RLG00000023873

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000144
Physical Location & Seq
Forward (+)
29380439 .. 29381734
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023873

Sequence Viewer

Length: 1296 bp
ATGGCAGCGATCAATGGTCAATCCCGTAAGAAAAATAATATGAGCAATGGACAATCCAGTAAGAAACAGAAGAAGATCAATGGGCATTCCCGTAAGAAAACGAGTAAGATCAATGGGCAATCCCGTAGCAGTTGGCAGACACCGCCAGACGATATCATGGAGTACATTCTACAACGTCTGAGTTTATGGGATCGAATACAATTAAGGATGGTGTCCAAGTCTTGGAGGTCAGTAGCTATACGAAGAGATATTCGTAGCTCTCCAACTGAAATGCCCTGGTTACTATTGCCTCCATATGAATCACCAAATCTCAGCAATAACAACTACATAAGCTTTTTAAGCCTTTCTGATGCCAAAGTTCACAAACTGACGCTGCCTAGGGCATTTGGAGGAGGATGGGTTCATGGGTCTTCTAAAGGTTGGTTGGTTATGGTCAAGGGTGCATACTCTAAAATGTTTTTACTAAACCCTATTTCAGGTGCCCAACAGGAACTTCCATCGTTAAAAACTGTCTTTCCATACTTTGCAATTCTCAAAGAGTTGAAAGCAGAGTTGGAGGGCACACATGTCTTGGACAGTTTGTGTAATCAAGTTGTGTTATCTACCTCGGATATTTATTCAGATGATTGTATTGTAGCAGCAATTTTTAGGGATCATATACTGGCTTTGTGCAAACCTGGAGACAAAAGATGGAGGAAAGTCTTGGACTTCAAGGAATCAGATAGGCCGAAGCTTTCTGAAATATTGTTTTCCTGTGGTATGCTATATGCCTTGGTTGATCGTGATGGAAATACCGAGCTTGATATTCCAGCTCGAACCGAAATCTTTGGAGGACACAAGCAGGAAGTAAAGTTCGTCTATGATAGATATCCACCACGTTTACATACTATACATTGGCAATGCCAACCACGCTTGTTAGAATCAACCTCCAAAAATGAAGTCTTGTTGATCCATCAAATCCAAAATATTTGTGGGGAAATAATATCGTTTATAGTATACAAGATGGACGGTGAGACTGAAAACTTCCATGAGGTACAACACTTGGGAGAACAAATTATACTCTTATCAGATTTCGGATCCTCGTCGCTCGCCTCAGCTGCATTGAAAGGGAATCGCATTTATTTTGCAACACATGCTTTTAACAATCAGCAATCAAACCTTGCTATGGGTATATTCTACCTAAGCAGTGGAAGAATTAAGCAATATATTCCTTTTGTCGACAGATCAGTATTGCTACGGCTGGGCAGTTCAGTTGCTAGAGGCCAAGGGACTTGTTGGTTTACTCCGAGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

432

Amino Acids

48.89

Weight (kDa)

9.43

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 49 - 79 2.3e-08 F-box domain
Beta-prop_KIB1-4 PF03478 112 - 385 1.7e-38 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AccB7I CCANNNNNTGG 1 cut(s) 222
AccI GTMKAC 2 cut(s) 996, 1218
AciI CCGC 1 cut(s) 143
AclWI GGATC 5 cut(s) 198, 660, 943, 1071, 1084
AfaI GTAC 2 cut(s) 164, 1035
AfiI CCNNNNNNNGG 3 cut(s) 222, 476, 1165
AflIII ACRYGT 1 cut(s) 565
AgsI TTSAA 3 cut(s) 544, 712, 1105
AjnI CCWGG 2 cut(s) 275, 676
AjuI GAANNNNNNNTTGG 4 cut(s) 477, 509, 536, 568
AloI GAACNNNNNNTCC 4 cut(s) 384, 416, 836, 868
AluBI AGCT 7 cut(s) 236, 258, 333, 733, 799, 812, 1097
AluI AGCT 7 cut(s) 236, 258, 333, 733, 799, 812, 1097
Alw26I GTCTC 2 cut(s) 675, 1007
AlwI GGATC 5 cut(s) 198, 660, 943, 1071, 1084
AoxI GGCC 2 cut(s) 725, 1261
ApeKI GCWGC 4 cut(s) 5, 373, 638, 1097
ArsI GACNNNNNNTTYG 2 cut(s) 924, 956
AspA2I CCTAGG 1 cut(s) 377
AsuHPI GGTGA 2 cut(s) 294, 1022
AvrII CCTAGG 1 cut(s) 377
BaeGI GKGCMC 2 cut(s) 484, 563
BamHI GGATCC 1 cut(s) 1076
BanI GGYRCC 1 cut(s) 479
BbsI GAAGAC 1 cut(s) 402
BbvCI CCTCAGC 1 cut(s) 1093
BbvI GCAGC 4 cut(s) 17, 360, 650, 1084
BccI CCATC 7 cut(s) 202, 390, 505, 684, 779, 960, 997
BceAI ACGGC 1 cut(s) 1253
BciT130I CCWGG 2 cut(s) 277, 678
BcoDI GTCTC 2 cut(s) 675, 1007
BfaI CTAG 2 cut(s) 378, 1257
BisI GCNGC 4 cut(s) 6, 374, 639, 1098
BlnI CCTAGG 1 cut(s) 377
BlsI GCNGC 4 cut(s) 7, 375, 640, 1099
Bme1390I CCNGG 2 cut(s) 277, 678
BmiI GGNNCC 2 cut(s) 481, 1078
BmrFI CCNGG 2 cut(s) 277, 678
BmsI GCATC 1 cut(s) 340
BpiI GAAGAC 1 cut(s) 402
BpmI CTGGAG 1 cut(s) 699
Bpu10I CCTNAGC 2 cut(s) 1093, 1181
BsaBI GATNNNNATC 1 cut(s) 867
BsaJI CCNNGG 5 cut(s) 275, 377, 606, 771, 1264
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 476, 1165
Bse1I ACTGG 2 cut(s) 57, 666
Bse3DI GCAATG 2 cut(s) 52, 905
Bse8I GATNNNNATC 1 cut(s) 867
BseBI CCWGG 2 cut(s) 277, 678
BseDI CCNNGG 5 cut(s) 275, 377, 606, 771, 1264
BseGI GGATG 2 cut(s) 213, 401
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 3 cut(s) 222, 476, 1165
BseMI GCAATG 2 cut(s) 52, 905
BseMII CTCAG 3 cut(s) 170, 325, 1107
BseNI ACTGG 2 cut(s) 57, 666
BseRI GAGGAG 1 cut(s) 405
BseSI GKGCMC 2 cut(s) 484, 563
BseXI GCAGC 4 cut(s) 17, 360, 650, 1084
BseYI CCCAGC 1 cut(s) 1240
BshFI GGCC 2 cut(s) 727, 1263
BshNI GGYRCC 1 cut(s) 479
BslFI GGGAC 1 cut(s) 1282
BslI CCNNNNNNNGG 3 cut(s) 222, 476, 1165
BsmAI GTCTC 2 cut(s) 675, 1007
BsmFI GGGAC 1 cut(s) 1282
BsmI GAATGC 1 cut(s) 85
BsnI GGCC 2 cut(s) 727, 1263
Bsp1286I GDGCHC 2 cut(s) 484, 563
Bsp143I GATC 9 cut(s) 9, 75, 108, 190, 652, 778, 948, 1076, 1223
BspACI CCGC 1 cut(s) 143
BspANI GGCC 2 cut(s) 727, 1263
BspCNI CTCAG 3 cut(s) 171, 324, 1106
BspLI GGNNCC 2 cut(s) 481, 1078
BspPI GGATC 5 cut(s) 198, 660, 943, 1071, 1084
BspT107I GGYRCC 1 cut(s) 479
BsrDI GCAATG 2 cut(s) 52, 905
BsrI ACTGG 2 cut(s) 57, 666
BssECI CCNNGG 5 cut(s) 275, 377, 606, 771, 1264
BssMI GATC 9 cut(s) 9, 75, 108, 190, 652, 778, 948, 1076, 1223
BssNAI GTATAC 1 cut(s) 997
BssT1I CCWWGG 3 cut(s) 377, 771, 1264
Bst1107I GTATAC 1 cut(s) 997
Bst2UI CCWGG 2 cut(s) 277, 678
Bst4CI ACNGT 3 cut(s) 511, 578, 1010
Bst6I CTCTTC 1 cut(s) 238
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstC8I GCNNGC 1 cut(s) 1089
BstDEI CTNAG 4 cut(s) 179, 311, 1093, 1181
BstENI CCTNNNNNAGG 1 cut(s) 474
BstF5I GGATG 2 cut(s) 213, 401
BstKTI GATC 9 cut(s) 12, 78, 111, 193, 655, 781, 951, 1079, 1226
BstMAI GTCTC 2 cut(s) 675, 1007
BstMBI GATC 9 cut(s) 9, 75, 108, 190, 652, 778, 948, 1076, 1223
BstMWI GCNNNNNNNGC 5 cut(s) 142, 339, 909, 1097, 1133
BstNI CCWGG 2 cut(s) 277, 678
BstNSI RCATGY 2 cut(s) 569, 1136
BstSCI CCNGG 2 cut(s) 275, 676
BstSLI GKGCMC 2 cut(s) 484, 563
BstV1I GCAGC 4 cut(s) 17, 360, 650, 1084
BstV2I GAAGAC 1 cut(s) 402
BstX2I RGATCY 1 cut(s) 1076
BstYI RGATCY 1 cut(s) 1076
BstZ17I GTATAC 1 cut(s) 997
BsuRI GGCC 2 cut(s) 727, 1263
BtsCI GGATG 2 cut(s) 213, 401
BtsI GCAGTG 1 cut(s) 1192
BtsIMutI CAGTG 1 cut(s) 1192
Cac8I GCNNGC 1 cut(s) 1089
CseI GACGC 1 cut(s) 379
Csp6I GTAC 2 cut(s) 163, 1034
CviAII CATG 5 cut(s) 157, 404, 566, 1028, 1133
CviQI GTAC 2 cut(s) 163, 1034
DdeI CTNAG 4 cut(s) 179, 311, 1093, 1181
DpnI GATC 9 cut(s) 11, 77, 110, 192, 654, 780, 950, 1078, 1225
DpnII GATC 9 cut(s) 9, 75, 108, 190, 652, 778, 948, 1076, 1223
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
Eco130I CCWWGG 3 cut(s) 377, 771, 1264
Eco32I GATATC 2 cut(s) 154, 869
EcoNI CCTNNNNNAGG 1 cut(s) 474
EcoRII CCWGG 2 cut(s) 275, 676
EcoRV GATATC 2 cut(s) 154, 869
EcoT14I CCWWGG 3 cut(s) 377, 771, 1264
ErhI CCWWGG 3 cut(s) 377, 771, 1264
FaeI CATG 5 cut(s) 160, 407, 569, 1031, 1136
FaqI GGGAC 1 cut(s) 1282
FatI CATG 5 cut(s) 156, 403, 565, 1027, 1132
FauNDI CATATG 1 cut(s) 295
FblI GTMKAC 2 cut(s) 996, 1218
Fnu4HI GCNGC 4 cut(s) 6, 374, 639, 1098
FokI GGATG 2 cut(s) 220, 408
Fsp4HI GCNGC 4 cut(s) 6, 374, 639, 1098
FspBI CTAG 2 cut(s) 378, 1257
GluI GCNGC 4 cut(s) 6, 374, 639, 1098
GsaI CCCAGC 1 cut(s) 1244
GsuI CTGGAG 1 cut(s) 699
HaeIII GGCC 2 cut(s) 727, 1263
HgaI GACGC 1 cut(s) 379
Hin1II CATG 5 cut(s) 160, 407, 569, 1031, 1136
HincII GTYRAC 1 cut(s) 1219
HindII GTYRAC 1 cut(s) 1219
HindIII AAGCTT 2 cut(s) 331, 731
HinfI GANTC 4 cut(s) 299, 716, 920, 1111
HphI GGTGA 2 cut(s) 294, 1022
Hpy166II GTNNAC 5 cut(s) 361, 881, 997, 1219, 1281
Hpy188I TCNGA 9 cut(s) 180, 349, 610, 622, 721, 739, 1069, 1076, 1287
Hpy188III TCNNGA 1 cut(s) 782
Hpy8I GTNNAC 5 cut(s) 361, 881, 997, 1219, 1281
Hpy99I CGWCG 1 cut(s) 1087
HpyCH4III ACNGT 3 cut(s) 511, 578, 1010
HpyCH4IV ACGT 2 cut(s) 175, 877
HpyCH4V TGCA 5 cut(s) 443, 527, 672, 1100, 1127
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 339, 909, 1097, 1133
HpyF3I CTNAG 4 cut(s) 179, 311, 1093, 1181
HpySE526I ACGT 2 cut(s) 175, 877
Hsp92II CATG 5 cut(s) 160, 407, 569, 1031, 1136
Kzo9I GATC 9 cut(s) 9, 75, 108, 190, 652, 778, 948, 1076, 1223
Lsp1109I GCAGC 4 cut(s) 17, 360, 650, 1084
LweI GCATC 1 cut(s) 340
MaeI CTAG 2 cut(s) 378, 1257
MaeII ACGT 2 cut(s) 175, 877
MaeIII GTNAC 1 cut(s) 279
MalI GATC 9 cut(s) 11, 77, 110, 192, 654, 780, 950, 1078, 1225
MboI GATC 9 cut(s) 9, 75, 108, 190, 652, 778, 948, 1076, 1223
MboII GAAGA 5 cut(s) 82, 85, 255, 402, 1203
MflI RGATCY 1 cut(s) 1076
MhlI GDGCHC 2 cut(s) 484, 563
MluCI AATT 5 cut(s) 200, 528, 642, 1053, 1194
MmeI TCCRAC 2 cut(s) 287, 534
MseI TTAA 5 cut(s) 203, 338, 503, 1140, 1197
MspA1I CMGCKG 1 cut(s) 1097
MspR9I CCNGG 2 cut(s) 277, 678
Mva1269I GAATGC 1 cut(s) 85
MvaI CCWGG 2 cut(s) 277, 678
MwoI GCNNNNNNNGC 5 cut(s) 142, 339, 909, 1097, 1133
NdeI CATATG 1 cut(s) 295
NdeII GATC 9 cut(s) 9, 75, 108, 190, 652, 778, 948, 1076, 1223
NlaIII CATG 5 cut(s) 160, 407, 569, 1031, 1136
NlaIV GGNNCC 2 cut(s) 481, 1078
NspI RCATGY 2 cut(s) 569, 1136
PciI ACATGT 1 cut(s) 565
PctI GAATGC 1 cut(s) 85
PfeI GAWTC 4 cut(s) 299, 716, 920, 1111
PflMI CCANNNNNTGG 1 cut(s) 222
PkrI GCNGC 4 cut(s) 7, 375, 640, 1099
PscI ACATGT 1 cut(s) 565
Psp6I CCWGG 2 cut(s) 275, 676
PspFI CCCAGC 1 cut(s) 1240
PspGI CCWGG 2 cut(s) 275, 676
PspN4I GGNNCC 2 cut(s) 481, 1078
PsrI GAACNNNNNNTAC 2 cut(s) 1041, 1073
PsuI RGATCY 1 cut(s) 1076
PvuII CAGCTG 1 cut(s) 1097
RsaI GTAC 2 cut(s) 164, 1035
RsaNI GTAC 2 cut(s) 163, 1034
SalI GTCGAC 1 cut(s) 1217
SaqAI TTAA 5 cut(s) 203, 338, 503, 1140, 1197
SatI GCNGC 4 cut(s) 6, 374, 639, 1098
Sau3AI GATC 9 cut(s) 9, 75, 108, 190, 652, 778, 948, 1076, 1223
ScrFI CCNGG 2 cut(s) 277, 678
SduI GDGCHC 2 cut(s) 484, 563
SfaNI GCATC 1 cut(s) 340
Sse9I AATT 5 cut(s) 200, 528, 642, 1053, 1194
SsiI CCGC 1 cut(s) 143
SspI AATATT 2 cut(s) 744, 967
SspMI CTAG 2 cut(s) 378, 1257
StyD4I CCNGG 2 cut(s) 275, 676
StyI CCWWGG 3 cut(s) 377, 771, 1264
TaaI ACNGT 3 cut(s) 511, 578, 1010
TaiI ACGT 2 cut(s) 178, 880
TaqI TCGA 3 cut(s) 193, 814, 1218
TasI AATT 5 cut(s) 200, 528, 642, 1053, 1194
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 4 cut(s) 299, 716, 920, 1111
Tru1I TTAA 5 cut(s) 203, 338, 503, 1140, 1197
Tru9I TTAA 5 cut(s) 203, 338, 503, 1140, 1197
TscAI CASTG 1 cut(s) 1192
TseI GCWGC 4 cut(s) 5, 373, 638, 1097
TspDTI ATGAA 3 cut(s) 312, 392, 951
TspRI CASTG 1 cut(s) 1192
Van91I CCANNNNNTGG 1 cut(s) 222
XagI CCTNNNNNAGG 1 cut(s) 474
XceI RCATGY 2 cut(s) 569, 1136
XcmI CCANNNNNNNNNTGG 1 cut(s) 968
XmaJI CCTAGG 1 cut(s) 377
XmiI GTMKAC 2 cut(s) 996, 1218
XspI CTAG 2 cut(s) 378, 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.