FvH4_2g04271

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000144
Physical Location & Seq
Reverse (-)
3341763 .. 3343759
1997 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04271.t1

Sequence Viewer

Length: 1584 bp
ATGCATGATGCATGCTTGGTTGTCAGTTTCACATCAAGTCTATGGTCTATTTTGCTTTGTTTTCGCGGAGAAAGGAAAATCAATATGTTGCGGCAAAGGAAGAATATCCTGCAGAAAAGGAAGGATATGGTGCTGATTGAGAGAAAGGGAAAGATGACTGATAGAAAGAAAAAGGAAGAGGAAAAGGAAAAAGAAAGGCAAAATATAAAATTGGGAAATGGTTGTAGCTCTTGGCAGACACCACCGGAAGATGTCATGGAGAAGATTATACGGTTCGTCGAGTTTGCTGACCAGAGAAGGTTAGGTCAGTTCAGCAAGTCTTGGAGGTCAGTTGTTTATCAAACATTCATGCGTAGAGCTCGTCATCAACTCCCATGGTTAATACTATCTGAATCGCCGAATTGCCGGGTCAACAAGTATGTGAGCTTTCTAACCTTCCCTGAGAACAAGCTCTGTAAGGGCAAAGTTCTCAAATTAAAACTGCCTAAACAACTTCAAAGAGGGTGGATATACGGGTCGTCTAAAGGTTGGTTGATCATTGTCAAAGAAAAGGGACTAGACTCTGAGATGTGTGTACTGAACCCAATTTCAGGAATCCTACATAAACTTCCGCCCTTGAGAACAGTTCCGTCTTTCAGAAAGTTTATCAAAACCAGAAGATGGGAACTCTTAGGTGCCGAAGGATTTTGTCTATGCATTAAAATGTCTTCTACCTCGAATGATTTTAATTCTTCAAGCGATTTTATTGTAGCTGCAGTTTTGGATGATAGGAAGATTTTAGGTTTGTGCAGACCAGGAGACAAAAAATGGAGTGTCTTCCTGATATCAAACTTCATCATTGATATATTGTTTTCTTCTGGCATGTTGTATGCTTTGGTTCGAAGTAAACAAAATGACGAAGGTGTCGCACCCACTCGCACCTTAAACATTCCCCTTTATGGTGCTGAACACTTAAAGTTGAAAGTGGTCTTTGACAAGGTCTTTGACAAGGTTGAATTACCCACTCGCACCTTAAACTTTTCCAAGGCAACATGTTGGTCGATGTTGTTAGAATCAACCAGCAAGGAAGTTATGTTAATCCATCAAATAACTGGTTATGCCATGAAGAGAATAAATGATGGTGACAAACAAATCAATGAGAATGGTGACAATCATGACCTAAATGTTGAGGGAGACATTGATGGTGTTGAAGGCAATACTGACGATGAAGGTGGTGGTGGTGACGGTGATAATGAAGTAGTCCATGAGAATACTAGAGATGTACGTTATTGCACGAGGAGTTTTAGAACATACAACATAGATGCAGAAAACAACAATTTTCATATGATGCACTTCTTGGGCAACCAAATTATATTTTGTGGAAACGATGGTGCTTTCTCCCTCCCTGCTAGTAACATCAAAGAACTAGGAAATAATTGTGTCTACTATGCAATGAATTTGGGGTGGGAGGTGGACTTACCAAAGACATATATATCTGATGAGTTTGGCATATTCTACTTGGATGGTCAGAATACTGAGAGACCATTTGAATGTATGAAAATTTCAATCAAGTGTCTAGGGAGTTGGTTCACTCCAACGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

528

Amino Acids

60.56

Weight (kDa)

8.91

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 153 - 485 8.9e-27 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 902
AccB1I GGYRCC 1 cut(s) 674
AccB7I CCANNNNNTGG 1 cut(s) 660
AccI GTMKAC 1 cut(s) 1422
AccII CGCG 1 cut(s) 66
AciI CCGC 3 cut(s) 66, 91, 611
AclI AACGTT 1 cut(s) 1577
AcsI RAATTY 2 cut(s) 1435, 1539
AfaI GTAC 2 cut(s) 576, 1263
AfiI CCNNNNNNNGG 3 cut(s) 590, 660, 938
AflIII ACRYGT 1 cut(s) 1031
AgsI TTSAA 7 cut(s) 497, 735, 961, 995, 1190, 1529, 1545
AjnI CCWGG 1 cut(s) 793
AluBI AGCT 5 cut(s) 228, 359, 426, 451, 752
AluI AGCT 5 cut(s) 228, 359, 426, 451, 752
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 3 cut(s) 792, 1167, 1513
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 2 cut(s) 1435, 1539
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 4 cut(s) 1133, 1157, 1232, 1238
AsuII TTCGAA 1 cut(s) 880
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 1 cut(s) 361
BauI CACGAG 1 cut(s) 1273
BbsI GAAGAC 2 cut(s) 699, 808
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 1 cut(s) 739
BccI CCATC 6 cut(s) 654, 1089, 1112, 1175, 1361, 1496
BciT130I CCWGG 1 cut(s) 795
BclI TGATCA 1 cut(s) 534
BcnI CCSGG 1 cut(s) 407
BcoDI GTCTC 3 cut(s) 792, 1167, 1513
BfaI CTAG 5 cut(s) 557, 1254, 1389, 1406, 1556
BfmI CTRYAG 2 cut(s) 110, 753
BisI GCNGC 2 cut(s) 92, 753
BlsI GCNGC 2 cut(s) 93, 754
Bme1390I CCNGG 2 cut(s) 407, 795
BmiI GGNNCC 1 cut(s) 676
BmrFI CCNGG 2 cut(s) 407, 795
BmsI GCATC 2 cut(s) 1291, 1317
BpiI GAAGAC 2 cut(s) 699, 808
Bpu14I TTCGAA 1 cut(s) 880
BpuEI CTTGAG 1 cut(s) 637
BpuMI CCSGG 1 cut(s) 407
BsaI GGTCTC 1 cut(s) 1513
BsaJI CCNNGG 2 cut(s) 374, 1023
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 3 cut(s) 590, 660, 938
Bse1I ACTGG 1 cut(s) 1096
Bse3DI GCAATG 1 cut(s) 1437
BseBI CCWGG 1 cut(s) 795
BseDI CCNNGG 2 cut(s) 374, 1023
BseGI GGATG 2 cut(s) 769, 1507
BseLI CCNNNNNNNGG 3 cut(s) 590, 660, 938
BseMI GCAATG 1 cut(s) 1437
BseMII CTCAG 3 cut(s) 432, 555, 1506
BseNI ACTGG 1 cut(s) 1096
BseRI GAGGAG 1 cut(s) 1291
BseXI GCAGC 1 cut(s) 739
BsgI GTGCAG 1 cut(s) 808
Bsh1236I CGCG 1 cut(s) 66
BshNI GGYRCC 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 361
BsiSI CCGG 2 cut(s) 245, 406
BslFI GGGAC 1 cut(s) 567
BslI CCNNNNNNNGG 3 cut(s) 590, 660, 938
BsmAI GTCTC 3 cut(s) 792, 1167, 1513
BsmFI GGGAC 1 cut(s) 567
Bso31I GGTCTC 1 cut(s) 1513
Bsp119I TTCGAA 1 cut(s) 880
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 1 cut(s) 534
Bsp19I CCATGG 1 cut(s) 374
BspACI CCGC 3 cut(s) 66, 91, 611
BspCNI CTCAG 3 cut(s) 433, 556, 1507
BspFNI CGCG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 1153
BspLI GGNNCC 1 cut(s) 676
BspMAI CTGCAG 2 cut(s) 114, 757
BspT104I TTCGAA 1 cut(s) 880
BspT107I GGYRCC 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 1513
BsrDI GCAATG 1 cut(s) 1437
BsrI ACTGG 1 cut(s) 1096
BssECI CCNNGG 2 cut(s) 374, 1023
BssMI GATC 1 cut(s) 534
BssSI CACGAG 1 cut(s) 1273
BssT1I CCWWGG 2 cut(s) 374, 1023
Bst2BI CACGAG 1 cut(s) 1273
Bst2UI CCWGG 1 cut(s) 795
Bst4CI ACNGT 3 cut(s) 273, 625, 1226
Bst6I CTCTTC 2 cut(s) 171, 1100
BstBI TTCGAA 1 cut(s) 880
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 4 cut(s) 441, 564, 670, 1515
BstDSI CCRYGG 1 cut(s) 374
BstF5I GGATG 2 cut(s) 769, 1507
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 1 cut(s) 537
BstMAI GTCTC 3 cut(s) 792, 1167, 1513
BstMBI GATC 1 cut(s) 534
BstNI CCWGG 1 cut(s) 795
BstNSI RCATGY 3 cut(s) 15, 865, 1035
BstSCI CCNGG 2 cut(s) 405, 793
BstSFI CTRYAG 2 cut(s) 110, 753
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 739
BstV2I GAAGAC 2 cut(s) 699, 808
BtgI CCRYGG 1 cut(s) 374
BtsCI GGATG 2 cut(s) 769, 1507
Cac8I GCNNGC 1 cut(s) 13
CciI TCATGA 1 cut(s) 1153
Csp6I GTAC 2 cut(s) 575, 1262
CspCI CAANNNNNGTGG 2 cut(s) 485, 520
CviJI RGCY 5 cut(s) 228, 359, 426, 451, 752
CviKI_1 RGCY 5 cut(s) 228, 359, 426, 451, 752
CviQI GTAC 2 cut(s) 575, 1262
DdeI CTNAG 4 cut(s) 441, 564, 670, 1515
DpnI GATC 1 cut(s) 536
DpnII GATC 1 cut(s) 534
DrdI GACNNNNNNGTC 1 cut(s) 902
DseDI GACNNNNNNGTC 1 cut(s) 902
Eam1104I CTCTTC 2 cut(s) 171, 1100
EarI CTCTTC 2 cut(s) 171, 1100
EciI GGCGGA 1 cut(s) 600
Ecl136II GAGCTC 1 cut(s) 359
Eco130I CCWWGG 2 cut(s) 374, 1023
Eco24I GRGCYC 1 cut(s) 361
Eco31I GGTCTC 1 cut(s) 1513
Eco32I GATATC 1 cut(s) 825
Eco53kI GAGCTC 1 cut(s) 359
EcoICRI GAGCTC 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 793
EcoRV GATATC 1 cut(s) 825
EcoT14I CCWWGG 2 cut(s) 374, 1023
EcoT22I ATGCAT 3 cut(s) 6, 13, 698
EcoT38I GRGCYC 1 cut(s) 361
ErhI CCWWGG 2 cut(s) 374, 1023
FaqI GGGAC 1 cut(s) 567
FauNDI CATATG 1 cut(s) 1323
FbaI TGATCA 1 cut(s) 534
FblI GTMKAC 1 cut(s) 1422
Fnu4HI GCNGC 2 cut(s) 92, 753
FokI GGATG 2 cut(s) 776, 1514
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 2 cut(s) 92, 753
FspBI CTAG 5 cut(s) 557, 1254, 1389, 1406, 1556
GluI GCNGC 2 cut(s) 92, 753
HapII CCGG 2 cut(s) 245, 406
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 4 cut(s) 392, 560, 594, 1052
HpaII CCGG 2 cut(s) 245, 406
HphI GGTGA 4 cut(s) 1133, 1157, 1232, 1238
Hpy166II GTNNAC 6 cut(s) 412, 575, 887, 1423, 1453, 1569
Hpy188I TCNGA 5 cut(s) 391, 565, 638, 1477, 1509
Hpy188III TCNNGA 3 cut(s) 591, 820, 1154
Hpy8I GTNNAC 6 cut(s) 412, 575, 887, 1423, 1453, 1569
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 7 cut(s) 115, 291, 445, 674, 893, 1184, 1202
HpyCH4III ACNGT 3 cut(s) 273, 625, 1226
HpyCH4IV ACGT 2 cut(s) 1264, 1577
HpyF3I CTNAG 4 cut(s) 441, 564, 670, 1515
HpySE526I ACGT 2 cut(s) 1264, 1577
Ksp22I TGATCA 1 cut(s) 534
Kzo9I GATC 1 cut(s) 534
Lsp1109I GCAGC 1 cut(s) 739
LweI GCATC 2 cut(s) 1291, 1317
MaeI CTAG 5 cut(s) 557, 1254, 1389, 1406, 1556
MaeII ACGT 2 cut(s) 1264, 1577
MaeIII GTNAC 4 cut(s) 1121, 1145, 1220, 1391
MalI GATC 1 cut(s) 536
MboI GATC 1 cut(s) 534
MhlI GDGCHC 1 cut(s) 361
MlyI GAGTC 1 cut(s) 554
MnlI CCTC 8 cut(s) 172, 318, 494, 724, 1162, 1269, 1391, 1441
Mph1103I ATGCAT 3 cut(s) 6, 13, 698
MseI TTAA 8 cut(s) 380, 476, 699, 726, 923, 953, 1013, 1076
MslI CAYNNNNRTG 2 cut(s) 701, 1528
MspI CCGG 2 cut(s) 245, 406
MspR9I CCNGG 2 cut(s) 407, 795
MvaI CCWGG 1 cut(s) 795
MvnI CGCG 1 cut(s) 66
NciI CCSGG 1 cut(s) 407
NcoI CCATGG 1 cut(s) 374
NdeI CATATG 1 cut(s) 1323
NdeII GATC 1 cut(s) 534
NlaIV GGNNCC 1 cut(s) 676
NmuCI GTSAC 3 cut(s) 1121, 1145, 1220
NsiI ATGCAT 3 cut(s) 6, 13, 698
NspI RCATGY 3 cut(s) 15, 865, 1035
NspV TTCGAA 1 cut(s) 880
PaeI GCATGC 1 cut(s) 15
PagI TCATGA 1 cut(s) 1153
PciI ACATGT 1 cut(s) 1031
PfeI GAWTC 3 cut(s) 392, 594, 1052
PflFI GACNNNGTC 1 cut(s) 977
PflMI CCANNNNNTGG 1 cut(s) 660
PkrI GCNGC 2 cut(s) 93, 754
PleI GAGTC 1 cut(s) 554
PpsI GAGTC 1 cut(s) 554
PscI ACATGT 1 cut(s) 1031
Psp124BI GAGCTC 1 cut(s) 361
Psp1406I AACGTT 1 cut(s) 1577
Psp6I CCWGG 1 cut(s) 793
PspGI CCWGG 1 cut(s) 793
PspN4I GGNNCC 1 cut(s) 676
PstI CTGCAG 2 cut(s) 114, 757
PsyI GACNNNGTC 1 cut(s) 977
RsaI GTAC 2 cut(s) 576, 1263
RsaNI GTAC 2 cut(s) 575, 1262
RseI CAYNNNNRTG 2 cut(s) 701, 1528
SacI GAGCTC 1 cut(s) 361
SaqAI TTAA 8 cut(s) 380, 476, 699, 726, 923, 953, 1013, 1076
SatI GCNGC 2 cut(s) 92, 753
Sau3AI GATC 1 cut(s) 534
SchI GAGTC 1 cut(s) 554
ScrFI CCNGG 2 cut(s) 407, 795
SduI GDGCHC 1 cut(s) 361
SfaNI GCATC 2 cut(s) 1291, 1317
SfcI CTRYAG 2 cut(s) 110, 753
SfuI TTCGAA 1 cut(s) 880
SmiMI CAYNNNNRTG 2 cut(s) 701, 1528
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
SphI GCATGC 1 cut(s) 15
SsiI CCGC 3 cut(s) 66, 91, 611
SspMI CTAG 5 cut(s) 557, 1254, 1389, 1406, 1556
SstI GAGCTC 1 cut(s) 361
StyD4I CCNGG 2 cut(s) 405, 793
StyI CCWWGG 2 cut(s) 374, 1023
TaaI ACNGT 3 cut(s) 273, 625, 1226
TaiI ACGT 2 cut(s) 1267, 1580
TaqI TCGA 4 cut(s) 279, 716, 880, 1040
TatI WGTACW 1 cut(s) 574
TauI GCSGC 1 cut(s) 94
TfiI GAWTC 3 cut(s) 392, 594, 1052
Tru1I TTAA 8 cut(s) 380, 476, 699, 726, 923, 953, 1013, 1076
Tru9I TTAA 8 cut(s) 380, 476, 699, 726, 923, 953, 1013, 1076
TseFI GTSAC 3 cut(s) 1121, 1145, 1220
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 3 cut(s) 1121, 1145, 1220
TspDTI ATGAA 8 cut(s) 337, 823, 1118, 1221, 1248, 1310, 1448, 1550
TspGWI ACGGA 1 cut(s) 618
Tth111I GACNNNGTC 1 cut(s) 977
Van91I CCANNNNNTGG 1 cut(s) 660
XapI RAATTY 2 cut(s) 1435, 1539
XceI RCATGY 3 cut(s) 15, 865, 1035
XcmI CCANNNNNNNNNTGG 1 cut(s) 1088
XmiI GTMKAC 1 cut(s) 1422
XspI CTAG 5 cut(s) 557, 1254, 1389, 1406, 1556
Zsp2I ATGCAT 3 cut(s) 6, 13, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.