Rh6BG062800

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000144
Physical Location & Seq
Forward (+)
9850013 .. 9851498
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG062800.1

Sequence Viewer

Length: 1443 bp
ATGGTTACTAATGAGTATAGTTGTTGGCAGACACCACCTGTAGAGATCATGGAGAACATTCTACAACGTGTCAATTTAGGAGATCGAATACGGTTAAGAAACTCTTGCAAGTATTGGAAGTCAATTGTTATGCGAAGAGACATCCCCGGGGCTCCGCATGAACTCCCATGGTTACTGCTGCCTCAAGCCCCCAAATGCAGCAACAAGTGCTTGAGCTTTGCCAGCCTCACTGAGAGGAAAGAAGTCAAGTTAAAGCTTCCTAAGGAAGTTCGAGGAAGCTGGATTTATGGGTCTTCTAAAGGTTGGTTGATCATGGTCAAGGAAAAAGGATTACAGTCTACGATGTGTCTACTTAACCCAATTTCAGGAGCCCTACACAAACTTCCGCCGTTGAGGACAATTCCATCTTTCAAAAAGATTGTAAAAACGAGGCAATGGAAACGTTTTGGTGCCAATATTTTCCTCTTGTATGTTGCATTACTTACCCCTCATGATCTTAATTCTTCAGGCCATTTTACGGTAGCCATAATCTTTGAGGATTTCAAGGGCCAGAAGACATTGGGTTTATGCAAACTTGAAGACCTAACATGGAGTCTCTTCCGGGTATTCGACAACAACGACCGCCTTGAGCTTGTGGGTATACTGTTTTCTCGAGGCATTCTGTATGCTTTAGTCAATAGTCAAAAGGATGGCTTGGTAGCAGCTACTCGTATTTTTAACTTTGCAGATCATGAAGTGAAGTTGAAATTGATTCACGACAAGCATGAACACAAGAATGTGAGAGTTGATGATTTTAATAGTGACTATGCAAGAGTTTCCAATGCAAACTATCGATCAATATTGCTAGAATCCACCAGCAATGAAGTCTTGGTAATCCATCAAATGGTAGATTATGTTTTGTGCAGAGAAGGTGCTGGTGATTTCAATGACATTGAAGAAGACGACGGCAATATTATTGTGGATAATCAGGAAGGCGTTGATCAAGATAATGATGGTGATGAAGGCAATCATGAGAATGATCATGAGGAAGGCAACTTGGAAGCTAATAATCAAGATGGAGGAGAAATTGATGATGTTGAATATGTTATTGATGAAGACATGCCCGGCTTTAATCCACATGTCACAACAAGGAGTTTTAGAGTATACAAGATTGATCCAAATAATAACAATTTGCTTCCAGTGCAGAACTTGGGGAACCAAGTGTTCTTTTTGGGAGATGGTGGTGGTTCTGTCTCTCTTCCGGCTGGTAATTTCCCAGAAGTAAAAAGGAATTGTATTTATTTTGCAACAAATTATGTATGGAGTATAGAATTGACTCCTAAAACATATAGGTCTCGTGAGATCGGCATATTCTACTTAGATGGTGAAAGAATTATACGGCCTTTTTCGAGTGTCAGAATATCATTGTTGTATCAAGCCACATGGTTCACTCCAAGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

480

Amino Acids

54.8

Weight (kDa)

5.8

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 13 - 46 6.5e-06 F-box domain
Beta-prop_KIB1-4 PF03478 73 - 451 5.1e-31 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 449
AccB7I CCANNNNNTGG 1 cut(s) 883
AccI GTMKAC 4 cut(s) 338, 349, 640, 1143
AciI CCGC 3 cut(s) 155, 386, 622
AclI AACGTT 1 cut(s) 442
AclWI GGATC 1 cut(s) 1148
AcuI CTGAAG 1 cut(s) 489
AfiI CCNNNNNNNGG 3 cut(s) 365, 517, 883
AflIII ACRYGT 2 cut(s) 67, 1117
AgsI TTSAA 7 cut(s) 412, 544, 578, 745, 925, 935, 1079
AluBI AGCT 6 cut(s) 216, 256, 279, 631, 704, 1043
AluI AGCT 6 cut(s) 216, 256, 279, 631, 704, 1043
Alw26I GTCTC 4 cut(s) 132, 599, 1237, 1338
AlwI GGATC 1 cut(s) 1148
Ama87I CYCGRG 2 cut(s) 146, 651
AoxI GGCC 3 cut(s) 508, 547, 1379
ApeKI GCWGC 3 cut(s) 178, 198, 701
AspS9I GGNCC 1 cut(s) 547
AsuC2I CCSGG 4 cut(s) 147, 148, 602, 1104
AsuHPI GGTGA 3 cut(s) 929, 1007, 1376
AvaI CYCGRG 2 cut(s) 146, 651
AxyI CCTNAGG 1 cut(s) 261
BanI GGYRCC 1 cut(s) 449
BanII GRGCYC 2 cut(s) 154, 373
BauI CACGAG 1 cut(s) 1335
BbsI GAAGAC 5 cut(s) 285, 560, 585, 945, 1101
BbvI GCAGC 3 cut(s) 165, 210, 713
BccI CCATC 7 cut(s) 412, 683, 885, 986, 1049, 1211, 1355
BceAI ACGGC 3 cut(s) 373, 961, 1394
BclI TGATCA 3 cut(s) 309, 979, 1018
BcnI CCSGG 4 cut(s) 147, 148, 602, 1104
BcoDI GTCTC 4 cut(s) 132, 599, 1237, 1338
BfaI CTAG 1 cut(s) 845
BfmI CTRYAG 1 cut(s) 39
BisI GCNGC 3 cut(s) 179, 199, 702
BlsI GCNGC 3 cut(s) 180, 200, 703
Bme1390I CCNGG 4 cut(s) 147, 148, 602, 1104
BmeT110I CYCGRG 2 cut(s) 146, 651
BmgT120I GGNCC 1 cut(s) 547
BmiI GGNNCC 4 cut(s) 153, 370, 451, 1196
BmrFI CCNGG 4 cut(s) 147, 148, 602, 1104
BpiI GAAGAC 5 cut(s) 285, 560, 585, 945, 1101
BpuEI CTTGAG 3 cut(s) 168, 232, 647
BpuMI CCSGG 4 cut(s) 147, 148, 602, 1104
Bsa29I ATCGAT 1 cut(s) 832
BsaI GGTCTC 1 cut(s) 1338
BsaJI CCNNGG 4 cut(s) 145, 146, 147, 167
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 517, 883
Bse1I ACTGG 1 cut(s) 1178
Bse21I CCTNAGG 1 cut(s) 261
Bse3DI GCAATG 2 cut(s) 440, 865
BseCI ATCGAT 1 cut(s) 832
BseDI CCNNGG 4 cut(s) 145, 146, 147, 167
BseGI GGATG 2 cut(s) 141, 694
BseLI CCNNNNNNNGG 3 cut(s) 365, 517, 883
BseMI GCAATG 2 cut(s) 440, 865
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 1178
BseRI GAGGAG 1 cut(s) 1074
BseXI GCAGC 3 cut(s) 165, 210, 713
BsgI GTGCAG 2 cut(s) 922, 1202
Bsh1285I CGRYCG 1 cut(s) 622
BshFI GGCC 3 cut(s) 510, 549, 1381
BshNI GGYRCC 1 cut(s) 449
BshVI ATCGAT 1 cut(s) 832
BsiEI CGRYCG 1 cut(s) 622
BsiHKCI CYCGRG 2 cut(s) 146, 651
BsiSI CCGG 4 cut(s) 147, 601, 1104, 1241
BslI CCNNNNNNNGG 3 cut(s) 365, 517, 883
BsmAI GTCTC 4 cut(s) 132, 599, 1237, 1338
BsmI GAATGC 1 cut(s) 657
BsnI GGCC 3 cut(s) 510, 549, 1381
Bso31I GGTCTC 1 cut(s) 1338
BsoBI CYCGRG 2 cut(s) 146, 651
Bsp1286I GDGCHC 2 cut(s) 154, 373
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 3 cut(s) 155, 386, 622
BspANI GGCC 3 cut(s) 510, 549, 1381
BspCNI CTCAG 1 cut(s) 223
BspDI ATCGAT 1 cut(s) 832
BspHI TCATGA 4 cut(s) 490, 730, 1009, 1021
BspLI GGNNCC 4 cut(s) 153, 370, 451, 1196
BspPI GGATC 1 cut(s) 1148
BspT107I GGYRCC 1 cut(s) 449
BspTNI GGTCTC 1 cut(s) 1338
BsrDI GCAATG 2 cut(s) 440, 865
BsrI ACTGG 1 cut(s) 1178
BssECI CCNNGG 4 cut(s) 145, 146, 147, 167
BssNAI GTATAC 2 cut(s) 641, 1144
BssSI CACGAG 1 cut(s) 1335
BssT1I CCWWGG 1 cut(s) 167
Bst1107I GTATAC 2 cut(s) 641, 1144
Bst2BI CACGAG 1 cut(s) 1335
Bst4CI ACNGT 4 cut(s) 93, 336, 520, 645
Bst6I CTCTTC 3 cut(s) 130, 602, 1242
BstAPI GCANNNNNTGC 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 3 cut(s) 231, 261, 1357
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 2 cut(s) 141, 694
BstMAI GTCTC 4 cut(s) 132, 599, 1237, 1338
BstMCI CGRYCG 1 cut(s) 622
BstMWI GCNNNNNNNGC 3 cut(s) 207, 222, 1180
BstNSI RCATGY 2 cut(s) 1102, 1121
BstSCI CCNGG 4 cut(s) 145, 146, 600, 1102
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 165, 210, 713
BstV2I GAAGAC 5 cut(s) 285, 560, 585, 945, 1101
BstZ17I GTATAC 2 cut(s) 641, 1144
Bsu15I ATCGAT 1 cut(s) 832
Bsu36I CCTNAGG 1 cut(s) 261
BsuRI GGCC 3 cut(s) 510, 549, 1381
BsuTUI ATCGAT 1 cut(s) 832
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 2 cut(s) 141, 694
BtsIMutI CAGTG 2 cut(s) 228, 1185
Cac8I GCNNGC 1 cut(s) 223
CciI TCATGA 4 cut(s) 490, 730, 1009, 1021
Cfr13I GGNCC 1 cut(s) 547
Cfr9I CCCGGG 1 cut(s) 146
ClaI ATCGAT 1 cut(s) 832
DdeI CTNAG 3 cut(s) 231, 261, 1357
Eam1104I CTCTTC 3 cut(s) 130, 602, 1242
EarI CTCTTC 3 cut(s) 130, 602, 1242
EciI GGCGGA 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 167
Eco24I GRGCYC 2 cut(s) 154, 373
Eco31I GGTCTC 1 cut(s) 1338
Eco57I CTGAAG 1 cut(s) 489
Eco81I CCTNAGG 1 cut(s) 261
Eco88I CYCGRG 2 cut(s) 146, 651
EcoT14I CCWWGG 1 cut(s) 167
EcoT38I GRGCYC 2 cut(s) 154, 373
ErhI CCWWGG 1 cut(s) 167
FalI AAGNNNNNCTT 4 cut(s) 88, 120, 677, 709
FbaI TGATCA 3 cut(s) 309, 979, 1018
FblI GTMKAC 4 cut(s) 338, 349, 640, 1143
Fnu4HI GCNGC 3 cut(s) 179, 199, 702
FokI GGATG 2 cut(s) 128, 701
FriOI GRGCYC 2 cut(s) 154, 373
Fsp4HI GCNGC 3 cut(s) 179, 199, 702
FspBI CTAG 1 cut(s) 845
GluI GCNGC 3 cut(s) 179, 199, 702
HaeIII GGCC 3 cut(s) 510, 549, 1381
HapII CCGG 4 cut(s) 147, 601, 1104, 1241
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 4 cut(s) 592, 751, 848, 1315
HpaII CCGG 4 cut(s) 147, 601, 1104, 1241
HphI GGTGA 3 cut(s) 929, 1007, 1376
Hpy166II GTNNAC 5 cut(s) 339, 350, 641, 1144, 1428
Hpy188I TCNGA 1 cut(s) 1397
Hpy8I GTNNAC 5 cut(s) 339, 350, 641, 1144, 1428
Hpy99I CGWCG 1 cut(s) 947
HpyAV CCTTC 4 cut(s) 902, 965, 995, 1022
HpyCH4III ACNGT 4 cut(s) 93, 336, 520, 645
HpyCH4IV ACGT 2 cut(s) 67, 442
HpyF10VI GCNNNNNNNGC 3 cut(s) 207, 222, 1180
HpyF3I CTNAG 3 cut(s) 231, 261, 1357
HpySE526I ACGT 2 cut(s) 67, 442
Ksp22I TGATCA 3 cut(s) 309, 979, 1018
LmnI GCTCC 2 cut(s) 157, 368
Lsp1109I GCAGC 3 cut(s) 165, 210, 713
MaeI CTAG 1 cut(s) 845
MaeII ACGT 2 cut(s) 67, 442
MaeIII GTNAC 4 cut(s) 4, 171, 800, 1120
MfeI CAATTG 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 154, 373
MlyI GAGTC 2 cut(s) 601, 1309
MseI TTAA 7 cut(s) 95, 251, 354, 498, 717, 795, 1110
MslI CAYNNNNRTG 2 cut(s) 774, 1014
MspI CCGG 4 cut(s) 147, 601, 1104, 1241
MspR9I CCNGG 4 cut(s) 147, 148, 602, 1104
MunI CAATTG 1 cut(s) 123
Mva1269I GAATGC 1 cut(s) 657
MwoI GCNNNNNNNGC 3 cut(s) 207, 222, 1180
NciI CCSGG 4 cut(s) 147, 148, 602, 1104
NcoI CCATGG 1 cut(s) 167
NlaIV GGNNCC 4 cut(s) 153, 370, 451, 1196
NmuCI GTSAC 2 cut(s) 800, 1120
NspI RCATGY 2 cut(s) 1102, 1121
PaeR7I CTCGAG 1 cut(s) 651
PagI TCATGA 4 cut(s) 490, 730, 1009, 1021
PciI ACATGT 1 cut(s) 1117
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PctI GAATGC 1 cut(s) 657
PfeI GAWTC 2 cut(s) 751, 848
PflMI CCANNNNNTGG 1 cut(s) 883
PkrI GCNGC 3 cut(s) 180, 200, 703
PleI GAGTC 2 cut(s) 600, 1309
PpsI GAGTC 2 cut(s) 600, 1309
PscI ACATGT 1 cut(s) 1117
Psp1406I AACGTT 1 cut(s) 442
PspN4I GGNNCC 4 cut(s) 153, 370, 451, 1196
PspPI GGNCC 1 cut(s) 547
RseI CAYNNNNRTG 2 cut(s) 774, 1014
SaqAI TTAA 7 cut(s) 95, 251, 354, 498, 717, 795, 1110
SatI GCNGC 3 cut(s) 179, 199, 702
Sau96I GGNCC 1 cut(s) 547
SchI GAGTC 2 cut(s) 601, 1309
ScrFI CCNGG 4 cut(s) 147, 148, 602, 1104
SduI GDGCHC 2 cut(s) 154, 373
SfcI CTRYAG 1 cut(s) 39
Sfr274I CTCGAG 1 cut(s) 651
SlaI CTCGAG 1 cut(s) 651
SmaI CCCGGG 1 cut(s) 148
SmiMI CAYNNNNRTG 2 cut(s) 774, 1014
SmlI CTYRAG 4 cut(s) 183, 211, 626, 651
SmoI CTYRAG 4 cut(s) 183, 211, 626, 651
SsiI CCGC 3 cut(s) 155, 386, 622
SspI AATATT 3 cut(s) 457, 840, 952
SspMI CTAG 1 cut(s) 845
StyD4I CCNGG 4 cut(s) 145, 146, 600, 1102
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 4 cut(s) 93, 336, 520, 645
TaiI ACGT 2 cut(s) 70, 445
TaqI TCGA 6 cut(s) 85, 271, 609, 652, 832, 1388
TfiI GAWTC 2 cut(s) 751, 848
Tru1I TTAA 7 cut(s) 95, 251, 354, 498, 717, 795, 1110
Tru9I TTAA 7 cut(s) 95, 251, 354, 498, 717, 795, 1110
TscAI CASTG 2 cut(s) 235, 1185
TseFI GTSAC 2 cut(s) 800, 1120
TseI GCWGC 3 cut(s) 178, 198, 701
Tsp45I GTSAC 2 cut(s) 800, 1120
TspDTI ATGAA 6 cut(s) 174, 747, 780, 876, 1014, 1107
TspMI CCCGGG 1 cut(s) 146
TspRI CASTG 2 cut(s) 235, 1185
Van91I CCANNNNNTGG 1 cut(s) 883
XceI RCATGY 2 cut(s) 1102, 1121
XhoI CTCGAG 1 cut(s) 651
XmaI CCCGGG 1 cut(s) 146
XmiI GTMKAC 4 cut(s) 338, 349, 640, 1143
XspI CTAG 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.