FvH4_2g16551

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000144
Physical Location & Seq
Reverse (-)
14427987 .. 14434545
6559 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g16551.t1

Sequence Viewer

Length: 2145 bp
ATGAATGAGATGATTCAGACTCGTTCCGCGACAAAGAGAAACAGACAACAGAACATATCGACCAGCGTCGACGACCAAACTTGTTATGGGTGCTGGCAGCATCCACCAGAAGATATCATGCAAAAAATATTACAACATATGAGCTTAGATGATCGTTTACGCTTAAGCTTCGTGTGCAAGTACTGGCAGTCGTTTGCTACTCGAAGAGACATGCCCAGTGGTGGACAACTCCCCTGGTTAGTGCTCCCTCAACCCCCAAATTCTCAGTTCTTGAGCTTTGCCAACCTCTCTAAGCGCGAAGTTGTGTGGTTGAATATGCCTAAGCCAGTTAAAGGCGTGTGTTTTCATGCTTCTTCCAGAGGTTGGTTGATTCTGTTTAGAGGAAGACGACCAGAAGGTCATATGTTTATGATAAATCCACTTTCTGGAGTTCAACACAACCTTCCGGCCTTGAAAACAATTGTAACTTTCCGAATCGGTTTTAGGAAATGGCGTGAACCCCAAAACTTTGTTACTAAAATTGCACTGTCTAGTCCAAATACCTTAAAATGTATAGTGGCAGCATTTTTTAGTAGTCATGAGTTAGGTTTTTGCAGACCTGGAGATAAAAGATGGATTGTCTTCGAGCTTAAGGACAATTACATTGGTGTCACTGATATATTCTTCTCAGGTACCACGCTCTACGCCTTGCTTCGTCATAACTATGCTAGCGAACGCTGCACAGTTGTATCTTACCCCATAACTTTTGAAGATCATGTCGTGGAATTGAAGTTGATCTATGACGAAATAGCAAGCTTCAACAGCATATCTTACATGATAGAATCATCTATCAACAATGAAGTCTTGCTCATCCATCAAATGCTAGATCGCACATTCATCAATGATGAGCCGGGAAGCCGAATATCGTCCGTGACGACGAGTGGTTTCATTGTATACAAGATTGACGTCGAGTGTGGCAACTTCTTGAGGGTACAAAGTTTGGAGGACGAAACATTGTTTTTGGCCGAAAATGGTTCTTCATTGTCCTTTCGAGCCAGTGATCACGAAGAATTACTAGGAAACTGCATTTATTTTGTACAACATGAAACGATCAGCATTTTATATTTGGACACTGGAAAAACTGAGAGATTGTTTCCAGATGTTGTCAACCTGGAGGGTCAGAAGGGGTGGTTCACTCCAAGTTTATGGAGACCACCTGAAGATGTCAAAGAGATGATCGTTAAGCACATGAGTTTCAGTGGTCGAGTTCGCTTAAGCAGTTTGTGCAAGTCTTGGAGGTCAGTTTGTATGCGAAGAGATATCCCGACTTGTCCACAACTCCCATGGTTAGTACACCCTCAAGCCAAAACAGGAGATAGCTTGAGCTTCTCTAGCCTTTCAGAGGGCAAAGTTTTCAAGTCGAGGCTGCCTTGCCCTGATTTCAAGTCGAGGCTGGAAAGGCTGCTAGTTGGGACAAGTACTAATCGGACATCTTCTTTTTTCGAGTCTTCCAGAGGAGCCCAGCATCGACTTCCTCCTTTACAAACAATTCCTGGCTATAAAGACAATTCTGACGATTCTCTCCTTGGTCAAGTTAAATTATCTAGCCCAGATGTAGCAACATGTATGGTGGCAGCAACTCTTGAAGGAGAGATTTGCAGGGCATACTTGTCAGAATCAACAAACAATGAACTTATGTTGACTTATCAAATTTATGGCAATGATGTTGGTGACGATGACAATAACAACAACGGAGAAGGAGATGAGAATGACATGGAAGGTAATGAGCAGAACGAGAACGTTCTAGGAGGCAACCCTAGACCCTTCAATGGTCAATTTAATGCTGCAAGGCCCTTCAACAGTAGCTTTGGTGGGATGAACAACAATGCAGGATTCAAAGGTGGCTTCAATCCAAACTTTGGTGGGATGAATAATAATGCAGGATTCAATGGTGGTTTCATCAATTCAGGCTTCAACAATGGAGCAAACAACTCTCTGACATGTCAATTCTGCAACAAAGTTAGTCATGGTGCAAGAGCTTGCCGAGCTCTAGCCAATATGCAAAGGGGAGGAGGCAATAATAGAAACTTTGGCAATAATGGAGGTTTCAGGAGCAACTCTGGAAACAACCAGAACTTTGAAGATTGCATATATACTGTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

715

Amino Acids

80.26

Weight (kDa)

7.19

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 36 - 68 2.1e-08 F-box domain
F-box-like PF12937 36 - 67 2.7e-06 F-box-like
Beta-prop_KIB1-4 PF03478 94 - 360 2e-36 KIB1-4 beta-propeller
F-box PF00646 399 - 432 2.3e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 396
AatII GACGTC 1 cut(s) 948
Acc65I GGTACC 1 cut(s) 671
AccB1I GGYRCC 1 cut(s) 671
AccB7I CCANNNNNTGG 3 cut(s) 363, 425, 1898
AccI GTMKAC 2 cut(s) 69, 933
AccII CGCG 2 cut(s) 29, 297
AciI CCGC 1 cut(s) 27
AclI AACGTT 1 cut(s) 1779
AcoI YGGCCR 1 cut(s) 1002
AcsI RAATTY 2 cut(s) 259, 1689
AcuI CTGAAG 1 cut(s) 1218
AcyI GRCGYC 1 cut(s) 945
AfaI GTAC 6 cut(s) 182, 673, 972, 1077, 1332, 1459
AfiI CCNNNNNNNGG 7 cut(s) 221, 332, 363, 425, 1381, 1808, 1898
AflII CTTAAG 3 cut(s) 163, 629, 1252
AflIII ACRYGT 2 cut(s) 1601, 1979
AjnI CCWGG 4 cut(s) 233, 598, 1149, 1531
Alw21I GWGCWC 2 cut(s) 246, 2029
Alw26I GTCTC 2 cut(s) 201, 1183
AoxI GGCC 3 cut(s) 447, 1002, 1829
ApeKI GCWGC 7 cut(s) 97, 560, 717, 1405, 1441, 1613, 1823
ApoI RAATTY 2 cut(s) 259, 1689
Asp718I GGTACC 1 cut(s) 671
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 1830
AsuC2I CCSGG 1 cut(s) 891
AsuHPI GGTGA 1 cut(s) 1721
AsuNHI GCTAGC 1 cut(s) 707
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BanI GGYRCC 1 cut(s) 671
BanII GRGCYC 2 cut(s) 1501, 2029
BbsI GAAGAC 3 cut(s) 391, 613, 1479
Bbv12I GWGCWC 2 cut(s) 246, 2029
BbvI GCAGC 7 cut(s) 109, 572, 704, 1392, 1428, 1625, 1810
BccI CCATC 2 cut(s) 606, 861
BciT130I CCWGG 4 cut(s) 235, 600, 1151, 1533
BclI TGATCA 1 cut(s) 1039
BcnI CCSGG 1 cut(s) 891
BcoDI GTCTC 2 cut(s) 201, 1183
BfrI CTTAAG 3 cut(s) 163, 629, 1252
BisI GCNGC 7 cut(s) 98, 561, 718, 1406, 1442, 1614, 1824
BlsI GCNGC 7 cut(s) 99, 562, 719, 1407, 1443, 1615, 1825
BmcAI AGTACT 2 cut(s) 182, 1459
Bme1390I CCNGG 5 cut(s) 235, 600, 891, 1151, 1533
BmgT120I GGNCC 1 cut(s) 1830
BmiI GGNNCC 2 cut(s) 673, 1498
BmrFI CCNGG 5 cut(s) 235, 600, 891, 1151, 1533
BmrI ACTGGG 1 cut(s) 210
BmsI GCATC 2 cut(s) 109, 1513
BmtI GCTAGC 1 cut(s) 711
BmuI ACTGGG 1 cut(s) 210
BoxI GACNNNNGTC 1 cut(s) 65
BpiI GAAGAC 3 cut(s) 391, 613, 1479
BpmI CTGGAG 3 cut(s) 447, 621, 1172
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 4 cut(s) 292, 985, 1323, 1381
BpuMI CCSGG 1 cut(s) 891
BsaHI GRCGYC 1 cut(s) 945
BsaI GGTCTC 1 cut(s) 1183
BsaJI CCNNGG 3 cut(s) 233, 1322, 1564
Bsc4I CCNNNNNNNGG 7 cut(s) 221, 332, 363, 425, 1381, 1808, 1898
Bse1I ACTGG 5 cut(s) 188, 216, 326, 1035, 1117
Bse3DI GCAATG 1 cut(s) 1705
BseBI CCWGG 4 cut(s) 235, 600, 1151, 1533
BseDI CCNNGG 3 cut(s) 233, 1322, 1564
BseGI GGATG 4 cut(s) 100, 849, 1860, 1911
BseLI CCNNNNNNNGG 7 cut(s) 221, 332, 363, 425, 1381, 1808, 1898
BseMI GCAATG 1 cut(s) 1705
BseMII CTCAG 3 cut(s) 278, 681, 1113
BseNI ACTGG 5 cut(s) 188, 216, 326, 1035, 1117
BseRI GAGGAG 2 cut(s) 1509, 2064
BseXI GCAGC 7 cut(s) 109, 572, 704, 1392, 1428, 1625, 1810
BseYI CCCAGC 1 cut(s) 1500
BsgI GTGCAG 1 cut(s) 703
Bsh1236I CGCG 2 cut(s) 29, 297
BshFI GGCC 3 cut(s) 449, 1004, 1831
BshNI GGYRCC 1 cut(s) 671
BsiHKAI GWGCWC 2 cut(s) 246, 2029
BsiSI CCGG 2 cut(s) 446, 890
BslFI GGGAC 1 cut(s) 1465
BslI CCNNNNNNNGG 7 cut(s) 221, 332, 363, 425, 1381, 1808, 1898
BsmAI GTCTC 2 cut(s) 201, 1183
BsmFI GGGAC 1 cut(s) 1465
BsnI GGCC 3 cut(s) 449, 1004, 1831
Bso31I GGTCTC 1 cut(s) 1183
Bsp1286I GDGCHC 3 cut(s) 246, 1501, 2029
Bsp1407I TGTACA 1 cut(s) 1075
Bsp143I GATC 7 cut(s) 151, 751, 774, 865, 1039, 1089, 1215
Bsp19I CCATGG 1 cut(s) 1322
BspACI CCGC 1 cut(s) 27
BspANI GGCC 3 cut(s) 449, 1004, 1831
BspCNI CTCAG 3 cut(s) 277, 680, 1114
BspFNI CGCG 2 cut(s) 29, 297
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 2 cut(s) 673, 1498
BspOI GCTAGC 1 cut(s) 711
BspT107I GGYRCC 1 cut(s) 671
BspTI CTTAAG 3 cut(s) 163, 629, 1252
BspTNI GGTCTC 1 cut(s) 1183
BsrDI GCAATG 1 cut(s) 1705
BsrGI TGTACA 1 cut(s) 1075
BsrI ACTGG 5 cut(s) 188, 216, 326, 1035, 1117
BssECI CCNNGG 3 cut(s) 233, 1322, 1564
BssMI GATC 7 cut(s) 151, 751, 774, 865, 1039, 1089, 1215
BssNAI GTATAC 1 cut(s) 934
BssNI GRCGYC 1 cut(s) 945
BssT1I CCWWGG 2 cut(s) 1322, 1564
Bst1107I GTATAC 1 cut(s) 934
Bst2UI CCWGG 4 cut(s) 235, 600, 1151, 1533
Bst4CI ACNGT 4 cut(s) 528, 724, 1841, 2137
Bst6I CTCTTC 2 cut(s) 199, 1288
BstACI GRCGYC 1 cut(s) 945
BstAFI CTTAAG 3 cut(s) 163, 629, 1252
BstAPI GCANNNNNTGC 1 cut(s) 1263
BstAUI TGTACA 1 cut(s) 1075
BstC8I GCNNGC 4 cut(s) 95, 709, 793, 2020
BstDEI CTNAG 6 cut(s) 145, 264, 291, 321, 667, 1122
BstDSI CCRYGG 1 cut(s) 1322
BstENI CCTNNNNNAGG 1 cut(s) 1379
BstF5I GGATG 4 cut(s) 100, 849, 1860, 1911
BstFNI CGCG 2 cut(s) 29, 297
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 7 cut(s) 154, 754, 777, 868, 1042, 1092, 1218
BstMAI GTCTC 2 cut(s) 201, 1183
BstMBI GATC 7 cut(s) 151, 751, 774, 865, 1039, 1089, 1215
BstMWI GCNNNNNNNGC 7 cut(s) 174, 717, 801, 1263, 1371, 1438, 2024
BstNI CCWGG 4 cut(s) 235, 600, 1151, 1533
BstNSI RCATGY 3 cut(s) 214, 1605, 1983
BstPAI GACNNNNGTC 1 cut(s) 65
BstSCI CCNGG 5 cut(s) 233, 598, 889, 1149, 1531
BstUI CGCG 2 cut(s) 29, 297
BstV1I GCAGC 7 cut(s) 109, 572, 704, 1392, 1428, 1625, 1810
BstV2I GAAGAC 3 cut(s) 391, 613, 1479
BstXI CCANNNNNNTGG 1 cut(s) 1185
BstZ17I GTATAC 1 cut(s) 934
BsuRI GGCC 3 cut(s) 449, 1004, 1831
BtgI CCRYGG 1 cut(s) 1322
BtsCI GGATG 4 cut(s) 100, 849, 1860, 1911
BtsIMutI CAGTG 6 cut(s) 223, 524, 651, 1042, 1110, 1243
Cac8I GCNNGC 4 cut(s) 95, 709, 793, 2020
CciI TCATGA 1 cut(s) 577
CfoI GCGC 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 1830
CseI GACGC 1 cut(s) 55
Csp6I GTAC 6 cut(s) 181, 672, 971, 1076, 1331, 1458
CviQI GTAC 6 cut(s) 181, 672, 971, 1076, 1331, 1458
DdeI CTNAG 6 cut(s) 145, 264, 291, 321, 667, 1122
DpnI GATC 7 cut(s) 153, 753, 776, 867, 1041, 1091, 1217
DpnII GATC 7 cut(s) 151, 751, 774, 865, 1039, 1089, 1215
DrdI GACNNNNNNGTC 1 cut(s) 396
DseDI GACNNNNNNGTC 1 cut(s) 396
EaeI YGGCCR 1 cut(s) 1002
Eam1104I CTCTTC 2 cut(s) 199, 1288
EarI CTCTTC 2 cut(s) 199, 1288
Ecl136II GAGCTC 1 cut(s) 2027
Eco130I CCWWGG 2 cut(s) 1322, 1564
Eco24I GRGCYC 2 cut(s) 1501, 2029
Eco31I GGTCTC 1 cut(s) 1183
Eco32I GATATC 2 cut(s) 115, 1300
Eco53kI GAGCTC 1 cut(s) 2027
Eco57I CTGAAG 1 cut(s) 1218
EcoICRI GAGCTC 1 cut(s) 2027
EcoNI CCTNNNNNAGG 1 cut(s) 1379
EcoO109I RGGNCCY 1 cut(s) 1830
EcoRII CCWGG 4 cut(s) 233, 598, 1149, 1531
EcoRV GATATC 2 cut(s) 115, 1300
EcoT14I CCWWGG 2 cut(s) 1322, 1564
EcoT38I GRGCYC 2 cut(s) 1501, 2029
ErhI CCWWGG 2 cut(s) 1322, 1564
FaqI GGGAC 1 cut(s) 1465
FauNDI CATATG 2 cut(s) 138, 402
FbaI TGATCA 1 cut(s) 1039
FblI GTMKAC 2 cut(s) 69, 933
Fnu4HI GCNGC 7 cut(s) 98, 561, 718, 1406, 1442, 1614, 1824
FokI GGATG 4 cut(s) 87, 836, 1867, 1918
FriOI GRGCYC 2 cut(s) 1501, 2029
Fsp4HI GCNGC 7 cut(s) 98, 561, 718, 1406, 1442, 1614, 1824
GlaI GCGC 1 cut(s) 296
GluI GCNGC 7 cut(s) 98, 561, 718, 1406, 1442, 1614, 1824
GsaI CCCAGC 1 cut(s) 1504
GsuI CTGGAG 3 cut(s) 447, 621, 1172
HaeIII GGCC 3 cut(s) 449, 1004, 1831
HapII CCGG 2 cut(s) 446, 890
HgaI GACGC 1 cut(s) 55
HhaI GCGC 1 cut(s) 297
Hin1I GRCGYC 1 cut(s) 945
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HincII GTYRAC 3 cut(s) 70, 1147, 1680
HindII GTYRAC 3 cut(s) 70, 1147, 1680
HindIII AAGCTT 2 cut(s) 166, 793
HpaII CCGG 2 cut(s) 446, 890
HphI GGTGA 1 cut(s) 1721
Hpy188I TCNGA 8 cut(s) 18, 473, 1161, 1381, 1467, 1552, 1654, 1977
Hpy99I CGWCG 4 cut(s) 71, 74, 919, 950
HpyAV CCTTC 8 cut(s) 389, 452, 1156, 1619, 1730, 1751, 1813, 1843
HpyCH4III ACNGT 4 cut(s) 528, 724, 1841, 2137
HpyCH4IV ACGT 2 cut(s) 945, 1779
HpyF10VI GCNNNNNNNGC 7 cut(s) 174, 717, 801, 1263, 1371, 1438, 2024
HpyF3I CTNAG 6 cut(s) 145, 264, 291, 321, 667, 1122
HpySE526I ACGT 2 cut(s) 945, 1779
Hsp92I GRCGYC 1 cut(s) 945
HspAI GCGC 1 cut(s) 295
KpnI GGTACC 1 cut(s) 675
Ksp22I TGATCA 1 cut(s) 1039
Kzo9I GATC 7 cut(s) 151, 751, 774, 865, 1039, 1089, 1215
LmnI GCTCC 4 cut(s) 249, 1496, 1961, 2091
Lsp1109I GCAGC 7 cut(s) 109, 572, 704, 1392, 1428, 1625, 1810
LweI GCATC 2 cut(s) 109, 1513
MaeII ACGT 2 cut(s) 945, 1779
MaeIII GTNAC 5 cut(s) 463, 511, 649, 910, 1709
MalI GATC 7 cut(s) 153, 753, 776, 867, 1041, 1091, 1217
MboI GATC 7 cut(s) 151, 751, 774, 865, 1039, 1089, 1215
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 3 cut(s) 246, 1501, 2029
MlyI GAGTC 2 cut(s) 13, 1493
MseI TTAA 8 cut(s) 164, 330, 545, 630, 1221, 1253, 1575, 1818
MslI CAYNNNNRTG 1 cut(s) 702
MspCI CTTAAG 3 cut(s) 163, 629, 1252
MspI CCGG 2 cut(s) 446, 890
MspR9I CCNGG 5 cut(s) 235, 600, 891, 1151, 1533
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 4 cut(s) 235, 600, 1151, 1533
MvnI CGCG 2 cut(s) 29, 297
MwoI GCNNNNNNNGC 7 cut(s) 174, 717, 801, 1263, 1371, 1438, 2024
NciI CCSGG 1 cut(s) 891
NcoI CCATGG 1 cut(s) 1322
NdeI CATATG 2 cut(s) 138, 402
NdeII GATC 7 cut(s) 151, 751, 774, 865, 1039, 1089, 1215
NheI GCTAGC 1 cut(s) 707
NlaIV GGNNCC 2 cut(s) 673, 1498
NmeAIII GCCGAG 1 cut(s) 2048
NmuCI GTSAC 3 cut(s) 649, 910, 1709
NspI RCATGY 3 cut(s) 214, 1605, 1983
PagI TCATGA 1 cut(s) 577
PciI ACATGT 2 cut(s) 1601, 1979
PcsI WCGNNNNNNNCGW 1 cut(s) 911
PfeI GAWTC 8 cut(s) 13, 370, 474, 821, 1556, 1655, 1872, 1923
PflMI CCANNNNNTGG 3 cut(s) 363, 425, 1898
PkrI GCNGC 7 cut(s) 99, 562, 719, 1407, 1443, 1615, 1825
PleI GAGTC 2 cut(s) 13, 1492
PpsI GAGTC 2 cut(s) 13, 1492
PscI ACATGT 2 cut(s) 1601, 1979
PshAI GACNNNNGTC 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 2029
Psp1406I AACGTT 1 cut(s) 1779
Psp6I CCWGG 4 cut(s) 233, 598, 1149, 1531
PspFI CCCAGC 1 cut(s) 1500
PspGI CCWGG 4 cut(s) 233, 598, 1149, 1531
PspN4I GGNNCC 2 cut(s) 673, 1498
PspPI GGNCC 1 cut(s) 1830
RsaI GTAC 6 cut(s) 182, 673, 972, 1077, 1332, 1459
RsaNI GTAC 6 cut(s) 181, 672, 971, 1076, 1331, 1458
RseI CAYNNNNRTG 1 cut(s) 702
SacI GAGCTC 1 cut(s) 2029
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 8 cut(s) 164, 330, 545, 630, 1221, 1253, 1575, 1818
SatI GCNGC 7 cut(s) 98, 561, 718, 1406, 1442, 1614, 1824
Sau3AI GATC 7 cut(s) 151, 751, 774, 865, 1039, 1089, 1215
Sau96I GGNCC 1 cut(s) 1830
ScaI AGTACT 2 cut(s) 182, 1459
SchI GAGTC 2 cut(s) 13, 1493
ScrFI CCNGG 5 cut(s) 235, 600, 891, 1151, 1533
SduI GDGCHC 3 cut(s) 246, 1501, 2029
SfaNI GCATC 2 cut(s) 109, 1513
SgrDI CGTCGACG 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 702
SmlI CTYRAG 7 cut(s) 163, 271, 629, 964, 1252, 1338, 1360
SmoI CTYRAG 7 cut(s) 163, 271, 629, 964, 1252, 1338, 1360
SsiI CCGC 1 cut(s) 27
SspI AATATT 1 cut(s) 129
SstI GAGCTC 1 cut(s) 2029
StyD4I CCNGG 5 cut(s) 233, 598, 889, 1149, 1531
StyI CCWWGG 2 cut(s) 1322, 1564
TaaI ACNGT 4 cut(s) 528, 724, 1841, 2137
TaiI ACGT 2 cut(s) 948, 1782
TatI WGTACW 4 cut(s) 180, 1075, 1330, 1457
TfiI GAWTC 8 cut(s) 13, 370, 474, 821, 1556, 1655, 1872, 1923
Tru1I TTAA 8 cut(s) 164, 330, 545, 630, 1221, 1253, 1575, 1818
Tru9I TTAA 8 cut(s) 164, 330, 545, 630, 1221, 1253, 1575, 1818
TscAI CASTG 6 cut(s) 223, 531, 658, 1042, 1117, 1243
TseFI GTSAC 3 cut(s) 649, 910, 1709
TseI GCWGC 7 cut(s) 97, 560, 717, 1405, 1441, 1613, 1823
Tsp45I GTSAC 3 cut(s) 649, 910, 1709
TspGWI ACGGA 2 cut(s) 898, 1746
TspRI CASTG 6 cut(s) 223, 531, 658, 1042, 1117, 1243
Van91I CCANNNNNTGG 3 cut(s) 363, 425, 1898
Vha464I CTTAAG 3 cut(s) 163, 629, 1252
XagI CCTNNNNNAGG 1 cut(s) 1379
XapI RAATTY 2 cut(s) 259, 1689
XceI RCATGY 3 cut(s) 214, 1605, 1983
XcmI CCANNNNNNNNNTGG 2 cut(s) 83, 1320
XmiI GTMKAC 2 cut(s) 69, 933
ZraI GACGTC 1 cut(s) 946
ZrmI AGTACT 2 cut(s) 182, 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.