 cut(s) 399, 694, 1010, 1086
Bpu10I CCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 331, 526
BpuMI CCSGG 3 cut(s) 681, 707, 1539
BsaBI GATNNNNATC 1 cut(s) 894
BsaHI GRCGYC 1 cut(s) 789
BsaJI CCNNGG 4 cut(s) 119, 263, 543, 1248
Bsc4I CCNNNNNNNGG 4 cut(s) 479, 549, 1454, 1537
Bse3DI GCAATG 1 cut(s) 112
Bse8I GATNNNNATC 1 cut(s) 894
BseBI CCWGG 1 cut(s) 544
BseDI CCNNGG 4 cut(s) 119, 263, 543, 1248
BseGI GGATG 1 cut(s) 610
BseJI GATNNNNATC 1 cut(s) 894
BseLI CCNNNNNNNGG 4 cut(s) 479, 549, 1454, 1537
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 2 cut(s) 321, 444
BseXI GCAGC 2 cut(s) 357, 625
BsgI GTGCAG 1 cut(s) 694
Bsh1236I CGCG 1 cut(s) 585
BshFI GGCC 2 cut(s) 1056, 1447
BshNI GGYRCC 1 cut(s) 563
BsiHKAI GWGCWC 1 cut(s) 1581
BsiSI CCGG 3 cut(s) 681, 707, 1538
BslFI GGGAC 1 cut(s) 456
BslI CCNNNNNNNGG 4 cut(s) 479, 549, 1454, 1537
BsmAI GTCTC 1 cut(s) 678
BsmBI CGTCTC 1 cut(s) 678
BsmFI GGGAC 1 cut(s) 456
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 2 cut(s) 1056, 1447
Bsp1286I GDGCHC 1 cut(s) 1581
Bsp1407I TGTACA 2 cut(s) 832, 1353
Bsp143I GATC 5 cut(s) 178, 297, 423, 652, 1213
Bsp19I CCATGG 1 cut(s) 263
BspACI CCGC 4 cut(s) 7, 500, 786, 1047
BspANI GGCC 2 cut(s) 1056, 1447
BspCNI CTCAG 2 cut(s) 322, 445
BspFNI CGCG 1 cut(s) 585
BspHI TCATGA 2 cut(s) 823, 1543
BspLI GGNNCC 1 cut(s) 565
BspPI GGATC 2 cut(s) 305, 1208
BspT107I GGYRCC 1 cut(s) 563
BsrDI GCAATG 1 cut(s) 112
BsrGI TGTACA 2 cut(s) 832, 1353
BssECI CCNNGG 4 cut(s) 119, 263, 543, 1248
BssMI GATC 5 cut(s) 178, 297, 423, 652, 1213
BssNI GRCGYC 1 cut(s) 789
BssT1I CCWWGG 3 cut(s) 119, 263, 1248
Bst2UI CCWGG 1 cut(s) 544
Bst4CI ACNGT 3 cut(s) 162, 634, 1133
BstACI GRCGYC 1 cut(s) 789
BstAUI TGTACA 2 cut(s) 832, 1353
BstDEI CTNAG 6 cut(s) 330, 345, 375, 453, 1423, 1440
BstDSI CCRYGG 1 cut(s) 263
BstEII GGTNACC 1 cut(s) 1253
BstF5I GGATG 1 cut(s) 610
BstFNI CGCG 1 cut(s) 585
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 5 cut(s) 181, 300, 426, 655, 1216
BstMAI GTCTC 1 cut(s) 678
BstMBI GATC 5 cut(s) 178, 297, 423, 652, 1213
BstMWI GCNNNNNNNGC 5 cut(s) 376, 932, 1053, 1511, 1520
BstNI CCWGG 1 cut(s) 544
BstPAI GACNNNNGTC 1 cut(s) 1607
BstPI GGTNACC 1 cut(s) 1253
BstSCI CCNGG 4 cut(s) 542, 679, 705, 1537
BstSFI CTRYAG 2 cut(s) 158, 633
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 2 cut(s) 357, 625
BstV2I GAAGAC 4 cut(s) 399, 694, 1010, 1086
BsuRI GGCC 2 cut(s) 1056, 1447
BtgI CCRYGG 1 cut(s) 263
BtgZI GCGATG 2 cut(s) 62, 1166
BtsCI GGATG 1 cut(s) 610
BtsI GCAGTG 1 cut(s) 1350
BtsIMutI CAGTG 2 cut(s) 1350, 1476
CciI TCATGA 2 cut(s) 823, 1543
CfoI GCGC 1 cut(s) 243
CseI GACGC 1 cut(s) 778
Csp6I GTAC 3 cut(s) 833, 1134, 1354
CspCI CAANNNNNGTGG 4 cut(s) 374, 409, 1347, 1382
CviAII CATG 6 cut(s) 145, 238, 264, 824, 1544, 1551
CviQI GTAC 3 cut(s) 833, 1134, 1354
DdeI CTNAG 6 cut(s) 330, 345, 375, 453, 1423, 1440
DpnI GATC 5 cut(s) 180, 299, 425, 654, 1215
DpnII GATC 5 cut(s) 178, 297, 423, 652, 1213
DraI TTTAAA 1 cut(s) 366
DrdI GACNNNNNNGTC 1 cut(s) 1396
DseDI GACNNNNNNGTC 1 cut(s) 1396
EciI GGCGGA 1 cut(s) 489
Ecl136II GAGCTC 1 cut(s) 1579
Eco130I CCWWGG 3 cut(s) 119, 263, 1248
Eco147I AGGCCT 1 cut(s) 1056
Eco24I GRGCYC 1 cut(s) 1581
Eco32I GATATC 1 cut(s) 142
Eco53kI GAGCTC 1 cut(s) 1579
Eco57I CTGAAG 2 cut(s) 47, 300
Eco91I GGTNACC 1 cut(s) 1253
EcoICRI GAGCTC 1 cut(s) 1579
EcoO65I GGTNACC 1 cut(s) 1253
EcoRII CCWGG 1 cut(s) 542
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 3 cut(s) 119, 263, 1248
EcoT22I ATGCAT 1 cut(s) 928
EcoT38I GRGCYC 1 cut(s) 1581
ErhI CCWWGG 3 cut(s) 119, 263, 1248
Esp3I CGTCTC 1 cut(s) 678
FaeI CATG 6 cut(s) 148, 241, 267, 827, 1547, 1554
FaqI GGGAC 1 cut(s) 456
FatI CATG 6 cut(s) 144, 237, 263, 823, 1543, 1550
FauNDI CATATG 1 cut(s) 1236
FbaI TGATCA 2 cut(s) 178, 423
FblI GTMKAC 2 cut(s) 463, 596
Fnu4HI GCNGC 4 cut(s) 8, 371, 639, 787
FokI GGATG 1 cut(s) 617
FriOI GRGCYC 1 cut(s) 1581
Fsp4HI GCNGC 4 cut(s) 8, 371, 639, 787
FspBI CTAG 4 cut(s) 1167, 1302, 1478, 1612
FspI TGCGCA 1 cut(s) 242
GlaI GCGC 1 cut(s) 242
GluI GCNGC 4 cut(s) 8, 371, 639, 787
HaeIII GGCC 2 cut(s) 1056, 1447
HapII CCGG 3 cut(s) 681, 707, 1538
HgaI GACGC 1 cut(s) 778
HhaI GCGC 1 cut(s) 243
Hin1I GRCGYC 1 cut(s) 789
Hin1II CATG 6 cut(s) 148, 241, 267, 827, 1547, 1554
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HincII GTYRAC 1 cut(s) 257
HindII GTYRAC 1 cut(s) 257
HinfI GANTC 9 cut(s) 19, 154, 483, 573, 950, 1492, 1547, 1584, 1603
HpaII CCGG 3 cut(s) 681, 707, 1538
HphI GGTGA 5 cut(s) 313, 1031, 1082, 1136, 1265
Hpy166II GTNNAC 7 cut(s) 257, 464, 597, 773, 835, 1356, 1369
Hpy188I TCNGA 3 cut(s) 454, 657, 1583
Hpy188III TCNNGA 5 cut(s) 480, 824, 1499, 1544, 1574
Hpy8I GTNNAC 7 cut(s) 257, 464, 597, 773, 835, 1356, 1369
Hpy99I CGWCG 3 cut(s) 255, 794, 1157
HpyAV CCTTC 6 cut(s) 31, 180, 334, 1091, 1109, 1304
HpyCH4III ACNGT 3 cut(s) 162, 634, 1133
HpyCH4IV ACGT 5 cut(s) 749, 882, 1177, 1365, 1391
HpyCH4V TGCA 8 cut(s) 641, 675, 818, 926, 1243, 1332, 1343, 1385
HpyF10VI GCNNNNNNNGC 5 cut(s) 376, 932, 1053, 1511, 1520
HpyF3I CTNAG 6 cut(s) 330, 345, 375, 453, 1423, 1440
HpySE526I ACGT 5 cut(s) 749, 882, 1177, 1365, 1391
Hsp92I GRCGYC 1 cut(s) 789
Hsp92II CATG 6 cut(s) 148, 241, 267, 827, 1547, 1554
HspAI GCGC 1 cut(s) 241
Ksp22I TGATCA 2 cut(s) 178, 423
Kzo9I GATC 5 cut(s) 178, 297, 423, 652, 1213
Lsp1109I GCAGC 2 cut(s) 357, 625
LweI GCATC 5 cut(s) 817, 935, 1230, 1273, 1341
MaeI CTAG 4 cut(s) 1167, 1302, 1478, 1612
MaeII ACGT 5 cut(s) 749, 882, 1177, 1365, 1391
MaeIII GTNAC 3 cut(s) 902, 1253, 1304
MalI GATC 5 cut(s) 180, 299, 425, 654, 1215
MboI GATC 5 cut(s) 178, 297, 423, 652, 1213
MfeI CAATTG 2 cut(s) 219, 1259
MhlI GDGCHC 1 cut(s) 1581
MlyI GAGTC 4 cut(s) 492, 1556, 1593, 1597
MnlI CCTC 6 cut(s) 207, 383, 731, 1069, 1437, 1525
Mph1103I ATGCAT 1 cut(s) 928
MseI TTAA 5 cut(s) 269, 365, 588, 809, 974
MspI CCGG 3 cut(s) 681, 707, 1538
MspR9I CCNGG 4 cut(s) 544, 681, 707, 1539
MunI CAATTG 2 cut(s) 219, 1259
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 1 cut(s) 544
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 5 cut(s) 376, 932, 1053, 1511, 1520
NciI CCSGG 3 cut(s) 681, 707, 1539
NcoI CCATGG 1 cut(s) 263
NdeI CATATG 1 cut(s) 1236
NdeII GATC 5 cut(s) 178, 297, 423, 652, 1213
NlaIII CATG 6 cut(s) 148, 241, 267, 827, 1547, 1554
NlaIV GGNNCC 1 cut(s) 565
NmeAIII GCCGAG 1 cut(s) 1592
NmuCI GTSAC 1 cut(s) 1253
NsbI TGCGCA 1 cut(s) 242
NsiI ATGCAT 1 cut(s) 928
PagI TCATGA 2 cut(s) 823, 1543
PceI AGGCCT 1 cut(s) 1056
PctI GAATGC 1 cut(s) 664
PfeI GAWTC 5 cut(s) 19, 154, 573, 950, 1492
PflMI CCANNNNNTGG 1 cut(s) 549
PkrI GCNGC 4 cut(s) 9, 372, 640, 788
PleI GAGTC 4 cut(s) 491, 1555, 1592, 1597
PpsI GAGTC 4 cut(s) 491, 1555, 1592, 1597
PshAI GACNNNNGTC 1 cut(s) 1607
Psp124BI GAGCTC 1 cut(s) 1581
Psp6I CCWGG 1 cut(s) 542
PspEI GGTNACC 1 cut(s) 1253
PspGI CCWGG 1 cut(s) 542
PspN4I GGNNCC 1 cut(s) 565
RsaI GTAC 3 cut(s) 834, 1135, 1355
RsaNI GTAC 3 cut(s) 833, 1134, 1354
SacI GAGCTC 1 cut(s) 1581
SaqAI TTAA 5 cut(s) 269, 365, 588, 809, 974
SatI GCNGC 4 cut(s) 8, 371, 639, 787
Sau3AI GATC 5 cut(s) 178, 297, 423, 652, 1213
ScaI AGTACT 1 cut(s) 1135
SchI GAGTC 4 cut(s) 492, 1556, 1593, 1597
ScrFI CCNGG 4 cut(s) 544, 681, 707, 1539
SduI GDGCHC 1 cut(s) 1581
SfaNI GCATC 5 cut(s) 817, 935, 1230, 1273, 1341
SfcI CTRYAG 2 cut(s) 158, 633
SmlI CTYRAG 2 cut(s) 310, 505
SmoI CTYRAG 2 cut(s) 310, 505
SpeI ACTAGT 1 cut(s) 1477
SseBI AGGCCT 1 cut(s) 1056
SsiI CCGC 4 cut(s) 7, 500, 786, 1047
SspMI CTAG 4 cut(s) 1167, 1302, 1478, 1612
SstI GAGCTC 1 cut(s) 1581
StuI AGGCCT 1 cut(s) 1056
StyD4I CCNGG 4 cut(s) 542, 679, 705, 1537
StyI CCWWGG 3 cut(s) 119, 263, 1248
TaaI ACNGT 3 cut(s) 162, 634, 1133
TaiI ACGT 5 cut(s) 752, 885, 1180, 1368, 1394
TaqI TCGA 4 cut(s) 168, 648, 1155, 1573
TatI WGTACW 3 cut(s) 832, 1133, 1353
TauI GCSGC 2 cut(s) 10, 789
TfiI GAWTC 5 cut(s) 19, 154, 573, 950, 1492
Tru1I TTAA 5 cut(s) 269, 365, 588, 809, 974
Tru9I TTAA 5 cut(s) 269, 365, 588, 809, 974
TscAI CASTG 2 cut(s) 1350, 1476
TseFI GTSAC 1 cut(s) 1253
TseI GCWGC 2 cut(s) 370, 638
Tsp45I GTSAC 1 cut(s) 1253
TspDTI ATGAA 7 cut(s) 226, 906, 1038, 1128, 1158, 1223, 1484
TspRI CASTG 2 cut(s) 1350, 1476
Van91I CCANNNNNTGG 1 cut(s) 549
XapI RAATTY 1 cut(s) 1228
XmiI GTMKAC 2 cut(s) 463, 596
XspI CTAG 4 cut(s) 1167, 1302, 1478, 1612
ZrmI AGTACT 1 cut(s) 1135
Zsp2I ATGCAT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.