Rorug05G0553900

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000144
Physical Location & Seq
Forward (+)
74154180 .. 74155091
912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0553900.1

Sequence Viewer

Length: 912 bp
ATGGGGTTCAGCTTATCTGTCCTGGGTAAATTCTTCAAATTGGGTCTTCAACCAAATGTTATTACCTTCTCCACTCTAATCAACGGCTTTGTTCTCCACAATCAAGTGCCTGAGGCTGCACGAATTTTCACCAAAATGCTGGAGGCAGGTCATTCTAAGCCCGATGTGGTTACTTTCAACACTCTAATAAAGGGCTTTTGCGTGACGGGGAACAGCCGTGCAGCGATTCAGTTACTTAGGAACATGGAACAAAGAGGATGCGAGCCTGACATAGTTTCCTATAGCATCATCATTGACTGTCTTTGTAAGGATACACTTGTTGTTGATGCAATGAACCTCTTCTCAGAAATGATAAGTAAAGGTATTGCTCCAAACGTTGTTACTTACACCTCTTTAATTCATGGAGTTTGCAATATAGGACAGTGGAAACAAGCTACGAGGTTACTTCATGAAATGTTGAATCAAGGTATCTTTCCAGATGTCGTCACATTCAATGTCTTGGTTGATACATTCTGTAAGGAAGGGATGGTTGTGGAAGCCAAAACTGTGGTTGACATGATGATTCAAAGACATATTGAGCCTGATACGATTACATACAGCTCGCTTATGGATGGTTACCGTTTGCGAGGAGATATGGACAAGGCGAAACAAGTCTTTGATCTGATGATTAGCAAGGGATCCATGGTCAATGTTTGGAGTTGTAACATATTGATAAACGGATACTGTAAGCAGAAAAAGATCGATGAGGCCAATAAGGTTTTTCAGGAAATGACTTGTTGGGAGCTTGTTCCAGACACCATTACTTATAACACTCTTATTGATGGTTTTTACAAAGCAGGGAGAATACAAGATGCAGAAAAGCTGTTCTCTGAGATGCAAGGTTGTGGCCAACGCAATGTTTTAAAACGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

303

Amino Acids

34.09

Weight (kDa)

6.93

Isoelectric Point (pI)

25.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 15 - 46 3.3e-07 PPR repeat
PPR_1 PF12854 53 - 83 9.4e-10 PPR repeat
PPR_3 PF13812 53 - 100 1.5e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 54 - 103 3.4e-19 PPR repeat family
PPR PF01535 57 - 87 8.9e-07 PPR repeat
PPR_1 PF12854 85 - 117 1.1e-11 PPR repeat
PPR_2 PF13041 90 - 138 1.4e-16 PPR repeat family
PPR PF01535 92 - 122 2.9e-06 PPR repeat
PPR_long PF17177 108 - 205 2.7e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 113 - 172 1.9e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 120 - 152 4.8e-11 PPR repeat
PPR_2 PF13041 124 - 173 1.4e-17 PPR repeat family
MRP-S27 PF10037 128 - 191 2.6e-08 Mitochondrial 28S ribosomal protein S27
PPR_1 PF12854 156 - 187 7.8e-11 PPR repeat
PPR_2 PF13041 159 - 206 1.9e-13 PPR repeat family
TPR_24 PF23276 163 - 257 6.6e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 191 - 222 1.4e-07 PPR repeat
PPR PF01535 197 - 226 8.5e-07 PPR repeat
PPR_3 PF13812 225 - 274 1.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 226 - 258 2.9e-08 PPR repeat
PPR_2 PF13041 230 - 278 1.3e-17 PPR repeat family
PPR PF01535 233 - 258 2.5e-07 PPR repeat
PPR_1 PF12854 261 - 293 1.6e-12 PPR repeat
PPR_2 PF13041 264 - 294 1.4e-09 PPR repeat family
PPR PF01535 267 - 296 5.4e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
Acc36I ACCTGC 1 cut(s) 137
AclI AACGTT 1 cut(s) 375
AclWI GGATC 2 cut(s) 672, 685
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 2 cut(s) 29, 123
AgsI TTSAA 6 cut(s) 37, 50, 178, 460, 493, 566
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 5 cut(s) 12, 434, 600, 784, 862
AluI AGCT 5 cut(s) 12, 434, 600, 784, 862
AlwI GGATC 2 cut(s) 672, 685
AoxI GGCC 2 cut(s) 747, 886
ApeKI GCWGC 2 cut(s) 116, 221
ApoI RAATTY 2 cut(s) 29, 123
Asp700I GAANNNNTTC 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 121
AxyI CCTNAGG 1 cut(s) 111
BaeI ACNNNNGTAYC 2 cut(s) 303, 336
BalI TGGCCA 1 cut(s) 888
BamHI GGATCC 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 103, 233
BccI CCATC 3 cut(s) 520, 605, 815
BceAI ACGGC 2 cut(s) 100, 201
BciT130I CCWGG 1 cut(s) 23
BciVI GTATCC 2 cut(s) 304, 713
BfmI CTRYAG 1 cut(s) 280
BfuAI ACCTGC 1 cut(s) 137
BfuI GTATCC 2 cut(s) 304, 713
BisI GCNGC 2 cut(s) 117, 222
BlsI GCNGC 2 cut(s) 118, 223
Bme1390I CCNGG 1 cut(s) 23
BmiI GGNNCC 1 cut(s) 679
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 5 cut(s) 248, 294, 316, 841, 864
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 161
Bsa29I ATCGAT 1 cut(s) 741
BsaJI CCNNGG 2 cut(s) 22, 681
Bse21I CCTNAGG 1 cut(s) 111
Bse3DI GCAATG 2 cut(s) 336, 901
BseBI CCWGG 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 22, 681
BseGI GGATG 3 cut(s) 263, 531, 616
BseMI GCAATG 2 cut(s) 336, 901
BseMII CTCAG 3 cut(s) 102, 357, 861
BseRI GAGGAG 1 cut(s) 642
BseXI GCAGC 2 cut(s) 103, 233
BsgI GTGCAG 2 cut(s) 102, 240
BshFI GGCC 2 cut(s) 749, 888
BshVI ATCGAT 1 cut(s) 741
BsnI GGCC 2 cut(s) 749, 888
Bsp143I GATC 3 cut(s) 658, 677, 738
Bsp19I CCATGG 1 cut(s) 681
BspANI GGCC 2 cut(s) 749, 888
BspCNI CTCAG 3 cut(s) 103, 356, 862
BspDI ATCGAT 1 cut(s) 741
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 679
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 2 cut(s) 672, 685
BsrDI GCAATG 2 cut(s) 336, 901
BssECI CCNNGG 2 cut(s) 22, 681
BssMI GATC 3 cut(s) 658, 677, 738
BssT1I CCWWGG 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 5 cut(s) 299, 423, 547, 620, 725
Bst6I CTCTTC 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 263, 602
BstDEI CTNAG 5 cut(s) 111, 156, 236, 343, 870
BstDSI CCRYGG 1 cut(s) 681
BstEII GGTNACC 1 cut(s) 614
BstF5I GGATG 3 cut(s) 263, 531, 616
BstKTI GATC 3 cut(s) 661, 680, 741
BstMBI GATC 3 cut(s) 658, 677, 738
BstNI CCWGG 1 cut(s) 23
BstPI GGTNACC 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 280
BstV1I GCAGC 2 cut(s) 103, 233
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 1 cut(s) 677
BstXI CCANNNNNNTGG 2 cut(s) 139, 547
BstYI RGATCY 1 cut(s) 677
Bsu15I ATCGAT 1 cut(s) 741
Bsu36I CCTNAGG 1 cut(s) 111
BsuI GTATCC 2 cut(s) 304, 713
BsuRI GGCC 2 cut(s) 749, 888
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 1 cut(s) 681
BtsCI GGATG 3 cut(s) 263, 531, 616
BtsIMutI CAGTG 1 cut(s) 428
BveI ACCTGC 1 cut(s) 137
Cac8I GCNNGC 2 cut(s) 263, 602
CciI TCATGA 1 cut(s) 448
ClaI ATCGAT 1 cut(s) 741
CviAII CATG 5 cut(s) 244, 401, 449, 556, 682
DdeI CTNAG 5 cut(s) 111, 156, 236, 343, 870
DpnI GATC 3 cut(s) 660, 679, 740
DpnII GATC 3 cut(s) 658, 677, 738
DraI TTTAAA 1 cut(s) 903
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 681
Eco81I CCTNAGG 1 cut(s) 111
Eco91I GGTNACC 1 cut(s) 614
EcoO65I GGTNACC 1 cut(s) 614
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 5 cut(s) 247, 404, 452, 559, 685
FatI CATG 5 cut(s) 243, 400, 448, 555, 681
Fnu4HI GCNGC 2 cut(s) 117, 222
FokI GGATG 3 cut(s) 270, 538, 623
Fsp4HI GCNGC 2 cut(s) 117, 222
GluI GCNGC 2 cut(s) 117, 222
GsuI CTGGAG 1 cut(s) 161
HaeIII GGCC 2 cut(s) 749, 888
Hin1II CATG 5 cut(s) 247, 404, 452, 559, 685
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 3 cut(s) 226, 460, 562
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 3 cut(s) 346, 663, 871
Hpy188III TCNNGA 4 cut(s) 449, 476, 764, 791
Hpy8I GTNNAC 1 cut(s) 553
HpyAV CCTTC 2 cut(s) 76, 515
HpyCH4III ACNGT 5 cut(s) 299, 423, 547, 620, 725
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 6 cut(s) 119, 221, 329, 411, 854, 877
HpyF3I CTNAG 5 cut(s) 111, 156, 236, 343, 870
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 5 cut(s) 247, 404, 452, 559, 685
Kzo9I GATC 3 cut(s) 658, 677, 738
LmnI GCTCC 2 cut(s) 373, 781
Lsp1109I GCAGC 2 cut(s) 103, 233
LweI GCATC 5 cut(s) 248, 294, 316, 841, 864
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 8 cut(s) 169, 202, 231, 379, 441, 484, 614, 701
MalI GATC 3 cut(s) 660, 679, 740
MboI GATC 3 cut(s) 658, 677, 738
MboII GAAGA 3 cut(s) 25, 38, 331
MflI RGATCY 1 cut(s) 677
MlsI TGGCCA 1 cut(s) 888
MluCI AATT 4 cut(s) 29, 38, 123, 396
MluNI TGGCCA 1 cut(s) 888
MnlI CCTC 8 cut(s) 106, 136, 248, 347, 400, 432, 620, 739
Mox20I TGGCCA 1 cut(s) 888
MroXI GAANNNNTTC 1 cut(s) 338
MscI TGGCCA 1 cut(s) 888
MseI TTAA 2 cut(s) 395, 902
MslI CAYNNNNRTG 1 cut(s) 134
Msp20I TGGCCA 1 cut(s) 888
MspR9I CCNGG 1 cut(s) 23
MvaI CCWGG 1 cut(s) 23
NcoI CCATGG 1 cut(s) 681
NdeII GATC 3 cut(s) 658, 677, 738
NlaIII CATG 5 cut(s) 247, 404, 452, 559, 685
NlaIV GGNNCC 1 cut(s) 679
NmuCI GTSAC 2 cut(s) 202, 484
PagI TCATGA 1 cut(s) 448
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 3 cut(s) 226, 460, 562
PkrI GCNGC 2 cut(s) 118, 223
PsiI TTATAA 1 cut(s) 807
Psp1406I AACGTT 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 21
PspEI GGTNACC 1 cut(s) 614
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 679
PsuI RGATCY 1 cut(s) 677
RseI CAYNNNNRTG 1 cut(s) 134
SaqAI TTAA 2 cut(s) 395, 902
SatI GCNGC 2 cut(s) 117, 222
Sau3AI GATC 3 cut(s) 658, 677, 738
ScrFI CCNGG 1 cut(s) 23
SfaNI GCATC 5 cut(s) 248, 294, 316, 841, 864
SfcI CTRYAG 1 cut(s) 280
SmiMI CAYNNNNRTG 1 cut(s) 134
Sse9I AATT 4 cut(s) 29, 38, 123, 396
StyD4I CCNGG 1 cut(s) 21
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 5 cut(s) 299, 423, 547, 620, 725
TaiI ACGT 1 cut(s) 378
TaqI TCGA 1 cut(s) 741
TasI AATT 4 cut(s) 29, 38, 123, 396
TfiI GAWTC 3 cut(s) 226, 460, 562
Tru1I TTAA 2 cut(s) 395, 902
Tru9I TTAA 2 cut(s) 395, 902
TscAI CASTG 1 cut(s) 428
TseFI GTSAC 2 cut(s) 202, 484
TseI GCWGC 2 cut(s) 116, 221
Tsp45I GTSAC 2 cut(s) 202, 484
TspDTI ATGAA 4 cut(s) 347, 389, 437, 465
TspGWI ACGGA 1 cut(s) 732
TspRI CASTG 1 cut(s) 428
XapI RAATTY 2 cut(s) 29, 123
XmnI GAANNNNTTC 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.