FvH4_2g16550

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000144
Physical Location & Seq
Reverse (-)
14424183 .. 14427929
3747 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g16550.t1

Sequence Viewer

Length: 2055 bp
ATGGCAACACTAGCACATGATGCAACTGCAATGCTTGCAGCAACCACAAGTGCGCCACAATTCTGGCATGCAGTTACAGGAGCAACAAATCACATGTCCTGCAATCCCATGATGCTCAACAATGTCACCCCTTACAATGCCTCAGACTTAGTTCAGAATCTTCATATTAGTAATGCCGTAATGGAGCACAACTGCCTATATATCACTCATACCGGACAAGGTGTTGGGCACCCAATTCTCTACAAGGGACTAAGTAAGCAAGGGTTGTATCCCATTCTTTTGATCTACCACTGCTCAATAAGTCTGCAGAGAAATCTACAACAGCTGTGGCTTCCTCTCCTTCAACTTAAGCTGCTAGTTTTGTTGGAAAACTTGTCAAGTATTCACTATGTCATCAAAGGTTTGGCTTATGCAGGTCATGAGCAATTCTCAGACTTGGCAATGGTTGTGGTGGACCTTATGGTATACTTCTCGCTGCTGCTGGCGGCCGGCGATGGCAACGACAATGTTAGCATGCATGGGTGGGGTGTGTGTACCGATTGGAAAGGCAATGAGGTTGATGGTGAAGGATTGAAGGTGTTTATGACGGAGAAGGCCGGCATTGAGGATGGTTTATGGGGACCAAGTTGTGTAGCGTACAAAATAGATAACGAGTGCGGTAACTTTGTTGAAGTATTTGTACGGAGAGACGAAAGACAAGACAGCAAGATAAAGGAAAATGAGGAGTTGAGTTGCTGGCAGGAACCACCTGAAGATGTCAAAGAGATGATTGTTAAGCGCATGAGTTTGAGTGGTCGAGCTCGCTTAAGCAGTTTGTGTAATTCTTGGAGGTTAGTTTGTATGCGAAGAGAAATCCCGACTTGTCCACAACTCCCATGGTTAGTACACCCTCAACCCAAAACAAGAGATAGCTTGACCTTCTCAAGCCTCTCAGAGGGCAAAGTTTTCAAATCGAGGCTACCTTGCCCTGATTTCAAGTCATCGAGGCTGAATAAACTAGTTGGGAGAATTACTAATCAGAGATCAGCTTATTTCCTCGATTCTTCCAGAGGTTGGTTGGTTATGCTGGACTGGGAGCACTATAACAGGATAACCAGAAGACGAAAGCGAGCAATATATCTTGTAAATCCAGTTTTAGGAGCACAGCAAAGACTTCCTCCAGTTCCCGGCTATAAAGAAAATTTTGACAATTCTCTCATTAATCCTAATGTTGTATTATCTAGCCCAGATGTATCAACATGTTTGGTCGCAGCAACTCTTGGTACTCAAAAACTGCTTTTATGCAGACCTAGAGACAAAAGCTGGACCGTCTTTCAGGTATTTGATGAAAACGACAGTATTGGCTGTGTTTACAATATATTGTTTTCCAGTACAGGGACGACTCTATATGCCTTGGTTAGGGATAGCAGTGAATGTGGTGCTGAAACCTGGATTGAAAATCGCAGTTTAAAGTCCGGAGATGAAGAAGTGAAATTGAAGGTGATATATGAGAAAGAAGAATGGTGGATAGATGAAGGGTTACTAATCCAAGAATTCGAAGGAGAGATGTGCACGACATACTTGTCAGAAACAACAAACAATCAATTGCTGTTGATTTATCAAATTCAAGGCAATGGTGGTGAAGATGTCATGGAAGGCCATGTGGTTGATGAGGAAGGCAACATCAACAACAACAACTTCGGAGAAGGAGATGAGAATGACATGGAAGGTAATGAGCAGAACGAGAACGTTCTAGGGTGGGTTGGACAATTCTTATGTAAAGCACTGCACCTGCTGAAATCTTCTTTTAGTGATGATAATGTCTATCTGAGGACAAGATGTTTTATAGTATACAAGCTTGATCCAGACAATAATAAGTTTCGTAGGGTACAAGATTTAGGAGACCAAATATTATTTCTGGCAGAGGATAATTCTTCATTGTCCCTTTTAGCCAGCGATTTCAAAGACTTACGAGGGAATTGCATCTATTTCGCAACAAGAGCAAAGGATTATGAAACTATTCACACATCACGTGAGATCGGCATTTTCTACTTAGATACTGGAAGAATTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

685

Amino Acids

77.51

Weight (kDa)

5.27

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 322 - 676 1.7e-31 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1779
AasI GACNNNNNNGTC 1 cut(s) 1561
Acc36I ACCTGC 2 cut(s) 404, 1779
AccB1I GGYRCC 1 cut(s) 228
AccB7I CCANNNNNTGG 1 cut(s) 1053
AccI GTMKAC 2 cut(s) 465, 1830
AccIII TCCGGA 1 cut(s) 1454
AciI CCGC 2 cut(s) 485, 657
AclI AACGTT 1 cut(s) 1728
AclWI GGATC 1 cut(s) 1835
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 3 cut(s) 1180, 1532, 1602
AcuI CTGAAG 1 cut(s) 771
AcvI CACGTG 1 cut(s) 2012
AfaI GTAC 7 cut(s) 535, 638, 681, 885, 1264, 1372, 1869
AfiI CCNNNNNNNGG 6 cut(s) 934, 1053, 1136, 1166, 1374, 1398
AflII CTTAAG 2 cut(s) 347, 805
AflIII ACRYGT 2 cut(s) 93, 1238
AgsI TTSAA 9 cut(s) 344, 574, 671, 949, 976, 1436, 1477, 1607, 1942
AhlI ACTAGT 1 cut(s) 997
AjnI CCWGG 1 cut(s) 1427
AjuI GAANNNNNNNTTGG 2 cut(s) 1878, 1910
AluBI AGCT 7 cut(s) 325, 352, 800, 912, 1028, 1302, 1837
AluI AGCT 7 cut(s) 325, 352, 800, 912, 1028, 1302, 1837
Alw21I GWGCWC 5 cut(s) 189, 802, 1080, 1144, 1553
Alw26I GTCTC 3 cut(s) 681, 1287, 1875
Alw44I GTGCAC 1 cut(s) 1549
AlwI GGATC 1 cut(s) 1835
Aor13HI TCCGGA 1 cut(s) 1454
AoxI GGCC 3 cut(s) 486, 594, 1636
ApaLI GTGCAC 1 cut(s) 1549
ApeKI GCWGC 5 cut(s) 38, 352, 475, 478, 1250
ApoI RAATTY 3 cut(s) 1180, 1532, 1602
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
AseI ATTAAT 1 cut(s) 1200
Asp700I GAANNNNTTC 1 cut(s) 1998
AspLEI GCGC 2 cut(s) 55, 780
AspS9I GGNCC 3 cut(s) 454, 620, 1305
AsuC2I CCSGG 1 cut(s) 1167
AsuHPI GGTGA 4 cut(s) 118, 575, 1492, 1631
AsuII TTCGAA 1 cut(s) 1536
AvaII GGWCC 3 cut(s) 454, 620, 1305
BaeGI GKGCMC 2 cut(s) 231, 1553
BanI GGYRCC 1 cut(s) 228
BanII GRGCYC 1 cut(s) 802
BarI GAAGNNNNNNTAC 2 cut(s) 663, 695
BbrPI CACGTG 1 cut(s) 2012
BbsI GAAGAC 1 cut(s) 1105
Bbv12I GWGCWC 5 cut(s) 189, 802, 1080, 1144, 1553
BbvI GCAGC 5 cut(s) 50, 339, 462, 465, 1262
BccI CCATC 3 cut(s) 488, 554, 602
BceAI ACGGC 1 cut(s) 161
BcgI CGANNNNNNTGC 4 cut(s) 1941, 1951, 1975, 1985
BciT130I CCWGG 1 cut(s) 1429
BciVI GTATCC 1 cut(s) 279
BcnI CCSGG 1 cut(s) 1167
BcoDI GTCTC 3 cut(s) 681, 1287, 1875
BcuI ACTAGT 1 cut(s) 997
BfaI CTAG 6 cut(s) 11, 356, 998, 1221, 1290, 1733
BfmI CTRYAG 1 cut(s) 305
BfrI CTTAAG 2 cut(s) 347, 805
BfuAI ACCTGC 2 cut(s) 404, 1779
BfuI GTATCC 1 cut(s) 279
BisI GCNGC 6 cut(s) 39, 353, 476, 479, 486, 1251
BlsI GCNGC 6 cut(s) 40, 354, 477, 480, 487, 1252
Bme1390I CCNGG 2 cut(s) 1167, 1429
Bme18I GGWCC 3 cut(s) 454, 620, 1305
BmgT120I GGNCC 3 cut(s) 454, 620, 1305
BmiI GGNNCC 3 cut(s) 230, 621, 744
BmrFI CCNGG 2 cut(s) 1167, 1429
BmrI ACTGGG 1 cut(s) 1081
BmsI GCATC 3 cut(s) 10, 102, 1971
BmuI ACTGGG 1 cut(s) 1081
BpiI GAAGAC 1 cut(s) 1105
BplI GAGNNNNNCTC 2 cut(s) 413, 445
BpmI CTGGAG 1 cut(s) 1143
Bpu14I TTCGAA 1 cut(s) 1536
BpuEI CTTGAG 1 cut(s) 907
BpuMI CCSGG 1 cut(s) 1167
BsaAI YACGTR 1 cut(s) 2012
BsaI GGTCTC 1 cut(s) 1875
BsaJI CCNNGG 2 cut(s) 875, 1392
BsaWI WCCGGW 2 cut(s) 212, 1454
Bsc4I CCNNNNNNNGG 6 cut(s) 934, 1053, 1136, 1166, 1374, 1398
Bse118I RCCGGY 2 cut(s) 488, 596
Bse1I ACTGG 5 cut(s) 1076, 1130, 1160, 1368, 2044
Bse3DI GCAATG 4 cut(s) 36, 447, 556, 1618
BseAI TCCGGA 1 cut(s) 1454
BseBI CCWGG 1 cut(s) 1429
BseDI CCNNGG 2 cut(s) 875, 1392
BseGI GGATG 1 cut(s) 613
BseLI CCNNNNNNNGG 6 cut(s) 934, 1053, 1136, 1166, 1374, 1398
BseMI GCAATG 4 cut(s) 36, 447, 556, 1618
BseMII CTCAG 4 cut(s) 156, 444, 945, 1799
BseNI ACTGG 5 cut(s) 1076, 1130, 1160, 1368, 2044
BseRI GAGGAG 1 cut(s) 737
BseSI GKGCMC 2 cut(s) 231, 1553
BseX3I CGGCCG 1 cut(s) 486
BseXI GCAGC 5 cut(s) 50, 339, 462, 465, 1262
BsgI GTGCAG 1 cut(s) 1751
Bsh1285I CGRYCG 1 cut(s) 489
BshFI GGCC 3 cut(s) 488, 596, 1638
BshNI GGYRCC 1 cut(s) 228
BsiEI CGRYCG 1 cut(s) 489
BsiHKAI GWGCWC 5 cut(s) 189, 802, 1080, 1144, 1553
BsiSI CCGG 5 cut(s) 213, 489, 597, 1167, 1455
BslFI GGGAC 4 cut(s) 261, 633, 1390, 1906
BslI CCNNNNNNNGG 6 cut(s) 934, 1053, 1136, 1166, 1374, 1398
BsmAI GTCTC 3 cut(s) 681, 1287, 1875
BsmBI CGTCTC 1 cut(s) 681
BsmFI GGGAC 4 cut(s) 261, 633, 1390, 1906
BsnI GGCC 3 cut(s) 488, 596, 1638
Bso31I GGTCTC 1 cut(s) 1875
Bsp119I TTCGAA 1 cut(s) 1536
Bsp1286I GDGCHC 6 cut(s) 189, 231, 802, 1080, 1144, 1553
Bsp13I TCCGGA 1 cut(s) 1454
Bsp143I GATC 4 cut(s) 282, 1022, 1840, 2016
Bsp19I CCATGG 1 cut(s) 875
BspACI CCGC 2 cut(s) 485, 657
BspANI GGCC 3 cut(s) 488, 596, 1638
BspCNI CTCAG 4 cut(s) 155, 443, 944, 1800
BspEI TCCGGA 1 cut(s) 1454
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 3 cut(s) 230, 621, 744
BspMAI CTGCAG 1 cut(s) 309
BspMI ACCTGC 2 cut(s) 404, 1779
BspPI GGATC 1 cut(s) 1835
BspT104I TTCGAA 1 cut(s) 1536
BspT107I GGYRCC 1 cut(s) 228
BspTI CTTAAG 2 cut(s) 347, 805
BspTNI GGTCTC 1 cut(s) 1875
BsrDI GCAATG 4 cut(s) 36, 447, 556, 1618
BsrFI RCCGGY 2 cut(s) 488, 596
BsrI ACTGG 5 cut(s) 1076, 1130, 1160, 1368, 2044
BssAI RCCGGY 2 cut(s) 488, 596
BssECI CCNNGG 2 cut(s) 875, 1392
BssMI GATC 4 cut(s) 282, 1022, 1840, 2016
BssNAI GTATAC 2 cut(s) 466, 1831
BssT1I CCWWGG 2 cut(s) 875, 1392
Bst1107I GTATAC 2 cut(s) 466, 1831
Bst2UI CCWGG 1 cut(s) 1429
Bst4CI ACNGT 2 cut(s) 1309, 1337
Bst6I CTCTTC 1 cut(s) 841
BstAFI CTTAAG 2 cut(s) 347, 805
BstAPI GCANNNNNTGC 2 cut(s) 20, 35
BstBAI YACGTR 1 cut(s) 2012
BstBI TTCGAA 1 cut(s) 1536
BstDEI CTNAG 7 cut(s) 142, 148, 251, 430, 931, 1808, 2032
BstDSI CCRYGG 1 cut(s) 875
BstENI CCTNNNNNAGG 2 cut(s) 932, 1396
BstF5I GGATG 1 cut(s) 613
BstHHI GCGC 2 cut(s) 55, 780
BstKTI GATC 4 cut(s) 285, 1025, 1843, 2019
BstMAI GTCTC 3 cut(s) 681, 1287, 1875
BstMBI GATC 4 cut(s) 282, 1022, 1840, 2016
BstMCI CGRYCG 1 cut(s) 489
BstMWI GCNNNNNNNGC 4 cut(s) 11, 20, 35, 1979
BstNI CCWGG 1 cut(s) 1429
BstNSI RCATGY 4 cut(s) 71, 97, 517, 1242
BstSCI CCNGG 2 cut(s) 1165, 1427
BstSFI CTRYAG 1 cut(s) 305
BstSLI GKGCMC 2 cut(s) 231, 1553
BstV1I GCAGC 5 cut(s) 50, 339, 462, 465, 1262
BstV2I GAAGAC 1 cut(s) 1105
BstXI CCANNNNNNTGG 1 cut(s) 63
BstZ17I GTATAC 2 cut(s) 466, 1831
BstZI CGGCCG 1 cut(s) 486
BsuI GTATCC 1 cut(s) 279
BsuRI GGCC 3 cut(s) 488, 596, 1638
BtgI CCRYGG 1 cut(s) 875
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 1 cut(s) 613
BtsI GCAGTG 3 cut(s) 289, 1414, 1763
BtsIMutI CAGTG 3 cut(s) 289, 1414, 1763
BveI ACCTGC 2 cut(s) 404, 1779
CciI TCATGA 1 cut(s) 418
CfoI GCGC 2 cut(s) 55, 780
Cfr10I RCCGGY 2 cut(s) 488, 596
Cfr13I GGNCC 3 cut(s) 454, 620, 1305
Csp6I GTAC 7 cut(s) 534, 637, 680, 884, 1263, 1371, 1868
CspCI CAANNNNNGTGG 4 cut(s) 308, 343, 429, 464
CviQI GTAC 7 cut(s) 534, 637, 680, 884, 1263, 1371, 1868
DdeI CTNAG 7 cut(s) 142, 148, 251, 430, 931, 1808, 2032
DpnI GATC 4 cut(s) 284, 1024, 1842, 2018
DpnII GATC 4 cut(s) 282, 1022, 1840, 2016
DraI TTTAAA 1 cut(s) 1449
DrdI GACNNNNNNGTC 1 cut(s) 1561
DseDI GACNNNNNNGTC 1 cut(s) 1561
EaeI YGGCCR 1 cut(s) 486
EagI CGGCCG 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 841
EarI CTCTTC 1 cut(s) 841
Ecl136II GAGCTC 1 cut(s) 800
EclXI CGGCCG 1 cut(s) 486
Eco130I CCWWGG 2 cut(s) 875, 1392
Eco24I GRGCYC 1 cut(s) 802
Eco31I GGTCTC 1 cut(s) 1875
Eco47I GGWCC 3 cut(s) 454, 620, 1305
Eco52I CGGCCG 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 800
Eco57I CTGAAG 1 cut(s) 771
Eco72I CACGTG 1 cut(s) 2012
EcoICRI GAGCTC 1 cut(s) 800
EcoNI CCTNNNNNAGG 2 cut(s) 932, 1396
EcoRI GAATTC 1 cut(s) 1532
EcoRII CCWGG 1 cut(s) 1427
EcoT14I CCWWGG 2 cut(s) 875, 1392
EcoT22I ATGCAT 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 802
ErhI CCWWGG 2 cut(s) 875, 1392
Esp3I CGTCTC 1 cut(s) 681
FaqI GGGAC 4 cut(s) 261, 633, 1390, 1906
FblI GTMKAC 2 cut(s) 465, 1830
Fnu4HI GCNGC 6 cut(s) 39, 353, 476, 479, 486, 1251
FokI GGATG 1 cut(s) 620
FriOI GRGCYC 1 cut(s) 802
Fsp4HI GCNGC 6 cut(s) 39, 353, 476, 479, 486, 1251
FspBI CTAG 6 cut(s) 11, 356, 998, 1221, 1290, 1733
GlaI GCGC 2 cut(s) 54, 779
GluI GCNGC 6 cut(s) 39, 353, 476, 479, 486, 1251
GsuI CTGGAG 1 cut(s) 1143
HaeIII GGCC 3 cut(s) 488, 596, 1638
HapII CCGG 5 cut(s) 213, 489, 597, 1167, 1455
HhaI GCGC 2 cut(s) 55, 780
Hin6I GCGC 2 cut(s) 53, 778
HinP1I GCGC 2 cut(s) 53, 778
HindIII AAGCTT 1 cut(s) 1835
HinfI GANTC 3 cut(s) 157, 1040, 1381
HpaII CCGG 5 cut(s) 213, 489, 597, 1167, 1455
HphI GGTGA 4 cut(s) 118, 575, 1492, 1631
Hpy166II GTNNAC 8 cut(s) 454, 466, 534, 866, 886, 1351, 1551, 1831
Hpy188I TCNGA 8 cut(s) 145, 156, 433, 934, 1020, 1567, 1682, 1809
Hpy188III TCNNGA 5 cut(s) 419, 856, 1047, 1455, 1844
Hpy8I GTNNAC 8 cut(s) 454, 466, 534, 866, 886, 1351, 1551, 1831
HpyCH4III ACNGT 2 cut(s) 1309, 1337
HpyCH4IV ACGT 2 cut(s) 1728, 2011
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 20, 35, 1979
HpyF3I CTNAG 7 cut(s) 142, 148, 251, 430, 931, 1808, 2032
HpySE526I ACGT 2 cut(s) 1728, 2011
HspAI GCGC 2 cut(s) 53, 778
Kpn2I TCCGGA 1 cut(s) 1454
KroI GCCGGC 2 cut(s) 488, 596
KroNI GCCGGC 2 cut(s) 490, 598
Kzo9I GATC 4 cut(s) 282, 1022, 1840, 2016
LmnI GCTCC 4 cut(s) 80, 184, 1075, 1139
Lsp1109I GCAGC 5 cut(s) 50, 339, 462, 465, 1262
LweI GCATC 3 cut(s) 10, 102, 1971
MaeI CTAG 6 cut(s) 11, 356, 998, 1221, 1290, 1733
MaeII ACGT 2 cut(s) 1728, 2011
MaeIII GTNAC 4 cut(s) 73, 124, 659, 1518
MalI GATC 4 cut(s) 284, 1024, 1842, 2018
MboI GATC 4 cut(s) 282, 1022, 1840, 2016
MfeI CAATTG 1 cut(s) 1583
MhlI GDGCHC 6 cut(s) 189, 231, 802, 1080, 1144, 1553
MlyI GAGTC 1 cut(s) 1375
MmeI TCCRAC 2 cut(s) 345, 1723
Mph1103I ATGCAT 1 cut(s) 519
MroI TCCGGA 1 cut(s) 1454
MroNI GCCGGC 2 cut(s) 488, 596
MroXI GAANNNNTTC 1 cut(s) 1998
MseI TTAA 5 cut(s) 348, 774, 806, 1200, 1448
MspA1I CMGCKG 1 cut(s) 325
MspCI CTTAAG 2 cut(s) 347, 805
MspI CCGG 5 cut(s) 213, 489, 597, 1167, 1455
MspR9I CCNGG 2 cut(s) 1167, 1429
MunI CAATTG 1 cut(s) 1583
MvaI CCWGG 1 cut(s) 1429
MwoI GCNNNNNNNGC 4 cut(s) 11, 20, 35, 1979
NaeI GCCGGC 2 cut(s) 490, 598
NciI CCSGG 1 cut(s) 1167
NcoI CCATGG 1 cut(s) 875
NdeII GATC 4 cut(s) 282, 1022, 1840, 2016
NgoMIV GCCGGC 2 cut(s) 488, 596
NlaIV GGNNCC 3 cut(s) 230, 621, 744
NmuCI GTSAC 1 cut(s) 124
NsiI ATGCAT 1 cut(s) 519
NspI RCATGY 4 cut(s) 71, 97, 517, 1242
NspV TTCGAA 1 cut(s) 1536
PaeI GCATGC 2 cut(s) 71, 517
PagI TCATGA 1 cut(s) 418
PaqCI CACCTGC 1 cut(s) 1779
PciI ACATGT 2 cut(s) 93, 1238
PdiI GCCGGC 2 cut(s) 490, 598
PdmI GAANNNNTTC 1 cut(s) 1998
PfeI GAWTC 2 cut(s) 157, 1040
PflMI CCANNNNNTGG 1 cut(s) 1053
PkrI GCNGC 6 cut(s) 40, 354, 477, 480, 487, 1252
PleI GAGTC 1 cut(s) 1375
PmaCI CACGTG 1 cut(s) 2012
PmlI CACGTG 1 cut(s) 2012
PpsI GAGTC 1 cut(s) 1375
Ppu21I YACGTR 1 cut(s) 2012
PscI ACATGT 2 cut(s) 93, 1238
PshBI ATTAAT 1 cut(s) 1200
Psp124BI GAGCTC 1 cut(s) 802
Psp1406I AACGTT 1 cut(s) 1728
Psp6I CCWGG 1 cut(s) 1427
PspCI CACGTG 1 cut(s) 2012
PspGI CCWGG 1 cut(s) 1427
PspN4I GGNNCC 3 cut(s) 230, 621, 744
PspPI GGNCC 3 cut(s) 454, 620, 1305
PstI CTGCAG 1 cut(s) 309
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 7 cut(s) 535, 638, 681, 885, 1264, 1372, 1869
RsaNI GTAC 7 cut(s) 534, 637, 680, 884, 1263, 1371, 1868
SacI GAGCTC 1 cut(s) 802
SaqAI TTAA 5 cut(s) 348, 774, 806, 1200, 1448
SatI GCNGC 6 cut(s) 39, 353, 476, 479, 486, 1251
Sau3AI GATC 4 cut(s) 282, 1022, 1840, 2016
Sau96I GGNCC 3 cut(s) 454, 620, 1305
SchI GAGTC 1 cut(s) 1375
ScrFI CCNGG 2 cut(s) 1167, 1429
SduI GDGCHC 6 cut(s) 189, 231, 802, 1080, 1144, 1553
SfaNI GCATC 3 cut(s) 10, 102, 1971
SfcI CTRYAG 1 cut(s) 305
SfuI TTCGAA 1 cut(s) 1536
SinI GGWCC 3 cut(s) 454, 620, 1305
SmlI CTYRAG 3 cut(s) 347, 805, 922
SmoI CTYRAG 3 cut(s) 347, 805, 922
SpeI ACTAGT 1 cut(s) 997
SphI GCATGC 2 cut(s) 71, 517
SsiI CCGC 2 cut(s) 485, 657
SspI AATATT 1 cut(s) 1890
SspMI CTAG 6 cut(s) 11, 356, 998, 1221, 1290, 1733
SstI GAGCTC 1 cut(s) 802
StyD4I CCNGG 2 cut(s) 1165, 1427
StyI CCWWGG 2 cut(s) 875, 1392
TaaI ACNGT 2 cut(s) 1309, 1337
TaiI ACGT 2 cut(s) 1731, 2014
TaqI TCGA 5 cut(s) 796, 953, 983, 1038, 1536
TatI WGTACW 2 cut(s) 883, 1370
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 2 cut(s) 157, 1040
Tru1I TTAA 5 cut(s) 348, 774, 806, 1200, 1448
Tru9I TTAA 5 cut(s) 348, 774, 806, 1200, 1448
TscAI CASTG 3 cut(s) 296, 1414, 1770
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 5 cut(s) 38, 352, 475, 478, 1250
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 6 cut(s) 152, 1341, 1476, 1527, 1905, 2007
TspGWI ACGGA 2 cut(s) 602, 697
TspRI CASTG 3 cut(s) 296, 1414, 1770
Van91I CCANNNNNTGG 1 cut(s) 1053
Vha464I CTTAAG 2 cut(s) 347, 805
VneI GTGCAC 1 cut(s) 1549
VpaK11BI GGWCC 3 cut(s) 454, 620, 1305
VspI ATTAAT 1 cut(s) 1200
XagI CCTNNNNNAGG 2 cut(s) 932, 1396
XapI RAATTY 3 cut(s) 1180, 1532, 1602
XceI RCATGY 4 cut(s) 71, 97, 517, 1242
XcmI CCANNNNNNNNNTGG 2 cut(s) 873, 1054
XmiI GTMKAC 2 cut(s) 465, 1830
XmnI GAANNNNTTC 1 cut(s) 1998
XspI CTAG 6 cut(s) 11, 356, 998, 1221, 1290, 1733
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.