Rh7CG213700

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000144
Physical Location & Seq
Reverse (-)
19328616 .. 19330185
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG213700.1

Sequence Viewer

Length: 1542 bp
ATGAGCAGCAACCCAACTCGTTTCCGGAGTTGGCAAGAGCCACCACCAGATATTATGGAGAAGATTATACAGCGTCTCGACATAACCGATCGAATACGTTTAAGATTTGTCTGTGAGTCTTGGAGGTCAGTTGCTAGGCGAAGAGACATCCGTGGTGCTCCGCAGCTCCCCTGGTTAGTACACCCTCATAACCCTAGGTCCAAGTACTTGATATTTTCGAGCCTCACTAATGGTCAAGTTCACAAGTTGAGGCTCCCTCCAGGAGGGTGGTGGTTTTATGGTTCTTCTAAAGGTTGGTTGATTATGGTCAAAGGAAAAGGACTAAACGCTGAGATACTTGTACTAAACCCGATTTCTGGAGCCCAGCACAAGGTTCCATCTTTCCATGATGAGTCTGTAGTAATTGATCATGAAGCTGATTCTTTCCTTGAAAATATTGAATTATCTAGCGCAAACGAGTCCGAATGTATGGTGGCTGCAGTGTTTAAGTGTGTATTAGGTTTGTGCAAACTTGGAGACAAAAGCTGGACATTCTTCCTGATATCAGGGTATATTATAAATATACTGTTTTCATGTGGCAAATTATATGCCGTGCTCCATGGTGATGACGGTAACATGGAAGATGAGAGTTTGAATTTTGGAGATAATGTAGTAGAAGTGAAGTTCGTCTTTGACAGTTTTGAAAATTTATTGAATAACCCCGAGTTTATTGTACCAGAGATTTATCCCGACTCTACGATCTTGCGGAATAGTGAAGTGAACTCATATTTGTTAGAATCAGCGAACAATGAAGTTTTGTTGATCCATCAAATTTATGACCGAAAAACAATGTATGTGGGTGAGGAAGATGATCTAATTCACATCTGCATTATCATCCACATCATCAGCATTCTGTTATGGCAGATCGGCCTTATGATTGAACTCAATTCTTTTACTGTTGCAGATGCCCTTGTATGCTTATTCATTTTCGTTGGTGCTTTCCTCGTTTCCTTTTTTCTTGGTGGTGTTTTCGTCGCCGGTAATAAGGATGATGAGGGTGGTGATGAAGGAAACAACAACAATGAACAATTAAAAGGAGATGACGACGATGAAGCGGACGGTGCACCAGTAGGTGACGACGACAATGAGGATGGTGAGAGTGATGAGGAAGTTGACGTCAGCGACGAAGAAGATGAGGGCGGTGTGGAAGGTGATAGAGATCTAGGTTACGAAGGACAGAATGTGTACTTTAGAACAAGTGGTTTTAGGGTATACAAGATTGGCTCGGGCAACAACTTTGATAGGGTACAAAGTTTGGGGGATCAAATATTATTATTGGCAGACCAAGGTTCTTCCTTGTGCCTCTCAGCAAGTGACTTCAAAGGCTTAGATGGGAATTGCATTTATTTTGCGCCAAACGGAATGCGTAGGCTACATCCTTTGGAAACACTCAAACCAAATATGAATTATGATCTCAGTGTCTTCTGCTTAGATGATGAAAAAATTGAGCCATTTTTTCCAGGTTTTGACATGCAAATGCGTTTCTTCATTCCATGTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

513

Amino Acids

57.75

Weight (kDa)

4.46

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 15 - 49 6.4e-09 F-box domain
Beta-prop_KIB1-4 PF03478 73 - 273 4.4e-15 KIB1-4 beta-propeller
Beta-prop_KIB1-4 PF03478 410 - 466 3.4e-12 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 557, 1540
AatII GACGTC 1 cut(s) 1158
AccI GTMKAC 1 cut(s) 1251
AccIII TCCGGA 1 cut(s) 24
AciI CCGC 4 cut(s) 161, 745, 1094, 1179
AclWI GGATC 2 cut(s) 796, 1308
AcsI RAATTY 3 cut(s) 634, 685, 810
AcyI GRCGYC 1 cut(s) 1155
AfaI GTAC 6 cut(s) 180, 206, 342, 714, 1226, 1287
AfiI CCNNNNNNNGG 3 cut(s) 263, 356, 370
AgsI TTSAA 7 cut(s) 431, 440, 634, 683, 694, 920, 1360
AjnI CCWGG 3 cut(s) 170, 259, 1498
AluBI AGCT 3 cut(s) 166, 416, 525
AluI AGCT 3 cut(s) 166, 416, 525
Alw21I GWGCWC 3 cut(s) 160, 597, 1105
Alw26I GTCTC 3 cut(s) 80, 138, 510
Alw44I GTGCAC 1 cut(s) 1101
AlwI GGATC 2 cut(s) 796, 1308
Ama87I CYCGRG 2 cut(s) 701, 1264
Aor13HI TCCGGA 1 cut(s) 24
AoxI GGCC 1 cut(s) 907
ApaLI GTGCAC 1 cut(s) 1101
ApeKI GCWGC 3 cut(s) 6, 163, 476
ApoI RAATTY 3 cut(s) 634, 685, 810
AspA2I CCTAGG 1 cut(s) 194
AspLEI GCGC 2 cut(s) 452, 1393
AspS9I GGNCC 1 cut(s) 198
AsuHPI GGTGA 6 cut(s) 614, 851, 1052, 1124, 1145, 1202
AvaI CYCGRG 2 cut(s) 701, 1264
AvaII GGWCC 1 cut(s) 198
AvrII CCTAGG 1 cut(s) 194
BaeGI GKGCMC 1 cut(s) 1105
BanII GRGCYC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 1453
Bbv12I GWGCWC 3 cut(s) 160, 597, 1105
BbvI GCAGC 3 cut(s) 18, 175, 463
BccI CCATC 4 cut(s) 385, 813, 1124, 1364
BceAI ACGGC 1 cut(s) 575
BciT130I CCWGG 3 cut(s) 172, 261, 1500
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 3 cut(s) 80, 138, 510
BfaI CTAG 4 cut(s) 135, 195, 447, 1202
BfmI CTRYAG 2 cut(s) 396, 477
BglII AGATCT 1 cut(s) 1198
BisI GCNGC 3 cut(s) 7, 164, 477
BlnI CCTAGG 1 cut(s) 194
BlsI GCNGC 3 cut(s) 8, 165, 478
BmcAI AGTACT 1 cut(s) 206
Bme1390I CCNGG 3 cut(s) 172, 261, 1500
Bme18I GGWCC 1 cut(s) 198
BmeT110I CYCGRG 2 cut(s) 701, 1264
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 3 cut(s) 254, 361, 375
BmrFI CCNGG 3 cut(s) 172, 261, 1500
BmsI GCATC 1 cut(s) 934
BpiI GAAGAC 1 cut(s) 1453
BplI GAGNNNNNCTC 2 cut(s) 241, 273
BpmI CTGGAG 2 cut(s) 243, 378
BsaBI GATNNNNATC 1 cut(s) 1197
BsaHI GRCGYC 1 cut(s) 1155
BsaJI CCNNGG 5 cut(s) 151, 170, 194, 598, 1324
BsaWI WCCGGW 1 cut(s) 24
BsaXI ACNNNNNCTCC 2 cut(s) 633, 663
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 356, 370
Bse118I RCCGGY 1 cut(s) 1016
Bse1I ACTGG 1 cut(s) 1106
Bse8I GATNNNNATC 1 cut(s) 1197
BseAI TCCGGA 1 cut(s) 24
BseBI CCWGG 3 cut(s) 172, 261, 1500
BseDI CCNNGG 5 cut(s) 151, 170, 194, 598, 1324
BseGI GGATG 5 cut(s) 147, 873, 1033, 1135, 1414
BseJI GATNNNNATC 1 cut(s) 1197
BseLI CCNNNNNNNGG 3 cut(s) 263, 356, 370
BseMII CTCAG 3 cut(s) 321, 1359, 1468
BseNI ACTGG 1 cut(s) 1106
BseSI GKGCMC 1 cut(s) 1105
BseXI GCAGC 3 cut(s) 18, 175, 463
BseYI CCCAGC 1 cut(s) 363
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 1 cut(s) 909
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 3 cut(s) 160, 597, 1105
BsiHKCI CYCGRG 2 cut(s) 701, 1264
BsiSI CCGG 2 cut(s) 25, 1017
BslI CCNNNNNNNGG 3 cut(s) 263, 356, 370
BsmAI GTCTC 3 cut(s) 80, 138, 510
BsmBI CGTCTC 1 cut(s) 80
BsmI GAATGC 2 cut(s) 888, 1407
BsnI GGCC 1 cut(s) 909
BsoBI CYCGRG 2 cut(s) 701, 1264
Bsp1286I GDGCHC 4 cut(s) 160, 364, 597, 1105
Bsp13I TCCGGA 1 cut(s) 24
Bsp143I GATC 9 cut(s) 88, 406, 738, 801, 850, 903, 1198, 1300, 1450
Bsp19I CCATGG 1 cut(s) 598
BspACI CCGC 4 cut(s) 161, 745, 1094, 1179
BspANI GGCC 1 cut(s) 909
BspCNI CTCAG 3 cut(s) 322, 1358, 1467
BspEI TCCGGA 1 cut(s) 24
BspHI TCATGA 1 cut(s) 409
BspLI GGNNCC 3 cut(s) 254, 361, 375
BspMAI CTGCAG 1 cut(s) 481
BspPI GGATC 2 cut(s) 796, 1308
BsrFI RCCGGY 1 cut(s) 1016
BsrI ACTGG 1 cut(s) 1106
BssAI RCCGGY 1 cut(s) 1016
BssECI CCNNGG 5 cut(s) 151, 170, 194, 598, 1324
BssMI GATC 9 cut(s) 88, 406, 738, 801, 850, 903, 1198, 1300, 1450
BssNAI GTATAC 1 cut(s) 1252
BssNI GRCGYC 1 cut(s) 1155
BssT1I CCWWGG 3 cut(s) 194, 598, 1324
Bst1107I GTATAC 1 cut(s) 1252
Bst2UI CCWGG 3 cut(s) 172, 261, 1500
Bst4CI ACNGT 5 cut(s) 567, 611, 677, 937, 1100
Bst6I CTCTTC 1 cut(s) 136
BstACI GRCGYC 1 cut(s) 1155
BstDEI CTNAG 5 cut(s) 330, 1345, 1366, 1454, 1468
BstDSI CCRYGG 2 cut(s) 151, 598
BstENI CCTNNNNNAGG 1 cut(s) 261
BstF5I GGATG 5 cut(s) 147, 873, 1033, 1135, 1414
BstHHI GCGC 2 cut(s) 452, 1393
BstKTI GATC 9 cut(s) 91, 409, 741, 804, 853, 906, 1201, 1303, 1453
BstMAI GTCTC 3 cut(s) 80, 138, 510
BstMBI GATC 9 cut(s) 88, 406, 738, 801, 850, 903, 1198, 1300, 1450
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 1 cut(s) 1100
BstNI CCWGG 3 cut(s) 172, 261, 1500
BstNSI RCATGY 1 cut(s) 1513
BstSCI CCNGG 3 cut(s) 170, 259, 1498
BstSFI CTRYAG 2 cut(s) 396, 477
BstSLI GKGCMC 1 cut(s) 1105
BstV1I GCAGC 3 cut(s) 18, 175, 463
BstV2I GAAGAC 1 cut(s) 1453
BstX2I RGATCY 1 cut(s) 1198
BstXI CCANNNNNNTGG 1 cut(s) 267
BstYI RGATCY 1 cut(s) 1198
BstZ17I GTATAC 1 cut(s) 1252
BsuRI GGCC 1 cut(s) 909
BtgI CCRYGG 2 cut(s) 151, 598
BtsCI GGATG 5 cut(s) 147, 873, 1033, 1135, 1414
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 486, 1462
CciI TCATGA 1 cut(s) 409
CfoI GCGC 2 cut(s) 452, 1393
Cfr10I RCCGGY 1 cut(s) 1016
Cfr13I GGNCC 1 cut(s) 198
CseI GACGC 1 cut(s) 62
Csp6I GTAC 6 cut(s) 179, 205, 341, 713, 1225, 1286
CspCI CAANNNNNGTGG 2 cut(s) 816, 851
CviAII CATG 7 cut(s) 386, 410, 573, 599, 616, 1510, 1533
CviQI GTAC 6 cut(s) 179, 205, 341, 713, 1225, 1286
DdeI CTNAG 5 cut(s) 330, 1345, 1366, 1454, 1468
DpnI GATC 9 cut(s) 90, 408, 740, 803, 852, 905, 1200, 1302, 1452
DpnII GATC 9 cut(s) 88, 406, 738, 801, 850, 903, 1198, 1300, 1450
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
Eco130I CCWWGG 3 cut(s) 194, 598, 1324
Eco24I GRGCYC 1 cut(s) 364
Eco32I GATATC 1 cut(s) 543
Eco47I GGWCC 1 cut(s) 198
Eco88I CYCGRG 2 cut(s) 701, 1264
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoRII CCWGG 3 cut(s) 170, 259, 1498
EcoRV GATATC 1 cut(s) 543
EcoT14I CCWWGG 3 cut(s) 194, 598, 1324
EcoT38I GRGCYC 1 cut(s) 364
ErhI CCWWGG 3 cut(s) 194, 598, 1324
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 7 cut(s) 389, 413, 576, 602, 619, 1513, 1536
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FatI CATG 7 cut(s) 385, 409, 572, 598, 615, 1509, 1532
FbaI TGATCA 1 cut(s) 406
FblI GTMKAC 1 cut(s) 1251
Fnu4HI GCNGC 3 cut(s) 7, 164, 477
FokI GGATG 5 cut(s) 134, 860, 1040, 1142, 1401
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 3 cut(s) 7, 164, 477
FspBI CTAG 4 cut(s) 135, 195, 447, 1202
GlaI GCGC 2 cut(s) 451, 1392
GluI GCNGC 3 cut(s) 7, 164, 477
GsaI CCCAGC 1 cut(s) 367
GsuI CTGGAG 2 cut(s) 243, 378
HaeIII GGCC 1 cut(s) 909
HapII CCGG 2 cut(s) 25, 1017
HgaI GACGC 1 cut(s) 62
HhaI GCGC 2 cut(s) 452, 1393
Hin1I GRCGYC 1 cut(s) 1155
Hin1II CATG 7 cut(s) 389, 413, 576, 602, 619, 1513, 1536
Hin6I GCGC 2 cut(s) 450, 1391
HinP1I GCGC 2 cut(s) 450, 1391
HincII GTYRAC 1 cut(s) 1153
HindII GTYRAC 1 cut(s) 1153
HinfI GANTC 6 cut(s) 116, 392, 419, 458, 731, 776
HpaII CCGG 2 cut(s) 25, 1017
HphI GGTGA 6 cut(s) 614, 851, 1052, 1124, 1145, 1202
Hpy166II GTNNAC 7 cut(s) 181, 241, 760, 1103, 1153, 1225, 1252
Hpy188I TCNGA 1 cut(s) 463
Hpy188III TCNNGA 6 cut(s) 25, 77, 357, 410, 538, 728
Hpy8I GTNNAC 7 cut(s) 181, 241, 760, 1103, 1153, 1225, 1252
Hpy99I CGWCG 4 cut(s) 1016, 1088, 1121, 1166
HpyAV CCTTC 3 cut(s) 1040, 1181, 1205
HpyCH4III ACNGT 5 cut(s) 567, 611, 677, 937, 1100
HpyCH4IV ACGT 2 cut(s) 97, 1155
HpyCH4V TGCA 7 cut(s) 479, 507, 867, 941, 1103, 1380, 1513
HpyF10VI GCNNNNNNNGC 1 cut(s) 1100
HpyF3I CTNAG 5 cut(s) 330, 1345, 1366, 1454, 1468
HpySE526I ACGT 2 cut(s) 97, 1155
Hsp92I GRCGYC 1 cut(s) 1155
Hsp92II CATG 7 cut(s) 389, 413, 576, 602, 619, 1513, 1536
HspAI GCGC 2 cut(s) 450, 1391
Kpn2I TCCGGA 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 9 cut(s) 88, 406, 738, 801, 850, 903, 1198, 1300, 1450
LmnI GCTCC 5 cut(s) 163, 171, 258, 359, 600
Lsp1109I GCAGC 3 cut(s) 18, 175, 463
LweI GCATC 1 cut(s) 934
MaeI CTAG 4 cut(s) 135, 195, 447, 1202
MaeII ACGT 2 cut(s) 97, 1155
MaeIII GTNAC 4 cut(s) 611, 1112, 1205, 1352
MalI GATC 9 cut(s) 90, 408, 740, 803, 852, 905, 1200, 1302, 1452
MboI GATC 9 cut(s) 88, 406, 738, 801, 850, 903, 1198, 1300, 1450
MflI RGATCY 1 cut(s) 1198
MhlI GDGCHC 4 cut(s) 160, 364, 597, 1105
MlyI GAGTC 4 cut(s) 125, 401, 467, 725
MroI TCCGGA 1 cut(s) 24
MseI TTAA 3 cut(s) 101, 486, 1070
MslI CAYNNNNRTG 2 cut(s) 603, 1514
MspI CCGG 2 cut(s) 25, 1017
MspR9I CCNGG 3 cut(s) 172, 261, 1500
Mva1269I GAATGC 2 cut(s) 888, 1407
MvaI CCWGG 3 cut(s) 172, 261, 1500
MwoI GCNNNNNNNGC 1 cut(s) 1100
NcoI CCATGG 1 cut(s) 598
NdeII GATC 9 cut(s) 88, 406, 738, 801, 850, 903, 1198, 1300, 1450
NlaIII CATG 7 cut(s) 389, 413, 576, 602, 619, 1513, 1536
NlaIV GGNNCC 3 cut(s) 254, 361, 375
NmuCI GTSAC 2 cut(s) 1112, 1352
NspI RCATGY 1 cut(s) 1513
PagI TCATGA 1 cut(s) 409
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 1161
PctI GAATGC 2 cut(s) 888, 1407
PfeI GAWTC 2 cut(s) 419, 776
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 3 cut(s) 8, 165, 478
Ple19I CGATCG 1 cut(s) 91
PleI GAGTC 4 cut(s) 124, 400, 466, 725
PpsI GAGTC 4 cut(s) 124, 400, 466, 725
PsiI TTATAA 2 cut(s) 557, 1540
Psp6I CCWGG 3 cut(s) 170, 259, 1498
PspFI CCCAGC 1 cut(s) 363
PspGI CCWGG 3 cut(s) 170, 259, 1498
PspN4I GGNNCC 3 cut(s) 254, 361, 375
PspPI GGNCC 1 cut(s) 198
PstI CTGCAG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 1198
PvuI CGATCG 1 cut(s) 91
RsaI GTAC 6 cut(s) 180, 206, 342, 714, 1226, 1287
RsaNI GTAC 6 cut(s) 179, 205, 341, 713, 1225, 1286
RseI CAYNNNNRTG 2 cut(s) 603, 1514
SaqAI TTAA 3 cut(s) 101, 486, 1070
SatI GCNGC 3 cut(s) 7, 164, 477
Sau3AI GATC 9 cut(s) 88, 406, 738, 801, 850, 903, 1198, 1300, 1450
Sau96I GGNCC 1 cut(s) 198
ScaI AGTACT 1 cut(s) 206
SchI GAGTC 4 cut(s) 125, 401, 467, 725
ScrFI CCNGG 3 cut(s) 172, 261, 1500
SduI GDGCHC 4 cut(s) 160, 364, 597, 1105
SfaNI GCATC 1 cut(s) 934
SfcI CTRYAG 2 cut(s) 396, 477
SinI GGWCC 1 cut(s) 198
SmiMI CAYNNNNRTG 2 cut(s) 603, 1514
SsiI CCGC 4 cut(s) 161, 745, 1094, 1179
SspI AATATT 2 cut(s) 436, 1308
SspMI CTAG 4 cut(s) 135, 195, 447, 1202
StyD4I CCNGG 3 cut(s) 170, 259, 1498
StyI CCWWGG 3 cut(s) 194, 598, 1324
TaaI ACNGT 5 cut(s) 567, 611, 677, 937, 1100
TaiI ACGT 2 cut(s) 100, 1158
TaqI TCGA 3 cut(s) 78, 91, 218
TaqII GACCGA 1 cut(s) 834
TatI WGTACW 4 cut(s) 178, 204, 340, 1224
TfiI GAWTC 2 cut(s) 419, 776
Tru1I TTAA 3 cut(s) 101, 486, 1070
Tru9I TTAA 3 cut(s) 101, 486, 1070
TscAI CASTG 2 cut(s) 486, 1462
TseFI GTSAC 2 cut(s) 1112, 1352
TseI GCWGC 3 cut(s) 6, 163, 476
Tsp45I GTSAC 2 cut(s) 1112, 1352
TspGWI ACGGA 2 cut(s) 140, 1413
TspRI CASTG 2 cut(s) 486, 1462
VneI GTGCAC 1 cut(s) 1101
VpaK11BI GGWCC 1 cut(s) 198
XagI CCTNNNNNAGG 1 cut(s) 261
XapI RAATTY 3 cut(s) 634, 685, 810
XceI RCATGY 1 cut(s) 1513
XcmI CCANNNNNNNNNTGG 1 cut(s) 267
XmaJI CCTAGG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 1251
XspI CTAG 4 cut(s) 135, 195, 447, 1202
ZraI GACGTC 1 cut(s) 1156
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.