Rh4AG020900

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000144
Physical Location & Seq
Forward (+)
3940406 .. 3948331
7926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG020900.1

Sequence Viewer

Length: 849 bp
ATGATTGTGCCACACCTGAGTGTACGTGATCGTATACGATTAAGCAGTGTTTGCAAGCCTTGGAGCTCAGTTCCTATGCGCACCGACATCCCTAGTGCTCAACATCAGCTTCCCTGGTTAATACTCCCTCAATCTTGCCATAGCTACTTGAGCTTTTTCGACCTCTGTGAAGGTAAAGTTGGCAAGTTGGACCTGCCTGAACCAGTTCAAGGAGGGTGGTTTCAAGGATCATCAAAAGGTTGGATGATTATGATCAAGGAAAAGGATCTGGATTCTACAATGTTACTTGTGAATCCAATCTCAGGAGCCCAGCACCAACTTCCACCTTTGAGCACGACTCCATCTTTCCAGAGCTTTGTAGACACAACCGCATGGAAACGCTATGGTGCTTCTGCTTTCTGCTATAGCGTTGTGTTGTGTACCTCAGATATTGATTCAGATCAGTTTATGGTTGCAGCAACTTTTGGAGATACACTGTGTTTGTGTAGACTGGGGGACAAGAGGCGGAGGTTCATCGAGGTGTTAGATGAAAACGAGGGCATTACAGATTTATTGTTTTCTTCTGGGAAGCTCTACGCCTTGCTTCTTAGTGATAATAAGATTGGCGTCATCTCAGCTCCTCGCACCTTAAAATGTGGAGATCATGTATTGGAATTGCAGCTGGTCTATGACAAAAAACCAAGAACCAATGACAGCATGGAAGAATATGCTGACTATCTAATCCAATTACAAGGGACAGTCGTAGTTCTTCCATTATTTCTTTGCCTTGGATTGCGGATCCCTACACCTTGGGTATCATCTCCACTTGTCAAGTTTATGATTAGATTGCTAATTATCTTAGCCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

282

Amino Acids

31.63

Weight (kDa)

6.21

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 52 - 249 1e-20 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 201
AccI GTMKAC 3 cut(s) 34, 360, 487
AciI CCGC 3 cut(s) 369, 505, 775
AclWI GGATC 4 cut(s) 235, 273, 772, 785
AcyI GRCGYC 1 cut(s) 606
AfaI GTAC 2 cut(s) 24, 421
AfiI CCNNNNNNNGG 2 cut(s) 209, 302
AgsI TTSAA 2 cut(s) 209, 224
AjnI CCWGG 1 cut(s) 113
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AleI CACNNNNGTG 1 cut(s) 18
AluBI AGCT 8 cut(s) 66, 109, 144, 153, 354, 571, 617, 661
AluI AGCT 8 cut(s) 66, 109, 144, 153, 354, 571, 617, 661
Alw21I GWGCWC 3 cut(s) 68, 100, 335
AlwI GGATC 4 cut(s) 235, 273, 772, 785
ApeKI GCWGC 2 cut(s) 455, 658
Asp700I GAANNNNTTC 1 cut(s) 204
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 190
AvaII GGWCC 1 cut(s) 190
BamHI GGATCC 1 cut(s) 777
BanII GRGCYC 2 cut(s) 68, 310
Bbv12I GWGCWC 3 cut(s) 68, 100, 335
BbvI GCAGC 2 cut(s) 467, 670
BccI CCATC 1 cut(s) 349
BciT130I CCWGG 1 cut(s) 115
BclI TGATCA 1 cut(s) 252
BfaI CTAG 2 cut(s) 93, 847
BfmI CTRYAG 1 cut(s) 403
BfuAI ACCTGC 1 cut(s) 201
BisI GCNGC 2 cut(s) 456, 659
BlsI GCNGC 2 cut(s) 457, 660
Bme1390I CCNGG 1 cut(s) 115
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 2 cut(s) 307, 779
BmrFI CCNGG 1 cut(s) 115
BmrI ACTGGG 1 cut(s) 500
BmuI ACTGGG 1 cut(s) 500
BplI GAGNNNNNCTC 2 cut(s) 322, 354
BpuEI CTTGAG 1 cut(s) 169
BsaAI YACGTR 1 cut(s) 26
BsaBI GATNNNNATC 2 cut(s) 251, 438
BsaHI GRCGYC 1 cut(s) 606
BsaJI CCNNGG 4 cut(s) 59, 113, 766, 788
BsaXI ACNNNNNCTCC 2 cut(s) 630, 660
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 302
Bse1I ACTGG 2 cut(s) 203, 495
Bse8I GATNNNNATC 2 cut(s) 251, 438
BseBI CCWGG 1 cut(s) 115
BseDI CCNNGG 4 cut(s) 59, 113, 766, 788
BseGI GGATG 2 cut(s) 87, 249
BseJI GATNNNNATC 2 cut(s) 251, 438
BseLI CCNNNNNNNGG 2 cut(s) 209, 302
BseMII CTCAG 5 cut(s) 8, 81, 315, 438, 627
BseNI ACTGG 2 cut(s) 203, 495
BseRI GAGGAG 1 cut(s) 609
BseXI GCAGC 2 cut(s) 467, 670
BseYI CCCAGC 1 cut(s) 309
BsiHKAI GWGCWC 3 cut(s) 68, 100, 335
BslFI GGGAC 2 cut(s) 509, 748
BslI CCNNNNNNNGG 2 cut(s) 209, 302
BsmFI GGGAC 2 cut(s) 509, 748
Bsp1286I GDGCHC 4 cut(s) 68, 100, 310, 335
Bsp143I GATC 7 cut(s) 28, 227, 252, 265, 439, 640, 777
BspACI CCGC 3 cut(s) 369, 505, 775
BspCNI CTCAG 5 cut(s) 9, 80, 314, 437, 626
BspLI GGNNCC 2 cut(s) 307, 779
BspMI ACCTGC 1 cut(s) 201
BspPI GGATC 4 cut(s) 235, 273, 772, 785
BsrI ACTGG 2 cut(s) 203, 495
BssECI CCNNGG 4 cut(s) 59, 113, 766, 788
BssMI GATC 7 cut(s) 28, 227, 252, 265, 439, 640, 777
BssNAI GTATAC 1 cut(s) 35
BssNI GRCGYC 1 cut(s) 606
BssT1I CCWWGG 3 cut(s) 59, 766, 788
Bst1107I GTATAC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 115
Bst4CI ACNGT 2 cut(s) 477, 739
BstACI GRCGYC 1 cut(s) 606
BstAPI GCANNNNNTGC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 7 cut(s) 17, 67, 301, 424, 587, 613, 838
BstF5I GGATG 2 cut(s) 87, 249
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 7 cut(s) 31, 230, 255, 268, 442, 643, 780
BstMBI GATC 7 cut(s) 28, 227, 252, 265, 439, 640, 777
BstMWI GCNNNNNNNGC 2 cut(s) 51, 150
BstNI CCWGG 1 cut(s) 115
BstSCI CCNGG 1 cut(s) 113
BstSFI CTRYAG 1 cut(s) 403
BstV1I GCAGC 2 cut(s) 467, 670
BstX2I RGATCY 2 cut(s) 265, 777
BstYI RGATCY 2 cut(s) 265, 777
BstZ17I GTATAC 1 cut(s) 35
BtsCI GGATG 2 cut(s) 87, 249
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 2 cut(s) 52, 473
BveI ACCTGC 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 56
CfoI GCGC 1 cut(s) 81
Cfr13I GGNCC 1 cut(s) 190
CseI GACGC 1 cut(s) 595
Csp6I GTAC 2 cut(s) 23, 420
CspCI CAANNNNNGTGG 2 cut(s) 197, 232
CviAII CATG 3 cut(s) 372, 644, 697
CviQI GTAC 2 cut(s) 23, 420
DdeI CTNAG 7 cut(s) 17, 67, 301, 424, 587, 613, 838
DpnI GATC 7 cut(s) 30, 229, 254, 267, 441, 642, 779
DpnII GATC 7 cut(s) 28, 227, 252, 265, 439, 640, 777
EciI GGCGGA 1 cut(s) 520
Ecl136II GAGCTC 1 cut(s) 66
Eco130I CCWWGG 3 cut(s) 59, 766, 788
Eco24I GRGCYC 2 cut(s) 68, 310
Eco47I GGWCC 1 cut(s) 190
Eco53kI GAGCTC 1 cut(s) 66
EcoICRI GAGCTC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 113
EcoT14I CCWWGG 3 cut(s) 59, 766, 788
EcoT38I GRGCYC 2 cut(s) 68, 310
ErhI CCWWGG 3 cut(s) 59, 766, 788
FaeI CATG 3 cut(s) 375, 647, 700
FaqI GGGAC 2 cut(s) 509, 748
FatI CATG 3 cut(s) 371, 643, 696
FbaI TGATCA 1 cut(s) 252
FblI GTMKAC 3 cut(s) 34, 360, 487
Fnu4HI GCNGC 2 cut(s) 456, 659
FokI GGATG 2 cut(s) 74, 256
FriOI GRGCYC 2 cut(s) 68, 310
Fsp4HI GCNGC 2 cut(s) 456, 659
FspAI RTGCGCAY 1 cut(s) 80
FspBI CTAG 2 cut(s) 93, 847
FspI TGCGCA 1 cut(s) 80
GlaI GCGC 1 cut(s) 80
GluI GCNGC 2 cut(s) 456, 659
GsaI CCCAGC 1 cut(s) 313
HgaI GACGC 1 cut(s) 595
HhaI GCGC 1 cut(s) 81
Hin1I GRCGYC 1 cut(s) 606
Hin1II CATG 3 cut(s) 375, 647, 700
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HinfI GANTC 4 cut(s) 272, 292, 337, 434
Hpy166II GTNNAC 5 cut(s) 23, 35, 361, 420, 488
Hpy188I TCNGA 2 cut(s) 427, 439
Hpy188III TCNNGA 3 cut(s) 269, 303, 349
Hpy8I GTNNAC 5 cut(s) 23, 35, 361, 420, 488
HpyAV CCTTC 1 cut(s) 164
HpyCH4III ACNGT 2 cut(s) 477, 739
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 3 cut(s) 54, 455, 658
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 150
HpyF3I CTNAG 7 cut(s) 17, 67, 301, 424, 587, 613, 838
HpySE526I ACGT 1 cut(s) 25
Hsp92I GRCGYC 1 cut(s) 606
Hsp92II CATG 3 cut(s) 375, 647, 700
HspAI GCGC 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 252
Kzo9I GATC 7 cut(s) 28, 227, 252, 265, 439, 640, 777
LmnI GCTCC 3 cut(s) 63, 305, 622
Lsp1109I GCAGC 2 cut(s) 467, 670
MaeI CTAG 2 cut(s) 93, 847
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 1 cut(s) 282
MalI GATC 7 cut(s) 30, 229, 254, 267, 441, 642, 779
MboI GATC 7 cut(s) 28, 227, 252, 265, 439, 640, 777
MboII GAAGA 3 cut(s) 552, 713, 740
MflI RGATCY 2 cut(s) 265, 777
MhlI GDGCHC 4 cut(s) 68, 100, 310, 335
MluCI AATT 3 cut(s) 653, 725, 831
MlyI GAGTC 1 cut(s) 331
MmeI TCCRAC 2 cut(s) 168, 221
MnlI CCTC 9 cut(s) 138, 173, 206, 433, 495, 501, 511, 529, 630
MroXI GAANNNNTTC 1 cut(s) 204
MseI TTAA 3 cut(s) 41, 119, 629
MslI CAYNNNNRTG 2 cut(s) 18, 518
MspA1I CMGCKG 1 cut(s) 661
MspR9I CCNGG 1 cut(s) 115
MvaI CCWGG 1 cut(s) 115
MwoI GCNNNNNNNGC 2 cut(s) 51, 150
NdeII GATC 7 cut(s) 28, 227, 252, 265, 439, 640, 777
NlaIII CATG 3 cut(s) 375, 647, 700
NlaIV GGNNCC 2 cut(s) 307, 779
NsbI TGCGCA 1 cut(s) 80
OliI CACNNNNGTG 1 cut(s) 18
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 3 cut(s) 272, 292, 434
PkrI GCNGC 2 cut(s) 457, 660
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
Ppu21I YACGTR 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 309
PspGI CCWGG 1 cut(s) 113
PspN4I GGNNCC 2 cut(s) 307, 779
PspPI GGNCC 1 cut(s) 190
PsuI RGATCY 2 cut(s) 265, 777
PvuII CAGCTG 1 cut(s) 661
RsaI GTAC 2 cut(s) 24, 421
RsaNI GTAC 2 cut(s) 23, 420
RseI CAYNNNNRTG 2 cut(s) 18, 518
SacI GAGCTC 1 cut(s) 68
SaqAI TTAA 3 cut(s) 41, 119, 629
SatI GCNGC 2 cut(s) 456, 659
Sau3AI GATC 7 cut(s) 28, 227, 252, 265, 439, 640, 777
Sau96I GGNCC 1 cut(s) 190
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 1 cut(s) 115
SduI GDGCHC 4 cut(s) 68, 100, 310, 335
SfcI CTRYAG 1 cut(s) 403
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 18, 518
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 3 cut(s) 653, 725, 831
SsiI CCGC 3 cut(s) 369, 505, 775
SspMI CTAG 2 cut(s) 93, 847
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 1 cut(s) 113
StyI CCWWGG 3 cut(s) 59, 766, 788
TaaI ACNGT 2 cut(s) 477, 739
TaiI ACGT 1 cut(s) 28
TaqI TCGA 2 cut(s) 159, 516
TasI AATT 3 cut(s) 653, 725, 831
TfiI GAWTC 3 cut(s) 272, 292, 434
Tru1I TTAA 3 cut(s) 41, 119, 629
Tru9I TTAA 3 cut(s) 41, 119, 629
TscAI CASTG 2 cut(s) 52, 480
TseI GCWGC 2 cut(s) 455, 658
TspDTI ATGAA 2 cut(s) 502, 543
TspRI CASTG 2 cut(s) 52, 480
VpaK11BI GGWCC 1 cut(s) 190
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmiI GTMKAC 3 cut(s) 34, 360, 487
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 2 cut(s) 93, 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.