Rh4CG021500

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000144
Physical Location & Seq
Forward (+)
4258660 .. 4271397
12738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG021500.1

Sequence Viewer

Length: 606 bp
ATGGACATTGTGCCCTGGAAGAAACTACCTGACGATGTCATAGAGATGATTGTGCCACACCTGAGTGTACGTGATCGTATACGATTAAGCAGTGTTTGCAAGCCTTGGAGCTCAGCTCCTATGCGCACCGACATCCCTAGTGCTCAACATCAGCTTCCCTGGTTAATATTCCCTCAATCTTGCCATAGCTACTTGAGCTTTTTCGACCTCTGTGAAGGTAAAGTTGGCAAGTTGGACCTGTCTGAACCAGTTCAAGGAGGGTGGTTTCAAGGATCGTCAAAAGGTTGGATGATTATGATCACGGAAAAGGATCTGGATTCTACAATGTTTCTTGTGAATCCAATTTCAGGAGCCCAGCACCAACTTCCACATTTGAGCACGACTCCATCTTTCCAGAGCTTTGTAGACACAACCACATGGAAACGCTATGAACTTGATGCTTTGGCCAATAACCATGAAACTATTTCCAGGAAATATAATGTATTGCAATCATCTACTTGTTCATGTGCACCATTGAGTTCAGACTTCATAGACACCATGTGGAATGTAGTATATTGTGATAGAGATCATTCCCTAACTTATCGTTTTATTAGTCAAAAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

201

Amino Acids

23.02

Weight (kDa)

5.94

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 6 - 39 1.1e-06 F-box domain
F-box-like PF12937 6 - 38 4e-06 F-box-like
Beta-prop_KIB1-4 PF03478 67 - 179 9.8e-10 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 125
AccI GTMKAC 2 cut(s) 79, 405
AclWI GGATC 2 cut(s) 280, 318
AcoI YGGCCR 1 cut(s) 444
AdeI CACNNNGTG 1 cut(s) 540
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 2 cut(s) 254, 347
AgsI TTSAA 2 cut(s) 254, 269
AjnI CCWGG 3 cut(s) 14, 158, 467
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AleI CACNNNNGTG 1 cut(s) 63
AluBI AGCT 6 cut(s) 111, 116, 154, 189, 198, 399
AluI AGCT 6 cut(s) 111, 116, 154, 189, 198, 399
Alw21I GWGCWC 4 cut(s) 113, 145, 380, 511
Alw44I GTGCAC 1 cut(s) 507
AlwI GGATC 2 cut(s) 280, 318
AoxI GGCC 1 cut(s) 444
ApaLI GTGCAC 1 cut(s) 507
Asp700I GAANNNNTTC 1 cut(s) 249
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 235
AvaII GGWCC 1 cut(s) 235
BaeGI GKGCMC 2 cut(s) 15, 511
BalI TGGCCA 1 cut(s) 446
BanII GRGCYC 2 cut(s) 113, 355
Bbv12I GWGCWC 4 cut(s) 113, 145, 380, 511
BccI CCATC 1 cut(s) 394
BciT130I CCWGG 3 cut(s) 16, 160, 469
BclI TGATCA 1 cut(s) 297
BfaI CTAG 1 cut(s) 138
BlpI GCTNAGC 1 cut(s) 112
Bme1390I CCNGG 3 cut(s) 16, 160, 469
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 3 cut(s) 16, 160, 469
BmsI GCATC 1 cut(s) 427
BplI GAGNNNNNCTC 4 cut(s) 100, 132, 367, 399
Bpu1102I GCTNAGC 1 cut(s) 112
BpuEI CTTGAG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 71
BsaBI GATNNNNATC 2 cut(s) 296, 564
BsaJI CCNNGG 3 cut(s) 14, 104, 158
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 347
Bse1I ACTGG 1 cut(s) 248
Bse8I GATNNNNATC 2 cut(s) 296, 564
BseBI CCWGG 3 cut(s) 16, 160, 469
BseDI CCNNGG 3 cut(s) 14, 104, 158
BseGI GGATG 2 cut(s) 132, 294
BseJI GATNNNNATC 2 cut(s) 296, 564
BseLI CCNNNNNNNGG 2 cut(s) 254, 347
BseMII CTCAG 2 cut(s) 53, 126
BseNI ACTGG 1 cut(s) 248
BseSI GKGCMC 2 cut(s) 15, 511
BseYI CCCAGC 1 cut(s) 354
BshFI GGCC 1 cut(s) 446
BsiHKAI GWGCWC 4 cut(s) 113, 145, 380, 511
BslI CCNNNNNNNGG 2 cut(s) 254, 347
BsnI GGCC 1 cut(s) 446
Bsp1286I GDGCHC 6 cut(s) 15, 113, 145, 355, 380, 511
Bsp143I GATC 5 cut(s) 73, 272, 297, 310, 565
Bsp1720I GCTNAGC 1 cut(s) 112
BspANI GGCC 1 cut(s) 446
BspCNI CTCAG 2 cut(s) 54, 125
BspLI GGNNCC 1 cut(s) 352
BspPI GGATC 2 cut(s) 280, 318
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 14, 104, 158
BssMI GATC 5 cut(s) 73, 272, 297, 310, 565
BssNAI GTATAC 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 104
Bst1107I GTATAC 1 cut(s) 80
Bst2UI CCWGG 3 cut(s) 16, 160, 469
BstAPI GCANNNNNTGC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 2 cut(s) 62, 112
BstF5I GGATG 2 cut(s) 132, 294
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 5 cut(s) 76, 275, 300, 313, 568
BstMBI GATC 5 cut(s) 73, 272, 297, 310, 565
BstMWI GCNNNNNNNGC 2 cut(s) 96, 195
BstNI CCWGG 3 cut(s) 16, 160, 469
BstSCI CCNGG 3 cut(s) 14, 158, 467
BstSLI GKGCMC 2 cut(s) 15, 511
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BstZ17I GTATAC 1 cut(s) 80
BsuRI GGCC 1 cut(s) 446
BtsCI GGATG 2 cut(s) 132, 294
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 235
Csp6I GTAC 1 cut(s) 68
CspCI CAANNNNNGTGG 2 cut(s) 242, 277
CviAII CATG 4 cut(s) 417, 455, 504, 538
CviJI RGCY 9 cut(s) 103, 111, 116, 154, 189, 198, 353, 399, 446
CviKI_1 RGCY 9 cut(s) 103, 111, 116, 154, 189, 198, 353, 399, 446
CviQI GTAC 1 cut(s) 68
DdeI CTNAG 2 cut(s) 62, 112
DpnI GATC 5 cut(s) 75, 274, 299, 312, 567
DpnII GATC 5 cut(s) 73, 272, 297, 310, 565
DraIII CACNNNGTG 1 cut(s) 540
EaeI YGGCCR 1 cut(s) 444
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 104
Eco24I GRGCYC 2 cut(s) 113, 355
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 111
EcoRII CCWGG 3 cut(s) 14, 158, 467
EcoT14I CCWWGG 1 cut(s) 104
EcoT38I GRGCYC 2 cut(s) 113, 355
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 4 cut(s) 420, 458, 507, 541
FatI CATG 4 cut(s) 416, 454, 503, 537
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 2 cut(s) 79, 405
FokI GGATG 2 cut(s) 119, 301
FriOI GRGCYC 2 cut(s) 113, 355
FspAI RTGCGCAY 1 cut(s) 125
FspBI CTAG 1 cut(s) 138
FspI TGCGCA 1 cut(s) 125
GlaI GCGC 1 cut(s) 125
GsaI CCCAGC 1 cut(s) 358
HaeIII GGCC 1 cut(s) 446
HhaI GCGC 1 cut(s) 126
Hin1II CATG 4 cut(s) 420, 458, 507, 541
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HinfI GANTC 3 cut(s) 317, 337, 382
Hpy166II GTNNAC 4 cut(s) 68, 80, 406, 509
Hpy188I TCNGA 2 cut(s) 244, 523
Hpy188III TCNNGA 3 cut(s) 314, 348, 394
Hpy8I GTNNAC 4 cut(s) 68, 80, 406, 509
HpyAV CCTTC 1 cut(s) 209
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 3 cut(s) 99, 487, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 195
HpyF3I CTNAG 2 cut(s) 62, 112
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 4 cut(s) 420, 458, 507, 541
HspAI GCGC 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 5 cut(s) 73, 272, 297, 310, 565
LmnI GCTCC 3 cut(s) 108, 121, 350
LweI GCATC 1 cut(s) 427
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 70
MalI GATC 5 cut(s) 75, 274, 299, 312, 567
MboI GATC 5 cut(s) 73, 272, 297, 310, 565
MboII GAAGA 1 cut(s) 31
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 6 cut(s) 15, 113, 145, 355, 380, 511
MlsI TGGCCA 1 cut(s) 446
MluCI AATT 1 cut(s) 342
MluNI TGGCCA 1 cut(s) 446
MlyI GAGTC 1 cut(s) 376
MmeI TCCRAC 2 cut(s) 213, 266
MnlI CCTC 3 cut(s) 183, 218, 251
Mox20I TGGCCA 1 cut(s) 446
MroXI GAANNNNTTC 1 cut(s) 249
MscI TGGCCA 1 cut(s) 446
MseI TTAA 3 cut(s) 86, 164, 604
MslI CAYNNNNRTG 2 cut(s) 44, 63
Msp20I TGGCCA 1 cut(s) 446
MspR9I CCNGG 3 cut(s) 16, 160, 469
MvaI CCWGG 3 cut(s) 16, 160, 469
MwoI GCNNNNNNNGC 2 cut(s) 96, 195
NdeII GATC 5 cut(s) 73, 272, 297, 310, 565
NlaIII CATG 4 cut(s) 420, 458, 507, 541
NlaIV GGNNCC 1 cut(s) 352
NsbI TGCGCA 1 cut(s) 125
OliI CACNNNNGTG 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 2 cut(s) 317, 337
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 467
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Ppu21I YACGTR 1 cut(s) 71
Psp124BI GAGCTC 1 cut(s) 113
Psp6I CCWGG 3 cut(s) 14, 158, 467
PspFI CCCAGC 1 cut(s) 354
PspGI CCWGG 3 cut(s) 14, 158, 467
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 1 cut(s) 235
PsuI RGATCY 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 35
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
RseI CAYNNNNRTG 2 cut(s) 44, 63
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 3 cut(s) 86, 164, 604
Sau3AI GATC 5 cut(s) 73, 272, 297, 310, 565
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 1 cut(s) 376
ScrFI CCNGG 3 cut(s) 16, 160, 469
SduI GDGCHC 6 cut(s) 15, 113, 145, 355, 380, 511
SfaNI GCATC 1 cut(s) 427
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 44, 63
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 1 cut(s) 342
SspI AATATT 1 cut(s) 168
SspMI CTAG 1 cut(s) 138
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 3 cut(s) 14, 158, 467
StyI CCWWGG 1 cut(s) 104
TaiI ACGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 204
TasI AATT 1 cut(s) 342
TfiI GAWTC 2 cut(s) 317, 337
Tru1I TTAA 3 cut(s) 86, 164, 604
Tru9I TTAA 3 cut(s) 86, 164, 604
TscAI CASTG 1 cut(s) 97
TspDTI ATGAA 4 cut(s) 444, 471, 492, 517
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 97
Tth111I GACNNNGTC 1 cut(s) 35
VneI GTGCAC 1 cut(s) 507
VpaK11BI GGWCC 1 cut(s) 235
XmiI GTMKAC 2 cut(s) 79, 405
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.