Rh6BG063200

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000144
Physical Location & Seq
Forward (+)
9867298 .. 9868467
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG063200.1

Sequence Viewer

Length: 1170 bp
ATGATCGAGTCTCTATTCGCTTGCTTCAATCCTCGATGCAATACGAGCAAGCTTAGGCTGCTAAAGAGAAAAAAACTCCCAAAGAAATTAGAGAGAGAGCAGAAGGAGAATGAAGATGATAAGTACAGAAACCAAACCAGTTCTAATGAATGTACCTCTTGGCAGCCACCACCTCCTGATATCATGGAGCAAGTTCTGCCGCGTGTCAGAGTAAGTGATCAAATAAGGCTAAGTATTGCCTGCAAGTCTTGGATGTCAATCGTTATGCGTAGAGACATCCGCAGTGCTTCCCATGAATTCCCATGGTTACTACTCCCTCAAACCCCACATTGCAGCAACAAGTACGTAAGCTTTGCCAGCGTGTCAGAGGGGAGAGTTGTCAAGTTGAAGCTACCTAAGCAAGTTCGTGGATGGTGGATTTATGGGTCTTCTAAAGGTTGGTTGATCATGATCAAGGAAAGAGGTCTAAATTCCAAGATGTGTCTACTTAACCCAATTTCAGGAGCCCTACTCCAACTTCCACCCTTGAGAACACTTCCTTTTCTGAAAGATTTTGTGAAAACCGAGGCCTGGAAACTTTTTGGTGCCAATGCATTCGACTTAAGCATTGCACTATCAACCTCTGATGGAATTGCTTTAGATTCAAAGCGTTGTACGGTAGCAGCAGTTTTTAATGATAAGTCGAAATTGAGTTTGTGCAGACCTGGAGACAGAACATGGAGTGACTTTCAAGTATTAGATACTAATCAAAACGACTGGATTGCTGATTTATTGTTTTCTTCCGGCAGTCTATATGTTTTGGTTCGTAGTAGTCAAAAGGAAAGCTTTGTAGACTCAGTTACTCAAACCTTGAACTTTGGCTTTGGAGATGCAGAAAATTTGAAAATGAAGTTGGTCTACGACAAGCATGAAAACAGGAACGTGAACGTTAATGAATGTCATAGTGACTATAAGATCGTTTACAATGCATTATACTTCTCAAGATTGTTAGAATCAACCAGCAATGAAGTCTTATTGATCCATCAAATGGTAGATTATGTTGTGGGAAGAAGAGAGGATATTATTGGTGACAATAATGAGAATAATGGAGGCGGCAATATGGAGAATGATGATGGTGAGCAACACATTCATCATGATATGACGACGGAAATCTTGAGGTTGATGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

389

Amino Acids

44.58

Weight (kDa)

8.47

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 118 - 342 1.4e-20 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 584
AccB7I CCANNNNNTGG 1 cut(s) 1027
AccI GTMKAC 3 cut(s) 484, 831, 897
AccII CGCG 1 cut(s) 202
AciI CCGC 3 cut(s) 200, 280, 1092
AclI AACGTT 1 cut(s) 927
AclWI GGATC 1 cut(s) 1012
AcsI RAATTY 3 cut(s) 296, 469, 877
AfaI GTAC 4 cut(s) 125, 154, 344, 655
AfiI CCNNNNNNNGG 3 cut(s) 500, 570, 1027
AflII CTTAAG 1 cut(s) 601
AgsI TTSAA 6 cut(s) 28, 388, 645, 731, 853, 883
AjnI CCWGG 2 cut(s) 569, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 875, 907
AluBI AGCT 4 cut(s) 52, 351, 391, 825
AluI AGCT 4 cut(s) 52, 351, 391, 825
Alw26I GTCTC 3 cut(s) 15, 267, 702
AlwI GGATC 1 cut(s) 1012
AoxI GGCC 1 cut(s) 567
ApeKI GCWGC 4 cut(s) 58, 163, 333, 662
ApoI RAATTY 3 cut(s) 296, 469, 877
AsuHPI GGTGA 2 cut(s) 1079, 1127
BanI GGYRCC 1 cut(s) 584
BanII GRGCYC 1 cut(s) 508
BarI GAAGNNNNNNTAC 2 cut(s) 881, 913
BbsI GAAGAC 1 cut(s) 420
BbvI GCAGC 4 cut(s) 45, 175, 345, 674
BccI CCATC 5 cut(s) 405, 620, 1029, 1106, 1156
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 2 cut(s) 571, 705
BclI TGATCA 3 cut(s) 217, 444, 450
BcoDI GTCTC 3 cut(s) 15, 267, 702
BfrI CTTAAG 1 cut(s) 601
BisI GCNGC 6 cut(s) 59, 164, 200, 334, 663, 1093
BlsI GCNGC 6 cut(s) 60, 165, 201, 335, 664, 1094
Bme1390I CCNGG 2 cut(s) 571, 705
BmiI GGNNCC 2 cut(s) 505, 586
BmrFI CCNGG 2 cut(s) 571, 705
BmsI GCATC 2 cut(s) 26, 859
BpiI GAAGAC 1 cut(s) 420
BplI GAGNNNNNCTC 2 cut(s) 495, 527
BpmI CTGGAG 1 cut(s) 726
Bpu10I CCTNAGC 2 cut(s) 53, 396
BpuEI CTTGAG 2 cut(s) 547, 964
BsaAI YACGTR 1 cut(s) 346
BsaBI GATNNNNATC 3 cut(s) 257, 449, 744
BsaJI CCNNGG 2 cut(s) 302, 564
Bsc4I CCNNNNNNNGG 3 cut(s) 500, 570, 1027
Bse1I ACTGG 2 cut(s) 138, 761
Bse3DI GCAATG 3 cut(s) 328, 606, 1009
Bse8I GATNNNNATC 3 cut(s) 257, 449, 744
BseBI CCWGG 2 cut(s) 571, 705
BseDI CCNNGG 2 cut(s) 302, 564
BseGI GGATG 3 cut(s) 258, 276, 416
BseJI GATNNNNATC 3 cut(s) 257, 449, 744
BseLI CCNNNNNNNGG 3 cut(s) 500, 570, 1027
BseMI GCAATG 3 cut(s) 328, 606, 1009
BseMII CTCAG 1 cut(s) 849
BseNI ACTGG 2 cut(s) 138, 761
BseXI GCAGC 4 cut(s) 45, 175, 345, 674
BsgI GTGCAG 1 cut(s) 718
Bsh1236I CGCG 1 cut(s) 202
BshFI GGCC 1 cut(s) 569
BshNI GGYRCC 1 cut(s) 584
BsiSI CCGG 1 cut(s) 783
BslI CCNNNNNNNGG 3 cut(s) 500, 570, 1027
BsmAI GTCTC 3 cut(s) 15, 267, 702
BsmI GAATGC 1 cut(s) 593
BsnI GGCC 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 6 cut(s) 3, 217, 444, 450, 954, 1017
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 3 cut(s) 200, 280, 1092
BspANI GGCC 1 cut(s) 569
BspCNI CTCAG 1 cut(s) 848
BspFNI CGCG 1 cut(s) 202
BspHI TCATGA 2 cut(s) 447, 1132
BspLI GGNNCC 2 cut(s) 505, 586
BspPI GGATC 1 cut(s) 1012
BspT107I GGYRCC 1 cut(s) 584
BspTI CTTAAG 1 cut(s) 601
BsrDI GCAATG 3 cut(s) 328, 606, 1009
BsrI ACTGG 2 cut(s) 138, 761
BssECI CCNNGG 2 cut(s) 302, 564
BssMI GATC 6 cut(s) 3, 217, 444, 450, 954, 1017
BssT1I CCWWGG 1 cut(s) 302
Bst2UI CCWGG 2 cut(s) 571, 705
Bst4CI ACNGT 1 cut(s) 658
Bst6I CTCTTC 1 cut(s) 1045
BstAFI CTTAAG 1 cut(s) 601
BstAPI GCANNNNNTGC 1 cut(s) 196
BstBAI YACGTR 1 cut(s) 346
BstC8I GCNNGC 4 cut(s) 22, 50, 241, 358
BstDEI CTNAG 4 cut(s) 53, 230, 396, 835
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 3 cut(s) 258, 276, 416
BstFNI CGCG 1 cut(s) 202
BstKTI GATC 6 cut(s) 6, 220, 447, 453, 957, 1020
BstMAI GTCTC 3 cut(s) 15, 267, 702
BstMBI GATC 6 cut(s) 3, 217, 444, 450, 954, 1017
BstMWI GCNNNNNNNGC 5 cut(s) 45, 58, 196, 357, 397
BstNI CCWGG 2 cut(s) 571, 705
BstSCI CCNGG 2 cut(s) 569, 703
BstSNI TACGTA 1 cut(s) 346
BstUI CGCG 1 cut(s) 202
BstV1I GCAGC 4 cut(s) 45, 175, 345, 674
BstV2I GAAGAC 1 cut(s) 420
BsuRI GGCC 1 cut(s) 569
BtgI CCRYGG 1 cut(s) 302
BtsCI GGATG 3 cut(s) 258, 276, 416
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 289
Cac8I GCNNGC 4 cut(s) 22, 50, 241, 358
CciI TCATGA 2 cut(s) 447, 1132
Csp6I GTAC 4 cut(s) 124, 153, 343, 654
CviAII CATG 7 cut(s) 184, 293, 303, 448, 717, 908, 1133
CviQI GTAC 4 cut(s) 124, 153, 343, 654
DdeI CTNAG 4 cut(s) 53, 230, 396, 835
DpnI GATC 6 cut(s) 5, 219, 446, 452, 956, 1019
DpnII GATC 6 cut(s) 3, 217, 444, 450, 954, 1017
Eam1104I CTCTTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 1045
Eco105I TACGTA 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 302
Eco147I AGGCCT 1 cut(s) 569
Eco24I GRGCYC 1 cut(s) 508
Eco32I GATATC 1 cut(s) 181
EcoRI GAATTC 1 cut(s) 296
EcoRII CCWGG 2 cut(s) 569, 703
EcoRV GATATC 1 cut(s) 181
EcoT14I CCWWGG 1 cut(s) 302
EcoT22I ATGCAT 2 cut(s) 595, 970
EcoT38I GRGCYC 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 302
FaeI CATG 7 cut(s) 187, 296, 306, 451, 720, 911, 1136
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FatI CATG 7 cut(s) 183, 292, 302, 447, 716, 907, 1132
FbaI TGATCA 3 cut(s) 217, 444, 450
FblI GTMKAC 3 cut(s) 484, 831, 897
Fnu4HI GCNGC 6 cut(s) 59, 164, 200, 334, 663, 1093
FokI GGATG 3 cut(s) 263, 265, 423
FriOI GRGCYC 1 cut(s) 508
Fsp4HI GCNGC 6 cut(s) 59, 164, 200, 334, 663, 1093
GluI GCNGC 6 cut(s) 59, 164, 200, 334, 663, 1093
GsuI CTGGAG 1 cut(s) 726
HaeIII GGCC 1 cut(s) 569
HapII CCGG 1 cut(s) 783
Hin1II CATG 7 cut(s) 187, 296, 306, 451, 720, 911, 1136
HindIII AAGCTT 3 cut(s) 50, 349, 823
HinfI GANTC 4 cut(s) 8, 641, 833, 992
HpaII CCGG 1 cut(s) 783
HphI GGTGA 2 cut(s) 1079, 1127
Hpy166II GTNNAC 5 cut(s) 485, 832, 898, 925, 961
Hpy188I TCNGA 4 cut(s) 209, 367, 546, 625
Hpy188III TCNNGA 6 cut(s) 176, 448, 501, 981, 1133, 1153
Hpy8I GTNNAC 5 cut(s) 485, 832, 898, 925, 961
Hpy99I CGWCG 1 cut(s) 1147
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 1 cut(s) 658
HpyCH4IV ACGT 3 cut(s) 345, 921, 927
HpyCH4V TGCA 8 cut(s) 39, 243, 333, 593, 611, 699, 872, 968
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 58, 196, 357, 397
HpyF3I CTNAG 4 cut(s) 53, 230, 396, 835
HpySE526I ACGT 3 cut(s) 345, 921, 927
Hsp92II CATG 7 cut(s) 187, 296, 306, 451, 720, 911, 1136
Ksp22I TGATCA 3 cut(s) 217, 444, 450
Kzo9I GATC 6 cut(s) 3, 217, 444, 450, 954, 1017
LmnI GCTCC 2 cut(s) 187, 503
Lsp1109I GCAGC 4 cut(s) 45, 175, 345, 674
LweI GCATC 2 cut(s) 26, 859
MaeII ACGT 3 cut(s) 345, 921, 927
MaeIII GTNAC 5 cut(s) 306, 722, 838, 944, 1067
MalI GATC 6 cut(s) 5, 219, 446, 452, 956, 1019
MboI GATC 6 cut(s) 3, 217, 444, 450, 954, 1017
MboII GAAGA 5 cut(s) 125, 420, 771, 1059, 1062
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 7 cut(s) 86, 296, 469, 495, 630, 686, 877
MlyI GAGTC 2 cut(s) 17, 827
MmeI TCCRAC 1 cut(s) 538
Mph1103I ATGCAT 2 cut(s) 595, 970
MseI TTAA 4 cut(s) 489, 602, 672, 930
MspCI CTTAAG 1 cut(s) 601
MspI CCGG 1 cut(s) 783
MspR9I CCNGG 2 cut(s) 571, 705
Mva1269I GAATGC 1 cut(s) 593
MvaI CCWGG 2 cut(s) 571, 705
MvnI CGCG 1 cut(s) 202
MwoI GCNNNNNNNGC 5 cut(s) 45, 58, 196, 357, 397
NcoI CCATGG 1 cut(s) 302
NdeII GATC 6 cut(s) 3, 217, 444, 450, 954, 1017
NlaIII CATG 7 cut(s) 187, 296, 306, 451, 720, 911, 1136
NlaIV GGNNCC 2 cut(s) 505, 586
NmuCI GTSAC 3 cut(s) 722, 944, 1067
NsiI ATGCAT 2 cut(s) 595, 970
PagI TCATGA 2 cut(s) 447, 1132
PceI AGGCCT 1 cut(s) 569
PctI GAATGC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 641, 992
PflMI CCANNNNNTGG 1 cut(s) 1027
PkrI GCNGC 6 cut(s) 60, 165, 201, 335, 664, 1094
PleI GAGTC 2 cut(s) 16, 827
PpsI GAGTC 2 cut(s) 16, 827
Ppu21I YACGTR 1 cut(s) 346
Psp1406I AACGTT 1 cut(s) 927
Psp6I CCWGG 2 cut(s) 569, 703
PspGI CCWGG 2 cut(s) 569, 703
PspN4I GGNNCC 2 cut(s) 505, 586
RsaI GTAC 4 cut(s) 125, 154, 344, 655
RsaNI GTAC 4 cut(s) 124, 153, 343, 654
SaqAI TTAA 4 cut(s) 489, 602, 672, 930
SatI GCNGC 6 cut(s) 59, 164, 200, 334, 663, 1093
Sau3AI GATC 6 cut(s) 3, 217, 444, 450, 954, 1017
SchI GAGTC 2 cut(s) 17, 827
ScrFI CCNGG 2 cut(s) 571, 705
SduI GDGCHC 1 cut(s) 508
SfaNI GCATC 2 cut(s) 26, 859
SmlI CTYRAG 4 cut(s) 526, 601, 979, 1153
SmoI CTYRAG 4 cut(s) 526, 601, 979, 1153
SnaBI TACGTA 1 cut(s) 346
Sse9I AATT 7 cut(s) 86, 296, 469, 495, 630, 686, 877
SseBI AGGCCT 1 cut(s) 569
SsiI CCGC 3 cut(s) 200, 280, 1092
StuI AGGCCT 1 cut(s) 569
StyD4I CCNGG 2 cut(s) 569, 703
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 1 cut(s) 658
TaiI ACGT 3 cut(s) 348, 924, 930
TaqI TCGA 4 cut(s) 6, 34, 597, 683
TasI AATT 7 cut(s) 86, 296, 469, 495, 630, 686, 877
TatI WGTACW 1 cut(s) 123
TauI GCSGC 2 cut(s) 202, 1095
TfiI GAWTC 2 cut(s) 641, 992
Tru1I TTAA 4 cut(s) 489, 602, 672, 930
Tru9I TTAA 4 cut(s) 489, 602, 672, 930
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 3 cut(s) 722, 944, 1067
TseI GCWGC 4 cut(s) 58, 163, 333, 662
Tsp45I GTSAC 3 cut(s) 722, 944, 1067
TspDTI ATGAA 8 cut(s) 126, 162, 309, 902, 924, 948, 1020, 1118
TspGWI ACGGA 1 cut(s) 1160
TspRI CASTG 1 cut(s) 289
Van91I CCANNNNNTGG 1 cut(s) 1027
Vha464I CTTAAG 1 cut(s) 601
XapI RAATTY 3 cut(s) 296, 469, 877
XmiI GTMKAC 3 cut(s) 484, 831, 897
Zsp2I ATGCAT 2 cut(s) 595, 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.